BLASTX nr result
ID: Mentha25_contig00039127
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Mentha25_contig00039127
(303 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EYU26033.1| hypothetical protein MIMGU_mgv1a003132mg [Mimulus... 134 1e-29
gb|EPS69322.1| hypothetical protein M569_05444, partial [Genlise... 120 2e-25
ref|XP_004167322.1| PREDICTED: LOW QUALITY PROTEIN: large subuni... 119 4e-25
ref|XP_004139607.1| PREDICTED: large subunit GTPase 1 homolog [C... 119 4e-25
ref|XP_004156028.1| PREDICTED: large subunit GTPase 1 homolog [C... 116 4e-24
ref|XP_004146392.1| PREDICTED: large subunit GTPase 1 homolog [C... 116 4e-24
ref|XP_004251223.1| PREDICTED: large subunit GTPase 1 homolog [S... 115 6e-24
ref|XP_006340326.1| PREDICTED: large subunit GTPase 1 homolog [S... 114 2e-23
ref|XP_002513727.1| GTP binding protein, putative [Ricinus commu... 110 2e-22
ref|XP_007015655.1| P-loop containing nucleoside triphosphate hy... 110 2e-22
ref|XP_006417692.1| hypothetical protein EUTSA_v10007194mg [Eutr... 109 5e-22
ref|XP_006303811.1| hypothetical protein CARUB_v10012258mg [Caps... 108 1e-21
ref|XP_006408622.1| hypothetical protein EUTSA_v10001955mg [Eutr... 106 3e-21
ref|NP_172317.1| P-loop containing nucleoside triphosphate hydro... 106 4e-21
dbj|BAF00519.1| hypothetical protein [Arabidopsis thaliana] 106 4e-21
ref|XP_002889691.1| GTP-binding family protein [Arabidopsis lyra... 105 7e-21
ref|XP_006378981.1| hypothetical protein POPTR_0009s02210g [Popu... 104 1e-20
ref|XP_002264570.1| PREDICTED: large subunit GTPase 1 homolog [V... 104 1e-20
ref|XP_004291536.1| PREDICTED: large subunit GTPase 1 homolog [F... 102 6e-20
ref|NP_180288.1| P-loop containing nucleoside triphosphate hydro... 102 6e-20
>gb|EYU26033.1| hypothetical protein MIMGU_mgv1a003132mg [Mimulus guttatus]
Length = 605
Score = 134 bits (338), Expect = 1e-29
Identities = 72/102 (70%), Positives = 81/102 (79%), Gaps = 2/102 (1%)
Frame = +3
Query: 3 YYRNQ-QKILESVTEVSDIEAVIEQADEAHRLFSTRSSPG-LPINLDSASSPNDMTLXXX 176
YYR+Q +K+LESVTE+SDIEAVIEQADEAHRL+S + PG L INLD AS N+MTL
Sbjct: 30 YYRSQHKKVLESVTEISDIEAVIEQADEAHRLYSAVNPPGKLTINLDPASRNNEMTLAER 89
Query: 177 XXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFLE 302
ALHASSLRIPRRPPWNA M+VEELDDNE+RAFLE
Sbjct: 90 SKEQKKEEALHASSLRIPRRPPWNANMSVEELDDNERRAFLE 131
>gb|EPS69322.1| hypothetical protein M569_05444, partial [Genlisea aurea]
Length = 217
Score = 120 bits (302), Expect = 2e-25
Identities = 67/102 (65%), Positives = 78/102 (76%), Gaps = 2/102 (1%)
Frame = +3
Query: 3 YYRNQQK-ILESVTEVSDIEAVIEQADEAHRLFST-RSSPGLPINLDSASSPNDMTLXXX 176
YYR+QQK ILESVT+V+DIEAVIE ADEA+R S SS +PI LDSASS N+ T
Sbjct: 30 YYRSQQKKILESVTDVNDIEAVIEAADEANRFLSNAESSLKIPIRLDSASSSNESTGEGR 89
Query: 177 XXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFLE 302
ALHASSLRIPRRPPWNAKM+VEEL++NE+RAFL+
Sbjct: 90 REQREREEALHASSLRIPRRPPWNAKMSVEELEENERRAFLD 131
>ref|XP_004167322.1| PREDICTED: LOW QUALITY PROTEIN: large subunit GTPase 1 homolog,
partial [Cucumis sativus]
Length = 573
Score = 119 bits (298), Expect = 4e-25
Identities = 62/101 (61%), Positives = 79/101 (78%), Gaps = 2/101 (1%)
Frame = +3
Query: 3 YYRNQQK-ILESVTEVSDIEAVIEQADEAHRLFSTRS-SPGLPINLDSASSPNDMTLXXX 176
+Y++QQK +LESVTEVSDI+AVI+QADEA RLFS + +P INLD +SS ++MTL
Sbjct: 15 FYKSQQKKVLESVTEVSDIDAVIQQADEAERLFSIDNPTPNFLINLDGSSSVSEMTLVER 74
Query: 177 XXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
ALHASSLR+PRRPPWNA+M+ EELDDNE+++FL
Sbjct: 75 RDQQKIEEALHASSLRVPRRPPWNARMSAEELDDNERQSFL 115
>ref|XP_004139607.1| PREDICTED: large subunit GTPase 1 homolog [Cucumis sativus]
Length = 588
Score = 119 bits (298), Expect = 4e-25
Identities = 62/101 (61%), Positives = 79/101 (78%), Gaps = 2/101 (1%)
Frame = +3
Query: 3 YYRNQQK-ILESVTEVSDIEAVIEQADEAHRLFSTRS-SPGLPINLDSASSPNDMTLXXX 176
+Y++QQK +LESVTEVSDI+AVI+QADEA RLFS + +P INLD +SS ++MTL
Sbjct: 30 FYKSQQKKVLESVTEVSDIDAVIQQADEAERLFSIDNPTPNFLINLDGSSSVSEMTLVER 89
Query: 177 XXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
ALHASSLR+PRRPPWNA+M+ EELDDNE+++FL
Sbjct: 90 RDQQKIEEALHASSLRVPRRPPWNARMSAEELDDNERQSFL 130
>ref|XP_004156028.1| PREDICTED: large subunit GTPase 1 homolog [Cucumis sativus]
Length = 361
Score = 116 bits (290), Expect = 4e-24
Identities = 61/101 (60%), Positives = 78/101 (77%), Gaps = 2/101 (1%)
Frame = +3
Query: 3 YYRNQQK-ILESVTEVSDIEAVIEQADEAHRLFSTRS-SPGLPINLDSASSPNDMTLXXX 176
+Y++QQK +LESVTEVSDI+AVI+QADEA RLFS + +P INLD +SS ++MT
Sbjct: 30 FYKSQQKKVLESVTEVSDIDAVIQQADEAERLFSIDNPTPNFLINLDGSSSISEMTPAER 89
Query: 177 XXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
ALHASSLR+PRRPPWNA+M+ EELDDNE+++FL
Sbjct: 90 REQQKIEEALHASSLRVPRRPPWNARMSAEELDDNERQSFL 130
>ref|XP_004146392.1| PREDICTED: large subunit GTPase 1 homolog [Cucumis sativus]
Length = 588
Score = 116 bits (290), Expect = 4e-24
Identities = 61/101 (60%), Positives = 78/101 (77%), Gaps = 2/101 (1%)
Frame = +3
Query: 3 YYRNQQK-ILESVTEVSDIEAVIEQADEAHRLFSTRS-SPGLPINLDSASSPNDMTLXXX 176
+Y++QQK +LESVTEVSDI+AVI+QADEA RLFS + +P INLD +SS ++MT
Sbjct: 30 FYKSQQKKVLESVTEVSDIDAVIQQADEAERLFSIDNPTPNFLINLDGSSSISEMTPAER 89
Query: 177 XXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
ALHASSLR+PRRPPWNA+M+ EELDDNE+++FL
Sbjct: 90 REQQKIEEALHASSLRVPRRPPWNARMSAEELDDNERQSFL 130
>ref|XP_004251223.1| PREDICTED: large subunit GTPase 1 homolog [Solanum lycopersicum]
Length = 589
Score = 115 bits (288), Expect = 6e-24
Identities = 62/100 (62%), Positives = 73/100 (73%), Gaps = 2/100 (2%)
Frame = +3
Query: 6 YRNQ-QKILESVTEVSDIEAVIEQADEAHRLFSTRSSP-GLPINLDSASSPNDMTLXXXX 179
YRN +K+LESVTEV+D+EAVIEQ DEAHRL+S + P L +NLDS SS DM
Sbjct: 31 YRNHNKKVLESVTEVTDLEAVIEQTDEAHRLYSDLNPPVNLLVNLDSGSSSGDMRPEERL 90
Query: 180 XXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
ALHASSLR+PRRPPWN KM+VEELD NE++AFL
Sbjct: 91 KQQKKEEALHASSLRVPRRPPWNTKMSVEELDINERQAFL 130
>ref|XP_006340326.1| PREDICTED: large subunit GTPase 1 homolog [Solanum tuberosum]
Length = 585
Score = 114 bits (284), Expect = 2e-23
Identities = 61/100 (61%), Positives = 73/100 (73%), Gaps = 2/100 (2%)
Frame = +3
Query: 6 YRNQ-QKILESVTEVSDIEAVIEQADEAHRLFSTRSSP-GLPINLDSASSPNDMTLXXXX 179
YRN +K+LESVTEV+D+EAVIEQ DEAHRL+S + P L +NLDS SS DM
Sbjct: 31 YRNHNKKVLESVTEVTDLEAVIEQTDEAHRLYSDLNPPVNLLVNLDSGSSTGDMGPEERL 90
Query: 180 XXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
ALHASSLR+PRRPPWN +M+VEELD NE++AFL
Sbjct: 91 KQQKKEEALHASSLRVPRRPPWNTRMSVEELDINERQAFL 130
>ref|XP_002513727.1| GTP binding protein, putative [Ricinus communis]
gi|223547178|gb|EEF48674.1| GTP binding protein,
putative [Ricinus communis]
Length = 596
Score = 110 bits (276), Expect = 2e-22
Identities = 62/104 (59%), Positives = 77/104 (74%), Gaps = 5/104 (4%)
Frame = +3
Query: 3 YYRN-QQKILESVTEVSDIEAVIEQADEAHRLFSTR---SSPGLPINLD-SASSPNDMTL 167
YY + +K+LESVTEVSDI+AVIEQA+EA LFS + ++P PI+LD S+S +DMT
Sbjct: 30 YYNSLHKKVLESVTEVSDIDAVIEQAEEADLLFSHQLPAAAPNQPISLDVSSSGISDMTP 89
Query: 168 XXXXXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
ALHASSLR+PRRPPWNA+M++EELDDNEK AFL
Sbjct: 90 KERREQQKKEEALHASSLRVPRRPPWNAEMSIEELDDNEKHAFL 133
>ref|XP_007015655.1| P-loop containing nucleoside triphosphate hydrolases superfamily
protein [Theobroma cacao] gi|508786018|gb|EOY33274.1|
P-loop containing nucleoside triphosphate hydrolases
superfamily protein [Theobroma cacao]
Length = 591
Score = 110 bits (275), Expect = 2e-22
Identities = 61/101 (60%), Positives = 77/101 (76%), Gaps = 2/101 (1%)
Frame = +3
Query: 3 YYRNQ-QKILESVTEVSDIEAVIEQADEAHRLFSTRS-SPGLPINLDSASSPNDMTLXXX 176
+Y++Q +K+LESVTEVSDI+AVIEQA+EA +LFS + +P L INLD++SS + MT
Sbjct: 30 FYKSQHKKVLESVTEVSDIDAVIEQAEEADQLFSIQHPTPNLLINLDTSSSTSGMTPEER 89
Query: 177 XXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
ALHASSLR+PRRP WNA M+VEELD NEK+AFL
Sbjct: 90 REQQKKEEALHASSLRVPRRPSWNAGMSVEELDANEKQAFL 130
>ref|XP_006417692.1| hypothetical protein EUTSA_v10007194mg [Eutrema salsugineum]
gi|557095463|gb|ESQ36045.1| hypothetical protein
EUTSA_v10007194mg [Eutrema salsugineum]
Length = 577
Score = 109 bits (272), Expect = 5e-22
Identities = 59/102 (57%), Positives = 74/102 (72%), Gaps = 3/102 (2%)
Frame = +3
Query: 3 YYRN-QQKILESVTEVSDIEAVIEQADEAHRLFSTRSS--PGLPINLDSASSPNDMTLXX 173
YY+N Q+K+LESVTEVSDI+A+IEQA+EA RLF+ S LPINLD+ SS + +
Sbjct: 30 YYKNLQKKVLESVTEVSDIDAIIEQAEEAERLFTINHSNTTPLPINLDTNSSSSTVAAEE 89
Query: 174 XXXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
ALHASSL++PRRPPW +M+VEELD NEK+AFL
Sbjct: 90 WREQQKIEEALHASSLQVPRRPPWTPEMSVEELDANEKQAFL 131
>ref|XP_006303811.1| hypothetical protein CARUB_v10012258mg [Capsella rubella]
gi|482572522|gb|EOA36709.1| hypothetical protein
CARUB_v10012258mg [Capsella rubella]
Length = 589
Score = 108 bits (269), Expect = 1e-21
Identities = 58/102 (56%), Positives = 74/102 (72%), Gaps = 3/102 (2%)
Frame = +3
Query: 3 YYRN-QQKILESVTEVSDIEAVIEQADEAHRLFSTRSSPGLP--INLDSASSPNDMTLXX 173
YY+N Q+K+LESVTEVSDI+A+IEQA+EA RL++ S P INLD+ SS + +T
Sbjct: 30 YYKNLQKKVLESVTEVSDIDAIIEQAEEAERLYTINHSSATPLSINLDTNSSSSVITAEE 89
Query: 174 XXXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
ALHASSL++PRRPPW +M+VEELD NEK+AFL
Sbjct: 90 WREQQKIEEALHASSLQVPRRPPWTPEMSVEELDANEKQAFL 131
>ref|XP_006408622.1| hypothetical protein EUTSA_v10001955mg [Eutrema salsugineum]
gi|557109778|gb|ESQ50075.1| hypothetical protein
EUTSA_v10001955mg [Eutrema salsugineum]
Length = 532
Score = 106 bits (265), Expect = 3e-21
Identities = 56/102 (54%), Positives = 75/102 (73%), Gaps = 3/102 (2%)
Frame = +3
Query: 3 YYRNQ-QKILESVTEVSDIEAVIEQADEAHRLFSTR--SSPGLPINLDSASSPNDMTLXX 173
YY+ Q +K+LESVTEV+DI+A+IEQA+EA RLF+ S+ +PINL++ASS + +T
Sbjct: 30 YYKTQNKKVLESVTEVTDIDAIIEQAEEAERLFAIHHDSATPVPINLETASSSSGVTAKE 89
Query: 174 XXXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
ALHASSL++PRRPPW KM V++LD NEK+AFL
Sbjct: 90 WKEQRKREEALHASSLQVPRRPPWTPKMNVDQLDINEKQAFL 131
>ref|NP_172317.1| P-loop containing nucleoside triphosphate hydrolases superfamily
protein [Arabidopsis thaliana]
gi|6664306|gb|AAF22888.1|AC006932_5 T27G7.9 [Arabidopsis
thaliana] gi|66792666|gb|AAY56435.1| At1g08410
[Arabidopsis thaliana] gi|133778880|gb|ABO38780.1|
At1g08410 [Arabidopsis thaliana]
gi|332190165|gb|AEE28286.1| P-loop containing nucleoside
triphosphate hydrolases superfamily protein [Arabidopsis
thaliana]
Length = 589
Score = 106 bits (264), Expect = 4e-21
Identities = 57/102 (55%), Positives = 73/102 (71%), Gaps = 3/102 (2%)
Frame = +3
Query: 3 YYRN-QQKILESVTEVSDIEAVIEQADEAHRLFSTRSSPGLP--INLDSASSPNDMTLXX 173
YY+N Q+K+LESVTEVSDI+A+IEQA+EA RL++ S P INLD+ SS + +
Sbjct: 30 YYKNLQKKVLESVTEVSDIDAIIEQAEEAERLYTINHSSSTPLSINLDTNSSSSVIAAEE 89
Query: 174 XXXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
ALHASSL++PRRPPW +M+VEELD NEK+AFL
Sbjct: 90 WREQQKIEEALHASSLQVPRRPPWTPEMSVEELDANEKQAFL 131
>dbj|BAF00519.1| hypothetical protein [Arabidopsis thaliana]
Length = 377
Score = 106 bits (264), Expect = 4e-21
Identities = 57/102 (55%), Positives = 73/102 (71%), Gaps = 3/102 (2%)
Frame = +3
Query: 3 YYRN-QQKILESVTEVSDIEAVIEQADEAHRLFSTRSSPGLP--INLDSASSPNDMTLXX 173
YY+N Q+K+LESVTEVSDI+A+IEQA+EA RL++ S P INLD+ SS + +
Sbjct: 30 YYKNLQKKVLESVTEVSDIDAIIEQAEEAERLYTINHSSSTPLSINLDTNSSSSVIAAEE 89
Query: 174 XXXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
ALHASSL++PRRPPW +M+VEELD NEK+AFL
Sbjct: 90 WREQQKIEEALHASSLQVPRRPPWTPEMSVEELDANEKQAFL 131
>ref|XP_002889691.1| GTP-binding family protein [Arabidopsis lyrata subsp. lyrata]
gi|297335533|gb|EFH65950.1| GTP-binding family protein
[Arabidopsis lyrata subsp. lyrata]
Length = 583
Score = 105 bits (262), Expect = 7e-21
Identities = 57/102 (55%), Positives = 73/102 (71%), Gaps = 3/102 (2%)
Frame = +3
Query: 3 YYRN-QQKILESVTEVSDIEAVIEQADEAHRLFSTRSSPGLP--INLDSASSPNDMTLXX 173
YY+N Q+K+LESVTEVSDI+A+IEQA+EA RL++ S P INLD+ SS + +
Sbjct: 30 YYKNLQKKVLESVTEVSDIDAIIEQAEEAERLYTINHSSSTPLSINLDTNSSSSVIAPEE 89
Query: 174 XXXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
ALHASSL++PRRPPW +M+VEELD NEK+AFL
Sbjct: 90 WREQQKIEEALHASSLQVPRRPPWTPEMSVEELDANEKQAFL 131
>ref|XP_006378981.1| hypothetical protein POPTR_0009s02210g [Populus trichocarpa]
gi|550330867|gb|ERP56778.1| hypothetical protein
POPTR_0009s02210g [Populus trichocarpa]
Length = 602
Score = 104 bits (259), Expect = 1e-20
Identities = 59/106 (55%), Positives = 74/106 (69%), Gaps = 7/106 (6%)
Frame = +3
Query: 3 YYRNQ-QKILESVTEVSDIEAVIEQADEAHRLFSTRSS------PGLPINLDSASSPNDM 161
YY++Q +K+LES T+V++I+AV+EQ DE LFS+ SS P L INLD+ S +DM
Sbjct: 30 YYKSQHKKVLESFTDVTEIDAVMEQVDEGE-LFSSSSSSVHHPVPNLLINLDTGSGVSDM 88
Query: 162 TLXXXXXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
T ALHASSLR+PRRPPWNA M+VEELD NEK+AFL
Sbjct: 89 TAEEKREKQKKEEALHASSLRVPRRPPWNAGMSVEELDANEKQAFL 134
>ref|XP_002264570.1| PREDICTED: large subunit GTPase 1 homolog [Vitis vinifera]
Length = 597
Score = 104 bits (259), Expect = 1e-20
Identities = 54/101 (53%), Positives = 74/101 (73%), Gaps = 2/101 (1%)
Frame = +3
Query: 3 YYRNQ-QKILESVTEVSDIEAVIEQADEAHRLFS-TRSSPGLPINLDSASSPNDMTLXXX 176
+Y+NQ +K+LES+T+V+DI+AVI++ADEA RLF+ +P + INLD+ +S ++MT
Sbjct: 30 FYKNQHKKVLESITDVNDIDAVIQEADEAQRLFAFDHPAPNVLINLDTNASTSNMTDEER 89
Query: 177 XXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
ALHASSLR+PRRPPWN MT EELD NE++A L
Sbjct: 90 REQQKKEEALHASSLRVPRRPPWNVGMTAEELDANERQALL 130
>ref|XP_004291536.1| PREDICTED: large subunit GTPase 1 homolog [Fragaria vesca subsp.
vesca]
Length = 585
Score = 102 bits (254), Expect = 6e-20
Identities = 58/101 (57%), Positives = 72/101 (71%), Gaps = 2/101 (1%)
Frame = +3
Query: 3 YYRNQQKILESVTEVSDIEAVIEQADEAHRLFSTRS-SPGLPINLD-SASSPNDMTLXXX 176
Y + Q+K+LESVTEVSDI+AVIEQADEA RLFS + +P L INLD SA +P
Sbjct: 32 YRKMQKKVLESVTEVSDIDAVIEQADEADRLFSLNNPNPNLLINLDGSAIAPEQRR---- 87
Query: 177 XXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
A+HA SL++PRRPPW +M+VEELDDNE++AFL
Sbjct: 88 -EQQRKEEAVHAGSLQVPRRPPWTPEMSVEELDDNERQAFL 127
>ref|NP_180288.1| P-loop containing nucleoside triphosphate hydrolase-like protein
[Arabidopsis thaliana] gi|4646218|gb|AAD26884.1|
putative nucleotide-binding protein [Arabidopsis
thaliana] gi|63003826|gb|AAY25442.1| At2g27200
[Arabidopsis thaliana] gi|330252858|gb|AEC07952.1|
P-loop containing nucleoside triphosphate hydrolase-like
protein [Arabidopsis thaliana]
Length = 537
Score = 102 bits (254), Expect = 6e-20
Identities = 55/101 (54%), Positives = 73/101 (72%), Gaps = 3/101 (2%)
Frame = +3
Query: 6 YRNQ-QKILESVTEVSDIEAVIEQADEAHRLFSTR--SSPGLPINLDSASSPNDMTLXXX 176
Y++Q +K+LESVTEVSDI+A+IEQA+EA RLF+ S+ +PIN+D+ SS + +T
Sbjct: 31 YKDQHKKVLESVTEVSDIDAIIEQAEEAERLFAIHHDSATPVPINMDTGSSSSGITAKEW 90
Query: 177 XXXXXXXXALHASSLRIPRRPPWNAKMTVEELDDNEKRAFL 299
ALHASSL++PRRP W KM VE+LD NEK+AFL
Sbjct: 91 KEQRMREEALHASSLQVPRRPHWTPKMNVEKLDANEKQAFL 131