BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon23_contig00013548
         (763 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_020245684.1| diphthamide biosynthesis protein 2 isoform X...   326   e-106
ref|XP_017700254.1| PREDICTED: diphthamide biosynthesis protein ...   305   2e-99
ref|XP_008801200.1| PREDICTED: diphthamide biosynthesis protein ...   305   2e-98
ref|XP_010930233.1| PREDICTED: diphthamide biosynthesis protein ...   296   6e-95
ref|XP_018682717.1| PREDICTED: diphthamide biosynthesis protein ...   271   4e-85
ref|XP_009403226.1| PREDICTED: diphthamide biosynthesis protein ...   271   4e-85
ref|XP_020677099.1| 2-(3-amino-3-carboxypropyl)histidine synthas...   270   2e-84
ref|XP_020677098.1| 2-(3-amino-3-carboxypropyl)histidine synthas...   270   2e-84
gb|OVA08785.1| Diphthamide synthesis [Macleaya cordata]               265   1e-82
ref|XP_020106844.1| diphthamide biosynthesis protein 2 [Ananas c...   259   1e-80
gb|PKA56256.1| hypothetical protein AXF42_Ash011186 [Apostasia s...   258   2e-80
gb|OAY71771.1| Diphthamide biosynthesis protein 2 [Ananas comosus]    258   4e-80
ref|XP_020570660.1| 2-(3-amino-3-carboxypropyl)histidine synthas...   257   1e-79
ref|XP_010249074.1| PREDICTED: diphthamide biosynthesis protein ...   258   1e-79
gb|KOM44598.1| hypothetical protein LR48_Vigan05g220300 [Vigna a...   246   3e-78
ref|XP_002273627.2| PREDICTED: diphthamide biosynthesis protein ...   249   2e-76
ref|XP_021292568.1| 2-(3-amino-3-carboxypropyl)histidine synthas...   254   1e-75
ref|XP_007150748.1| hypothetical protein PHAVU_005G177500g [Phas...   247   1e-75
ref|XP_017973526.1| PREDICTED: diphthamide biosynthesis protein ...   246   1e-75
gb|PNY00193.1| diphthamide biosynthesis protein 2-like [Trifoliu...   238   3e-75

>ref|XP_020245684.1| diphthamide biosynthesis protein 2 isoform X1 [Asparagus
           officinalis]
 gb|ONK57924.1| uncharacterized protein A4U43_C09F5680 [Asparagus officinalis]
          Length = 496

 Score =  326 bits (836), Expect = e-106
 Identities = 169/240 (70%), Positives = 194/240 (80%), Gaps = 10/240 (4%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNVVRLFVMADTA 251
           MDF+ NY+I RTADFIRSR+FTR+ALQFPDELLKDSTRV+KAL+ ELG  VRLFVMADTA
Sbjct: 1   MDFELNYEILRTADFIRSRNFTRVALQFPDELLKDSTRVSKALRNELGKGVRLFVMADTA 60

Query: 252 YGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVKDCAECLSRCLLTS 431
           YGSCCVDEVAASHVDA+CVVHYGHACMSPTSTLPALFVFGKAE+DVKDC ECL RC+ + 
Sbjct: 61  YGSCCVDEVAASHVDAECVVHYGHACMSPTSTLPALFVFGKAEMDVKDCVECLYRCMSSG 120

Query: 432 ERPVLVLFGLEYAHAMQGIKYAIAD--------TSVHFAEVACSIMNPSEDITGKEDLQ- 584
           ++PVLVLFGLEYAHA+Q IK A+AD        TSVH+AEV CSIMNPS+D TGK+ LQ 
Sbjct: 121 KKPVLVLFGLEYAHALQDIKSAVADTMSLDGSSTSVHYAEVICSIMNPSQDKTGKDHLQP 180

Query: 585 -SNAIVDSSSKLFNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPMGQKMEDDLLFWIG 761
             +A V+S+ KL               ++TES  R+ LGGLTWNMP+GQ+MED LLFWIG
Sbjct: 181 HDHAGVNSNDKL--------------PSKTES--RHGLGGLTWNMPVGQRMEDSLLFWIG 224


>ref|XP_017700254.1| PREDICTED: diphthamide biosynthesis protein 2 isoform X2 [Phoenix
           dactylifera]
          Length = 422

 Score =  305 bits (782), Expect = 2e-99
 Identities = 154/241 (63%), Positives = 190/241 (78%), Gaps = 11/241 (4%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNVVRLFVMADTA 251
           MDF++NY+ISRT DFIR+R+FT++ALQFPDELLKDSTRVAKAL+ ELG  VRL+VMADTA
Sbjct: 1   MDFEANYEISRTVDFIRTRNFTKVALQFPDELLKDSTRVAKALRSELGKGVRLYVMADTA 60

Query: 252 YGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVKDCAECLSRCLLTS 431
           YGSCCVDE+ ASHVDADCVVHYGHACMSPTSTLPALFVFGKA IDV  CAE L  CL T+
Sbjct: 61  YGSCCVDEIGASHVDADCVVHYGHACMSPTSTLPALFVFGKASIDVNACAESLRHCLSTA 120

Query: 432 ERPVLVLFGLEYAHAMQGIK--------YAIADTSVHFAEVACSIMNPSED-ITGKEDLQ 584
            + ++VL+GLEYAHA++ +K        ++++++SVHFAEVACS +NPS D +T KE L 
Sbjct: 121 SKHIVVLYGLEYAHALENLKTVMAELLQFSVSNSSVHFAEVACSSINPSGDKMTEKEQLY 180

Query: 585 SNAIVDSSSKL--FNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPMGQKMEDDLLFWI 758
           S+  V    ++  + NG++ +I  S  C + E   +YSLGGL WNMP+G KMED LLFWI
Sbjct: 181 SHNGVSDIEEVHKYANGNSSLI--SVDCNKLEPCTKYSLGGLIWNMPVGHKMEDYLLFWI 238

Query: 759 G 761
           G
Sbjct: 239 G 239


>ref|XP_008801200.1| PREDICTED: diphthamide biosynthesis protein 2 isoform X1 [Phoenix
           dactylifera]
          Length = 502

 Score =  305 bits (782), Expect = 2e-98
 Identities = 154/241 (63%), Positives = 190/241 (78%), Gaps = 11/241 (4%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNVVRLFVMADTA 251
           MDF++NY+ISRT DFIR+R+FT++ALQFPDELLKDSTRVAKAL+ ELG  VRL+VMADTA
Sbjct: 1   MDFEANYEISRTVDFIRTRNFTKVALQFPDELLKDSTRVAKALRSELGKGVRLYVMADTA 60

Query: 252 YGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVKDCAECLSRCLLTS 431
           YGSCCVDE+ ASHVDADCVVHYGHACMSPTSTLPALFVFGKA IDV  CAE L  CL T+
Sbjct: 61  YGSCCVDEIGASHVDADCVVHYGHACMSPTSTLPALFVFGKASIDVNACAESLRHCLSTA 120

Query: 432 ERPVLVLFGLEYAHAMQGIK--------YAIADTSVHFAEVACSIMNPSED-ITGKEDLQ 584
            + ++VL+GLEYAHA++ +K        ++++++SVHFAEVACS +NPS D +T KE L 
Sbjct: 121 SKHIVVLYGLEYAHALENLKTVMAELLQFSVSNSSVHFAEVACSSINPSGDKMTEKEQLY 180

Query: 585 SNAIVDSSSKL--FNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPMGQKMEDDLLFWI 758
           S+  V    ++  + NG++ +I  S  C + E   +YSLGGL WNMP+G KMED LLFWI
Sbjct: 181 SHNGVSDIEEVHKYANGNSSLI--SVDCNKLEPCTKYSLGGLIWNMPVGHKMEDYLLFWI 238

Query: 759 G 761
           G
Sbjct: 239 G 239


>ref|XP_010930233.1| PREDICTED: diphthamide biosynthesis protein 2 [Elaeis guineensis]
          Length = 503

 Score =  296 bits (759), Expect = 6e-95
 Identities = 151/241 (62%), Positives = 185/241 (76%), Gaps = 11/241 (4%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNVVRLFVMADTA 251
           MDF+SNY+ISRTADFIR+R+FT++ALQFPDELLK+STRVAKAL+ ELG  VRL+VMAD A
Sbjct: 1   MDFESNYEISRTADFIRTRNFTKVALQFPDELLKESTRVAKALRNELGKGVRLYVMADAA 60

Query: 252 YGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVKDCAECLSRCLLTS 431
           YGSCCVDE+ ASHVDADCVVHYGHACMSPTSTL ALFVFGKA IDV  CA+ L  CL T+
Sbjct: 61  YGSCCVDEIGASHVDADCVVHYGHACMSPTSTLLALFVFGKASIDVNACAKSLRHCLSTA 120

Query: 432 ERPVLVLFGLEYAHAMQGIKYAIAD--------TSVHFAEVACSIMNPSED-ITGKEDLQ 584
            +P+LVL+GLEYAHA++ +K  +A+        + VHFAEVACS +NPS D +T K+ L 
Sbjct: 121 NKPILVLYGLEYAHALENLKTVVAELLQLSVSNSGVHFAEVACSSINPSGDKMTDKKQLY 180

Query: 585 SNAIVDSSSKL--FNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPMGQKMEDDLLFWI 758
           S+  V  + ++  + +G+T +I  S  C   E   +YSLGGL W MP+G KMED LL WI
Sbjct: 181 SHNGVSDNEEVHKYEDGNTSLI--SVNCNTLEPCTKYSLGGLIWTMPVGHKMEDYLLLWI 238

Query: 759 G 761
           G
Sbjct: 239 G 239


>ref|XP_018682717.1| PREDICTED: diphthamide biosynthesis protein 2 isoform X2 [Musa
           acuminata subsp. malaccensis]
          Length = 506

 Score =  271 bits (694), Expect = 4e-85
 Identities = 134/241 (55%), Positives = 177/241 (73%), Gaps = 11/241 (4%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNVVRLFVMADTA 251
           MDF+ NY+ISRT D++R+ +FTR+ALQFPDELLKDS RVAKAL+ ELG  VRLFVMAD A
Sbjct: 1   MDFELNYEISRTVDYLRAGNFTRVALQFPDELLKDSPRVAKALRSELGAGVRLFVMADAA 60

Query: 252 YGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVKDCAECLSRCLLTS 431
           YGSCCVDE+ ASHVDA+CVVHYGHACMSPTSTLPA+FVFGKA ID+KDCA+ +  CL ++
Sbjct: 61  YGSCCVDEIGASHVDAECVVHYGHACMSPTSTLPAMFVFGKASIDIKDCADLIGHCLSST 120

Query: 432 ERPVLVLFGLEYAHAMQGIKYAIADT----------SVHFAEVACSIMNPSEDITGKEDL 581
            +P+LVL+GLEY+HA+  +K  +A++           V +++V  S++NPS+D T ++D 
Sbjct: 121 NKPILVLYGLEYSHALMHLKAVVAESLVLSHSNWGPMVKYSDVIGSVINPSQDCTTEKDQ 180

Query: 582 QSNAIVDSSSKLFNNG-DTQMIPKSDQCTETESGMRYSLGGLTWNMPMGQKMEDDLLFWI 758
             +     ++  F +G D + I K       E+G  + LGGLTW++P   K+ED LLFWI
Sbjct: 181 IRSLNGSITNAGFTSGRDEKKILKFGNGRRMENGTCFELGGLTWSIPSDHKIEDYLLFWI 240

Query: 759 G 761
           G
Sbjct: 241 G 241


>ref|XP_009403226.1| PREDICTED: diphthamide biosynthesis protein 2 isoform X1 [Musa
           acuminata subsp. malaccensis]
          Length = 508

 Score =  271 bits (694), Expect = 4e-85
 Identities = 134/241 (55%), Positives = 177/241 (73%), Gaps = 11/241 (4%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNVVRLFVMADTA 251
           MDF+ NY+ISRT D++R+ +FTR+ALQFPDELLKDS RVAKAL+ ELG  VRLFVMAD A
Sbjct: 1   MDFELNYEISRTVDYLRAGNFTRVALQFPDELLKDSPRVAKALRSELGAGVRLFVMADAA 60

Query: 252 YGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVKDCAECLSRCLLTS 431
           YGSCCVDE+ ASHVDA+CVVHYGHACMSPTSTLPA+FVFGKA ID+KDCA+ +  CL ++
Sbjct: 61  YGSCCVDEIGASHVDAECVVHYGHACMSPTSTLPAMFVFGKASIDIKDCADLIGHCLSST 120

Query: 432 ERPVLVLFGLEYAHAMQGIKYAIADT----------SVHFAEVACSIMNPSEDITGKEDL 581
            +P+LVL+GLEY+HA+  +K  +A++           V +++V  S++NPS+D T ++D 
Sbjct: 121 NKPILVLYGLEYSHALMHLKAVVAESLVLSHSNWGPMVKYSDVIGSVINPSQDCTTEKDQ 180

Query: 582 QSNAIVDSSSKLFNNG-DTQMIPKSDQCTETESGMRYSLGGLTWNMPMGQKMEDDLLFWI 758
             +     ++  F +G D + I K       E+G  + LGGLTW++P   K+ED LLFWI
Sbjct: 181 IRSLNGSITNAGFTSGRDEKKILKFGNGRRMENGTCFELGGLTWSIPSDHKIEDYLLFWI 240

Query: 759 G 761
           G
Sbjct: 241 G 241


>ref|XP_020677099.1| 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 isoform X2
           [Dendrobium catenatum]
 gb|PKU65790.1| hypothetical protein MA16_Dca027241 [Dendrobium catenatum]
          Length = 498

 Score =  270 bits (689), Expect = 2e-84
 Identities = 137/238 (57%), Positives = 172/238 (72%), Gaps = 8/238 (3%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNVVRLFVMADTA 251
           MD +SNY+I RTAD+IRS +F R+ALQFPDELLKDSTRVA+AL+ ELG  VRLFVMADTA
Sbjct: 1   MDLESNYEIHRTADYIRSNNFARVALQFPDELLKDSTRVARALRNELGRGVRLFVMADTA 60

Query: 252 YGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVKDCAECLSRCLLTS 431
           YGSCCVDEV A+HVDA+CVVHYGHACMSPT+TLPA FVFGKA IDV DC + L   L ++
Sbjct: 61  YGSCCVDEVGAAHVDAECVVHYGHACMSPTTTLPAFFVFGKASIDVSDCVQSLHHHLSSN 120

Query: 432 ERPVLVLFGLEYAHAMQGIKYAIA--------DTSVHFAEVACSIMNPSEDITGKEDLQS 587
            +P++VLFGLEYAHA+Q IK A+A           + +AEV  S++NPS D + + + Q 
Sbjct: 121 NKPIMVLFGLEYAHALQDIKSAMAMSLPSTGFSPMIQYAEVTSSVVNPSGD-SDEINGQD 179

Query: 588 NAIVDSSSKLFNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPMGQKMEDDLLFWIG 761
             +VD      N  +     KS++C++  +  RY LGGL W++PM QK+   LL WIG
Sbjct: 180 TKVVD-----LNCDEKDKTSKSEKCSKLVTDTRYDLGGLKWSIPMDQKINAYLLLWIG 232


>ref|XP_020677098.1| 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 isoform X1
           [Dendrobium catenatum]
          Length = 499

 Score =  270 bits (689), Expect = 2e-84
 Identities = 137/238 (57%), Positives = 172/238 (72%), Gaps = 8/238 (3%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNVVRLFVMADTA 251
           MD +SNY+I RTAD+IRS +F R+ALQFPDELLKDSTRVA+AL+ ELG  VRLFVMADTA
Sbjct: 1   MDLESNYEIHRTADYIRSNNFARVALQFPDELLKDSTRVARALRNELGRGVRLFVMADTA 60

Query: 252 YGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVKDCAECLSRCLLTS 431
           YGSCCVDEV A+HVDA+CVVHYGHACMSPT+TLPA FVFGKA IDV DC + L   L ++
Sbjct: 61  YGSCCVDEVGAAHVDAECVVHYGHACMSPTTTLPAFFVFGKASIDVSDCVQSLHHHLSSN 120

Query: 432 ERPVLVLFGLEYAHAMQGIKYAIA--------DTSVHFAEVACSIMNPSEDITGKEDLQS 587
            +P++VLFGLEYAHA+Q IK A+A           + +AEV  S++NPS D + + + Q 
Sbjct: 121 NKPIMVLFGLEYAHALQDIKSAMAMSLPSTGFSPMIQYAEVTSSVVNPSGD-SDEINGQD 179

Query: 588 NAIVDSSSKLFNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPMGQKMEDDLLFWIG 761
             +VD      N  +     KS++C++  +  RY LGGL W++PM QK+   LL WIG
Sbjct: 180 TKVVD-----LNCDEKDKTSKSEKCSKLVTDTRYDLGGLKWSIPMDQKINAYLLLWIG 232


>gb|OVA08785.1| Diphthamide synthesis [Macleaya cordata]
          Length = 510

 Score =  265 bits (677), Expect = 1e-82
 Identities = 139/249 (55%), Positives = 175/249 (70%), Gaps = 19/249 (7%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNV---------- 221
           MDF SNY+I+RT +FIRS++FTR+ALQFPDELLKDS RV   L++EL ++          
Sbjct: 1   MDFASNYEIARTTNFIRSKNFTRVALQFPDELLKDSIRVVSELREELRSLTTESDGREDA 60

Query: 222 --VRLFVMADTAYGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVKD 395
             + L+VMADT YGSCCVDEV ASHVDADCVVHYGH C+SPTSTLPALFVFGK  I+V D
Sbjct: 61  RDIGLYVMADTTYGSCCVDEVGASHVDADCVVHYGHTCLSPTSTLPALFVFGKGSINVSD 120

Query: 396 CAECLSRCLLTSERPVLVLFGLEYAHAMQGIKYAI------ADTSVHFAEVACSIMNPSE 557
           C + LS CL TS++PVLVL+GLEYAHA+  IK +       ++  +HFA+V CS++NPSE
Sbjct: 121 CVKSLSECLSTSDKPVLVLYGLEYAHAIHSIKESSTLCGPKSNLGLHFADVVCSVINPSE 180

Query: 558 DI-TGKEDLQSNAIVDSSSKLFNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPMGQKM 734
           +  T  ++L S+   DS++    N   QM    D       G +YS+GGLTWN+P G KM
Sbjct: 181 NYETPSKELASS---DSTANGVYNDCKQMGSNLD-------GTKYSIGGLTWNLPHGHKM 230

Query: 735 EDDLLFWIG 761
           ED +LFW G
Sbjct: 231 EDYMLFWFG 239


>ref|XP_020106844.1| diphthamide biosynthesis protein 2 [Ananas comosus]
          Length = 498

 Score =  259 bits (663), Expect = 1e-80
 Identities = 137/238 (57%), Positives = 173/238 (72%), Gaps = 8/238 (3%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNVVRLFVMADTA 251
           MDF+S+YDI RT D+IRS  ++R+ LQFPDELL+DST VAKAL+ ELG  VRL+VMADTA
Sbjct: 1   MDFESSYDIPRTVDYIRSIGYSRVVLQFPDELLRDSTSVAKALRSELGKGVRLYVMADTA 60

Query: 252 YGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVKDCAECLSRCLLTS 431
           Y SCCVDE+ ASHVDA+CVVHYGHACMSPTSTLPALFVFGKA I+   CA+ L++CL T+
Sbjct: 61  YNSCCVDEIGASHVDAECVVHYGHACMSPTSTLPALFVFGKAPINGNACADLLNQCLSTT 120

Query: 432 ERPVLVLFGLEYAHAMQGIKYAIADTS--------VHFAEVACSIMNPSEDITGKEDLQS 587
           E+PVLVL+GLEYAHAM+ +K  + ++S        VHFAEV+ S ++P   +  K D + 
Sbjct: 121 EKPVLVLYGLEYAHAMENLKGMVTNSSNLRYGSSTVHFAEVSFSSIDP--PVKEKADKEQ 178

Query: 588 NAIVDSSSKLFNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPMGQKMEDDLLFWIG 761
              +D    + N GD  +   S+  +  E G +Y LGGLTW++    KMED LLFWIG
Sbjct: 179 ---LDFHGNIVNGGDKII---SEWNSHNEVG-KYFLGGLTWSIAKDHKMEDFLLFWIG 229


>gb|PKA56256.1| hypothetical protein AXF42_Ash011186 [Apostasia shenzhenica]
          Length = 472

 Score =  258 bits (660), Expect = 2e-80
 Identities = 135/238 (56%), Positives = 161/238 (67%), Gaps = 8/238 (3%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNVVRLFVMADTA 251
           MD  SNY+ISRTADFIRSRSFTR+ALQFPD+LL+DST+VA+AL+ ELG  VRLFVMADTA
Sbjct: 1   MDLLSNYEISRTADFIRSRSFTRVALQFPDDLLRDSTKVARALRSELGRRVRLFVMADTA 60

Query: 252 YGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVKDCAECLSRCLLTS 431
           YGSCCVDEV A+HVDA+CV+HYGHACMSPT+TLPA FVFGKA IDV DC + L  CL T+
Sbjct: 61  YGSCCVDEVGAAHVDAECVIHYGHACMSPTTTLPAFFVFGKATIDVDDCVQSLHHCLSTN 120

Query: 432 ERPVLVLFGLEYAHAMQGIKYAIADT--------SVHFAEVACSIMNPSEDITGKEDLQS 587
            RP+LVL GLEY HA+Q IK A++           V +AEV  +I+NPSED   ++    
Sbjct: 121 NRPILVLLGLEYLHALQDIKAAMSPRLQCTGSGLMVTYAEVTSTIINPSEDCDRRKG--- 177

Query: 588 NAIVDSSSKLFNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPMGQKMEDDLLFWIG 761
                                            + LGGLTW++P G KME+ LLFWIG
Sbjct: 178 ---------------------------------FFLGGLTWSIPTGHKMEEYLLFWIG 202


>gb|OAY71771.1| Diphthamide biosynthesis protein 2 [Ananas comosus]
          Length = 498

 Score =  258 bits (660), Expect = 4e-80
 Identities = 137/238 (57%), Positives = 173/238 (72%), Gaps = 8/238 (3%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNVVRLFVMADTA 251
           MDF+S+YDI RT D+I SR ++R+ LQFPDELL+DST VAKAL+ ELG  VRL+VMADTA
Sbjct: 1   MDFESSYDIPRTVDYICSRGYSRVVLQFPDELLRDSTSVAKALRCELGKGVRLYVMADTA 60

Query: 252 YGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVKDCAECLSRCLLTS 431
           Y SCCVDE+ ASHVDA+CVVHYGHACMSPTSTLPALFVFGKA I+   CA+ L++CL T+
Sbjct: 61  YNSCCVDEIGASHVDAECVVHYGHACMSPTSTLPALFVFGKAPINGNACADLLNQCLSTT 120

Query: 432 ERPVLVLFGLEYAHAMQGIKYAIADTS--------VHFAEVACSIMNPSEDITGKEDLQS 587
           E+PVLVL+GLEYAHAM+ +K  + ++S        VHFAEV+ S ++P   +  K D + 
Sbjct: 121 EKPVLVLYGLEYAHAMENLKGMVTNSSNLRYGSSTVHFAEVSFSSIDP--PVKEKADKEQ 178

Query: 588 NAIVDSSSKLFNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPMGQKMEDDLLFWIG 761
              +D    + N GD  +   S+  +  E G +Y LGGLTW++    KMED LLFWIG
Sbjct: 179 ---LDFHGNIVNGGDKII---SEWNSHNEVG-KYFLGGLTWSITKDHKMEDFLLFWIG 229


>ref|XP_020570660.1| 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 isoform X1
           [Phalaenopsis equestris]
          Length = 502

 Score =  257 bits (657), Expect = 1e-79
 Identities = 126/238 (52%), Positives = 170/238 (71%), Gaps = 8/238 (3%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNVVRLFVMADTA 251
           MD +S Y+I RTA+FIRS +F+R+ALQFPDELL+DST VA+AL+ ELG  VRLFVMADTA
Sbjct: 1   MDLESKYEILRTANFIRSNNFSRVALQFPDELLRDSTSVARALRNELGRGVRLFVMADTA 60

Query: 252 YGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVKDCAECLSRCLLTS 431
           YGSCCVDE+ A+H+DADCVVH+GHACMSP +TLPA FVFGK+ IDV  C + L   L ++
Sbjct: 61  YGSCCVDEIGAAHIDADCVVHFGHACMSPLTTLPAFFVFGKSSIDVLVCVQSLHHNLSSN 120

Query: 432 ERPVLVLFGLEYAHAMQGIKYAIADT--------SVHFAEVACSIMNPSEDITGKEDLQS 587
            +P+L+LFGLEYAHA++ IK  +  +         V +AEV  SI+NPS+D  G     +
Sbjct: 121 NKPILILFGLEYAHALEDIKAKMVSSLPSAGFSQIVRYAEVTSSIINPSDDNDGINGRNT 180

Query: 588 NAIVDSSSKLFNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPMGQKMEDDLLFWIG 761
             +V       N G  +   KS++C++  +G RY LGGL W++P+ +K++D ++ WIG
Sbjct: 181 KVVV------LNCGHKEKTLKSEKCSKLVTGTRYGLGGLEWSIPVDKKIDDYIMVWIG 232


>ref|XP_010249074.1| PREDICTED: diphthamide biosynthesis protein 2 [Nelumbo nucifera]
          Length = 516

 Score =  258 bits (658), Expect = 1e-79
 Identities = 139/256 (54%), Positives = 171/256 (66%), Gaps = 26/256 (10%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNV---------- 221
           MDF SNY+ISRTA FIRSR+FTR+ALQFPDELLKDST+V  AL+ EL ++          
Sbjct: 1   MDFSSNYEISRTAKFIRSRNFTRVALQFPDELLKDSTKVVSALRDELRSLSESDAEGNRD 60

Query: 222 ---VRLFVMADTAYGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVK 392
              V LFVMADT YG+CCVDEV ASHVDA+CVVHYGH C+SPTSTLPALFVFG A I+  
Sbjct: 61  YRDVGLFVMADTTYGNCCVDEVGASHVDAECVVHYGHTCLSPTSTLPALFVFGNASINSN 120

Query: 393 DCAECLSRCLLTSERPVLVLFGLEYAHAMQGIKYAI-----------ADTSVHFAEVACS 539
           DCA+ LS CLLT+E+P++VLFGLEYAHA+  IK  +            + ++ +AEV CS
Sbjct: 121 DCAKSLSNCLLTNEKPIVVLFGLEYAHAIHHIKEIMEVESSRLCGSKENKALCYAEVICS 180

Query: 540 IMNPSE--DITGKEDLQSNAIVDSSSKLFNNGDTQMIPKSDQCTETESGMRYSLGGLTWN 713
           IM+P E     G++       + S      NGD      S Q   T  G +Y+LGGLTW 
Sbjct: 181 IMDPEERFKTPGRQ-------LGSFDSYTANGD---CANSGQMNSTGCGNKYTLGGLTWK 230

Query: 714 MPMGQKMEDDLLFWIG 761
           +P G KM+D ++FWIG
Sbjct: 231 LPEGHKMDDYMIFWIG 246


>gb|KOM44598.1| hypothetical protein LR48_Vigan05g220300 [Vigna angularis]
          Length = 253

 Score =  246 bits (627), Expect = 3e-78
 Identities = 132/253 (52%), Positives = 163/253 (64%), Gaps = 23/253 (9%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNV---------- 221
           MDF+SNYDI+ +A FI + +F R+ALQFPD LLKDSTRV  AL+K L ++          
Sbjct: 3   MDFESNYDIAASALFIHTHNFNRVALQFPDNLLKDSTRVVTALRKRLQSLKKIDVSESGY 62

Query: 222 ---VRLFVMADTAYGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVK 392
              V LFVMADTAYGSCCVDEV ASH++ DCV+HYGH C SPT+TLP+ FVFGKA I V 
Sbjct: 63  EADVGLFVMADTAYGSCCVDEVGASHINVDCVIHYGHTCFSPTTTLPSFFVFGKASICVA 122

Query: 393 DCAECLSRCLLTSERPVLVLFGLEYAHAMQGIKYAIADTS----------VHFAEVACSI 542
           DC E +S+  LT+ +PV+VLFGLEYAH+MQ IK A+ ++S          VHFA+V  S+
Sbjct: 123 DCVESMSKYALTNSKPVMVLFGLEYAHSMQQIKEALLESSMSCRIDPKPEVHFADVPSSV 182

Query: 543 MNPSEDITGKEDLQSNAIVDSSSKLFNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPM 722
           M PS+DI   + LQ  A   +                      ESG  YS+GGLTW +P 
Sbjct: 183 MFPSKDIKKIKGLQELACGCNG---------------------ESGTTYSIGGLTWKLPQ 221

Query: 723 GQKMEDDLLFWIG 761
           GQ M+D LLFWIG
Sbjct: 222 GQSMDDYLLFWIG 234


>ref|XP_002273627.2| PREDICTED: diphthamide biosynthesis protein 2 isoform X1 [Vitis
           vinifera]
 emb|CBI24661.3| unnamed protein product, partial [Vitis vinifera]
          Length = 506

 Score =  249 bits (635), Expect = 2e-76
 Identities = 134/251 (53%), Positives = 164/251 (65%), Gaps = 21/251 (8%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKEL----------GNV 221
           M+ +SNY+I+ TADFIRSR+FTR+ALQFPDELLKD+TRV KAL+  L          G  
Sbjct: 1   MELESNYEIALTADFIRSRNFTRVALQFPDELLKDATRVVKALRDRLESPSESGSGDGKE 60

Query: 222 VRLFVMADTAYGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVKDCA 401
           V LFVMADT YGSCCVDEV ASH++ADCVVHYGH C+SPTS LPA FVFGKA I + +CA
Sbjct: 61  VGLFVMADTTYGSCCVDEVGASHINADCVVHYGHTCLSPTSNLPAFFVFGKAPISISNCA 120

Query: 402 ECLSRCLLTSERPVLVLFGLEYAHAMQGIKYAI-----------ADTSVHFAEVACSIMN 548
           E LS   LT  + ++VLFGLEY+HA++ IK A+           ++   HFAEV CS+MN
Sbjct: 121 ENLSTYALTQGKHIMVLFGLEYSHAIEMIKEALMLESSKLSGSTSNLEFHFAEVMCSVMN 180

Query: 549 PSEDITGKEDLQSNAIVDSSSKLFNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPMGQ 728
           PS+D T      SN  + S      N          +     +G RYS+GGL WN+P G 
Sbjct: 181 PSKDCT-----TSNGHLGSVGSFTAN----------EGFGKATGTRYSIGGLAWNLPEGH 225

Query: 729 KMEDDLLFWIG 761
           +MED LL WIG
Sbjct: 226 RMEDYLLVWIG 236


>ref|XP_021292568.1| 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 [Herrania
            umbratica]
          Length = 839

 Score =  254 bits (650), Expect = 1e-75
 Identities = 133/239 (55%), Positives = 167/239 (69%), Gaps = 8/239 (3%)
 Frame = +3

Query: 69   SMDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELG--NVVRLFVMA 242
            +MD ++N++ISRTA+FIRSR+FTR+ALQFPD+LLK ST+V  +L+K+LG  N V LFVMA
Sbjct: 349  AMDLETNFEISRTAEFIRSRNFTRVALQFPDDLLKHSTKVVTSLRKKLGSGNKVGLFVMA 408

Query: 243  DTAYGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVKDCAECLSRCL 422
            DTAYGSCCVDEV ASH+DADCVVHYGH C+SPTSTLP+  VFGKA I+V  C E LS   
Sbjct: 409  DTAYGSCCVDEVGASHIDADCVVHYGHTCLSPTSTLPSFCVFGKASINVSSCVEKLSSFF 468

Query: 423  LTSERPVLVLFGLEYAHAMQGIKYAI------ADTSVHFAEVACSIMNPSEDITGKEDLQ 584
             T  RP +VL+GLEYAHA+  IK A+      A   + FA+V CSI+NP ED     + +
Sbjct: 469  WTDGRPTVVLYGLEYAHAISQIKEALVEATPRAKVELQFADVMCSIINPLED-----NRR 523

Query: 585  SNAIVDSSSKLFNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPMGQKMEDDLLFWIG 761
            SN ++       NN            +  E+G RYSLGGL W++P GQ+MED +L WIG
Sbjct: 524  SNGLLGLLGSCTNNS-----------SGAETGTRYSLGGLIWDLPEGQRMEDYMLLWIG 571


>ref|XP_007150748.1| hypothetical protein PHAVU_005G177500g [Phaseolus vulgaris]
 gb|ESW22742.1| hypothetical protein PHAVU_005G177500g [Phaseolus vulgaris]
          Length = 501

 Score =  247 bits (630), Expect = 1e-75
 Identities = 130/253 (51%), Positives = 165/253 (65%), Gaps = 23/253 (9%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNV---------- 221
           MDF+SNYDI+ +A FI + +FTR+ALQFPD LLKDSTRV  AL+K L ++          
Sbjct: 1   MDFESNYDIAASAQFIHTHNFTRVALQFPDNLLKDSTRVVTALRKRLQSLKEIDATESGH 60

Query: 222 ---VRLFVMADTAYGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVK 392
              V LFVMADTAYGSCCVDEV ASH++ADCV+HYGH C SPT+TLP+ FVFGKA I + 
Sbjct: 61  ETDVGLFVMADTAYGSCCVDEVGASHINADCVIHYGHTCFSPTTTLPSFFVFGKASICIA 120

Query: 393 DCAECLSRCLLTSERPVLVLFGLEYAHAMQGIKYAIADTS----------VHFAEVACSI 542
           DC E +S+  LT+ +P++V+FGLEYAH+MQ IK A+ ++S          VHFA+   S+
Sbjct: 121 DCVESMSKYALTNSKPIMVVFGLEYAHSMQQIKEALLESSVSCRFDPKPDVHFADFPSSV 180

Query: 543 MNPSEDITGKEDLQSNAIVDSSSKLFNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPM 722
           M PS+DI   + LQ  A                      C + E+G  YS+GGLTW +P 
Sbjct: 181 MFPSKDIKKIKGLQEPA--------------------SGCND-ENGTTYSIGGLTWKLPE 219

Query: 723 GQKMEDDLLFWIG 761
           GQ M+D LLFWIG
Sbjct: 220 GQSMDDYLLFWIG 232


>ref|XP_017973526.1| PREDICTED: diphthamide biosynthesis protein 2 [Theobroma cacao]
          Length = 490

 Score =  246 bits (629), Expect = 1e-75
 Identities = 130/238 (54%), Positives = 163/238 (68%), Gaps = 8/238 (3%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELG--NVVRLFVMAD 245
           MD ++N++ISRTA+FI SR+FTR+ALQFPD+LLK ST+V  +L+ +LG  N V LFVMAD
Sbjct: 1   MDLETNFEISRTAEFILSRNFTRVALQFPDDLLKHSTKVVNSLRNKLGSGNKVGLFVMAD 60

Query: 246 TAYGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVKDCAECLSRCLL 425
           TAYGSCCVDEV ASH+DADCV+HYGH C+SPTSTLP+  VFGKA I V  C E LS    
Sbjct: 61  TAYGSCCVDEVGASHIDADCVIHYGHTCLSPTSTLPSFCVFGKASISVFSCVEKLSSFFW 120

Query: 426 TSERPVLVLFGLEYAHAMQGIKYAI------ADTSVHFAEVACSIMNPSEDITGKEDLQS 587
           T+ RP +VL+GLEYAHA+  IK A+      A   + FA+V CSI+NP ED       +S
Sbjct: 121 TNCRPTVVLYGLEYAHAISQIKEALVEATPKAKVELQFADVMCSIINPLED-----HRRS 175

Query: 588 NAIVDSSSKLFNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPMGQKMEDDLLFWIG 761
           N ++       +N            +  E+G RYSLGGL W++P GQKMED +L WIG
Sbjct: 176 NGLLGHLGSCTSNS-----------SGAETGSRYSLGGLIWDLPEGQKMEDYMLLWIG 222


>gb|PNY00193.1| diphthamide biosynthesis protein 2-like [Trifolium pratense]
          Length = 256

 Score =  238 bits (607), Expect = 3e-75
 Identities = 121/252 (48%), Positives = 164/252 (65%), Gaps = 22/252 (8%)
 Frame = +3

Query: 72  MDFDSNYDISRTADFIRSRSFTRLALQFPDELLKDSTRVAKALKKELGNV---------- 221
           MDF+SNYDI+ T++FIR+ +FTR+ALQFPD+LLKDSTRV   L+K L ++          
Sbjct: 1   MDFESNYDIAATSEFIRTHNFTRVALQFPDDLLKDSTRVVAVLRKRLQSLRESDITVSGE 60

Query: 222 ---VRLFVMADTAYGSCCVDEVAASHVDADCVVHYGHACMSPTSTLPALFVFGKAEIDVK 392
              + L+VMADTAYGSCCVDEV ASH++ADCV+HYGH C SPT+ LPA FVFGKA I + 
Sbjct: 61  ETEIGLYVMADTAYGSCCVDEVGASHINADCVIHYGHTCFSPTTALPAFFVFGKASIGIA 120

Query: 393 DCAECLSRCLLTSERPVLVLFGLEYAHAMQGIKYAIADTS---------VHFAEVACSIM 545
           DC E +S+  LT+ +P++VLFGLEYAH++Q I+  + ++S         VHFA+V    M
Sbjct: 121 DCVESMSQYALTNSKPIMVLFGLEYAHSIQQIRKELLESSTCKSDLKSEVHFADVPSPYM 180

Query: 546 NPSEDITGKEDLQSNAIVDSSSKLFNNGDTQMIPKSDQCTETESGMRYSLGGLTWNMPMG 725
            PS+D+     +Q  A                   ++  ++  +G+ Y++GGLTW +P G
Sbjct: 181 FPSKDMKKLSGIQEEACGCG---------------NNSSSDGANGIIYNIGGLTWKLPEG 225

Query: 726 QKMEDDLLFWIG 761
           Q MED  LFWIG
Sbjct: 226 QSMEDYSLFWIG 237