BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Mentha25_contig00019773
         (316 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI30940.3| unnamed protein product [Vitis vinifera]              160   1e-37
ref|NP_001268217.1| CBL-interacting protein kinase 04 [Vitis vin...   156   2e-36
emb|CAN72580.1| hypothetical protein VITISV_034332 [Vitis vinife...   156   2e-36
gb|EYU33397.1| hypothetical protein MIMGU_mgv1a005956mg [Mimulus...   150   2e-34
gb|AAG50566.1|AC073506_8 serine/threonine kinase, putative [Arab...   148   9e-34
ref|NP_564353.1| CBL-interacting serine/threonine-protein kinase...   148   9e-34
gb|AGA37204.1| CBL-interacting protein kinase 23.1 [Brassica napus]   147   1e-33
ref|XP_006306367.1| hypothetical protein CARUB_v10012271mg [Caps...   147   1e-33
ref|XP_006468060.1| PREDICTED: CBL-interacting serine/threonine-...   147   2e-33
ref|XP_006449000.1| hypothetical protein CICLE_v10015157mg [Citr...   147   2e-33
ref|XP_006448999.1| hypothetical protein CICLE_v10015157mg [Citr...   147   2e-33
ref|XP_006448996.1| hypothetical protein CICLE_v10015157mg [Citr...   147   2e-33
ref|XP_006361547.1| PREDICTED: CBL-interacting serine/threonine-...   144   1e-32
ref|XP_006361546.1| PREDICTED: CBL-interacting serine/threonine-...   144   1e-32
ref|XP_006389415.1| hypothetical protein POPTR_0025s00500g [Popu...   144   1e-32
ref|XP_007213911.1| hypothetical protein PRUPE_ppa005492mg [Prun...   144   1e-32
ref|XP_004231542.1| PREDICTED: CBL-interacting serine/threonine-...   144   1e-32
ref|XP_004231541.1| PREDICTED: CBL-interacting serine/threonine-...   144   1e-32
ref|XP_006389416.1| CBL-interacting protein kinase 23 [Populus t...   144   1e-32
ref|XP_006415491.1| hypothetical protein EUTSA_v10007518mg [Eutr...   144   1e-32

>emb|CBI30940.3| unnamed protein product [Vitis vinifera]
          Length = 455

 Score =  160 bits (406), Expect = 1e-37
 Identities = 76/89 (85%), Positives = 85/89 (95%)
 Frame = +1

Query: 4   PMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYKN 183
           P+GFDVKKNN+KMKL GEK+GRKGHLSIATEIFEVAPSL+MVELRK+GGDTLEFHKFYKN
Sbjct: 367 PLGFDVKKNNFKMKLQGEKTGRKGHLSIATEIFEVAPSLYMVELRKSGGDTLEFHKFYKN 426

Query: 184 LSTGLKDIVWKTGDEVKEEGNNGTDVSTT 270
           LSTGLKDIVWK+GDE KEE NNG+ V++T
Sbjct: 427 LSTGLKDIVWKSGDESKEEVNNGSGVAST 455


>ref|NP_001268217.1| CBL-interacting protein kinase 04 [Vitis vinifera]
           gi|310913178|emb|CBW30552.1| CBL-interacting protein
           kinase 04 [Vitis vinifera]
          Length = 449

 Score =  156 bits (395), Expect = 2e-36
 Identities = 74/83 (89%), Positives = 80/83 (96%)
 Frame = +1

Query: 4   PMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYKN 183
           P+GFDVKKNN+KMKL GEK+GRKGHLSIATEIFEVAPSL+MVELRK+GGDTLEFHKFYKN
Sbjct: 367 PLGFDVKKNNFKMKLQGEKTGRKGHLSIATEIFEVAPSLYMVELRKSGGDTLEFHKFYKN 426

Query: 184 LSTGLKDIVWKTGDEVKEEGNNG 252
           LSTGLKDIVWK+GDE KEE NNG
Sbjct: 427 LSTGLKDIVWKSGDESKEEVNNG 449


>emb|CAN72580.1| hypothetical protein VITISV_034332 [Vitis vinifera]
           gi|229609809|gb|ACQ83520.1| CBL-interacting protein
           kinase 04 [Vitis vinifera]
          Length = 462

 Score =  156 bits (395), Expect = 2e-36
 Identities = 74/83 (89%), Positives = 80/83 (96%)
 Frame = +1

Query: 4   PMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYKN 183
           P+GFDVKKNN+KMKL GEK+GRKGHLSIATEIFEVAPSL+MVELRK+GGDTLEFHKFYKN
Sbjct: 380 PLGFDVKKNNFKMKLQGEKTGRKGHLSIATEIFEVAPSLYMVELRKSGGDTLEFHKFYKN 439

Query: 184 LSTGLKDIVWKTGDEVKEEGNNG 252
           LSTGLKDIVWK+GDE KEE NNG
Sbjct: 440 LSTGLKDIVWKSGDESKEEVNNG 462


>gb|EYU33397.1| hypothetical protein MIMGU_mgv1a005956mg [Mimulus guttatus]
          Length = 463

 Score =  150 bits (379), Expect = 2e-34
 Identities = 70/79 (88%), Positives = 74/79 (93%)
 Frame = +1

Query: 4   PMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYKN 183
           PMGFDVKKNNYKMKL+GEKSGRKGHL+I TEIFEVAPSLHMVELRK+GGDTLEFHKFYKN
Sbjct: 382 PMGFDVKKNNYKMKLHGEKSGRKGHLAITTEIFEVAPSLHMVELRKSGGDTLEFHKFYKN 441

Query: 184 LSTGLKDIVWKTGDEVKEE 240
           LS GLKDIVWKTGDE   +
Sbjct: 442 LSIGLKDIVWKTGDEANND 460


>gb|AAG50566.1|AC073506_8 serine/threonine kinase, putative [Arabidopsis thaliana]
          Length = 480

 Score =  148 bits (373), Expect = 9e-34
 Identities = 69/84 (82%), Positives = 78/84 (92%)
 Frame = +1

Query: 1   APMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYK 180
           APMGFDVK NNYKMKL GEKSGRKG L++ATE+F+VAPSL+MVE+RK+GGDTLEFHKFYK
Sbjct: 380 APMGFDVKTNNYKMKLTGEKSGRKGQLAVATEVFQVAPSLYMVEMRKSGGDTLEFHKFYK 439

Query: 181 NLSTGLKDIVWKTGDEVKEEGNNG 252
           NL+TGLKDIVWKT DE KEEG +G
Sbjct: 440 NLTTGLKDIVWKTIDEEKEEGTDG 463


>ref|NP_564353.1| CBL-interacting serine/threonine-protein kinase 23 [Arabidopsis
           thaliana] gi|75331633|sp|Q93VD3.1|CIPKN_ARATH RecName:
           Full=CBL-interacting serine/threonine-protein kinase 23;
           AltName: Full=Protein LOW-K(+)-SENSITIVE 1; AltName:
           Full=SNF1-related kinase 3.23; AltName: Full=SOS2-like
           protein kinase PKS17 gi|14486386|gb|AAK61494.1|
           CBL-interacting protein kinase 23 [Arabidopsis thaliana]
           gi|15912283|gb|AAL08275.1| At1g30270/F12P21_6
           [Arabidopsis thaliana] gi|19699234|gb|AAL90983.1|
           At1g30270/F12P21_6 [Arabidopsis thaliana]
           gi|332193080|gb|AEE31201.1| CBL-interacting
           serine/threonine-protein kinase 23 [Arabidopsis
           thaliana]
          Length = 482

 Score =  148 bits (373), Expect = 9e-34
 Identities = 69/84 (82%), Positives = 78/84 (92%)
 Frame = +1

Query: 1   APMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYK 180
           APMGFDVK NNYKMKL GEKSGRKG L++ATE+F+VAPSL+MVE+RK+GGDTLEFHKFYK
Sbjct: 382 APMGFDVKTNNYKMKLTGEKSGRKGQLAVATEVFQVAPSLYMVEMRKSGGDTLEFHKFYK 441

Query: 181 NLSTGLKDIVWKTGDEVKEEGNNG 252
           NL+TGLKDIVWKT DE KEEG +G
Sbjct: 442 NLTTGLKDIVWKTIDEEKEEGTDG 465


>gb|AGA37204.1| CBL-interacting protein kinase 23.1 [Brassica napus]
          Length = 482

 Score =  147 bits (372), Expect = 1e-33
 Identities = 68/83 (81%), Positives = 77/83 (92%)
 Frame = +1

Query: 4   PMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYKN 183
           P+GFDVKKNNYKMKL GEKSGRKG L++ATE+F+VAPSL+MVE+RK+GGDTLEFHKFYKN
Sbjct: 386 PLGFDVKKNNYKMKLLGEKSGRKGQLAVATEVFQVAPSLYMVEMRKSGGDTLEFHKFYKN 445

Query: 184 LSTGLKDIVWKTGDEVKEEGNNG 252
           L+TGLKDIVWKT DE KEEG  G
Sbjct: 446 LTTGLKDIVWKTIDEEKEEGTEG 468


>ref|XP_006306367.1| hypothetical protein CARUB_v10012271mg [Capsella rubella]
           gi|482575078|gb|EOA39265.1| hypothetical protein
           CARUB_v10012271mg [Capsella rubella]
          Length = 484

 Score =  147 bits (371), Expect = 1e-33
 Identities = 72/88 (81%), Positives = 80/88 (90%)
 Frame = +1

Query: 1   APMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYK 180
           APMGFDVK NNYKMKL GEKSGRKG L++ATE+F+VAPSL+MVE+RK+GGDTLEFHKFYK
Sbjct: 383 APMGFDVKTNNYKMKLTGEKSGRKGQLAVATEVFQVAPSLYMVEMRKSGGDTLEFHKFYK 442

Query: 181 NLSTGLKDIVWKTGDEVKEEGNNGTDVS 264
           NL+TGLKDIVWKT DE KEE   GTDVS
Sbjct: 443 NLTTGLKDIVWKTIDEEKEE---GTDVS 467


>ref|XP_006468060.1| PREDICTED: CBL-interacting serine/threonine-protein kinase 23-like
           isoform X1 [Citrus sinensis]
          Length = 457

 Score =  147 bits (370), Expect = 2e-33
 Identities = 67/82 (81%), Positives = 78/82 (95%)
 Frame = +1

Query: 1   APMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYK 180
           +P+GFDVKKNN+K+KL GEK+GRKGHLS+ATEIFEVAPSL+MVELRK+GGDTLEFHKFYK
Sbjct: 367 SPLGFDVKKNNFKLKLQGEKTGRKGHLSVATEIFEVAPSLYMVELRKSGGDTLEFHKFYK 426

Query: 181 NLSTGLKDIVWKTGDEVKEEGN 246
           NLSTGLKD+VWK+GDE   EG+
Sbjct: 427 NLSTGLKDVVWKSGDETIVEGS 448


>ref|XP_006449000.1| hypothetical protein CICLE_v10015157mg [Citrus clementina]
           gi|557551611|gb|ESR62240.1| hypothetical protein
           CICLE_v10015157mg [Citrus clementina]
          Length = 459

 Score =  147 bits (370), Expect = 2e-33
 Identities = 67/82 (81%), Positives = 78/82 (95%)
 Frame = +1

Query: 1   APMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYK 180
           +P+GFDVKKNN+K+KL GEK+GRKGHLS+ATEIFEVAPSL+MVELRK+GGDTLEFHKFYK
Sbjct: 367 SPLGFDVKKNNFKLKLQGEKTGRKGHLSVATEIFEVAPSLYMVELRKSGGDTLEFHKFYK 426

Query: 181 NLSTGLKDIVWKTGDEVKEEGN 246
           NLSTGLKD+VWK+GDE   EG+
Sbjct: 427 NLSTGLKDVVWKSGDETIVEGS 448


>ref|XP_006448999.1| hypothetical protein CICLE_v10015157mg [Citrus clementina]
           gi|557551610|gb|ESR62239.1| hypothetical protein
           CICLE_v10015157mg [Citrus clementina]
          Length = 433

 Score =  147 bits (370), Expect = 2e-33
 Identities = 67/82 (81%), Positives = 78/82 (95%)
 Frame = +1

Query: 1   APMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYK 180
           +P+GFDVKKNN+K+KL GEK+GRKGHLS+ATEIFEVAPSL+MVELRK+GGDTLEFHKFYK
Sbjct: 343 SPLGFDVKKNNFKLKLQGEKTGRKGHLSVATEIFEVAPSLYMVELRKSGGDTLEFHKFYK 402

Query: 181 NLSTGLKDIVWKTGDEVKEEGN 246
           NLSTGLKD+VWK+GDE   EG+
Sbjct: 403 NLSTGLKDVVWKSGDETIVEGS 424


>ref|XP_006448996.1| hypothetical protein CICLE_v10015157mg [Citrus clementina]
           gi|567913367|ref|XP_006448997.1| hypothetical protein
           CICLE_v10015157mg [Citrus clementina]
           gi|567913369|ref|XP_006448998.1| hypothetical protein
           CICLE_v10015157mg [Citrus clementina]
           gi|567913377|ref|XP_006449002.1| hypothetical protein
           CICLE_v10015157mg [Citrus clementina]
           gi|557551607|gb|ESR62236.1| hypothetical protein
           CICLE_v10015157mg [Citrus clementina]
           gi|557551608|gb|ESR62237.1| hypothetical protein
           CICLE_v10015157mg [Citrus clementina]
           gi|557551609|gb|ESR62238.1| hypothetical protein
           CICLE_v10015157mg [Citrus clementina]
           gi|557551613|gb|ESR62242.1| hypothetical protein
           CICLE_v10015157mg [Citrus clementina]
          Length = 457

 Score =  147 bits (370), Expect = 2e-33
 Identities = 67/82 (81%), Positives = 78/82 (95%)
 Frame = +1

Query: 1   APMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYK 180
           +P+GFDVKKNN+K+KL GEK+GRKGHLS+ATEIFEVAPSL+MVELRK+GGDTLEFHKFYK
Sbjct: 367 SPLGFDVKKNNFKLKLQGEKTGRKGHLSVATEIFEVAPSLYMVELRKSGGDTLEFHKFYK 426

Query: 181 NLSTGLKDIVWKTGDEVKEEGN 246
           NLSTGLKD+VWK+GDE   EG+
Sbjct: 427 NLSTGLKDVVWKSGDETIVEGS 448


>ref|XP_006361547.1| PREDICTED: CBL-interacting serine/threonine-protein kinase 23-like
           isoform X2 [Solanum tuberosum]
          Length = 451

 Score =  144 bits (364), Expect = 1e-32
 Identities = 67/83 (80%), Positives = 77/83 (92%)
 Frame = +1

Query: 4   PMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYKN 183
           P+GF+V+KNNYK+KL GEKSGRKGHL++ATEI+EVAPSL+MVELRKAGGDTLEFHKFYKN
Sbjct: 369 PLGFNVRKNNYKIKLQGEKSGRKGHLNVATEIYEVAPSLYMVELRKAGGDTLEFHKFYKN 428

Query: 184 LSTGLKDIVWKTGDEVKEEGNNG 252
           LSTGLKDIVW+ GDE  EE  +G
Sbjct: 429 LSTGLKDIVWQLGDEAGEEVKDG 451


>ref|XP_006361546.1| PREDICTED: CBL-interacting serine/threonine-protein kinase 23-like
           isoform X1 [Solanum tuberosum]
          Length = 455

 Score =  144 bits (364), Expect = 1e-32
 Identities = 67/83 (80%), Positives = 77/83 (92%)
 Frame = +1

Query: 4   PMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYKN 183
           P+GF+V+KNNYK+KL GEKSGRKGHL++ATEI+EVAPSL+MVELRKAGGDTLEFHKFYKN
Sbjct: 369 PLGFNVRKNNYKIKLQGEKSGRKGHLNVATEIYEVAPSLYMVELRKAGGDTLEFHKFYKN 428

Query: 184 LSTGLKDIVWKTGDEVKEEGNNG 252
           LSTGLKDIVW+ GDE  EE  +G
Sbjct: 429 LSTGLKDIVWQLGDEAGEEVKDG 451


>ref|XP_006389415.1| hypothetical protein POPTR_0025s00500g [Populus trichocarpa]
           gi|550312209|gb|ERP48329.1| hypothetical protein
           POPTR_0025s00500g [Populus trichocarpa]
          Length = 473

 Score =  144 bits (364), Expect = 1e-32
 Identities = 69/86 (80%), Positives = 77/86 (89%)
 Frame = +1

Query: 1   APMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYK 180
           AP+GFDVKKNN+KMKL G+K GRKG LS+ATEIFEVAPSL+MVE+RK+GGDTLEFHKFYK
Sbjct: 380 APLGFDVKKNNFKMKLQGDKDGRKGRLSVATEIFEVAPSLYMVEVRKSGGDTLEFHKFYK 439

Query: 181 NLSTGLKDIVWKTGDEVKEEGNNGTD 258
           NLSTGLKDIVWKT DE KEE    T+
Sbjct: 440 NLSTGLKDIVWKTIDEEKEEEEAATN 465


>ref|XP_007213911.1| hypothetical protein PRUPE_ppa005492mg [Prunus persica]
           gi|462409776|gb|EMJ15110.1| hypothetical protein
           PRUPE_ppa005492mg [Prunus persica]
          Length = 458

 Score =  144 bits (364), Expect = 1e-32
 Identities = 66/83 (79%), Positives = 77/83 (92%)
 Frame = +1

Query: 4   PMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYKN 183
           P+GF VKKNN+K+KL GEK+GRKGHLS+ATEIFEVAPSL+MVELRK+GGDTLEFH FYKN
Sbjct: 368 PLGFGVKKNNFKLKLQGEKTGRKGHLSVATEIFEVAPSLYMVELRKSGGDTLEFHNFYKN 427

Query: 184 LSTGLKDIVWKTGDEVKEEGNNG 252
           LSTGLKDIVWK+GDE ++E  +G
Sbjct: 428 LSTGLKDIVWKSGDESRKEAEDG 450


>ref|XP_004231542.1| PREDICTED: CBL-interacting serine/threonine-protein kinase 23-like
           isoform 2 [Solanum lycopersicum]
          Length = 453

 Score =  144 bits (364), Expect = 1e-32
 Identities = 67/83 (80%), Positives = 77/83 (92%)
 Frame = +1

Query: 4   PMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYKN 183
           P+GF+V+KNNYK+KL GEKSGRKGHL++ATEI+EVAPSL+MVELRKAGGDTLEFHKFYKN
Sbjct: 371 PLGFNVRKNNYKIKLQGEKSGRKGHLNVATEIYEVAPSLYMVELRKAGGDTLEFHKFYKN 430

Query: 184 LSTGLKDIVWKTGDEVKEEGNNG 252
           LSTGLKDIVW+ GDE  EE  +G
Sbjct: 431 LSTGLKDIVWQLGDEAGEEVKDG 453


>ref|XP_004231541.1| PREDICTED: CBL-interacting serine/threonine-protein kinase 23-like
           isoform 1 [Solanum lycopersicum]
          Length = 457

 Score =  144 bits (364), Expect = 1e-32
 Identities = 67/83 (80%), Positives = 77/83 (92%)
 Frame = +1

Query: 4   PMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYKN 183
           P+GF+V+KNNYK+KL GEKSGRKGHL++ATEI+EVAPSL+MVELRKAGGDTLEFHKFYKN
Sbjct: 371 PLGFNVRKNNYKIKLQGEKSGRKGHLNVATEIYEVAPSLYMVELRKAGGDTLEFHKFYKN 430

Query: 184 LSTGLKDIVWKTGDEVKEEGNNG 252
           LSTGLKDIVW+ GDE  EE  +G
Sbjct: 431 LSTGLKDIVWQLGDEAGEEVKDG 453


>ref|XP_006389416.1| CBL-interacting protein kinase 23 [Populus trichocarpa]
           gi|116265966|gb|ABJ91231.1| CBL-interacting protein
           kinase 25 [Populus trichocarpa]
           gi|550312210|gb|ERP48330.1| CBL-interacting protein
           kinase 23 [Populus trichocarpa]
          Length = 466

 Score =  144 bits (364), Expect = 1e-32
 Identities = 69/86 (80%), Positives = 77/86 (89%)
 Frame = +1

Query: 1   APMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYK 180
           AP+GFDVKKNN+KMKL G+K GRKG LS+ATEIFEVAPSL+MVE+RK+GGDTLEFHKFYK
Sbjct: 380 APLGFDVKKNNFKMKLQGDKDGRKGRLSVATEIFEVAPSLYMVEVRKSGGDTLEFHKFYK 439

Query: 181 NLSTGLKDIVWKTGDEVKEEGNNGTD 258
           NLSTGLKDIVWKT DE KEE    T+
Sbjct: 440 NLSTGLKDIVWKTIDEEKEEEEAATN 465


>ref|XP_006415491.1| hypothetical protein EUTSA_v10007518mg [Eutrema salsugineum]
           gi|557093262|gb|ESQ33844.1| hypothetical protein
           EUTSA_v10007518mg [Eutrema salsugineum]
          Length = 477

 Score =  144 bits (363), Expect = 1e-32
 Identities = 69/87 (79%), Positives = 79/87 (90%)
 Frame = +1

Query: 4   PMGFDVKKNNYKMKLYGEKSGRKGHLSIATEIFEVAPSLHMVELRKAGGDTLEFHKFYKN 183
           PMGFDV+KNNYKMKL GEKSGRKG L++ TE+F+VAPSL+MVE+RK+GGDTLEFHKFYKN
Sbjct: 383 PMGFDVRKNNYKMKLLGEKSGRKGQLAVTTEVFQVAPSLYMVEMRKSGGDTLEFHKFYKN 442

Query: 184 LSTGLKDIVWKTGDEVKEEGNNGTDVS 264
           L+TGLKDIVWKT DE KEE   GT+VS
Sbjct: 443 LTTGLKDIVWKTIDEEKEE---GTEVS 466