BLASTX nr result
ID: Paeonia23_contig00038529
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Paeonia23_contig00038529
(494 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_006477927.1| PREDICTED: cationic amino acid transporter 8... 162 4e-38
ref|XP_004251831.1| PREDICTED: cationic amino acid transporter 8... 161 1e-37
ref|XP_007022159.1| Cationic amino acid transporter 8 [Theobroma... 160 2e-37
ref|XP_006349994.1| PREDICTED: cationic amino acid transporter 8... 159 3e-37
ref|XP_007211395.1| hypothetical protein PRUPE_ppa003337mg [Prun... 159 4e-37
ref|XP_002300432.2| cationic amino acid transporter 8 family pro... 154 2e-35
ref|XP_002518795.1| cationic amino acid transporter, putative [R... 152 6e-35
ref|XP_002890204.1| hypothetical protein ARALYDRAFT_471910 [Arab... 150 1e-34
ref|XP_004301950.1| PREDICTED: cationic amino acid transporter 8... 150 2e-34
ref|XP_006307083.1| hypothetical protein CARUB_v10008669mg [Caps... 150 2e-34
ref|NP_173155.1| cationic amino acid transporter 8 [Arabidopsis ... 149 4e-34
gb|EYU45055.1| hypothetical protein MIMGU_mgv1a003403mg [Mimulus... 149 5e-34
ref|XP_006416741.1| hypothetical protein EUTSA_v10007175mg [Eutr... 147 1e-33
ref|XP_002278606.1| PREDICTED: uncharacterized amino acid permea... 147 1e-33
emb|CAN78727.1| hypothetical protein VITISV_020011 [Vitis vinifera] 147 1e-33
emb|CBI27075.3| unnamed protein product [Vitis vinifera] 146 3e-33
ref|XP_004164930.1| PREDICTED: cationic amino acid transporter 8... 144 2e-32
ref|XP_004137314.1| PREDICTED: cationic amino acid transporter 8... 144 2e-32
emb|CBI33021.3| unnamed protein product [Vitis vinifera] 140 2e-31
gb|EXB52061.1| Cationic amino acid transporter 8 [Morus notabilis] 137 2e-30
>ref|XP_006477927.1| PREDICTED: cationic amino acid transporter 8, vacuolar-like [Citrus
sinensis]
Length = 618
Score = 162 bits (410), Expect = 4e-38
Identities = 74/104 (71%), Positives = 88/104 (84%)
Frame = +2
Query: 2 WNSKYGRWWIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGS 181
WNS GR WIGYV+ W +GTLGM L+PKQR PK+WGVPLVPW+P+LSI MN+FLIGS
Sbjct: 515 WNSN-GRGWIGYVVTIAPWFLGTLGMALLPKQRVPKLWGVPLVPWLPSLSIAMNLFLIGS 573
Query: 182 LGYVAFLRFFICSAVILVYYMLFGLHATYDVAHQDERESEIEQG 313
LGY+AFLRF ICSAV+LVYY+L GLHATYDVAHQ++ +S E+G
Sbjct: 574 LGYLAFLRFIICSAVMLVYYLLVGLHATYDVAHQNQEKSNNEEG 617
>ref|XP_004251831.1| PREDICTED: cationic amino acid transporter 8, vacuolar-like
[Solanum lycopersicum]
Length = 617
Score = 161 bits (407), Expect = 1e-37
Identities = 68/102 (66%), Positives = 89/102 (87%)
Frame = +2
Query: 26 WIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGSLGYVAFLR 205
W+GY + G+ W++GT GM L+PKQR PKVWGVPLVPW+P+LSIGMNIFLIGSLG AF R
Sbjct: 514 WVGYAVTGVLWLLGTSGMALLPKQRIPKVWGVPLVPWVPSLSIGMNIFLIGSLGKEAFYR 573
Query: 206 FFICSAVILVYYMLFGLHATYDVAHQDERESEIEQGKENINQ 331
FFICSAV+L+YY+L G+HATYD+AH D+++S I++GK++ +Q
Sbjct: 574 FFICSAVMLIYYVLVGVHATYDIAHPDKQKSLIDEGKDSSDQ 615
>ref|XP_007022159.1| Cationic amino acid transporter 8 [Theobroma cacao]
gi|508721787|gb|EOY13684.1| Cationic amino acid
transporter 8 [Theobroma cacao]
Length = 693
Score = 160 bits (404), Expect = 2e-37
Identities = 73/103 (70%), Positives = 87/103 (84%)
Frame = +2
Query: 2 WNSKYGRWWIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGS 181
WNS + R WIGY +AG+ W +GTLGM + K R PKVWGVPLVPW+P+LSI MN+FLIGS
Sbjct: 477 WNS-HERGWIGYTVAGLLWFLGTLGMVFLSKHRVPKVWGVPLVPWLPSLSIVMNLFLIGS 535
Query: 182 LGYVAFLRFFICSAVILVYYMLFGLHATYDVAHQDERESEIEQ 310
LG+VAFLRF ICSAV+LVYY+L GLHATYDVAHQ+E S+IE+
Sbjct: 536 LGFVAFLRFIICSAVMLVYYLLVGLHATYDVAHQNEEVSKIEE 578
>ref|XP_006349994.1| PREDICTED: cationic amino acid transporter 8, vacuolar-like
[Solanum tuberosum]
Length = 618
Score = 159 bits (403), Expect = 3e-37
Identities = 68/102 (66%), Positives = 87/102 (85%)
Frame = +2
Query: 26 WIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGSLGYVAFLR 205
WIGY + G+ W++GT GM L+PKQR PKVWGVPLVPW+P+LSIGMNIFLIGSLG AF R
Sbjct: 515 WIGYAVTGVLWLLGTSGMALLPKQRIPKVWGVPLVPWVPSLSIGMNIFLIGSLGKAAFYR 574
Query: 206 FFICSAVILVYYMLFGLHATYDVAHQDERESEIEQGKENINQ 331
FFICS V+L+YY+L G+HATYD+AH D+++S I++GK + +Q
Sbjct: 575 FFICSVVMLIYYVLVGVHATYDIAHPDKQKSVIDEGKGSSDQ 616
>ref|XP_007211395.1| hypothetical protein PRUPE_ppa003337mg [Prunus persica]
gi|462407260|gb|EMJ12594.1| hypothetical protein
PRUPE_ppa003337mg [Prunus persica]
Length = 584
Score = 159 bits (402), Expect = 4e-37
Identities = 71/107 (66%), Positives = 88/107 (82%)
Frame = +2
Query: 2 WNSKYGRWWIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGS 181
WN+ R WI YV+ G+ W++GTL M +PK R PKVWGVPLVPW PALSIG+N+FLIGS
Sbjct: 477 WNADK-RGWIWYVVFGVIWLLGTLWMAFLPKHRVPKVWGVPLVPWFPALSIGINVFLIGS 535
Query: 182 LGYVAFLRFFICSAVILVYYMLFGLHATYDVAHQDERESEIEQGKEN 322
LGYVAFLRFFIC+AV++ YY+L G+HATYDVAH E+ES+ E+G E+
Sbjct: 536 LGYVAFLRFFICTAVMVAYYLLVGVHATYDVAHHIEQESKTEEGNES 582
>ref|XP_002300432.2| cationic amino acid transporter 8 family protein [Populus
trichocarpa] gi|550349203|gb|EEE85237.2| cationic amino
acid transporter 8 family protein [Populus trichocarpa]
Length = 604
Score = 154 bits (388), Expect = 2e-35
Identities = 67/105 (63%), Positives = 86/105 (81%)
Frame = +2
Query: 2 WNSKYGRWWIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGS 181
WNS R WIGYV+A + W GTLG+ L+ K R PKVWGVPLVPW+P+LSI MN+FL+GS
Sbjct: 501 WNSGV-RGWIGYVVASVIWCSGTLGISLLHKHRVPKVWGVPLVPWLPSLSIAMNVFLLGS 559
Query: 182 LGYVAFLRFFICSAVILVYYMLFGLHATYDVAHQDERESEIEQGK 316
L Y AFLRF ICSAV+++YY++ G+HATYDVAHQ+ +E+E E+G+
Sbjct: 560 LDYEAFLRFIICSAVMILYYLMIGVHATYDVAHQNPKETEAEEGR 604
>ref|XP_002518795.1| cationic amino acid transporter, putative [Ricinus communis]
gi|223542176|gb|EEF43720.1| cationic amino acid
transporter, putative [Ricinus communis]
Length = 575
Score = 152 bits (383), Expect = 6e-35
Identities = 64/97 (65%), Positives = 82/97 (84%)
Frame = +2
Query: 17 GRWWIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGSLGYVA 196
GR WIGYV+ + W +GTLGM L+PKQ P+VWGVPLVPW+P+LSI MN+FLIGSLG VA
Sbjct: 477 GRGWIGYVVTSVLWFLGTLGMALLPKQHVPRVWGVPLVPWLPSLSIAMNLFLIGSLGVVA 536
Query: 197 FLRFFICSAVILVYYMLFGLHATYDVAHQDERESEIE 307
F RF ICSAV+++YY+ G+HATYD+AHQ++ E++IE
Sbjct: 537 FWRFIICSAVMILYYLFVGVHATYDLAHQNQEETKIE 573
>ref|XP_002890204.1| hypothetical protein ARALYDRAFT_471910 [Arabidopsis lyrata subsp.
lyrata] gi|297336046|gb|EFH66463.1| hypothetical protein
ARALYDRAFT_471910 [Arabidopsis lyrata subsp. lyrata]
Length = 585
Score = 150 bits (380), Expect = 1e-34
Identities = 67/102 (65%), Positives = 80/102 (78%)
Frame = +2
Query: 2 WNSKYGRWWIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGS 181
WN+ G W I Y + G+ W IGTLG+ L+PK R PKVWGVPLVPW+P+ SI MN+FLIGS
Sbjct: 482 WNAGVGGW-IAYAVTGVLWFIGTLGLALLPKYRVPKVWGVPLVPWLPSFSIAMNLFLIGS 540
Query: 182 LGYVAFLRFFICSAVILVYYMLFGLHATYDVAHQDERESEIE 307
LGYVAFLRF IC+ V+L+YY+ GLHATYDVAHQ ES+ E
Sbjct: 541 LGYVAFLRFIICTMVMLLYYLFVGLHATYDVAHQPLEESKFE 582
>ref|XP_004301950.1| PREDICTED: cationic amino acid transporter 8, vacuolar-like
[Fragaria vesca subsp. vesca]
Length = 587
Score = 150 bits (379), Expect = 2e-34
Identities = 68/105 (64%), Positives = 87/105 (82%), Gaps = 1/105 (0%)
Frame = +2
Query: 2 WNSKYGRWWIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGS 181
WN+ R W+ YV+AG+ W+ GTL M L+PK+R KVW VPLVPW+PALSIG+N+FLIGS
Sbjct: 476 WNTNV-RGWVWYVVAGVIWLSGTLWMALLPKRRVAKVWSVPLVPWLPALSIGINLFLIGS 534
Query: 182 LGYVAFLRFFICSAVILVYYMLFGLHATYDVAHQ-DERESEIEQG 313
LGYVAFLRF IC+AV++VYY+ G+HATYDVAHQ D+++S E+G
Sbjct: 535 LGYVAFLRFAICTAVMIVYYLFVGVHATYDVAHQIDQQQSRTEEG 579
>ref|XP_006307083.1| hypothetical protein CARUB_v10008669mg [Capsella rubella]
gi|482575794|gb|EOA39981.1| hypothetical protein
CARUB_v10008669mg [Capsella rubella]
Length = 586
Score = 150 bits (378), Expect = 2e-34
Identities = 66/102 (64%), Positives = 80/102 (78%)
Frame = +2
Query: 2 WNSKYGRWWIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGS 181
WN+ R WI Y + G+ W IGTLG+ L+PK R PKVWGVPLVPW+P+ SI MN+FLIGS
Sbjct: 483 WNAGV-RSWIAYAVTGVIWFIGTLGLALLPKSRVPKVWGVPLVPWLPSFSIAMNLFLIGS 541
Query: 182 LGYVAFLRFFICSAVILVYYMLFGLHATYDVAHQDERESEIE 307
LGYVAFLRF IC+ V+LVYY+ GLHATYDVAHQ +++ E
Sbjct: 542 LGYVAFLRFIICTMVMLVYYLFVGLHATYDVAHQPPEQAKFE 583
>ref|NP_173155.1| cationic amino acid transporter 8 [Arabidopsis thaliana]
gi|75313454|sp|Q9SHH0.1|CAAT8_ARATH RecName:
Full=Cationic amino acid transporter 8, vacuolar
gi|5734765|gb|AAD50030.1|AC007651_25 Very similar to
amino acid transporter [Arabidopsis thaliana]
gi|18176204|gb|AAL60003.1| putative amino acid
transporter protein [Arabidopsis thaliana]
gi|21436167|gb|AAM51371.1| putative amino acid
transporter protein [Arabidopsis thaliana]
gi|332191423|gb|AEE29544.1| cationic amino acid
transporter 8 [Arabidopsis thaliana]
Length = 590
Score = 149 bits (376), Expect = 4e-34
Identities = 66/102 (64%), Positives = 80/102 (78%)
Frame = +2
Query: 2 WNSKYGRWWIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGS 181
WNS + WI Y + G+ W IGTLG+ L+PK R PKVWGVPLVPW+P+ SI MN+FLIGS
Sbjct: 487 WNSGV-KGWIAYTVTGVIWFIGTLGLALLPKYRVPKVWGVPLVPWLPSFSIAMNLFLIGS 545
Query: 182 LGYVAFLRFFICSAVILVYYMLFGLHATYDVAHQDERESEIE 307
LGYVAFLRF IC+ V+L+YY+ GLHATYDVAHQ E++ E
Sbjct: 546 LGYVAFLRFIICTMVMLLYYLFVGLHATYDVAHQPLEEAKFE 587
>gb|EYU45055.1| hypothetical protein MIMGU_mgv1a003403mg [Mimulus guttatus]
Length = 587
Score = 149 bits (375), Expect = 5e-34
Identities = 68/113 (60%), Positives = 89/113 (78%)
Frame = +2
Query: 2 WNSKYGRWWIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGS 181
W+S Y R W+GY ++G W + TLGM L K R P VWGVPLVP +P+LSIGMNIFLIGS
Sbjct: 476 WSSNY-RGWVGYSVSGGLWGLSTLGMGLTSKHRTPAVWGVPLVPLLPSLSIGMNIFLIGS 534
Query: 182 LGYVAFLRFFICSAVILVYYMLFGLHATYDVAHQDERESEIEQGKENINQGAL 340
LG VA RFFICSA+++VYY+L G+HATYDVAH ++ + ++E+G ++N+GAL
Sbjct: 535 LGAVAIWRFFICSAIMMVYYVLAGVHATYDVAHMNQEQVKLEEGDGDLNKGAL 587
>ref|XP_006416741.1| hypothetical protein EUTSA_v10007175mg [Eutrema salsugineum]
gi|557094512|gb|ESQ35094.1| hypothetical protein
EUTSA_v10007175mg [Eutrema salsugineum]
Length = 584
Score = 147 bits (372), Expect = 1e-33
Identities = 65/102 (63%), Positives = 79/102 (77%)
Frame = +2
Query: 2 WNSKYGRWWIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGS 181
WN+ R W+ Y + G+ W IGTLG+ L+PK R PKVWGVPLVPW+P+ SI MN+FLIGS
Sbjct: 481 WNAGV-RGWLAYAVTGVIWFIGTLGLALLPKHRVPKVWGVPLVPWLPSFSIAMNLFLIGS 539
Query: 182 LGYVAFLRFFICSAVILVYYMLFGLHATYDVAHQDERESEIE 307
LGYVAFLRF IC+ V+L+YY+ GLHATYDVAHQ S+ E
Sbjct: 540 LGYVAFLRFIICTMVMLLYYLFVGLHATYDVAHQPMEGSKFE 581
>ref|XP_002278606.1| PREDICTED: uncharacterized amino acid permease YfnA-like [Vitis
vinifera]
Length = 571
Score = 147 bits (371), Expect = 1e-33
Identities = 65/97 (67%), Positives = 78/97 (80%)
Frame = +2
Query: 2 WNSKYGRWWIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGS 181
WNS W +GY + W +GTLGM L+PKQR VWGVPLVPW+P+LSIGMN+FLIGS
Sbjct: 475 WNSNTTEW-VGYAVGAFLWFLGTLGMGLLPKQRVTNVWGVPLVPWLPSLSIGMNLFLIGS 533
Query: 182 LGYVAFLRFFICSAVILVYYMLFGLHATYDVAHQDER 292
LGY AFLRFFICSAV+L+YY L GLHATYD+A Q+++
Sbjct: 534 LGYQAFLRFFICSAVMLIYYFLVGLHATYDMARQNQQ 570
>emb|CAN78727.1| hypothetical protein VITISV_020011 [Vitis vinifera]
Length = 552
Score = 147 bits (371), Expect = 1e-33
Identities = 65/97 (67%), Positives = 78/97 (80%)
Frame = +2
Query: 2 WNSKYGRWWIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGS 181
WNS W +GY + W +GTLGM L+PKQR VWGVPLVPW+P+LSIGMN+FLIGS
Sbjct: 456 WNSNTTEW-VGYAVGAFLWFLGTLGMGLLPKQRVTNVWGVPLVPWLPSLSIGMNLFLIGS 514
Query: 182 LGYVAFLRFFICSAVILVYYMLFGLHATYDVAHQDER 292
LGY AFLRFFICSAV+L+YY L GLHATYD+A Q+++
Sbjct: 515 LGYQAFLRFFICSAVMLIYYFLVGLHATYDMARQNQQ 551
>emb|CBI27075.3| unnamed protein product [Vitis vinifera]
Length = 885
Score = 146 bits (369), Expect = 3e-33
Identities = 68/108 (62%), Positives = 83/108 (76%), Gaps = 1/108 (0%)
Frame = +2
Query: 2 WNSKYGRWWIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGS 181
WNS W +GY + W +GTLGM L+PKQR VWGVPLVPW+P+LSIGMN+FLIGS
Sbjct: 465 WNSNTTEW-VGYAVGAFLWFLGTLGMGLLPKQRVTNVWGVPLVPWLPSLSIGMNLFLIGS 523
Query: 182 LGYVAFLRFFICSAVILVYYMLFGLHATYDVAHQDE-RESEIEQGKEN 322
LGY AFLRFFICSAV+L+YY L GLHATYD+A + E R S + + +E+
Sbjct: 524 LGYQAFLRFFICSAVMLIYYFLVGLHATYDMAPRTESRASNLGKNEED 571
>ref|XP_004164930.1| PREDICTED: cationic amino acid transporter 8, vacuolar-like
[Cucumis sativus]
Length = 630
Score = 144 bits (362), Expect = 2e-32
Identities = 65/99 (65%), Positives = 79/99 (79%)
Frame = +2
Query: 26 WIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGSLGYVAFLR 205
WI YV+ FW + TL M +PK R+PKVWGVPLVPW+P+LSIGMN+ LIGSLG AFLR
Sbjct: 491 WIEYVVPASFWFLSTLAMSFLPKYRSPKVWGVPLVPWLPSLSIGMNLILIGSLGTEAFLR 550
Query: 206 FFICSAVILVYYMLFGLHATYDVAHQDERESEIEQGKEN 322
FFICSAV+L+YY+ GLHATYDVAHQD S+ E+ K++
Sbjct: 551 FFICSAVMLLYYLFIGLHATYDVAHQDGLGSKNEEIKDD 589
>ref|XP_004137314.1| PREDICTED: cationic amino acid transporter 8, vacuolar-like
[Cucumis sativus]
Length = 594
Score = 144 bits (362), Expect = 2e-32
Identities = 65/99 (65%), Positives = 79/99 (79%)
Frame = +2
Query: 26 WIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGSLGYVAFLR 205
WI YV+ FW + TL M +PK R+PKVWGVPLVPW+P+LSIGMN+ LIGSLG AFLR
Sbjct: 491 WIEYVVPASFWFLSTLAMSFLPKYRSPKVWGVPLVPWLPSLSIGMNLILIGSLGTEAFLR 550
Query: 206 FFICSAVILVYYMLFGLHATYDVAHQDERESEIEQGKEN 322
FFICSAV+L+YY+ GLHATYDVAHQD S+ E+ K++
Sbjct: 551 FFICSAVMLLYYLFIGLHATYDVAHQDGLGSKNEEIKDD 589
>emb|CBI33021.3| unnamed protein product [Vitis vinifera]
Length = 595
Score = 140 bits (352), Expect = 2e-31
Identities = 63/94 (67%), Positives = 75/94 (79%), Gaps = 1/94 (1%)
Frame = +2
Query: 2 WN-SKYGRWWIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIG 178
WN +K G WIGY +A W + TLGM L+PKQR VWGVPLVPW+P+LS+ MN+FL+G
Sbjct: 455 WNPNKTG--WIGYAVAAFIWFLRTLGMALLPKQRVANVWGVPLVPWLPSLSVAMNLFLVG 512
Query: 179 SLGYVAFLRFFICSAVILVYYMLFGLHATYDVAH 280
SLGY AFLRF ICSAV+L+YY+ GLHATYDVAH
Sbjct: 513 SLGYQAFLRFVICSAVMLIYYLFIGLHATYDVAH 546
>gb|EXB52061.1| Cationic amino acid transporter 8 [Morus notabilis]
Length = 588
Score = 137 bits (344), Expect = 2e-30
Identities = 62/95 (65%), Positives = 74/95 (77%)
Frame = +2
Query: 2 WNSKYGRWWIGYVIAGIFWVIGTLGMELIPKQRAPKVWGVPLVPWIPALSIGMNIFLIGS 181
WN+ WIGY + + W +G LGM L+PKQR +VWGVPLVPW+P+LSIGMNI LIGS
Sbjct: 483 WNTNV-EGWIGYSVGAVIWFLGNLGMALLPKQRLTQVWGVPLVPWLPSLSIGMNILLIGS 541
Query: 182 LGYVAFLRFFICSAVILVYYMLFGLHATYDVAHQD 286
LG VAF RFFICSA++L YY+ GLHATYDVA+ D
Sbjct: 542 LGTVAFWRFFICSALMLGYYLFIGLHATYDVANPD 576