BLASTX nr result
ID: Mentha22_contig00043003
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Mentha22_contig00043003
(945 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_007225337.1| hypothetical protein PRUPE_ppa001026mg [Prun... 346 9e-93
ref|XP_006365281.1| PREDICTED: putative E3 ubiquitin-protein lig... 342 1e-91
ref|XP_004296765.1| PREDICTED: putative E3 ubiquitin-protein lig... 338 1e-90
ref|XP_006489216.1| PREDICTED: putative E3 ubiquitin-protein lig... 338 2e-90
ref|XP_006489214.1| PREDICTED: putative E3 ubiquitin-protein lig... 338 2e-90
ref|XP_004229474.1| PREDICTED: putative E3 ubiquitin-protein lig... 338 2e-90
ref|XP_006419748.1| hypothetical protein CICLE_v10004269mg [Citr... 338 2e-90
ref|XP_007035384.1| RING/U-box superfamily protein, putative iso... 330 5e-88
ref|XP_007035383.1| RING/U-box superfamily protein, putative iso... 330 5e-88
ref|XP_002516824.1| conserved hypothetical protein [Ricinus comm... 322 1e-85
ref|XP_002277269.1| PREDICTED: putative E3 ubiquitin-protein lig... 320 4e-85
ref|XP_006597553.1| PREDICTED: putative E3 ubiquitin-protein lig... 305 2e-80
gb|EXB28629.1| Putative E3 ubiquitin-protein ligase RF298 [Morus... 300 6e-79
ref|XP_003542905.1| PREDICTED: putative E3 ubiquitin-protein lig... 297 5e-78
ref|XP_004159841.1| PREDICTED: LOW QUALITY PROTEIN: putative E3 ... 294 3e-77
ref|XP_004134170.1| PREDICTED: putative E3 ubiquitin-protein lig... 294 3e-77
ref|XP_007147828.1| hypothetical protein PHAVU_006G158500g [Phas... 292 1e-76
ref|XP_006584050.1| PREDICTED: putative E3 ubiquitin-protein lig... 285 1e-74
ref|XP_003528662.2| PREDICTED: putative E3 ubiquitin-protein lig... 285 1e-74
ref|XP_002312723.2| hypothetical protein POPTR_0008s20280g [Popu... 285 2e-74
>ref|XP_007225337.1| hypothetical protein PRUPE_ppa001026mg [Prunus persica]
gi|462422273|gb|EMJ26536.1| hypothetical protein
PRUPE_ppa001026mg [Prunus persica]
Length = 930
Score = 346 bits (887), Expect = 9e-93
Identities = 179/253 (70%), Positives = 202/253 (79%), Gaps = 4/253 (1%)
Frame = -2
Query: 797 MASMVAKACGTTSSQM-PVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFSA 621
MASMVAK + S+Q+ P +TVQEKGSRNKRKFRADPPL DPNK+IPLP ECTS+EFSA
Sbjct: 1 MASMVAKGTSSCSTQVSPSITVQEKGSRNKRKFRADPPLGDPNKIIPLPQTECTSYEFSA 60
Query: 620 EKYE-SQGH--INGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEFQ 450
EK+E +QGH I CD+C VN++ S TVG SE+G SRPREE EA DEFQ
Sbjct: 61 EKFEITQGHGQIGVCDLCTVNKDHSDGLKLDLGLSSTVGSSEVGPSRPREELEA--DEFQ 118
Query: 449 DADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVSN 270
DADWS+ TE++LEELVL+NL+ IF GY+EEVATKA+LRSGL YGCKD VSN
Sbjct: 119 DADWSDLTETQLEELVLSNLDTIFKSAIKKIVACGYAEEVATKAVLRSGLCYGCKDTVSN 178
Query: 269 IVDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLIC 90
IVDNTL FLRSG EID S+EH FEDL+Q+EKYILAELVC+LREVRPFFS GDAMWCLLIC
Sbjct: 179 IVDNTLNFLRSGQEIDPSREHCFEDLQQLEKYILAELVCVLREVRPFFSMGDAMWCLLIC 238
Query: 89 DMNVSHACAMDGD 51
DMNVSHACAMDGD
Sbjct: 239 DMNVSHACAMDGD 251
>ref|XP_006365281.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298-like [Solanum
tuberosum]
Length = 901
Score = 342 bits (877), Expect = 1e-91
Identities = 177/275 (64%), Positives = 206/275 (74%), Gaps = 10/275 (3%)
Frame = -2
Query: 797 MASMVAKACGTTSSQM-PVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFSA 621
MASMVAKAC T S+Q P +TV EKGSRNKRKFRADPPL DPNK+IP P ECTSFEFSA
Sbjct: 1 MASMVAKACATPSAQYTPAVTVLEKGSRNKRKFRADPPLVDPNKMIPSPQFECTSFEFSA 60
Query: 620 EKY---ESQGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEFQ 450
+K+ + NGCDMC + Q+ SC+VG SE+G S PRE E + ++F
Sbjct: 61 DKFGMIPTHELSNGCDMCSLKQDSSESLKLDLGLSCSVGSSEVGPSEPREVVETT-EQFH 119
Query: 449 DADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVSN 270
DADWS+FTE++LEELVL+NL+ IF GYSEE+ATKA+LRSG+ YGCKDIVSN
Sbjct: 120 DADWSDFTEAQLEELVLSNLDTIFRSAIKRIMAFGYSEEIATKAVLRSGICYGCKDIVSN 179
Query: 269 IVDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLIC 90
IV+NTL FLRSGH+ID S EHYFEDL QMEKY+LAELVC+LREVRPFFSTGDAMWCLLIC
Sbjct: 180 IVENTLVFLRSGHDIDSSGEHYFEDLLQMEKYVLAELVCVLREVRPFFSTGDAMWCLLIC 239
Query: 89 DMNVSHACAMDGDASSNM------NPSISAQSQLR 3
DMNVSHACAM+ D S++ N S S Q L+
Sbjct: 240 DMNVSHACAMESDPLSSLVGDGSENSSASVQPNLQ 274
>ref|XP_004296765.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298-like
[Fragaria vesca subsp. vesca]
Length = 888
Score = 338 bits (868), Expect = 1e-90
Identities = 176/275 (64%), Positives = 202/275 (73%), Gaps = 12/275 (4%)
Frame = -2
Query: 797 MASMVAKACGTTSSQMPVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFSAE 618
MASMVAK T+ P MTVQEKGSRNKRKFRADPPLADPNK++PLP EC +EFSA+
Sbjct: 1 MASMVAKGSSCTTQLSPSMTVQEKGSRNKRKFRADPPLADPNKILPLPQTECAGYEFSAD 60
Query: 617 KYE----SQGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEFQ 450
K+E + G + CD+C VNQ+ S VG SE+G SRPR E EA DEFQ
Sbjct: 61 KFEISHQNHGQTSVCDLCFVNQDHSDGLKLDLGLSSAVGSSEVGPSRPRRESEA--DEFQ 118
Query: 449 DADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVSN 270
DADWS+ TE++LEELVL+NL+ IF GY+E+VATKA+LRSGL YG KD VSN
Sbjct: 119 DADWSDLTETQLEELVLSNLDTIFKSAIKKIVACGYTEDVATKAVLRSGLCYGSKDTVSN 178
Query: 269 IVDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLIC 90
IVDNTL FLRSG EID S+EH FEDL+Q+EKYILAELVC+LRE+RPFFSTGDAMWCLLIC
Sbjct: 179 IVDNTLVFLRSGQEIDPSREHCFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLIC 238
Query: 89 DMNVSHACAMDG--------DASSNMNPSISAQSQ 9
DMNVSHACAMDG D +SN + IS Q Q
Sbjct: 239 DMNVSHACAMDGDPISSFLNDGTSNGSSPISNQPQ 273
>ref|XP_006489216.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298-like isoform
X3 [Citrus sinensis]
Length = 719
Score = 338 bits (867), Expect = 2e-90
Identities = 175/276 (63%), Positives = 203/276 (73%), Gaps = 11/276 (3%)
Frame = -2
Query: 797 MASMVAKACGTTSSQMPVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFSAE 618
MAS+VAK ++ P+M VQEKGSRNKRKFRADPPL +PNK+IP P NEC ++EF+AE
Sbjct: 1 MASLVAKGSSSSCQVSPLMLVQEKGSRNKRKFRADPPLGEPNKIIPSPQNECPTYEFTAE 60
Query: 617 KYE---SQGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEFQD 447
K++ G CD+C VNQ+ S VG SE+G SRPREE E V+EFQD
Sbjct: 61 KFDITPGHGQTGACDLCGVNQDHSDGLKLDLGLSSAVGSSEVGPSRPREELE--VEEFQD 118
Query: 446 ADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVSNI 267
ADWS+ TES+LEELVL+NL+AIF GY EEVATKA+LRSGL YG KD VSNI
Sbjct: 119 ADWSDLTESQLEELVLSNLDAIFKSAIKKIVACGYMEEVATKAVLRSGLCYGSKDTVSNI 178
Query: 266 VDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLICD 87
VDNTLAFLRSG EI+ S+EHYF+DL Q+EKYILAELVC+LREVRPFFSTGDAMWCLLICD
Sbjct: 179 VDNTLAFLRSGQEINSSREHYFQDLPQLEKYILAELVCVLREVRPFFSTGDAMWCLLICD 238
Query: 86 MNVSHACAMD--------GDASSNMNPSISAQSQLR 3
MNVSHACAMD GD +SN N I+ Q Q +
Sbjct: 239 MNVSHACAMDGDPLSSFSGDGASNGNSHITTQLQTK 274
>ref|XP_006489214.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298-like isoform
X1 [Citrus sinensis] gi|568872108|ref|XP_006489215.1|
PREDICTED: putative E3 ubiquitin-protein ligase
RF298-like isoform X2 [Citrus sinensis]
Length = 899
Score = 338 bits (867), Expect = 2e-90
Identities = 175/276 (63%), Positives = 203/276 (73%), Gaps = 11/276 (3%)
Frame = -2
Query: 797 MASMVAKACGTTSSQMPVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFSAE 618
MAS+VAK ++ P+M VQEKGSRNKRKFRADPPL +PNK+IP P NEC ++EF+AE
Sbjct: 1 MASLVAKGSSSSCQVSPLMLVQEKGSRNKRKFRADPPLGEPNKIIPSPQNECPTYEFTAE 60
Query: 617 KYE---SQGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEFQD 447
K++ G CD+C VNQ+ S VG SE+G SRPREE E V+EFQD
Sbjct: 61 KFDITPGHGQTGACDLCGVNQDHSDGLKLDLGLSSAVGSSEVGPSRPREELE--VEEFQD 118
Query: 446 ADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVSNI 267
ADWS+ TES+LEELVL+NL+AIF GY EEVATKA+LRSGL YG KD VSNI
Sbjct: 119 ADWSDLTESQLEELVLSNLDAIFKSAIKKIVACGYMEEVATKAVLRSGLCYGSKDTVSNI 178
Query: 266 VDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLICD 87
VDNTLAFLRSG EI+ S+EHYF+DL Q+EKYILAELVC+LREVRPFFSTGDAMWCLLICD
Sbjct: 179 VDNTLAFLRSGQEINSSREHYFQDLPQLEKYILAELVCVLREVRPFFSTGDAMWCLLICD 238
Query: 86 MNVSHACAMD--------GDASSNMNPSISAQSQLR 3
MNVSHACAMD GD +SN N I+ Q Q +
Sbjct: 239 MNVSHACAMDGDPLSSFSGDGASNGNSHITTQLQTK 274
>ref|XP_004229474.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298-like [Solanum
lycopersicum]
Length = 901
Score = 338 bits (867), Expect = 2e-90
Identities = 176/275 (64%), Positives = 204/275 (74%), Gaps = 10/275 (3%)
Frame = -2
Query: 797 MASMVAKACGTTSSQM-PVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFSA 621
MASMVAKAC T S+Q P +TV EKGSRNKRKFRADPPL DPNK+I P ECTSFEFSA
Sbjct: 1 MASMVAKACATPSAQYTPAVTVLEKGSRNKRKFRADPPLVDPNKMISSPQFECTSFEFSA 60
Query: 620 EKY---ESQGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEFQ 450
+K+ + NGCDMC + Q+ SC+VG SE+G S PRE E + ++F
Sbjct: 61 DKFGMIPTHELSNGCDMCSLKQDSSESLKLDLGLSCSVGSSEVGPSEPREVVETT-EQFH 119
Query: 449 DADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVSN 270
DADWS+FTE++LEELVL NL+ IF GYSEE+ATKA+LRSG+ YGCKDIVSN
Sbjct: 120 DADWSDFTEAQLEELVLNNLDTIFRSAIKRIMAFGYSEEIATKAVLRSGICYGCKDIVSN 179
Query: 269 IVDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLIC 90
IV+NTL FLRSGH+ID S EHYFEDL QMEKY+LAELVC+LREVRPFFSTGDAMWCLLIC
Sbjct: 180 IVENTLVFLRSGHDIDSSGEHYFEDLLQMEKYVLAELVCVLREVRPFFSTGDAMWCLLIC 239
Query: 89 DMNVSHACAMDGDASSNM------NPSISAQSQLR 3
DMNVSHACAM+ D S++ N S S Q L+
Sbjct: 240 DMNVSHACAMESDPLSSLVVDSSENSSASLQPHLQ 274
>ref|XP_006419748.1| hypothetical protein CICLE_v10004269mg [Citrus clementina]
gi|567853171|ref|XP_006419749.1| hypothetical protein
CICLE_v10004269mg [Citrus clementina]
gi|567853173|ref|XP_006419750.1| hypothetical protein
CICLE_v10004269mg [Citrus clementina]
gi|557521621|gb|ESR32988.1| hypothetical protein
CICLE_v10004269mg [Citrus clementina]
gi|557521622|gb|ESR32989.1| hypothetical protein
CICLE_v10004269mg [Citrus clementina]
gi|557521623|gb|ESR32990.1| hypothetical protein
CICLE_v10004269mg [Citrus clementina]
Length = 900
Score = 338 bits (866), Expect = 2e-90
Identities = 176/277 (63%), Positives = 207/277 (74%), Gaps = 12/277 (4%)
Frame = -2
Query: 797 MASMVAKACGTTSSQM-PVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFSA 621
MAS+VAK ++S Q+ P+M VQEKGSRNKRKFRADPPL +PNK+IP P NEC ++EF+A
Sbjct: 1 MASLVAKGSSSSSCQVSPLMLVQEKGSRNKRKFRADPPLGEPNKIIPSPQNECPTYEFTA 60
Query: 620 EKYE---SQGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEFQ 450
EK++ G CD+C VNQ+ S VG SE+G S+PREE E V+EFQ
Sbjct: 61 EKFDITPGHGQTGACDLCGVNQDHSDGLKLDLGLSSAVGSSEVGPSQPREELE--VEEFQ 118
Query: 449 DADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVSN 270
DADWS+ TES+LEELVL+NL+AIF GY+EEVATKA+LRSGL YG KD VSN
Sbjct: 119 DADWSDLTESQLEELVLSNLDAIFKSAIKKIVACGYTEEVATKAVLRSGLCYGSKDTVSN 178
Query: 269 IVDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLIC 90
IVDNTLAFLRSG EI+ S+EHYF+DL Q+EKYILAELVC+LREVRPFFSTGDAMWCLLIC
Sbjct: 179 IVDNTLAFLRSGQEINSSREHYFQDLPQLEKYILAELVCVLREVRPFFSTGDAMWCLLIC 238
Query: 89 DMNVSHACAMD--------GDASSNMNPSISAQSQLR 3
DMNVSHACAMD GD +SN N I+ Q Q +
Sbjct: 239 DMNVSHACAMDGDPLSSFSGDGASNGNSHITTQLQTK 275
>ref|XP_007035384.1| RING/U-box superfamily protein, putative isoform 2 [Theobroma
cacao] gi|590660376|ref|XP_007035385.1| RING/U-box
superfamily protein, putative isoform 2 [Theobroma
cacao] gi|508714413|gb|EOY06310.1| RING/U-box
superfamily protein, putative isoform 2 [Theobroma
cacao] gi|508714414|gb|EOY06311.1| RING/U-box
superfamily protein, putative isoform 2 [Theobroma
cacao]
Length = 737
Score = 330 bits (846), Expect = 5e-88
Identities = 170/256 (66%), Positives = 201/256 (78%), Gaps = 4/256 (1%)
Frame = -2
Query: 797 MASMVAKACGTTSSQM-PVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFSA 621
MASMV G++SSQ+ P++++QEKGSRNKRKFRADPPL DPNK+IP P NE S+EF A
Sbjct: 1 MASMVLN--GSSSSQVSPLISIQEKGSRNKRKFRADPPLGDPNKIIPSPQNEYPSYEFCA 58
Query: 620 EKYES---QGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEFQ 450
EK+E G + CD+C VNQ+ S TVG SE+G S+PREE EA DE+Q
Sbjct: 59 EKFEITPVHGQASACDLCGVNQDHSDGLKLDLGLSSTVGSSEVGPSQPREEIEA--DEYQ 116
Query: 449 DADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVSN 270
DADWS+ TES+LEELVL+NL+AIF GY+EE+ATKA+LRSGL YGCKD VSN
Sbjct: 117 DADWSDLTESQLEELVLSNLDAIFKSAIKKIVACGYTEEIATKAVLRSGLCYGCKDTVSN 176
Query: 269 IVDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLIC 90
IVDNTLAFLRSG +I+ S++H FEDL+Q+EKYILAELVC+LREVRPFFSTGDAMWCLLIC
Sbjct: 177 IVDNTLAFLRSGQDINSSRDHCFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLIC 236
Query: 89 DMNVSHACAMDGDASS 42
DMNVSHAC+MDGD S
Sbjct: 237 DMNVSHACSMDGDPLS 252
>ref|XP_007035383.1| RING/U-box superfamily protein, putative isoform 1 [Theobroma
cacao] gi|590660380|ref|XP_007035386.1| RING/U-box
superfamily protein, putative isoform 1 [Theobroma
cacao] gi|590660383|ref|XP_007035387.1| RING/U-box
superfamily protein, putative isoform 1 [Theobroma
cacao] gi|508714412|gb|EOY06309.1| RING/U-box
superfamily protein, putative isoform 1 [Theobroma
cacao] gi|508714415|gb|EOY06312.1| RING/U-box
superfamily protein, putative isoform 1 [Theobroma
cacao] gi|508714416|gb|EOY06313.1| RING/U-box
superfamily protein, putative isoform 1 [Theobroma
cacao]
Length = 893
Score = 330 bits (846), Expect = 5e-88
Identities = 170/256 (66%), Positives = 201/256 (78%), Gaps = 4/256 (1%)
Frame = -2
Query: 797 MASMVAKACGTTSSQM-PVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFSA 621
MASMV G++SSQ+ P++++QEKGSRNKRKFRADPPL DPNK+IP P NE S+EF A
Sbjct: 1 MASMVLN--GSSSSQVSPLISIQEKGSRNKRKFRADPPLGDPNKIIPSPQNEYPSYEFCA 58
Query: 620 EKYES---QGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEFQ 450
EK+E G + CD+C VNQ+ S TVG SE+G S+PREE EA DE+Q
Sbjct: 59 EKFEITPVHGQASACDLCGVNQDHSDGLKLDLGLSSTVGSSEVGPSQPREEIEA--DEYQ 116
Query: 449 DADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVSN 270
DADWS+ TES+LEELVL+NL+AIF GY+EE+ATKA+LRSGL YGCKD VSN
Sbjct: 117 DADWSDLTESQLEELVLSNLDAIFKSAIKKIVACGYTEEIATKAVLRSGLCYGCKDTVSN 176
Query: 269 IVDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLIC 90
IVDNTLAFLRSG +I+ S++H FEDL+Q+EKYILAELVC+LREVRPFFSTGDAMWCLLIC
Sbjct: 177 IVDNTLAFLRSGQDINSSRDHCFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLIC 236
Query: 89 DMNVSHACAMDGDASS 42
DMNVSHAC+MDGD S
Sbjct: 237 DMNVSHACSMDGDPLS 252
>ref|XP_002516824.1| conserved hypothetical protein [Ricinus communis]
gi|223543912|gb|EEF45438.1| conserved hypothetical
protein [Ricinus communis]
Length = 894
Score = 322 bits (826), Expect = 1e-85
Identities = 171/277 (61%), Positives = 203/277 (73%), Gaps = 13/277 (4%)
Frame = -2
Query: 797 MASMVAKACGTTSSQM--PVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFS 624
MASMVAKA ++ S +++VQEKGSRNKRKFRAD PL DP K+IP P NEC+ +EFS
Sbjct: 1 MASMVAKASSSSCSTQVSSLVSVQEKGSRNKRKFRADTPLGDPGKIIPSPQNECSGYEFS 60
Query: 623 AEKYES---QGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEF 453
AEK+E+ G + CD+C VNQ+ S + SE+G S+PREE E+ +E
Sbjct: 61 AEKFEATPAHGPSSVCDLCGVNQDHSEGLKLDLGLSSALSSSEVGTSQPREELES--EES 118
Query: 452 QDADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVS 273
DADWS+ TES+LEELVL+NL+AIF GY+EEVATKA+LRSGL YGCKD VS
Sbjct: 119 HDADWSDLTESQLEELVLSNLDAIFKSAIKKIVACGYTEEVATKAVLRSGLCYGCKDTVS 178
Query: 272 NIVDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLI 93
NIVDNTLAFLR+G EID S++H FEDL+Q+EKYILAELVC+LREVRPFFSTGDAMWCLLI
Sbjct: 179 NIVDNTLAFLRNGQEIDPSRDHCFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLI 238
Query: 92 CDMNVSHACAMD--------GDASSNMNPSISAQSQL 6
CDMNVSHACAMD GD +SN S S Q Q+
Sbjct: 239 CDMNVSHACAMDGDPLSGFAGDGTSNGTSSTSNQPQI 275
>ref|XP_002277269.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298-like [Vitis
vinifera]
Length = 893
Score = 320 bits (821), Expect = 4e-85
Identities = 165/257 (64%), Positives = 194/257 (75%), Gaps = 3/257 (1%)
Frame = -2
Query: 797 MASMVAKACGTTSSQMPVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFSAE 618
MA + CGT S P ++ QEKGSRNKRKFRADPPL DPNK++ ++C S+EFSAE
Sbjct: 4 MAVNGSSTCGTQGS--PSVSAQEKGSRNKRKFRADPPLGDPNKIVS-SQDQCLSYEFSAE 60
Query: 617 KYE---SQGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEFQD 447
K+E S G C MC +NQ+ S G SE+G S+PR+E EA D+FQD
Sbjct: 61 KFEVTSSHGQPGACGMCNLNQDHSDGLKLDLGLSSAAGSSEVGPSQPRDELEA--DDFQD 118
Query: 446 ADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVSNI 267
ADWS+ TES+LEELVL+NL+ IF GYSEEVATKA+LRSGL YGCKD VSNI
Sbjct: 119 ADWSDLTESQLEELVLSNLDTIFKSAIKKIVACGYSEEVATKAVLRSGLCYGCKDTVSNI 178
Query: 266 VDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLICD 87
VDNTLAFLR+G EID S+EHYF+DL+Q+EKYILAELVC+LREVRPFFSTGDAMWCLLICD
Sbjct: 179 VDNTLAFLRNGQEIDPSREHYFDDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICD 238
Query: 86 MNVSHACAMDGDASSNM 36
MNVSHACAMDGD+ S++
Sbjct: 239 MNVSHACAMDGDSFSSI 255
>ref|XP_006597553.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298-like [Glycine
max]
Length = 885
Score = 305 bits (780), Expect = 2e-80
Identities = 161/258 (62%), Positives = 193/258 (74%), Gaps = 4/258 (1%)
Frame = -2
Query: 797 MASMVAKACGTTSSQM-PVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFSA 621
MAS+VA SSQM P ++VQEKGSRNKRKFRADPPL +PNK+IPLP +E S+EFSA
Sbjct: 1 MASLVASG----SSQMAPSVSVQEKGSRNKRKFRADPPLGEPNKIIPLPQHESLSYEFSA 56
Query: 620 EKYE---SQGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEFQ 450
EK+E G ++ MC V+Q+ S V S++ +S+P+EE E VDEF
Sbjct: 57 EKFEITPGHGQVSASGMCSVSQDHSDALKLDLGLSSPVASSDVRISQPKEELE--VDEFH 114
Query: 449 DADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVSN 270
DADWS+ TE++LEELVL+NL+ IF GY E+VATKAILRSG+ YGCKD VSN
Sbjct: 115 DADWSDLTEAQLEELVLSNLDTIFKSAIKKIVACGYIEDVATKAILRSGICYGCKDAVSN 174
Query: 269 IVDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLIC 90
+VDN LAFLR+G EI+ S+EHYFEDL Q+EKYILAELVC+LREVRP FSTGDAMW LLIC
Sbjct: 175 VVDNGLAFLRNGQEINPSREHYFEDLVQLEKYILAELVCVLREVRPLFSTGDAMWRLLIC 234
Query: 89 DMNVSHACAMDGDASSNM 36
DMNVS ACAMDGD SS++
Sbjct: 235 DMNVSLACAMDGDPSSSL 252
>gb|EXB28629.1| Putative E3 ubiquitin-protein ligase RF298 [Morus notabilis]
Length = 728
Score = 300 bits (768), Expect = 6e-79
Identities = 161/271 (59%), Positives = 193/271 (71%), Gaps = 8/271 (2%)
Frame = -2
Query: 797 MASMVAKACGTTSSQMPV---MTVQEKGSRNKRKFRADPPLADPNKVIPLPS--NECTSF 633
MASMVA A SS+ V +TVQ+KGSRNKRKFRADPPL D NK++ LP ++ +S+
Sbjct: 1 MASMVAPASSGCSSRSQVSSSLTVQQKGSRNKRKFRADPPLGDMNKIVSLPQTQSDSSSY 60
Query: 632 EFSAEKYE---SQGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASV 462
FSAEK+E Q N CD+C VN S +VG SE+G SRPREE EA
Sbjct: 61 VFSAEKFEINPGQPQSNVCDLCSVNLNHSDNLKLDLGLSSSVGTSEVGPSRPREEVEA-- 118
Query: 461 DEFQDADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKD 282
DEF DADWS+ TE++LEELV++NL+ IF GY+EEVATKA+LRSG YG KD
Sbjct: 119 DEFHDADWSDLTEAQLEELVISNLDTIFRSAIKKFVACGYTEEVATKAVLRSGHCYGSKD 178
Query: 281 IVSNIVDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWC 102
VSNIVD+TLAFLRSG EID S+EH F+DL+Q+E+Y+LAELVC+LREVRPFFSTGDAMWC
Sbjct: 179 TVSNIVDSTLAFLRSGQEIDPSREHCFDDLRQLEEYVLAELVCVLREVRPFFSTGDAMWC 238
Query: 101 LLICDMNVSHACAMDGDASSNMNPSISAQSQ 9
LLICDMNVSHACAMD + + A +
Sbjct: 239 LLICDMNVSHACAMDASLKISKARGVDAPQE 269
>ref|XP_003542905.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298-like [Glycine
max]
Length = 883
Score = 297 bits (760), Expect = 5e-78
Identities = 160/258 (62%), Positives = 189/258 (73%), Gaps = 4/258 (1%)
Frame = -2
Query: 797 MASMVAKACGTTSSQM-PVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFSA 621
MAS+VA SSQM P ++VQEKGSRNKRKFRADPPL +PNK+IP P +E S EFSA
Sbjct: 1 MASLVASG----SSQMAPSVSVQEKGSRNKRKFRADPPLGEPNKIIPSPQHESLSNEFSA 56
Query: 620 EKYE---SQGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEFQ 450
EK+E G + DMC V+Q+ S + S++ LS+P+EE E VDEF
Sbjct: 57 EKFEITTGHGQASASDMCSVSQDHSDGLKLDLGLSSPLPSSDVRLSQPKEELE--VDEFH 114
Query: 449 DADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVSN 270
DADWS+ TE++LEELVL+NL+ IF GY E+VATKAILRSG+ YGCKD VSN
Sbjct: 115 DADWSDLTEAQLEELVLSNLDTIFKSAVKKIVACGYIEDVATKAILRSGICYGCKDAVSN 174
Query: 269 IVDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLIC 90
+VD LAFLR+G EID S+EHYFEDL Q+EKYILAELVC+LREVRP FSTGDAMW LLIC
Sbjct: 175 VVDKGLAFLRNGQEIDPSREHYFEDLVQLEKYILAELVCVLREVRPLFSTGDAMWRLLIC 234
Query: 89 DMNVSHACAMDGDASSNM 36
DMNVS ACAMD D SS++
Sbjct: 235 DMNVSLACAMDDDPSSSL 252
>ref|XP_004159841.1| PREDICTED: LOW QUALITY PROTEIN: putative E3 ubiquitin-protein
ligase RF298-like [Cucumis sativus]
Length = 901
Score = 294 bits (753), Expect = 3e-77
Identities = 162/279 (58%), Positives = 198/279 (70%), Gaps = 18/279 (6%)
Frame = -2
Query: 797 MASMVAK-ACGTTSSQMPV-MTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFS 624
MASMVAK +C +TS+ P MTVQEKGSRNKRK+RADPPL D NK+ ++C S+EFS
Sbjct: 1 MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60
Query: 623 AEKYE---SQGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEF 453
AEK+E S G +GCD+C ++QE S G S++G++ PR E E VDE
Sbjct: 61 AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLS-NGGSSDVGINWPRGELE--VDED 117
Query: 452 QDADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVS 273
QDADWS+ TE++LEELVL NL+ IF SGY+EEVA KA+ RSG+ +G KD VS
Sbjct: 118 QDADWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVS 177
Query: 272 NIVDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLI 93
N+VDNTLAFLR G EIDHS+EHYFEDL+Q+EKYILAELVC+LRE+RPFFSTGDAMWCLLI
Sbjct: 178 NVVDNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLI 237
Query: 92 CDMNVSHACAMDGD-------------ASSNMNPSISAQ 15
DM+V+ ACAMD D +SSN P + A+
Sbjct: 238 SDMSVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAE 276
>ref|XP_004134170.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298-like [Cucumis
sativus]
Length = 901
Score = 294 bits (753), Expect = 3e-77
Identities = 162/279 (58%), Positives = 198/279 (70%), Gaps = 18/279 (6%)
Frame = -2
Query: 797 MASMVAK-ACGTTSSQMPV-MTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFS 624
MASMVAK +C +TS+ P MTVQEKGSRNKRK+RADPPL D NK+ ++C S+EFS
Sbjct: 1 MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60
Query: 623 AEKYE---SQGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEF 453
AEK+E S G +GCD+C ++QE S G S++G++ PR E E VDE
Sbjct: 61 AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLS-NGGSSDVGINWPRGELE--VDED 117
Query: 452 QDADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVS 273
QDADWS+ TE++LEELVL NL+ IF SGY+EEVA KA+ RSG+ +G KD VS
Sbjct: 118 QDADWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVS 177
Query: 272 NIVDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLI 93
N+VDNTLAFLR G EIDHS+EHYFEDL+Q+EKYILAELVC+LRE+RPFFSTGDAMWCLLI
Sbjct: 178 NVVDNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLI 237
Query: 92 CDMNVSHACAMDGD-------------ASSNMNPSISAQ 15
DM+V+ ACAMD D +SSN P + A+
Sbjct: 238 SDMSVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAE 276
>ref|XP_007147828.1| hypothetical protein PHAVU_006G158500g [Phaseolus vulgaris]
gi|593694616|ref|XP_007147829.1| hypothetical protein
PHAVU_006G158500g [Phaseolus vulgaris]
gi|561021051|gb|ESW19822.1| hypothetical protein
PHAVU_006G158500g [Phaseolus vulgaris]
gi|561021052|gb|ESW19823.1| hypothetical protein
PHAVU_006G158500g [Phaseolus vulgaris]
Length = 884
Score = 292 bits (748), Expect = 1e-76
Identities = 158/270 (58%), Positives = 191/270 (70%), Gaps = 7/270 (2%)
Frame = -2
Query: 797 MASMVAKACGTTSSQMPVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFSAE 618
MAS+VA G +S P ++VQEKGSRNKRKFRADPPL +PNK IP +E S+EFSAE
Sbjct: 1 MASLVA---GASSRTTPSVSVQEKGSRNKRKFRADPPLGEPNKSIPSVQHESLSYEFSAE 57
Query: 617 KYE---SQGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEFQD 447
K+E G ++ DMC VNQ+ S V S+ LS+P+EE E DEF D
Sbjct: 58 KFEITPGHGQVSTSDMCSVNQDHSDGLKLGLGLSSPVVSSDFRLSQPKEESEVD-DEFHD 116
Query: 446 ADWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVSNI 267
ADWS+ TE+ELEEL++++L IF GY+E+VATKAILRSG+ YGCKD VSN+
Sbjct: 117 ADWSDLTEAELEELLMSSLNIIFKSAIKKMVACGYTEDVATKAILRSGICYGCKDAVSNV 176
Query: 266 VDNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLICD 87
VDN LAFLR+G E D S+EHYFEDL Q+EKYILAELVC+LREVRPF+S GDAMW LLI D
Sbjct: 177 VDNGLAFLRNGQE-DPSREHYFEDLMQLEKYILAELVCVLREVRPFYSIGDAMWALLISD 235
Query: 86 MNVSHACAMDGDASSNMN----PSISAQSQ 9
MNVSHACAMDGD S++ S+ +SQ
Sbjct: 236 MNVSHACAMDGDPSNSFGSDGCSSVQTESQ 265
>ref|XP_006584050.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298-like isoform
X2 [Glycine max]
Length = 726
Score = 285 bits (730), Expect = 1e-74
Identities = 149/256 (58%), Positives = 179/256 (69%), Gaps = 10/256 (3%)
Frame = -2
Query: 749 PVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFSAEKYE---SQGHINGCDM 579
P ++ QEKGSRNKRKFR DPPL +PNK IP P +C S+EFSAE++E G CD+
Sbjct: 3 PSVSCQEKGSRNKRKFRVDPPLGEPNKFIPAPQLKCFSYEFSAERFEITPGHGQAAACDL 62
Query: 578 CCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEFQDADWSEFTESELEELVL 399
C V+Q+ G SE+G S+ ++E E DE DADWS+ TE++LEELVL
Sbjct: 63 CGVSQDYSDGLKLGLGLY-NPGTSEVGPSQSKDEPET--DEINDADWSDLTEAQLEELVL 119
Query: 398 TNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVSNIVDNTLAFLRSGHEIDH 219
TNL+ I GY+E+VATKAILR G+ YGCKD +SNIVDN+LAFLR+G EID
Sbjct: 120 TNLDTILKSAIKKIVACGYTEDVATKAILRPGICYGCKDTLSNIVDNSLAFLRNGQEIDT 179
Query: 218 SKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLICDMNVSHACAMD------ 57
S+EHYFEDL Q+EKY LAELVC+LREVRPFFS GDAMWCLLICDMNVSHACAMD
Sbjct: 180 SREHYFEDLVQLEKYNLAELVCVLREVRPFFSVGDAMWCLLICDMNVSHACAMDCNPLSS 239
Query: 56 -GDASSNMNPSISAQS 12
G+ +S PS A+S
Sbjct: 240 LGNDNSTGGPSNQAES 255
>ref|XP_003528662.2| PREDICTED: putative E3 ubiquitin-protein ligase RF298-like isoform
X1 [Glycine max]
Length = 879
Score = 285 bits (730), Expect = 1e-74
Identities = 149/256 (58%), Positives = 179/256 (69%), Gaps = 10/256 (3%)
Frame = -2
Query: 749 PVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFSAEKYE---SQGHINGCDM 579
P ++ QEKGSRNKRKFR DPPL +PNK IP P +C S+EFSAE++E G CD+
Sbjct: 3 PSVSCQEKGSRNKRKFRVDPPLGEPNKFIPAPQLKCFSYEFSAERFEITPGHGQAAACDL 62
Query: 578 CCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEFQDADWSEFTESELEELVL 399
C V+Q+ G SE+G S+ ++E E DE DADWS+ TE++LEELVL
Sbjct: 63 CGVSQDYSDGLKLGLGLY-NPGTSEVGPSQSKDEPET--DEINDADWSDLTEAQLEELVL 119
Query: 398 TNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVSNIVDNTLAFLRSGHEIDH 219
TNL+ I GY+E+VATKAILR G+ YGCKD +SNIVDN+LAFLR+G EID
Sbjct: 120 TNLDTILKSAIKKIVACGYTEDVATKAILRPGICYGCKDTLSNIVDNSLAFLRNGQEIDT 179
Query: 218 SKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLICDMNVSHACAMD------ 57
S+EHYFEDL Q+EKY LAELVC+LREVRPFFS GDAMWCLLICDMNVSHACAMD
Sbjct: 180 SREHYFEDLVQLEKYNLAELVCVLREVRPFFSVGDAMWCLLICDMNVSHACAMDCNPLSS 239
Query: 56 -GDASSNMNPSISAQS 12
G+ +S PS A+S
Sbjct: 240 LGNDNSTGGPSNQAES 255
>ref|XP_002312723.2| hypothetical protein POPTR_0008s20280g [Populus trichocarpa]
gi|550333523|gb|EEE90090.2| hypothetical protein
POPTR_0008s20280g [Populus trichocarpa]
Length = 781
Score = 285 bits (728), Expect = 2e-74
Identities = 150/255 (58%), Positives = 181/255 (70%), Gaps = 2/255 (0%)
Frame = -2
Query: 797 MASMVAKACGTTS--SQMPVMTVQEKGSRNKRKFRADPPLADPNKVIPLPSNECTSFEFS 624
MASMVAKA ++ +P+ ++QEKGSRNKRKFRADPPL DP+K++ NEC +EFS
Sbjct: 1 MASMVAKASSSSCPIQVLPMASIQEKGSRNKRKFRADPPLGDPSKIMSSAQNECPGYEFS 60
Query: 623 AEKYESQGHINGCDMCCVNQEXXXXXXXXXXXSCTVGPSEIGLSRPREEREASVDEFQDA 444
AEK+E+ G SE+G S+PR E E+ +E DA
Sbjct: 61 AEKFEA----------------------------APGSSEVGPSQPRGEVES--EESHDA 90
Query: 443 DWSEFTESELEELVLTNLEAIFXXXXXXXXXSGYSEEVATKAILRSGLWYGCKDIVSNIV 264
DWS+ TES+LEELVL+NL+AIF GY+EE ATKAILRSGL YGCK VSNIV
Sbjct: 91 DWSDLTESQLEELVLSNLDAIFKGAIKKIVACGYTEEEATKAILRSGLCYGCKYTVSNIV 150
Query: 263 DNTLAFLRSGHEIDHSKEHYFEDLKQMEKYILAELVCLLREVRPFFSTGDAMWCLLICDM 84
DNTLA LR+GH+I+ S+EH FEDL+Q+ +Y+LAELVC+LREVRPFFSTGDAMWCLLICDM
Sbjct: 151 DNTLALLRNGHDIEPSREHCFEDLQQLGRYVLAELVCVLREVRPFFSTGDAMWCLLICDM 210
Query: 83 NVSHACAMDGDASSN 39
NVSHACAMDGD S+
Sbjct: 211 NVSHACAMDGDPLSS 225