BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Paeonia22_contig00019629
         (2116 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_007029575.1| 4-coumarate:CoA ligase 3 [Theobroma cacao] g...   901   0.0  
gb|AEX32786.1| coumaroyl-CoA ligase [Vitis vinifera]                  892   0.0  
gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]                  891   0.0  
ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis ...   890   0.0  
ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus ...   887   0.0  
gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]        886   0.0  
gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum]          884   0.0  
gb|AHL44980.1| 4-coumarate:coenzyme A ligase 4 [Fraxinus mandshu...   883   0.0  
gb|ACZ06243.1| 4-coumarate:CoA ligase 2 [Gossypium hirsutum]          879   0.0  
gb|ADU15550.1| 4CL [Gossypium hirsutum]                               873   0.0  
ref|XP_006443197.1| hypothetical protein CICLE_v10019532mg [Citr...   870   0.0  
gb|AGW16013.1| 4-coumarate:coenzyme A ligase 1 [Pueraria montana...   869   0.0  
gb|AFG30056.1| 4-coumarate: CoA ligase [Malus hybrid cultivar]        869   0.0  
gb|AFC89538.1| 4-coumarate: coenzyme A ligase 2 [Populus tomentosa]   869   0.0  
gb|ADE96997.1| p-coumarate:CoA-ligase 3 [Sorbus aucuparia]            867   0.0  
ref|XP_004307864.1| PREDICTED: LOW QUALITY PROTEIN: 4-coumarate-...   864   0.0  
gb|AAC24504.1| 4-coumarate:CoA ligase [Populus tremuloides]           861   0.0  
ref|NP_001237270.1| 4-coumarate:CoA ligase isoenzyme 3 [Glycine ...   860   0.0  
gb|EYU45376.1| hypothetical protein MIMGU_mgv1a003734mg [Mimulus...   858   0.0  
ref|XP_002325815.1| 4-coumarate-CoA ligase family protein [Popul...   858   0.0  

>ref|XP_007029575.1| 4-coumarate:CoA ligase 3 [Theobroma cacao]
            gi|508718180|gb|EOY10077.1| 4-coumarate:CoA ligase 3
            [Theobroma cacao]
          Length = 575

 Score =  901 bits (2329), Expect = 0.0
 Identities = 452/567 (79%), Positives = 498/567 (87%), Gaps = 5/567 (0%)
 Frame = -1

Query: 1897 VETQKPDV---VLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTC 1727
            VE QKP++   +          T+ +H+F+SKLPDIPISNHLPLHTYCFE LS FPD+ C
Sbjct: 8    VEPQKPELSPSLSPPATKPSSETTGDHIFRSKLPDIPISNHLPLHTYCFENLSSFPDKPC 67

Query: 1726 LISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGA 1547
            LISGSS G+TYSF+ETHL  +KTAAGLSNLGI+KGDVIMILLQNC EFVFSF+GASMIGA
Sbjct: 68   LISGSS-GKTYSFSETHLIAQKTAAGLSNLGIRKGDVIMILLQNCAEFVFSFMGASMIGA 126

Query: 1546 ITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFG--ENGHKLGETYKIITVDDP 1373
            ++TTANPFYTS E+FKQ+  + AKLI+TQSQYVDKL D    EN  K+GE +K+IT+DDP
Sbjct: 127  VSTTANPFYTSTEIFKQYKVAQAKLIVTQSQYVDKLKDTSNNENFPKMGEDFKVITIDDP 186

Query: 1372 PKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVD 1193
            P+NCLHF+++S+ANE EIP V IDP+DPVALPFSSGTTGLPKGV+LTHKSLITSVAQQVD
Sbjct: 187  PENCLHFTVLSEANENEIPQVSIDPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVD 246

Query: 1192 GENPNLYLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQRYKV 1013
            GENPNLYL  DDVVLCVLPLFHIYSLNSVLLC+LRAGA VLLMQKFEIG LLE IQ++KV
Sbjct: 247  GENPNLYLKHDDVVLCVLPLFHIYSLNSVLLCALRAGAAVLLMQKFEIGALLELIQKHKV 306

Query: 1012 SXXXXXXXXXXXXAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGM 833
            S            AKNPMVA++DLSSIRVVLSGAAPLGKELE+ALR RVPQA+LGQGYGM
Sbjct: 307  SVAAVVPPLVLALAKNPMVAQYDLSSIRVVLSGAAPLGKELEEALRGRVPQAVLGQGYGM 366

Query: 832  TEAGPVLSMCLGFAKQSFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGPQI 653
            TEAGPVLSMCLGFAKQ FPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRG QI
Sbjct: 367  TEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGSQI 426

Query: 652  MKGYLNDVEATTTTIDIEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEAL 473
            MKGYLND  AT TTID+EGWLHTGDIGYVDD +E+FIVDRVKE+IKFKGFQVPPAELE+L
Sbjct: 427  MKGYLNDTSATATTIDVEGWLHTGDIGYVDDGDEIFIVDRVKEIIKFKGFQVPPAELESL 486

Query: 472  LVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHKVYF 293
            LVSHPSIADAAVVPQKD  AGEVPVAFVVRSNG ELTEE VK+FIAKQVVFYKKLHKV+F
Sbjct: 487  LVSHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAVKEFIAKQVVFYKKLHKVHF 546

Query: 292  IHAIPKSPSGKILRKDLRAKLGTTSPT 212
            +HAIPKSPSGKILRKDLRAKL   + T
Sbjct: 547  VHAIPKSPSGKILRKDLRAKLAAATTT 573


>gb|AEX32786.1| coumaroyl-CoA ligase [Vitis vinifera]
          Length = 570

 Score =  892 bits (2305), Expect = 0.0
 Identities = 445/570 (78%), Positives = 496/570 (87%), Gaps = 4/570 (0%)
 Frame = -1

Query: 1906 MISVETQKPDVVLXXXXXXXXXTSS---NHVFKSKLPDIPISNHLPLHTYCFEKLSEFPD 1736
            MIS+ETQ PDV            ++   +HVF+SKLP+IPISNHLPLHTYCFE  S+F D
Sbjct: 1    MISIETQNPDVSNLDTSHSIPKMANRIDDHVFRSKLPEIPISNHLPLHTYCFENYSQFAD 60

Query: 1735 RTCLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASM 1556
            R CLI GS+N +TYSFAETHL  +K  AG ++LG+K+GDV+MILLQNC EF FSF+GASM
Sbjct: 61   RPCLIVGSTN-KTYSFAETHLISRKVGAGFAHLGLKQGDVVMILLQNCAEFAFSFLGASM 119

Query: 1555 IGAITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFGENG-HKLGETYKIITVD 1379
            +GA+TTTANPFYTSAE+FKQ   S AK+++TQ+QYVDKL D+ +    K+GE + +IT+D
Sbjct: 120  VGAVTTTANPFYTSAEIFKQLNASKAKIVVTQAQYVDKLRDYPDGQVAKIGEGFTVITID 179

Query: 1378 DPPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQ 1199
            DPP+NC+HFS++S+ANE+E+P V I+ +DPVALPFSSGTTGLPKGV+LTHKSLITSVAQQ
Sbjct: 180  DPPENCMHFSVVSEANESELPEVSINSDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQ 239

Query: 1198 VDGENPNLYLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQRY 1019
            VDGENPNL+LTPDDVVLCVLPLFHIYSLNSVLLCSLRAGA VLLMQKFEIGTLLE IQRY
Sbjct: 240  VDGENPNLHLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELIQRY 299

Query: 1018 KVSXXXXXXXXXXXXAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGY 839
            +VS            AKNPMV  FDLSSIRVVLSGAAPLGKELE ALRSRVPQA+LGQGY
Sbjct: 300  RVSVAAVVPPLVLALAKNPMVESFDLSSIRVVLSGAAPLGKELEAALRSRVPQAVLGQGY 359

Query: 838  GMTEAGPVLSMCLGFAKQSFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGP 659
            GMTEAGPVLSMCLGFAKQ FPTKSGSCGTVVRNAELKV+DPETGCSLG NQPGEICIRG 
Sbjct: 360  GMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVVDPETGCSLGRNQPGEICIRGQ 419

Query: 658  QIMKGYLNDVEATTTTIDIEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELE 479
            QIMKGYLND EAT +TID++GWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELE
Sbjct: 420  QIMKGYLNDPEATASTIDVDGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELE 479

Query: 478  ALLVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHKV 299
            ALLVSHPSIADAAVVPQKD  AGEVPVAFVVRSNG ELTEE VK+FI+KQVVFYK+LHKV
Sbjct: 480  ALLVSHPSIADAAVVPQKDDVAGEVPVAFVVRSNGFELTEEAVKEFISKQVVFYKRLHKV 539

Query: 298  YFIHAIPKSPSGKILRKDLRAKLGTTSPTP 209
            YF+HAIPKSPSGKILRKDLRAKL   +P P
Sbjct: 540  YFVHAIPKSPSGKILRKDLRAKLAEKTPEP 569


>gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]
          Length = 604

 Score =  891 bits (2302), Expect = 0.0
 Identities = 447/551 (81%), Positives = 488/551 (88%), Gaps = 8/551 (1%)
 Frame = -1

Query: 1837 SSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCLISGSSNGRTYSFAETHLACKKT 1658
            ++NHVFKSKLPDIPI NHLPLHTYCF+KLSEF DR CLI  SS G++YSF++T+L  +KT
Sbjct: 55   TTNHVFKSKLPDIPIPNHLPLHTYCFQKLSEFSDRPCLIV-SSTGKSYSFSDTYLISQKT 113

Query: 1657 AAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAITTTANPFYTSAEVFKQFTTSNA 1478
             AGLSNLGIKKGDVIMILLQNC EFVF+F+GASMIGAITTTANPFYT+ E+FKQF  SNA
Sbjct: 114  GAGLSNLGIKKGDVIMILLQNCAEFVFAFMGASMIGAITTTANPFYTATEIFKQFKNSNA 173

Query: 1477 KLIITQSQYVDKLVDFGENGH--------KLGETYKIITVDDPPKNCLHFSIISDANETE 1322
            KLIIT SQ+VDKL +   +G         KLGE +K++TVDDPP+NCLHFS+IS+ANE E
Sbjct: 174  KLIITHSQFVDKLREAPADGAAPDQNNYLKLGEHFKVVTVDDPPENCLHFSVISEANEKE 233

Query: 1321 IPSVFIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLTPDDVVLCV 1142
            +P V IDP DPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYL  DDVVLCV
Sbjct: 234  LPEVVIDPEDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCV 293

Query: 1141 LPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQRYKVSXXXXXXXXXXXXAKNP 962
            LPLFHIYSLNSV+LCSLRAGA VL+M KFEIGTLLE IQRY+VS            AKNP
Sbjct: 294  LPLFHIYSLNSVMLCSLRAGAAVLVMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKNP 353

Query: 961  MVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQS 782
            MVA+FDLSSIRVVLSGAAPLGKELE+ALRSRVP A+LGQGYGMTEAGPVLSMCL FAK+ 
Sbjct: 354  MVAQFDLSSIRVVLSGAAPLGKELEEALRSRVPGAVLGQGYGMTEAGPVLSMCLAFAKEP 413

Query: 781  FPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGPQIMKGYLNDVEATTTTIDI 602
             P+KSGSCGTV+RNAELKVID ETGCSLGYNQPGEICIRG QIMKGYLND EAT TT+D 
Sbjct: 414  LPSKSGSCGTVIRNAELKVIDSETGCSLGYNQPGEICIRGSQIMKGYLNDAEATATTVDK 473

Query: 601  EGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKD 422
            EGWLHTGD+GYVDDDEEVFIVDRVKELIKFKGFQVPPAELE+LLVSHPSIADAAVVPQKD
Sbjct: 474  EGWLHTGDVGYVDDDEEVFIVDRVKELIKFKGFQVPPAELESLLVSHPSIADAAVVPQKD 533

Query: 421  VAAGEVPVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHKVYFIHAIPKSPSGKILRKDL 242
             AAGEVPVAFVVRSNG++LTEE VK+FIAKQVVFYK+LHKVYF+HAIPKSPSGKILRKDL
Sbjct: 534  EAAGEVPVAFVVRSNGLQLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDL 593

Query: 241  RAKLGTTSPTP 209
            RAKL T +P P
Sbjct: 594  RAKLATPTPLP 604


>ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis vinifera]
          Length = 570

 Score =  890 bits (2299), Expect = 0.0
 Identities = 444/570 (77%), Positives = 495/570 (86%), Gaps = 4/570 (0%)
 Frame = -1

Query: 1906 MISVETQKPDVVLXXXXXXXXXTSS---NHVFKSKLPDIPISNHLPLHTYCFEKLSEFPD 1736
            MIS+ETQ PDV            ++   +HVF+SKLP+IPISNHLPLHTYCFE  S+F D
Sbjct: 1    MISIETQNPDVSNLDTSHSIPKMANRIDDHVFRSKLPEIPISNHLPLHTYCFENYSQFAD 60

Query: 1735 RTCLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASM 1556
            R CLI GS+N +TYSFAETHL  +K  AG ++LG+K+GDV+MILLQNC EF FSF+GASM
Sbjct: 61   RPCLIVGSTN-KTYSFAETHLISRKVGAGFAHLGLKQGDVVMILLQNCAEFAFSFLGASM 119

Query: 1555 IGAITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFGENG-HKLGETYKIITVD 1379
            +GA+TTTANPFYTSAE+FKQ   S AK+++TQ+QYVDKL D+ +    K+GE + +IT+D
Sbjct: 120  VGAVTTTANPFYTSAEIFKQLNASKAKIVVTQAQYVDKLRDYPDGQVAKIGEGFTVITID 179

Query: 1378 DPPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQ 1199
            DPP+NC+HFS++S+ANE+E+P V I+ +DPVALPFSSGTTGLPKGV+LTHKSLITSVAQQ
Sbjct: 180  DPPENCMHFSVVSEANESELPEVSINSDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQ 239

Query: 1198 VDGENPNLYLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQRY 1019
            VDGENPNL+LTPDDVVLCVLPLFHIYSLNSVLLCSLRAGA VLLMQKFEIGTLLE IQRY
Sbjct: 240  VDGENPNLHLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELIQRY 299

Query: 1018 KVSXXXXXXXXXXXXAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGY 839
            +VS            AKNPMV  FDLSSIRVVLSGAAPLGKELE ALRSRVPQA+LGQGY
Sbjct: 300  RVSVAAVVPPLVLALAKNPMVESFDLSSIRVVLSGAAPLGKELEAALRSRVPQAVLGQGY 359

Query: 838  GMTEAGPVLSMCLGFAKQSFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGP 659
            GMTEAGPVLSMCL FAKQ FPTKSGSCGTVVRNAELKV+DPETGCSLG NQPGEICIRG 
Sbjct: 360  GMTEAGPVLSMCLAFAKQPFPTKSGSCGTVVRNAELKVVDPETGCSLGRNQPGEICIRGQ 419

Query: 658  QIMKGYLNDVEATTTTIDIEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELE 479
            QIMKGYLND EAT +TID++GWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELE
Sbjct: 420  QIMKGYLNDPEATASTIDVDGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELE 479

Query: 478  ALLVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHKV 299
            ALLVSHPSIADAAVVPQKD  AGEVPVAFVVRSNG ELTEE VK+FI+KQVVFYK+LHKV
Sbjct: 480  ALLVSHPSIADAAVVPQKDDVAGEVPVAFVVRSNGFELTEEAVKEFISKQVVFYKRLHKV 539

Query: 298  YFIHAIPKSPSGKILRKDLRAKLGTTSPTP 209
            YF+HAIPKSPSGKILRKDLRAKL   +P P
Sbjct: 540  YFVHAIPKSPSGKILRKDLRAKLAEKTPEP 569


>ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus communis]
            gi|223540792|gb|EEF42352.1| AMP dependent CoA ligase,
            putative [Ricinus communis]
          Length = 573

 Score =  887 bits (2293), Expect = 0.0
 Identities = 448/543 (82%), Positives = 485/543 (89%), Gaps = 2/543 (0%)
 Frame = -1

Query: 1837 SSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCLISGSSNGRTYSFAETHLACKKT 1658
            S  H+F+SKLPDIPISNHLPLHTYCFE L +  D+ CLISGS+ G+TYSFAETHL  +KT
Sbjct: 30   SETHIFRSKLPDIPISNHLPLHTYCFENLFKLSDKPCLISGST-GKTYSFAETHLISQKT 88

Query: 1657 AAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAITTTANPFYTSAEVFKQFTTSNA 1478
            AAGLS+LGIKKGDVIMILLQNCPEFVFSF+GASMIGA+ TTANPFYTS E+FKQ  +S  
Sbjct: 89   AAGLSSLGIKKGDVIMILLQNCPEFVFSFMGASMIGAVATTANPFYTSNEIFKQLNSSRP 148

Query: 1477 KLIITQSQYVDKLVDFGENGH-KLGETYKIITVDDPPKNCLHFSIISDANETEIPSVF-I 1304
            KLIITQS YVDKL D       KLGE + +IT+D+PP+NCLHF+++S+A+ETEIP V   
Sbjct: 149  KLIITQSLYVDKLRDPDHKIQPKLGEDFTVITIDEPPENCLHFTVLSEASETEIPEVINA 208

Query: 1303 DPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLTPDDVVLCVLPLFHI 1124
            +P+DPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYL  DDVVLCVLPLFHI
Sbjct: 209  NPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPLFHI 268

Query: 1123 YSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQRYKVSXXXXXXXXXXXXAKNPMVAEFD 944
            YSLNSVLLCSLRAGA VLLMQKF+IG LLE IQ++KVS            AKNPMVAEFD
Sbjct: 269  YSLNSVLLCSLRAGAAVLLMQKFQIGALLELIQKHKVSVAAVVPPLVLALAKNPMVAEFD 328

Query: 943  LSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQSFPTKSG 764
            LSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQ FPTKSG
Sbjct: 329  LSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQPFPTKSG 388

Query: 763  SCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGPQIMKGYLNDVEATTTTIDIEGWLHT 584
            SCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGPQIMKGYLND EAT  TID+EGWLHT
Sbjct: 389  SCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGPQIMKGYLNDPEATANTIDVEGWLHT 448

Query: 583  GDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDVAAGEV 404
            GDIGYVDDD+E+FIVDRVKE+IKFKGFQVPPAELEALL++HPSIADAAVVPQKD  AGEV
Sbjct: 449  GDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAELEALLLNHPSIADAAVVPQKDEVAGEV 508

Query: 403  PVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHKVYFIHAIPKSPSGKILRKDLRAKLGT 224
            PVAFVVRSNG EL+EE VK++IAKQVVFYKKLHKVYFIHAIPKSPSGKILRKDL+AKL T
Sbjct: 509  PVAFVVRSNGFELSEEDVKEYIAKQVVFYKKLHKVYFIHAIPKSPSGKILRKDLKAKLAT 568

Query: 223  TSP 215
             SP
Sbjct: 569  PSP 571


>gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]
          Length = 565

 Score =  886 bits (2289), Expect = 0.0
 Identities = 443/540 (82%), Positives = 485/540 (89%)
 Frame = -1

Query: 1831 NHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCLISGSSNGRTYSFAETHLACKKTAA 1652
            N +F+SKLPDIPISNHLPLHTYCFEK++EF D  CLISGSS GR Y+FAETHL C+K+AA
Sbjct: 27   NFIFRSKLPDIPISNHLPLHTYCFEKITEFSDAPCLISGSS-GRIYTFAETHLICQKSAA 85

Query: 1651 GLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAITTTANPFYTSAEVFKQFTTSNAKL 1472
            GLS LGIKKGDVIMILLQN PEF FSF+GASMIGAITTTANPF TSAE+FKQF+ S AKL
Sbjct: 86   GLSKLGIKKGDVIMILLQNSPEFAFSFMGASMIGAITTTANPFCTSAEIFKQFSVSGAKL 145

Query: 1471 IITQSQYVDKLVDFGENGHKLGETYKIITVDDPPKNCLHFSIISDANETEIPSVFIDPND 1292
            +ITQSQYVDKL D GE   K+GE + +ITVDDPP++CLHFS+IS A+E EIPSV IDP+D
Sbjct: 146  VITQSQYVDKLRDTGEKFPKIGEDFTVITVDDPPESCLHFSVISGADEREIPSVSIDPDD 205

Query: 1291 PVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLTPDDVVLCVLPLFHIYSLN 1112
            PV++PFSSGTTGLPKGV LTHKSLI+SVAQQVDGENPNLYL  DD+VLCVLPLFHIYSLN
Sbjct: 206  PVSMPFSSGTTGLPKGVFLTHKSLISSVAQQVDGENPNLYLKTDDIVLCVLPLFHIYSLN 265

Query: 1111 SVLLCSLRAGAGVLLMQKFEIGTLLEFIQRYKVSXXXXXXXXXXXXAKNPMVAEFDLSSI 932
            SVLLCSLRAGAGVLLM KFEI +LLE IQR++VS            AKNPMVA FDLSSI
Sbjct: 266  SVLLCSLRAGAGVLLMHKFEIRSLLELIQRHRVSVAPVVPPLVLALAKNPMVASFDLSSI 325

Query: 931  RVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQSFPTKSGSCGT 752
            R+VLSGAAPLGKELE+AL SR+PQAI GQGYGMTEAGPVLSMCLGFA+Q+FPTKSGSCGT
Sbjct: 326  RIVLSGAAPLGKELEEALHSRLPQAIFGQGYGMTEAGPVLSMCLGFARQAFPTKSGSCGT 385

Query: 751  VVRNAELKVIDPETGCSLGYNQPGEICIRGPQIMKGYLNDVEATTTTIDIEGWLHTGDIG 572
            VVRNAELKVIDPETG SL YNQPGEICIRGPQIMKGYLND EAT +TID++GWLHTGD+G
Sbjct: 386  VVRNAELKVIDPETGFSLQYNQPGEICIRGPQIMKGYLNDAEATASTIDVDGWLHTGDVG 445

Query: 571  YVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDVAAGEVPVAF 392
            YVDDD+EVFIVDRVKELIKFKGFQVPPAELEALL+SHPSIADAAVVPQKD AAGEVPVAF
Sbjct: 446  YVDDDDEVFIVDRVKELIKFKGFQVPPAELEALLISHPSIADAAVVPQKDEAAGEVPVAF 505

Query: 391  VVRSNGIELTEETVKDFIAKQVVFYKKLHKVYFIHAIPKSPSGKILRKDLRAKLGTTSPT 212
            VVRS+G ELTEE VK+FIAKQVVFYK+LHKVYF+HAIPKS SGKIL KDLRAKL T SP+
Sbjct: 506  VVRSSGFELTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSASGKILGKDLRAKLATGSPS 565


>gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum]
          Length = 573

 Score =  884 bits (2285), Expect = 0.0
 Identities = 446/568 (78%), Positives = 492/568 (86%), Gaps = 5/568 (0%)
 Frame = -1

Query: 1903 ISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCL 1724
            + +E+ KPD +          T ++H+F+SKLPDIPISNHLPLHTYCFE L    D+ CL
Sbjct: 9    VELESPKPDQL-------SPETPADHIFRSKLPDIPISNHLPLHTYCFENLPSLSDKPCL 61

Query: 1723 ISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAI 1544
            ISGSS G+ YSF+ETHL  +KTAAGLSNLGI+KGD IMILL NC EFVF+F+GASMIGA+
Sbjct: 62   ISGSS-GKIYSFSETHLIAQKTAAGLSNLGIQKGDAIMILLHNCAEFVFAFMGASMIGAV 120

Query: 1543 TTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFG-----ENGHKLGETYKIITVD 1379
            +TTANPFYTS E+FKQF  + AKLIITQSQYVDKL D       EN  K+GE +K+IT+D
Sbjct: 121  STTANPFYTSKEIFKQFKAARAKLIITQSQYVDKLKDTNNNDGDENFPKIGEDFKVITID 180

Query: 1378 DPPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQ 1199
            DPP+NCL FS++S+ANE EIP V I P+DPVALPFSSGTTGLPKGV+LTHKSLITSVAQQ
Sbjct: 181  DPPENCLDFSMLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQ 240

Query: 1198 VDGENPNLYLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQRY 1019
            VDGENPNLYLT DDVVLCVLPLFHIYSLNSVLLCSLRAGA VLLMQKFEIGTLLE IQR+
Sbjct: 241  VDGENPNLYLTHDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELIQRH 300

Query: 1018 KVSXXXXXXXXXXXXAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGY 839
            KVS            AKNP+VA+FDLSSIRVVLSGAAPLGKELEDALR RVPQA+LGQGY
Sbjct: 301  KVSVAAVVPPLVVLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEDALRGRVPQAVLGQGY 360

Query: 838  GMTEAGPVLSMCLGFAKQSFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGP 659
            GMTEAGPVLSMCLGFAKQ FPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRG 
Sbjct: 361  GMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGS 420

Query: 658  QIMKGYLNDVEATTTTIDIEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELE 479
            QIMKGYLND  AT  TID+EGWLHTGDIGYVD+D+E+FIVDRVKE+IKFKGFQVPPAELE
Sbjct: 421  QIMKGYLNDGVATAATIDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQVPPAELE 480

Query: 478  ALLVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHKV 299
            +LL+SHPSIADAAVVPQKD  AGEVPVAFVVRSNG ELTEE VK+FIAKQVV+YK+LHKV
Sbjct: 481  SLLISHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAVKEFIAKQVVYYKRLHKV 540

Query: 298  YFIHAIPKSPSGKILRKDLRAKLGTTSP 215
            +F+HAIPKSPSGKILRKDLRAKL   +P
Sbjct: 541  HFVHAIPKSPSGKILRKDLRAKLAAAAP 568


>gb|AHL44980.1| 4-coumarate:coenzyme A ligase 4 [Fraxinus mandshurica]
          Length = 573

 Score =  883 bits (2282), Expect = 0.0
 Identities = 447/569 (78%), Positives = 494/569 (86%), Gaps = 2/569 (0%)
 Frame = -1

Query: 1909 TMISVETQKPDVVLXXXXXXXXXTSSN--HVFKSKLPDIPISNHLPLHTYCFEKLSEFPD 1736
            ++ SVETQ  ++            SS   HVF SKLP IPISNHLPLHTYCF  L+++P+
Sbjct: 3    SVASVETQNSELSSLASPQKPQTQSSEETHVFISKLPQIPISNHLPLHTYCFGNLAQYPE 62

Query: 1735 RTCLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASM 1556
            R CLI GS+ G+TYSF+ETHL C+KTAAGLS LG+KKGDVIM+LLQNCPEFVF+F+GASM
Sbjct: 63   RPCLIVGST-GKTYSFSETHLLCRKTAAGLSKLGLKKGDVIMVLLQNCPEFVFTFMGASM 121

Query: 1555 IGAITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFGENGHKLGETYKIITVDD 1376
            IGA+TTTANPFYTS+EVFKQF  S AK+IITQSQYVDKL D G+N  KLGE + +IT+DD
Sbjct: 122  IGAVTTTANPFYTSSEVFKQFGASEAKMIITQSQYVDKLRDPGQNNPKLGEDFTVITIDD 181

Query: 1375 PPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQV 1196
            PP+NCLHFS++S+ANE +IPSV IDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQV
Sbjct: 182  PPENCLHFSVLSEANENDIPSVSIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQV 241

Query: 1195 DGENPNLYLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQRYK 1016
            DGENPNLYL  DDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIG+ LE IQR++
Sbjct: 242  DGENPNLYLKSDDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGSFLELIQRHR 301

Query: 1015 VSXXXXXXXXXXXXAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYG 836
            VS            AKNP+V +FDLSSIRVVLSGAAPLGKELE ALR+RVPQAI GQGYG
Sbjct: 302  VSVAAVVPPLVLALAKNPLVDKFDLSSIRVVLSGAAPLGKELEAALRNRVPQAIFGQGYG 361

Query: 835  MTEAGPVLSMCLGFAKQSFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGPQ 656
            MTEAGPVLSM   FAKQ   TKSGSCG VVRNAELKV+DPE+G SL  NQPGEICIRGPQ
Sbjct: 362  MTEAGPVLSMSTAFAKQPILTKSGSCGNVVRNAELKVVDPESGSSLPRNQPGEICIRGPQ 421

Query: 655  IMKGYLNDVEATTTTIDIEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEA 476
            IMKGYLND EAT  TID++GWLHTGDIGYVDDD+++FIVDRVKELIKFKGFQVPPAELEA
Sbjct: 422  IMKGYLNDDEATANTIDVDGWLHTGDIGYVDDDDDIFIVDRVKELIKFKGFQVPPAELEA 481

Query: 475  LLVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHKVY 296
            LLVSHP+IADAAVVPQKD AAGEVPVAFVVRSNG EL+EE VK+FIAKQVVFYKKLHKVY
Sbjct: 482  LLVSHPNIADAAVVPQKDEAAGEVPVAFVVRSNGFELSEEAVKEFIAKQVVFYKKLHKVY 541

Query: 295  FIHAIPKSPSGKILRKDLRAKLGTTSPTP 209
            FIHAIPKSP+GKILRKDLRAKL TT+ +P
Sbjct: 542  FIHAIPKSPAGKILRKDLRAKLVTTTSSP 570


>gb|ACZ06243.1| 4-coumarate:CoA ligase 2 [Gossypium hirsutum]
          Length = 574

 Score =  879 bits (2270), Expect = 0.0
 Identities = 442/570 (77%), Positives = 491/570 (86%), Gaps = 6/570 (1%)
 Frame = -1

Query: 1903 ISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCL 1724
            + +E+ KPD +          T ++H+F+SKLPDIPISNHLPLHTYCFE L    D+ CL
Sbjct: 9    VELESPKPDQL-------SPETPADHIFRSKLPDIPISNHLPLHTYCFENLPSLSDKPCL 61

Query: 1723 ISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAI 1544
            ISGSS G+ YSF+ETHL  +KTAAGLS LGI+KGD IMILL NC EFVF+F+GASMIGA+
Sbjct: 62   ISGSS-GKIYSFSETHLIAQKTAAGLSKLGIQKGDAIMILLHNCAEFVFAFMGASMIGAV 120

Query: 1543 TTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFG------ENGHKLGETYKIITV 1382
            +TTANPFYTS E+FKQF  + AKLIITQSQYVDKL D        EN  K+GE +K+IT+
Sbjct: 121  STTANPFYTSKEIFKQFKAARAKLIITQSQYVDKLKDTNNNNDGVENFPKIGEDFKVITI 180

Query: 1381 DDPPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQ 1202
            DDPP+NCL FS++S+ANE EIP V I P+DPVALPFSSGTTGLPKGV+LTHKSLITSVAQ
Sbjct: 181  DDPPENCLDFSVLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQ 240

Query: 1201 QVDGENPNLYLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQR 1022
            QVDGENPNLYLT +DVVLCVLPLFHIYSLNSVLLCSLRAGA VLLMQKFEIGTLLE IQR
Sbjct: 241  QVDGENPNLYLTHEDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELIQR 300

Query: 1021 YKVSXXXXXXXXXXXXAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQG 842
            +KVS            AKNP+VA+FDLSSIRVVLSGAAPLGKELE+ALR RVPQA+LGQG
Sbjct: 301  HKVSVAAVVPPLVLLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEEALRGRVPQAVLGQG 360

Query: 841  YGMTEAGPVLSMCLGFAKQSFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRG 662
            YGMTEAGPVLSMCLGFAKQ FPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRG
Sbjct: 361  YGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRG 420

Query: 661  PQIMKGYLNDVEATTTTIDIEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAEL 482
             QIMKGYLND  AT  T D+EGWLHTGDIGYVD+D+E+FIVDRVKE+IKFKGFQVPPAEL
Sbjct: 421  SQIMKGYLNDSVATAATTDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQVPPAEL 480

Query: 481  EALLVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHK 302
            E+LL+SHPSIADAAVVPQKD  AGEVPVAFVVRSNG ELTEE VK+FIA+QVV+YK+LHK
Sbjct: 481  ESLLISHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAVKEFIARQVVYYKRLHK 540

Query: 301  VYFIHAIPKSPSGKILRKDLRAKLGTTSPT 212
            V+F+HAIPKSPSGKILRKDLRAKL   +PT
Sbjct: 541  VHFVHAIPKSPSGKILRKDLRAKLAAAAPT 570


>gb|ADU15550.1| 4CL [Gossypium hirsutum]
          Length = 574

 Score =  873 bits (2255), Expect = 0.0
 Identities = 442/569 (77%), Positives = 488/569 (85%), Gaps = 6/569 (1%)
 Frame = -1

Query: 1903 ISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCL 1724
            + +E  KPD +          T ++H+F+SKLPDIPISNHLPLHTYCFE L    D+ CL
Sbjct: 9    VELEPPKPDQL-------SPETPADHIFRSKLPDIPISNHLPLHTYCFENLPSLSDKPCL 61

Query: 1723 ISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAI 1544
            ISGSS G+ YSF+ETHL  +KTAAGLS LGI+KGD IMILL NC EFVF+F+GASMIGA+
Sbjct: 62   ISGSS-GKIYSFSETHLIAQKTAAGLSKLGIQKGDAIMILLHNCAEFVFAFMGASMIGAV 120

Query: 1543 TTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFG------ENGHKLGETYKIITV 1382
            +TTANPFYTS E+FKQ   + AKLIITQSQYVDKL D        EN  K+GE +K+IT+
Sbjct: 121  STTANPFYTSKEIFKQSKAARAKLIITQSQYVDKLKDTNNNNDGVENFPKIGEDFKVITI 180

Query: 1381 DDPPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQ 1202
            DDPP+NCL FS++S+ANE EIP V I P+DPVALPFSSGTTGLPKGV+LTHKSLITSVAQ
Sbjct: 181  DDPPENCLDFSVLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQ 240

Query: 1201 QVDGENPNLYLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQR 1022
            QVDGENPNLYLT +DVVLCVLPLFHIYSLNSVLL SLRAGA VLLMQKFEIGTLLE IQR
Sbjct: 241  QVDGENPNLYLTHEDVVLCVLPLFHIYSLNSVLLWSLRAGAAVLLMQKFEIGTLLELIQR 300

Query: 1021 YKVSXXXXXXXXXXXXAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQG 842
            +KVS            AKNP+VA+FDLSSIRVVLSGAAPLGKELEDALR RVPQA+LGQG
Sbjct: 301  HKVSVAAVVPPLVVLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEDALRGRVPQAVLGQG 360

Query: 841  YGMTEAGPVLSMCLGFAKQSFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRG 662
            YGMTEAGPVLSMCLGFAKQ FPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRG
Sbjct: 361  YGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRG 420

Query: 661  PQIMKGYLNDVEATTTTIDIEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAEL 482
             QIMKGYLND  AT  TID+EGWLHTGDIGYVD+D+E+FIVDRVKE+IKFKGFQVPPAEL
Sbjct: 421  SQIMKGYLNDGVATAATIDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQVPPAEL 480

Query: 481  EALLVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHK 302
            E+LL+SHPSIADAAVVPQKD  AGEVPVAFVVRSNG ELTEE VK+FIAKQVV+YK+LHK
Sbjct: 481  ESLLISHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAVKEFIAKQVVYYKRLHK 540

Query: 301  VYFIHAIPKSPSGKILRKDLRAKLGTTSP 215
            V+F+HAIPKSPSGKILRKDLRAKL   +P
Sbjct: 541  VHFVHAIPKSPSGKILRKDLRAKLAAAAP 569


>ref|XP_006443197.1| hypothetical protein CICLE_v10019532mg [Citrus clementina]
            gi|568850424|ref|XP_006478914.1| PREDICTED:
            4-coumarate--CoA ligase 2-like [Citrus sinensis]
            gi|557545459|gb|ESR56437.1| hypothetical protein
            CICLE_v10019532mg [Citrus clementina]
          Length = 560

 Score =  870 bits (2249), Expect = 0.0
 Identities = 443/569 (77%), Positives = 492/569 (86%), Gaps = 5/569 (0%)
 Frame = -1

Query: 1906 MISVETQKPDVVLXXXXXXXXXTSSN----HVFKSKLPDIPISNHLPLHTYCFE-KLSEF 1742
            MIS+ T+KP++ L          S+     H+FKSKLPDIPISNHLPLHTYCF+ +LS+ 
Sbjct: 1    MISIATKKPELSLDISSPAPPAPSNEKIATHIFKSKLPDIPISNHLPLHTYCFQDRLSDD 60

Query: 1741 PDRTCLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGA 1562
            P   CLI G + G+TYS+AETHL C+KTAAGLSNLGIKKGDVIMILLQNC EFVFSF+GA
Sbjct: 61   P---CLIVGLT-GKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGA 116

Query: 1561 SMIGAITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFGENGHKLGETYKIITV 1382
            SMIGA+TTTANPFYTSAE+ KQF TS AKLIIT SQYVD+L        K  + + +IT+
Sbjct: 117  SMIGAVTTTANPFYTSAEILKQFRTSGAKLIITMSQYVDRLP-------KTDKDFTVITI 169

Query: 1381 DDPPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQ 1202
            D PP+NCLHF+++S+A+E +IP V I+P+DPVALPFSSGTTGLPKGV+LTHKSLITSVAQ
Sbjct: 170  DAPPENCLHFTVLSEADEDQIPEVAIEPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQ 229

Query: 1201 QVDGENPNLYLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQR 1022
            QVDGENPNLYLT  DVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIG LLE IQR
Sbjct: 230  QVDGENPNLYLTNGDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGALLELIQR 289

Query: 1021 YKVSXXXXXXXXXXXXAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQG 842
            ++VS            AKNPMVA++DLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQG
Sbjct: 290  HRVSVAAVVPPLVLALAKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQG 349

Query: 841  YGMTEAGPVLSMCLGFAKQSFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRG 662
            YGMTEAGPVLSMCLGFAKQ FPTKSGSCGTVVRNAELKVIDPE G SL +NQPGEICIRG
Sbjct: 350  YGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPEIGASLPHNQPGEICIRG 409

Query: 661  PQIMKGYLNDVEATTTTIDIEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAEL 482
            PQIMKGYLND EAT  TID+EGWLHTGDIGYVDDD+EVFIVDRVKE+IKFKGFQVPPAE+
Sbjct: 410  PQIMKGYLNDPEATAATIDVEGWLHTGDIGYVDDDDEVFIVDRVKEIIKFKGFQVPPAEI 469

Query: 481  EALLVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHK 302
            EALL+SHPSI DAAVVPQKD  AGEVPVAFVVRSNG ELTEE +K++IAKQVVFYK+LHK
Sbjct: 470  EALLLSHPSIGDAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAIKEYIAKQVVFYKRLHK 529

Query: 301  VYFIHAIPKSPSGKILRKDLRAKLGTTSP 215
            +YF+HAIPKSPSGKILRKDLRAKL ++ P
Sbjct: 530  IYFVHAIPKSPSGKILRKDLRAKLASSMP 558


>gb|AGW16013.1| 4-coumarate:coenzyme A ligase 1 [Pueraria montana var. lobata]
          Length = 567

 Score =  869 bits (2246), Expect = 0.0
 Identities = 431/535 (80%), Positives = 482/535 (90%)
 Frame = -1

Query: 1834 SNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCLISGSSNGRTYSFAETHLACKKTA 1655
            + HVFKSKLPDIPISNH+PLH YCFEKLS+F DR CLI G+++ +TY++AETHL  +K A
Sbjct: 28   TTHVFKSKLPDIPISNHIPLHAYCFEKLSDFADRPCLIVGAAS-KTYTYAETHLISRKIA 86

Query: 1654 AGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAITTTANPFYTSAEVFKQFTTSNAK 1475
            AGLSNLGI+KGDV+MILLQN  EFVFSF+ ASMIGA+ TTANPFYT+AE+FKQFT S AK
Sbjct: 87   AGLSNLGIRKGDVVMILLQNSAEFVFSFLAASMIGAVGTTANPFYTAAEIFKQFTVSKAK 146

Query: 1474 LIITQSQYVDKLVDFGENGHKLGETYKIITVDDPPKNCLHFSIISDANETEIPSVFIDPN 1295
            LIITQ+ YVDKL     +G  LGE +K++TVDDPP+NCLHFS++S+ANE+E+P V I P+
Sbjct: 147  LIITQAMYVDKLRH--HDGTNLGEDFKVVTVDDPPENCLHFSVLSEANESEVPEVEIHPD 204

Query: 1294 DPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLTPDDVVLCVLPLFHIYSL 1115
            D VA+PFSSGTTGLPKGV+LTHKSL TSVAQQVDGENPNLYLT +DV+LCVLPLFHI+SL
Sbjct: 205  DAVAMPFSSGTTGLPKGVVLTHKSLTTSVAQQVDGENPNLYLTTEDVLLCVLPLFHIFSL 264

Query: 1114 NSVLLCSLRAGAGVLLMQKFEIGTLLEFIQRYKVSXXXXXXXXXXXXAKNPMVAEFDLSS 935
            NSVLLC+LRAG+GVLLMQKFEIGTLLE IQR++VS            AKNPMVA+FDLSS
Sbjct: 265  NSVLLCALRAGSGVLLMQKFEIGTLLELIQRHRVSVAMVVPPLVLALAKNPMVADFDLSS 324

Query: 934  IRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQSFPTKSGSCG 755
            IR+VLSGAAPLGKELE+ALR+RVPQA+LGQGYGMTEAGPVLSMCLGFAKQ FPTKSGSCG
Sbjct: 325  IRLVLSGAAPLGKELEEALRNRVPQAVLGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCG 384

Query: 754  TVVRNAELKVIDPETGCSLGYNQPGEICIRGPQIMKGYLNDVEATTTTIDIEGWLHTGDI 575
            TVVRNAELKV+DPETG SLGYNQPGEICIRG QIMKGYLND +AT  TID EGWLHTGD+
Sbjct: 385  TVVRNAELKVVDPETGRSLGYNQPGEICIRGQQIMKGYLNDEKATALTIDSEGWLHTGDV 444

Query: 574  GYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDVAAGEVPVA 395
            GYVDDD+E+FIVDRVKELIKFKGFQVPPAELE LLVSHPSIADAAVVPQKDVAAGEVPVA
Sbjct: 445  GYVDDDDEIFIVDRVKELIKFKGFQVPPAELEGLLVSHPSIADAAVVPQKDVAAGEVPVA 504

Query: 394  FVVRSNGIELTEETVKDFIAKQVVFYKKLHKVYFIHAIPKSPSGKILRKDLRAKL 230
            FVVRSNG +LTEE VK+FIAKQVVFYK+LHKVYF+HAIPKSPSGKILRKDLRAKL
Sbjct: 505  FVVRSNGFDLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKL 559


>gb|AFG30056.1| 4-coumarate: CoA ligase [Malus hybrid cultivar]
          Length = 605

 Score =  869 bits (2246), Expect = 0.0
 Identities = 435/547 (79%), Positives = 479/547 (87%), Gaps = 8/547 (1%)
 Frame = -1

Query: 1831 NHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCLISGSSNGRTYSFAETHLACKKTAA 1652
            NHVF+SKLPDIPI NHLPLHTYCF+ L EF D+ CLI GS+ G++YSF+ETHL  +KT A
Sbjct: 57   NHVFRSKLPDIPIPNHLPLHTYCFQNLPEFSDKPCLIVGST-GKSYSFSETHLISQKTGA 115

Query: 1651 GLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAITTTANPFYTSAEVFKQFTTSNAKL 1472
            GLSNLGI+KGDVIMILLQNC EFVF+F+GAS+IGA+TTTANPFYTSAE+FKQ   +NAKL
Sbjct: 116  GLSNLGIQKGDVIMILLQNCAEFVFAFMGASLIGAVTTTANPFYTSAEIFKQVKAANAKL 175

Query: 1471 IITQSQYVDKLVDFGE--------NGHKLGETYKIITVDDPPKNCLHFSIISDANETEIP 1316
            IITQSQYVDKL +           N  KLGE +K++T+DDPP+NCLHFS+ S+ANE E+P
Sbjct: 176  IITQSQYVDKLREHPSSADGADQNNYPKLGEDFKVVTIDDPPENCLHFSVFSEANEKELP 235

Query: 1315 SVFIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLTPDDVVLCVLP 1136
             V ID  DPVALPFSSGTTGLPKGVILTHKSL+TSVAQQVDGENPNLYL  DDVVLCVLP
Sbjct: 236  DVVIDAEDPVALPFSSGTTGLPKGVILTHKSLVTSVAQQVDGENPNLYLKEDDVVLCVLP 295

Query: 1135 LFHIYSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQRYKVSXXXXXXXXXXXXAKNPMV 956
            LFHI+SLNSVLLCSLRAGAGVLLM KFEIGTLLE IQRY+VS            AKNPMV
Sbjct: 296  LFHIFSLNSVLLCSLRAGAGVLLMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKNPMV 355

Query: 955  AEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQSFP 776
            AEFDLSSIRVVLSGAAPLGKELE+AL+SRVPQA+LGQGYGMTEAGPVLSMC+ FAK+  P
Sbjct: 356  AEFDLSSIRVVLSGAAPLGKELEEALKSRVPQALLGQGYGMTEAGPVLSMCMAFAKEPMP 415

Query: 775  TKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGPQIMKGYLNDVEATTTTIDIEG 596
            TKSGSCGTVVRNAELKV+D ETG SLGYNQ GEICIRG QIMKGYLNDV AT TT+D EG
Sbjct: 416  TKSGSCGTVVRNAELKVLDLETGLSLGYNQSGEICIRGSQIMKGYLNDVAATATTVDTEG 475

Query: 595  WLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDVA 416
            WLHTGD+GYVDDD E+FIVDR KELIKFKGFQVPPAELE+LL+SHPSIADAAVVPQKD A
Sbjct: 476  WLHTGDVGYVDDDNEIFIVDRAKELIKFKGFQVPPAELESLLISHPSIADAAVVPQKDDA 535

Query: 415  AGEVPVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHKVYFIHAIPKSPSGKILRKDLRA 236
            AGEVPVAFVVRSNG+ELTEE VK+FIAKQVVFYK+LHKV+F+HAIPKS SGKILRKDLRA
Sbjct: 536  AGEVPVAFVVRSNGLELTEEAVKEFIAKQVVFYKRLHKVHFVHAIPKSASGKILRKDLRA 595

Query: 235  KLGTTSP 215
            KL T +P
Sbjct: 596  KLATATP 602


>gb|AFC89538.1| 4-coumarate: coenzyme A ligase 2 [Populus tomentosa]
          Length = 569

 Score =  869 bits (2245), Expect = 0.0
 Identities = 437/566 (77%), Positives = 491/566 (86%), Gaps = 2/566 (0%)
 Frame = -1

Query: 1909 TMISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRT 1730
            ++ +VE  KP++             ++H+F+SKLPDI ISNHLPLH YCFE LS+F DR 
Sbjct: 2    SVATVEPPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENLSDFSDRP 61

Query: 1729 CLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIG 1550
            CLISGS+ G+TYSFAETHL  +K AAGLSNLGIKKGDVIM LLQNCPEFVFSF+GASMIG
Sbjct: 62   CLISGST-GKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMGASMIG 120

Query: 1549 AITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFG--ENGHKLGETYKIITVDD 1376
            A+TTTANPFYT +E+FKQF+ S AKLIITQSQYV+KL D    EN  K GE + +IT+DD
Sbjct: 121  AVTTTANPFYTQSEIFKQFSASRAKLIITQSQYVNKLGDSDCHENNQKPGEDFIVITIDD 180

Query: 1375 PPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQV 1196
            PP+NC HF+++ +ANE+E+P++ I P+DPVALPFSSGTTGLPKGVILTHKSLITSVAQQV
Sbjct: 181  PPENCRHFNVLVEANESEMPTISILPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQQV 240

Query: 1195 DGENPNLYLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQRYK 1016
            DGE PNLYL  DDVVLCVLPLFHI+SLNSVLLCSLRAG+ VLLMQKFEIG+LLE IQ++ 
Sbjct: 241  DGEIPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQKHN 300

Query: 1015 VSXXXXXXXXXXXXAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYG 836
            VS            AKNP+VA FDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYG
Sbjct: 301  VSVAAVVPPLVLALAKNPLVANFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYG 360

Query: 835  MTEAGPVLSMCLGFAKQSFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGPQ 656
            MTEAGPVLSMCL F+KQ FPTKSGSCGTVVRNAELKVIDPETG SLGYNQPGEICIRG Q
Sbjct: 361  MTEAGPVLSMCLAFSKQPFPTKSGSCGTVVRNAELKVIDPETGGSLGYNQPGEICIRGSQ 420

Query: 655  IMKGYLNDVEATTTTIDIEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEA 476
            IMKGYLND EAT  TID+EGWLHTGDIGYVDDD+E+FIVDRVKE+IKFKGFQVPPAELEA
Sbjct: 421  IMKGYLNDAEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAELEA 480

Query: 475  LLVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHKVY 296
            LLV+HPSIADAAVVPQKD  AGEVPVAFVVRS+ ++L+EE VKD+IAKQVVFYKKLHKV+
Sbjct: 481  LLVNHPSIADAAVVPQKDEVAGEVPVAFVVRSDDLDLSEEAVKDYIAKQVVFYKKLHKVF 540

Query: 295  FIHAIPKSPSGKILRKDLRAKLGTTS 218
            F+H+IPKS SGKILRKDLRAKL T++
Sbjct: 541  FVHSIPKSASGKILRKDLRAKLATST 566


>gb|ADE96997.1| p-coumarate:CoA-ligase 3 [Sorbus aucuparia]
          Length = 605

 Score =  867 bits (2240), Expect = 0.0
 Identities = 433/547 (79%), Positives = 481/547 (87%), Gaps = 8/547 (1%)
 Frame = -1

Query: 1831 NHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCLISGSSNGRTYSFAETHLACKKTAA 1652
            NHVF+SKLPDI I NHLPLHTYCF+ L EF DR CLI GS+ G++YSF+ETHL  +KT A
Sbjct: 57   NHVFRSKLPDIAIPNHLPLHTYCFQNLPEFSDRPCLIVGST-GKSYSFSETHLISQKTGA 115

Query: 1651 GLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAITTTANPFYTSAEVFKQFTTSNAKL 1472
            GLSNLGI+KGDVIMILLQNC EFVF+F+GAS+IGA+TTTANPFYT+AE+FKQ   +NAKL
Sbjct: 116  GLSNLGIQKGDVIMILLQNCAEFVFAFMGASLIGAVTTTANPFYTTAEIFKQVKAANAKL 175

Query: 1471 IITQSQYVDKLVDFGE--------NGHKLGETYKIITVDDPPKNCLHFSIISDANETEIP 1316
            IITQSQYV+KL +           N  KLGE +K++T+DDPP+NCLHFS++S+ANE E+P
Sbjct: 176  IITQSQYVNKLREHPSSADGADQNNYPKLGEDFKVVTIDDPPENCLHFSVLSEANEKELP 235

Query: 1315 SVFIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLTPDDVVLCVLP 1136
             V ID  DPVALPFSSGTTGLPKGVILTHK+L+TSVAQQVDGENPNLYL  DDVVLCVLP
Sbjct: 236  DVVIDAEDPVALPFSSGTTGLPKGVILTHKNLVTSVAQQVDGENPNLYLKEDDVVLCVLP 295

Query: 1135 LFHIYSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQRYKVSXXXXXXXXXXXXAKNPMV 956
            LFHI+S NSVLLCSLRAGAGVLLM KFEIGTLLE IQRY+VS            AKNPMV
Sbjct: 296  LFHIFSFNSVLLCSLRAGAGVLLMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKNPMV 355

Query: 955  AEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQSFP 776
            AEFDLSSIRVVLSGAAPLGKELE+AL+SRVPQA+LGQGYGMTEAGPVLSMC+ FAK+  P
Sbjct: 356  AEFDLSSIRVVLSGAAPLGKELEEALKSRVPQAVLGQGYGMTEAGPVLSMCMAFAKEPMP 415

Query: 775  TKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGPQIMKGYLNDVEATTTTIDIEG 596
            TKSGSCGTVVRNAELKV+DPETG SLGYNQPGEICIRG QIMKGYLND  AT TTID EG
Sbjct: 416  TKSGSCGTVVRNAELKVLDPETGLSLGYNQPGEICIRGFQIMKGYLNDAAATATTIDTEG 475

Query: 595  WLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDVA 416
            WLHTGD+GYVDDD+EVFIVDRVKELIKFKGFQVPPAELE+LL+SHPSIADAAVVPQ+D A
Sbjct: 476  WLHTGDVGYVDDDDEVFIVDRVKELIKFKGFQVPPAELESLLISHPSIADAAVVPQRDDA 535

Query: 415  AGEVPVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHKVYFIHAIPKSPSGKILRKDLRA 236
            AGEVPVAFVVRS+G+ELTEE VK+FIAKQVVFYK+LHKV+F+HAIPKS SGKILRKDLRA
Sbjct: 536  AGEVPVAFVVRSDGLELTEEAVKEFIAKQVVFYKRLHKVHFVHAIPKSASGKILRKDLRA 595

Query: 235  KLGTTSP 215
            KL T +P
Sbjct: 596  KLATATP 602


>ref|XP_004307864.1| PREDICTED: LOW QUALITY PROTEIN: 4-coumarate--CoA ligase 2-like
            [Fragaria vesca subsp. vesca]
          Length = 586

 Score =  864 bits (2233), Expect = 0.0
 Identities = 436/543 (80%), Positives = 478/543 (88%), Gaps = 4/543 (0%)
 Frame = -1

Query: 1831 NHVFKSKLPDIP-ISNHLPLHTYCFEKLSEFPDRTCLISGSSNGRTYSFAETHLACKKTA 1655
            +HVFKSKLPD+P ISNHLPLHTYCFE +S+F +R CLI GS+ G+TYSF+ETHL  +KT 
Sbjct: 43   HHVFKSKLPDLPNISNHLPLHTYCFENVSDFSERPCLIVGST-GKTYSFSETHLISQKTG 101

Query: 1654 AGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAITTTANPFYTSAEVFKQFTTSNAK 1475
            AGLS LGI KGDVIMILLQNC EFVF+F+GASMIGA+TTTANPFYT++E+FKQF  SNAK
Sbjct: 102  AGLSKLGITKGDVIMILLQNCAEFVFAFMGASMIGAVTTTANPFYTASEIFKQFKASNAK 161

Query: 1474 LIITQSQYVDKLVDFGENGH---KLGETYKIITVDDPPKNCLHFSIISDANETEIPSVFI 1304
            LIITQS YVDKL   G +     KLGE ++++T+DDPP+NCLHFS++S ANE+E+P V I
Sbjct: 162  LIITQSIYVDKLRHSGPDSDQYPKLGEHFQVVTIDDPPENCLHFSVLSGANESELPEVSI 221

Query: 1303 DPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLTPDDVVLCVLPLFHI 1124
            DP DPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYL  DDVVLCVLPLFHI
Sbjct: 222  DPEDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLKEDDVVLCVLPLFHI 281

Query: 1123 YSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQRYKVSXXXXXXXXXXXXAKNPMVAEFD 944
            YSLNSVLLCSLRAGA VL+M KFEIGTLLE IQRY+V              +  MVA++D
Sbjct: 282  YSLNSVLLCSLRAGAAVLVMHKFEIGTLLELIQRYRVXCGSCGATAGDSAGEESMVADYD 341

Query: 943  LSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQSFPTKSG 764
            LSSIRVVLSGAAPLGKELE+ALRSRVPQA+LGQGYGMTEAGPVLSMCL FAKQ FPTKSG
Sbjct: 342  LSSIRVVLSGAAPLGKELEEALRSRVPQAVLGQGYGMTEAGPVLSMCLAFAKQPFPTKSG 401

Query: 763  SCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGPQIMKGYLNDVEATTTTIDIEGWLHT 584
            SCGTVVRNAELKVI+PETG SLGYNQPGEICIRG QIMKGYLND  AT TT+D+EGWLHT
Sbjct: 402  SCGTVVRNAELKVIEPETGRSLGYNQPGEICIRGSQIMKGYLNDDAATATTVDVEGWLHT 461

Query: 583  GDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDVAAGEV 404
            GD+GYVDDD EVFIVDRVKELIKFKGFQVPPAELE+LLVSH SIADAAVVPQKD AAGEV
Sbjct: 462  GDVGYVDDDGEVFIVDRVKELIKFKGFQVPPAELESLLVSHQSIADAAVVPQKDDAAGEV 521

Query: 403  PVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHKVYFIHAIPKSPSGKILRKDLRAKLGT 224
            PVAFVVRSNG ELTEE VK+FIAKQVVFYK+LHKVYF+HAIPKSPSGKILRKDLRAKL  
Sbjct: 522  PVAFVVRSNGNELTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKLAA 581

Query: 223  TSP 215
             +P
Sbjct: 582  ATP 584


>gb|AAC24504.1| 4-coumarate:CoA ligase [Populus tremuloides]
          Length = 570

 Score =  861 bits (2224), Expect = 0.0
 Identities = 435/566 (76%), Positives = 488/566 (86%), Gaps = 2/566 (0%)
 Frame = -1

Query: 1909 TMISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRT 1730
            ++ +VE  KP++             ++H+F+SKLPDI ISN LPLH YCFE LS+F DR 
Sbjct: 3    SVATVEPPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNDLPLHAYCFENLSDFSDRP 62

Query: 1729 CLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIG 1550
            CLISGS+ G+TYSFAETHL  +K AAGLSNLGIKKGDVIM LLQNCPEFVFSFIGASMIG
Sbjct: 63   CLISGST-GKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFIGASMIG 121

Query: 1549 AITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFG--ENGHKLGETYKIITVDD 1376
            A+ TTANPFYT +E+FKQF+ S AKLIITQSQYV+KL D    EN  K GE + +IT+DD
Sbjct: 122  AVITTANPFYTQSEIFKQFSASRAKLIITQSQYVNKLGDSDCHENNQKPGEDFIVITIDD 181

Query: 1375 PPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQV 1196
            PP+NCLHF+++ +A+E+E+P+V I P+DPVALPFSSGTTGLPKGVILTHKSLITSVAQQV
Sbjct: 182  PPENCLHFNVLVEASESEMPTVSILPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQQV 241

Query: 1195 DGENPNLYLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQRYK 1016
            DGE PNLYL  DDVVLCVLPLFHI+SLNSVLLCSLRAG+ VLLMQKFEIG+LLE IQ++ 
Sbjct: 242  DGEIPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQKHN 301

Query: 1015 VSXXXXXXXXXXXXAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYG 836
            VS            AKNP+ A FDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYG
Sbjct: 302  VSVAAVVPPLVLALAKNPLEANFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYG 361

Query: 835  MTEAGPVLSMCLGFAKQSFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGPQ 656
            MTEAGPVLSMCL F+KQ FPTKSGSCGTVVRNAELKVIDPETG SLGYNQPGEICIRG Q
Sbjct: 362  MTEAGPVLSMCLAFSKQPFPTKSGSCGTVVRNAELKVIDPETGRSLGYNQPGEICIRGSQ 421

Query: 655  IMKGYLNDVEATTTTIDIEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEA 476
            IMKGYLND EAT  TID+EGWLHTGDIGYVDDD+E+FIVDRVKE+IKFKGFQVPPAELEA
Sbjct: 422  IMKGYLNDAEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAELEA 481

Query: 475  LLVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHKVY 296
            LLV+HPSIADAAVVPQKD  AGEVPVAFVVRS+ ++L+EE VK++IAKQVVFYKKLHKV+
Sbjct: 482  LLVNHPSIADAAVVPQKDEVAGEVPVAFVVRSDDLDLSEEAVKEYIAKQVVFYKKLHKVF 541

Query: 295  FIHAIPKSPSGKILRKDLRAKLGTTS 218
            F+H+IPKS SGKILRKDLRAKL T +
Sbjct: 542  FVHSIPKSASGKILRKDLRAKLATAT 567


>ref|NP_001237270.1| 4-coumarate:CoA ligase isoenzyme 3 [Glycine max]
            gi|4038971|gb|AAC97389.1| 4-coumarate:CoA ligase
            isoenzyme 3 [Glycine max] gi|4038973|gb|AAC97599.1|
            4-coumarate:CoA ligase isoenzyme 3 [Glycine max]
          Length = 570

 Score =  860 bits (2222), Expect = 0.0
 Identities = 425/539 (78%), Positives = 482/539 (89%)
 Frame = -1

Query: 1834 SNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRTCLISGSSNGRTYSFAETHLACKKTA 1655
            ++HVFKSKLPDIPISNHLPLH YCF+KLS+F DR CLI G +  +TY+++ETHL  +K A
Sbjct: 30   TSHVFKSKLPDIPISNHLPLHAYCFQKLSQFSDRPCLIVGPA-AKTYTYSETHLISRKIA 88

Query: 1654 AGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIGAITTTANPFYTSAEVFKQFTTSNAK 1475
            AGLSNLGI+KGDV+MILLQN  EFVFSF+ ASMIGA+ TTANPFYT+AE+FKQFT S  K
Sbjct: 89   AGLSNLGIRKGDVVMILLQNSAEFVFSFLAASMIGAVATTANPFYTAAEIFKQFTVSKTK 148

Query: 1474 LIITQSQYVDKLVDFGENGHKLGETYKIITVDDPPKNCLHFSIISDANETEIPSVFIDPN 1295
            LIITQ+ YVDKL +  ++G KLGE +K++TVDDPP+NCLHFS++S+ANE++ P V I P+
Sbjct: 149  LIITQAMYVDKLRNH-DDGAKLGEDFKVVTVDDPPENCLHFSVLSEANESDAPEVDIQPD 207

Query: 1294 DPVALPFSSGTTGLPKGVILTHKSLITSVAQQVDGENPNLYLTPDDVVLCVLPLFHIYSL 1115
            D VA+PFSSGTTGLPKGV+LTHKSL TSVAQQVDGENPNLYLT +DV+LCVLPLFHI+SL
Sbjct: 208  DAVAMPFSSGTTGLPKGVVLTHKSLTTSVAQQVDGENPNLYLTTEDVLLCVLPLFHIFSL 267

Query: 1114 NSVLLCSLRAGAGVLLMQKFEIGTLLEFIQRYKVSXXXXXXXXXXXXAKNPMVAEFDLSS 935
            NSVLLC+LRAG+ VLLMQKFEIGTLLE IQR++VS            AKNPMVA+FDLSS
Sbjct: 268  NSVLLCALRAGSAVLLMQKFEIGTLLELIQRHRVSVAMVVPPLVLALAKNPMVADFDLSS 327

Query: 934  IRVVLSGAAPLGKELEDALRSRVPQAILGQGYGMTEAGPVLSMCLGFAKQSFPTKSGSCG 755
            IR+VLSGAAPLGKEL +ALR+RVPQA+LGQGYGMTEAGPVLSMCLGFAKQ FPTKSGSCG
Sbjct: 328  IRLVLSGAAPLGKELVEALRNRVPQAVLGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCG 387

Query: 754  TVVRNAELKVIDPETGCSLGYNQPGEICIRGPQIMKGYLNDVEATTTTIDIEGWLHTGDI 575
            TVVRNAEL+V+DPETG SLGYNQPGEICIRG QIMKGYLND +AT  TID EGWLHTGD+
Sbjct: 388  TVVRNAELRVVDPETGRSLGYNQPGEICIRGQQIMKGYLNDEKATALTIDSEGWLHTGDV 447

Query: 574  GYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDVAAGEVPVA 395
            GYVD+D+E+FIVDRVKELIK+KGFQVPPAELE LLVSHPSIADAAVVPQKDVAAGEVPVA
Sbjct: 448  GYVDEDDEIFIVDRVKELIKYKGFQVPPAELEGLLVSHPSIADAAVVPQKDVAAGEVPVA 507

Query: 394  FVVRSNGIELTEETVKDFIAKQVVFYKKLHKVYFIHAIPKSPSGKILRKDLRAKLGTTS 218
            FVVRSNG +LTEE VK+FIAKQVVFYK+LHKVYF+HAIPKSPSGKILRKDLRAKL T +
Sbjct: 508  FVVRSNGFDLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKLETAA 566


>gb|EYU45376.1| hypothetical protein MIMGU_mgv1a003734mg [Mimulus guttatus]
          Length = 567

 Score =  858 bits (2218), Expect = 0.0
 Identities = 426/565 (75%), Positives = 481/565 (85%), Gaps = 1/565 (0%)
 Frame = -1

Query: 1909 TMISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRT 1730
            ++ S ETQ P++              +HVFKSKLP IPISNHLPLHTYCFE  S +PDR 
Sbjct: 3    SVASAETQNPELSSPIITQKQSSPEKSHVFKSKLPTIPISNHLPLHTYCFENFSHYPDRK 62

Query: 1729 CLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIG 1550
            CL+ G+  G++Y++AETHL C+K AAGLSNLGIKKGDV+++LLQNC EFVF+F+GASMIG
Sbjct: 63   CLLVGNGTGKSYTYAETHLICRKVAAGLSNLGIKKGDVVLVLLQNCAEFVFTFMGASMIG 122

Query: 1549 AITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFGENGHKLGETYKIITVDDPP 1370
            A+TT ANPF T+ E+FKQF  SN+++IITQ+QYVDKL + G++  K GE + +ITVDDPP
Sbjct: 123  AVTTAANPFCTANEIFKQFRASNSRMIITQAQYVDKLREGGDDSPKFGEDFSVITVDDPP 182

Query: 1369 KNCLHFSIISDANETEIPS-VFIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQVD 1193
            + CLHFS++S+ANE + P+ V ID +D V+LPFSSGTTGLPKGVILTHKSLITSVAQQVD
Sbjct: 183  EGCLHFSVLSEANENDAPAAVEIDSDDAVSLPFSSGTTGLPKGVILTHKSLITSVAQQVD 242

Query: 1192 GENPNLYLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQRYKV 1013
            GENPNLYL PDDVVLCVLPLFHIYSLNSV+LCSLRAGA VLLMQKFEIG+LLE IQR++V
Sbjct: 243  GENPNLYLKPDDVVLCVLPLFHIYSLNSVMLCSLRAGAAVLLMQKFEIGSLLELIQRHRV 302

Query: 1012 SXXXXXXXXXXXXAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYGM 833
            S            AKNPMV +FDLSSIR+VLSGAAPLGKELE AL SR+PQA+ GQGYGM
Sbjct: 303  SVAAVVPPLVLALAKNPMVDQFDLSSIRMVLSGAAPLGKELEAALLSRLPQAVFGQGYGM 362

Query: 832  TEAGPVLSMCLGFAKQSFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGPQI 653
            TEAGPVLSM   FAKQ  PTKSGSCG VVRNAELKV+DPETG S+  NQPGEICIRGPQI
Sbjct: 363  TEAGPVLSMSPSFAKQPLPTKSGSCGNVVRNAELKVVDPETGFSIPRNQPGEICIRGPQI 422

Query: 652  MKGYLNDVEATTTTIDIEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEAL 473
            MKGYLND EAT  TID+EGWLHTGDIGYVDDD++VFIVDRVKELIKFKGFQVPPAELEAL
Sbjct: 423  MKGYLNDAEATARTIDVEGWLHTGDIGYVDDDDDVFIVDRVKELIKFKGFQVPPAELEAL 482

Query: 472  LVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHKVYF 293
            LVSHPSIADAAVVPQKD AAGEVPVAFVVRSNG ELTEE VKDFI+KQVVFYK+LHKVYF
Sbjct: 483  LVSHPSIADAAVVPQKDEAAGEVPVAFVVRSNGFELTEEAVKDFISKQVVFYKRLHKVYF 542

Query: 292  IHAIPKSPSGKILRKDLRAKLGTTS 218
            +HAIPKSP+GKILRKDLRAKL   S
Sbjct: 543  VHAIPKSPAGKILRKDLRAKLAAAS 567


>ref|XP_002325815.1| 4-coumarate-CoA ligase family protein [Populus trichocarpa]
            gi|118487236|gb|ABK95446.1| unknown [Populus trichocarpa]
            gi|222862690|gb|EEF00197.1| 4-coumarate-CoA ligase family
            protein [Populus trichocarpa]
          Length = 570

 Score =  858 bits (2216), Expect = 0.0
 Identities = 433/566 (76%), Positives = 484/566 (85%), Gaps = 2/566 (0%)
 Frame = -1

Query: 1909 TMISVETQKPDVVLXXXXXXXXXTSSNHVFKSKLPDIPISNHLPLHTYCFEKLSEFPDRT 1730
            ++ +VE  KP++             ++H+F+SKLPDI ISNHLPLH YCFE LS+F DR 
Sbjct: 3    SVATVEPPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENLSDFSDRP 62

Query: 1729 CLISGSSNGRTYSFAETHLACKKTAAGLSNLGIKKGDVIMILLQNCPEFVFSFIGASMIG 1550
            CLISGS+ G+TYSFAETHL  +K AAGLSNLGIKKGDVIM LLQNCPEFVFSF+GASMIG
Sbjct: 63   CLISGST-GKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMGASMIG 121

Query: 1549 AITTTANPFYTSAEVFKQFTTSNAKLIITQSQYVDKLVDFG--ENGHKLGETYKIITVDD 1376
            A+TTT NPFYT  E+FKQF+ S AKLIITQSQ+V+KL D    EN  K  E + +IT+DD
Sbjct: 122  AVTTTVNPFYTPGEIFKQFSASRAKLIITQSQHVNKLRDSDCHENNQKPEEDFIVITIDD 181

Query: 1375 PPKNCLHFSIISDANETEIPSVFIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQV 1196
            PP+NCLHF+++ +ANE+E+P+V I P+DPVALPFSSGTTGLPKGVILTHKSLITSVAQQV
Sbjct: 182  PPENCLHFNVLVEANESEMPTVSIHPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQQV 241

Query: 1195 DGENPNLYLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGTLLEFIQRYK 1016
            DGE PNLYL  DDVVLCVLPLFHI+SLNSVLLCSLRAG+ VLLMQKFEIG+LLE IQ++ 
Sbjct: 242  DGEIPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQKHN 301

Query: 1015 VSXXXXXXXXXXXXAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYG 836
            VS            AKNPMVA FDLSSIRVVLSGAAPLGKELE+ALRSRVPQAILGQGYG
Sbjct: 302  VSVAAVVPPLVLALAKNPMVANFDLSSIRVVLSGAAPLGKELEEALRSRVPQAILGQGYG 361

Query: 835  MTEAGPVLSMCLGFAKQSFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGPQ 656
            MTEAGPVLSMCL F+KQ  PTKSGSCGTVVRNAELKVIDPETG SLG NQPGEICIRG Q
Sbjct: 362  MTEAGPVLSMCLAFSKQPLPTKSGSCGTVVRNAELKVIDPETGSSLGRNQPGEICIRGSQ 421

Query: 655  IMKGYLNDVEATTTTIDIEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEA 476
            IMKGYLND EAT   ID+EGWLHTGDIGYVDDD+E+FIVDRVKE+IKFKGFQVPPAELEA
Sbjct: 422  IMKGYLNDAEATANIIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAELEA 481

Query: 475  LLVSHPSIADAAVVPQKDVAAGEVPVAFVVRSNGIELTEETVKDFIAKQVVFYKKLHKVY 296
            LLV+HPSIADAAVVPQKD  AGEVPVAFVVRSN ++L EE VKD+IAKQVVFYKKLHKV+
Sbjct: 482  LLVNHPSIADAAVVPQKDEVAGEVPVAFVVRSNDLDLNEEAVKDYIAKQVVFYKKLHKVF 541

Query: 295  FIHAIPKSPSGKILRKDLRAKLGTTS 218
            F+H+IPKS +GKILRKDLRAKL T +
Sbjct: 542  FVHSIPKSAAGKILRKDLRAKLATAT 567