BLASTX nr result

ID: Ophiopogon22_contig00021436 seq

BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon22_contig00021436
         (415 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ONK67045.1| uncharacterized protein A4U43_C06F15080 [Asparagu...    77   1e-13
ref|XP_020270986.1| putative disease resistance protein At1g6335...    77   1e-13
ref|XP_018472705.1| PREDICTED: probable disease resistance prote...    59   2e-07
gb|PAN50570.1| hypothetical protein PAHAL_H02425 [Panicum hallii]      59   2e-07
ref|XP_020242295.1| disease resistance protein RFL1-like [Aspara...    57   1e-06
ref|XP_004984978.1| disease resistance protein RPS2 [Setaria ita...    57   2e-06
ref|XP_003558328.1| PREDICTED: probable disease resistance prote...    57   2e-06
gb|OEL16537.1| Disease resistance protein RPS2 [Dichanthelium ol...    56   2e-06
dbj|BAF11498.2| Os03g0254000 [Oryza sativa Japonica Group]             56   3e-06
gb|ESQ47302.1| hypothetical protein EUTSA_v10028060mg [Eutrema s...    56   3e-06
ref|XP_009147820.1| PREDICTED: probable disease resistance prote...    56   3e-06
gb|ACP30592.1| disease resistance protein [Brassica rapa subsp. ...    56   3e-06
ref|XP_024014671.1| LOW QUALITY PROTEIN: probable disease resist...    56   3e-06
gb|EEE58713.1| hypothetical protein OsJ_10170 [Oryza sativa Japo...    56   3e-06
ref|XP_018457909.1| PREDICTED: LOW QUALITY PROTEIN: probable dis...    56   3e-06
ref|XP_010654604.1| PREDICTED: probable disease resistance prote...    56   3e-06
gb|EEC74881.1| hypothetical protein OsI_10792 [Oryza sativa Indi...    56   3e-06
ref|XP_015628423.1| PREDICTED: probable disease resistance prote...    56   3e-06
emb|CBI36041.3| unnamed protein product, partial [Vitis vinifera]      56   3e-06
gb|AFE85505.1| putative CC-NBS-LRR disease resistance protein, p...    55   5e-06

>gb|ONK67045.1| uncharacterized protein A4U43_C06F15080 [Asparagus officinalis]
          Length = 887

 Score = 77.0 bits (188), Expect = 1e-13
 Identities = 54/140 (38%), Positives = 72/140 (51%), Gaps = 2/140 (1%)
 Frame = +1

Query: 1   NLTTLILRGN-LWLKSMSILKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTRI 177
           NL TL+L+GN L  K + ++++   LKYLDLS    +  FP  +C LNKLEYLNLS+  I
Sbjct: 514 NLNTLMLKGNRLGFKELEVVRNFHQLKYLDLSRN-GLDHFPPAVCQLNKLEYLNLSKNLI 572

Query: 178 KSLPEEXXXXXXXXXXXXRGTRISTVPAXXXXXXXXXXXXXXFNSGV-AQSSIIDHLGCL 354
            SLP E                IS VP+               ++ V +Q  I+D++  L
Sbjct: 573 ASLPMELKELTMLKYLLLEANLISEVPSELFSSLRSLEVLKLDSARVGSQRLIVDNMDSL 632

Query: 355 EGRLVALGIKASSLLDLRKL 414
           +GRL  L I A S  DL KL
Sbjct: 633 KGRLKGLSINACSQTDLDKL 652


>ref|XP_020270986.1| putative disease resistance protein At1g63350 [Asparagus
           officinalis]
          Length = 914

 Score = 77.0 bits (188), Expect = 1e-13
 Identities = 54/140 (38%), Positives = 72/140 (51%), Gaps = 2/140 (1%)
 Frame = +1

Query: 1   NLTTLILRGN-LWLKSMSILKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTRI 177
           NL TL+L+GN L  K + ++++   LKYLDLS    +  FP  +C LNKLEYLNLS+  I
Sbjct: 541 NLNTLMLKGNRLGFKELEVVRNFHQLKYLDLSRN-GLDHFPPAVCQLNKLEYLNLSKNLI 599

Query: 178 KSLPEEXXXXXXXXXXXXRGTRISTVPAXXXXXXXXXXXXXXFNSGV-AQSSIIDHLGCL 354
            SLP E                IS VP+               ++ V +Q  I+D++  L
Sbjct: 600 ASLPMELKELTMLKYLLLEANLISEVPSELFSSLRSLEVLKLDSARVGSQRLIVDNMDSL 659

Query: 355 EGRLVALGIKASSLLDLRKL 414
           +GRL  L I A S  DL KL
Sbjct: 660 KGRLKGLSINACSQTDLDKL 679


>ref|XP_018472705.1| PREDICTED: probable disease resistance protein At1g15890 [Raphanus
           sativus]
          Length = 809

 Score = 58.9 bits (141), Expect = 2e-07
 Identities = 34/63 (53%), Positives = 42/63 (66%), Gaps = 1/63 (1%)
 Frame = +1

Query: 1   NLTTLILRGN-LWLKSMSILKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTRI 177
           NL+TLILR N L +      + + +L  LDLSD I ++EFP EIC L  L+YLNLS TRI
Sbjct: 497 NLSTLILRDNCLKVIQGKFFQFMRALVVLDLSDNILLREFPEEICSLTSLQYLNLSSTRI 556

Query: 178 KSL 186
            SL
Sbjct: 557 SSL 559


>gb|PAN50570.1| hypothetical protein PAHAL_H02425 [Panicum hallii]
          Length = 915

 Score = 58.9 bits (141), Expect = 2e-07
 Identities = 45/145 (31%), Positives = 63/145 (43%), Gaps = 10/145 (6%)
 Frame = +1

Query: 10  TLILRGNLWL--KSMSILKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTRIKS 183
           +L+L+GN  L  + +  ++H   L YLDL D      FP+EIC L  LEYLNLS+ RI S
Sbjct: 549 SLMLQGNRALPKRMLQAIQHFTKLAYLDLEDTGIQDAFPMEICCLVNLEYLNLSKNRILS 608

Query: 184 LPEEXXXXXXXXXXXXRGTRI--STVPAXXXXXXXXXXXXXXFNSGVAQ------SSIID 339
           LP E            R       T+P               F + +        S +ID
Sbjct: 609 LPVELGNLSQLKYFYLRDNYYIQITIPPGLVSRLGKLQVLEVFTASIVSVADDYVSPVID 668

Query: 340 HLGCLEGRLVALGIKASSLLDLRKL 414
            L     R+ +LGI   +  D+ +L
Sbjct: 669 DLESSGARVASLGIWLDNTRDVERL 693


>ref|XP_020242295.1| disease resistance protein RFL1-like [Asparagus officinalis]
 gb|ONK61383.1| uncharacterized protein A4U43_C08F29340 [Asparagus officinalis]
          Length = 388

 Score = 56.6 bits (135), Expect = 1e-06
 Identities = 50/145 (34%), Positives = 69/145 (47%), Gaps = 7/145 (4%)
 Frame = +1

Query: 1   NLTTLILRG---NLWLKSMSILKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRT 171
           NL TL++      L    +  +++  +L +LDLS  + +   P+EIC L+KLEYLNLS  
Sbjct: 182 NLVTLVIDYCGIGLLSYGLGSIQNFRNLTFLDLSSNV-LGGIPVEICRLSKLEYLNLSAN 240

Query: 172 -RIKSLPEEXXXXXXXXXXXXRGTRISTVPAXXXXXXXXXXXXXXFNS--GVAQSSIIDH 342
             I SLP E              T IS  P                +S   ++Q SII++
Sbjct: 241 LSISSLPRELEDLKRLKYLLLSNTAISEAPRELFSSMDSLEVLDLSSSLKKISQGSIINN 300

Query: 343 -LGCLEGRLVALGIKASSLLDLRKL 414
            L  L+GRL  LGI A S  D+ KL
Sbjct: 301 NLDGLKGRLKGLGINACSRRDIDKL 325


>ref|XP_004984978.1| disease resistance protein RPS2 [Setaria italica]
 gb|KQK91650.1| hypothetical protein SETIT_034139mg [Setaria italica]
          Length = 913

 Score = 56.6 bits (135), Expect = 2e-06
 Identities = 40/133 (30%), Positives = 56/133 (42%), Gaps = 8/133 (6%)
 Frame = +1

Query: 40  KSMSILKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTRIKSLPEEXXXXXXXX 219
           + +  ++H   L YLDL D      FP+EIC L  LEYLNLS+ RI SLP E        
Sbjct: 559 RMLQAIQHFTKLTYLDLEDTGIQDAFPMEICCLVNLEYLNLSKNRILSLPMELGNLSQLK 618

Query: 220 XXXXRGTRI--STVPAXXXXXXXXXXXXXXFNSGVAQ------SSIIDHLGCLEGRLVAL 375
               R       T+P               F + +        S +I+ L     R+ +L
Sbjct: 619 YFYLRDNYYIQITIPPGLVSRLGKLQVLEVFTASIVSVADDYVSPVIEDLESSGARMASL 678

Query: 376 GIKASSLLDLRKL 414
           GI   S+ D+ +L
Sbjct: 679 GIWLDSIRDVERL 691


>ref|XP_003558328.1| PREDICTED: probable disease resistance protein At1g61300
           [Brachypodium distachyon]
 gb|KQK22526.1| hypothetical protein BRADI_1g67840v3 [Brachypodium distachyon]
          Length = 926

 Score = 56.6 bits (135), Expect = 2e-06
 Identities = 41/133 (30%), Positives = 57/133 (42%), Gaps = 8/133 (6%)
 Frame = +1

Query: 40  KSMSILKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTRIKSLPEEXXXXXXXX 219
           K +  ++H   L YLDL D   +  FP+EIC L  LEYLNLSR RI SLP E        
Sbjct: 570 KMLQAIQHFTRLTYLDLEDTGIVDAFPMEICCLVSLEYLNLSRNRILSLPMELGNLSGLK 629

Query: 220 XXXXRGTRI--STVPAXXXXXXXXXXXXXXFNSGVAQ------SSIIDHLGCLEGRLVAL 375
               R       T+PA              F + +        + +ID L      + +L
Sbjct: 630 YLHMRDNYYIQITIPAGLISRLGKLQVLELFTASIVSVADDYVAPVIDDLESSGASVASL 689

Query: 376 GIKASSLLDLRKL 414
           GI   +  D+++L
Sbjct: 690 GIWLDNTRDVQRL 702


>gb|OEL16537.1| Disease resistance protein RPS2 [Dichanthelium oligosanthes]
          Length = 914

 Score = 56.2 bits (134), Expect = 2e-06
 Identities = 31/64 (48%), Positives = 40/64 (62%), Gaps = 2/64 (3%)
 Frame = +1

Query: 10  TLILRGNLWL--KSMSILKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTRIKS 183
           +L+L+GN  L  + +  ++H   L YLDL D      FP+EIC L  LEYLNLS+ RI S
Sbjct: 548 SLMLQGNRALPKRMLQAIQHFTKLTYLDLEDTGIQDAFPMEICCLVNLEYLNLSKNRILS 607

Query: 184 LPEE 195
           LP E
Sbjct: 608 LPME 611


>dbj|BAF11498.2| Os03g0254000 [Oryza sativa Japonica Group]
          Length = 558

 Score = 55.8 bits (133), Expect = 3e-06
 Identities = 45/145 (31%), Positives = 63/145 (43%), Gaps = 10/145 (6%)
 Frame = +1

Query: 10  TLILRGNLWLKSMSI--LKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTRIKS 183
           TL+L+ N  L    I  ++H   L YLD+ +   +  FP+EIC L  LEYLNLS+ RI S
Sbjct: 189 TLMLQCNRALPKRMIQAIQHFTRLTYLDMEETGIVDAFPMEICCLVNLEYLNLSKNRILS 248

Query: 184 LPEEXXXXXXXXXXXXRGTRI--STVPAXXXXXXXXXXXXXXFNSGVAQ------SSIID 339
           LP E            R       T+PA              F + +        + +ID
Sbjct: 249 LPMELSNLSQLKYLYLRDNYYIQITIPAGLISRLGKLQVLELFTASIVSIADDYIAPVID 308

Query: 340 HLGCLEGRLVALGIKASSLLDLRKL 414
            L     +L ALG+   S  D+ +L
Sbjct: 309 DLESSGAQLTALGLWLDSTRDVARL 333


>gb|ESQ47302.1| hypothetical protein EUTSA_v10028060mg [Eutrema salsugineum]
          Length = 837

 Score = 55.8 bits (133), Expect = 3e-06
 Identities = 33/64 (51%), Positives = 42/64 (65%), Gaps = 1/64 (1%)
 Frame = +1

Query: 1   NLTTLILRGN-LWLKSMSILKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTRI 177
           NL+TL+LR N L + S    + +PSL  LDLS+   +   P EIC L+ LEYLNLS T I
Sbjct: 534 NLSTLLLRDNKLKIISGEFFRFMPSLVVLDLSENTRLCALPEEICSLSSLEYLNLSWTSI 593

Query: 178 KSLP 189
           +SLP
Sbjct: 594 ESLP 597


>ref|XP_009147820.1| PREDICTED: probable disease resistance protein At1g15890 [Brassica
           rapa]
          Length = 852

 Score = 55.8 bits (133), Expect = 3e-06
 Identities = 34/65 (52%), Positives = 42/65 (64%), Gaps = 2/65 (3%)
 Frame = +1

Query: 1   NLTTLILRGNLWLKSM--SILKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTR 174
           NL+TL L  N+ LK +     + +PSL  LDLS  + + E P EIC L  L+YLNLSRTR
Sbjct: 537 NLSTLFLGDNM-LKVIPGEFFQFMPSLVVLDLSRNLILLELPEEICSLISLQYLNLSRTR 595

Query: 175 IKSLP 189
           I SLP
Sbjct: 596 ISSLP 600


>gb|ACP30592.1| disease resistance protein [Brassica rapa subsp. pekinensis]
          Length = 852

 Score = 55.8 bits (133), Expect = 3e-06
 Identities = 34/65 (52%), Positives = 42/65 (64%), Gaps = 2/65 (3%)
 Frame = +1

Query: 1   NLTTLILRGNLWLKSM--SILKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTR 174
           NL+TL L  N+ LK +     + +PSL  LDLS  + + E P EIC L  L+YLNLSRTR
Sbjct: 537 NLSTLFLGDNM-LKVIPGEFFQFMPSLVVLDLSRNLILLELPEEICSLISLQYLNLSRTR 595

Query: 175 IKSLP 189
           I SLP
Sbjct: 596 ISSLP 600


>ref|XP_024014671.1| LOW QUALITY PROTEIN: probable disease resistance protein At1g15890
           [Eutrema salsugineum]
          Length = 864

 Score = 55.8 bits (133), Expect = 3e-06
 Identities = 33/64 (51%), Positives = 42/64 (65%), Gaps = 1/64 (1%)
 Frame = +1

Query: 1   NLTTLILRGN-LWLKSMSILKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTRI 177
           NL+TL+LR N L + S    + +PSL  LDLS+   +   P EIC L+ LEYLNLS T I
Sbjct: 539 NLSTLLLRDNKLKIISGEFFRFMPSLVVLDLSENTRLCALPEEICSLSSLEYLNLSWTSI 598

Query: 178 KSLP 189
           +SLP
Sbjct: 599 ESLP 602


>gb|EEE58713.1| hypothetical protein OsJ_10170 [Oryza sativa Japonica Group]
          Length = 866

 Score = 55.8 bits (133), Expect = 3e-06
 Identities = 45/145 (31%), Positives = 63/145 (43%), Gaps = 10/145 (6%)
 Frame = +1

Query: 10  TLILRGNLWLKSMSI--LKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTRIKS 183
           TL+L+ N  L    I  ++H   L YLD+ +   +  FP+EIC L  LEYLNLS+ RI S
Sbjct: 528 TLMLQCNRALPKRMIQAIQHFTRLTYLDMEETGIVDAFPMEICCLVNLEYLNLSKNRILS 587

Query: 184 LPEEXXXXXXXXXXXXRGTRI--STVPAXXXXXXXXXXXXXXFNSGVAQ------SSIID 339
           LP E            R       T+PA              F + +        + +ID
Sbjct: 588 LPMELSNLSQLKYLYLRDNYYIQITIPAGLISRLGKLQVLELFTASIVSIADDYIAPVID 647

Query: 340 HLGCLEGRLVALGIKASSLLDLRKL 414
            L     +L ALG+   S  D+ +L
Sbjct: 648 DLESSGAQLTALGLWLDSTRDVARL 672


>ref|XP_018457909.1| PREDICTED: LOW QUALITY PROTEIN: probable disease resistance protein
           At1g61300 [Raphanus sativus]
          Length = 875

 Score = 55.8 bits (133), Expect = 3e-06
 Identities = 30/64 (46%), Positives = 45/64 (70%), Gaps = 2/64 (3%)
 Frame = +1

Query: 4   LTTLILRGNLWLKSMS--ILKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTRI 177
           +TTL+L+ N+ LK++S  +++++  L  LDLS  I +KE P +I  L  L+YL+LS TRI
Sbjct: 531 ITTLLLQDNINLKNLSGEVIQYMQKLLVLDLSSNISIKELPEQISELTSLQYLDLSDTRI 590

Query: 178 KSLP 189
           K LP
Sbjct: 591 KQLP 594


>ref|XP_010654604.1| PREDICTED: probable disease resistance protein At5g63020 [Vitis
           vinifera]
 ref|XP_010654605.1| PREDICTED: probable disease resistance protein At5g63020 [Vitis
           vinifera]
          Length = 885

 Score = 55.8 bits (133), Expect = 3e-06
 Identities = 34/67 (50%), Positives = 44/67 (65%), Gaps = 2/67 (2%)
 Frame = +1

Query: 1   NLTTLILRGNLWLKSMSI--LKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTR 174
           NL TLILR N  +KS+ I   + +P ++ LDLSD   + E P+EIC L  LEYLNL+ T 
Sbjct: 537 NLQTLILR-NSNMKSLPIGFFQSMPVIRVLDLSDNRNLVELPLEICRLESLEYLNLTGTS 595

Query: 175 IKSLPEE 195
           IK +P E
Sbjct: 596 IKRMPIE 602


>gb|EEC74881.1| hypothetical protein OsI_10792 [Oryza sativa Indica Group]
          Length = 916

 Score = 55.8 bits (133), Expect = 3e-06
 Identities = 45/145 (31%), Positives = 63/145 (43%), Gaps = 10/145 (6%)
 Frame = +1

Query: 10  TLILRGNLWLKSMSI--LKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTRIKS 183
           TL+L+ N  L    I  ++H   L YLD+ +   +  FP+EIC L  LEYLNLS+ RI S
Sbjct: 547 TLMLQCNRALPKRMIQAIQHFTRLTYLDMEETGIVDAFPMEICCLVNLEYLNLSKNRILS 606

Query: 184 LPEEXXXXXXXXXXXXRGTRI--STVPAXXXXXXXXXXXXXXFNSGVAQ------SSIID 339
           LP E            R       T+PA              F + +        + +ID
Sbjct: 607 LPMELSNLSQLKYLYLRDNYYIQITIPAGLISRLGKLQVLELFTASIVSIADDYIAPVID 666

Query: 340 HLGCLEGRLVALGIKASSLLDLRKL 414
            L     +L ALG+   S  D+ +L
Sbjct: 667 DLESSGAQLTALGLWLDSTRDVARL 691


>ref|XP_015628423.1| PREDICTED: probable disease resistance protein At1g61300 [Oryza
           sativa Japonica Group]
 gb|ABF95015.1| NB-ARC domain containing protein, expressed [Oryza sativa Japonica
           Group]
 dbj|BAH01387.1| unnamed protein product [Oryza sativa Japonica Group]
 dbj|BAS83308.1| Os03g0254000 [Oryza sativa Japonica Group]
          Length = 916

 Score = 55.8 bits (133), Expect = 3e-06
 Identities = 45/145 (31%), Positives = 63/145 (43%), Gaps = 10/145 (6%)
 Frame = +1

Query: 10  TLILRGNLWLKSMSI--LKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTRIKS 183
           TL+L+ N  L    I  ++H   L YLD+ +   +  FP+EIC L  LEYLNLS+ RI S
Sbjct: 547 TLMLQCNRALPKRMIQAIQHFTRLTYLDMEETGIVDAFPMEICCLVNLEYLNLSKNRILS 606

Query: 184 LPEEXXXXXXXXXXXXRGTRI--STVPAXXXXXXXXXXXXXXFNSGVAQ------SSIID 339
           LP E            R       T+PA              F + +        + +ID
Sbjct: 607 LPMELSNLSQLKYLYLRDNYYIQITIPAGLISRLGKLQVLELFTASIVSIADDYIAPVID 666

Query: 340 HLGCLEGRLVALGIKASSLLDLRKL 414
            L     +L ALG+   S  D+ +L
Sbjct: 667 DLESSGAQLTALGLWLDSTRDVARL 691


>emb|CBI36041.3| unnamed protein product, partial [Vitis vinifera]
          Length = 1123

 Score = 55.8 bits (133), Expect = 3e-06
 Identities = 34/67 (50%), Positives = 44/67 (65%), Gaps = 2/67 (2%)
 Frame = +1

Query: 1   NLTTLILRGNLWLKSMSI--LKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTR 174
           NL TLILR N  +KS+ I   + +P ++ LDLSD   + E P+EIC L  LEYLNL+ T 
Sbjct: 800 NLQTLILR-NSNMKSLPIGFFQSMPVIRVLDLSDNRNLVELPLEICRLESLEYLNLTGTS 858

Query: 175 IKSLPEE 195
           IK +P E
Sbjct: 859 IKRMPIE 865


>gb|AFE85505.1| putative CC-NBS-LRR disease resistance protein, partial [Zingiber
           zerumbet]
          Length = 759

 Score = 55.1 bits (131), Expect = 5e-06
 Identities = 36/87 (41%), Positives = 47/87 (54%), Gaps = 2/87 (2%)
 Frame = +1

Query: 4   LTTLILRGNLWLKSM--SILKHLPSLKYLDLSDCICMKEFPIEICGLNKLEYLNLSRTRI 177
           L+ LIL GN  L+++  S+   +P L YLDLSDC  + E P+EI  L +L+YLNLS   I
Sbjct: 670 LSMLILLGNGRLETIPPSLFASMPHLTYLDLSDCH-ITELPMEISSLTELQYLNLSSNPI 728

Query: 178 KSLPEEXXXXXXXXXXXXRGTRISTVP 258
             LP E            R T +  VP
Sbjct: 729 TRLPIEFGCLSKLEYLLLRDTNLKIVP 755


Top