BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Paeonia23_contig00040366
         (810 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AHL44980.1| 4-coumarate:coenzyme A ligase 4 [Fraxinus mandshu...   314   3e-83
ref|XP_007029575.1| 4-coumarate:CoA ligase 3 [Theobroma cacao] g...   310   5e-82
gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]        303   4e-80
gb|AGO89321.1| Ca4CL4 [Salix arbutifolia]                             303   5e-80
gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]                  297   3e-78
gb|AFC89538.1| 4-coumarate: coenzyme A ligase 2 [Populus tomentosa]   295   1e-77
dbj|BAG82851.1| 4-coumarate:CoA ligase [Ipomoea batatas]              295   1e-77
gb|AAK58909.1| 4-coumarate:CoA ligase 4 [Populus trichocarpa x P...   293   4e-77
gb|ABA40922.1| 4-coumaroyl CoA ligase [Camellia sinensis]             292   9e-77
ref|XP_002325815.1| 4-coumarate-CoA ligase family protein [Popul...   291   1e-76
gb|AAC24504.1| 4-coumarate:CoA ligase [Populus tremuloides]           291   2e-76
gb|AEX32786.1| coumaroyl-CoA ligase [Vitis vinifera]                  290   3e-76
ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis ...   290   3e-76
gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum]          289   7e-76
gb|AFG30056.1| 4-coumarate: CoA ligase [Malus hybrid cultivar]        289   1e-75
gb|EYU45376.1| hypothetical protein MIMGU_mgv1a003734mg [Mimulus...   287   3e-75
gb|ACZ06243.1| 4-coumarate:CoA ligase 2 [Gossypium hirsutum]          287   3e-75
ref|XP_004307864.1| PREDICTED: LOW QUALITY PROTEIN: 4-coumarate-...   287   4e-75
gb|ADE96997.1| p-coumarate:CoA-ligase 3 [Sorbus aucuparia]            285   2e-74
gb|ADU15550.1| 4CL [Gossypium hirsutum]                               283   4e-74

>gb|AHL44980.1| 4-coumarate:coenzyme A ligase 4 [Fraxinus mandshurica]
          Length = 573

 Score =  314 bits (804), Expect = 3e-83
 Identities = 154/220 (70%), Positives = 182/220 (82%), Gaps = 2/220 (0%)
 Frame = -3

Query: 655 TMISVETQKPDVVLXXXXXXXXXTSSN--HVFKSKLPDIPISNHLPLHTYCFEKLSEFPD 482
           ++ SVETQ  ++            SS   HVF SKLP IPISNHLPLHTYCF  L+++P+
Sbjct: 3   SVASVETQNSELSSLASPQKPQTQSSEETHVFISKLPQIPISNHLPLHTYCFGNLAQYPE 62

Query: 481 RTCLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASM 302
           R CLI GS+ G+TYSF+ETHL C+KTAAGLS LG+KKGDVIM+LLQNCPEFVF+F+GASM
Sbjct: 63  RPCLIVGST-GKTYSFSETHLLCRKTAAGLSKLGLKKGDVIMVLLQNCPEFVFTFMGASM 121

Query: 301 IGAITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFGENGHKLGETYKIITVDD 122
           IGA+TTTANPFYTS+EVFKQF  S AK+IITQSQYVDKL D G+N  KLGE + +IT+DD
Sbjct: 122 IGAVTTTANPFYTSSEVFKQFGASEAKMIITQSQYVDKLRDPGQNNPKLGEDFTVITIDD 181

Query: 121 PPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTG 2
           PP+NCLHFS++S+ANE +IPSV IDPNDPVALPFSSGTTG
Sbjct: 182 PPENCLHFSVLSEANENDIPSVSIDPNDPVALPFSSGTTG 221


>ref|XP_007029575.1| 4-coumarate:CoA ligase 3 [Theobroma cacao]
           gi|508718180|gb|EOY10077.1| 4-coumarate:CoA ligase 3
           [Theobroma cacao]
          Length = 575

 Score =  310 bits (793), Expect = 5e-82
 Identities = 153/219 (69%), Positives = 182/219 (83%), Gaps = 5/219 (2%)
 Frame = -3

Query: 643 VETQKPDV---VLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTC 473
           VE QKP++   +          T+ +H+F+SKLPDIPISNHLPLHTYCFE LS FPD+ C
Sbjct: 8   VEPQKPELSPSLSPPATKPSSETTGDHIFRSKLPDIPISNHLPLHTYCFENLSSFPDKPC 67

Query: 472 LISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGA 293
           LISGSS G+TYSF+ETHL  +KTAAGLSNLGI+KGDVIMILLQNC EFVFSF+GASMIGA
Sbjct: 68  LISGSS-GKTYSFSETHLIAQKTAAGLSNLGIRKGDVIMILLQNCAEFVFSFMGASMIGA 126

Query: 292 ITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDF--GENGHKLGETYKIITVDDP 119
           ++TTANPFYTS E+FKQ+  + AKLI+TQSQYVDKL D    EN  K+GE +K+IT+DDP
Sbjct: 127 VSTTANPFYTSTEIFKQYKVAQAKLIVTQSQYVDKLKDTSNNENFPKMGEDFKVITIDDP 186

Query: 118 PKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTG 2
           P+NCLHF+++S+ANE EIP V IDP+DPVALPFSSGTTG
Sbjct: 187 PENCLHFTVLSEANENEIPQVSIDPDDPVALPFSSGTTG 225


>gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]
          Length = 565

 Score =  303 bits (777), Expect = 4e-80
 Identities = 148/192 (77%), Positives = 169/192 (88%)
 Frame = -3

Query: 577 NHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCLISGSSNGRTYSFAETHLACKKTAA 398
           N +F+SKLPDIPISNHLPLHTYCFEK++EF D  CLISGSS GR Y+FAETHL C+K+AA
Sbjct: 27  NFIFRSKLPDIPISNHLPLHTYCFEKITEFSDAPCLISGSS-GRIYTFAETHLICQKSAA 85

Query: 397 GLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAITTTANPFYTSAEVFKQFTTSNAKL 218
           GLS LGIKKGDVIMILLQN PEF FSF+GASMIGAITTTANPF TSAE+FKQF+ S AKL
Sbjct: 86  GLSKLGIKKGDVIMILLQNSPEFAFSFMGASMIGAITTTANPFCTSAEIFKQFSVSGAKL 145

Query: 217 IITQSQYVDKLVDFGENGHKLGETYKIITVDDPPKNCLHFSIISDANETEIPSVFIDPND 38
           +ITQSQYVDKL D GE   K+GE + +ITVDDPP++CLHFS+IS A+E EIPSV IDP+D
Sbjct: 146 VITQSQYVDKLRDTGEKFPKIGEDFTVITVDDPPESCLHFSVISGADEREIPSVSIDPDD 205

Query: 37  PVALPFSSGTTG 2
           PV++PFSSGTTG
Sbjct: 206 PVSMPFSSGTTG 217


>gb|AGO89321.1| Ca4CL4 [Salix arbutifolia]
          Length = 559

 Score =  303 bits (776), Expect = 5e-80
 Identities = 148/220 (67%), Positives = 179/220 (81%), Gaps = 2/220 (0%)
 Frame = -3

Query: 655 TMISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRT 476
           ++ +VE  KP++             ++HVF+SKLPDIPISNHLPLHTYCFE LS+F DR 
Sbjct: 3   SVATVEPTKPELSPPQSQNAPSSDETDHVFRSKLPDIPISNHLPLHTYCFENLSDFSDRP 62

Query: 475 CLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIG 296
           CLISGS+ G+TYSFAETHL  +K AAGLSNLGIKKGDVIM LLQNCPEF+FSF+GASMIG
Sbjct: 63  CLISGST-GKTYSFAETHLVSRKVAAGLSNLGIKKGDVIMTLLQNCPEFIFSFVGASMIG 121

Query: 295 AITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFG--ENGHKLGETYKIITVDD 122
           A+TTTANPFYT +E+FKQF+ S AKLIITQSQYV KL D    EN    GE + +IT+DD
Sbjct: 122 AVTTTANPFYTPSEIFKQFSASRAKLIITQSQYVTKLRDSDCCENSKNPGEDFVVITIDD 181

Query: 121 PPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTG 2
           PP+NCLHF+++++ANE+E+P+V I P+DPVALPFSSGTTG
Sbjct: 182 PPENCLHFTVLAEANESEMPTVSIHPDDPVALPFSSGTTG 221


>gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]
          Length = 604

 Score =  297 bits (761), Expect = 3e-78
 Identities = 147/202 (72%), Positives = 170/202 (84%), Gaps = 8/202 (3%)
 Frame = -3

Query: 583 SSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCLISGSSNGRTYSFAETHLACKKT 404
           ++NHVFKSKLPDIPI NHLPLHTYCF+KLSEF DR CLI  SS G++YSF++T+L  +KT
Sbjct: 55  TTNHVFKSKLPDIPIPNHLPLHTYCFQKLSEFSDRPCLIV-SSTGKSYSFSDTYLISQKT 113

Query: 403 AAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAITTTANPFYTSAEVFKQFTTSNA 224
            AGLSNLGIKKGDVIMILLQNC EFVF+F+GASMIGAITTTANPFYT+ E+FKQF  SNA
Sbjct: 114 GAGLSNLGIKKGDVIMILLQNCAEFVFAFMGASMIGAITTTANPFYTATEIFKQFKNSNA 173

Query: 223 KLIITQSQYVDKLVDFGENGH--------KLGETYKIITVDDPPKNCLHFSIISDANETE 68
           KLIIT SQ+VDKL +   +G         KLGE +K++TVDDPP+NCLHFS+IS+ANE E
Sbjct: 174 KLIITHSQFVDKLREAPADGAAPDQNNYLKLGEHFKVVTVDDPPENCLHFSVISEANEKE 233

Query: 67  IPSVFIDPNDPVALPFSSGTTG 2
           +P V IDP DPVALPFSSGTTG
Sbjct: 234 LPEVVIDPEDPVALPFSSGTTG 255


>gb|AFC89538.1| 4-coumarate: coenzyme A ligase 2 [Populus tomentosa]
          Length = 569

 Score =  295 bits (755), Expect = 1e-77
 Identities = 145/220 (65%), Positives = 177/220 (80%), Gaps = 2/220 (0%)
 Frame = -3

Query: 655 TMISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRT 476
           ++ +VE  KP++             ++H+F+SKLPDI ISNHLPLH YCFE LS+F DR 
Sbjct: 2   SVATVEPPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENLSDFSDRP 61

Query: 475 CLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIG 296
           CLISGS+ G+TYSFAETHL  +K AAGLSNLGIKKGDVIM LLQNCPEFVFSF+GASMIG
Sbjct: 62  CLISGST-GKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMGASMIG 120

Query: 295 AITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKL--VDFGENGHKLGETYKIITVDD 122
           A+TTTANPFYT +E+FKQF+ S AKLIITQSQYV+KL   D  EN  K GE + +IT+DD
Sbjct: 121 AVTTTANPFYTQSEIFKQFSASRAKLIITQSQYVNKLGDSDCHENNQKPGEDFIVITIDD 180

Query: 121 PPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTG 2
           PP+NC HF+++ +ANE+E+P++ I P+DPVALPFSSGTTG
Sbjct: 181 PPENCRHFNVLVEANESEMPTISILPDDPVALPFSSGTTG 220


>dbj|BAG82851.1| 4-coumarate:CoA ligase [Ipomoea batatas]
          Length = 569

 Score =  295 bits (755), Expect = 1e-77
 Identities = 140/218 (64%), Positives = 178/218 (81%), Gaps = 1/218 (0%)
 Frame = -3

Query: 652 MISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTC 473
           M+SVETQKP++ +         +S  H+F+S+LPDIPI N +PLHTYCF+ L+E+ DRTC
Sbjct: 1   MLSVETQKPELEVADSGKTQAQSSQIHIFRSRLPDIPIPNQIPLHTYCFQNLAEYRDRTC 60

Query: 472 LISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGA 293
           LI GS+ G+TYSFAETHL C+K A+GL+ LG+KKGDVIM L QNCPEFVFSF+GASMIGA
Sbjct: 61  LIVGST-GKTYSFAETHLICRKVASGLAKLGVKKGDVIMTLFQNCPEFVFSFMGASMIGA 119

Query: 292 ITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFGENGHKLGETYKIITVDDPPK 113
           +TTTANPFYT AE+FKQ + S AK++ITQS YVDKL D GE   K+GE + ++T+DDPP+
Sbjct: 120 VTTTANPFYTKAEIFKQMSASKAKVVITQSVYVDKLKDSGEEYPKIGEDFSVVTIDDPPE 179

Query: 112 NCLHFSIISDANETEIP-SVFIDPNDPVALPFSSGTTG 2
           NCLHFS++S+A+E ++P +V I P+D VALPFSSGTTG
Sbjct: 180 NCLHFSVLSEADEEDMPATVEIAPDDAVALPFSSGTTG 217


>gb|AAK58909.1| 4-coumarate:CoA ligase 4 [Populus trichocarpa x Populus deltoides]
          Length = 579

 Score =  293 bits (751), Expect = 4e-77
 Identities = 144/220 (65%), Positives = 176/220 (80%), Gaps = 2/220 (0%)
 Frame = -3

Query: 655 TMISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRT 476
           ++ +VE  KP++             ++H+F+SKLPDI ISNHLPLH YCFE LS+F DR 
Sbjct: 3   SVATVEPPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENLSDFSDRP 62

Query: 475 CLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIG 296
           CLISGS+ G+TYSFAETHL  +K AAGLSNLGIKKGDVIM LLQNCPEFVFSF+GASMIG
Sbjct: 63  CLISGST-GKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMGASMIG 121

Query: 295 AITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKL--VDFGENGHKLGETYKIITVDD 122
           A+TTT NPFYT  E+FKQF+ S AKLIITQSQ+V+KL   D+ EN  K  E + +IT+DD
Sbjct: 122 AVTTTVNPFYTPGEIFKQFSASRAKLIITQSQHVNKLRDSDYHENNQKPEEDFIVITIDD 181

Query: 121 PPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTG 2
           PP+NCLHF+++ +ANE+E+P+V I P+DPVALPFSSGTTG
Sbjct: 182 PPENCLHFNVLVEANESEMPTVSIHPDDPVALPFSSGTTG 221


>gb|ABA40922.1| 4-coumaroyl CoA ligase [Camellia sinensis]
          Length = 588

 Score =  292 bits (748), Expect = 9e-77
 Identities = 143/215 (66%), Positives = 173/215 (80%)
 Frame = -3

Query: 646 SVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCLI 467
           S ET KP+            +S  HVF+SKLP IPISNHLPLHTY F+ LS+FP+R CL+
Sbjct: 6   SAETPKPEFSPPPPQNPQSSSSQTHVFRSKLPHIPISNHLPLHTYRFQNLSQFPNRPCLL 65

Query: 466 SGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAIT 287
            GS+ G++YSF+ THL  +K A+GLS LGIKKGDVIM+LLQNC EFVF+F+GASMIGA+T
Sbjct: 66  IGST-GKSYSFSXTHLVSRKVASGLSLLGIKKGDVIMLLLQNCAEFVFAFMGASMIGAVT 124

Query: 286 TTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFGENGHKLGETYKIITVDDPPKNC 107
           TTANPFYTSAEVFKQF  S +KLIIT SQYVDKL D G+N     + + +IT+DDPP+NC
Sbjct: 125 TTANPFYTSAEVFKQFNASKSKLIITHSQYVDKLRDAGDNFPNWAKEFSVITIDDPPENC 184

Query: 106 LHFSIISDANETEIPSVFIDPNDPVALPFSSGTTG 2
           LHFS++S+ANE+EIP+V IDP+DPVALPFSSGTTG
Sbjct: 185 LHFSVLSEANESEIPTVSIDPDDPVALPFSSGTTG 219


>ref|XP_002325815.1| 4-coumarate-CoA ligase family protein [Populus trichocarpa]
           gi|118487236|gb|ABK95446.1| unknown [Populus
           trichocarpa] gi|222862690|gb|EEF00197.1| 4-coumarate-CoA
           ligase family protein [Populus trichocarpa]
          Length = 570

 Score =  291 bits (746), Expect = 1e-76
 Identities = 144/220 (65%), Positives = 175/220 (79%), Gaps = 2/220 (0%)
 Frame = -3

Query: 655 TMISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRT 476
           ++ +VE  KP++             ++H+F+SKLPDI ISNHLPLH YCFE LS+F DR 
Sbjct: 3   SVATVEPPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENLSDFSDRP 62

Query: 475 CLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIG 296
           CLISGS+ G+TYSFAETHL  +K AAGLSNLGIKKGDVIM LLQNCPEFVFSF+GASMIG
Sbjct: 63  CLISGST-GKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMGASMIG 121

Query: 295 AITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKL--VDFGENGHKLGETYKIITVDD 122
           A+TTT NPFYT  E+FKQF+ S AKLIITQSQ+V+KL   D  EN  K  E + +IT+DD
Sbjct: 122 AVTTTVNPFYTPGEIFKQFSASRAKLIITQSQHVNKLRDSDCHENNQKPEEDFIVITIDD 181

Query: 121 PPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTG 2
           PP+NCLHF+++ +ANE+E+P+V I P+DPVALPFSSGTTG
Sbjct: 182 PPENCLHFNVLVEANESEMPTVSIHPDDPVALPFSSGTTG 221


>gb|AAC24504.1| 4-coumarate:CoA ligase [Populus tremuloides]
          Length = 570

 Score =  291 bits (745), Expect = 2e-76
 Identities = 145/220 (65%), Positives = 176/220 (80%), Gaps = 2/220 (0%)
 Frame = -3

Query: 655 TMISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRT 476
           ++ +VE  KP++             ++H+F+SKLPDI ISN LPLH YCFE LS+F DR 
Sbjct: 3   SVATVEPPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNDLPLHAYCFENLSDFSDRP 62

Query: 475 CLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIG 296
           CLISGS+ G+TYSFAETHL  +K AAGLSNLGIKKGDVIM LLQNCPEFVFSFIGASMIG
Sbjct: 63  CLISGST-GKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFIGASMIG 121

Query: 295 AITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKL--VDFGENGHKLGETYKIITVDD 122
           A+ TTANPFYT +E+FKQF+ S AKLIITQSQYV+KL   D  EN  K GE + +IT+DD
Sbjct: 122 AVITTANPFYTQSEIFKQFSASRAKLIITQSQYVNKLGDSDCHENNQKPGEDFIVITIDD 181

Query: 121 PPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTG 2
           PP+NCLHF+++ +A+E+E+P+V I P+DPVALPFSSGTTG
Sbjct: 182 PPENCLHFNVLVEASESEMPTVSILPDDPVALPFSSGTTG 221


>gb|AEX32786.1| coumaroyl-CoA ligase [Vitis vinifera]
          Length = 570

 Score =  290 bits (743), Expect = 3e-76
 Identities = 139/221 (62%), Positives = 177/221 (80%), Gaps = 4/221 (1%)
 Frame = -3

Query: 652 MISVETQKPDVVLXXXXXXXXXTSS---NHVFKSKLPDIPISNHLPLHTYCFEKLSEFPD 482
           MIS+ETQ PDV            ++   +HVF+SKLP+IPISNHLPLHTYCFE  S+F D
Sbjct: 1   MISIETQNPDVSNLDTSHSIPKMANRIDDHVFRSKLPEIPISNHLPLHTYCFENYSQFAD 60

Query: 481 RTCLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASM 302
           R CLI GS+N +TYSFAETHL  +K  AG ++LG+K+GDV+MILLQNC EF FSF+GASM
Sbjct: 61  RPCLIVGSTN-KTYSFAETHLISRKVGAGFAHLGLKQGDVVMILLQNCAEFAFSFLGASM 119

Query: 301 IGAITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFGENG-HKLGETYKIITVD 125
           +GA+TTTANPFYTSAE+FKQ   S AK+++TQ+QYVDKL D+ +    K+GE + +IT+D
Sbjct: 120 VGAVTTTANPFYTSAEIFKQLNASKAKIVVTQAQYVDKLRDYPDGQVAKIGEGFTVITID 179

Query: 124 DPPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTG 2
           DPP+NC+HFS++S+ANE+E+P V I+ +DPVALPFSSGTTG
Sbjct: 180 DPPENCMHFSVVSEANESELPEVSINSDDPVALPFSSGTTG 220


>ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis vinifera]
          Length = 570

 Score =  290 bits (743), Expect = 3e-76
 Identities = 139/221 (62%), Positives = 177/221 (80%), Gaps = 4/221 (1%)
 Frame = -3

Query: 652 MISVETQKPDVVLXXXXXXXXXTSS---NHVFKSKLPDIPISNHLPLHTYCFEKLSEFPD 482
           MIS+ETQ PDV            ++   +HVF+SKLP+IPISNHLPLHTYCFE  S+F D
Sbjct: 1   MISIETQNPDVSNLDTSHSIPKMANRIDDHVFRSKLPEIPISNHLPLHTYCFENYSQFAD 60

Query: 481 RTCLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASM 302
           R CLI GS+N +TYSFAETHL  +K  AG ++LG+K+GDV+MILLQNC EF FSF+GASM
Sbjct: 61  RPCLIVGSTN-KTYSFAETHLISRKVGAGFAHLGLKQGDVVMILLQNCAEFAFSFLGASM 119

Query: 301 IGAITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFGENG-HKLGETYKIITVD 125
           +GA+TTTANPFYTSAE+FKQ   S AK+++TQ+QYVDKL D+ +    K+GE + +IT+D
Sbjct: 120 VGAVTTTANPFYTSAEIFKQLNASKAKIVVTQAQYVDKLRDYPDGQVAKIGEGFTVITID 179

Query: 124 DPPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTG 2
           DPP+NC+HFS++S+ANE+E+P V I+ +DPVALPFSSGTTG
Sbjct: 180 DPPENCMHFSVVSEANESELPEVSINSDDPVALPFSSGTTG 220


>gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum]
          Length = 573

 Score =  289 bits (740), Expect = 7e-76
 Identities = 146/221 (66%), Positives = 174/221 (78%), Gaps = 5/221 (2%)
 Frame = -3

Query: 649 ISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCL 470
           + +E+ KPD +          T ++H+F+SKLPDIPISNHLPLHTYCFE L    D+ CL
Sbjct: 9   VELESPKPDQL-------SPETPADHIFRSKLPDIPISNHLPLHTYCFENLPSLSDKPCL 61

Query: 469 ISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAI 290
           ISGSS G+ YSF+ETHL  +KTAAGLSNLGI+KGD IMILL NC EFVF+F+GASMIGA+
Sbjct: 62  ISGSS-GKIYSFSETHLIAQKTAAGLSNLGIQKGDAIMILLHNCAEFVFAFMGASMIGAV 120

Query: 289 TTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFG-----ENGHKLGETYKIITVD 125
           +TTANPFYTS E+FKQF  + AKLIITQSQYVDKL D       EN  K+GE +K+IT+D
Sbjct: 121 STTANPFYTSKEIFKQFKAARAKLIITQSQYVDKLKDTNNNDGDENFPKIGEDFKVITID 180

Query: 124 DPPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTG 2
           DPP+NCL FS++S+ANE EIP V I P+DPVALPFSSGTTG
Sbjct: 181 DPPENCLDFSMLSEANENEIPQVTIHPDDPVALPFSSGTTG 221


>gb|AFG30056.1| 4-coumarate: CoA ligase [Malus hybrid cultivar]
          Length = 605

 Score =  289 bits (739), Expect = 1e-75
 Identities = 141/200 (70%), Positives = 165/200 (82%), Gaps = 8/200 (4%)
 Frame = -3

Query: 577 NHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCLISGSSNGRTYSFAETHLACKKTAA 398
           NHVF+SKLPDIPI NHLPLHTYCF+ L EF D+ CLI GS+ G++YSF+ETHL  +KT A
Sbjct: 57  NHVFRSKLPDIPIPNHLPLHTYCFQNLPEFSDKPCLIVGST-GKSYSFSETHLISQKTGA 115

Query: 397 GLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAITTTANPFYTSAEVFKQFTTSNAKL 218
           GLSNLGI+KGDVIMILLQNC EFVF+F+GAS+IGA+TTTANPFYTSAE+FKQ   +NAKL
Sbjct: 116 GLSNLGIQKGDVIMILLQNCAEFVFAFMGASLIGAVTTTANPFYTSAEIFKQVKAANAKL 175

Query: 217 IITQSQYVDKLVDF--------GENGHKLGETYKIITVDDPPKNCLHFSIISDANETEIP 62
           IITQSQYVDKL +           N  KLGE +K++T+DDPP+NCLHFS+ S+ANE E+P
Sbjct: 176 IITQSQYVDKLREHPSSADGADQNNYPKLGEDFKVVTIDDPPENCLHFSVFSEANEKELP 235

Query: 61  SVFIDPNDPVALPFSSGTTG 2
            V ID  DPVALPFSSGTTG
Sbjct: 236 DVVIDAEDPVALPFSSGTTG 255


>gb|EYU45376.1| hypothetical protein MIMGU_mgv1a003734mg [Mimulus guttatus]
          Length = 567

 Score =  287 bits (735), Expect = 3e-75
 Identities = 134/219 (61%), Positives = 172/219 (78%), Gaps = 1/219 (0%)
 Frame = -3

Query: 655 TMISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRT 476
           ++ S ETQ P++              +HVFKSKLP IPISNHLPLHTYCFE  S +PDR 
Sbjct: 3   SVASAETQNPELSSPIITQKQSSPEKSHVFKSKLPTIPISNHLPLHTYCFENFSHYPDRK 62

Query: 475 CLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIG 296
           CL+ G+  G++Y++AETHL C+K AAGLSNLGIKKGDV+++LLQNC EFVF+F+GASMIG
Sbjct: 63  CLLVGNGTGKSYTYAETHLICRKVAAGLSNLGIKKGDVVLVLLQNCAEFVFTFMGASMIG 122

Query: 295 AITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFGENGHKLGETYKIITVDDPP 116
           A+TT ANPF T+ E+FKQF  SN+++IITQ+QYVDKL + G++  K GE + +ITVDDPP
Sbjct: 123 AVTTAANPFCTANEIFKQFRASNSRMIITQAQYVDKLREGGDDSPKFGEDFSVITVDDPP 182

Query: 115 KNCLHFSIISDANETEIP-SVFIDPNDPVALPFSSGTTG 2
           + CLHFS++S+ANE + P +V ID +D V+LPFSSGTTG
Sbjct: 183 EGCLHFSVLSEANENDAPAAVEIDSDDAVSLPFSSGTTG 221


>gb|ACZ06243.1| 4-coumarate:CoA ligase 2 [Gossypium hirsutum]
          Length = 574

 Score =  287 bits (735), Expect = 3e-75
 Identities = 145/222 (65%), Positives = 173/222 (77%), Gaps = 6/222 (2%)
 Frame = -3

Query: 649 ISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCL 470
           + +E+ KPD +          T ++H+F+SKLPDIPISNHLPLHTYCFE L    D+ CL
Sbjct: 9   VELESPKPDQL-------SPETPADHIFRSKLPDIPISNHLPLHTYCFENLPSLSDKPCL 61

Query: 469 ISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAI 290
           ISGSS G+ YSF+ETHL  +KTAAGLS LGI+KGD IMILL NC EFVF+F+GASMIGA+
Sbjct: 62  ISGSS-GKIYSFSETHLIAQKTAAGLSKLGIQKGDAIMILLHNCAEFVFAFMGASMIGAV 120

Query: 289 TTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFG------ENGHKLGETYKIITV 128
           +TTANPFYTS E+FKQF  + AKLIITQSQYVDKL D        EN  K+GE +K+IT+
Sbjct: 121 STTANPFYTSKEIFKQFKAARAKLIITQSQYVDKLKDTNNNNDGVENFPKIGEDFKVITI 180

Query: 127 DDPPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTG 2
           DDPP+NCL FS++S+ANE EIP V I P+DPVALPFSSGTTG
Sbjct: 181 DDPPENCLDFSVLSEANENEIPQVTIHPDDPVALPFSSGTTG 222


>ref|XP_004307864.1| PREDICTED: LOW QUALITY PROTEIN: 4-coumarate--CoA ligase 2-like
           [Fragaria vesca subsp. vesca]
          Length = 586

 Score =  287 bits (734), Expect = 4e-75
 Identities = 141/196 (71%), Positives = 167/196 (85%), Gaps = 4/196 (2%)
 Frame = -3

Query: 577 NHVFKSKLPDIP-ISNHLPLHTYCFEKLSEFPDRTCLISGSSNGRTYSFAETHLACKKTA 401
           +HVFKSKLPD+P ISNHLPLHTYCFE +S+F +R CLI GS+ G+TYSF+ETHL  +KT 
Sbjct: 43  HHVFKSKLPDLPNISNHLPLHTYCFENVSDFSERPCLIVGST-GKTYSFSETHLISQKTG 101

Query: 400 AGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAITTTANPFYTSAEVFKQFTTSNAK 221
           AGLS LGI KGDVIMILLQNC EFVF+F+GASMIGA+TTTANPFYT++E+FKQF  SNAK
Sbjct: 102 AGLSKLGITKGDVIMILLQNCAEFVFAFMGASMIGAVTTTANPFYTASEIFKQFKASNAK 161

Query: 220 LIITQSQYVDKLVDFGENGH---KLGETYKIITVDDPPKNCLHFSIISDANETEIPSVFI 50
           LIITQS YVDKL   G +     KLGE ++++T+DDPP+NCLHFS++S ANE+E+P V I
Sbjct: 162 LIITQSIYVDKLRHSGPDSDQYPKLGEHFQVVTIDDPPENCLHFSVLSGANESELPEVSI 221

Query: 49  DPNDPVALPFSSGTTG 2
           DP DPVALPFSSGTTG
Sbjct: 222 DPEDPVALPFSSGTTG 237


>gb|ADE96997.1| p-coumarate:CoA-ligase 3 [Sorbus aucuparia]
          Length = 605

 Score =  285 bits (728), Expect = 2e-74
 Identities = 139/200 (69%), Positives = 165/200 (82%), Gaps = 8/200 (4%)
 Frame = -3

Query: 577 NHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCLISGSSNGRTYSFAETHLACKKTAA 398
           NHVF+SKLPDI I NHLPLHTYCF+ L EF DR CLI GS+ G++YSF+ETHL  +KT A
Sbjct: 57  NHVFRSKLPDIAIPNHLPLHTYCFQNLPEFSDRPCLIVGST-GKSYSFSETHLISQKTGA 115

Query: 397 GLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAITTTANPFYTSAEVFKQFTTSNAKL 218
           GLSNLGI+KGDVIMILLQNC EFVF+F+GAS+IGA+TTTANPFYT+AE+FKQ   +NAKL
Sbjct: 116 GLSNLGIQKGDVIMILLQNCAEFVFAFMGASLIGAVTTTANPFYTTAEIFKQVKAANAKL 175

Query: 217 IITQSQYVDKLVDF--------GENGHKLGETYKIITVDDPPKNCLHFSIISDANETEIP 62
           IITQSQYV+KL +           N  KLGE +K++T+DDPP+NCLHFS++S+ANE E+P
Sbjct: 176 IITQSQYVNKLREHPSSADGADQNNYPKLGEDFKVVTIDDPPENCLHFSVLSEANEKELP 235

Query: 61  SVFIDPNDPVALPFSSGTTG 2
            V ID  DPVALPFSSGTTG
Sbjct: 236 DVVIDAEDPVALPFSSGTTG 255


>gb|ADU15550.1| 4CL [Gossypium hirsutum]
          Length = 574

 Score =  283 bits (725), Expect = 4e-74
 Identities = 144/222 (64%), Positives = 171/222 (77%), Gaps = 6/222 (2%)
 Frame = -3

Query: 649 ISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCL 470
           + +E  KPD +          T ++H+F+SKLPDIPISNHLPLHTYCFE L    D+ CL
Sbjct: 9   VELEPPKPDQL-------SPETPADHIFRSKLPDIPISNHLPLHTYCFENLPSLSDKPCL 61

Query: 469 ISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAI 290
           ISGSS G+ YSF+ETHL  +KTAAGLS LGI+KGD IMILL NC EFVF+F+GASMIGA+
Sbjct: 62  ISGSS-GKIYSFSETHLIAQKTAAGLSKLGIQKGDAIMILLHNCAEFVFAFMGASMIGAV 120

Query: 289 TTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFG------ENGHKLGETYKIITV 128
           +TTANPFYTS E+FKQ   + AKLIITQSQYVDKL D        EN  K+GE +K+IT+
Sbjct: 121 STTANPFYTSKEIFKQSKAARAKLIITQSQYVDKLKDTNNNNDGVENFPKIGEDFKVITI 180

Query: 127 DDPPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTG 2
           DDPP+NCL FS++S+ANE EIP V I P+DPVALPFSSGTTG
Sbjct: 181 DDPPENCLDFSVLSEANENEIPQVTIHPDDPVALPFSSGTTG 222