BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon22_contig00022735
         (1435 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_008783492.1| PREDICTED: S-adenosyl-L-methionine-dependent...   740   0.0  
ref|XP_008783491.1| PREDICTED: S-adenosyl-L-methionine-dependent...   738   0.0  
ref|XP_010942945.1| PREDICTED: S-adenosyl-L-methionine-dependent...   734   0.0  
ref|NP_001347253.1| uncharacterized protein LOC111828519 [Oryza ...   721   0.0  
dbj|BAF13343.1| Os03g0775300 [Oryza sativa Japonica Group] >gi|9...   729   0.0  
ref|XP_015631211.1| PREDICTED: S-adenosyl-L-methionine-dependent...   729   0.0  
ref|XP_020704408.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   721   0.0  
gb|KQK86877.1| hypothetical protein SETIT_034607mg [Setaria ital...   719   0.0  
ref|NP_001347248.1| uncharacterized protein LOC100273754 [Zea ma...   719   0.0  
ref|XP_009399072.1| PREDICTED: S-adenosyl-L-methionine-dependent...   719   0.0  
ref|NP_001347259.1| uncharacterized LOC111828523 [Sorghum bicolor]    718   0.0  
ref|NP_001347566.1| uncharacterized protein LOC111946219 [Setari...   719   0.0  
ref|XP_020180383.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   717   0.0  
dbj|BAK06688.1| predicted protein [Hordeum vulgare subsp. vulgare]    716   0.0  
ref|XP_020092095.1| S-adenosyl-L-methionine-dependent tRNA 4-dem...   715   0.0  
gb|OAY80972.1| S-adenosyl-L-methionine-dependent tRNA 4-demethyl...   715   0.0  
ref|XP_014752193.1| PREDICTED: S-adenosyl-L-methionine-dependent...   713   0.0  
gb|KZV28868.1| S-adenosyl-L-methionine-dependent tRNA 4-demethyl...   713   0.0  
ref|XP_017981378.1| PREDICTED: S-adenosyl-L-methionine-dependent...   710   0.0  
gb|EOY14757.1| Flavodoxin family protein / radical SAM domain-co...   710   0.0  

>ref|XP_008783492.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like isoform X1 [Phoenix dactylifera]
          Length = 648

 Score =  740 bits (1910), Expect = 0.0
 Identities = 351/405 (86%), Positives = 375/405 (92%)
 Frame = -1

Query: 1435 SRKLVASLVNGENGVKEKSFVNGENGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTK 1256
            SRK   +L NG          NG+NG REMVTP+IR SL+KQGYKI+GSHSGVK+CRWTK
Sbjct: 248  SRKSTGALSNGGQ--------NGDNGLREMVTPIIRTSLEKQGYKIVGSHSGVKLCRWTK 299

Query: 1255 SQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVI 1076
            SQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVI
Sbjct: 300  SQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVI 359

Query: 1075 VNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRH 896
            VNAAIDQHTKMIKQMKGVPGV QE+L EGLSP+HCALSLVGEPIMYPEIN+LIDELH RH
Sbjct: 360  VNAAIDQHTKMIKQMKGVPGVKQEQLLEGLSPKHCALSLVGEPIMYPEINSLIDELHSRH 419

Query: 895  ISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRD 716
            ISTFLVTNAQFPD+IK L+P+TQLYVSVDAATKDSLKAIDRPLF DFWERFVDSLKALRD
Sbjct: 420  ISTFLVTNAQFPDRIKMLRPVTQLYVSVDAATKDSLKAIDRPLFSDFWERFVDSLKALRD 479

Query: 715  KEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHS 536
            KEQRTVYRLTLVKGWNAEDIDAYA+L  IG PDLVE+KGVTYCGS+ATSKLTMENVPWHS
Sbjct: 480  KEQRTVYRLTLVKGWNAEDIDAYANLLGIGKPDLVEMKGVTYCGSSATSKLTMENVPWHS 539

Query: 535  DVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGK 356
            DVR FSEALAIKS+G+YEVACEHVHSCCV+LAKV K+K+ GQW+TWIDYERFH LVASGK
Sbjct: 540  DVRAFSEALAIKSSGEYEVACEHVHSCCVVLAKVSKFKVKGQWYTWIDYERFHELVASGK 599

Query: 355  PFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIRAS 221
            PFKSE YMA+TPSWAVYGAEEGGFDPDQSR+KKERRHGAA+IR S
Sbjct: 600  PFKSEHYMALTPSWAVYGAEEGGFDPDQSRYKKERRHGAASIRGS 644


>ref|XP_008783491.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like isoform X2 [Phoenix dactylifera]
          Length = 648

 Score =  738 bits (1904), Expect = 0.0
 Identities = 350/405 (86%), Positives = 374/405 (92%)
 Frame = -1

Query: 1435 SRKLVASLVNGENGVKEKSFVNGENGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTK 1256
            SRK   +L NG          NG+NG REMVTP+I  SL+KQGYKI+GSHSGVK+CRWTK
Sbjct: 248  SRKSTGALSNGGQ--------NGDNGLREMVTPIITTSLEKQGYKIVGSHSGVKLCRWTK 299

Query: 1255 SQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVI 1076
            SQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVI
Sbjct: 300  SQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVI 359

Query: 1075 VNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRH 896
            VNAAIDQHTKMIKQMKGVPGV QE+L EGLSP+HCALSLVGEPIMYPEIN+LIDELH RH
Sbjct: 360  VNAAIDQHTKMIKQMKGVPGVKQEQLLEGLSPKHCALSLVGEPIMYPEINSLIDELHSRH 419

Query: 895  ISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRD 716
            ISTFLVTNAQFPD+IK L+P+TQLYVSVDAATKDSLKAIDRPLF DFWERFVDSLKALRD
Sbjct: 420  ISTFLVTNAQFPDRIKMLRPVTQLYVSVDAATKDSLKAIDRPLFSDFWERFVDSLKALRD 479

Query: 715  KEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHS 536
            KEQRTVYRLTLVKGWNAEDIDAYA+L  IG PDLVE+KGVTYCGS+ATSKLTMENVPWHS
Sbjct: 480  KEQRTVYRLTLVKGWNAEDIDAYANLLGIGKPDLVEMKGVTYCGSSATSKLTMENVPWHS 539

Query: 535  DVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGK 356
            DVR FSEALAIKS+G+YEVACEHVHSCCV+LAKV K+K+ GQW+TWIDYERFH LVASGK
Sbjct: 540  DVRAFSEALAIKSSGEYEVACEHVHSCCVVLAKVSKFKVKGQWYTWIDYERFHELVASGK 599

Query: 355  PFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIRAS 221
            PFKSE YMA+TPSWAVYGAEEGGFDPDQSR+KKERRHGAA+IR S
Sbjct: 600  PFKSEHYMALTPSWAVYGAEEGGFDPDQSRYKKERRHGAASIRGS 644


>ref|XP_010942945.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Elaeis guineensis]
          Length = 647

 Score =  734 bits (1895), Expect = 0.0
 Identities = 350/403 (86%), Positives = 371/403 (92%)
 Frame = -1

Query: 1435 SRKLVASLVNGENGVKEKSFVNGENGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTK 1256
            SRK   +L NG          NG+NG REMVTP+IR SL+KQGYKIIGSHSGVK+CRWTK
Sbjct: 252  SRKSTGALSNGGQ--------NGDNGLREMVTPIIRTSLEKQGYKIIGSHSGVKLCRWTK 303

Query: 1255 SQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVI 1076
            SQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVI
Sbjct: 304  SQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVI 363

Query: 1075 VNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRH 896
            VNAAIDQHTKMIKQMKGVPGV QE+L EGLSPRHCALSLVGEPIMYPEIN+LIDELH RH
Sbjct: 364  VNAAIDQHTKMIKQMKGVPGVKQEQLLEGLSPRHCALSLVGEPIMYPEINSLIDELHSRH 423

Query: 895  ISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRD 716
            ISTFLVTNAQFPD+IK L+ +TQLYVSVDAATKDSLKAIDRPLF DFWERFVDSLKAL+D
Sbjct: 424  ISTFLVTNAQFPDRIKMLRSVTQLYVSVDAATKDSLKAIDRPLFSDFWERFVDSLKALQD 483

Query: 715  KEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHS 536
            KEQRTVYRLTLVKGWNAEDIDAYA+L  IG PDLVEIKGVTYCGS+ATSKLTMENVPWHS
Sbjct: 484  KEQRTVYRLTLVKGWNAEDIDAYANLLGIGKPDLVEIKGVTYCGSSATSKLTMENVPWHS 543

Query: 535  DVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGK 356
            DV+ FSEALA+KS+G+YEVACEHVHSCCVLLAK  K+K+ GQW TWIDYERFH LVASGK
Sbjct: 544  DVKAFSEALAVKSSGEYEVACEHVHSCCVLLAKASKFKVKGQWFTWIDYERFHELVASGK 603

Query: 355  PFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 227
            PFKSEDYMA+TPSWAVYGAEEGGFDPDQSR KKERRHGAA+IR
Sbjct: 604  PFKSEDYMALTPSWAVYGAEEGGFDPDQSRCKKERRHGAASIR 646


>ref|NP_001347253.1| uncharacterized protein LOC111828519 [Oryza brachyantha]
          Length = 406

 Score =  721 bits (1861), Expect = 0.0
 Identities = 335/391 (85%), Positives = 364/391 (93%), Gaps = 1/391 (0%)
 Frame = -1

Query: 1402 ENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTKSQLRGRGGCY 1226
            +NG  E +  NG ENG R+MVTP+IRASL+KQGYKIIGSHSGVKICRWTKSQLRGRGGCY
Sbjct: 13   QNGKVEGALSNGGENGPRDMVTPIIRASLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCY 72

Query: 1225 KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIVNAAIDQHTK 1046
            KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMDDPL IVN AIDQHTK
Sbjct: 73   KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDDPLDIVNTAIDQHTK 132

Query: 1045 MIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRHISTFLVTNAQ 866
            M+KQMKGVPGV  ERL+EGLSPRHCALSLVGEPIMYPEIN L+ ELHRRHISTFLVTNAQ
Sbjct: 133  MVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINVLVGELHRRHISTFLVTNAQ 192

Query: 865  FPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRDKEQRTVYRLT 686
            FPDKIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSLK+L +K+QRTVYRLT
Sbjct: 193  FPDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHEKDQRTVYRLT 252

Query: 685  LVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHSDVRDFSEALA 506
            LVKGWN E+IDAYA L ++G PD +EIKGVTYCGS+ TSKLTMENVPWHSDV++FSEALA
Sbjct: 253  LVKGWNVEEIDAYAKLLNLGQPDFIEIKGVTYCGSSTTSKLTMENVPWHSDVKEFSEALA 312

Query: 505  IKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGKPFKSEDYMAV 326
            +KS G YEVACEH HSCCVLLAKVDK+KING+WHTWIDY+RFH LV SGKPFKS+DYMA+
Sbjct: 313  LKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHDLVTSGKPFKSQDYMAL 372

Query: 325  TPSWAVYGAEEGGFDPDQSRFKKERRHGAAA 233
            TPSWAVYGAEEGGFDPDQSR+KKERRHGAAA
Sbjct: 373  TPSWAVYGAEEGGFDPDQSRYKKERRHGAAA 403


>dbj|BAF13343.1| Os03g0775300 [Oryza sativa Japonica Group]
 dbj|BAS86627.1| Os03g0775300 [Oryza sativa Japonica Group]
          Length = 600

 Score =  729 bits (1881), Expect = 0.0
 Identities = 339/392 (86%), Positives = 366/392 (93%), Gaps = 1/392 (0%)
 Frame = -1

Query: 1399 NGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTKSQLRGRGGCYK 1223
            NG  E +  NG ENG R+MVTP+IR SL+KQGYKIIGSHSGVKICRWTKSQLRGRGGCYK
Sbjct: 208  NGKVEGALSNGGENGVRDMVTPIIRTSLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYK 267

Query: 1222 HSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIVNAAIDQHTKM 1043
            HSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMDDPL IVNAAIDQHTKM
Sbjct: 268  HSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDDPLDIVNAAIDQHTKM 327

Query: 1042 IKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRHISTFLVTNAQF 863
            +KQMKGVPGV  ERL+EGLSPRHCALSLVGEPIMYPEIN LIDELHRRHISTFLVTNAQF
Sbjct: 328  VKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINVLIDELHRRHISTFLVTNAQF 387

Query: 862  PDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRDKEQRTVYRLTL 683
            PDKIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSLK+L DK+QRTVYRLTL
Sbjct: 388  PDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHDKDQRTVYRLTL 447

Query: 682  VKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHSDVRDFSEALAI 503
            VKGWNAE+ID YA L  +G PD +EIKGVTYCGS+ATSKLTMENVPWHSDV+DFSEALA+
Sbjct: 448  VKGWNAEEIDGYAKLLSLGQPDFIEIKGVTYCGSSATSKLTMENVPWHSDVKDFSEALAL 507

Query: 502  KSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGKPFKSEDYMAVT 323
            KS G YEVACEH HSCCVLLAKVDK+KING+WHTWIDY+RFH LV SGKPF+S+DYMA+T
Sbjct: 508  KSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHELVTSGKPFRSQDYMALT 567

Query: 322  PSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 227
            PSWAVYGAEEGGFDPDQSR+KKERRHGAAA++
Sbjct: 568  PSWAVYGAEEGGFDPDQSRYKKERRHGAAALK 599


>ref|XP_015631211.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Oryza sativa Japonica Group]
 sp|Q8H8N3.1|TYW1_ORYSJ RecName: Full=S-adenosyl-L-methionine-dependent tRNA
            4-demethylwyosine synthase; AltName: Full=tRNA
            wybutosine-synthesizing protein 1 homolog
 gb|AAN65027.1| putative oxidoreductase [Oryza sativa Japonica Group]
 gb|ABF99122.1| radical S-adenosyl methionine and flavodoxin domains 1, isoform 1,
            putative, expressed [Oryza sativa Japonica Group]
 gb|EAY92018.1| hypothetical protein OsI_13711 [Oryza sativa Indica Group]
          Length = 653

 Score =  729 bits (1881), Expect = 0.0
 Identities = 339/392 (86%), Positives = 366/392 (93%), Gaps = 1/392 (0%)
 Frame = -1

Query: 1399 NGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTKSQLRGRGGCYK 1223
            NG  E +  NG ENG R+MVTP+IR SL+KQGYKIIGSHSGVKICRWTKSQLRGRGGCYK
Sbjct: 261  NGKVEGALSNGGENGVRDMVTPIIRTSLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYK 320

Query: 1222 HSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIVNAAIDQHTKM 1043
            HSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMDDPL IVNAAIDQHTKM
Sbjct: 321  HSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDDPLDIVNAAIDQHTKM 380

Query: 1042 IKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRHISTFLVTNAQF 863
            +KQMKGVPGV  ERL+EGLSPRHCALSLVGEPIMYPEIN LIDELHRRHISTFLVTNAQF
Sbjct: 381  VKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINVLIDELHRRHISTFLVTNAQF 440

Query: 862  PDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRDKEQRTVYRLTL 683
            PDKIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSLK+L DK+QRTVYRLTL
Sbjct: 441  PDKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHDKDQRTVYRLTL 500

Query: 682  VKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHSDVRDFSEALAI 503
            VKGWNAE+ID YA L  +G PD +EIKGVTYCGS+ATSKLTMENVPWHSDV+DFSEALA+
Sbjct: 501  VKGWNAEEIDGYAKLLSLGQPDFIEIKGVTYCGSSATSKLTMENVPWHSDVKDFSEALAL 560

Query: 502  KSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGKPFKSEDYMAVT 323
            KS G YEVACEH HSCCVLLAKVDK+KING+WHTWIDY+RFH LV SGKPF+S+DYMA+T
Sbjct: 561  KSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHELVTSGKPFRSQDYMALT 620

Query: 322  PSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 227
            PSWAVYGAEEGGFDPDQSR+KKERRHGAAA++
Sbjct: 621  PSWAVYGAEEGGFDPDQSRYKKERRHGAAALK 652


>ref|XP_020704408.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Dendrobium catenatum]
          Length = 664

 Score =  721 bits (1861), Expect = 0.0
 Identities = 342/403 (84%), Positives = 369/403 (91%)
 Frame = -1

Query: 1435 SRKLVASLVNGENGVKEKSFVNGENGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTK 1256
            SRK+ A LVNG NGV            +EMVTP+IR +L+KQGYKIIGSHSGVKICRWTK
Sbjct: 256  SRKMKAELVNG-NGV-----------IKEMVTPIIRNNLEKQGYKIIGSHSGVKICRWTK 303

Query: 1255 SQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVI 1076
            +QLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGK WRWKMDDPLVI
Sbjct: 304  AQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKEWRWKMDDPLVI 363

Query: 1075 VNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRH 896
            V+AAIDQHTKMIKQMKGVPGV +++  EGLSPRHCALSLVGEPIMYPEIN LI+ELHRRH
Sbjct: 364  VDAAIDQHTKMIKQMKGVPGVKEQQFLEGLSPRHCALSLVGEPIMYPEINRLIEELHRRH 423

Query: 895  ISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRD 716
            ISTFLVTNAQFPD+IK LKPITQLYVSVDAATK+SLKA+DRPLFGDFWERFVDSLKAL D
Sbjct: 424  ISTFLVTNAQFPDRIKMLKPITQLYVSVDAATKESLKAVDRPLFGDFWERFVDSLKALHD 483

Query: 715  KEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHS 536
            K+QRTVYRLTLVKGWNAE++DAYA+L  +G PD +EIKGVTYCGSTATSKLTMENVPWHS
Sbjct: 484  KQQRTVYRLTLVKGWNAEEVDAYANLLGLGEPDFIEIKGVTYCGSTATSKLTMENVPWHS 543

Query: 535  DVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGK 356
            DV++FSE LAIKSNG YEVACEHVHSCCVLLAKVDK+KINGQWHTWIDYERFH LVASGK
Sbjct: 544  DVKEFSETLAIKSNGLYEVACEHVHSCCVLLAKVDKFKINGQWHTWIDYERFHDLVASGK 603

Query: 355  PFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 227
            PF +EDYMAVTPSWAVYGAEEGGFDP Q R+KKERRHG AA++
Sbjct: 604  PFGTEDYMAVTPSWAVYGAEEGGFDPKQFRYKKERRHGPAALK 646


>gb|KQK86877.1| hypothetical protein SETIT_034607mg [Setaria italica]
          Length = 649

 Score =  719 bits (1857), Expect = 0.0
 Identities = 333/393 (84%), Positives = 364/393 (92%), Gaps = 1/393 (0%)
 Frame = -1

Query: 1402 ENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTKSQLRGRGGCY 1226
            +NG  E S  NG +NG +EMVTP+IR SL+KQGYKI+GSHSGVKICRWTKSQLRGRGGCY
Sbjct: 256  QNGKVEGSLANGGQNGVKEMVTPIIRTSLEKQGYKILGSHSGVKICRWTKSQLRGRGGCY 315

Query: 1225 KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIVNAAIDQHTK 1046
            KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMD+PL I NAAIDQHTK
Sbjct: 316  KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDNPLDIANAAIDQHTK 375

Query: 1045 MIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRHISTFLVTNAQ 866
            MIKQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYPEIN L+DELHRRHISTFLVTNAQ
Sbjct: 376  MIKQMKGVPGVKPEKLEEGLSPRHCALSLVGEPIMYPEINALVDELHRRHISTFLVTNAQ 435

Query: 865  FPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRDKEQRTVYRLT 686
            FP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSLK+L +K+QRTVYRLT
Sbjct: 436  FPEKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHEKDQRTVYRLT 495

Query: 685  LVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHSDVRDFSEALA 506
            LVKGWNAE+IDAYA L ++G PD +EIKGVTYCGS+ATSKLTMENVPWHSDV++FSE LA
Sbjct: 496  LVKGWNAEEIDAYAKLLNLGQPDFIEIKGVTYCGSSATSKLTMENVPWHSDVKEFSEVLA 555

Query: 505  IKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGKPFKSEDYMAV 326
             KS G YEVACEH HSCCVLLAKVDK+KING+WHTWIDY+RFH LV SGKPFKS+DYMA+
Sbjct: 556  SKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHELVTSGKPFKSQDYMAL 615

Query: 325  TPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 227
            TPSWAVYGAEEGGFDPDQSRFKKERRHG AA++
Sbjct: 616  TPSWAVYGAEEGGFDPDQSRFKKERRHGTAALK 648


>ref|NP_001347248.1| uncharacterized protein LOC100273754 [Zea mays]
 gb|ONM09874.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Zea mays]
          Length = 642

 Score =  719 bits (1856), Expect = 0.0
 Identities = 334/394 (84%), Positives = 362/394 (91%), Gaps = 1/394 (0%)
 Frame = -1

Query: 1402 ENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTKSQLRGRGGCY 1226
            +NG  E    NG  NG ++MVTP+IR SL+KQGYKIIGSHSGVKICRWTKSQLRGRGGCY
Sbjct: 249  KNGNLEGVLANGVHNGVKDMVTPIIRTSLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCY 308

Query: 1225 KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIVNAAIDQHTK 1046
            KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMDDPL IVNAAIDQHTK
Sbjct: 309  KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDDPLDIVNAAIDQHTK 368

Query: 1045 MIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRHISTFLVTNAQ 866
            M+KQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYPEIN L+DELHRRHISTFLVTNAQ
Sbjct: 369  MVKQMKGVPGVKPEKLDEGLSPRHCALSLVGEPIMYPEINALVDELHRRHISTFLVTNAQ 428

Query: 865  FPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRDKEQRTVYRLT 686
            FP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSLK+L +K+QRTVYRLT
Sbjct: 429  FPEKIKALKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHEKDQRTVYRLT 488

Query: 685  LVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHSDVRDFSEALA 506
            LVKGWNAE+IDAYA L D+G PD +EIKGVTYCGS+ATSKLTMENVPWHSDV++FSE LA
Sbjct: 489  LVKGWNAEEIDAYAKLLDLGQPDFIEIKGVTYCGSSATSKLTMENVPWHSDVKEFSEVLA 548

Query: 505  IKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGKPFKSEDYMAV 326
             KS G YEVACEH HSCCVLLAKVDK+KING+WHTWIDY+RFH LV SGKPF S DYMA+
Sbjct: 549  SKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHELVTSGKPFNSRDYMAM 608

Query: 325  TPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIRA 224
            TPSWAVYGAEEGGFDPDQSRFKKERRHG AA+R+
Sbjct: 609  TPSWAVYGAEEGGFDPDQSRFKKERRHGTAALRS 642


>ref|XP_009399072.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Musa acuminata subsp. malaccensis]
          Length = 647

 Score =  719 bits (1855), Expect = 0.0
 Identities = 335/386 (86%), Positives = 367/386 (95%), Gaps = 1/386 (0%)
 Frame = -1

Query: 1381 SFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGI 1205
            +  NG ENG ++MVTPVIR +L+KQGYKIIGSHSGVK+CRWTKSQLRGRGGCYKHSFYGI
Sbjct: 261  ALTNGWENGVKDMVTPVIRVNLEKQGYKIIGSHSGVKLCRWTKSQLRGRGGCYKHSFYGI 320

Query: 1204 ESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIVNAAIDQHTKMIKQMKG 1025
            ESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMD+PL IV+ AID+HTKMIKQMKG
Sbjct: 321  ESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDNPLEIVDVAIDEHTKMIKQMKG 380

Query: 1024 VPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRHISTFLVTNAQFPDKIKE 845
            VPGV  ERLSEGLSPRHCALSLVGEPIMYPEINTL+DELHRRHISTFLVTNAQFPD+IK 
Sbjct: 381  VPGVKPERLSEGLSPRHCALSLVGEPIMYPEINTLVDELHRRHISTFLVTNAQFPDRIKM 440

Query: 844  LKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRDKEQRTVYRLTLVKGWNA 665
            LKPITQLYVSVDAATKDSLKAIDRPLFGDFWERF+DSLKAL+DKEQRTVYRLTLVKGWNA
Sbjct: 441  LKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFLDSLKALQDKEQRTVYRLTLVKGWNA 500

Query: 664  EDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHSDVRDFSEALAIKSNGQY 485
            ED++AYA+L  IG PDLVEIKGVTYCG++ATSKLTM+NVPWHSDV+ FSEALA+KS+G+Y
Sbjct: 501  EDVEAYANLLGIGKPDLVEIKGVTYCGTSATSKLTMDNVPWHSDVKAFSEALAMKSSGEY 560

Query: 484  EVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGKPFKSEDYMAVTPSWAVY 305
            EVACEH HSCCVLLAKVDK+KI G+W+TWIDYERFH LVASGK FK+EDYMA TPSWAVY
Sbjct: 561  EVACEHAHSCCVLLAKVDKFKIKGEWYTWIDYERFHELVASGKSFKTEDYMARTPSWAVY 620

Query: 304  GAEEGGFDPDQSRFKKERRHGAAAIR 227
            GAEEGGFDPDQSR++KERRHGAA+++
Sbjct: 621  GAEEGGFDPDQSRYRKERRHGAASVQ 646


>ref|NP_001347259.1| uncharacterized LOC111828523 [Sorghum bicolor]
          Length = 652

 Score =  718 bits (1854), Expect = 0.0
 Identities = 334/391 (85%), Positives = 361/391 (92%), Gaps = 1/391 (0%)
 Frame = -1

Query: 1402 ENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTKSQLRGRGGCY 1226
            +NG  E    NG +NG ++MVTP+IR SL+KQGYKIIGSHSGVKICRWTKSQLRGRGGCY
Sbjct: 257  KNGNLEGVVANGVQNGVKDMVTPIIRTSLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCY 316

Query: 1225 KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIVNAAIDQHTK 1046
            KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMDDPL IVNAAIDQHTK
Sbjct: 317  KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDDPLDIVNAAIDQHTK 376

Query: 1045 MIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRHISTFLVTNAQ 866
            M+KQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYPEIN L+DELHRRHISTFLVTNAQ
Sbjct: 377  MVKQMKGVPGVKPEKLEEGLSPRHCALSLVGEPIMYPEINALVDELHRRHISTFLVTNAQ 436

Query: 865  FPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRDKEQRTVYRLT 686
            FP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSLK+L +K+QRTVYRLT
Sbjct: 437  FPEKIKALKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHEKDQRTVYRLT 496

Query: 685  LVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHSDVRDFSEALA 506
            LVKGWNAE+IDAYA L D+G PD +EIKGVTYCGS+ATSKLTMENVPWHSDV++FSE LA
Sbjct: 497  LVKGWNAEEIDAYAKLLDLGQPDFIEIKGVTYCGSSATSKLTMENVPWHSDVKEFSEVLA 556

Query: 505  IKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGKPFKSEDYMAV 326
             KS G YEVACEH HSCCVLLAKVDK+KING+WHTWIDY+RFH LV SGKPFKS DYMA+
Sbjct: 557  SKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHELVTSGKPFKSRDYMAM 616

Query: 325  TPSWAVYGAEEGGFDPDQSRFKKERRHGAAA 233
            TPSWAVYGAEEGGFDPDQSRFKKERRHG AA
Sbjct: 617  TPSWAVYGAEEGGFDPDQSRFKKERRHGTAA 647


>ref|NP_001347566.1| uncharacterized protein LOC111946219 [Setaria italica]
          Length = 692

 Score =  719 bits (1857), Expect = 0.0
 Identities = 333/393 (84%), Positives = 364/393 (92%), Gaps = 1/393 (0%)
 Frame = -1

Query: 1402 ENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTKSQLRGRGGCY 1226
            +NG  E S  NG +NG +EMVTP+IR SL+KQGYKI+GSHSGVKICRWTKSQLRGRGGCY
Sbjct: 299  QNGKVEGSLANGGQNGVKEMVTPIIRTSLEKQGYKILGSHSGVKICRWTKSQLRGRGGCY 358

Query: 1225 KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIVNAAIDQHTK 1046
            KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMD+PL I NAAIDQHTK
Sbjct: 359  KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDNPLDIANAAIDQHTK 418

Query: 1045 MIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRHISTFLVTNAQ 866
            MIKQMKGVPGV  E+L EGLSPRHCALSLVGEPIMYPEIN L+DELHRRHISTFLVTNAQ
Sbjct: 419  MIKQMKGVPGVKPEKLEEGLSPRHCALSLVGEPIMYPEINALVDELHRRHISTFLVTNAQ 478

Query: 865  FPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRDKEQRTVYRLT 686
            FP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSLK+L +K+QRTVYRLT
Sbjct: 479  FPEKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHEKDQRTVYRLT 538

Query: 685  LVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHSDVRDFSEALA 506
            LVKGWNAE+IDAYA L ++G PD +EIKGVTYCGS+ATSKLTMENVPWHSDV++FSE LA
Sbjct: 539  LVKGWNAEEIDAYAKLLNLGQPDFIEIKGVTYCGSSATSKLTMENVPWHSDVKEFSEVLA 598

Query: 505  IKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGKPFKSEDYMAV 326
             KS G YEVACEH HSCCVLLAKVDK+KING+WHTWIDY+RFH LV SGKPFKS+DYMA+
Sbjct: 599  SKSGGVYEVACEHAHSCCVLLAKVDKFKINGKWHTWIDYDRFHELVTSGKPFKSQDYMAL 658

Query: 325  TPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 227
            TPSWAVYGAEEGGFDPDQSRFKKERRHG AA++
Sbjct: 659  TPSWAVYGAEEGGFDPDQSRFKKERRHGTAALK 691


>ref|XP_020180383.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Aegilops tauschii subsp. tauschii]
          Length = 659

 Score =  717 bits (1850), Expect = 0.0
 Identities = 331/393 (84%), Positives = 366/393 (93%), Gaps = 1/393 (0%)
 Frame = -1

Query: 1402 ENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTKSQLRGRGGCY 1226
            +NG  E +  NG +NGAR MVTP+IRASL+KQGYKI+GSHSGVKICRWTKSQLRGRGGCY
Sbjct: 266  QNGKVESALPNGGQNGARAMVTPIIRASLEKQGYKIVGSHSGVKICRWTKSQLRGRGGCY 325

Query: 1225 KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIVNAAIDQHTK 1046
            KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMDDPL IVN AID+H K
Sbjct: 326  KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDDPLDIVNTAIDEHRK 385

Query: 1045 MIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRHISTFLVTNAQ 866
            M+KQMKGVPGV  ERL+EGLSPRHCALSLVGEPIMYPEINTL+D+LHRRHISTFLVTNAQ
Sbjct: 386  MVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINTLVDDLHRRHISTFLVTNAQ 445

Query: 865  FPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRDKEQRTVYRLT 686
            FP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSLK+L DK+QRTVYRLT
Sbjct: 446  FPEKIKALKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHDKDQRTVYRLT 505

Query: 685  LVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHSDVRDFSEALA 506
            LVKGWN E+IDAYA+L  +G PD +EIKGVTYCGS+ATSKLTMENVPWH+DV++FSE LA
Sbjct: 506  LVKGWNVEEIDAYANLLKLGQPDFIEIKGVTYCGSSATSKLTMENVPWHADVKEFSEVLA 565

Query: 505  IKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGKPFKSEDYMAV 326
             KS G YE+ACEHVHSCCVLLAKVDK+KING+WHTWIDY+RF+ LV SGKPFKS DYMAV
Sbjct: 566  SKSGGVYELACEHVHSCCVLLAKVDKFKINGKWHTWIDYDRFNELVTSGKPFKSSDYMAV 625

Query: 325  TPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 227
            TPSWAVYGA+EGGFDPDQ+RFKKERRHGAAA++
Sbjct: 626  TPSWAVYGADEGGFDPDQARFKKERRHGAAALK 658


>dbj|BAK06688.1| predicted protein [Hordeum vulgare subsp. vulgare]
          Length = 664

 Score =  716 bits (1847), Expect = 0.0
 Identities = 330/393 (83%), Positives = 366/393 (93%), Gaps = 1/393 (0%)
 Frame = -1

Query: 1402 ENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTKSQLRGRGGCY 1226
            +NG  E +  NG +NGAR MVTP+IRASL+KQGYKI+GSHSGVKICRWTKSQLRGRGGCY
Sbjct: 271  QNGKVEGALPNGGQNGARAMVTPIIRASLEKQGYKIVGSHSGVKICRWTKSQLRGRGGCY 330

Query: 1225 KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIVNAAIDQHTK 1046
            KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMDDPL IVN AID+H K
Sbjct: 331  KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDDPLDIVNTAIDEHRK 390

Query: 1045 MIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRHISTFLVTNAQ 866
            M+KQMKGVPGV  ERL+EGLSPRHCALSLVGEPIMYPEINTL+D+LHRRHISTFLVTNAQ
Sbjct: 391  MVKQMKGVPGVKPERLAEGLSPRHCALSLVGEPIMYPEINTLVDDLHRRHISTFLVTNAQ 450

Query: 865  FPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRDKEQRTVYRLT 686
            FP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSLK+L DK+QRTVYRLT
Sbjct: 451  FPEKIKALKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHDKDQRTVYRLT 510

Query: 685  LVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHSDVRDFSEALA 506
            LVKGWN E+IDAYA+L  +G PD +E+KGVTYCGS+ATSKLTMENVPWH+DV++FSE LA
Sbjct: 511  LVKGWNVEEIDAYANLLKLGQPDFIEVKGVTYCGSSATSKLTMENVPWHADVKEFSEVLA 570

Query: 505  IKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGKPFKSEDYMAV 326
             KS G YE+ACEHVHSCCVLLAKVDK+KING+WHTWIDY+RF+ LV SGKPFKS DYMAV
Sbjct: 571  TKSGGVYELACEHVHSCCVLLAKVDKFKINGKWHTWIDYDRFNELVMSGKPFKSSDYMAV 630

Query: 325  TPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 227
            TPSWAVYGA+EGGFDPDQ+RFKKERRHGAAA++
Sbjct: 631  TPSWAVYGADEGGFDPDQARFKKERRHGAAALK 663


>ref|XP_020092095.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Ananas comosus]
          Length = 655

 Score =  715 bits (1846), Expect = 0.0
 Identities = 340/400 (85%), Positives = 371/400 (92%), Gaps = 1/400 (0%)
 Frame = -1

Query: 1435 SRKLVASLVNGENGVKEKSFVNGE-NGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWT 1259
            SRK   +LVNG++     + VNG+ +G REMVTP+IR SL+KQGYKIIGSHSGVK+CRWT
Sbjct: 257  SRKSSVTLVNGKSS---GTLVNGKGDGLREMVTPIIRTSLEKQGYKIIGSHSGVKLCRWT 313

Query: 1258 KSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLV 1079
            KSQLRGRGGCYKH+FYGIESHRCMEATPSLACANKCVFCWRHH NPVGKSWRWKMDDPL 
Sbjct: 314  KSQLRGRGGCYKHAFYGIESHRCMEATPSLACANKCVFCWRHHANPVGKSWRWKMDDPLE 373

Query: 1078 IVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRR 899
            IV+AAIDQHTKMIKQMKGVPGV QERL EGLSPRHCALSLVGEPIMYPEINTLIDELH+R
Sbjct: 374  IVDAAIDQHTKMIKQMKGVPGVKQERLDEGLSPRHCALSLVGEPIMYPEINTLIDELHQR 433

Query: 898  HISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALR 719
             ISTFLVTNAQFPD+IK LKPITQLYVSVDA+TK+SLKAIDRPLFGDFWERF+DSLKAL 
Sbjct: 434  RISTFLVTNAQFPDRIKMLKPITQLYVSVDASTKESLKAIDRPLFGDFWERFLDSLKALH 493

Query: 718  DKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWH 539
            DKEQRTVYRLTLVKGWNAE+IDAYA+L  +G PDLVEIKGVTYCGS+ATSKLTMENVPWH
Sbjct: 494  DKEQRTVYRLTLVKGWNAEEIDAYANLLTMGKPDLVEIKGVTYCGSSATSKLTMENVPWH 553

Query: 538  SDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASG 359
            SDV+ FSEALA+KSNG YEVACEHVHSCCVLLAKVDK+K++G+W+TWIDYERF  LVASG
Sbjct: 554  SDVKAFSEALAVKSNGIYEVACEHVHSCCVLLAKVDKFKVDGKWYTWIDYERFQELVASG 613

Query: 358  KPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGA 239
             PF++EDY A+TPSWAVYGAEEGGFDP QSR+KKERRHGA
Sbjct: 614  TPFRTEDYTALTPSWAVYGAEEGGFDPGQSRYKKERRHGA 653


>gb|OAY80972.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Ananas comosus]
          Length = 663

 Score =  715 bits (1846), Expect = 0.0
 Identities = 340/400 (85%), Positives = 371/400 (92%), Gaps = 1/400 (0%)
 Frame = -1

Query: 1435 SRKLVASLVNGENGVKEKSFVNGE-NGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWT 1259
            SRK   +LVNG++     + VNG+ +G REMVTP+IR SL+KQGYKIIGSHSGVK+CRWT
Sbjct: 265  SRKSSVTLVNGKSS---GTLVNGKGDGLREMVTPIIRTSLEKQGYKIIGSHSGVKLCRWT 321

Query: 1258 KSQLRGRGGCYKHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLV 1079
            KSQLRGRGGCYKH+FYGIESHRCMEATPSLACANKCVFCWRHH NPVGKSWRWKMDDPL 
Sbjct: 322  KSQLRGRGGCYKHAFYGIESHRCMEATPSLACANKCVFCWRHHANPVGKSWRWKMDDPLE 381

Query: 1078 IVNAAIDQHTKMIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRR 899
            IV+AAIDQHTKMIKQMKGVPGV QERL EGLSPRHCALSLVGEPIMYPEINTLIDELH+R
Sbjct: 382  IVDAAIDQHTKMIKQMKGVPGVKQERLDEGLSPRHCALSLVGEPIMYPEINTLIDELHQR 441

Query: 898  HISTFLVTNAQFPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALR 719
             ISTFLVTNAQFPD+IK LKPITQLYVSVDA+TK+SLKAIDRPLFGDFWERF+DSLKAL 
Sbjct: 442  RISTFLVTNAQFPDRIKMLKPITQLYVSVDASTKESLKAIDRPLFGDFWERFLDSLKALH 501

Query: 718  DKEQRTVYRLTLVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWH 539
            DKEQRTVYRLTLVKGWNAE+IDAYA+L  +G PDLVEIKGVTYCGS+ATSKLTMENVPWH
Sbjct: 502  DKEQRTVYRLTLVKGWNAEEIDAYANLLTMGKPDLVEIKGVTYCGSSATSKLTMENVPWH 561

Query: 538  SDVRDFSEALAIKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASG 359
            SDV+ FSEALA+KSNG YEVACEHVHSCCVLLAKVDK+K++G+W+TWIDYERF  LVASG
Sbjct: 562  SDVKAFSEALAVKSNGIYEVACEHVHSCCVLLAKVDKFKVDGKWYTWIDYERFQELVASG 621

Query: 358  KPFKSEDYMAVTPSWAVYGAEEGGFDPDQSRFKKERRHGA 239
             PF++EDY A+TPSWAVYGAEEGGFDP QSR+KKERRHGA
Sbjct: 622  TPFRTEDYTALTPSWAVYGAEEGGFDPGQSRYKKERRHGA 661


>ref|XP_014752193.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Brachypodium distachyon]
          Length = 653

 Score =  713 bits (1841), Expect = 0.0
 Identities = 332/393 (84%), Positives = 365/393 (92%), Gaps = 1/393 (0%)
 Frame = -1

Query: 1402 ENGVKEKSFVNG-ENGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTKSQLRGRGGCY 1226
            +NG K     NG ENGAREMVTP+IR SL+KQGYKI+GSHSGVKICRWTKSQLRGRGGCY
Sbjct: 261  QNG-KVAGLPNGAENGAREMVTPIIRTSLEKQGYKIVGSHSGVKICRWTKSQLRGRGGCY 319

Query: 1225 KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIVNAAIDQHTK 1046
            KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMDDPL IVN AID+H K
Sbjct: 320  KHSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWKWKMDDPLDIVNTAIDEHRK 379

Query: 1045 MIKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRHISTFLVTNAQ 866
            M+KQMKGVPGV  E L+EGLSPRHCALSLVGEPIMYPEINTL+D+LHRRHISTFLVTNAQ
Sbjct: 380  MVKQMKGVPGVKPELLAEGLSPRHCALSLVGEPIMYPEINTLVDDLHRRHISTFLVTNAQ 439

Query: 865  FPDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRDKEQRTVYRLT 686
            FP+KIK LKPITQLYVSVDAATK+SLKA+DRPLF DFWERF+DSLK+L DK+QRTVYRLT
Sbjct: 440  FPEKIKTLKPITQLYVSVDAATKESLKAVDRPLFSDFWERFLDSLKSLHDKDQRTVYRLT 499

Query: 685  LVKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHSDVRDFSEALA 506
            LVKGWNAE+IDAYA+L ++G PD +EIKGVTYCGS+ATSKLTMENVPWH+DV++FSE LA
Sbjct: 500  LVKGWNAEEIDAYANLLNLGQPDFIEIKGVTYCGSSATSKLTMENVPWHADVKEFSEVLA 559

Query: 505  IKSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGKPFKSEDYMAV 326
             KS G YE+ACEHVHSCCVLLAKVDK+KING+WHTWIDY+RFH LV SGKPFKS DYMA+
Sbjct: 560  SKSGGVYELACEHVHSCCVLLAKVDKFKINGKWHTWIDYDRFHELVTSGKPFKSCDYMAL 619

Query: 325  TPSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 227
            TPSWAVYGAEEGGFDPDQSR+KKERRHG AA++
Sbjct: 620  TPSWAVYGAEEGGFDPDQSRYKKERRHGTAALK 652


>gb|KZV28868.1| S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Dorcoceras hygrometricum]
          Length = 658

 Score =  713 bits (1840), Expect = 0.0
 Identities = 337/392 (85%), Positives = 360/392 (91%), Gaps = 2/392 (0%)
 Frame = -1

Query: 1396 GVKEKSFVNGE--NGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTKSQLRGRGGCYK 1223
            G      VNGE  NG +EMVTPVIRASLQKQGYKIIGSHSGVK+CRWTKSQLRGRGGCYK
Sbjct: 264  GTTANGKVNGEVVNGEKEMVTPVIRASLQKQGYKIIGSHSGVKLCRWTKSQLRGRGGCYK 323

Query: 1222 HSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIVNAAIDQHTKM 1043
            HSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIV+ AID HTKM
Sbjct: 324  HSFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIVDTAIDLHTKM 383

Query: 1042 IKQMKGVPGVTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRHISTFLVTNAQF 863
            I+QMKGVPGV  ERLSEGLSPRHCALSLVGEPIMYPEIN LIDELHRR ISTFLVTNAQF
Sbjct: 384  IRQMKGVPGVKAERLSEGLSPRHCALSLVGEPIMYPEINALIDELHRRRISTFLVTNAQF 443

Query: 862  PDKIKELKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRDKEQRTVYRLTL 683
            P++IK LKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFV+SLKALR+K+QRTVYRLTL
Sbjct: 444  PERIKTLKPITQLYVSVDAATKDSLKAIDRPLFGDFWERFVESLKALREKQQRTVYRLTL 503

Query: 682  VKGWNAEDIDAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHSDVRDFSEALAI 503
            VKGWN EDIDAY+SLFD+GNPD +EIKGVTYCGS+ATSKLTMENVPWHSDV++FSEALA 
Sbjct: 504  VKGWNTEDIDAYSSLFDLGNPDFIEIKGVTYCGSSATSKLTMENVPWHSDVKNFSEALAQ 563

Query: 502  KSNGQYEVACEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGKPFKSEDYMAVT 323
            KSNG YEVACEH HSCCVLL KV K+K+ G+W TWIDY++FH LVASGKPF SEDYM+ T
Sbjct: 564  KSNGAYEVACEHEHSCCVLLGKVSKFKVEGEWFTWIDYDKFHDLVASGKPFTSEDYMSPT 623

Query: 322  PSWAVYGAEEGGFDPDQSRFKKERRHGAAAIR 227
            P WAVYGAEEGGFDPDQSRFKKERRH  +A +
Sbjct: 624  PRWAVYGAEEGGFDPDQSRFKKERRHHKSAAK 655


>ref|XP_017981378.1| PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Theobroma cacao]
          Length = 637

 Score =  710 bits (1832), Expect = 0.0
 Identities = 332/377 (88%), Positives = 355/377 (94%), Gaps = 1/377 (0%)
 Frame = -1

Query: 1372 NGE-NGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESH 1196
            NG+ +G REMVTPVIRA+L+KQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESH
Sbjct: 257  NGKMDGKREMVTPVIRANLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESH 316

Query: 1195 RCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIVNAAIDQHTKMIKQMKGVPG 1016
            RCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMDDPL IVN AID HTKMIKQMKGVPG
Sbjct: 317  RCMEATPSLACANKCVFCWRHHTNPVGKSWQWKMDDPLEIVNTAIDLHTKMIKQMKGVPG 376

Query: 1015 VTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRHISTFLVTNAQFPDKIKELKP 836
            VTQERL+EGL+PRHCALSLVGEPIMYPEIN L+DELHRR ISTFLVTNAQFPDKIK LKP
Sbjct: 377  VTQERLTEGLTPRHCALSLVGEPIMYPEINMLVDELHRRRISTFLVTNAQFPDKIKMLKP 436

Query: 835  ITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRDKEQRTVYRLTLVKGWNAEDI 656
            ITQLYVSVDAATKDSLKAIDRPLFGDFWERF+DSLKALRDK+QRTVYRLTLVKGWN ED 
Sbjct: 437  ITQLYVSVDAATKDSLKAIDRPLFGDFWERFIDSLKALRDKQQRTVYRLTLVKGWNTEDA 496

Query: 655  DAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHSDVRDFSEALAIKSNGQYEVA 476
            DAY+ LF IG PD VEIKGVTYCGS+ATSKLTMENVPWHSDV+ FSEALA+KS G+YEVA
Sbjct: 497  DAYSKLFSIGKPDFVEIKGVTYCGSSATSKLTMENVPWHSDVKAFSEALALKSEGEYEVA 556

Query: 475  CEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGKPFKSEDYMAVTPSWAVYGAE 296
            CEH HSCCVLLAK DK+K+NGQW+TWIDYE+FH LVASG+PF S DYMA+TPSWAVYGAE
Sbjct: 557  CEHAHSCCVLLAKTDKFKVNGQWYTWIDYEKFHDLVASGEPFDSADYMALTPSWAVYGAE 616

Query: 295  EGGFDPDQSRFKKERRH 245
            EGGFDPDQSR++KER H
Sbjct: 617  EGGFDPDQSRYRKERHH 633


>gb|EOY14757.1| Flavodoxin family protein / radical SAM domain-containing protein
            isoform 1 [Theobroma cacao]
          Length = 637

 Score =  710 bits (1832), Expect = 0.0
 Identities = 332/377 (88%), Positives = 355/377 (94%), Gaps = 1/377 (0%)
 Frame = -1

Query: 1372 NGE-NGAREMVTPVIRASLQKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESH 1196
            NG+ +G REMVTPVIRA+L+KQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESH
Sbjct: 257  NGKMDGKREMVTPVIRANLEKQGYKIIGSHSGVKICRWTKSQLRGRGGCYKHSFYGIESH 316

Query: 1195 RCMEATPSLACANKCVFCWRHHTNPVGKSWRWKMDDPLVIVNAAIDQHTKMIKQMKGVPG 1016
            RCMEATPSLACANKCVFCWRHHTNPVGKSW+WKMDDPL IVN AID HTKMIKQMKGVPG
Sbjct: 317  RCMEATPSLACANKCVFCWRHHTNPVGKSWQWKMDDPLEIVNTAIDLHTKMIKQMKGVPG 376

Query: 1015 VTQERLSEGLSPRHCALSLVGEPIMYPEINTLIDELHRRHISTFLVTNAQFPDKIKELKP 836
            VTQERL+EGL+PRHCALSLVGEPIMYPEIN L+DELHRR ISTFLVTNAQFPDKIK LKP
Sbjct: 377  VTQERLTEGLTPRHCALSLVGEPIMYPEINMLVDELHRRRISTFLVTNAQFPDKIKMLKP 436

Query: 835  ITQLYVSVDAATKDSLKAIDRPLFGDFWERFVDSLKALRDKEQRTVYRLTLVKGWNAEDI 656
            ITQLYVSVDAATKDSLKAIDRPLFGDFWERF+DSLKALRDK+QRTVYRLTLVKGWN ED 
Sbjct: 437  ITQLYVSVDAATKDSLKAIDRPLFGDFWERFIDSLKALRDKQQRTVYRLTLVKGWNTEDA 496

Query: 655  DAYASLFDIGNPDLVEIKGVTYCGSTATSKLTMENVPWHSDVRDFSEALAIKSNGQYEVA 476
            DAY+ LF IG PD VEIKGVTYCGS+ATSKLTMENVPWHSDV+ FSEALA+KS G+YEVA
Sbjct: 497  DAYSKLFSIGKPDFVEIKGVTYCGSSATSKLTMENVPWHSDVKAFSEALALKSEGEYEVA 556

Query: 475  CEHVHSCCVLLAKVDKYKINGQWHTWIDYERFHALVASGKPFKSEDYMAVTPSWAVYGAE 296
            CEH HSCCVLLAK DK+K+NGQW+TWIDYE+FH LVASG+PF S DYMA+TPSWAVYGAE
Sbjct: 557  CEHAHSCCVLLAKTDKFKVNGQWYTWIDYEKFHDLVASGEPFDSADYMALTPSWAVYGAE 616

Query: 295  EGGFDPDQSRFKKERRH 245
            EGGFDPDQSR++KER H
Sbjct: 617  EGGFDPDQSRYRKERHH 633