BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ophiopogon21_contig00033682
         (446 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_010921974.1| PREDICTED: probable RNA-dependent RNA polyme...    66   9e-09
ref|XP_006346784.1| PREDICTED: RNA-dependent RNA polymerase 6-li...    64   3e-08
gb|EEE54718.1| hypothetical protein OsJ_02054 [Oryza sativa Japo...    62   2e-07
ref|XP_010319120.1| PREDICTED: RNA-dependent RNA polymerase 6 is...    61   3e-07
ref|NP_001266205.1| RNA-dependent RNA polymerase 6 [Solanum lyco...    61   3e-07
ref|XP_008778879.1| PREDICTED: LOW QUALITY PROTEIN: probable RNA...    60   5e-07
ref|XP_009609663.1| PREDICTED: RNA-dependent RNA polymerase 6, p...    60   6e-07
emb|CAR47810.1| RNA-dependent RNA polymerase 6 [Nicotiana tabacum]     60   6e-07
gb|ADI52625.1| RNA-dependent RNA polymerase 6 [Nicotiana tabacum]      60   6e-07
ref|XP_002282647.1| PREDICTED: RNA-dependent RNA polymerase 6 [V...    60   6e-07
gb|ACO72600.1| RNA-dependent RNA polymerase 6 [Nicotiana glutinosa]    60   8e-07
ref|XP_009389500.1| PREDICTED: LOW QUALITY PROTEIN: probable RNA...    59   1e-06
sp|Q8LHH9.1|SHL2_ORYSJ RecName: Full=Probable RNA-dependent RNA ...    59   1e-06
gb|ACA13601.1| RNA-dependent RNA polymerase [Nicotiana attenuata]      58   2e-06
gb|AAU21242.1| putative RNA-dependent RNA polymerase SDE1 [Nicot...    58   3e-06
ref|XP_008219491.1| PREDICTED: RNA-dependent RNA polymerase 6 [P...    57   5e-06
ref|XP_002515520.1| RNA-dependent RNA polymerase, putative [Rici...    57   5e-06
ref|XP_007225440.1| hypothetical protein PRUPE_ppa000422mg [Prun...    57   7e-06
dbj|BAF96019.1| RNA-dependent RNA polymerase 6 [Nicotiana tabacum]     57   7e-06

>ref|XP_010921974.1| PREDICTED: probable RNA-dependent RNA polymerase SHL2 [Elaeis
           guineensis]
          Length = 1198

 Score = 66.2 bits (160), Expect = 9e-09
 Identities = 35/96 (36%), Positives = 53/96 (55%), Gaps = 10/96 (10%)
 Frame = -2

Query: 259 PFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLFTGK-------- 104
           PF F D   +IG+L     FL  WK    PK  ++F I PF+  C++LF+ +        
Sbjct: 132 PFRFVDVGVEIGTLASRDEFLVAWK---GPKLGVDFLIDPFDGCCRILFSKETAFAFKDI 188

Query: 103 --FEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
               VI+C  K++FL+R + E+K+  DR  HV++FQ
Sbjct: 189 KEMAVIKCDFKVEFLVRDINEVKLFTDRYPHVMLFQ 224


>ref|XP_006346784.1| PREDICTED: RNA-dependent RNA polymerase 6-like isoform X1 [Solanum
           tuberosum] gi|565360032|ref|XP_006346785.1| PREDICTED:
           RNA-dependent RNA polymerase 6-like isoform X2 [Solanum
           tuberosum]
          Length = 1198

 Score = 64.3 bits (155), Expect = 3e-08
 Identities = 37/99 (37%), Positives = 51/99 (51%), Gaps = 10/99 (10%)
 Frame = -2

Query: 268 SIFPFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLFT------- 110
           +I PF F D   +IG L     F+  W+    P S +NF + PFN +CK+LFT       
Sbjct: 125 TIMPFKFSDVGVEIGVLVSNDDFVVGWR---GPHSGVNFLVDPFNGTCKILFTRDTAFCF 181

Query: 109 ---GKFEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
              GK  VI+C  K++FLLR + E+    D    VL+ Q
Sbjct: 182 KGEGKHAVIKCNFKIEFLLREINEINECKDFASLVLLLQ 220


>gb|EEE54718.1| hypothetical protein OsJ_02054 [Oryza sativa Japonica Group]
          Length = 1571

 Score = 61.6 bits (148), Expect = 2e-07
 Identities = 45/147 (30%), Positives = 67/147 (45%), Gaps = 45/147 (30%)
 Frame = -2

Query: 343 ATHTMALSLEHRVHCIIWRCRVKSS----------------------------------- 269
           A   +A  LEH V  ++WRCRVK+S                                   
Sbjct: 42  AAGDLADFLEHEVG-LVWRCRVKTSWTPPDSYPDFALPTAPASASAAAAPPRSLRVSRRS 100

Query: 268 SIFPFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLFT------- 110
           SI PF FPD   ++G+L  P  FL  W+    P + ++  + PF+  C+L+FT       
Sbjct: 101 SIAPFRFPDVRLEVGALPSPGAFLAAWR---GPDAGLDLSVDPFDGCCRLVFTRDTAFTF 157

Query: 109 -GKFEV--IRCTLKLDFLLRGVMEMKI 38
            G  EV  IRC +KL+F +R V+E+++
Sbjct: 158 PGFREVAAIRCDVKLEFPVRDVLEVRL 184



 Score = 58.5 bits (140), Expect = 2e-06
 Identities = 33/90 (36%), Positives = 52/90 (57%), Gaps = 10/90 (11%)
 Frame = -2

Query: 277 KSSSIFPFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLFT---- 110
           + SSI PF FPD   ++G+L  P  FL  W+    P + ++  + PF+  C+L+FT    
Sbjct: 531 RRSSIAPFRFPDVRLEVGALPWPGAFLAAWR---GPDAGLDLSVDPFDGCCRLVFTRDTA 587

Query: 109 ----GKFEV--IRCTLKLDFLLRGVMEMKI 38
               G  EV  IRC +KL+F +R V+E+++
Sbjct: 588 FTFPGFREVAAIRCDVKLEFPVRDVLEVRL 617


>ref|XP_010319120.1| PREDICTED: RNA-dependent RNA polymerase 6 isoform X1 [Solanum
           lycopersicum] gi|723688503|ref|XP_010319121.1|
           PREDICTED: RNA-dependent RNA polymerase 6 isoform X1
           [Solanum lycopersicum]
          Length = 1197

 Score = 61.2 bits (147), Expect = 3e-07
 Identities = 35/99 (35%), Positives = 49/99 (49%), Gaps = 10/99 (10%)
 Frame = -2

Query: 268 SIFPFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLFT------- 110
           +  P+ F D   +IG L     F+  W+    P S +NF + PFN  CK+LFT       
Sbjct: 125 TFMPYKFSDVGVEIGVLVSNDDFVVGWR---GPHSGVNFLVDPFNGKCKILFTRDTAFCF 181

Query: 109 ---GKFEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
              GK  VI+C  K++FLLR + E+    D    VL+ Q
Sbjct: 182 KGEGKHAVIKCNFKIEFLLREINEINECKDFSSLVLLLQ 220


>ref|NP_001266205.1| RNA-dependent RNA polymerase 6 [Solanum lycopersicum]
           gi|397529811|gb|AFO53516.1| RNA-dependent RNA polymerase
           6 [Solanum lycopersicum]
          Length = 1197

 Score = 61.2 bits (147), Expect = 3e-07
 Identities = 35/99 (35%), Positives = 49/99 (49%), Gaps = 10/99 (10%)
 Frame = -2

Query: 268 SIFPFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLFT------- 110
           +  P+ F D   +IG L     F+  W+    P S +NF + PFN  CK+LFT       
Sbjct: 125 TFMPYKFSDVGVEIGVLVSNDDFVVGWR---GPHSGVNFLVDPFNGKCKILFTRDTAFCF 181

Query: 109 ---GKFEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
              GK  VI+C  K++FLLR + E+    D    VL+ Q
Sbjct: 182 KGEGKHAVIKCNFKIEFLLREINEINECKDFSSLVLLLQ 220


>ref|XP_008778879.1| PREDICTED: LOW QUALITY PROTEIN: probable RNA-dependent RNA
           polymerase SHL2 [Phoenix dactylifera]
          Length = 1198

 Score = 60.5 bits (145), Expect = 5e-07
 Identities = 32/96 (33%), Positives = 51/96 (53%), Gaps = 10/96 (10%)
 Frame = -2

Query: 259 PFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLFTGK-------- 104
           PF F D   +IG+L     FL  WK    PKS ++F I PF+  C++LF+ +        
Sbjct: 132 PFRFTDVGVEIGTLASRDEFLVAWK---GPKSGVDFLIDPFDGRCRILFSKETAFAFKDI 188

Query: 103 --FEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
               V++C  K++FL R + E+K+  D+    ++FQ
Sbjct: 189 KEMAVLKCDFKVEFLARDINEVKLFTDQYPPAMLFQ 224


>ref|XP_009609663.1| PREDICTED: RNA-dependent RNA polymerase 6, partial [Nicotiana
           tomentosiformis]
          Length = 259

 Score = 60.1 bits (144), Expect = 6e-07
 Identities = 31/107 (28%), Positives = 55/107 (51%), Gaps = 10/107 (9%)
 Frame = -2

Query: 292 WRCRVKSSSIFPFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLF 113
           +R   +  +  PF F D   ++G L     F+  W+    P++ ++F + PFN +CK+LF
Sbjct: 117 YRLNQRRRTTMPFKFSDVSVEMGVLVSKDDFVVGWR---GPRTGVDFLVDPFNGTCKILF 173

Query: 112 T----------GKFEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
           T           +  +I+C  K++FL+R + E+K   D    V++FQ
Sbjct: 174 TKDTAFSFRGEARHAIIKCNFKIEFLVREINEIKKCKDFTSLVILFQ 220


>emb|CAR47810.1| RNA-dependent RNA polymerase 6 [Nicotiana tabacum]
          Length = 1197

 Score = 60.1 bits (144), Expect = 6e-07
 Identities = 31/107 (28%), Positives = 55/107 (51%), Gaps = 10/107 (9%)
 Frame = -2

Query: 292 WRCRVKSSSIFPFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLF 113
           +R   +  +  PF F D   ++G L     F+  W+    P++ ++F + PFN +CK+LF
Sbjct: 117 YRLNQRRRTTMPFKFSDVSVEMGVLVSKDDFVVGWR---GPRTGVDFLVDPFNGTCKILF 173

Query: 112 T----------GKFEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
           T           +  +I+C  K++FL+R + E+K   D    V++FQ
Sbjct: 174 TKDTAFSFRGEARHAIIKCNFKIEFLVREINEIKKCKDFTSLVILFQ 220


>gb|ADI52625.1| RNA-dependent RNA polymerase 6 [Nicotiana tabacum]
          Length = 1197

 Score = 60.1 bits (144), Expect = 6e-07
 Identities = 31/107 (28%), Positives = 55/107 (51%), Gaps = 10/107 (9%)
 Frame = -2

Query: 292 WRCRVKSSSIFPFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLF 113
           +R   +  +  PF F D   ++G L     F+  W+    P++ ++F + PFN +CK+LF
Sbjct: 117 YRLNQRRRTTMPFKFSDVSVEMGVLVSKDDFVVGWR---GPRTGVDFLVDPFNGTCKILF 173

Query: 112 T----------GKFEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
           T           +  +I+C  K++FL+R + E+K   D    V++FQ
Sbjct: 174 TKDTAFSFRGEARHAIIKCNFKIEFLVREINEIKKCKDFTSLVILFQ 220


>ref|XP_002282647.1| PREDICTED: RNA-dependent RNA polymerase 6 [Vitis vinifera]
           gi|731385864|ref|XP_010648660.1| PREDICTED:
           RNA-dependent RNA polymerase 6 [Vitis vinifera]
           gi|731385866|ref|XP_010648661.1| PREDICTED:
           RNA-dependent RNA polymerase 6 [Vitis vinifera]
          Length = 1197

 Score = 60.1 bits (144), Expect = 6e-07
 Identities = 32/97 (32%), Positives = 49/97 (50%), Gaps = 10/97 (10%)
 Frame = -2

Query: 262 FPFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLFT--------- 110
           FPF FPD   +IG L     +   W+    P S ++F + PF+ +CK LFT         
Sbjct: 128 FPFKFPDVRLEIGILVSRDEYFAGWR---GPSSGVDFLVDPFDGTCKFLFTKDTAFSFKG 184

Query: 109 -GKFEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
             K  VI+C  K++FL+R + E++   D    +L+ Q
Sbjct: 185 MPKHAVIKCNFKVEFLVREINEVRQRRDMSSLILLLQ 221


>gb|ACO72600.1| RNA-dependent RNA polymerase 6 [Nicotiana glutinosa]
          Length = 1197

 Score = 59.7 bits (143), Expect = 8e-07
 Identities = 31/107 (28%), Positives = 55/107 (51%), Gaps = 10/107 (9%)
 Frame = -2

Query: 292 WRCRVKSSSIFPFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLF 113
           +R   +  +  PF F D   ++G L     F+  W+    P++ ++F + PFN +CK+LF
Sbjct: 117 YRLNQRRRTTMPFKFSDVSVEMGVLVSKHDFVVGWR---GPRTGVDFLVDPFNGTCKILF 173

Query: 112 T----------GKFEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
           T           +  +I+C  K++FL+R + E+K   D M  V++ Q
Sbjct: 174 TKDTVFSFRGEARHAIIKCNFKIEFLVREINEIKKCKDFMSLVILLQ 220


>ref|XP_009389500.1| PREDICTED: LOW QUALITY PROTEIN: probable RNA-dependent RNA
           polymerase SHL2 [Musa acuminata subsp. malaccensis]
          Length = 1199

 Score = 59.3 bits (142), Expect = 1e-06
 Identities = 33/96 (34%), Positives = 52/96 (54%), Gaps = 10/96 (10%)
 Frame = -2

Query: 259 PFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLFTGK-------- 104
           PF F +A  +IG+L     FL +WK    P S ++F I PF++ CK+LF+ +        
Sbjct: 132 PFRFSNACVEIGNLVSRDEFLVSWK---GPDSAVDFVIDPFDDCCKILFSKETAFYFKGL 188

Query: 103 --FEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
               +I C  K++FLLR + E+++  D    V+ FQ
Sbjct: 189 REMTLIMCDFKVEFLLRDINEVRVHKDAAPFVMQFQ 224


>sp|Q8LHH9.1|SHL2_ORYSJ RecName: Full=Probable RNA-dependent RNA polymerase SHL2; AltName:
           Full=Protein SHOOTLESS 2 gi|21328146|dbj|BAC00725.1|
           putative RNA-dependent RNA polymerase [Oryza sativa
           Japonica Group] gi|157279691|dbj|BAF80151.1| SHOOTLESS2
           [Oryza sativa Japonica Group]
          Length = 1218

 Score = 59.3 bits (142), Expect = 1e-06
 Identities = 33/90 (36%), Positives = 52/90 (57%), Gaps = 10/90 (11%)
 Frame = -2

Query: 277 KSSSIFPFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLFT---- 110
           + SSI PF FPD   ++G+L  P  FL  W+    P + ++  + PF+  C+L+FT    
Sbjct: 143 RRSSIAPFRFPDVRLEVGALPSPGAFLAAWR---GPDAGLDLSVDPFDGCCRLVFTRDTA 199

Query: 109 ----GKFEV--IRCTLKLDFLLRGVMEMKI 38
               G  EV  IRC +KL+F +R V+E+++
Sbjct: 200 FTFPGFREVAAIRCDVKLEFPVRDVLEVRL 229


>gb|ACA13601.1| RNA-dependent RNA polymerase [Nicotiana attenuata]
          Length = 1197

 Score = 58.2 bits (139), Expect = 2e-06
 Identities = 30/107 (28%), Positives = 54/107 (50%), Gaps = 10/107 (9%)
 Frame = -2

Query: 292 WRCRVKSSSIFPFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLF 113
           +R   +  +  PF F D   ++G L     F+  W+    P++ ++F + PFN +CK+LF
Sbjct: 117 YRLNQRRRTTMPFKFSDVSIEMGVLVSKDDFVVGWR---GPRTGVDFLVDPFNGACKILF 173

Query: 112 T----------GKFEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
           T           +  +I+C  K++FL+R + E+K   D    V++ Q
Sbjct: 174 TKDTAFSFRGEARHAIIKCNFKIEFLVREINEIKKCKDFTSLVILLQ 220


>gb|AAU21242.1| putative RNA-dependent RNA polymerase SDE1 [Nicotiana benthamiana]
          Length = 1197

 Score = 57.8 bits (138), Expect = 3e-06
 Identities = 30/107 (28%), Positives = 54/107 (50%), Gaps = 10/107 (9%)
 Frame = -2

Query: 292 WRCRVKSSSIFPFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLF 113
           +R   +  +  PF F D   ++G L     F+  W+    P++ ++F + PFN +CK+LF
Sbjct: 117 YRLNQRRRTTMPFKFSDVSVEMGVLVSKDDFVVGWR---GPRTGVDFFVDPFNGACKILF 173

Query: 112 T----------GKFEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
           T           K  +I+C  K++F++R + E+K   D    V++ Q
Sbjct: 174 TKDTAFSFRGEAKHAIIKCNFKIEFMVREINEIKKFKDFTSLVILLQ 220


>ref|XP_008219491.1| PREDICTED: RNA-dependent RNA polymerase 6 [Prunus mume]
          Length = 1196

 Score = 57.0 bits (136), Expect = 5e-06
 Identities = 31/96 (32%), Positives = 48/96 (50%), Gaps = 10/96 (10%)
 Frame = -2

Query: 259 PFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLF----------T 110
           PF   D H +IGSL R   F  +W+    P   ++F + PF+ +CK  F          T
Sbjct: 128 PFKLSDVHVEIGSLVRQDEFFVSWR---GPPYGVDFIVDPFDGTCKFCFTMDTAFSFKGT 184

Query: 109 GKFEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
            K  VI+C  K++FL+R + E+K   D    V++ +
Sbjct: 185 KKHAVIKCDFKVEFLVREIAEIKQYTDTSYLVILLR 220


>ref|XP_002515520.1| RNA-dependent RNA polymerase, putative [Ricinus communis]
           gi|223545464|gb|EEF46969.1| RNA-dependent RNA
           polymerase, putative [Ricinus communis]
          Length = 1203

 Score = 57.0 bits (136), Expect = 5e-06
 Identities = 33/96 (34%), Positives = 47/96 (48%), Gaps = 10/96 (10%)
 Frame = -2

Query: 259 PFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLFTG--------- 107
           PF   D H +IG+L     FL  W+    P S ++F + PF+  CK  FT          
Sbjct: 129 PFKLSDVHVEIGTLVSRDEFLVGWR---GPPSGVDFLVDPFDGKCKFCFTRDTAFSFKGT 185

Query: 106 -KFEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
            +  VIRC  KL+FL+R + E+K   D    V++ Q
Sbjct: 186 TEHAVIRCDFKLEFLVRDINEIKQYTDTSCLVILLQ 221


>ref|XP_007225440.1| hypothetical protein PRUPE_ppa000422mg [Prunus persica]
           gi|462422376|gb|EMJ26639.1| hypothetical protein
           PRUPE_ppa000422mg [Prunus persica]
          Length = 1196

 Score = 56.6 bits (135), Expect = 7e-06
 Identities = 31/96 (32%), Positives = 48/96 (50%), Gaps = 10/96 (10%)
 Frame = -2

Query: 259 PFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLF----------T 110
           PF   D H +IGSL R   F  +W+    P   ++F + PF+ +CK  F          T
Sbjct: 128 PFKLSDVHVEIGSLVRQDEFFASWR---GPPYGVDFIVDPFDGTCKFCFTMDTAFSFKGT 184

Query: 109 GKFEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
            K  VI+C  K++FL+R + E+K   D    V++ +
Sbjct: 185 KKHAVIKCDFKVEFLVREIAEIKQYTDTSYLVILLR 220


>dbj|BAF96019.1| RNA-dependent RNA polymerase 6 [Nicotiana tabacum]
          Length = 1197

 Score = 56.6 bits (135), Expect = 7e-06
 Identities = 29/107 (27%), Positives = 54/107 (50%), Gaps = 10/107 (9%)
 Frame = -2

Query: 292 WRCRVKSSSIFPFSFPDAHFKIGSLTRPTHFLFTWKLDLKPKSNINFPIVPFNNSCKLLF 113
           +R   +  +  PF F D   ++G L     F+  W+    P++ ++F + PFN +CK+LF
Sbjct: 117 YRLNQRRRTTMPFKFSDVSVEMGVLVSKDDFVVGWR---GPRTGVDFFVDPFNGTCKILF 173

Query: 112 T----------GKFEVIRCTLKLDFLLRGVMEMKIDADRMQHVLMFQ 2
           T           +  +I+C  K++F++R + E+K   D    V++ Q
Sbjct: 174 TKDTAFSFRGEARHAIIKCNFKIEFMVREINEIKKCKDFTSLVILLQ 220