BLASTX nr result
ID: Ophiopogon22_contig00012246
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ophiopogon22_contig00012246
(4157 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_020267901.1| E3 ubiquitin-protein ligase UPL4-like [Aspar... 1625 0.0
gb|PNS98590.1| hypothetical protein POPTR_016G085200v3 [Populus ... 1094 0.0
gb|PNS98589.1| hypothetical protein POPTR_016G085200v3 [Populus ... 1071 0.0
ref|XP_022939346.1| E3 ubiquitin-protein ligase UPL4-like isofor... 997 0.0
gb|PIA45462.1| hypothetical protein AQUCO_01700766v1, partial [A... 996 0.0
gb|PIA45461.1| hypothetical protein AQUCO_01700766v1 [Aquilegia ... 996 0.0
ref|XP_023005685.1| E3 ubiquitin-protein ligase UPL4-like isofor... 992 0.0
ref|XP_023539461.1| E3 ubiquitin-protein ligase UPL4-like isofor... 991 0.0
ref|XP_023527254.1| E3 ubiquitin-protein ligase UPL4-like isofor... 986 0.0
ref|XP_022983502.1| E3 ubiquitin-protein ligase UPL4-like isofor... 984 0.0
ref|XP_023539460.1| E3 ubiquitin-protein ligase UPL4-like isofor... 983 0.0
ref|XP_023539462.1| E3 ubiquitin-protein ligase UPL4-like isofor... 983 0.0
ref|XP_023539459.1| E3 ubiquitin-protein ligase UPL4-like isofor... 983 0.0
ref|XP_022934795.1| E3 ubiquitin-protein ligase UPL4-like isofor... 979 0.0
ref|XP_023527253.1| E3 ubiquitin-protein ligase UPL4-like isofor... 976 0.0
ref|XP_023527252.1| E3 ubiquitin-protein ligase UPL4-like isofor... 976 0.0
ref|XP_022983501.1| E3 ubiquitin-protein ligase UPL4-like isofor... 973 0.0
ref|XP_022983499.1| E3 ubiquitin-protein ligase UPL4-like isofor... 973 0.0
ref|XP_022934794.1| E3 ubiquitin-protein ligase UPL4-like isofor... 967 0.0
ref|XP_022934791.1| E3 ubiquitin-protein ligase UPL4-like isofor... 967 0.0
>ref|XP_020267901.1| E3 ubiquitin-protein ligase UPL4-like [Asparagus officinalis]
gb|ONK67834.1| uncharacterized protein A4U43_C05F4270 [Asparagus officinalis]
Length = 1400
Score = 1625 bits (4207), Expect = 0.0
Identities = 859/1245 (68%), Positives = 976/1245 (78%), Gaps = 12/1245 (0%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
N C++VPEDC S VMDAVP LC LLDYQD+KLVES A LM+IVENF HSS+LLDELCKH
Sbjct: 169 NACRKVPEDCLSTVMDAVPTLCKLLDYQDQKLVESAATSLMKIVENFSHSSDLLDELCKH 228
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
GVVKQSVKLISP S +ALSE T IGMIELL RLAN SLL++ LYE NIN+IL ++LR
Sbjct: 229 GVVKQSVKLISPCSTVALSERTNIGMIELLARLANSSLLAVSILYELNINSILGNLLRRP 288
Query: 363 DLSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQF 542
LS SH ED SRDQ++ ALKLLNQLIPP+A D++DIQLV GK +II +EPKLSFQF
Sbjct: 289 HLSHSAHNSHSEDDSRDQIFAALKLLNQLIPPMACDEKDIQLVSGKNEIIANEPKLSFQF 348
Query: 543 AVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSRK 722
A ILPALIQAV SGA+ I Y VSVI NY L TPD+LR LIKNAN+PKFLA LLS+K
Sbjct: 349 AEDILPALIQAVNSGASTRICYGSVSVILNYFLAVTPDVLRVLIKNANIPKFLASLLSQK 408
Query: 723 DHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQSQLMDQQSSNLGD 902
+HH+LIP+L +VE+IM++LPGELL FVKEGVSHAI V+SKQ+ Q ++ GD
Sbjct: 409 NHHLLIPSLLIVEVIMRELPGELLKPFVKEGVSHAINVISKQENWSQ--------TDTGD 460
Query: 903 TDERTAADLSRCLCYAFD-XXXXXXXXXKMCNLPRHTLLPLAQRVMSTFFXXXXXXXXXX 1079
TD++T A+ RCLCYAFD + C R L PLAQR+MSTFF
Sbjct: 461 TDDQTIANPPRCLCYAFDPCSSPSSSESRTCRQRRCDLSPLAQRLMSTFFTETLISSMVF 520
Query: 1080 XXXXQKLKACCIALDKNVDSALTNCAEREDYLSQMIVEAVTALNEGESISTFEFIESGFI 1259
+KLK+CC+ +D NV SAL N +E+E+YL+QM+ E V LNEGES+STFEFIESG
Sbjct: 521 SENLRKLKSCCMEIDNNVHSALPNSSEKEEYLTQMLGELVKVLNEGESVSTFEFIESGIP 580
Query: 1260 RFLAHYLTNGDYLHRALGSDMSFDYLTVLKRLQTFSFTLLSGHSGQHFPLASLVQHLQNA 1439
RFL HYLTNGD+ R + S+ + Y TVLKR QTF+ LLSG S Q FPL SLV+HLQNA
Sbjct: 581 RFLVHYLTNGDHFPRGVYSNKTEAYHTVLKRFQTFTSMLLSGQSWQLFPLTSLVRHLQNA 640
Query: 1440 LSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLCLYDNVLTVDIS 1619
LS+LE FP++LS+ + R DI A+ T HPCLQV+FV EE ET+LC Y+NVLTVD S
Sbjct: 641 LSSLESFPIILSSGYRPTPRRADISAEKPTVHPCLQVNFVTEERETELCNYNNVLTVDFS 700
Query: 1620 SSYDAIEEFLWPXXXXXXXXXXXXXXNI---KQSSRKVDSKEQVGSSTTQNNEGDITPKL 1790
SS+D IEEFLWP ++S++ VDS+EQ +S QNNE D+ PKL
Sbjct: 701 SSWDTIEEFLWPKVKKKMSVTKSAQETRHPGEKSAQIVDSQEQGENSMLQNNE-DVKPKL 759
Query: 1791 IFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYP---- 1958
+FLLDGKQLD S+TLYQAILQDKIN+DP II++++FWNEVH V R AK+LQL P
Sbjct: 760 VFLLDGKQLDHSVTLYQAILQDKINQDPAIIINREFWNEVHGVKFRKAKELQLRLPLKKG 819
Query: 1959 --QISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGL 2132
Q+ AD QS+LS D L N SFFSSMLL+ELP KLERSNPAYD+L++LKILEG+
Sbjct: 820 SFQVFADTSQSSLSCDTAGL-CRQNLSFFSSMLLSELPCKLERSNPAYDILYILKILEGI 878
Query: 2133 NRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLEQQMHDHLALST 2312
N SFQL SNE+SNAFAEG L N DDLKVIA SLPQTEFI+SKL SKLEQQMHDHL LST
Sbjct: 879 NGFSFQLSSNEKSNAFAEGRLNNADDLKVIAPSLPQTEFISSKLNSKLEQQMHDHLVLST 938
Query: 2313 ATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEISTINDRRSLSASL 2492
TMPLWC Q++V+ PFLFS EARWKYF LN G+SRN NSEK EIS INDRRSLS SL
Sbjct: 939 TTMPLWCTQIMVSFPFLFSLEARWKYFFLNTLGISRNHMQNSEKNEISNINDRRSLSTSL 998
Query: 2493 PRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVG 2672
RKKFKVHR+NIL SA KMM WYA KKVLLEI F+NEVGTGLGPTLEFYTLVSH QKVG
Sbjct: 999 RRKKFKVHRDNILGSAVKMMNWYASKKVLLEIVFNNEVGTGLGPTLEFYTLVSHELQKVG 1058
Query: 2673 MGMWRGDHTSTAKRL--RTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFF 2846
MGMWRGDHTS+ +L R SMVVSDS F+ HSGLFPRPWS ++ NGVSFSKVLKNFF
Sbjct: 1059 MGMWRGDHTSSGTKLHSRKSMVVSDSSFVRAHSGLFPRPWSIRVESLNGVSFSKVLKNFF 1118
Query: 2847 LLGQLTARAIQDGRILDLPFSSAFYKLMLEKDLDIYDIQSFDPELGRTMIEFQALAKRKG 3026
LLGQL ARAIQDGRILD FS AFYKL+LEKDLDIYDIQSFDPELGRT+IEFQAL KRK
Sbjct: 1119 LLGQLVARAIQDGRILDFTFSRAFYKLVLEKDLDIYDIQSFDPELGRTLIEFQALVKRKA 1178
Query: 3027 FLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSL 3206
FLE SGE S D FRN RIE+LCLDF+LPGYPDY F S DSKMVN+SNL EY+SL
Sbjct: 1179 FLE--SGELSEDGSALSFRNARIEDLCLDFALPGYPDYAFGS-SDSKMVNMSNLGEYISL 1235
Query: 3207 VVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINF 3386
VVDATV SGISRQLESFKSGF++VFPIKALQIFTA+ELE+++ GEQDTWD E Q+HI F
Sbjct: 1236 VVDATVGSGISRQLESFKSGFNEVFPIKALQIFTAQELEQIVSGEQDTWDNNEFQDHIKF 1295
Query: 3387 DHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDN 3566
DHGYSSTS A+ FLE+IQEF+ RQRRAFLLFVTGAPRLPPGGLAAL+PKLTIVCKHCDN
Sbjct: 1296 DHGYSSTSPTAIMFLEVIQEFEVRQRRAFLLFVTGAPRLPPGGLAALRPKLTIVCKHCDN 1355
Query: 3567 DADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 3701
DADMELPSVMTCANYLKLPPYSSKERM+EKLLYAI EGQGAFHLS
Sbjct: 1356 DADMELPSVMTCANYLKLPPYSSKERMKEKLLYAIMEGQGAFHLS 1400
>gb|PNS98590.1| hypothetical protein POPTR_016G085200v3 [Populus trichocarpa]
Length = 1504
Score = 1094 bits (2829), Expect = 0.0
Identities = 605/1256 (48%), Positives = 811/1256 (64%), Gaps = 23/1256 (1%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
N+CK++P + FS M+AVP+LCNLL Y+DR+LVE+ A+CL++I E SSE+LDELCKH
Sbjct: 256 NICKKLPSENFSPFMEAVPILCNLLQYEDRQLVENVAICLIKIAERVSQSSEMLDELCKH 315
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G++ Q+ L+ +S LS+ Y G+I LLV+L++ S+++ R+LYE NI++IL+ +
Sbjct: 316 GLINQATHLVQLNSRTTLSQPVYNGLIGLLVKLSSGSIVAFRTLYELNISSILKDLFATY 375
Query: 363 DLSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQF 542
DLS H D +QV+ LKLLN+L+P VAR+ QLVL KE + + P L +F
Sbjct: 376 DLSHGISSPHVIDGQGNQVHEVLKLLNELLPTVARNQDAQQLVLDKEAFLANHPDLLHKF 435
Query: 543 AVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSRK 722
I+P+LIQ V SGAN + Y C+ VI V ++ DML L+KN N FLAG+L+RK
Sbjct: 436 GSDIIPSLIQVVNSGANLYVCYGCLYVINKLVYLSKSDMLLELLKNTNFSSFLAGVLTRK 495
Query: 723 DHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQSQLMDQQSSN--- 893
DHHVL+ AL + E I++KLP +NSF+KEGV AI L + C SQL+ +
Sbjct: 496 DHHVLMLALQITETILQKLPDVFVNSFIKEGVFFAIDGLLVPEKC--SQLIFPACNGIHL 553
Query: 894 -LGDTDERTAADLSRCLCYAFDXXXXXXXXXK-MCNLPRHTLLPLAQRVMSTFFXXXXXX 1067
L + ++ + RCLCYAFD C L + T+ L + + ++F
Sbjct: 554 PLNSNQKSSSKVVMRCLCYAFDTGQSLSASETGTCKLEKDTVENLGKHIRISYFALESCD 613
Query: 1068 XXXXXXXX-QKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAVTALNEGESISTFE 1238
QKL+A L N+ + +C + E+ ++ + + L+ E +STFE
Sbjct: 614 SEKGLTDILQKLRALSAELSDLMNMSVKIGSCTQDEEKCYSILCQIMEKLDGREPVSTFE 673
Query: 1239 FIESGFIRFLAHYLTNGDYLHRALGSDMSFD-YLTVLKRLQTFSFTLLSGHSGQHFPLAS 1415
FIESG ++ L +YL NG YL + +FD + V KR + F+ L S + PL++
Sbjct: 674 FIESGIVKILVNYLFNGKYLREKVEPQSTFDDFYVVEKRFEVFARLLSSSDLSEESPLSA 733
Query: 1416 LVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLCLY- 1592
L+Q LQ ALS+ E FPV+LS A+K +S IP T +PCL+V FV EGET LC Y
Sbjct: 734 LIQKLQGALSSSENFPVILSHASKYRSSFAIIPNGRRTSYPCLRVRFVRGEGETCLCNYS 793
Query: 1593 DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVDSKEQVGSST------ 1754
++ +TVD SS + IE FL P + + V ++ V S +
Sbjct: 794 EDPVTVDPLSSVNTIEGFLSPKVRIKGTEQIESAAQALEPAENVSVEDIVQSPSCADDST 853
Query: 1755 -----TQNNEGDITPKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRV 1919
T + GD PKL+F L+G+QLDR++TLYQAILQ K+ D +I + K W +VH +
Sbjct: 854 KSHCPTSCSNGDAMPKLVFYLEGQQLDRTLTLYQAILQQKVKADHEINSTAKLWTQVHTL 913
Query: 1920 TLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYD 2099
T R A + Q Q++ D+ + + + +FFSSM ELP L++S+P D
Sbjct: 914 TYRIAVDTRDDNTQDCPSMAQNSSILDQA-VAFMQHPAFFSSMFNCELPSDLDKSSPTND 972
Query: 2100 MLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLE 2279
+LF+LK LEGLNR F L S+ER +AFAEG ++N D+L+V A+ + Q EF++SKLT KLE
Sbjct: 973 ILFLLKSLEGLNRFIFHLMSHERIHAFAEGLIDNLDNLRVAARPVAQNEFVSSKLTEKLE 1032
Query: 2280 QQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEIST 2459
QQM D LA+S MP+WC QL+ +C FLFSFE R KYF L+ FG + Q S
Sbjct: 1033 QQMRDSLAVSMGGMPVWCNQLMNSCSFLFSFETRCKYFQLSAFGCQQIQIQPSSHNNSGV 1092
Query: 2460 INDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFY 2639
+ DR + SL RKKF V R+ +L SAA+MM YA KV +E+ ++ EVGTGLGPTLEFY
Sbjct: 1093 LRDRLPSAGSLSRKKFIVLRDQVLESAAQMMDRYAHLKVPIEVVYNEEVGTGLGPTLEFY 1152
Query: 2640 TLVSHAFQKVGMGMWRGDHTS--TAKRLRTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNG 2813
TLVS FQK G+GMWR DH S T + L+ SG + GLFPRPWS +D S+G
Sbjct: 1153 TLVSKEFQKSGIGMWREDHISFPTIENLQAEY----SGIVKSPFGLFPRPWSPTVDASDG 1208
Query: 2814 VSFSKVLKNFFLLGQLTARAIQDGRILDLPFSSAFYKLMLEKDLDIYDIQSFDPELGRTM 2993
V FS+V+K FFLLGQ+ A+A+QDGR+LDLPF+ FYKL+L+++L++YDIQSFDPELGRT+
Sbjct: 1209 VQFSEVIKKFFLLGQIVAKALQDGRVLDLPFAKVFYKLILQQELNLYDIQSFDPELGRTL 1268
Query: 2994 IEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMV 3173
+EFQAL RK + E+S D F NTRIE+LCLDF+LPGY DY+ S D K+V
Sbjct: 1269 LEFQALVNRKKNMGLVIVENSSSTQDACFWNTRIEDLCLDFTLPGYSDYILSFDEDHKIV 1328
Query: 3174 NISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDTW 3353
N+ NLE YVS +VDAT+ +GISRQ+E+FKSGF++VFPIK L IFT EELERLLCGE+D W
Sbjct: 1329 NMDNLEVYVSHIVDATIHTGISRQVEAFKSGFNQVFPIKHLMIFTEEELERLLCGERDFW 1388
Query: 3354 DIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKP 3533
EL +HI FDHGY+++S P + LEII+EF+ QRR+FL FVTGAPRLP GGLA+L P
Sbjct: 1389 AFNELLDHIKFDHGYTASSPPIVNLLEIIKEFEYEQRRSFLQFVTGAPRLPTGGLASLNP 1448
Query: 3534 KLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 3701
KLTIV KHC N D++LPSVMTCANYLKLPPYSSK++M+EKLLYAI EGQG+FHLS
Sbjct: 1449 KLTIVRKHCSNCEDVDLPSVMTCANYLKLPPYSSKDKMKEKLLYAITEGQGSFHLS 1504
>gb|PNS98589.1| hypothetical protein POPTR_016G085200v3 [Populus trichocarpa]
Length = 1512
Score = 1071 bits (2770), Expect = 0.0
Identities = 601/1269 (47%), Positives = 806/1269 (63%), Gaps = 36/1269 (2%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
N+CK++P + FS M+AVP+LCNLL Y+DR+LVE+ A+CL++I E SSE+LDELCKH
Sbjct: 256 NICKKLPSENFSPFMEAVPILCNLLQYEDRQLVENVAICLIKIAERVSQSSEMLDELCKH 315
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G++ Q+ L+ +S LS+ Y G+I LLV+L++ S+++ R+LYE NI++IL+ +
Sbjct: 316 GLINQATHLVQLNSRTTLSQPVYNGLIGLLVKLSSGSIVAFRTLYELNISSILKDLFATY 375
Query: 363 DLSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQF 542
DLS H D +QV+ LKLLN+L+P VAR+ QLVL KE + + P L +F
Sbjct: 376 DLSHGISSPHVIDGQGNQVHEVLKLLNELLPTVARNQDAQQLVLDKEAFLANHPDLLHKF 435
Query: 543 AVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSRK 722
I+P+LIQ V SGAN + Y C+ VI V ++ DML L+KN N FLAG+L+RK
Sbjct: 436 GSDIIPSLIQVVNSGANLYVCYGCLYVINKLVYLSKSDMLLELLKNTNFSSFLAGVLTRK 495
Query: 723 DHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQSQLMDQQSSN--- 893
DHHVL+ AL + E I++KLP +NSF+KEGV AI L + C SQL+ +
Sbjct: 496 DHHVLMLALQITETILQKLPDVFVNSFIKEGVFFAIDGLLVPEKC--SQLIFPACNGIHL 553
Query: 894 -LGDTDERTAADLSRCLCYAFDXXXXXXXXXK-MCNLPRHTLLPLAQRVMSTFFXXXXXX 1067
L + ++ + RCLCYAFD C L + T+ L + + ++F
Sbjct: 554 PLNSNQKSSSKVVMRCLCYAFDTGQSLSASETGTCKLEKDTVENLGKHIRISYFALESCD 613
Query: 1068 XXXXXXXX-QKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAVTALNEGESISTFE 1238
QKL+A L N+ + +C + E+ ++ + + L+ E +STFE
Sbjct: 614 SEKGLTDILQKLRALSAELSDLMNMSVKIGSCTQDEEKCYSILCQIMEKLDGREPVSTFE 673
Query: 1239 FIESGFIRFLAHYLTNGDYLHRALGSDMSFD-YLTVLKRLQTFSFTLLSGHSGQHFPLAS 1415
FIESG ++ L +YL NG YL + +FD + V KR + F+ L S + PL++
Sbjct: 674 FIESGIVKILVNYLFNGKYLREKVEPQSTFDDFYVVEKRFEVFARLLSSSDLSEESPLSA 733
Query: 1416 LVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLCLY- 1592
L+Q LQ ALS+ E FPV+LS A+K +S IP T +PCL+V FV EGET LC Y
Sbjct: 734 LIQKLQGALSSSENFPVILSHASKYRSSFAIIPNGRRTSYPCLRVRFVRGEGETCLCNYS 793
Query: 1593 DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVDSKEQVGSST------ 1754
++ +TVD SS + IE FL P + + V ++ V S +
Sbjct: 794 EDPVTVDPLSSVNTIEGFLSPKVRIKGTEQIESAAQALEPAENVSVEDIVQSPSCADDST 853
Query: 1755 -----TQNNEGDITPKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRV 1919
T + GD PKL+F L+G+QLDR++TLYQAILQ K+ D +I + K W +VH +
Sbjct: 854 KSHCPTSCSNGDAMPKLVFYLEGQQLDRTLTLYQAILQQKVKADHEINSTAKLWTQVHTL 913
Query: 1920 TLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYD 2099
T R A + Q Q++ D+ + + + +FFSSM ELP L++S+P D
Sbjct: 914 TYRIAVDTRDDNTQDCPSMAQNSSILDQA-VAFMQHPAFFSSMFNCELPSDLDKSSPTND 972
Query: 2100 MLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLE 2279
+LF+LK LEGLNR F L S+ER +AFAEG ++N D+L+V A+ + Q EF++SKLT KLE
Sbjct: 973 ILFLLKSLEGLNRFIFHLMSHERIHAFAEGLIDNLDNLRVAARPVAQNEFVSSKLTEKLE 1032
Query: 2280 QQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEIST 2459
QQM D LA+S MP+WC QL+ +C FLFSFE R KYF L+ FG + Q S
Sbjct: 1033 QQMRDSLAVSMGGMPVWCNQLMNSCSFLFSFETRCKYFQLSAFGCQQIQIQPSSHNNSGV 1092
Query: 2460 INDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFY 2639
+ DR + SL RKKF V R+ +L SAA+MM YA KV +E+ ++ EVGTGLGPTLEFY
Sbjct: 1093 LRDRLPSAGSLSRKKFIVLRDQVLESAAQMMDRYAHLKVPIEVVYNEEVGTGLGPTLEFY 1152
Query: 2640 TLVSHAFQKVGMGMWRGDHTS--TAKRLRTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNG 2813
TLVS FQK G+GMWR DH S T + L+ SG + GLFPRPWS +D S+G
Sbjct: 1153 TLVSKEFQKSGIGMWREDHISFPTIENLQAEY----SGIVKSPFGLFPRPWSPTVDASDG 1208
Query: 2814 VSFSKVLKNFFLLGQLTARAIQDGRILDLPFSSAFYKLMLEKDLDIYDIQSFDPELGRTM 2993
V FS+V+K FFLLGQ+ A+A+QDGR+LDLPF+ FYKL+L+++L++YDIQSFDPELGRT+
Sbjct: 1209 VQFSEVIKKFFLLGQIVAKALQDGRVLDLPFAKVFYKLILQQELNLYDIQSFDPELGRTL 1268
Query: 2994 IEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMV 3173
+EFQAL RK + E+S D F NTRIE+LCLDF+LPGY DY+ S D K+V
Sbjct: 1269 LEFQALVNRKKNMGLVIVENSSSTQDACFWNTRIEDLCLDFTLPGYSDYILSFDEDHKIV 1328
Query: 3174 NISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDTW 3353
N+ NLE YVS +VDAT+ +GISRQ+E+FKSGF++VFPIK L IFT EELERLLCGE+D W
Sbjct: 1329 NMDNLEVYVSHIVDATIHTGISRQVEAFKSGFNQVFPIKHLMIFTEEELERLLCGERDFW 1388
Query: 3354 DIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKP 3533
EL +HI FDHGY+++S P + EF+ QRR+FL FVTGAPRLP GGLA+L P
Sbjct: 1389 AFNELLDHIKFDHGYTASSPPIVNV-----EFEYEQRRSFLQFVTGAPRLPTGGLASLNP 1443
Query: 3534 KLTIVCK-------------HCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIR 3674
KLTIV K HC N D++LPSVMTCANYLKLPPYSSK++M+EKLLYAI
Sbjct: 1444 KLTIVRKSLFNHLSSWCSLQHCSNCEDVDLPSVMTCANYLKLPPYSSKDKMKEKLLYAIT 1503
Query: 3675 EGQGAFHLS 3701
EGQG+FHLS
Sbjct: 1504 EGQGSFHLS 1512
>ref|XP_022939346.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita moschata]
Length = 1485
Score = 997 bits (2578), Expect = 0.0
Identities = 570/1258 (45%), Positives = 795/1258 (63%), Gaps = 25/1258 (1%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
NVC+++P +C ++DAVP+LCNLL Y+D +L E+ A+C+++IVE S ELLD LC+H
Sbjct: 257 NVCRKLPSECPPTLIDAVPILCNLLQYEDGQLSENVALCMIKIVERVSQSCELLDGLCRH 316
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+++Q + LI+ +S LS+TTY ++ +LV+L++ S ++ ++LYE NI+N L+ IL
Sbjct: 317 GLIQQVIHLINLNSRTTLSQTTYNDLLGVLVKLSSGSTVAFKTLYELNISNTLKDILSAY 376
Query: 363 DLSRRTPPSHCE--DISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSF 536
+LS S C D R+QV LKLLN+L+P D + + + K ++ PK
Sbjct: 377 NLSHGMSSS-CSVVDGQRNQVCEVLKLLNELLPT---GDANAEELSEKVSFLVSNPKKLQ 432
Query: 537 QFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLS 716
+F + +LP L+Q V+SGAN + C+++I +V + DML L++N N+ FLAG+ +
Sbjct: 433 KFGLDVLPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESDMLVELLQNTNISSFLAGVFT 492
Query: 717 RKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQS--QLMDQQSS 890
RKDHHVL+ AL + EII++KL SFVKEGV AI L + Q +
Sbjct: 493 RKDHHVLMLALKITEIILQKLSSIFFKSFVKEGVYFAIDALITPEKYKQLIFPVFTGVHP 552
Query: 891 NLGDTDERTAADLSRCLCYAF-DXXXXXXXXXKMCNLPRHTLLPLAQRVMSTFFXXXXXX 1067
++G ++++ + +RCLCYAF C L + ++ LA + + +F
Sbjct: 553 SIGSC-QKSSRENARCLCYAFLSGCFSSTEETGNCKLDKDSVYSLAYHIRTHYFSEELCD 611
Query: 1068 XXXXXXXX-QKLKACCIALDKNVDSALTNC--AEREDYLSQMIVEAVTALNEGESISTFE 1238
Q L+ +LD ++ +L A+ E+ ++ E ++ L GE ISTFE
Sbjct: 612 TDKGVTDILQNLQTLSGSLDDLLNLSLIKDTPAQDEENFYALLAEIMSKLKCGEPISTFE 671
Query: 1239 FIESGFIRFLAHYLTNGDYLHRALGSD--MSFDYLTVLKRLQTFSFTLLSGHS--GQHFP 1406
FIESG ++ +YLTNG Y+ R G +S + + +R + F+ LLS + P
Sbjct: 672 FIESGIVKSFINYLTNGQYM-RKKGEPHIISRQFSIMERRFEVFARLLLSSAEPPSESLP 730
Query: 1407 LASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLC 1586
+ L++ LQ +LS+LE FPVV S K ++ +P HPC++V FV +GETDLC
Sbjct: 731 VLVLIRKLQTSLSSLENFPVVSSQGLKHRNYFATVPNGRCIPHPCVKVRFVRGDGETDLC 790
Query: 1587 LYD-NVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQ--------SSRKVDSKEQ 1739
Y ++ TVD SS +AIE FLW ++++ S +V++ E+
Sbjct: 791 NYTGDIHTVDPFSSLNAIEGFLWSKVGTKKAEQSFEADSLREHQIKLLSNGSTEVNTDEK 850
Query: 1740 VGSSTTQNNEGDITPKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRV 1919
+ S + + +G + PKL+ L+GKQL+ ++TLYQAILQ + E+ + I K WN+V+ +
Sbjct: 851 LQYSVSCSKKG-MKPKLLLYLEGKQLEPTLTLYQAILQQHMKEN-ETISGTKLWNKVYTL 908
Query: 1920 TLRSAKKLQ--LIYPQISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPA 2093
T +SA + + +SA +TL FS F L LP L + +PA
Sbjct: 909 TYKSAGESEDNSSNELLSASDKAATLK-----------FSSFFHGLDCILPSDLAKESPA 957
Query: 2094 YDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSK 2273
YD+LF+L+ +EG+NR++F + S+ER AFAEG + D++K+ S+PQ EF+NSKLT K
Sbjct: 958 YDVLFLLRSIEGMNRMAFHIMSHERIRAFAEGRINTLDNIKLSFPSVPQNEFVNSKLTEK 1017
Query: 2274 LEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEI 2453
LEQQM D A S MPLWC +L+ +CPFLFSF+AR KYF + F +S Q + ++
Sbjct: 1018 LEQQMRDFSAFSIGGMPLWCKELMDSCPFLFSFDARCKYFRIVAFAMSHRQPYIRSYSDL 1077
Query: 2454 STINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLE 2633
T ND RS S LPRKK VHRN IL SA KMM YA +KVLLE+E+ EVGTGLGPTLE
Sbjct: 1078 RTSNDERSNSGGLPRKKVLVHRNKILDSAVKMMDQYAHQKVLLEVEYDEEVGTGLGPTLE 1137
Query: 2634 FYTLVSHAFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFIIVHS--GLFPRPWSNGMDFS 2807
FYTLV FQK +GMWRGDH + ++S G V S GLFPRPW + +D +
Sbjct: 1138 FYTLVGREFQKFDLGMWRGDHGA---------LISIEGRETVESPFGLFPRPWPSTLD-T 1187
Query: 2808 NGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSSAFYKLMLEKDLDIYDIQSFDPELGR 2987
+ + FS+V+K F LLG++ ARAIQDGR++D+ FS AFYKL+L +++ IYDIQSFDPELG
Sbjct: 1188 HELYFSEVVKKFVLLGKIVARAIQDGRVMDIYFSKAFYKLILGQEVSIYDIQSFDPELGT 1247
Query: 2988 TMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGDSK 3167
++EFQAL R LES E+S + + + NT IE+LCLDF++PGYPDY+ S D
Sbjct: 1248 ALLEFQALVDRGKLLESVCEENSSAKLEFCYHNTNIEDLCLDFTVPGYPDYLLISSQDHT 1307
Query: 3168 MVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQD 3347
MVN NLE+YVSLVVDAT+ SGISRQ+E+FKSGF++VFPI+ LQ+FTAEELERL+CGE D
Sbjct: 1308 MVNTKNLEDYVSLVVDATLNSGISRQIEAFKSGFNQVFPIEHLQVFTAEELERLICGEHD 1367
Query: 3348 TWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAAL 3527
+W + EL ++ FDHGY+++S + LEIIQEFD +RAFL FVTGAPRLP GG A+L
Sbjct: 1368 SWALNELLENVKFDHGYTASSPSIVNLLEIIQEFDNEHQRAFLQFVTGAPRLPSGGFASL 1427
Query: 3528 KPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 3701
PKLTIV KH N D +LPSVMTCANYLKLPPYSSKE M+EKLLYAI EGQG+FHLS
Sbjct: 1428 NPKLTIVRKHSSNLVDSDLPSVMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1485
>gb|PIA45462.1| hypothetical protein AQUCO_01700766v1, partial [Aquilegia coerulea]
Length = 1426
Score = 996 bits (2576), Expect = 0.0
Identities = 565/1243 (45%), Positives = 786/1243 (63%), Gaps = 10/1243 (0%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
N+CK++P + VM+AVP+LCNLL Y+D+KLVE+ A CL+ IV+ HS ++LDE+CK
Sbjct: 229 NICKKLPSEFSPQVMEAVPLLCNLLYYEDQKLVENVATCLIWIVKKASHSPDMLDEVCKQ 288
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+ +Q LI+ + L++ Y +I LL RLA+ S+ + SL E NI+N + ILR+
Sbjct: 289 GLTQQVAHLIASNVGNILNQPIYTDLIGLLSRLASGSITASNSLLEHNISNTMSLILRSY 348
Query: 363 DLSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQF 542
DLS PPS ++ +KLL L+PP++ + V ++II +P L QF
Sbjct: 349 DLSHSKPPSLIHEV--------IKLLYVLLPPLSTNCNGKTEVSNMDRIIAHQPGLLQQF 400
Query: 543 AVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSRK 722
+LP L+Q V SGAN I Y C+SV+ + +T DML L+KNAN+ FLAG+++RK
Sbjct: 401 GADVLPVLVQIVNSGANTHICYGCLSVVNKIIHFSTSDMLVDLLKNANISSFLAGIIARK 460
Query: 723 DHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQSQLMDQQSSNL-- 896
+ H+L+ AL +V+ +M++LP NSF+KEGV + I L + C Q L + + L
Sbjct: 461 ELHLLMLALTIVKNVMQELPDAFFNSFIKEGVVYTIDALITPEECPQFILQTSEGAQLLS 520
Query: 897 GDTDERTAADLSRCLCYAFDXXXXXXXXXKMCNLPRHTLLPLAQRVMSTFFXXXXXXXXX 1076
A D CLCY +D ++C L ++ LA+ + +T+F
Sbjct: 521 RSNQRMPANDSVGCLCYVYDIDQSSEN--EICKLEETSVHTLAKYIRTTYFTTKSGCSEL 578
Query: 1077 XXXXX-QKLKACCIALD--KNVDSALTNCAEREDYLSQMIVEAVTALNEGESISTFEFIE 1247
+KL+ C L +N+ + NC E+E LS + + + L+ E +STFEFIE
Sbjct: 579 KSISLLKKLRNLCAVLSDKENMSVSNDNC-EQEKNLSYTLGQILEELSGEEPVSTFEFIE 637
Query: 1248 SGFIRFLAHYLTNGDYLHRALGSDMSFDYLTVL-KRLQTFSFTLLS--GHSGQHFPLASL 1418
SG +R L YL+NG Y +R + + ++L VL KR + F+ LS H + PL L
Sbjct: 638 SGIVRSLVDYLSNGCYRNRVVDPNELLNHLLVLQKRYEVFAKLCLSPPSHPLKDKPLGIL 697
Query: 1419 VQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLCLY-D 1595
+ LQ+ALS LE FPVVL +K K IP + +T HPCL+V F+ EEG+T LC Y
Sbjct: 698 LGKLQSALSLLENFPVVLHDVSKPKETYVTIPFERTTFHPCLKVRFIKEEGDTSLCDYVS 757
Query: 1596 NVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVDSKEQVGSSTTQNNEGD 1775
+V++++ +S +AIE +LWP +K + + E+ +T +
Sbjct: 758 DVVSIEAFTSINAIERYLWP--------------RVKHEAES--ATEECAVATEDLKSSN 801
Query: 1776 ITPKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTL-RSAKKLQLI 1952
T KL+F LD QLD+++TLYQAILQ K+ + D+ V FW +V+ + + + ++
Sbjct: 802 DTQKLLFYLDKGQLDQTLTLYQAILQLKMETENDLSVGPSFWKQVYEIKYTKDTGQKKIN 861
Query: 1953 YPQISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGL 2132
P + + S+ W ++ + N FSSML +E P ++S+ Y++L +LKILE L
Sbjct: 862 IPDVHHNS-GSSREWSHPQI-YLQNAMCFSSMLFSEPPLSFKKSSTIYEILTLLKILEVL 919
Query: 2133 NRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLEQQMHDHLALST 2312
NR + L +ER ++FAEG ++ D+L V +PQTEF+N KLT KLEQQM D +ST
Sbjct: 920 NRSASHLVYHERISSFAEGCDDSLDNLIVPVCGVPQTEFVNLKLTGKLEQQMQDASVVST 979
Query: 2313 ATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEISTINDRRSLSASL 2492
++P WC+QL+ A PFLF FE R KYF + + S Q N I+DR+S ++
Sbjct: 980 GSIPSWCVQLMEAFPFLFGFETRCKYFQMTLVNSSEVQPDN--------IDDRQSQASGS 1031
Query: 2493 PRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVG 2672
PRKKFKV R+ IL SAA+ M + G+K +LE+E+ EVGTG GPT+EF+TLVS FQKVG
Sbjct: 1032 PRKKFKVCRSRILESAAQAMSSHVGRKAILEVEYPEEVGTGQGPTMEFFTLVSQEFQKVG 1091
Query: 2673 MGMWRGDHTSTAKRLRTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLL 2852
MGMWRGD T+ ++ V SGF++ GLFPRPWS N S+V+K F LL
Sbjct: 1092 MGMWRGDPKLTSM---SNSEVDSSGFVVAPFGLFPRPWS-ASSAPNETQLSEVVKKFVLL 1147
Query: 2853 GQLTARAIQDGRILDLPFSSAFYKLMLEKDLDIYDIQSFDPELGRTMIEFQALAKRKGFL 3032
GQL A+A+QDGR+LDLP S + YKL+LE+DL++YDI SFD +GR ++EFQAL RK L
Sbjct: 1148 GQLVAKALQDGRVLDLPLSCSLYKLILEQDLNLYDIFSFDHGVGRALVEFQALVDRKKVL 1207
Query: 3033 ESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVV 3212
+S S + R D F+NTRIE+LCLDF+LPGYPDY +S+ +MVN+SNL+EYVS +
Sbjct: 1208 DSVS----VKRSDSYFQNTRIEDLCLDFTLPGYPDYKLTSVDGHEMVNMSNLQEYVSSTL 1263
Query: 3213 DATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINFDH 3392
DATV +GI RQ+E+FK GF++V P+K+L+IFTAEEL++LLCGEQ+ W+ EL +HI FDH
Sbjct: 1264 DATVNNGICRQVEAFKLGFNQVLPLKSLKIFTAEELDQLLCGEQNAWNYNELLDHIKFDH 1323
Query: 3393 GYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDA 3572
GY+++S +K LEI+QEF+ QRRAFL FVTGAPRLPPGGLAAL PKLTIVCK C A
Sbjct: 1324 GYTASSPTIIKLLEIMQEFEYNQRRAFLQFVTGAPRLPPGGLAALNPKLTIVCKPCKGGA 1383
Query: 3573 DMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 3701
D +LPSVMTCANYLKLPPYSSKE M+E+LLYAI EGQG+F LS
Sbjct: 1384 DGDLPSVMTCANYLKLPPYSSKEIMKERLLYAITEGQGSFLLS 1426
>gb|PIA45461.1| hypothetical protein AQUCO_01700766v1 [Aquilegia coerulea]
Length = 1221
Score = 996 bits (2576), Expect = 0.0
Identities = 565/1243 (45%), Positives = 786/1243 (63%), Gaps = 10/1243 (0%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
N+CK++P + VM+AVP+LCNLL Y+D+KLVE+ A CL+ IV+ HS ++LDE+CK
Sbjct: 24 NICKKLPSEFSPQVMEAVPLLCNLLYYEDQKLVENVATCLIWIVKKASHSPDMLDEVCKQ 83
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+ +Q LI+ + L++ Y +I LL RLA+ S+ + SL E NI+N + ILR+
Sbjct: 84 GLTQQVAHLIASNVGNILNQPIYTDLIGLLSRLASGSITASNSLLEHNISNTMSLILRSY 143
Query: 363 DLSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQF 542
DLS PPS ++ +KLL L+PP++ + V ++II +P L QF
Sbjct: 144 DLSHSKPPSLIHEV--------IKLLYVLLPPLSTNCNGKTEVSNMDRIIAHQPGLLQQF 195
Query: 543 AVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSRK 722
+LP L+Q V SGAN I Y C+SV+ + +T DML L+KNAN+ FLAG+++RK
Sbjct: 196 GADVLPVLVQIVNSGANTHICYGCLSVVNKIIHFSTSDMLVDLLKNANISSFLAGIIARK 255
Query: 723 DHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQSQLMDQQSSNL-- 896
+ H+L+ AL +V+ +M++LP NSF+KEGV + I L + C Q L + + L
Sbjct: 256 ELHLLMLALTIVKNVMQELPDAFFNSFIKEGVVYTIDALITPEECPQFILQTSEGAQLLS 315
Query: 897 GDTDERTAADLSRCLCYAFDXXXXXXXXXKMCNLPRHTLLPLAQRVMSTFFXXXXXXXXX 1076
A D CLCY +D ++C L ++ LA+ + +T+F
Sbjct: 316 RSNQRMPANDSVGCLCYVYDIDQSSEN--EICKLEETSVHTLAKYIRTTYFTTKSGCSEL 373
Query: 1077 XXXXX-QKLKACCIALD--KNVDSALTNCAEREDYLSQMIVEAVTALNEGESISTFEFIE 1247
+KL+ C L +N+ + NC E+E LS + + + L+ E +STFEFIE
Sbjct: 374 KSISLLKKLRNLCAVLSDKENMSVSNDNC-EQEKNLSYTLGQILEELSGEEPVSTFEFIE 432
Query: 1248 SGFIRFLAHYLTNGDYLHRALGSDMSFDYLTVL-KRLQTFSFTLLS--GHSGQHFPLASL 1418
SG +R L YL+NG Y +R + + ++L VL KR + F+ LS H + PL L
Sbjct: 433 SGIVRSLVDYLSNGCYRNRVVDPNELLNHLLVLQKRYEVFAKLCLSPPSHPLKDKPLGIL 492
Query: 1419 VQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLCLY-D 1595
+ LQ+ALS LE FPVVL +K K IP + +T HPCL+V F+ EEG+T LC Y
Sbjct: 493 LGKLQSALSLLENFPVVLHDVSKPKETYVTIPFERTTFHPCLKVRFIKEEGDTSLCDYVS 552
Query: 1596 NVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVDSKEQVGSSTTQNNEGD 1775
+V++++ +S +AIE +LWP +K + + E+ +T +
Sbjct: 553 DVVSIEAFTSINAIERYLWP--------------RVKHEAES--ATEECAVATEDLKSSN 596
Query: 1776 ITPKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTL-RSAKKLQLI 1952
T KL+F LD QLD+++TLYQAILQ K+ + D+ V FW +V+ + + + ++
Sbjct: 597 DTQKLLFYLDKGQLDQTLTLYQAILQLKMETENDLSVGPSFWKQVYEIKYTKDTGQKKIN 656
Query: 1953 YPQISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFVLKILEGL 2132
P + + S+ W ++ + N FSSML +E P ++S+ Y++L +LKILE L
Sbjct: 657 IPDVHHNS-GSSREWSHPQI-YLQNAMCFSSMLFSEPPLSFKKSSTIYEILTLLKILEVL 714
Query: 2133 NRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLEQQMHDHLALST 2312
NR + L +ER ++FAEG ++ D+L V +PQTEF+N KLT KLEQQM D +ST
Sbjct: 715 NRSASHLVYHERISSFAEGCDDSLDNLIVPVCGVPQTEFVNLKLTGKLEQQMQDASVVST 774
Query: 2313 ATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEISTINDRRSLSASL 2492
++P WC+QL+ A PFLF FE R KYF + + S Q N I+DR+S ++
Sbjct: 775 GSIPSWCVQLMEAFPFLFGFETRCKYFQMTLVNSSEVQPDN--------IDDRQSQASGS 826
Query: 2493 PRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVG 2672
PRKKFKV R+ IL SAA+ M + G+K +LE+E+ EVGTG GPT+EF+TLVS FQKVG
Sbjct: 827 PRKKFKVCRSRILESAAQAMSSHVGRKAILEVEYPEEVGTGQGPTMEFFTLVSQEFQKVG 886
Query: 2673 MGMWRGDHTSTAKRLRTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLL 2852
MGMWRGD T+ ++ V SGF++ GLFPRPWS N S+V+K F LL
Sbjct: 887 MGMWRGDPKLTSM---SNSEVDSSGFVVAPFGLFPRPWS-ASSAPNETQLSEVVKKFVLL 942
Query: 2853 GQLTARAIQDGRILDLPFSSAFYKLMLEKDLDIYDIQSFDPELGRTMIEFQALAKRKGFL 3032
GQL A+A+QDGR+LDLP S + YKL+LE+DL++YDI SFD +GR ++EFQAL RK L
Sbjct: 943 GQLVAKALQDGRVLDLPLSCSLYKLILEQDLNLYDIFSFDHGVGRALVEFQALVDRKKVL 1002
Query: 3033 ESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVV 3212
+S S + R D F+NTRIE+LCLDF+LPGYPDY +S+ +MVN+SNL+EYVS +
Sbjct: 1003 DSVS----VKRSDSYFQNTRIEDLCLDFTLPGYPDYKLTSVDGHEMVNMSNLQEYVSSTL 1058
Query: 3213 DATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQNHINFDH 3392
DATV +GI RQ+E+FK GF++V P+K+L+IFTAEEL++LLCGEQ+ W+ EL +HI FDH
Sbjct: 1059 DATVNNGICRQVEAFKLGFNQVLPLKSLKIFTAEELDQLLCGEQNAWNYNELLDHIKFDH 1118
Query: 3393 GYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDA 3572
GY+++S +K LEI+QEF+ QRRAFL FVTGAPRLPPGGLAAL PKLTIVCK C A
Sbjct: 1119 GYTASSPTIIKLLEIMQEFEYNQRRAFLQFVTGAPRLPPGGLAALNPKLTIVCKPCKGGA 1178
Query: 3573 DMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 3701
D +LPSVMTCANYLKLPPYSSKE M+E+LLYAI EGQG+F LS
Sbjct: 1179 DGDLPSVMTCANYLKLPPYSSKEIMKERLLYAITEGQGSFLLS 1221
>ref|XP_023005685.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita maxima]
Length = 1486
Score = 992 bits (2564), Expect = 0.0
Identities = 566/1257 (45%), Positives = 787/1257 (62%), Gaps = 24/1257 (1%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
NVC+++P +C ++DAVP+LCNLL Y+D +L E+ A+C+++IVE S ELLD LC H
Sbjct: 258 NVCRKLPSECPPTLIDAVPILCNLLQYEDGQLSENVALCMIKIVERVSQSCELLDGLCHH 317
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+++Q + LI+ +S LS+TTY ++ +LV+L++ S ++ ++LYE NI+N L+ IL
Sbjct: 318 GLIQQVIHLINLNSRTTLSQTTYNDLLGVLVKLSSGSTVAFKTLYELNISNTLKDILSAY 377
Query: 363 DLSRRTPPSHCE--DISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSF 536
+LS S C D R+QV LKLLN+L+P D + + + K ++ PK
Sbjct: 378 NLSHGMSSS-CSVVDGQRNQVCEVLKLLNELLPT---GDANAEELSEKVSFLVSNPKKLQ 433
Query: 537 QFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLS 716
+F + +LP L+Q V+SGAN C+++I +V + ML L++N N+ FLAG+ +
Sbjct: 434 KFGLDVLPLLVQVVSSGANLYACCGCLTIIYKFVCLGESGMLVELLQNTNISSFLAGVFT 493
Query: 717 RKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQS--QLMDQQSS 890
RKDHHVL+ AL + EII++KL SFVKEGV AI L + Q +
Sbjct: 494 RKDHHVLMLALKITEIILQKLSSIFFKSFVKEGVYFAIDALITPEKYKQLIFPVFTGVHP 553
Query: 891 NLGDTDERTAADLSRCLCYAF-DXXXXXXXXXKMCNLPRHTLLPLAQRVMSTFFXXXXXX 1067
+ G ++++ + +RCLCYAF C L + ++ LA + + +F
Sbjct: 554 SFGSC-QKSSRENARCLCYAFLSGCFSSTEETGNCKLDKDSVYSLAYHIRTHYFSEELCD 612
Query: 1068 XXXXXXXX-QKLKACCIALDKNVDSALTNC--AEREDYLSQMIVEAVTALNEGESISTFE 1238
Q L+ +LD ++ +L A+ E+ ++ E ++ L GE ISTFE
Sbjct: 613 TDKGVTDILQNLQTLSGSLDDLLNLSLIKDTPAQDEENFYALLAEIMSKLKCGEPISTFE 672
Query: 1239 FIESGFIRFLAHYLTNGDYLHRALGSDM-SFDYLTVLKRLQTFSFTLLSGHS--GQHFPL 1409
FIESG ++ +YLTNG Y+ + + S + + +R + F+ LLS + P+
Sbjct: 673 FIESGIVKSFINYLTNGQYMRKKGEPQIISRQFAIMERRFEVFARLLLSSSEPPSESLPV 732
Query: 1410 ASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLCL 1589
L++ LQ +LS+LE FPVV S K ++ +P HPC++V FV +GETDLC
Sbjct: 733 LVLIRKLQTSLSSLENFPVVSSQGLKHRNYFATVPNGRCIPHPCVKVRFVRGDGETDLCN 792
Query: 1590 YD-NVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQ--------SSRKVDSKEQV 1742
Y ++ TVD SS +AIE FLWP ++++ S +V++ E++
Sbjct: 793 YTGDIHTVDPFSSLNAIEGFLWPKVGTKKAEQSFEADSLREHQIKLLSNGSTEVNTDEKL 852
Query: 1743 GSSTTQNNEGDITPKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVT 1922
S + + +G + PKL+ L+GKQL+ ++TLYQAILQ + E+ + K WN+V+ +T
Sbjct: 853 QYSASCSKKG-MKPKLLLYLEGKQLEPTLTLYQAILQQHMKEN-ETTSGTKLWNQVYTLT 910
Query: 1923 LRSAKKLQ--LIYPQISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAY 2096
+SA + + SA +TL FS F L LP L + +PAY
Sbjct: 911 YKSAGEREDNSSNKLFSASDKAATLQ-----------FSSFFHSLDCILPSDLAKESPAY 959
Query: 2097 DMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKL 2276
D+LF+L+ +EG+NR++F + S+ER AFAEG + D++K+ S+PQ EF+NSKLT KL
Sbjct: 960 DVLFLLRSIEGMNRMAFHIMSHERIRAFAEGRINTLDNIKLSVTSVPQNEFVNSKLTEKL 1019
Query: 2277 EQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEIS 2456
EQQM D A S MPLWC +L+ +CPFLFSF+AR KYF + F +S Q + ++
Sbjct: 1020 EQQMRDFSAFSVGGMPLWCKELMDSCPFLFSFDARCKYFQIVAFAMSHRQPYIRSYSDLR 1079
Query: 2457 TINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEF 2636
T ND RS S LPRKK VHRN IL SA KMM YA +KVLLE+E+ EVGTGLGPTLEF
Sbjct: 1080 TSNDERSNSGGLPRKKVLVHRNKILDSAVKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEF 1139
Query: 2637 YTLVSHAFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFIIVHS--GLFPRPWSNGMDFSN 2810
YTLV FQK +GMWRGDH + ++S G V S GLFPRPW + +D +
Sbjct: 1140 YTLVGREFQKYDLGMWRGDHGA---------LISLEGRETVESPFGLFPRPWPSTLD-TR 1189
Query: 2811 GVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSSAFYKLMLEKDLDIYDIQSFDPELGRT 2990
+ FS+V+K F LLG++ ARAIQDGR++D+ FS AFYKL+L +++ IYDIQSFDPELG
Sbjct: 1190 ELYFSEVVKKFVLLGKIVARAIQDGRVMDIYFSKAFYKLILGQEVSIYDIQSFDPELGTA 1249
Query: 2991 MIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKM 3170
++EFQAL R LES E+S + + + NT IE+LCLDF++PGYPDY+ S D M
Sbjct: 1250 LLEFQALVDRGKLLESVCEENSSAKLEFCYHNTNIEDLCLDFTVPGYPDYLLISSQDHTM 1309
Query: 3171 VNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDT 3350
VN NLE+YVSLVVDAT+ SGISRQ+E+FKSGF++VFPI+ LQ+FT EELERL+CGE D+
Sbjct: 1310 VNTKNLEDYVSLVVDATLNSGISRQIEAFKSGFNQVFPIEHLQVFTEEELERLICGEHDS 1369
Query: 3351 WDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALK 3530
W + EL ++ FDHGY+++S + LEIIQEFD +RAFL FVTGAPRLP GG A+L
Sbjct: 1370 WALNELLENVKFDHGYTASSPSIVNLLEIIQEFDNEHQRAFLQFVTGAPRLPSGGFASLN 1429
Query: 3531 PKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 3701
PKLTIV KH N D +LPSVMTCANYLKLPPYSSKE M+EKLLYAI EGQG+FHLS
Sbjct: 1430 PKLTIVRKHSSNLVDSDLPSVMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1486
>ref|XP_023539461.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita pepo
subsp. pepo]
Length = 1485
Score = 991 bits (2563), Expect = 0.0
Identities = 563/1255 (44%), Positives = 790/1255 (62%), Gaps = 22/1255 (1%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
NVC+++P +C ++DAVP+LCNLL Y+D +L E+ A+C+++IVE S ELLD LC H
Sbjct: 257 NVCRKLPSECPPTLIDAVPILCNLLQYEDGQLSENVALCMIKIVERVSQSCELLDGLCHH 316
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+++Q + LI+ +S LS+TTY ++ +LV+L++ S ++ ++LYE NI+N L+ IL
Sbjct: 317 GLIQQVIHLINLNSRTTLSQTTYNDLLGVLVKLSSGSTVAFKTLYELNISNTLKDILSAY 376
Query: 363 DLSRRTPPSHCE--DISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSF 536
+LS S C D R+QV LKLLN+L+P D + + + K ++ PK
Sbjct: 377 NLSHGMSSS-CSVVDGQRNQVCEVLKLLNELLPT---GDANAEELSEKVYFLVSNPKKLQ 432
Query: 537 QFAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLS 716
+F + +LP L+Q V+SGAN + C+++I +V + ML L++N N+ FLAG+ +
Sbjct: 433 KFGLDVLPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESGMLVELLQNTNISSFLAGVFT 492
Query: 717 RKDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQS--QLMDQQSS 890
RKDHHVL+ AL + EII++KL SFVKEGV AI L + Q +
Sbjct: 493 RKDHHVLMLALKITEIILQKLSSIFFKSFVKEGVYFAIDALITPEKYKQLIFPVFTGVHP 552
Query: 891 NLGDTDERTAADLSRCLCYAF-DXXXXXXXXXKMCNLPRHTLLPLAQRVMSTFFXXXXXX 1067
++G ++++ + +RCLCYAF C L + ++ LA + + +F
Sbjct: 553 SIGSC-QKSSRENARCLCYAFLSGCFSSTEETGNCKLDKDSVYSLAYHIRTHYFSEELCD 611
Query: 1068 XXXXXXXX-QKLKACCIALDKNVDSALTN--CAEREDYLSQMIVEAVTALNEGESISTFE 1238
Q L+ +LD ++ +L A+ E+ ++ E ++ L GE ISTFE
Sbjct: 612 TDKGVTDILQNLQTLAGSLDDLLNLSLIKETPAQDEENCYALLAEIMSKLKCGEPISTFE 671
Query: 1239 FIESGFIRFLAHYLTNGDYLHRALGSDMSFDYLTVL-KRLQTFSFTLLSGHS--GQHFPL 1409
FIESG ++ +YLTNG Y+ + + +++ +R + F+ LLS + P+
Sbjct: 672 FIESGIVKSFINYLTNGQYMRKKGEPQIISRQFSIMERRFEVFARLLLSSAEPPSESLPV 731
Query: 1410 ASLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLCL 1589
L++ LQ +LS+LE FPVV S K ++ +P HPC++V FV +GETDLC
Sbjct: 732 LVLIRKLQTSLSSLENFPVVSSQGLKHRNYFATVPNGRCIPHPCVKVRFVRGDGETDLCN 791
Query: 1590 YD-NVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQ--------SSRKVDSKEQV 1742
Y ++ TVD SS +AIE FLW ++++ S +VD+ E++
Sbjct: 792 YTGDIHTVDPFSSLNAIEGFLWSKVGTKKAEQSFEADSLREHQIKLLSNGSTEVDTDEKL 851
Query: 1743 GSSTTQNNEGDITPKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVT 1922
S + + +G + PKL+ L+GKQL+ ++TLYQAILQ + E + + K WN+V+ +T
Sbjct: 852 QYSVSCSKKG-MKPKLLLYLEGKQLEPTLTLYQAILQQHMKES-ETVSGTKLWNQVYTLT 909
Query: 1923 LRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDM 2102
+SA + S D + L + ++ FS F L LP L + +PAYD+
Sbjct: 910 YKSAGE--------SEDNSSNEL-FSASDKAATLKFSSFFHGLDCILPSDLAKESPAYDV 960
Query: 2103 LFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLEQ 2282
LF+L+ +EG+NR++F + S+ER AFAEG + D++K+ S+PQ EF+NSKLT KLEQ
Sbjct: 961 LFLLRSIEGMNRMAFHIMSHERIRAFAEGRINTLDNIKLSVPSVPQNEFVNSKLTEKLEQ 1020
Query: 2283 QMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEISTI 2462
QM D A S MPLWC +L+ +CPFLFSF+AR KYF + F +S Q + ++ T
Sbjct: 1021 QMRDFSAFSIGGMPLWCKELMDSCPFLFSFDARCKYFRIVAFSMSHRQPYIRSYSDLRTS 1080
Query: 2463 NDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYT 2642
ND RS S LPRKK VHRN IL SA KMM YA +KVLLE+E+ EVGTGLGPTLEFYT
Sbjct: 1081 NDERSNSGGLPRKKVLVHRNKILDSAVKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYT 1140
Query: 2643 LVSHAFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFIIVHS--GLFPRPWSNGMDFSNGV 2816
LV FQK +GMWRGDH + ++S G V S GLFPRPW + +D ++ +
Sbjct: 1141 LVGREFQKYDLGMWRGDHGA---------LISIEGRETVESPFGLFPRPWPSTLD-THEL 1190
Query: 2817 SFSKVLKNFFLLGQLTARAIQDGRILDLPFSSAFYKLMLEKDLDIYDIQSFDPELGRTMI 2996
FS+V+K F LLG++ ARAIQDGR++D+ FS AFYKL+L +++ IYDIQSFDPELG ++
Sbjct: 1191 YFSEVVKKFVLLGKIVARAIQDGRVMDIYFSKAFYKLILGQEVSIYDIQSFDPELGTALL 1250
Query: 2997 EFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMVN 3176
EFQAL R LE E+S + + + NT IE+LCLDF++PGYPDY+ S D MVN
Sbjct: 1251 EFQALVDRGKLLEFVCEENSSAKLEFCYHNTNIEDLCLDFTVPGYPDYLLISSQDHTMVN 1310
Query: 3177 ISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWD 3356
NLE+YVSLVVDAT+ SGISRQ+++FKSGF++VFPI+ LQ+FTAEELERL+CGE D+W
Sbjct: 1311 TKNLEDYVSLVVDATLNSGISRQIDAFKSGFNQVFPIEHLQVFTAEELERLICGEHDSWA 1370
Query: 3357 IIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPK 3536
+ EL ++ FDHGY+++S + LEIIQEFD +RAFL FVTGAPRLP GG A+L PK
Sbjct: 1371 LNELLENVKFDHGYTASSPSIVNLLEIIQEFDNEHQRAFLQFVTGAPRLPSGGFASLNPK 1430
Query: 3537 LTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 3701
LTIV KH N D +LPSVMTCANYLKLPPYSSKE M+EKLLYAI EGQG+FHLS
Sbjct: 1431 LTIVRKHSSNLVDSDLPSVMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1485
>ref|XP_023527254.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita pepo
subsp. pepo]
Length = 1489
Score = 986 bits (2550), Expect = 0.0
Identities = 556/1250 (44%), Positives = 781/1250 (62%), Gaps = 17/1250 (1%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
N+CK++P +C +++AVP+LCNL + D +LVE+ A C+++I E S E+LD LC+H
Sbjct: 257 NICKKLPSECPQTLIEAVPILCNLFQHDDGELVENVARCMIKIAECAHQSCEILDGLCQH 316
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+++Q ++LI+ +S +LS+T Y ++ +LV+L++ S+++ ++LYE +I+N L+ IL
Sbjct: 317 GLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLYELDISNTLKEILSVY 376
Query: 363 DLSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQF 542
+LS D R+QV LKLLN+L+P +D + + K + PK +F
Sbjct: 377 NLSHGMSSCAVVDGQRNQVCEVLKLLNELLPT---EDAKAEQLSEKVSFLASNPKQLQKF 433
Query: 543 AVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSRK 722
+ ILP L+Q V+SGA + C+++I ++ + DML L++NAN+ FL G+ +RK
Sbjct: 434 GLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQNANISSFLVGVFTRK 493
Query: 723 DHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQS--QLMDQQSSNL 896
DHHVL+ AL + EII++KL L SFVKEGV AI L + Q + +
Sbjct: 494 DHHVLMLALKITEIILQKLCSIFLKSFVKEGVYFAIGALITPEKYKQLIFPVFAGVHPSF 553
Query: 897 GDTDERTAADLSRCLCYAFDXXXXXXXXXK-MCNLPRHTLLPLAQRVMSTFFXXXXXXXX 1073
G + T + RCLCYAF C L ++ LA + + +F
Sbjct: 554 GSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDDDSVYSLANHIRNNYFTDELCDTD 612
Query: 1074 XXXXXX-QKLKACCIALDKNVDSALTNC--AEREDYLSQMIVEAVTALNEGESISTFEFI 1244
Q L ALD ++ +L A+ E+ ++ E ++ L GE ISTFEFI
Sbjct: 613 KGVSDILQNLLTFSGALDDLLNLSLIKDTPAQDEEKFYALLAEIMSKLKCGEPISTFEFI 672
Query: 1245 ESGFIRFLAHYLTNGDYLHR-ALGSDMSFDYLTVLKRLQTFSFTLLSG--HSGQHFPLAS 1415
ESG ++ +YLTNG YL + +S + + +R + F+ L S H + P+
Sbjct: 673 ESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLLFSSSDHPSVNLPVRV 732
Query: 1416 LVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLC-LY 1592
L++ LQ +LS+LE FPV+ S K ++ +P HPC++V FV +GETDLC +
Sbjct: 733 LLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKVRFVRGDGETDLCDIT 792
Query: 1593 DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVDSKEQVGSSTTQNNEG 1772
++LTVD SS +AIE FLWP ++++ K+ S V ST + ++G
Sbjct: 793 GDILTVDPFSSLNAIEGFLWPKVSRKKAEQSSEADSLREHQVKLLSNVSVEVSTDEKSQG 852
Query: 1773 DIT-------PKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTLRS 1931
+ PKL+ L+GKQL+ ++TLYQ ILQ I E+ + I K W++V+ + +
Sbjct: 853 SASSSKKGTKPKLLLYLEGKQLEPTLTLYQTILQQHIKEN-EAISGTKVWSQVYTIMYKR 911
Query: 1932 AKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFV 2111
A ++ + C S H + SFF +L +LP L R +P YD+LF+
Sbjct: 912 AG-------EVEDNSCNQFFSASDKGATLHFS-SFFCGILDCDLPSDLARESPVYDVLFL 963
Query: 2112 LKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLEQQMH 2291
L+I+EG+NR++F + S+ER AF+EG + D++K+ S+ Q EF+NSKLT KLEQQM
Sbjct: 964 LRIIEGMNRMAFHIMSHERIRAFSEGRISTLDNIKLSVPSVSQNEFVNSKLTEKLEQQMR 1023
Query: 2292 DHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEISTINDR 2471
D A+S MPLWC +L+ +CPFLFSFEAR KYF + +FG+ + Q + ++ T N
Sbjct: 1024 DFSAVSIGGMPLWCKELMDSCPFLFSFEARRKYFRIVVFGMPQ-QPYVRSYSDLGTSNGV 1082
Query: 2472 RSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVS 2651
RS S LPRKK V R+ IL SAAKMM YA +KVLLE+E+ EVGTGLGPTLEFYTLVS
Sbjct: 1083 RSSSGGLPRKKALVLRDKILLSAAKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVS 1142
Query: 2652 HAFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKV 2831
FQK G+GMWRGDH + S+ + I GLFPRPW + +D + FS+V
Sbjct: 1143 QEFQKYGLGMWRGDHDAFIPG--KSLNIEGRETIESPFGLFPRPWLSTVDIGE-LQFSEV 1199
Query: 2832 LKNFFLLGQLTARAIQDGRILDLPFSSAFYKLMLEKDLDIYDIQSFDPELGRTMIEFQAL 3011
+K F L+GQ+ A+AIQDGR++D+ FS AFYKL+L +++ IYDIQSFDPELG ++EFQAL
Sbjct: 1200 IKKFTLMGQIVAKAIQDGRVMDIYFSKAFYKLILGQEVSIYDIQSFDPELGTVLLEFQAL 1259
Query: 3012 AKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLE 3191
R LES E+S R + + +T IE+LCLDF+LPGYPD +S D+ MVN NLE
Sbjct: 1260 VNRSKLLESVCEENSSSRLEFCYHDTNIEDLCLDFTLPGYPDCRLTSSQDNSMVNTKNLE 1319
Query: 3192 EYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQ 3371
+YVSLV DAT+ SGIS Q+E+FKSGF++VFPI+ LQ+FTAEELERL+CGEQD+W + +L
Sbjct: 1320 DYVSLVADATLYSGISTQIEAFKSGFNQVFPIEHLQVFTAEELERLICGEQDSWALSDLL 1379
Query: 3372 NHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVC 3551
++I FDHGY+++S + LEIIQ+FD Q+RAFL FVTGAPRLP GG A+L PKLTIV
Sbjct: 1380 DNIKFDHGYTASSPSIINLLEIIQDFDNEQQRAFLQFVTGAPRLPSGGFASLNPKLTIVR 1439
Query: 3552 KHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 3701
KH N D +LPSVMTCANYLKLPPYSSKE M+EKLLYAI EGQG+FHLS
Sbjct: 1440 KHSSNMVDSDLPSVMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1489
>ref|XP_022983502.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita maxima]
Length = 1489
Score = 984 bits (2543), Expect = 0.0
Identities = 554/1250 (44%), Positives = 781/1250 (62%), Gaps = 17/1250 (1%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
N+CK++P +C +++AVP+LCNL + D +LVE+ A C+++I E S E+LD LC+H
Sbjct: 257 NICKKLPSECPLTLIEAVPILCNLFQHDDGELVENVARCMIKIAECAHQSCEILDGLCQH 316
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+++Q ++LI+ +S +LS+T Y ++ +LV+L++ S+++ ++LYE +I+N L+ IL
Sbjct: 317 GLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLYELDISNTLKEILSVY 376
Query: 363 DLSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQF 542
+LS D R+QV LKLLN+L+P +D + + K + PK +F
Sbjct: 377 NLSHGMSSCAVVDGQRNQVCEVLKLLNELLPT---EDAKAEQLSEKVSFLAINPKQLQKF 433
Query: 543 AVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSRK 722
+ ILP L+Q V+SGA + C+++I ++ + DML L++NAN+ FL G+ +RK
Sbjct: 434 GLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQNANISSFLVGVFTRK 493
Query: 723 DHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQS--QLMDQQSSNL 896
DHHVL+ AL + EII++KL L SFVKEGV AI L + Q + +
Sbjct: 494 DHHVLMLALKITEIILQKLSSIFLKSFVKEGVYFAIDALITPEKYKQLIFPVFAGVHPSF 553
Query: 897 GDTDERTAADLSRCLCYAFDXXXXXXXXXK-MCNLPRHTLLPLAQRVMSTFFXXXXXXXX 1073
G + T + RCLCYAF C L + ++ LA + + +F
Sbjct: 554 GSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDKDSVYSLANHIRNNYFTDELCDTD 612
Query: 1074 XXXXXX-QKLKACCIALDKNVDSALTNC--AEREDYLSQMIVEAVTALNEGESISTFEFI 1244
Q L ALD ++ +L A+ E+ ++ E ++ L GE ISTFEFI
Sbjct: 613 KGVSDILQNLLTFSGALDDLLNLSLIKDTPAQDEEKFYTLLAEIMSKLKCGEPISTFEFI 672
Query: 1245 ESGFIRFLAHYLTNGDYLHR-ALGSDMSFDYLTVLKRLQTFSFTLLSG--HSGQHFPLAS 1415
ESG ++ +YLTNG YL + +S + + +R + F+ L S H + P+
Sbjct: 673 ESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLLFSSSDHPSVNLPVRV 732
Query: 1416 LVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLC-LY 1592
L++ LQ +LS+LE FPV+ S K ++ +P HPC++V FV +GETDLC +
Sbjct: 733 LLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKVRFVRGDGETDLCDIT 792
Query: 1593 DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVDSKEQVGSSTTQNNEG 1772
++LTVD SS +AIE FLWP ++++ K S V ST + ++G
Sbjct: 793 GDILTVDPFSSLNAIEGFLWPKVTRKKAEQSSEADSLREHQVKPLSNVSVEVSTDEKSQG 852
Query: 1773 DIT-------PKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTLRS 1931
+ PKL+ L+GKQL+ ++TLYQ ILQ I E+ + I K W++V+ + +S
Sbjct: 853 SASSSKKGTKPKLLLYLEGKQLEPTLTLYQTILQQHIKEN-EAISGTKVWSQVYTIMYKS 911
Query: 1932 AKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLFV 2111
A ++ + C S H + SFF +L +LP L + +P YD+LF+
Sbjct: 912 AG-------EVEDNSCNQFFSASDKGATLHFS-SFFCGILDCDLPSDLAKESPVYDVLFL 963
Query: 2112 LKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLEQQMH 2291
L+I+EG+NR++F + S+ER AF+EG + +++K+ S+ Q EF+NSKLT KLEQQM
Sbjct: 964 LRIIEGMNRMAFHIMSHERIRAFSEGRISTLNNIKLSVPSVSQNEFVNSKLTEKLEQQMR 1023
Query: 2292 DHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEISTINDR 2471
D A+S MPLWC +L+ +CPFLFSFEAR KYF + +FG+ + Q + ++ T N
Sbjct: 1024 DFSAVSIGGMPLWCKELMDSCPFLFSFEARRKYFRIVVFGMPQ-QPYVRSYSDLGTSNGV 1082
Query: 2472 RSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLVS 2651
RS S PRKK V RN IL SAAKMM YA +KVLLE+E+ EVGTGLGPTLEFYTLVS
Sbjct: 1083 RSSSGGFPRKKALVLRNQILLSAAKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLVS 1142
Query: 2652 HAFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSKV 2831
FQK G+GMWRGDH + S+ + I GLFPRPW + +D + FS+V
Sbjct: 1143 QEFQKYGLGMWRGDHDAFIPG--KSLNIEGRETIESPFGLFPRPWLSTVDIGE-LQFSEV 1199
Query: 2832 LKNFFLLGQLTARAIQDGRILDLPFSSAFYKLMLEKDLDIYDIQSFDPELGRTMIEFQAL 3011
+K F L+GQ+ A+AIQDGR++D+ FS AFYKL+L +++ IYDIQSFDPELG ++EFQAL
Sbjct: 1200 IKKFTLMGQIVAKAIQDGRVMDIYFSKAFYKLILGQEVSIYDIQSFDPELGTVLLEFQAL 1259
Query: 3012 AKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNLE 3191
R LES E+S + + + +T IE+LCLDF+LPGYPD +S D+ MVN NLE
Sbjct: 1260 VNRSKLLESVCEENSSSKLEFCYHDTNIEDLCLDFTLPGYPDCRLTSSQDNSMVNTKNLE 1319
Query: 3192 EYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIELQ 3371
+YVSLV DAT+ SGIS Q+E+FKSGF++VFPI+ LQ+FTAEELERL+CGEQD+W + +L
Sbjct: 1320 DYVSLVADATLYSGISTQIEAFKSGFNQVFPIEHLQVFTAEELERLICGEQDSWALSDLL 1379
Query: 3372 NHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIVC 3551
++I FDHGY+++S + LEIIQ+FD Q+RAFL FVTGAPRLP GG A+L PKLTIV
Sbjct: 1380 DNIKFDHGYTASSPSIINLLEIIQDFDNEQQRAFLQFVTGAPRLPSGGFASLNPKLTIVR 1439
Query: 3552 KHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 3701
KH N D +LPSVMTCANYLKLPPYSSKE M+EKLLYAI EGQG+FHLS
Sbjct: 1440 KHSSNMVDSDLPSVMTCANYLKLPPYSSKEIMKEKLLYAITEGQGSFHLS 1489
>ref|XP_023539460.1| E3 ubiquitin-protein ligase UPL4-like isoform X2 [Cucurbita pepo
subsp. pepo]
Length = 1500
Score = 983 bits (2541), Expect = 0.0
Identities = 564/1279 (44%), Positives = 786/1279 (61%), Gaps = 46/1279 (3%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
NVC+++P +C ++DAVP+LCNLL Y+D +L E+ A+C+++IVE S ELLD LC H
Sbjct: 247 NVCRKLPSECPPTLIDAVPILCNLLQYEDGQLSENVALCMIKIVERVSQSCELLDGLCHH 306
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+++Q + LI+ +S LS+TTY ++ +LV+L++ S ++ ++LYE NI+N L+ IL
Sbjct: 307 GLIQQVIHLINLNSRTTLSQTTYNDLLGVLVKLSSGSTVAFKTLYELNISNTLKDILSAY 366
Query: 363 DLSRRTPPS-HCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQ 539
+LS S D R+QV LKLLN+L+P D + + + K ++ PK +
Sbjct: 367 NLSHGMSSSCSVVDGQRNQVCEVLKLLNELLP---TGDANAEELSEKVYFLVSNPKKLQK 423
Query: 540 FAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSR 719
F + +LP L+Q V+SGAN + C+++I +V + ML L++N N+ FLAG+ +R
Sbjct: 424 FGLDVLPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESGMLVELLQNTNISSFLAGVFTR 483
Query: 720 KDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQ--SQLMDQQSSN 893
KDHHVL+ AL + EII++KL SFVKEGV AI L + Q + +
Sbjct: 484 KDHHVLMLALKITEIILQKLSSIFFKSFVKEGVYFAIDALITPEKYKQLIFPVFTGVHPS 543
Query: 894 LGDTDERTAADLSRCLCYAF-DXXXXXXXXXKMCNLPRHTLLPLAQRVMSTFF-XXXXXX 1067
+G ++++ + +RCLCYAF C L + ++ LA + + +F
Sbjct: 544 IGSC-QKSSRENARCLCYAFLSGCFSSTEETGNCKLDKDSVYSLAYHIRTHYFSEELCDT 602
Query: 1068 XXXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAVTALNEGESISTFEF 1241
Q L+ +LD N+ A+ E+ ++ E ++ L GE ISTFEF
Sbjct: 603 DKGVTDILQNLQTLAGSLDDLLNLSLIKETPAQDEENCYALLAEIMSKLKCGEPISTFEF 662
Query: 1242 IESGFIRFLAHYLTNGDYLH-RALGSDMSFDYLTVLKRLQTFSFTLLSGHS--GQHFPLA 1412
IESG ++ +YLTNG Y+ + +S + + +R + F+ LLS + P+
Sbjct: 663 IESGIVKSFINYLTNGQYMRKKGEPQIISRQFSIMERRFEVFARLLLSSAEPPSESLPVL 722
Query: 1413 SLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLCLY 1592
L++ LQ +LS+LE FPVV S K ++ +P HPC++V FV +GETDLC Y
Sbjct: 723 VLIRKLQTSLSSLENFPVVSSQGLKHRNYFATVPNGRCIPHPCVKVRFVRGDGETDLCNY 782
Query: 1593 -DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIK--------------------- 1706
++ TVD SS +AIE FLW +++
Sbjct: 783 TGDIHTVDPFSSLNAIEGFLWSKVGTKKAEQSFEADSLREHQIKLLSNVCSGWGVNPKLV 842
Query: 1707 ------------QSSRKVDSKEQVGSSTTQNNEGDITPKLIFLLDGKQLDRSMTLYQAIL 1850
Q S +VD+ E++ S + + +G + PKL+ L+GKQL+ ++TLYQAIL
Sbjct: 843 GSDSMSTDLSETQGSTEVDTDEKLQYSVSCSKKG-MKPKLLLYLEGKQLEPTLTLYQAIL 901
Query: 1851 QDKINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNF 2030
Q + E + + K WN+V+ +T +SA + S D + L + ++ F
Sbjct: 902 QQHMKES-ETVSGTKLWNQVYTLTYKSAGE--------SEDNSSNEL-FSASDKAATLKF 951
Query: 2031 SFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDD 2210
S F L LP L + +PAYD+LF+L+ +EG+NR++F + S+ER AFAEG + D+
Sbjct: 952 SSFFHGLDCILPSDLAKESPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAEGRINTLDN 1011
Query: 2211 LKVIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKY 2390
+K+ S+PQ EF+NSKLT KLEQQM D A S MPLWC +L+ +CPFLFSF+AR KY
Sbjct: 1012 IKLSVPSVPQNEFVNSKLTEKLEQQMRDFSAFSIGGMPLWCKELMDSCPFLFSFDARCKY 1071
Query: 2391 FCLNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGK 2570
F + F +S Q + ++ T ND RS S LPRKK VHRN IL SA KMM YA +
Sbjct: 1072 FRIVAFSMSHRQPYIRSYSDLRTSNDERSNSGGLPRKKVLVHRNKILDSAVKMMDQYAHQ 1131
Query: 2571 KVLLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGF 2750
KVLLE+E+ EVGTGLGPTLEFYTLV FQK +GMWRGDH ++S G
Sbjct: 1132 KVLLEVEYDEEVGTGLGPTLEFYTLVGREFQKYDLGMWRGDH---------GALISIEGR 1182
Query: 2751 IIVHS--GLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSSAFYK 2924
V S GLFPRPW + +D ++ + FS+V+K F LLG++ ARAIQDGR++D+ FS AFYK
Sbjct: 1183 ETVESPFGLFPRPWPSTLD-THELYFSEVVKKFVLLGKIVARAIQDGRVMDIYFSKAFYK 1241
Query: 2925 LMLEKDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEEL 3104
L+L +++ IYDIQSFDPELG ++EFQAL R LE E+S + + + NT IE+L
Sbjct: 1242 LILGQEVSIYDIQSFDPELGTALLEFQALVDRGKLLEFVCEENSSAKLEFCYHNTNIEDL 1301
Query: 3105 CLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFP 3284
CLDF++PGYPDY+ S D MVN NLE+YVSLVVDAT+ SGISRQ+++FKSGF++VFP
Sbjct: 1302 CLDFTVPGYPDYLLISSQDHTMVNTKNLEDYVSLVVDATLNSGISRQIDAFKSGFNQVFP 1361
Query: 3285 IKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQR 3464
I+ LQ+FTAEELERL+CGE D+W + EL ++ FDHGY+++S + LEIIQEFD +
Sbjct: 1362 IEHLQVFTAEELERLICGEHDSWALNELLENVKFDHGYTASSPSIVNLLEIIQEFDNEHQ 1421
Query: 3465 RAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKER 3644
RAFL FVTGAPRLP GG A+L PKLTIV KH N D +LPSVMTCANYLKLPPYSSKE
Sbjct: 1422 RAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDSDLPSVMTCANYLKLPPYSSKEI 1481
Query: 3645 MREKLLYAIREGQGAFHLS 3701
M+EKLLYAI EGQG+FHLS
Sbjct: 1482 MKEKLLYAITEGQGSFHLS 1500
>ref|XP_023539462.1| E3 ubiquitin-protein ligase UPL4-like isoform X4 [Cucurbita pepo
subsp. pepo]
Length = 1475
Score = 983 bits (2541), Expect = 0.0
Identities = 564/1279 (44%), Positives = 786/1279 (61%), Gaps = 46/1279 (3%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
NVC+++P +C ++DAVP+LCNLL Y+D +L E+ A+C+++IVE S ELLD LC H
Sbjct: 222 NVCRKLPSECPPTLIDAVPILCNLLQYEDGQLSENVALCMIKIVERVSQSCELLDGLCHH 281
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+++Q + LI+ +S LS+TTY ++ +LV+L++ S ++ ++LYE NI+N L+ IL
Sbjct: 282 GLIQQVIHLINLNSRTTLSQTTYNDLLGVLVKLSSGSTVAFKTLYELNISNTLKDILSAY 341
Query: 363 DLSRRTPPS-HCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQ 539
+LS S D R+QV LKLLN+L+P D + + + K ++ PK +
Sbjct: 342 NLSHGMSSSCSVVDGQRNQVCEVLKLLNELLP---TGDANAEELSEKVYFLVSNPKKLQK 398
Query: 540 FAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSR 719
F + +LP L+Q V+SGAN + C+++I +V + ML L++N N+ FLAG+ +R
Sbjct: 399 FGLDVLPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESGMLVELLQNTNISSFLAGVFTR 458
Query: 720 KDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQ--SQLMDQQSSN 893
KDHHVL+ AL + EII++KL SFVKEGV AI L + Q + +
Sbjct: 459 KDHHVLMLALKITEIILQKLSSIFFKSFVKEGVYFAIDALITPEKYKQLIFPVFTGVHPS 518
Query: 894 LGDTDERTAADLSRCLCYAF-DXXXXXXXXXKMCNLPRHTLLPLAQRVMSTFF-XXXXXX 1067
+G ++++ + +RCLCYAF C L + ++ LA + + +F
Sbjct: 519 IGSC-QKSSRENARCLCYAFLSGCFSSTEETGNCKLDKDSVYSLAYHIRTHYFSEELCDT 577
Query: 1068 XXXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAVTALNEGESISTFEF 1241
Q L+ +LD N+ A+ E+ ++ E ++ L GE ISTFEF
Sbjct: 578 DKGVTDILQNLQTLAGSLDDLLNLSLIKETPAQDEENCYALLAEIMSKLKCGEPISTFEF 637
Query: 1242 IESGFIRFLAHYLTNGDYLH-RALGSDMSFDYLTVLKRLQTFSFTLLSGHS--GQHFPLA 1412
IESG ++ +YLTNG Y+ + +S + + +R + F+ LLS + P+
Sbjct: 638 IESGIVKSFINYLTNGQYMRKKGEPQIISRQFSIMERRFEVFARLLLSSAEPPSESLPVL 697
Query: 1413 SLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLCLY 1592
L++ LQ +LS+LE FPVV S K ++ +P HPC++V FV +GETDLC Y
Sbjct: 698 VLIRKLQTSLSSLENFPVVSSQGLKHRNYFATVPNGRCIPHPCVKVRFVRGDGETDLCNY 757
Query: 1593 -DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIK--------------------- 1706
++ TVD SS +AIE FLW +++
Sbjct: 758 TGDIHTVDPFSSLNAIEGFLWSKVGTKKAEQSFEADSLREHQIKLLSNVCSGWGVNPKLV 817
Query: 1707 ------------QSSRKVDSKEQVGSSTTQNNEGDITPKLIFLLDGKQLDRSMTLYQAIL 1850
Q S +VD+ E++ S + + +G + PKL+ L+GKQL+ ++TLYQAIL
Sbjct: 818 GSDSMSTDLSETQGSTEVDTDEKLQYSVSCSKKG-MKPKLLLYLEGKQLEPTLTLYQAIL 876
Query: 1851 QDKINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNF 2030
Q + E + + K WN+V+ +T +SA + S D + L + ++ F
Sbjct: 877 QQHMKES-ETVSGTKLWNQVYTLTYKSAGE--------SEDNSSNEL-FSASDKAATLKF 926
Query: 2031 SFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDD 2210
S F L LP L + +PAYD+LF+L+ +EG+NR++F + S+ER AFAEG + D+
Sbjct: 927 SSFFHGLDCILPSDLAKESPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAEGRINTLDN 986
Query: 2211 LKVIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKY 2390
+K+ S+PQ EF+NSKLT KLEQQM D A S MPLWC +L+ +CPFLFSF+AR KY
Sbjct: 987 IKLSVPSVPQNEFVNSKLTEKLEQQMRDFSAFSIGGMPLWCKELMDSCPFLFSFDARCKY 1046
Query: 2391 FCLNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGK 2570
F + F +S Q + ++ T ND RS S LPRKK VHRN IL SA KMM YA +
Sbjct: 1047 FRIVAFSMSHRQPYIRSYSDLRTSNDERSNSGGLPRKKVLVHRNKILDSAVKMMDQYAHQ 1106
Query: 2571 KVLLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGF 2750
KVLLE+E+ EVGTGLGPTLEFYTLV FQK +GMWRGDH ++S G
Sbjct: 1107 KVLLEVEYDEEVGTGLGPTLEFYTLVGREFQKYDLGMWRGDH---------GALISIEGR 1157
Query: 2751 IIVHS--GLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSSAFYK 2924
V S GLFPRPW + +D ++ + FS+V+K F LLG++ ARAIQDGR++D+ FS AFYK
Sbjct: 1158 ETVESPFGLFPRPWPSTLD-THELYFSEVVKKFVLLGKIVARAIQDGRVMDIYFSKAFYK 1216
Query: 2925 LMLEKDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEEL 3104
L+L +++ IYDIQSFDPELG ++EFQAL R LE E+S + + + NT IE+L
Sbjct: 1217 LILGQEVSIYDIQSFDPELGTALLEFQALVDRGKLLEFVCEENSSAKLEFCYHNTNIEDL 1276
Query: 3105 CLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFP 3284
CLDF++PGYPDY+ S D MVN NLE+YVSLVVDAT+ SGISRQ+++FKSGF++VFP
Sbjct: 1277 CLDFTVPGYPDYLLISSQDHTMVNTKNLEDYVSLVVDATLNSGISRQIDAFKSGFNQVFP 1336
Query: 3285 IKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQR 3464
I+ LQ+FTAEELERL+CGE D+W + EL ++ FDHGY+++S + LEIIQEFD +
Sbjct: 1337 IEHLQVFTAEELERLICGEHDSWALNELLENVKFDHGYTASSPSIVNLLEIIQEFDNEHQ 1396
Query: 3465 RAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKER 3644
RAFL FVTGAPRLP GG A+L PKLTIV KH N D +LPSVMTCANYLKLPPYSSKE
Sbjct: 1397 RAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDSDLPSVMTCANYLKLPPYSSKEI 1456
Query: 3645 MREKLLYAIREGQGAFHLS 3701
M+EKLLYAI EGQG+FHLS
Sbjct: 1457 MKEKLLYAITEGQGSFHLS 1475
>ref|XP_023539459.1| E3 ubiquitin-protein ligase UPL4-like isoform X1 [Cucurbita pepo
subsp. pepo]
Length = 1510
Score = 983 bits (2541), Expect = 0.0
Identities = 564/1279 (44%), Positives = 786/1279 (61%), Gaps = 46/1279 (3%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
NVC+++P +C ++DAVP+LCNLL Y+D +L E+ A+C+++IVE S ELLD LC H
Sbjct: 257 NVCRKLPSECPPTLIDAVPILCNLLQYEDGQLSENVALCMIKIVERVSQSCELLDGLCHH 316
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+++Q + LI+ +S LS+TTY ++ +LV+L++ S ++ ++LYE NI+N L+ IL
Sbjct: 317 GLIQQVIHLINLNSRTTLSQTTYNDLLGVLVKLSSGSTVAFKTLYELNISNTLKDILSAY 376
Query: 363 DLSRRTPPS-HCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQ 539
+LS S D R+QV LKLLN+L+P D + + + K ++ PK +
Sbjct: 377 NLSHGMSSSCSVVDGQRNQVCEVLKLLNELLP---TGDANAEELSEKVYFLVSNPKKLQK 433
Query: 540 FAVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSR 719
F + +LP L+Q V+SGAN + C+++I +V + ML L++N N+ FLAG+ +R
Sbjct: 434 FGLDVLPLLVQVVSSGANLYVCCGCLTIIYKFVCLGESGMLVELLQNTNISSFLAGVFTR 493
Query: 720 KDHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQ--SQLMDQQSSN 893
KDHHVL+ AL + EII++KL SFVKEGV AI L + Q + +
Sbjct: 494 KDHHVLMLALKITEIILQKLSSIFFKSFVKEGVYFAIDALITPEKYKQLIFPVFTGVHPS 553
Query: 894 LGDTDERTAADLSRCLCYAF-DXXXXXXXXXKMCNLPRHTLLPLAQRVMSTFF-XXXXXX 1067
+G ++++ + +RCLCYAF C L + ++ LA + + +F
Sbjct: 554 IGSC-QKSSRENARCLCYAFLSGCFSSTEETGNCKLDKDSVYSLAYHIRTHYFSEELCDT 612
Query: 1068 XXXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAVTALNEGESISTFEF 1241
Q L+ +LD N+ A+ E+ ++ E ++ L GE ISTFEF
Sbjct: 613 DKGVTDILQNLQTLAGSLDDLLNLSLIKETPAQDEENCYALLAEIMSKLKCGEPISTFEF 672
Query: 1242 IESGFIRFLAHYLTNGDYLH-RALGSDMSFDYLTVLKRLQTFSFTLLSGHS--GQHFPLA 1412
IESG ++ +YLTNG Y+ + +S + + +R + F+ LLS + P+
Sbjct: 673 IESGIVKSFINYLTNGQYMRKKGEPQIISRQFSIMERRFEVFARLLLSSAEPPSESLPVL 732
Query: 1413 SLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLCLY 1592
L++ LQ +LS+LE FPVV S K ++ +P HPC++V FV +GETDLC Y
Sbjct: 733 VLIRKLQTSLSSLENFPVVSSQGLKHRNYFATVPNGRCIPHPCVKVRFVRGDGETDLCNY 792
Query: 1593 -DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIK--------------------- 1706
++ TVD SS +AIE FLW +++
Sbjct: 793 TGDIHTVDPFSSLNAIEGFLWSKVGTKKAEQSFEADSLREHQIKLLSNVCSGWGVNPKLV 852
Query: 1707 ------------QSSRKVDSKEQVGSSTTQNNEGDITPKLIFLLDGKQLDRSMTLYQAIL 1850
Q S +VD+ E++ S + + +G + PKL+ L+GKQL+ ++TLYQAIL
Sbjct: 853 GSDSMSTDLSETQGSTEVDTDEKLQYSVSCSKKG-MKPKLLLYLEGKQLEPTLTLYQAIL 911
Query: 1851 QDKINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNF 2030
Q + E + + K WN+V+ +T +SA + S D + L + ++ F
Sbjct: 912 QQHMKES-ETVSGTKLWNQVYTLTYKSAGE--------SEDNSSNEL-FSASDKAATLKF 961
Query: 2031 SFFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDD 2210
S F L LP L + +PAYD+LF+L+ +EG+NR++F + S+ER AFAEG + D+
Sbjct: 962 SSFFHGLDCILPSDLAKESPAYDVLFLLRSIEGMNRMAFHIMSHERIRAFAEGRINTLDN 1021
Query: 2211 LKVIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKY 2390
+K+ S+PQ EF+NSKLT KLEQQM D A S MPLWC +L+ +CPFLFSF+AR KY
Sbjct: 1022 IKLSVPSVPQNEFVNSKLTEKLEQQMRDFSAFSIGGMPLWCKELMDSCPFLFSFDARCKY 1081
Query: 2391 FCLNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGK 2570
F + F +S Q + ++ T ND RS S LPRKK VHRN IL SA KMM YA +
Sbjct: 1082 FRIVAFSMSHRQPYIRSYSDLRTSNDERSNSGGLPRKKVLVHRNKILDSAVKMMDQYAHQ 1141
Query: 2571 KVLLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGF 2750
KVLLE+E+ EVGTGLGPTLEFYTLV FQK +GMWRGDH ++S G
Sbjct: 1142 KVLLEVEYDEEVGTGLGPTLEFYTLVGREFQKYDLGMWRGDH---------GALISIEGR 1192
Query: 2751 IIVHS--GLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSSAFYK 2924
V S GLFPRPW + +D ++ + FS+V+K F LLG++ ARAIQDGR++D+ FS AFYK
Sbjct: 1193 ETVESPFGLFPRPWPSTLD-THELYFSEVVKKFVLLGKIVARAIQDGRVMDIYFSKAFYK 1251
Query: 2925 LMLEKDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEEL 3104
L+L +++ IYDIQSFDPELG ++EFQAL R LE E+S + + + NT IE+L
Sbjct: 1252 LILGQEVSIYDIQSFDPELGTALLEFQALVDRGKLLEFVCEENSSAKLEFCYHNTNIEDL 1311
Query: 3105 CLDFSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFP 3284
CLDF++PGYPDY+ S D MVN NLE+YVSLVVDAT+ SGISRQ+++FKSGF++VFP
Sbjct: 1312 CLDFTVPGYPDYLLISSQDHTMVNTKNLEDYVSLVVDATLNSGISRQIDAFKSGFNQVFP 1371
Query: 3285 IKALQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQR 3464
I+ LQ+FTAEELERL+CGE D+W + EL ++ FDHGY+++S + LEIIQEFD +
Sbjct: 1372 IEHLQVFTAEELERLICGEHDSWALNELLENVKFDHGYTASSPSIVNLLEIIQEFDNEHQ 1431
Query: 3465 RAFLLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKER 3644
RAFL FVTGAPRLP GG A+L PKLTIV KH N D +LPSVMTCANYLKLPPYSSKE
Sbjct: 1432 RAFLQFVTGAPRLPSGGFASLNPKLTIVRKHSSNLVDSDLPSVMTCANYLKLPPYSSKEI 1491
Query: 3645 MREKLLYAIREGQGAFHLS 3701
M+EKLLYAI EGQG+FHLS
Sbjct: 1492 MKEKLLYAITEGQGSFHLS 1510
>ref|XP_022934795.1| E3 ubiquitin-protein ligase UPL4-like isoform X3 [Cucurbita moschata]
Length = 1489
Score = 979 bits (2530), Expect = 0.0
Identities = 552/1251 (44%), Positives = 780/1251 (62%), Gaps = 18/1251 (1%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
N+CK++P +C +++AVP+LCNL + D +LVE+ A C+++I E S E+LD LC+H
Sbjct: 257 NICKKLPSECPQTLIEAVPILCNLFQHDDGELVENVARCMIKIAECAHQSCEILDGLCQH 316
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+++Q ++LI+ +S +LS+T Y ++ +LV+L++ S+++ ++LYE +I+N L+ IL
Sbjct: 317 GLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLYELDISNTLKEILSVY 376
Query: 363 DLSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQF 542
+LS D R+QV LKLLN+L+P +D + + K + PK +F
Sbjct: 377 NLSHGMSSCAVVDGQRNQVCEVLKLLNELLPT---EDAKAEQLSEKVSFLASNPKQLQKF 433
Query: 543 AVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSRK 722
+ ILP L+Q V+SGA + C+++I ++ + DML L++NAN+ FL G+ +RK
Sbjct: 434 GLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQNANISSFLVGVFTRK 493
Query: 723 DHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQSQLMDQQSS---N 893
DHHVL+ AL + EII++KL L SFVKEGV AI L + Y+ + +S +
Sbjct: 494 DHHVLMLALKITEIILQKLSSIFLKSFVKEGVYFAIDALITPEK-YKQLIFPVFASVHPS 552
Query: 894 LGDTDERTAADLSRCLCYAFDXXXXXXXXXK-MCNLPRHTLLPLAQRVMSTFFXXXXXXX 1070
G + T + RCLCYAF C L ++ LA + + +F
Sbjct: 553 FGSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDDDSVYSLANHIRNNYFTDELCDT 611
Query: 1071 XXXXXXX-QKLKACCIALDKNVDSALTNCA--EREDYLSQMIVEAVTALNEGESISTFEF 1241
Q L ALD ++ +L + E+ ++ E ++ L GE ISTFEF
Sbjct: 612 DKGVSDILQNLLTFSGALDDLLNLSLIKDTPGQDEEKFYALLAEIMSKLKCGEPISTFEF 671
Query: 1242 IESGFIRFLAHYLTNGDYLHR-ALGSDMSFDYLTVLKRLQTFSFTLLSG--HSGQHFPLA 1412
IESG ++ +YLTNG YL + +S + + +R + F+ L S H + P+
Sbjct: 672 IESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLLFSSSDHPSVNLPVR 731
Query: 1413 SLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLC-L 1589
L++ LQ +LS+LE FPV+ S K ++ +P HPC++V FV +GETDLC +
Sbjct: 732 VLLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKVRFVRGDGETDLCDI 791
Query: 1590 YDNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVDSKEQVGSSTTQNNE 1769
++LTVD SS +AIE FLWP ++++ K+ S V ST + +
Sbjct: 792 TGDILTVDPFSSLNAIEGFLWPKVSRKKAEQSSEADSLREHQVKLLSNASVEVSTDEKSL 851
Query: 1770 GDIT-------PKLIFLLDGKQLDRSMTLYQAILQDKINEDPDIIVSKKFWNEVHRVTLR 1928
G + PKL+ L+GKQL+ ++TLYQ ILQ I E+ + I K W++V+ + +
Sbjct: 852 GSASSSKKGTKPKLLLYLEGKQLEPTLTLYQTILQRHIKEN-EAISGTKVWSQVYTIMYK 910
Query: 1929 SAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSFFSSMLLTELPFKLERSNPAYDMLF 2108
A ++ + C S H + SFF +L +LP L + +P YD+LF
Sbjct: 911 RAG-------EVEDNSCNQFFSGSDKGATLHFS-SFFCGILDCDLPSDLAKESPVYDVLF 962
Query: 2109 VLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLKVIAQSLPQTEFINSKLTSKLEQQM 2288
+L+I+EG+NR++F + S+ER AF+EG + D++K+ S+ Q EF+NSKLT KLEQQM
Sbjct: 963 LLRIIEGMNRMAFHIMSHERIRAFSEGRISTLDNIKLSVPSVSQNEFVNSKLTEKLEQQM 1022
Query: 2289 HDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFCLNIFGVSRNQTHNSEKKEISTIND 2468
D A+S MPLWC +L+ +CPFLFSFEAR KYF + +FG+ + Q + ++ T N
Sbjct: 1023 RDFSAVSIGGMPLWCKELMDSCPFLFSFEARRKYFRIVVFGMPQ-QPYVRSYSDLGTSNG 1081
Query: 2469 RRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKVLLEIEFSNEVGTGLGPTLEFYTLV 2648
RS S LPRKK V R+ IL SAAKMM YA +KVLLE+E+ EVGTGLGPTLEFYTLV
Sbjct: 1082 VRSSSGGLPRKKALVLRDKILLSAAKMMDQYAHQKVLLEVEYDEEVGTGLGPTLEFYTLV 1141
Query: 2649 SHAFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFIIVHSGLFPRPWSNGMDFSNGVSFSK 2828
S FQK G+GMWRGDH + S+ + I GLFPRPW + +D + FS+
Sbjct: 1142 SQEFQKYGLGMWRGDHDAFIPG--KSLNIEGRETIESPFGLFPRPWLSTVDIGE-LQFSE 1198
Query: 2829 VLKNFFLLGQLTARAIQDGRILDLPFSSAFYKLMLEKDLDIYDIQSFDPELGRTMIEFQA 3008
V+K F L+GQ+ A+AIQDGR++D+ FS AFYKL+L +++ IYDIQSFDPELG ++EFQA
Sbjct: 1199 VIKRFTLMGQIVAKAIQDGRVMDIYFSKAFYKLILGQEVSIYDIQSFDPELGTVLLEFQA 1258
Query: 3009 LAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDFSLPGYPDYVFSSIGDSKMVNISNL 3188
L R LES E+S + + + +T IE+LCLDF+LPGYPD +S D+ MVN NL
Sbjct: 1259 LVNRSKLLESVCEENSSSKLEFCYHDTNIEDLCLDFTLPGYPDCRLTSSQDNSMVNTKNL 1318
Query: 3189 EEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKALQIFTAEELERLLCGEQDTWDIIEL 3368
E+YVSLV DAT+ SGIS Q+E+FKSGF++VFPI+ LQ+FTAEELERL+CGEQD+W + +L
Sbjct: 1319 EDYVSLVADATLYSGISTQIEAFKSGFNQVFPIEHLQVFTAEELERLICGEQDSWALSDL 1378
Query: 3369 QNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFLLFVTGAPRLPPGGLAALKPKLTIV 3548
++I FDHGY+++S + LEIIQ+FD Q+RAFL FVTGAPRLP GG +L PKLTIV
Sbjct: 1379 LDNIKFDHGYTASSPSIINLLEIIQDFDNEQQRAFLQFVTGAPRLPSGGFTSLNPKLTIV 1438
Query: 3549 CKHCDNDADMELPSVMTCANYLKLPPYSSKERMREKLLYAIREGQGAFHLS 3701
KH N D +LPSVMTCANYLKLPPYSSKE +EKLLYAI EGQG+FHLS
Sbjct: 1439 RKHSSNMVDSDLPSVMTCANYLKLPPYSSKEITKEKLLYAITEGQGSFHLS 1489
>ref|XP_023527253.1| E3 ubiquitin-protein ligase UPL4-like isoform X2 [Cucurbita pepo
subsp. pepo]
Length = 1504
Score = 976 bits (2522), Expect = 0.0
Identities = 559/1275 (43%), Positives = 782/1275 (61%), Gaps = 42/1275 (3%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
N+CK++P +C +++AVP+LCNL + D +LVE+ A C+++I E S E+LD LC+H
Sbjct: 247 NICKKLPSECPQTLIEAVPILCNLFQHDDGELVENVARCMIKIAECAHQSCEILDGLCQH 306
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+++Q ++LI+ +S +LS+T Y ++ +LV+L++ S+++ ++LYE +I+N L+ IL
Sbjct: 307 GLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLYELDISNTLKEILSVY 366
Query: 363 DLSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQF 542
+LS D R+QV LKLLN+L+P +D + + K + PK +F
Sbjct: 367 NLSHGMSSCAVVDGQRNQVCEVLKLLNELLP---TEDAKAEQLSEKVSFLASNPKQLQKF 423
Query: 543 AVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSRK 722
+ ILP L+Q V+SGA + C+++I ++ + DML L++NAN+ FL G+ +RK
Sbjct: 424 GLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQNANISSFLVGVFTRK 483
Query: 723 DHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQ--SQLMDQQSSNL 896
DHHVL+ AL + EII++KL L SFVKEGV AI L + Q + +
Sbjct: 484 DHHVLMLALKITEIILQKLCSIFLKSFVKEGVYFAIGALITPEKYKQLIFPVFAGVHPSF 543
Query: 897 GDTDERTAADLSRCLCYAFDXXXXXXXXXK-MCNLPRHTLLPLAQRVMSTFF-XXXXXXX 1070
G + T + RCLCYAF C L ++ LA + + +F
Sbjct: 544 GSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDDDSVYSLANHIRNNYFTDELCDTD 602
Query: 1071 XXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAVTALNEGESISTFEFI 1244
Q L ALD N+ A+ E+ ++ E ++ L GE ISTFEFI
Sbjct: 603 KGVSDILQNLLTFSGALDDLLNLSLIKDTPAQDEEKFYALLAEIMSKLKCGEPISTFEFI 662
Query: 1245 ESGFIRFLAHYLTNGDYLH-RALGSDMSFDYLTVLKRLQTFSFTLLSG--HSGQHFPLAS 1415
ESG ++ +YLTNG YL + +S + + +R + F+ L S H + P+
Sbjct: 663 ESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLLFSSSDHPSVNLPVRV 722
Query: 1416 LVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLC-LY 1592
L++ LQ +LS+LE FPV+ S K ++ +P HPC++V FV +GETDLC +
Sbjct: 723 LLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKVRFVRGDGETDLCDIT 782
Query: 1593 DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVDS---------KEQVG 1745
++LTVD SS +AIE FLWP ++++ K+ S E VG
Sbjct: 783 GDILTVDPFSSLNAIEGFLWPKVSRKKAEQSSEADSLREHQVKLLSNVCSYFGVNSELVG 842
Query: 1746 S----------------STTQNNEGDIT-------PKLIFLLDGKQLDRSMTLYQAILQD 1856
S ST + ++G + PKL+ L+GKQL+ ++TLYQ ILQ
Sbjct: 843 SNSKSSDLPEIEVSVEVSTDEKSQGSASSSKKGTKPKLLLYLEGKQLEPTLTLYQTILQQ 902
Query: 1857 KINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSF 2036
I E+ + I K W++V+ + + A ++ + C S H + SF
Sbjct: 903 HIKEN-EAISGTKVWSQVYTIMYKRA-------GEVEDNSCNQFFSASDKGATLHFS-SF 953
Query: 2037 FSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLK 2216
F +L +LP L R +P YD+LF+L+I+EG+NR++F + S+ER AF+EG + D++K
Sbjct: 954 FCGILDCDLPSDLARESPVYDVLFLLRIIEGMNRMAFHIMSHERIRAFSEGRISTLDNIK 1013
Query: 2217 VIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFC 2396
+ S+ Q EF+NSKLT KLEQQM D A+S MPLWC +L+ +CPFLFSFEAR KYF
Sbjct: 1014 LSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDSCPFLFSFEARRKYFR 1073
Query: 2397 LNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKV 2576
+ +FG+ + Q + ++ T N RS S LPRKK V R+ IL SAAKMM YA +KV
Sbjct: 1074 IVVFGMPQ-QPYVRSYSDLGTSNGVRSSSGGLPRKKALVLRDKILLSAAKMMDQYAHQKV 1132
Query: 2577 LLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFII 2756
LLE+E+ EVGTGLGPTLEFYTLVS FQK G+GMWRGDH + S+ + I
Sbjct: 1133 LLEVEYDEEVGTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIP--GKSLNIEGRETIE 1190
Query: 2757 VHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSSAFYKLMLE 2936
GLFPRPW + +D + FS+V+K F L+GQ+ A+AIQDGR++D+ FS AFYKL+L
Sbjct: 1191 SPFGLFPRPWLSTVDIGE-LQFSEVIKKFTLMGQIVAKAIQDGRVMDIYFSKAFYKLILG 1249
Query: 2937 KDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDF 3116
+++ IYDIQSFDPELG ++EFQAL R LES E+S R + + +T IE+LCLDF
Sbjct: 1250 QEVSIYDIQSFDPELGTVLLEFQALVNRSKLLESVCEENSSSRLEFCYHDTNIEDLCLDF 1309
Query: 3117 SLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKAL 3296
+LPGYPD +S D+ MVN NLE+YVSLV DAT+ SGIS Q+E+FKSGF++VFPI+ L
Sbjct: 1310 TLPGYPDCRLTSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIEAFKSGFNQVFPIEHL 1369
Query: 3297 QIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFL 3476
Q+FTAEELERL+CGEQD+W + +L ++I FDHGY+++S + LEIIQ+FD Q+RAFL
Sbjct: 1370 QVFTAEELERLICGEQDSWALSDLLDNIKFDHGYTASSPSIINLLEIIQDFDNEQQRAFL 1429
Query: 3477 LFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREK 3656
FVTGAPRLP GG A+L PKLTIV KH N D +LPSVMTCANYLKLPPYSSKE M+EK
Sbjct: 1430 QFVTGAPRLPSGGFASLNPKLTIVRKHSSNMVDSDLPSVMTCANYLKLPPYSSKEIMKEK 1489
Query: 3657 LLYAIREGQGAFHLS 3701
LLYAI EGQG+FHLS
Sbjct: 1490 LLYAITEGQGSFHLS 1504
>ref|XP_023527252.1| E3 ubiquitin-protein ligase UPL4-like isoform X1 [Cucurbita pepo
subsp. pepo]
Length = 1514
Score = 976 bits (2522), Expect = 0.0
Identities = 559/1275 (43%), Positives = 782/1275 (61%), Gaps = 42/1275 (3%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
N+CK++P +C +++AVP+LCNL + D +LVE+ A C+++I E S E+LD LC+H
Sbjct: 257 NICKKLPSECPQTLIEAVPILCNLFQHDDGELVENVARCMIKIAECAHQSCEILDGLCQH 316
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+++Q ++LI+ +S +LS+T Y ++ +LV+L++ S+++ ++LYE +I+N L+ IL
Sbjct: 317 GLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLYELDISNTLKEILSVY 376
Query: 363 DLSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQF 542
+LS D R+QV LKLLN+L+P +D + + K + PK +F
Sbjct: 377 NLSHGMSSCAVVDGQRNQVCEVLKLLNELLP---TEDAKAEQLSEKVSFLASNPKQLQKF 433
Query: 543 AVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSRK 722
+ ILP L+Q V+SGA + C+++I ++ + DML L++NAN+ FL G+ +RK
Sbjct: 434 GLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQNANISSFLVGVFTRK 493
Query: 723 DHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQ--SQLMDQQSSNL 896
DHHVL+ AL + EII++KL L SFVKEGV AI L + Q + +
Sbjct: 494 DHHVLMLALKITEIILQKLCSIFLKSFVKEGVYFAIGALITPEKYKQLIFPVFAGVHPSF 553
Query: 897 GDTDERTAADLSRCLCYAFDXXXXXXXXXK-MCNLPRHTLLPLAQRVMSTFF-XXXXXXX 1070
G + T + RCLCYAF C L ++ LA + + +F
Sbjct: 554 GSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDDDSVYSLANHIRNNYFTDELCDTD 612
Query: 1071 XXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAVTALNEGESISTFEFI 1244
Q L ALD N+ A+ E+ ++ E ++ L GE ISTFEFI
Sbjct: 613 KGVSDILQNLLTFSGALDDLLNLSLIKDTPAQDEEKFYALLAEIMSKLKCGEPISTFEFI 672
Query: 1245 ESGFIRFLAHYLTNGDYLH-RALGSDMSFDYLTVLKRLQTFSFTLLSG--HSGQHFPLAS 1415
ESG ++ +YLTNG YL + +S + + +R + F+ L S H + P+
Sbjct: 673 ESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLLFSSSDHPSVNLPVRV 732
Query: 1416 LVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLC-LY 1592
L++ LQ +LS+LE FPV+ S K ++ +P HPC++V FV +GETDLC +
Sbjct: 733 LLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKVRFVRGDGETDLCDIT 792
Query: 1593 DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVDS---------KEQVG 1745
++LTVD SS +AIE FLWP ++++ K+ S E VG
Sbjct: 793 GDILTVDPFSSLNAIEGFLWPKVSRKKAEQSSEADSLREHQVKLLSNVCSYFGVNSELVG 852
Query: 1746 S----------------STTQNNEGDIT-------PKLIFLLDGKQLDRSMTLYQAILQD 1856
S ST + ++G + PKL+ L+GKQL+ ++TLYQ ILQ
Sbjct: 853 SNSKSSDLPEIEVSVEVSTDEKSQGSASSSKKGTKPKLLLYLEGKQLEPTLTLYQTILQQ 912
Query: 1857 KINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSF 2036
I E+ + I K W++V+ + + A ++ + C S H + SF
Sbjct: 913 HIKEN-EAISGTKVWSQVYTIMYKRA-------GEVEDNSCNQFFSASDKGATLHFS-SF 963
Query: 2037 FSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLK 2216
F +L +LP L R +P YD+LF+L+I+EG+NR++F + S+ER AF+EG + D++K
Sbjct: 964 FCGILDCDLPSDLARESPVYDVLFLLRIIEGMNRMAFHIMSHERIRAFSEGRISTLDNIK 1023
Query: 2217 VIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFC 2396
+ S+ Q EF+NSKLT KLEQQM D A+S MPLWC +L+ +CPFLFSFEAR KYF
Sbjct: 1024 LSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDSCPFLFSFEARRKYFR 1083
Query: 2397 LNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKV 2576
+ +FG+ + Q + ++ T N RS S LPRKK V R+ IL SAAKMM YA +KV
Sbjct: 1084 IVVFGMPQ-QPYVRSYSDLGTSNGVRSSSGGLPRKKALVLRDKILLSAAKMMDQYAHQKV 1142
Query: 2577 LLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFII 2756
LLE+E+ EVGTGLGPTLEFYTLVS FQK G+GMWRGDH + S+ + I
Sbjct: 1143 LLEVEYDEEVGTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIP--GKSLNIEGRETIE 1200
Query: 2757 VHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSSAFYKLMLE 2936
GLFPRPW + +D + FS+V+K F L+GQ+ A+AIQDGR++D+ FS AFYKL+L
Sbjct: 1201 SPFGLFPRPWLSTVDIGE-LQFSEVIKKFTLMGQIVAKAIQDGRVMDIYFSKAFYKLILG 1259
Query: 2937 KDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDF 3116
+++ IYDIQSFDPELG ++EFQAL R LES E+S R + + +T IE+LCLDF
Sbjct: 1260 QEVSIYDIQSFDPELGTVLLEFQALVNRSKLLESVCEENSSSRLEFCYHDTNIEDLCLDF 1319
Query: 3117 SLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKAL 3296
+LPGYPD +S D+ MVN NLE+YVSLV DAT+ SGIS Q+E+FKSGF++VFPI+ L
Sbjct: 1320 TLPGYPDCRLTSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIEAFKSGFNQVFPIEHL 1379
Query: 3297 QIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFL 3476
Q+FTAEELERL+CGEQD+W + +L ++I FDHGY+++S + LEIIQ+FD Q+RAFL
Sbjct: 1380 QVFTAEELERLICGEQDSWALSDLLDNIKFDHGYTASSPSIINLLEIIQDFDNEQQRAFL 1439
Query: 3477 LFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREK 3656
FVTGAPRLP GG A+L PKLTIV KH N D +LPSVMTCANYLKLPPYSSKE M+EK
Sbjct: 1440 QFVTGAPRLPSGGFASLNPKLTIVRKHSSNMVDSDLPSVMTCANYLKLPPYSSKEIMKEK 1499
Query: 3657 LLYAIREGQGAFHLS 3701
LLYAI EGQG+FHLS
Sbjct: 1500 LLYAITEGQGSFHLS 1514
>ref|XP_022983501.1| E3 ubiquitin-protein ligase UPL4-like isoform X2 [Cucurbita maxima]
Length = 1504
Score = 973 bits (2515), Expect = 0.0
Identities = 557/1275 (43%), Positives = 782/1275 (61%), Gaps = 42/1275 (3%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
N+CK++P +C +++AVP+LCNL + D +LVE+ A C+++I E S E+LD LC+H
Sbjct: 247 NICKKLPSECPLTLIEAVPILCNLFQHDDGELVENVARCMIKIAECAHQSCEILDGLCQH 306
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+++Q ++LI+ +S +LS+T Y ++ +LV+L++ S+++ ++LYE +I+N L+ IL
Sbjct: 307 GLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLYELDISNTLKEILSVY 366
Query: 363 DLSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQF 542
+LS D R+QV LKLLN+L+P +D + + K + PK +F
Sbjct: 367 NLSHGMSSCAVVDGQRNQVCEVLKLLNELLP---TEDAKAEQLSEKVSFLAINPKQLQKF 423
Query: 543 AVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSRK 722
+ ILP L+Q V+SGA + C+++I ++ + DML L++NAN+ FL G+ +RK
Sbjct: 424 GLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQNANISSFLVGVFTRK 483
Query: 723 DHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQ--SQLMDQQSSNL 896
DHHVL+ AL + EII++KL L SFVKEGV AI L + Q + +
Sbjct: 484 DHHVLMLALKITEIILQKLSSIFLKSFVKEGVYFAIDALITPEKYKQLIFPVFAGVHPSF 543
Query: 897 GDTDERTAADLSRCLCYAFDXXXXXXXXXK-MCNLPRHTLLPLAQRVMSTFF-XXXXXXX 1070
G + T + RCLCYAF C L + ++ LA + + +F
Sbjct: 544 GSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDKDSVYSLANHIRNNYFTDELCDTD 602
Query: 1071 XXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAVTALNEGESISTFEFI 1244
Q L ALD N+ A+ E+ ++ E ++ L GE ISTFEFI
Sbjct: 603 KGVSDILQNLLTFSGALDDLLNLSLIKDTPAQDEEKFYTLLAEIMSKLKCGEPISTFEFI 662
Query: 1245 ESGFIRFLAHYLTNGDYLH-RALGSDMSFDYLTVLKRLQTFSFTLLSG--HSGQHFPLAS 1415
ESG ++ +YLTNG YL + +S + + +R + F+ L S H + P+
Sbjct: 663 ESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLLFSSSDHPSVNLPVRV 722
Query: 1416 LVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLC-LY 1592
L++ LQ +LS+LE FPV+ S K ++ +P HPC++V FV +GETDLC +
Sbjct: 723 LLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKVRFVRGDGETDLCDIT 782
Query: 1593 DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVDSK---------EQVG 1745
++LTVD SS +AIE FLWP ++++ K S E VG
Sbjct: 783 GDILTVDPFSSLNAIEGFLWPKVTRKKAEQSSEADSLREHQVKPLSNVCSYFGVNPELVG 842
Query: 1746 S----------------STTQNNEGDIT-------PKLIFLLDGKQLDRSMTLYQAILQD 1856
S ST + ++G + PKL+ L+GKQL+ ++TLYQ ILQ
Sbjct: 843 SNSKSSDLPEIEVSVEVSTDEKSQGSASSSKKGTKPKLLLYLEGKQLEPTLTLYQTILQQ 902
Query: 1857 KINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSF 2036
I E+ + I K W++V+ + +SA ++ + C S H + SF
Sbjct: 903 HIKEN-EAISGTKVWSQVYTIMYKSA-------GEVEDNSCNQFFSASDKGATLHFS-SF 953
Query: 2037 FSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLK 2216
F +L +LP L + +P YD+LF+L+I+EG+NR++F + S+ER AF+EG + +++K
Sbjct: 954 FCGILDCDLPSDLAKESPVYDVLFLLRIIEGMNRMAFHIMSHERIRAFSEGRISTLNNIK 1013
Query: 2217 VIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFC 2396
+ S+ Q EF+NSKLT KLEQQM D A+S MPLWC +L+ +CPFLFSFEAR KYF
Sbjct: 1014 LSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDSCPFLFSFEARRKYFR 1073
Query: 2397 LNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKV 2576
+ +FG+ + Q + ++ T N RS S PRKK V RN IL SAAKMM YA +KV
Sbjct: 1074 IVVFGMPQ-QPYVRSYSDLGTSNGVRSSSGGFPRKKALVLRNQILLSAAKMMDQYAHQKV 1132
Query: 2577 LLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFII 2756
LLE+E+ EVGTGLGPTLEFYTLVS FQK G+GMWRGDH + S+ + I
Sbjct: 1133 LLEVEYDEEVGTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIP--GKSLNIEGRETIE 1190
Query: 2757 VHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSSAFYKLMLE 2936
GLFPRPW + +D + FS+V+K F L+GQ+ A+AIQDGR++D+ FS AFYKL+L
Sbjct: 1191 SPFGLFPRPWLSTVDIGE-LQFSEVIKKFTLMGQIVAKAIQDGRVMDIYFSKAFYKLILG 1249
Query: 2937 KDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDF 3116
+++ IYDIQSFDPELG ++EFQAL R LES E+S + + + +T IE+LCLDF
Sbjct: 1250 QEVSIYDIQSFDPELGTVLLEFQALVNRSKLLESVCEENSSSKLEFCYHDTNIEDLCLDF 1309
Query: 3117 SLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKAL 3296
+LPGYPD +S D+ MVN NLE+YVSLV DAT+ SGIS Q+E+FKSGF++VFPI+ L
Sbjct: 1310 TLPGYPDCRLTSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIEAFKSGFNQVFPIEHL 1369
Query: 3297 QIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFL 3476
Q+FTAEELERL+CGEQD+W + +L ++I FDHGY+++S + LEIIQ+FD Q+RAFL
Sbjct: 1370 QVFTAEELERLICGEQDSWALSDLLDNIKFDHGYTASSPSIINLLEIIQDFDNEQQRAFL 1429
Query: 3477 LFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREK 3656
FVTGAPRLP GG A+L PKLTIV KH N D +LPSVMTCANYLKLPPYSSKE M+EK
Sbjct: 1430 QFVTGAPRLPSGGFASLNPKLTIVRKHSSNMVDSDLPSVMTCANYLKLPPYSSKEIMKEK 1489
Query: 3657 LLYAIREGQGAFHLS 3701
LLYAI EGQG+FHLS
Sbjct: 1490 LLYAITEGQGSFHLS 1504
>ref|XP_022983499.1| E3 ubiquitin-protein ligase UPL4-like isoform X1 [Cucurbita maxima]
ref|XP_022983500.1| E3 ubiquitin-protein ligase UPL4-like isoform X1 [Cucurbita maxima]
Length = 1514
Score = 973 bits (2515), Expect = 0.0
Identities = 557/1275 (43%), Positives = 782/1275 (61%), Gaps = 42/1275 (3%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
N+CK++P +C +++AVP+LCNL + D +LVE+ A C+++I E S E+LD LC+H
Sbjct: 257 NICKKLPSECPLTLIEAVPILCNLFQHDDGELVENVARCMIKIAECAHQSCEILDGLCQH 316
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+++Q ++LI+ +S +LS+T Y ++ +LV+L++ S+++ ++LYE +I+N L+ IL
Sbjct: 317 GLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLYELDISNTLKEILSVY 376
Query: 363 DLSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQF 542
+LS D R+QV LKLLN+L+P +D + + K + PK +F
Sbjct: 377 NLSHGMSSCAVVDGQRNQVCEVLKLLNELLP---TEDAKAEQLSEKVSFLAINPKQLQKF 433
Query: 543 AVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSRK 722
+ ILP L+Q V+SGA + C+++I ++ + DML L++NAN+ FL G+ +RK
Sbjct: 434 GLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQNANISSFLVGVFTRK 493
Query: 723 DHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQ--SQLMDQQSSNL 896
DHHVL+ AL + EII++KL L SFVKEGV AI L + Q + +
Sbjct: 494 DHHVLMLALKITEIILQKLSSIFLKSFVKEGVYFAIDALITPEKYKQLIFPVFAGVHPSF 553
Query: 897 GDTDERTAADLSRCLCYAFDXXXXXXXXXK-MCNLPRHTLLPLAQRVMSTFF-XXXXXXX 1070
G + T + RCLCYAF C L + ++ LA + + +F
Sbjct: 554 GSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDKDSVYSLANHIRNNYFTDELCDTD 612
Query: 1071 XXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAVTALNEGESISTFEFI 1244
Q L ALD N+ A+ E+ ++ E ++ L GE ISTFEFI
Sbjct: 613 KGVSDILQNLLTFSGALDDLLNLSLIKDTPAQDEEKFYTLLAEIMSKLKCGEPISTFEFI 672
Query: 1245 ESGFIRFLAHYLTNGDYLH-RALGSDMSFDYLTVLKRLQTFSFTLLSG--HSGQHFPLAS 1415
ESG ++ +YLTNG YL + +S + + +R + F+ L S H + P+
Sbjct: 673 ESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLLFSSSDHPSVNLPVRV 732
Query: 1416 LVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLC-LY 1592
L++ LQ +LS+LE FPV+ S K ++ +P HPC++V FV +GETDLC +
Sbjct: 733 LLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKVRFVRGDGETDLCDIT 792
Query: 1593 DNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVDSK---------EQVG 1745
++LTVD SS +AIE FLWP ++++ K S E VG
Sbjct: 793 GDILTVDPFSSLNAIEGFLWPKVTRKKAEQSSEADSLREHQVKPLSNVCSYFGVNPELVG 852
Query: 1746 S----------------STTQNNEGDIT-------PKLIFLLDGKQLDRSMTLYQAILQD 1856
S ST + ++G + PKL+ L+GKQL+ ++TLYQ ILQ
Sbjct: 853 SNSKSSDLPEIEVSVEVSTDEKSQGSASSSKKGTKPKLLLYLEGKQLEPTLTLYQTILQQ 912
Query: 1857 KINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFSF 2036
I E+ + I K W++V+ + +SA ++ + C S H + SF
Sbjct: 913 HIKEN-EAISGTKVWSQVYTIMYKSA-------GEVEDNSCNQFFSASDKGATLHFS-SF 963
Query: 2037 FSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDLK 2216
F +L +LP L + +P YD+LF+L+I+EG+NR++F + S+ER AF+EG + +++K
Sbjct: 964 FCGILDCDLPSDLAKESPVYDVLFLLRIIEGMNRMAFHIMSHERIRAFSEGRISTLNNIK 1023
Query: 2217 VIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYFC 2396
+ S+ Q EF+NSKLT KLEQQM D A+S MPLWC +L+ +CPFLFSFEAR KYF
Sbjct: 1024 LSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDSCPFLFSFEARRKYFR 1083
Query: 2397 LNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKKV 2576
+ +FG+ + Q + ++ T N RS S PRKK V RN IL SAAKMM YA +KV
Sbjct: 1084 IVVFGMPQ-QPYVRSYSDLGTSNGVRSSSGGFPRKKALVLRNQILLSAAKMMDQYAHQKV 1142
Query: 2577 LLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFII 2756
LLE+E+ EVGTGLGPTLEFYTLVS FQK G+GMWRGDH + S+ + I
Sbjct: 1143 LLEVEYDEEVGTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIP--GKSLNIEGRETIE 1200
Query: 2757 VHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSSAFYKLMLE 2936
GLFPRPW + +D + FS+V+K F L+GQ+ A+AIQDGR++D+ FS AFYKL+L
Sbjct: 1201 SPFGLFPRPWLSTVDIGE-LQFSEVIKKFTLMGQIVAKAIQDGRVMDIYFSKAFYKLILG 1259
Query: 2937 KDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLDF 3116
+++ IYDIQSFDPELG ++EFQAL R LES E+S + + + +T IE+LCLDF
Sbjct: 1260 QEVSIYDIQSFDPELGTVLLEFQALVNRSKLLESVCEENSSSKLEFCYHDTNIEDLCLDF 1319
Query: 3117 SLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKAL 3296
+LPGYPD +S D+ MVN NLE+YVSLV DAT+ SGIS Q+E+FKSGF++VFPI+ L
Sbjct: 1320 TLPGYPDCRLTSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIEAFKSGFNQVFPIEHL 1379
Query: 3297 QIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAFL 3476
Q+FTAEELERL+CGEQD+W + +L ++I FDHGY+++S + LEIIQ+FD Q+RAFL
Sbjct: 1380 QVFTAEELERLICGEQDSWALSDLLDNIKFDHGYTASSPSIINLLEIIQDFDNEQQRAFL 1439
Query: 3477 LFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMREK 3656
FVTGAPRLP GG A+L PKLTIV KH N D +LPSVMTCANYLKLPPYSSKE M+EK
Sbjct: 1440 QFVTGAPRLPSGGFASLNPKLTIVRKHSSNMVDSDLPSVMTCANYLKLPPYSSKEIMKEK 1499
Query: 3657 LLYAIREGQGAFHLS 3701
LLYAI EGQG+FHLS
Sbjct: 1500 LLYAITEGQGSFHLS 1514
>ref|XP_022934794.1| E3 ubiquitin-protein ligase UPL4-like isoform X2 [Cucurbita moschata]
Length = 1504
Score = 967 bits (2501), Expect = 0.0
Identities = 553/1276 (43%), Positives = 781/1276 (61%), Gaps = 43/1276 (3%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
N+CK++P +C +++AVP+LCNL + D +LVE+ A C+++I E S E+LD LC+H
Sbjct: 247 NICKKLPSECPQTLIEAVPILCNLFQHDDGELVENVARCMIKIAECAHQSCEILDGLCQH 306
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+++Q ++LI+ +S +LS+T Y ++ +LV+L++ S+++ ++LYE +I+N L+ IL
Sbjct: 307 GLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLYELDISNTLKEILSVY 366
Query: 363 DLSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQF 542
+LS D R+QV LKLLN+L+P +D + + K + PK +F
Sbjct: 367 NLSHGMSSCAVVDGQRNQVCEVLKLLNELLP---TEDAKAEQLSEKVSFLASNPKQLQKF 423
Query: 543 AVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSRK 722
+ ILP L+Q V+SGA + C+++I ++ + DML L++NAN+ FL G+ +RK
Sbjct: 424 GLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQNANISSFLVGVFTRK 483
Query: 723 DHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQSQLMDQQSS---N 893
DHHVL+ AL + EII++KL L SFVKEGV AI L + Y+ + +S +
Sbjct: 484 DHHVLMLALKITEIILQKLSSIFLKSFVKEGVYFAIDALITPEK-YKQLIFPVFASVHPS 542
Query: 894 LGDTDERTAADLSRCLCYAFDXXXXXXXXXK-MCNLPRHTLLPLAQRVMSTFF-XXXXXX 1067
G + T + RCLCYAF C L ++ LA + + +F
Sbjct: 543 FGSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDDDSVYSLANHIRNNYFTDELCDT 601
Query: 1068 XXXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAVTALNEGESISTFEF 1241
Q L ALD N+ + E+ ++ E ++ L GE ISTFEF
Sbjct: 602 DKGVSDILQNLLTFSGALDDLLNLSLIKDTPGQDEEKFYALLAEIMSKLKCGEPISTFEF 661
Query: 1242 IESGFIRFLAHYLTNGDYLH-RALGSDMSFDYLTVLKRLQTFSFTLLSG--HSGQHFPLA 1412
IESG ++ +YLTNG YL + +S + + +R + F+ L S H + P+
Sbjct: 662 IESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLLFSSSDHPSVNLPVR 721
Query: 1413 SLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLC-L 1589
L++ LQ +LS+LE FPV+ S K ++ +P HPC++V FV +GETDLC +
Sbjct: 722 VLLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKVRFVRGDGETDLCDI 781
Query: 1590 YDNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVDSK---------EQV 1742
++LTVD SS +AIE FLWP ++++ K+ S E V
Sbjct: 782 TGDILTVDPFSSLNAIEGFLWPKVSRKKAEQSSEADSLREHQVKLLSNVCSYSGVNPELV 841
Query: 1743 GSSTTQNNEGDI-----------------------TPKLIFLLDGKQLDRSMTLYQAILQ 1853
GS++ ++ +I PKL+ L+GKQL+ ++TLYQ ILQ
Sbjct: 842 GSNSKSSDLPEIEASVEVSTDEKSLGSASSSKKGTKPKLLLYLEGKQLEPTLTLYQTILQ 901
Query: 1854 DKINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFS 2033
I E+ + I K W++V+ + + A ++ + C S H + S
Sbjct: 902 RHIKEN-EAISGTKVWSQVYTIMYKRA-------GEVEDNSCNQFFSGSDKGATLHFS-S 952
Query: 2034 FFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDL 2213
FF +L +LP L + +P YD+LF+L+I+EG+NR++F + S+ER AF+EG + D++
Sbjct: 953 FFCGILDCDLPSDLAKESPVYDVLFLLRIIEGMNRMAFHIMSHERIRAFSEGRISTLDNI 1012
Query: 2214 KVIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYF 2393
K+ S+ Q EF+NSKLT KLEQQM D A+S MPLWC +L+ +CPFLFSFEAR KYF
Sbjct: 1013 KLSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDSCPFLFSFEARRKYF 1072
Query: 2394 CLNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKK 2573
+ +FG+ + Q + ++ T N RS S LPRKK V R+ IL SAAKMM YA +K
Sbjct: 1073 RIVVFGMPQ-QPYVRSYSDLGTSNGVRSSSGGLPRKKALVLRDKILLSAAKMMDQYAHQK 1131
Query: 2574 VLLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFI 2753
VLLE+E+ EVGTGLGPTLEFYTLVS FQK G+GMWRGDH + S+ + I
Sbjct: 1132 VLLEVEYDEEVGTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIP--GKSLNIEGRETI 1189
Query: 2754 IVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSSAFYKLML 2933
GLFPRPW + +D + FS+V+K F L+GQ+ A+AIQDGR++D+ FS AFYKL+L
Sbjct: 1190 ESPFGLFPRPWLSTVDIGE-LQFSEVIKRFTLMGQIVAKAIQDGRVMDIYFSKAFYKLIL 1248
Query: 2934 EKDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLD 3113
+++ IYDIQSFDPELG ++EFQAL R LES E+S + + + +T IE+LCLD
Sbjct: 1249 GQEVSIYDIQSFDPELGTVLLEFQALVNRSKLLESVCEENSSSKLEFCYHDTNIEDLCLD 1308
Query: 3114 FSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKA 3293
F+LPGYPD +S D+ MVN NLE+YVSLV DAT+ SGIS Q+E+FKSGF++VFPI+
Sbjct: 1309 FTLPGYPDCRLTSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIEAFKSGFNQVFPIEH 1368
Query: 3294 LQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAF 3473
LQ+FTAEELERL+CGEQD+W + +L ++I FDHGY+++S + LEIIQ+FD Q+RAF
Sbjct: 1369 LQVFTAEELERLICGEQDSWALSDLLDNIKFDHGYTASSPSIINLLEIIQDFDNEQQRAF 1428
Query: 3474 LLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMRE 3653
L FVTGAPRLP GG +L PKLTIV KH N D +LPSVMTCANYLKLPPYSSKE +E
Sbjct: 1429 LQFVTGAPRLPSGGFTSLNPKLTIVRKHSSNMVDSDLPSVMTCANYLKLPPYSSKEITKE 1488
Query: 3654 KLLYAIREGQGAFHLS 3701
KLLYAI EGQG+FHLS
Sbjct: 1489 KLLYAITEGQGSFHLS 1504
>ref|XP_022934791.1| E3 ubiquitin-protein ligase UPL4-like isoform X1 [Cucurbita moschata]
ref|XP_022934792.1| E3 ubiquitin-protein ligase UPL4-like isoform X1 [Cucurbita moschata]
Length = 1514
Score = 967 bits (2501), Expect = 0.0
Identities = 553/1276 (43%), Positives = 781/1276 (61%), Gaps = 43/1276 (3%)
Frame = +3
Query: 3 NVCKRVPEDCFSIVMDAVPMLCNLLDYQDRKLVESTAMCLMRIVENFGHSSELLDELCKH 182
N+CK++P +C +++AVP+LCNL + D +LVE+ A C+++I E S E+LD LC+H
Sbjct: 257 NICKKLPSECPQTLIEAVPILCNLFQHDDGELVENVARCMIKIAECAHQSCEILDGLCQH 316
Query: 183 GVVKQSVKLISPHSPIALSETTYIGMIELLVRLANRSLLSIRSLYESNINNILESILRNL 362
G+++Q ++LI+ +S +LS+T Y ++ +LV+L++ S+++ ++LYE +I+N L+ IL
Sbjct: 317 GLIQQVIRLINLNSRTSLSQTIYNDLLGILVKLSSGSIVAFKTLYELDISNTLKEILSVY 376
Query: 363 DLSRRTPPSHCEDISRDQVYVALKLLNQLIPPVARDDQDIQLVLGKEKIIIDEPKLSFQF 542
+LS D R+QV LKLLN+L+P +D + + K + PK +F
Sbjct: 377 NLSHGMSSCAVVDGQRNQVCEVLKLLNELLP---TEDAKAEQLSEKVSFLASNPKQLQKF 433
Query: 543 AVVILPALIQAVTSGANACIHYACVSVIKNYVLIATPDMLRALIKNANMPKFLAGLLSRK 722
+ ILP L+Q V+SGA + C+++I ++ + DML L++NAN+ FL G+ +RK
Sbjct: 434 GLDILPLLVQVVSSGAKLYVCCGCLTIIYKFICLGESDMLVELLQNANISSFLVGVFTRK 493
Query: 723 DHHVLIPALNVVEIIMKKLPGELLNSFVKEGVSHAIRVLSKQDGCYQSQLMDQQSS---N 893
DHHVL+ AL + EII++KL L SFVKEGV AI L + Y+ + +S +
Sbjct: 494 DHHVLMLALKITEIILQKLSSIFLKSFVKEGVYFAIDALITPEK-YKQLIFPVFASVHPS 552
Query: 894 LGDTDERTAADLSRCLCYAFDXXXXXXXXXK-MCNLPRHTLLPLAQRVMSTFF-XXXXXX 1067
G + T + RCLCYAF C L ++ LA + + +F
Sbjct: 553 FGSCQKSTREN-GRCLCYAFSSGCFSSVAETGSCKLDDDSVYSLANHIRNNYFTDELCDT 611
Query: 1068 XXXXXXXXQKLKACCIALDK--NVDSALTNCAEREDYLSQMIVEAVTALNEGESISTFEF 1241
Q L ALD N+ + E+ ++ E ++ L GE ISTFEF
Sbjct: 612 DKGVSDILQNLLTFSGALDDLLNLSLIKDTPGQDEEKFYALLAEIMSKLKCGEPISTFEF 671
Query: 1242 IESGFIRFLAHYLTNGDYLH-RALGSDMSFDYLTVLKRLQTFSFTLLSG--HSGQHFPLA 1412
IESG ++ +YLTNG YL + +S + + +R + F+ L S H + P+
Sbjct: 672 IESGIVKSFINYLTNGQYLRKKGEPQTISRQFSIMERRFEAFARLLFSSSDHPSVNLPVR 731
Query: 1413 SLVQHLQNALSALECFPVVLSAANKSKSRSTDIPAKSSTKHPCLQVHFVAEEGETDLC-L 1589
L++ LQ +LS+LE FPV+ S K ++ +P HPC++V FV +GETDLC +
Sbjct: 732 VLLRKLQISLSSLENFPVISSQGFKHRNYFATVPNGRCIPHPCVKVRFVRGDGETDLCDI 791
Query: 1590 YDNVLTVDISSSYDAIEEFLWPXXXXXXXXXXXXXXNIKQSSRKVDSK---------EQV 1742
++LTVD SS +AIE FLWP ++++ K+ S E V
Sbjct: 792 TGDILTVDPFSSLNAIEGFLWPKVSRKKAEQSSEADSLREHQVKLLSNVCSYSGVNPELV 851
Query: 1743 GSSTTQNNEGDI-----------------------TPKLIFLLDGKQLDRSMTLYQAILQ 1853
GS++ ++ +I PKL+ L+GKQL+ ++TLYQ ILQ
Sbjct: 852 GSNSKSSDLPEIEASVEVSTDEKSLGSASSSKKGTKPKLLLYLEGKQLEPTLTLYQTILQ 911
Query: 1854 DKINEDPDIIVSKKFWNEVHRVTLRSAKKLQLIYPQISADPCQSTLSWDKTELPWHNNFS 2033
I E+ + I K W++V+ + + A ++ + C S H + S
Sbjct: 912 RHIKEN-EAISGTKVWSQVYTIMYKRA-------GEVEDNSCNQFFSGSDKGATLHFS-S 962
Query: 2034 FFSSMLLTELPFKLERSNPAYDMLFVLKILEGLNRVSFQLFSNERSNAFAEGTLENFDDL 2213
FF +L +LP L + +P YD+LF+L+I+EG+NR++F + S+ER AF+EG + D++
Sbjct: 963 FFCGILDCDLPSDLAKESPVYDVLFLLRIIEGMNRMAFHIMSHERIRAFSEGRISTLDNI 1022
Query: 2214 KVIAQSLPQTEFINSKLTSKLEQQMHDHLALSTATMPLWCMQLVVACPFLFSFEARWKYF 2393
K+ S+ Q EF+NSKLT KLEQQM D A+S MPLWC +L+ +CPFLFSFEAR KYF
Sbjct: 1023 KLSVPSVSQNEFVNSKLTEKLEQQMRDFSAVSIGGMPLWCKELMDSCPFLFSFEARRKYF 1082
Query: 2394 CLNIFGVSRNQTHNSEKKEISTINDRRSLSASLPRKKFKVHRNNILASAAKMMQWYAGKK 2573
+ +FG+ + Q + ++ T N RS S LPRKK V R+ IL SAAKMM YA +K
Sbjct: 1083 RIVVFGMPQ-QPYVRSYSDLGTSNGVRSSSGGLPRKKALVLRDKILLSAAKMMDQYAHQK 1141
Query: 2574 VLLEIEFSNEVGTGLGPTLEFYTLVSHAFQKVGMGMWRGDHTSTAKRLRTSMVVSDSGFI 2753
VLLE+E+ EVGTGLGPTLEFYTLVS FQK G+GMWRGDH + S+ + I
Sbjct: 1142 VLLEVEYDEEVGTGLGPTLEFYTLVSQEFQKYGLGMWRGDHDAFIP--GKSLNIEGRETI 1199
Query: 2754 IVHSGLFPRPWSNGMDFSNGVSFSKVLKNFFLLGQLTARAIQDGRILDLPFSSAFYKLML 2933
GLFPRPW + +D + FS+V+K F L+GQ+ A+AIQDGR++D+ FS AFYKL+L
Sbjct: 1200 ESPFGLFPRPWLSTVDIGE-LQFSEVIKRFTLMGQIVAKAIQDGRVMDIYFSKAFYKLIL 1258
Query: 2934 EKDLDIYDIQSFDPELGRTMIEFQALAKRKGFLESKSGEHSLDRFDPIFRNTRIEELCLD 3113
+++ IYDIQSFDPELG ++EFQAL R LES E+S + + + +T IE+LCLD
Sbjct: 1259 GQEVSIYDIQSFDPELGTVLLEFQALVNRSKLLESVCEENSSSKLEFCYHDTNIEDLCLD 1318
Query: 3114 FSLPGYPDYVFSSIGDSKMVNISNLEEYVSLVVDATVRSGISRQLESFKSGFSKVFPIKA 3293
F+LPGYPD +S D+ MVN NLE+YVSLV DAT+ SGIS Q+E+FKSGF++VFPI+
Sbjct: 1319 FTLPGYPDCRLTSSQDNSMVNTKNLEDYVSLVADATLYSGISTQIEAFKSGFNQVFPIEH 1378
Query: 3294 LQIFTAEELERLLCGEQDTWDIIELQNHINFDHGYSSTSRPAMKFLEIIQEFDARQRRAF 3473
LQ+FTAEELERL+CGEQD+W + +L ++I FDHGY+++S + LEIIQ+FD Q+RAF
Sbjct: 1379 LQVFTAEELERLICGEQDSWALSDLLDNIKFDHGYTASSPSIINLLEIIQDFDNEQQRAF 1438
Query: 3474 LLFVTGAPRLPPGGLAALKPKLTIVCKHCDNDADMELPSVMTCANYLKLPPYSSKERMRE 3653
L FVTGAPRLP GG +L PKLTIV KH N D +LPSVMTCANYLKLPPYSSKE +E
Sbjct: 1439 LQFVTGAPRLPSGGFTSLNPKLTIVRKHSSNMVDSDLPSVMTCANYLKLPPYSSKEITKE 1498
Query: 3654 KLLYAIREGQGAFHLS 3701
KLLYAI EGQG+FHLS
Sbjct: 1499 KLLYAITEGQGSFHLS 1514