BLASTX nr result
ID: Mentha22_contig00039922
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Mentha22_contig00039922
(1287 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EYU33286.1| hypothetical protein MIMGU_mgv1a001869mg [Mimulus... 415 e-113
ref|XP_006355876.1| PREDICTED: isoamylase 2, chloroplastic [Sola... 409 e-111
ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] gi|... 409 e-111
ref|XP_004247144.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2... 397 e-108
ref|XP_007201750.1| hypothetical protein PRUPE_ppa001199mg [Prun... 370 e-100
ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like ... 368 4e-99
ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2... 367 5e-99
ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic-like ... 367 6e-99
gb|EXB39337.1| Isoamylase 2 [Morus notabilis] 363 9e-98
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2... 361 4e-97
ref|XP_006479763.1| PREDICTED: isoamylase 2, chloroplastic-like ... 355 2e-95
ref|XP_006444132.1| hypothetical protein CICLE_v10023436mg [Citr... 353 7e-95
ref|XP_007050677.1| Debranching enzyme 1 [Theobroma cacao] gi|50... 352 2e-94
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti... 352 2e-94
ref|XP_006386822.1| hypothetical protein POPTR_0002s22530g [Popu... 349 1e-93
gb|AAY89379.1| isoamylase isoform 2 [Nicotiana langsdorffii x Ni... 345 2e-92
ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like ... 328 2e-87
ref|XP_002889452.1| ATISA2/BE2/DBE1/ISA2 [Arabidopsis lyrata sub... 328 4e-87
gb|AAM98123.1| putative isoamylase [Arabidopsis thaliana] 326 2e-86
ref|NP_171830.1| Isoamylase 2 [Arabidopsis thaliana] gi|42571321... 326 2e-86
>gb|EYU33286.1| hypothetical protein MIMGU_mgv1a001869mg [Mimulus guttatus]
Length = 747
Score = 415 bits (1067), Expect = e-113
Identities = 219/349 (62%), Positives = 254/349 (72%), Gaps = 6/349 (1%)
Frame = +2
Query: 257 EFGDRLRVVVEKKYNNKYGVHVELESLQFFGGVEEPVMIWGLFISNSTSLMQLDFQQSSV 436
E G +L+V+V+K NNKYGV+VE+ESLQ E +MIWG+F + T M + QQSS
Sbjct: 2 EIGGQLKVMVKKNNNNKYGVYVEVESLQLDEEQRELIMIWGIFRDDLTKFMPV--QQSST 59
Query: 437 DGQCETRFVGG----AVELEFEADLAPFYVXXXXXXXXXXXXXXXTIRSHRNTNFTVPVG 604
DG ET FV +VELEFE LAPFYV TIRSHR TNF VPVG
Sbjct: 60 DGTTETPFVKDCDRLSVELEFEESLAPFYVSILLKSKLGSNSKTSTIRSHRKTNFIVPVG 119
Query: 605 FGAGHPSPLGVSFSNNGSVNFAIVSCDAESVVLCLYADATSEEPSLEIDLDPYINRSGNI 784
F +G PSPLG+S N+G +NFA S +A SVVLCLYAD T ++P+LEIDLDPY+NRSG+I
Sbjct: 120 FRSGRPSPLGISLLNDGFINFAFFSRNAYSVVLCLYADTTVDKPALEIDLDPYVNRSGDI 179
Query: 785 WHALL-YSPMPFVSYGYRCRSG-VENKDNRVLLDPYAKVIGVSGLNQPRKWLGNLCEEPV 958
WHA + S +PFVSYGYRCRS ++K +RVL DPYAKVI G PRK LG LC E
Sbjct: 180 WHASIDCSSLPFVSYGYRCRSSSTDSKGHRVLPDPYAKVIEEFG---PRKCLGRLCNESA 236
Query: 959 FDWSGEVRPNLPMEKMIVYRLNVGRFTKDKSSKLADGVAGSFSAISEKLQHFKDLGVNAI 1138
FDWSGE RPNLPMEK+IVYRLNV RFT DKSSKL +AGSFS IS+KL +FK+LGVNA+
Sbjct: 237 FDWSGESRPNLPMEKLIVYRLNVARFTNDKSSKLPRDIAGSFSGISDKLHYFKNLGVNAV 296
Query: 1139 LLEPVFPFGEQNGPYFPLHFFSPDSLYGPSRDAVNVANSMKDMVKKLHA 1285
LLEP+FPF EQ GPYFP HFFSP SLYGPS D VANS+K+MVKKLHA
Sbjct: 297 LLEPIFPFDEQKGPYFPWHFFSPGSLYGPSGDPSKVANSLKEMVKKLHA 345
>ref|XP_006355876.1| PREDICTED: isoamylase 2, chloroplastic [Solanum tuberosum]
Length = 878
Score = 409 bits (1052), Expect = e-111
Identities = 213/384 (55%), Positives = 266/384 (69%), Gaps = 13/384 (3%)
Frame = +2
Query: 173 SQKLKAVATPDVSVVKTSEKVATYRFRTEFGDRLRVVVEKKYNNKYGVHVELESLQFFGG 352
+ + KA++ +S+V ++++V TY FRT+ G +++V+VE+ N KY V VE+ L+
Sbjct: 65 AHRRKALSASRISLVPSAKRVPTYLFRTDIGGQVKVLVERT-NGKYKVLVEVLPLELSYA 123
Query: 353 VEEPVMIWGLFISNSTSLMQLDFQQSSVDGQC---ETRFVGG-----AVELEFEADLAPF 508
E VM+WGLF S+++ M LD + DG+ ET FV G VEL+FEA LAPF
Sbjct: 124 HSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVELDFEASLAPF 183
Query: 509 YVXXXXXXXXXXXXXXXTIRSHRNTNFTVPVGFGAGHPSPLGVSFSNNGSVNFAIVSCDA 688
Y+ IRSHRNTNF VPVG +GHP+PLG+SF +GSVNFA+ S A
Sbjct: 184 YISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGSVNFALFSRSA 243
Query: 689 ESVVLCLYADATSEEPSLEIDLDPYINRSGNIWHALLYSPMPFVSYGYRCRSGVENKDNR 868
SVVLCLY D + E+PSLEIDLDPYINRSG+IWHA L +PF +YGYRC++ K
Sbjct: 244 RSVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDCSLPFKTYGYRCKATTSGKGEL 303
Query: 869 VLLDPYAKVIG-----VSGLNQPRKWLGNLCEEPVFDWSGEVRPNLPMEKMIVYRLNVGR 1033
VLLDPYAKVI G K+LG LC EP +DWSG+V P+LPMEK+I+YRLNV +
Sbjct: 304 VLLDPYAKVIRRVIPRQGGSEIRPKYLGELCLEPGYDWSGDVPPSLPMEKLIIYRLNVTQ 363
Query: 1034 FTKDKSSKLADGVAGSFSAISEKLQHFKDLGVNAILLEPVFPFGEQNGPYFPLHFFSPDS 1213
FTKDKSSKL D +AG+FS ISEK HFKDLGVNA+LLEP+FPF EQ GPYFP HFFSP +
Sbjct: 364 FTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNAMLLEPIFPFDEQKGPYFPWHFFSPGN 423
Query: 1214 LYGPSRDAVNVANSMKDMVKKLHA 1285
+YGPS D ++ SMKDMVKKLHA
Sbjct: 424 MYGPSGDPLSAIKSMKDMVKKLHA 447
>ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] gi|27728147|gb|AAN15318.1|
isoamylase isoform 2 [Solanum tuberosum]
Length = 878
Score = 409 bits (1052), Expect = e-111
Identities = 213/384 (55%), Positives = 266/384 (69%), Gaps = 13/384 (3%)
Frame = +2
Query: 173 SQKLKAVATPDVSVVKTSEKVATYRFRTEFGDRLRVVVEKKYNNKYGVHVELESLQFFGG 352
+ + KA++ +S+V ++++V TY FRT+ G +++V+VE+ N KY V VE+ L+
Sbjct: 65 AHRRKALSASRISLVPSAKRVPTYLFRTDIGGQVKVLVERT-NGKYKVLVEVLPLELSYA 123
Query: 353 VEEPVMIWGLFISNSTSLMQLDFQQSSVDGQC---ETRFVGG-----AVELEFEADLAPF 508
E VM+WGLF S+++ M LD + DG+ ET FV G VEL+FEA LAPF
Sbjct: 124 HSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVELDFEASLAPF 183
Query: 509 YVXXXXXXXXXXXXXXXTIRSHRNTNFTVPVGFGAGHPSPLGVSFSNNGSVNFAIVSCDA 688
Y+ IRSHRNTNF VPVG +GHP+PLG+SF +GSVNFA+ S A
Sbjct: 184 YISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGSVNFALFSRSA 243
Query: 689 ESVVLCLYADATSEEPSLEIDLDPYINRSGNIWHALLYSPMPFVSYGYRCRSGVENKDNR 868
SVVLCLY D + E+PSLEIDLDPYINRSG+IWHA L +PF +YGYRC++ K
Sbjct: 244 RSVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDCSLPFKTYGYRCKATTSGKGEL 303
Query: 869 VLLDPYAKVIG-----VSGLNQPRKWLGNLCEEPVFDWSGEVRPNLPMEKMIVYRLNVGR 1033
VLLDPYAKVI G K+LG LC EP +DWSG+V P+LPMEK+I+YRLNV +
Sbjct: 304 VLLDPYAKVIRRVIPRQGGSEIRPKYLGELCLEPGYDWSGDVPPSLPMEKLIIYRLNVTQ 363
Query: 1034 FTKDKSSKLADGVAGSFSAISEKLQHFKDLGVNAILLEPVFPFGEQNGPYFPLHFFSPDS 1213
FTKDKSSKL D +AG+FS ISEK HFKDLGVNA+LLEP+FPF EQ GPYFP HFFSP +
Sbjct: 364 FTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNAMLLEPIFPFDEQKGPYFPWHFFSPGN 423
Query: 1214 LYGPSRDAVNVANSMKDMVKKLHA 1285
+YGPS D ++ SMKDMVKKLHA
Sbjct: 424 MYGPSGDPLSAIKSMKDMVKKLHA 447
>ref|XP_004247144.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
[Solanum lycopersicum]
Length = 878
Score = 397 bits (1020), Expect = e-108
Identities = 207/384 (53%), Positives = 261/384 (67%), Gaps = 13/384 (3%)
Frame = +2
Query: 173 SQKLKAVATPDVSVVKTSEKVATYRFRTEFGDRLRVVVEKKYNNKYGVHVELESLQFFGG 352
+ +LKA++ +S+V ++++V TY FRT+ G +++V VEK N KY V VE+ L+
Sbjct: 65 AHRLKALSGSRISLVPSAKRVPTYLFRTDIGCQVKVFVEKT-NGKYKVLVEVLPLELSDA 123
Query: 353 VEEPVMIWGLFISNSTSLMQLDFQQSSVDGQC---ETRFVGG-----AVELEFEADLAPF 508
E VM+WGLF S+++ M LD + DG+ ET FV G VEL+FE L PF
Sbjct: 124 HSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVELDFEVSLVPF 183
Query: 509 YVXXXXXXXXXXXXXXXTIRSHRNTNFTVPVGFGAGHPSPLGVSFSNNGSVNFAIVSCDA 688
Y+ IRSHRNTNF VPVG +GHP+PLG+SF +G VNFA+ S A
Sbjct: 184 YISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGYVNFALYSRSA 243
Query: 689 ESVVLCLYADATSEEPSLEIDLDPYINRSGNIWHALLYSPMPFVSYGYRCRSGVENKDNR 868
+ VVLCLY D + E+PSLEIDLDPYIN+SG+IWHA L +PF +YGYR ++ K
Sbjct: 244 KGVVLCLYDDISVEKPSLEIDLDPYINQSGDIWHAALDCSLPFKTYGYRFKAATSGKGEL 303
Query: 869 VLLDPYAKVIGV-----SGLNQPRKWLGNLCEEPVFDWSGEVRPNLPMEKMIVYRLNVGR 1033
VLLDPYAKVI G K+LG LC EP +DWSG+V P+LPMEK+I+YR+NV
Sbjct: 304 VLLDPYAKVIRSVIPRQGGSEIRPKYLGELCLEPGYDWSGDVPPSLPMEKLIIYRINVTH 363
Query: 1034 FTKDKSSKLADGVAGSFSAISEKLQHFKDLGVNAILLEPVFPFGEQNGPYFPLHFFSPDS 1213
FTKDKSSKL D +AG+FS ISEK H KDLGVNA+LLEP+FPF EQ GPY+P HFFSP +
Sbjct: 364 FTKDKSSKLPDNLAGTFSGISEKWHHLKDLGVNAMLLEPIFPFDEQKGPYYPWHFFSPGN 423
Query: 1214 LYGPSRDAVNVANSMKDMVKKLHA 1285
+YGPS D ++V SMKDMVKKLHA
Sbjct: 424 MYGPSGDPLSVIKSMKDMVKKLHA 447
>ref|XP_007201750.1| hypothetical protein PRUPE_ppa001199mg [Prunus persica]
gi|462397150|gb|EMJ02949.1| hypothetical protein
PRUPE_ppa001199mg [Prunus persica]
Length = 883
Score = 370 bits (949), Expect = e-100
Identities = 196/373 (52%), Positives = 249/373 (66%), Gaps = 20/373 (5%)
Frame = +2
Query: 227 EKVATYRFRTEFGDRLRVVVEKKYNNKYGVHVELESLQFFGGVEEPVMIWGLFISNSTSL 406
+KV+TY FRTE GD + V V K+ N KY V++E+ S V+ WG++ ++S+
Sbjct: 96 DKVSTYLFRTEIGDVVNVFVRKR-NAKYTVNIEVPSSHLSSNDRRLVLRWGMYRADSSCF 154
Query: 407 MQLDFQQSSVDGQCET-----------RFVGGAVELEFEADLAPFYVXXXXXXXXXXXXX 553
+ LDF+ S+ + T RF +ELEFEA PFY
Sbjct: 155 VPLDFKSSTPNDTTTTLETPLIQTSSGRFT---LELEFEAKQIPFYFSFILTSPADANVS 211
Query: 554 XXTIRSHRNTNFTVPVGFGAGHPSPLGVSFSNNGSVNFAIVSCDAESVVLCLYADATSEE 733
IRSHR TNF VPVGFG G+P PLG++FSN+GS+NFAI S +AESV LCLY + T+E+
Sbjct: 212 DMEIRSHRKTNFCVPVGFGRGYPGPLGLTFSNDGSMNFAIFSRNAESVALCLYDNTTAEK 271
Query: 734 PSLEIDLDPYINRSGNIWHALLYSPMPFVSYGYRCRSGV--ENKDN----RVLLDPYAKV 895
P+LE+DLDPY+NRSG+IWH S FVSYGY+ + + NK+N VLLDPYAKV
Sbjct: 272 PALELDLDPYVNRSGDIWHVSFGSAWSFVSYGYKFKGNLLLTNKNNFDEGHVLLDPYAKV 331
Query: 896 IGVSGLNQPR---KWLGNLCEEPVFDWSGEVRPNLPMEKMIVYRLNVGRFTKDKSSKLAD 1066
I S N K+LG LCEEP FDW+G+VRP+L MEK++VYRLNV RFT+ KSS+L
Sbjct: 332 IAKSIPNNHGTGLKYLGRLCEEPAFDWAGDVRPDLSMEKLVVYRLNVTRFTEHKSSQLPT 391
Query: 1067 GVAGSFSAISEKLQHFKDLGVNAILLEPVFPFGEQNGPYFPLHFFSPDSLYGPSRDAVNV 1246
+ GSFS ++EKL+HFKDLGVNA+LLEP+FPF EQ GPYFP HFFSP +GPSR V+
Sbjct: 392 NIGGSFSGLTEKLEHFKDLGVNAVLLEPIFPFDEQKGPYFPHHFFSPMDCFGPSRGPVSA 451
Query: 1247 ANSMKDMVKKLHA 1285
NSMK+MV+K HA
Sbjct: 452 VNSMKEMVRKFHA 464
>ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like [Cucumis sativus]
Length = 885
Score = 368 bits (944), Expect = 4e-99
Identities = 196/377 (51%), Positives = 256/377 (67%), Gaps = 21/377 (5%)
Frame = +2
Query: 218 KTSEKVATYRFRTEFGDRLRVVVEKKYNNKYGVHVELESLQFFGGVEEPVMIWGLFISNS 397
K +VATY FRTEFGD + V V KK + + V++E+ S+Q E ++ WG++ S+S
Sbjct: 93 KEQRRVATYLFRTEFGDLVNVFVGKK-GSTFTVNIEVPSMQLVSIDEALLLSWGVYRSDS 151
Query: 398 TSLMQLDFQQSSVD---GQCETRFVGGA-----VELEFEADLAPFYVXXXXXXXXXXXXX 553
+L+ +F+ S D G ET FV + VELEF+A PFY+
Sbjct: 152 -ALVTPNFESSPPDETTGATETPFVKTSEGKFSVELEFDAKHTPFYLSFVLKYPMGVDSG 210
Query: 554 XXTIRSHRNTNFTVPVGFGAGHPSPLGVSFSNNGSVNFAIVSCDAESVVLCLYADATSEE 733
IRSH+ T+F+VPVGFG G+PSPLG+S S +GSVNF+I S AES+VLCLY D+TSE+
Sbjct: 211 SSEIRSHKKTSFSVPVGFGRGYPSPLGLSISGDGSVNFSIFSSSAESLVLCLYNDSTSEK 270
Query: 734 PSLEIDLDPYINRSGNIWHALLYSPMPFVSYGYRCRSGV--ENKD----NRVLLDPYAKV 895
P LE+DLDPYINRSGNIWHA FVSYGY+C+ EN+D +R+++DPYAK+
Sbjct: 271 PLLELDLDPYINRSGNIWHASFEGASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKI 330
Query: 896 IGVS-------GLNQPRKWLGNLCEEPVFDWSGEVRPNLPMEKMIVYRLNVGRFTKDKSS 1054
+ S GL P K+LG + + P FDW GEV PNLPMEK+ VYRLNV RFT DKSS
Sbjct: 331 LAPSIPKSSGQGLGLPSKFLGQISKVPTFDWDGEVHPNLPMEKLFVYRLNVERFTMDKSS 390
Query: 1055 KLADGVAGSFSAISEKLQHFKDLGVNAILLEPVFPFGEQNGPYFPLHFFSPDSLYGPSRD 1234
+L +AG+FS +++KL HFK+LGVNA+LLEP+F F E+ GPYFP HFFSP + YGPS
Sbjct: 391 QLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGA 450
Query: 1235 AVNVANSMKDMVKKLHA 1285
+++ NSMK+MVK+LHA
Sbjct: 451 SISAINSMKEMVKELHA 467
>ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
[Cucumis sativus]
Length = 885
Score = 367 bits (943), Expect = 5e-99
Identities = 196/377 (51%), Positives = 256/377 (67%), Gaps = 21/377 (5%)
Frame = +2
Query: 218 KTSEKVATYRFRTEFGDRLRVVVEKKYNNKYGVHVELESLQFFGGVEEPVMIWGLFISNS 397
K +VATY FRTEFGD + V V KK + + V++E+ S+Q E ++ WG++ S+S
Sbjct: 93 KEQRRVATYLFRTEFGDLVNVFVGKK-GSTFTVNIEVPSMQLVSIDEALLLSWGVYRSDS 151
Query: 398 TSLMQLDFQQSSVD---GQCETRFVGGA-----VELEFEADLAPFYVXXXXXXXXXXXXX 553
+L+ +F+ S D G ET FV + VELEF+A PFY+
Sbjct: 152 -ALVTPNFESSPPDETTGATETPFVKTSEGKFSVELEFDAKHTPFYLSFVLKYPMGVDSG 210
Query: 554 XXTIRSHRNTNFTVPVGFGAGHPSPLGVSFSNNGSVNFAIVSCDAESVVLCLYADATSEE 733
IRSH+ T+F+VPVGFG G+PSPLG+S S +GSVNF+I S AES+VLCLY D+TSE+
Sbjct: 211 SSEIRSHKKTSFSVPVGFGRGYPSPLGLSISGDGSVNFSIFSSSAESLVLCLYNDSTSEK 270
Query: 734 PSLEIDLDPYINRSGNIWHALLYSPMPFVSYGYRCRSGV--ENKD----NRVLLDPYAKV 895
P LE+DLDPYINRSGNIWHA FVSYGY+C+ EN+D +R+++DPYAK+
Sbjct: 271 PLLELDLDPYINRSGNIWHASFEGASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKI 330
Query: 896 IGVS-------GLNQPRKWLGNLCEEPVFDWSGEVRPNLPMEKMIVYRLNVGRFTKDKSS 1054
+ S GL P K+LG + + P FDW GEV PNLPMEK+ VYRLNV RFT DKSS
Sbjct: 331 LAPSIPKSSGQGLGLPSKFLGQISKVPTFDWDGEVHPNLPMEKLXVYRLNVERFTMDKSS 390
Query: 1055 KLADGVAGSFSAISEKLQHFKDLGVNAILLEPVFPFGEQNGPYFPLHFFSPDSLYGPSRD 1234
+L +AG+FS +++KL HFK+LGVNA+LLEP+F F E+ GPYFP HFFSP + YGPS
Sbjct: 391 QLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGA 450
Query: 1235 AVNVANSMKDMVKKLHA 1285
+++ NSMK+MVK+LHA
Sbjct: 451 SISAINSMKEMVKELHA 467
>ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic-like [Fragaria vesca subsp.
vesca]
Length = 868
Score = 367 bits (942), Expect = 6e-99
Identities = 195/362 (53%), Positives = 243/362 (67%), Gaps = 8/362 (2%)
Frame = +2
Query: 224 SEKVATYRFRTEFGDRLRVVVEKKYNNKYGVHVELESLQFFGGVEEPVMIWGLFISNSTS 403
+ KV+TY FRTE GD + V V N Y V+VEL SLQ + V+ WG++ ++S+S
Sbjct: 91 TNKVSTYLFRTENGDLINVFVRDNTVN-YSVYVELSSLQLSSAGDRLVISWGMYRADSSS 149
Query: 404 LMQLDFQQSSVDGQCETRFVGGAVELEFEADLAPFYVXXXXXXXXXXXXXXXTIRSHRNT 583
L LDF ++ F +ELEFEA PFY+ IRSHR T
Sbjct: 150 LKPLDFIETPFTKTSSGSFT---LELEFEAKQTPFYLSFTVKSLADANLSGLEIRSHRKT 206
Query: 584 NFTVPVGFGAGHPSPLGVSFSNNGSVNFAIVSCDAESVVLCLYADATSEEPSLEIDLDPY 763
NF VPVGFG G P+PLG+S+S++GS+NFAI S +AESVVLCLY D T++EP+LE+DLDPY
Sbjct: 207 NFCVPVGFGRGCPTPLGLSYSSDGSINFAIFSRNAESVVLCLYDDPTAQEPALELDLDPY 266
Query: 764 INRSGNIWHALLYSPMPFVSYGYRCRSGVENKDN-----RVLLDPYAKVIGVSGLNQPR- 925
+NRSG+IWHA S F+SYGYR + N VLLDPYAKVI S N
Sbjct: 267 VNRSGDIWHASFESAWTFLSYGYRFKGTSLRNTNSLDEGNVLLDPYAKVIDESIANNRGT 326
Query: 926 --KWLGNLCEEPVFDWSGEVRPNLPMEKMIVYRLNVGRFTKDKSSKLADGVAGSFSAISE 1099
K LG LCEEPVFDW G+VRP LP+EK++VYRLN+ RFT+ KSSKL VAG+FS +++
Sbjct: 327 GLKLLGRLCEEPVFDWDGDVRPLLPIEKLVVYRLNLKRFTEHKSSKLPTNVAGTFSGLTQ 386
Query: 1100 KLQHFKDLGVNAILLEPVFPFGEQNGPYFPLHFFSPDSLYGPSRDAVNVANSMKDMVKKL 1279
KL HF+DLGVNAILLEP+FPF E+ GPYFP HFFSP + +GPS + NSMK+MVK+
Sbjct: 387 KLDHFQDLGVNAILLEPIFPFDEEKGPYFPCHFFSPMNCFGPSGGPIASINSMKEMVKEF 446
Query: 1280 HA 1285
HA
Sbjct: 447 HA 448
>gb|EXB39337.1| Isoamylase 2 [Morus notabilis]
Length = 825
Score = 363 bits (932), Expect = 9e-98
Identities = 200/409 (48%), Positives = 262/409 (64%), Gaps = 19/409 (4%)
Frame = +2
Query: 116 EPKAECRRFMKRVERISYDSQKLKAVATPDV-------SVVKTSEKVATYRFRTEFGDRL 274
E K C F K +S + V+ V + V+ +EKV+TY FRTE G +
Sbjct: 4 ERKLLCGNFEKMPRNLSVEVNATSRVSIEPVQKRLSRNTKVEEAEKVSTYLFRTEVGGLV 63
Query: 275 RVVVEKKYNNKYGVHVELESLQFFGGVEEPVMIWGLFISNSTSLMQLDFQQSSVDGQCET 454
V V KK N V+VE+ SL+ + WG++ S+S+S + F+ S+ ET
Sbjct: 64 NVFVRKKKVN-CTVYVEVSSLELPRSDGTLGLSWGIYRSDSSSFLPSHFETST---PVET 119
Query: 455 RFVGGA-----VELEFEADLAPFYVXXXXXXXXXXXXXXXTIRSHRNTNFTVPVGFGAGH 619
F + VE EFEA P Y+ IRSHR TNF VP+GF +GH
Sbjct: 120 PFTKNSFGRYTVEFEFEAKEIPCYLSFLLKSPRDNDSSGLDIRSHRKTNFCVPLGFSSGH 179
Query: 620 PSPLGVSFSNNGSVNFAIVSCDAESVVLCLYADATSEEPSLEIDLDPYINRSGNIWHALL 799
P+PLG+SFS +GS+NF+I S AESVVLCLY D T++ P+LE+DLDPY+NR+G+IWHA
Sbjct: 180 PTPLGLSFSRDGSLNFSIFSRSAESVVLCLYDDTTADNPALELDLDPYVNRTGDIWHASF 239
Query: 800 YSPMPFVSYGYRCRS----GVENKDNRVLLDPYAKVIGVSGLNQPR---KWLGNLCEEPV 958
S FVSYGYR + G +++D R++LDPYAK+IG S + K+LG + +EP
Sbjct: 240 ESSSTFVSYGYRLKGSRLKGKKDEDARIVLDPYAKIIGKSTSSDHGIGPKYLGRISKEPG 299
Query: 959 FDWSGEVRPNLPMEKMIVYRLNVGRFTKDKSSKLADGVAGSFSAISEKLQHFKDLGVNAI 1138
FDWSG++RPNLP+EK+ VYRLNV FT+ KSS+L+ +AG+FS + EKL+HF DLGVNA+
Sbjct: 300 FDWSGDIRPNLPIEKLTVYRLNVMHFTEHKSSQLSPDIAGTFSGLIEKLEHFTDLGVNAV 359
Query: 1139 LLEPVFPFGEQNGPYFPLHFFSPDSLYGPSRDAVNVANSMKDMVKKLHA 1285
LLEP+FPF EQ GP FP HFFSP SLYGPS AV+ N+MK+MVKKLHA
Sbjct: 360 LLEPIFPFDEQKGPCFPCHFFSPTSLYGPSGGAVSAINTMKEMVKKLHA 408
>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
isoamylase, putative [Ricinus communis]
Length = 872
Score = 361 bits (926), Expect = 4e-97
Identities = 179/368 (48%), Positives = 246/368 (66%), Gaps = 16/368 (4%)
Frame = +2
Query: 230 KVATYRFRTEFGDRLRVVVEKKYNNKYGVHVELESLQFFGGVEEPVMIWGLFISNSTSLM 409
KV+TY FRT+FG ++V+V KK N KY V++E+ SL+ ++IWG++ S+S+ M
Sbjct: 88 KVSTYLFRTQFGGHVKVLVRKK-NAKYAVYIEVSSLELGTTDYRLMLIWGIYRSDSSCFM 146
Query: 410 QLDFQQSSVDGQ------CETRFVGGAVELEFEADLAPFYVXXXXXXXXXXXXXXXTIRS 571
LD Q + + + + F A+ELEFE PFY+ I++
Sbjct: 147 PLDSQNFAPNARKMDTALVQNSFGTFALELEFEPKQTPFYLSFLLKSKLNTDASGLEIKN 206
Query: 572 HRNTNFTVPVGFGAGHPSPLGVSFSNNGSVNFAIVSCDAESVVLCLYADATSEEPSLEID 751
H+N NF VP+GF +G PSPLG+SFS +GS+NFA S + E +VLCLY D+T+++P+LE+D
Sbjct: 207 HKNANFCVPIGFNSGDPSPLGLSFSTDGSMNFAFFSRNVEGLVLCLYDDSTTDKPALELD 266
Query: 752 LDPYINRSGNIWHALLYSPMPFVSYGYRCRSGVENKDNR------VLLDPYAKVIGVS-- 907
LDPY+NR+G++WHA L F SYGYRC+ + + VLLDPYA+VI S
Sbjct: 267 LDPYVNRTGDVWHASLEGAWTFTSYGYRCKGAILQGNTSKVDMECVLLDPYARVIASSMT 326
Query: 908 --GLNQPRKWLGNLCEEPVFDWSGEVRPNLPMEKMIVYRLNVGRFTKDKSSKLADGVAGS 1081
G K+LG LCEEP F+W ++RPNL MEK+IVYRLNV RFT+ KS KL +AG+
Sbjct: 327 DHGSRLSAKYLGRLCEEPAFEWGSDIRPNLAMEKLIVYRLNVKRFTEHKSGKLYSDIAGT 386
Query: 1082 FSAISEKLQHFKDLGVNAILLEPVFPFGEQNGPYFPLHFFSPDSLYGPSRDAVNVANSMK 1261
F+ + EK+ HF++LGVNA+LLEP+FPF EQ GPYFP HFFSP ++YGPS +++ SMK
Sbjct: 387 FAGLIEKMDHFRNLGVNAVLLEPIFPFDEQKGPYFPYHFFSPSNIYGPSGGSISAITSMK 446
Query: 1262 DMVKKLHA 1285
+MVK+LHA
Sbjct: 447 EMVKELHA 454
>ref|XP_006479763.1| PREDICTED: isoamylase 2, chloroplastic-like isoform X1 [Citrus
sinensis] gi|568852193|ref|XP_006479764.1| PREDICTED:
isoamylase 2, chloroplastic-like isoform X2 [Citrus
sinensis] gi|568852195|ref|XP_006479765.1| PREDICTED:
isoamylase 2, chloroplastic-like isoform X3 [Citrus
sinensis]
Length = 889
Score = 355 bits (911), Expect = 2e-95
Identities = 199/395 (50%), Positives = 254/395 (64%), Gaps = 19/395 (4%)
Frame = +2
Query: 158 RISYDSQKLKAVATPDVSVVKTSEKVATYRFRTEFGDRLRVVVEKKYNNKYGVHVELESL 337
R+S D + + V TP V + +KV+TY FRT+ G + V V +K N Y V +++ SL
Sbjct: 80 RVSVD--ETQPVFTPTTEVDEL-QKVSTYLFRTDLGGLVSVSVGEK-NVNYAVTIDVSSL 135
Query: 338 QFFGGVEEPVMIWGLFISNSTSLMQLDFQQSSVD---GQCETRFVGG-----AVELEFEA 493
Q G + V+IWG++ S+S+S M L Q + D G ET F A+EL FEA
Sbjct: 136 QTTRGGHKLVLIWGVYTSDSSSYMHLGSQCFTPDAKTGSIETPFTPTSFDTFALELGFEA 195
Query: 494 DLAPFYVXXXXXXXXXXXXXXXTIRSHRNTNFTVPVGFGAGHPSPLGVSFSNNGSVNFAI 673
PFY+ IR+H + F VPVG AG PSP+G+SFS +GS+NFAI
Sbjct: 196 KQTPFYLSFLLKSPLRDGSGDFEIRNHLSAKFCVPVGLNAGVPSPMGLSFSIDGSLNFAI 255
Query: 674 VSCDAESVVLCLYADATSEEPSLEIDLDPYINRSGNIWHALLYSPMPFVSYGYRCRSGVE 853
S +A+ VVLCLY D T++ P+LE+DLDPYINRSG+IWHA + S FVSYGYR +
Sbjct: 256 FSRNAQGVVLCLYDDTTADRPALELDLDPYINRSGDIWHASMESTWNFVSYGYRFKGSFS 315
Query: 854 NKDN------RVLLDPYAKVIGVS-----GLNQPRKWLGNLCEEPVFDWSGEVRPNLPME 1000
D VLLDPYAK+I S L P K+LG LC+EP FDW G+V NLPME
Sbjct: 316 QGDGYKSHLESVLLDPYAKIIVNSIPNHHDLGLPPKYLGRLCKEPDFDWGGDVHLNLPME 375
Query: 1001 KMIVYRLNVGRFTKDKSSKLADGVAGSFSAISEKLQHFKDLGVNAILLEPVFPFGEQNGP 1180
K++VYRLNV RF++ KSSKL +AG+FS ++EK+ H KDLGVNAILLEP+ F EQ GP
Sbjct: 376 KLVVYRLNVMRFSEHKSSKLPPDIAGTFSGVTEKVHHLKDLGVNAILLEPILSFDEQKGP 435
Query: 1181 YFPLHFFSPDSLYGPSRDAVNVANSMKDMVKKLHA 1285
YFP HFFSP L+GPSR +++ NSMK+MVKKLHA
Sbjct: 436 YFPRHFFSPTKLHGPSRGSISAINSMKEMVKKLHA 470
>ref|XP_006444132.1| hypothetical protein CICLE_v10023436mg [Citrus clementina]
gi|557546394|gb|ESR57372.1| hypothetical protein
CICLE_v10023436mg [Citrus clementina]
Length = 840
Score = 353 bits (907), Expect = 7e-95
Identities = 196/384 (51%), Positives = 248/384 (64%), Gaps = 19/384 (4%)
Frame = +2
Query: 191 VATPDVSVVKTSEKVATYRFRTEFGDRLRVVVEKKYNNKYGVHVELESLQFFGGVEEPVM 370
V TP V + +KV+TY FRT+ G + V V +K N Y V +++ SLQ G + V+
Sbjct: 40 VFTPTTEVDEL-QKVSTYLFRTDLGGLVSVSVGEK-NVNYAVTIDVSSLQTTRGGHKLVL 97
Query: 371 IWGLFISNSTSLMQLDFQQSSVD---GQCETRFVGG-----AVELEFEADLAPFYVXXXX 526
IWG++ S+S+S M L Q + D G ET F A+EL FEA PFY+
Sbjct: 98 IWGVYTSDSSSYMHLGSQCFTPDAKTGSIETPFTPTSFDTFALELGFEAKQTPFYLSFLL 157
Query: 527 XXXXXXXXXXXTIRSHRNTNFTVPVGFGAGHPSPLGVSFSNNGSVNFAIVSCDAESVVLC 706
IR+H + F VPVG AG PSP+G+SFS +GS+NFAI S +A+ VVLC
Sbjct: 158 KSPLRDGSGDFEIRNHLSAKFCVPVGLNAGVPSPMGLSFSIDGSLNFAIFSRNAQGVVLC 217
Query: 707 LYADATSEEPSLEIDLDPYINRSGNIWHALLYSPMPFVSYGYRCRSGVENKDN------R 868
LY D T++ P+LE+DLDPYINRSG+IWHA + S FVSYGYR + D
Sbjct: 218 LYDDTTADRPALELDLDPYINRSGDIWHASMESTWNFVSYGYRFKGSFSQGDGYKSHLES 277
Query: 869 VLLDPYAKVIGVS-----GLNQPRKWLGNLCEEPVFDWSGEVRPNLPMEKMIVYRLNVGR 1033
VLLDPYAK+I S L P K+LG LC+EP FDW G+V NLPMEK++VYRLNV R
Sbjct: 278 VLLDPYAKIIVNSIPNHHDLGLPPKYLGRLCKEPDFDWGGDVHLNLPMEKLVVYRLNVMR 337
Query: 1034 FTKDKSSKLADGVAGSFSAISEKLQHFKDLGVNAILLEPVFPFGEQNGPYFPLHFFSPDS 1213
F++ KSSKL +AG+FS ++EK+ H KDLGVNAILLEP+ F EQ GPYFP HFFSP
Sbjct: 338 FSEHKSSKLPPDIAGTFSGVTEKVHHLKDLGVNAILLEPILSFDEQKGPYFPRHFFSPTK 397
Query: 1214 LYGPSRDAVNVANSMKDMVKKLHA 1285
L+GPSR +++ NSMK+MVKKLHA
Sbjct: 398 LHGPSRGSISAINSMKEMVKKLHA 421
>ref|XP_007050677.1| Debranching enzyme 1 [Theobroma cacao] gi|508702938|gb|EOX94834.1|
Debranching enzyme 1 [Theobroma cacao]
Length = 867
Score = 352 bits (903), Expect = 2e-94
Identities = 194/402 (48%), Positives = 256/402 (63%), Gaps = 22/402 (5%)
Frame = +2
Query: 146 KRVERISYDSQKLKAVATPDVSVVKTSE-----------KVATYRFRTEFGDRLRVVVEK 292
+ V + Y + L+ A VSV +T + K++TY FRTE G +++V V K
Sbjct: 57 QNVMQSRYWNLDLRCFAASRVSVEQTEQIFTSTQVDELKKLSTYLFRTEIGGQVKVFVRK 116
Query: 293 KYNNKYGVHVELESLQFFGGVEEPVMIWGLFISNSTSLMQLDFQQSSVDGQCETRFVGG- 469
+ N + V +E+ SLQ G + V+ G+ S+ D + + ET F+
Sbjct: 117 RSVN-HVVDIEVSSLQLSGDESQLVLSGGIHRSDH------DIKNRII----ETPFIAKS 165
Query: 470 ----AVELEFEADLAPFYVXXXXXXXXXXXXXXXTIRSHRNTNFTVPVGFGAGHPSPLGV 637
A+ELEFEA APFY IR+HR TNF VPVGF G+P+PLG+
Sbjct: 166 SSELALELEFEAKEAPFYFSFLLKAPSGANLSGSEIRTHRKTNFCVPVGFNQGYPAPLGL 225
Query: 638 SFSNNGSVNFAIVSCDAESVVLCLYADATSEEPSLEIDLDPYINRSGNIWHALLYSPMPF 817
SFS +GS+NFA+ S +AES+VLCLY D SE+P+LE+DLDP++NR+G+IWHA + F
Sbjct: 226 SFSTDGSMNFAVYSRNAESLVLCLYDDTASEKPALELDLDPFVNRTGDIWHASIEGSWTF 285
Query: 818 VSYGYRCRSGVENKD-NRVLLDPYAKVIGVSGLNQPR-----KWLGNLCEEPVFDWSGEV 979
V YGYRC+ + + RVLLDPYA++IG S N K LG LC+EP FDWS +V
Sbjct: 286 VRYGYRCKGDTDAFNAERVLLDPYARIIGSSIPNHHESGLLLKHLGRLCKEPAFDWSSDV 345
Query: 980 RPNLPMEKMIVYRLNVGRFTKDKSSKLADGVAGSFSAISEKLQHFKDLGVNAILLEPVFP 1159
PNLPMEK++VYRLNV FT+DKSSKL V G+F+ ++EK+QH KDLGVNA+LLEP+F
Sbjct: 346 CPNLPMEKLVVYRLNVLHFTEDKSSKLPADVVGTFAGVTEKVQHLKDLGVNAVLLEPIFT 405
Query: 1160 FGEQNGPYFPLHFFSPDSLYGPSRDAVNVANSMKDMVKKLHA 1285
F EQ GPYFP HFFSP +LYGPS +V+ NS+K+MVK LHA
Sbjct: 406 FDEQKGPYFPCHFFSPTNLYGPSNGSVSAINSIKEMVKNLHA 447
>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
Length = 882
Score = 352 bits (903), Expect = 2e-94
Identities = 192/397 (48%), Positives = 257/397 (64%), Gaps = 30/397 (7%)
Frame = +2
Query: 185 KAVATPDVSVVKTSEKVAT-----------YRFRTEFGDRLRVVVEKKYNNKYGVHVELE 331
K AT +++ +T + +T Y F TE G +++V+V KK N KY V +E+
Sbjct: 70 KVSATSHIAIEETENRYSTTESEELESRLNYLFLTEIGGQVKVIVGKK-NKKYIVSIEVS 128
Query: 332 SLQFFGGVEEPVMIWGLFISNSTSLMQLDFQQSSVDGQCETRFV--------GGAVELEF 487
SLQ + + ++ WG+F SNS+ M +DFQ + T + A++L+F
Sbjct: 129 SLQLYNSDNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTEIPFMERSSGSFALKLDF 188
Query: 488 EADLAPFYVXXXXXXXXXXXXXXXTIRSHRNTNFTVPVGFGAGHPSPLGVSFSNNGSVNF 667
EA+ APFY+ IRSHR TNF +PVGFG G+P+PLG+SFS++GS NF
Sbjct: 189 EANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFGRGYPAPLGLSFSSDGSPNF 248
Query: 668 AIVSCDAESVVLCLYADATSEEPSLEIDLDPYINRSGNIWHALLYSPMPFVSYGYRCR-- 841
A S +A VVLCLY D TS++P+LEIDLDPY+NR+G+IWHA + S FVSYGYRC+
Sbjct: 249 AFFSRNAGGVVLCLY-DGTSDKPALEIDLDPYVNRTGDIWHASMESVGSFVSYGYRCKEA 307
Query: 842 ----SGVENKDNRVLLDPYAKVIGVS-----GLNQPRKWLGNLCEEPVFDWSGEVRPNLP 994
SG V LDPYAK+I S GL +P+ LG L +EP F+W+ +V P +P
Sbjct: 308 NLQDSGETLHTEHVHLDPYAKLIRNSFSDDHGL-KPQPRLGELQKEPAFNWNDDVHPYIP 366
Query: 995 MEKMIVYRLNVGRFTKDKSSKLADGVAGSFSAISEKLQHFKDLGVNAILLEPVFPFGEQN 1174
MEK++VYRLNV FTKD+SS++A +AG+FS + EKL HFKDLGVNA+LLEP+F F EQ
Sbjct: 367 MEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKDLGVNAVLLEPIFSFDEQK 426
Query: 1175 GPYFPLHFFSPDSLYGPSRDAVNVANSMKDMVKKLHA 1285
GPYFP HFFSP ++YGPS V+ NS+K+MVK+LHA
Sbjct: 427 GPYFPFHFFSPMNVYGPSSGPVSTINSVKEMVKRLHA 463
>ref|XP_006386822.1| hypothetical protein POPTR_0002s22530g [Populus trichocarpa]
gi|550345605|gb|ERP64619.1| hypothetical protein
POPTR_0002s22530g [Populus trichocarpa]
Length = 857
Score = 349 bits (896), Expect = 1e-93
Identities = 188/378 (49%), Positives = 248/378 (65%), Gaps = 20/378 (5%)
Frame = +2
Query: 212 VVKTSEKVATYRFRTEFGDRLRVVVEKKYNNKYGVHVELESLQFFGGVEEPVM-IWGLFI 388
+V ++V+TYRFRTE G +++ V K N KY V+VE+ SL+ +M IWG++
Sbjct: 76 LVDQLKEVSTYRFRTEIGGHVKISVGK-INGKYAVYVEVSSLELGASDNISLMLIWGIYT 134
Query: 389 SNSTSLMQLDFQQSS-------VDGQCETRFVGGAVELEFEADLAPFYVXXXXXXXXXXX 547
S+S+ M LD + + C RF A ELEFEA PFY+
Sbjct: 135 SDSSCFMPLDSSSHARTRETPLLQNSC-ARF---ATELEFEAKQTPFYLSFFLKPTSSVV 190
Query: 548 XXXXTIRSHRNTNFTVPVGFGAGHPSPLGVSFSNNGSVNFAIVSCDAESVVLCLYADATS 727
IR+H +NF VP+GF +G+P+PLG+SFS +GS+NFA S +A VLCLY D+TS
Sbjct: 191 E----IRNHNKSNFCVPIGFDSGYPTPLGLSFSTDGSMNFAFFSRNAAGCVLCLYDDSTS 246
Query: 728 EEPSLEIDLDPYINRSGNIWHALLYSPMPFVSYGYRCRSGVENKD------NRVLLDPYA 889
+P+LE+DLDPY+NRSG+IWHA L F+SYGYRC+ D RVLLDPY+
Sbjct: 247 GKPALELDLDPYVNRSGDIWHASLEGAWTFLSYGYRCKGAALQSDADKFDAGRVLLDPYS 306
Query: 890 KVI------GVSGLNQPRKWLGNLCEEPVFDWSGEVRPNLPMEKMIVYRLNVGRFTKDKS 1051
K+I VSGL K+LG LC+EPVFDWS +V P+L MEK++VYR+NV RFTKD S
Sbjct: 307 KIIINSVTDNVSGLLP--KYLGRLCKEPVFDWSDDVPPHLEMEKLVVYRVNVMRFTKDNS 364
Query: 1052 SKLADGVAGSFSAISEKLQHFKDLGVNAILLEPVFPFGEQNGPYFPLHFFSPDSLYGPSR 1231
S+++ AG+F+ + EKL HFK+LGVNA+LLE +FPF EQ GPYFP HFFSP ++YGPS
Sbjct: 365 SQISSDAAGTFAGLIEKLSHFKNLGVNAVLLESIFPFDEQKGPYFPCHFFSPSNVYGPSN 424
Query: 1232 DAVNVANSMKDMVKKLHA 1285
+V +SMK+MVK+LHA
Sbjct: 425 GSVAAISSMKEMVKRLHA 442
>gb|AAY89379.1| isoamylase isoform 2 [Nicotiana langsdorffii x Nicotiana sanderae]
Length = 556
Score = 345 bits (886), Expect = 2e-92
Identities = 175/306 (57%), Positives = 215/306 (70%), Gaps = 13/306 (4%)
Frame = +2
Query: 407 MQLDFQQSSVDGQC---ETRFVGG-----AVELEFEADLAPFYVXXXXXXXXXXXXXXXT 562
M LD ++ DG+ ET FV G VEL+FEA LAPFY+
Sbjct: 6 MPLDLKRRGADGKSSAIETPFVQGPSDKVTVELDFEASLAPFYISFYMKSQLVADVKSSE 65
Query: 563 IRSHRNTNFTVPVGFGAGHPSPLGVSFSNNGSVNFAIVSCDAESVVLCLYADATSEEPSL 742
IRSHRNT+F VPVG GHP+PLG+S+ +G++NFA+ S A+SVVLCLY D + E PSL
Sbjct: 66 IRSHRNTSFVVPVGLTTGHPAPLGLSYQPDGTMNFALFSRSAKSVVLCLYDDISVENPSL 125
Query: 743 EIDLDPYINRSGNIWHALLYSPMPFVSYGYRCRSGVENKDNRVLLDPYAKVIGV-----S 907
EIDLDPY+NRSG+IWHA L +PF +YGYRC+ K+ VLLDPY+KVI
Sbjct: 126 EIDLDPYVNRSGDIWHAALDCSLPFKTYGYRCKVATSGKEELVLLDPYSKVIRSVIPRQG 185
Query: 908 GLNQPRKWLGNLCEEPVFDWSGEVRPNLPMEKMIVYRLNVGRFTKDKSSKLADGVAGSFS 1087
G K+LG LC+EP +DWSG+V P+LPMEK+I+YRLNV FTKD SSKL D +AG+FS
Sbjct: 186 GSELRPKYLGELCKEPGYDWSGDVPPSLPMEKLIIYRLNVTEFTKDGSSKLPDDLAGTFS 245
Query: 1088 AISEKLQHFKDLGVNAILLEPVFPFGEQNGPYFPLHFFSPDSLYGPSRDAVNVANSMKDM 1267
I+EKL HFK LGVNA+LLEP+FPF EQ GPYFP HFFSP +++G S D ++V NSM+DM
Sbjct: 246 GITEKLSHFKHLGVNAVLLEPIFPFEEQKGPYFPSHFFSPGNMHGRSSDPLSVINSMEDM 305
Query: 1268 VKKLHA 1285
VKKLHA
Sbjct: 306 VKKLHA 311
>ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
Length = 865
Score = 328 bits (842), Expect = 2e-87
Identities = 194/440 (44%), Positives = 266/440 (60%), Gaps = 38/440 (8%)
Frame = +2
Query: 80 HHKFRKSNVLVFEPKAECRR--------FMKRVERISYDSQKLKAVATPDVSVVKTSEKV 235
HHK ++S F K +R V S + K AT +S+ +T +++
Sbjct: 17 HHKLKRS----FSHKPFIQRKQLWHQLGLQSLVSPFSLNPTSSKLCATSRLSIEETEQQI 72
Query: 236 AT------------YRFRTEFGDRLRVVVEKKYNNKYGVHVELESLQFFGGVEEPVMI-W 376
T Y FRTE G L V + N++Y V++E+ SL GGV E +++ W
Sbjct: 73 GTLTRPEDLKGSLAYLFRTETGGGLVKVHVTRRNDRYSVYIEISSLDISGGVGEALLLCW 132
Query: 377 GLFISNSTSLMQLD---FQQSSVDGQCETRFVGG-----AVELEFEADLAPFYVXXXXXX 532
G++ S+S+ + LD +++ G + V A+ELEF+A P Y+
Sbjct: 133 GVYRSDSSCFVDLDTIGLSENAAMGMNVSPLVQNSDGKFAIELEFDAKHVPLYLSFFLMS 192
Query: 533 XXXXXXXXXTIRSHRNTNFTVPVGFGAGHPSPLGVSFSNNGSVNFAIVSCDAESVVLCLY 712
IRSHR TNF +PVG G+P PLGVS+S +GSVNF+I S AESVVLCLY
Sbjct: 193 SLDSGLE---IRSHRRTNFCMPVGSLPGYPCPLGVSYSPDGSVNFSIFSRHAESVVLCLY 249
Query: 713 ADATSEEPSLEIDLDPYINRSGNIWHALLYSPMPFVSYGYRCRSGV--ENKDN----RVL 874
+ E+P+LE+DLDPY+NR+G+IWH S FVSYGYRCR GV +NKD+ V+
Sbjct: 250 DENGVEKPALELDLDPYVNRTGDIWHVSFESAKGFVSYGYRCRRGVLKKNKDDGFAEHVV 309
Query: 875 LDPYAKVIGVS---GLNQPRKWLGNLCEEPVFDWSGEVRPNLPMEKMIVYRLNVGRFTKD 1045
LDPYAK++G S G+ + LG L +EP FDW G+ P+L MEK++VYRLNV RFT+
Sbjct: 310 LDPYAKIVGNSYPDGVGLVKN-LGCLRKEPFFDWGGDRHPDLSMEKLVVYRLNVKRFTQH 368
Query: 1046 KSSKLADGVAGSFSAISEKLQHFKDLGVNAILLEPVFPFGEQNGPYFPLHFFSPDSLYGP 1225
+SS+L G+AG+F+ +++K+QHFKDLGVNA+LLEPVF F E+ GPYFP HFFS +YGP
Sbjct: 369 ESSQLPSGLAGTFTGLAKKVQHFKDLGVNAVLLEPVFTFDEKKGPYFPCHFFSLMHIYGP 428
Query: 1226 SRDAVNVANSMKDMVKKLHA 1285
S V+ +MK+MVK +HA
Sbjct: 429 SGGPVSAIAAMKEMVKTMHA 448
>ref|XP_002889452.1| ATISA2/BE2/DBE1/ISA2 [Arabidopsis lyrata subsp. lyrata]
gi|297335294|gb|EFH65711.1| ATISA2/BE2/DBE1/ISA2
[Arabidopsis lyrata subsp. lyrata]
Length = 872
Score = 328 bits (840), Expect = 4e-87
Identities = 176/390 (45%), Positives = 249/390 (63%), Gaps = 16/390 (4%)
Frame = +2
Query: 164 SYDSQKLKAVATPDVSVVKTSEKVATYRFRTEFGDRLRVVVEKKYNNKYGVHVELESLQF 343
S S +L+ + + V +KV +Y FRT+ G ++V VE+K KY + V + SL+
Sbjct: 61 SKTSIELREALSTKRAQVDDLKKVTSYSFRTKSGALVKVKVEQK-REKYSIMVYVSSLEL 119
Query: 344 FGGVEEP-VMIWGLFISNSTSLMQLDFQQSSVDGQC---ETRFVGGAVE-----LEFEAD 496
G + VM+WG++ S+S+ + LDF+ SS D Q ET FV ++ LEF+
Sbjct: 120 TGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETPFVKSSLSELMLGLEFDGK 179
Query: 497 LAPFYVXXXXXXXXXXXXXXXTIRSHRNTNFTVPVGFGAGHPSPLGVSFS-NNGSVNFAI 673
+PFY+ + +HR+T+F +PVGF AGHP PLG+S ++ S NFA
Sbjct: 180 ESPFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFAF 239
Query: 674 VSCDAESVVLCLYADATSEEPSLEIDLDPYINRSGNIWHALLYSPMPFVSYGYRCRSGVE 853
S ++ +VVLCLY D T+ +P+LE+DLDPY+NR+G++WHA + + FV YGYRC+
Sbjct: 240 FSRNSTNVVLCLYDDTTTNKPALELDLDPYVNRTGDVWHASIDNTWDFVRYGYRCKETAH 299
Query: 854 NKDN------RVLLDPYAKVIGVSGLNQPRKWLGNLCEEPVFDWSGEVRPNLPMEKMIVY 1015
++++ ++LDPYA VIG S +K+LG+L + P FDW +V PN+P+EK+IVY
Sbjct: 300 SEEDVDVEAEPIVLDPYATVIGKS---VSQKFLGSLFKNPSFDWGEDVSPNIPLEKLIVY 356
Query: 1016 RLNVGRFTKDKSSKLADGVAGSFSAISEKLQHFKDLGVNAILLEPVFPFGEQNGPYFPLH 1195
RLNV FT+ KSSKL VAG+FS ++EK+ H K LGVN +LLEP+F F EQ GPYFP H
Sbjct: 357 RLNVKGFTQHKSSKLPSNVAGTFSGVAEKVSHLKTLGVNGVLLEPIFSFSEQKGPYFPFH 416
Query: 1196 FFSPDSLYGPSRDAVNVANSMKDMVKKLHA 1285
FFSP +YGPS + NSMK+MVKKLH+
Sbjct: 417 FFSPMDIYGPSNSLESAVNSMKEMVKKLHS 446
>gb|AAM98123.1| putative isoamylase [Arabidopsis thaliana]
Length = 882
Score = 326 bits (835), Expect = 2e-86
Identities = 170/369 (46%), Positives = 241/369 (65%), Gaps = 16/369 (4%)
Frame = +2
Query: 227 EKVATYRFRTEFGDRLRVVVEKKYNNKYGVHVELESLQFFGGVEEP-VMIWGLFISNSTS 403
+KV +Y FRT+ G ++V VEKK KY + V + SL+ G + VM+WG++ S+S+
Sbjct: 93 KKVTSYSFRTKAGALVKVKVEKK-REKYSILVYVSSLELSGDDKSRLVMVWGVYRSDSSC 151
Query: 404 LMQLDFQQSSVDGQC---ETRFVGGAVE-----LEFEADLAPFYVXXXXXXXXXXXXXXX 559
+ LDF+ SS D Q ET FV ++ LEF+ +PFY+
Sbjct: 152 FLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGR 211
Query: 560 TIRSHRNTNFTVPVGFGAGHPSPLGVSFS-NNGSVNFAIVSCDAESVVLCLYADATSEEP 736
+ +HR+T+F +PVGF AGHP PLG+S ++ S NF+ S + +VVLCLY D+T+++P
Sbjct: 212 EMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKP 271
Query: 737 SLEIDLDPYINRSGNIWHALLYSPMPFVSYGYRCRSGVENKDN------RVLLDPYAKVI 898
+LE+DLDPY+NR+G++WHA + + FV YGYRC+ +K++ ++LDPYA V+
Sbjct: 272 ALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVV 331
Query: 899 GVSGLNQPRKWLGNLCEEPVFDWSGEVRPNLPMEKMIVYRLNVGRFTKDKSSKLADGVAG 1078
G S +K+LG+L + P FDW +V PN+P+EK++VYRLNV FT+ +SSKL VAG
Sbjct: 332 GKS---VSQKYLGSLSKSPSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAG 388
Query: 1079 SFSAISEKLQHFKDLGVNAILLEPVFPFGEQNGPYFPLHFFSPDSLYGPSRDAVNVANSM 1258
+FS ++EK+ H K LG NA+LLEP+F F EQ GPYFP HFFSP +YGPS + NSM
Sbjct: 389 TFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSM 448
Query: 1259 KDMVKKLHA 1285
K MVKKLH+
Sbjct: 449 KVMVKKLHS 457
>ref|NP_171830.1| Isoamylase 2 [Arabidopsis thaliana] gi|42571321|ref|NP_973751.1|
Isoamylase 2 [Arabidopsis thaliana]
gi|251764780|sp|Q8L735.2|ISOA2_ARATH RecName:
Full=Isoamylase 2, chloroplastic; Short=AtISA2; AltName:
Full=Protein DEBRANCHING ENZYME 1; Flags: Precursor
gi|3850573|gb|AAC72113.1| Similar to gi|1652733 glycogen
operon protein GlgX from Synechocystis sp. genome
gb|D90908. ESTs gb|H36690, gb|AA712462, gb|AA651230 and
gb|N95932 come from this gene [Arabidopsis thaliana]
gi|332189436|gb|AEE27557.1| Isoamylase 2 [Arabidopsis
thaliana] gi|332189437|gb|AEE27558.1| Isoamylase 2
[Arabidopsis thaliana]
Length = 882
Score = 326 bits (835), Expect = 2e-86
Identities = 170/369 (46%), Positives = 241/369 (65%), Gaps = 16/369 (4%)
Frame = +2
Query: 227 EKVATYRFRTEFGDRLRVVVEKKYNNKYGVHVELESLQFFGGVEEP-VMIWGLFISNSTS 403
+KV +Y FRT+ G ++V VEKK KY + V + SL+ G + VM+WG++ S+S+
Sbjct: 93 KKVTSYSFRTKAGALVKVKVEKK-REKYSILVYVSSLELSGDDKSRLVMVWGVYRSDSSC 151
Query: 404 LMQLDFQQSSVDGQC---ETRFVGGAVE-----LEFEADLAPFYVXXXXXXXXXXXXXXX 559
+ LDF+ SS D Q ET FV ++ LEF+ +PFY+
Sbjct: 152 FLPLDFENSSQDSQTHTTETTFVKSSLSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGQ 211
Query: 560 TIRSHRNTNFTVPVGFGAGHPSPLGVSFS-NNGSVNFAIVSCDAESVVLCLYADATSEEP 736
+ +HR+T+F +PVGF AGHP PLG+S ++ S NF+ S + +VVLCLY D+T+++P
Sbjct: 212 EMLTHRDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKP 271
Query: 737 SLEIDLDPYINRSGNIWHALLYSPMPFVSYGYRCRSGVENKDN------RVLLDPYAKVI 898
+LE+DLDPY+NR+G++WHA + + FV YGYRC+ +K++ ++LDPYA V+
Sbjct: 272 ALELDLDPYVNRTGDVWHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVV 331
Query: 899 GVSGLNQPRKWLGNLCEEPVFDWSGEVRPNLPMEKMIVYRLNVGRFTKDKSSKLADGVAG 1078
G S +K+LG+L + P FDW +V PN+P+EK++VYRLNV FT+ +SSKL VAG
Sbjct: 332 GKS---VSQKYLGSLSKSPSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAG 388
Query: 1079 SFSAISEKLQHFKDLGVNAILLEPVFPFGEQNGPYFPLHFFSPDSLYGPSRDAVNVANSM 1258
+FS ++EK+ H K LG NA+LLEP+F F EQ GPYFP HFFSP +YGPS + NSM
Sbjct: 389 TFSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSM 448
Query: 1259 KDMVKKLHA 1285
K MVKKLH+
Sbjct: 449 KVMVKKLHS 457