BLASTX nr result
ID: Mentha22_contig00026748
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Mentha22_contig00026748
(308 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002514582.1| inositol or phosphatidylinositol kinase, put... 127 1e-27
gb|EYU22584.1| hypothetical protein MIMGU_mgv1a002776mg [Mimulus... 127 2e-27
ref|XP_006348640.1| PREDICTED: phosphatidylinositol 4-kinase gam... 127 2e-27
ref|XP_007045737.1| Phosphoinositide 4-kinase gamma 7, gamma 7,u... 126 3e-27
ref|XP_004239016.1| PREDICTED: probable phosphatidylinositol 4-k... 126 3e-27
ref|XP_006363554.1| PREDICTED: phosphatidylinositol 4-kinase gam... 126 4e-27
ref|XP_004237064.1| PREDICTED: probable phosphatidylinositol 4-k... 124 2e-26
ref|XP_004146767.1| PREDICTED: probable phosphatidylinositol 4-k... 124 2e-26
ref|XP_006484264.1| PREDICTED: phosphatidylinositol 4-kinase gam... 123 2e-26
ref|XP_006437843.1| hypothetical protein CICLE_v10030931mg [Citr... 123 2e-26
ref|XP_004135137.1| PREDICTED: probable phosphatidylinositol 4-k... 122 5e-26
gb|EXB44906.1| putative phosphatidylinositol 4-kinase type 2-bet... 119 6e-25
gb|ADK78996.1| phosphatidylinositol 4-kinase [Gossypium barbadense] 118 7e-25
ref|XP_004297194.1| PREDICTED: probable phosphatidylinositol 4-k... 116 4e-24
ref|XP_002316001.1| phosphatidylinositol 3- and 4-kinase family ... 116 4e-24
ref|XP_002267025.1| PREDICTED: probable phosphatidylinositol 4-k... 116 4e-24
emb|CBI27403.3| unnamed protein product [Vitis vinifera] 114 2e-23
ref|XP_002311415.2| phosphatidylinositol 3- and 4-kinase family ... 112 7e-23
gb|EPS72797.1| hypothetical protein M569_01959, partial [Genlise... 110 3e-22
ref|XP_004509953.1| PREDICTED: probable phosphatidylinositol 4-k... 107 2e-21
>ref|XP_002514582.1| inositol or phosphatidylinositol kinase, putative [Ricinus
communis] gi|223546186|gb|EEF47688.1| inositol or
phosphatidylinositol kinase, putative [Ricinus communis]
Length = 647
Score = 127 bits (320), Expect = 1e-27
Identities = 65/102 (63%), Positives = 78/102 (76%)
Frame = -2
Query: 307 EEENGSISVTPPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRS 128
E+ G SV P R+ T+ KLSMSLK+ SLGEK QK KFSG KP+N Y ++SS HRS
Sbjct: 529 EDGKGGFSVLPMPERLPTMSKLSMSLKSTSLGEKKQKCQKFSGSKPENGYYANTSSGHRS 588
Query: 127 ANEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
ANEQLP SVSFV++ADMS++EW +FL+KFQELL P F KRKS
Sbjct: 589 ANEQLPASVSFVELADMSDDEWTLFLEKFQELLYPAFNKRKS 630
>gb|EYU22584.1| hypothetical protein MIMGU_mgv1a002776mg [Mimulus guttatus]
Length = 639
Score = 127 bits (319), Expect = 2e-27
Identities = 68/102 (66%), Positives = 78/102 (76%)
Frame = -2
Query: 307 EEENGSISVTPPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRS 128
E+E G SVT L R T+ LS+SLKN SLGEK++KF KF G K + Y SSSS HRS
Sbjct: 522 EQEKGLASVTA-LLRNPTVTALSVSLKNTSLGEKSKKFPKFFGGKDEGSYAGSSSSGHRS 580
Query: 127 ANEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
ANEQLP S SFVKVADM+EEEWG+FLDKFQ+LL P FA+RKS
Sbjct: 581 ANEQLPASASFVKVADMNEEEWGLFLDKFQDLLNPAFAERKS 622
>ref|XP_006348640.1| PREDICTED: phosphatidylinositol 4-kinase gamma 7-like [Solanum
tuberosum]
Length = 640
Score = 127 bits (319), Expect = 2e-27
Identities = 66/102 (64%), Positives = 76/102 (74%)
Frame = -2
Query: 307 EEENGSISVTPPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRS 128
EEE+ + + P + KLSMSLKNISLG+K QK FSG KP+N YL +SSS HRS
Sbjct: 522 EEEDTNFNYVPAPAKNVAESKLSMSLKNISLGDKNQKHPYFSGTKPENAYLANSSSGHRS 581
Query: 127 ANEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
ANEQLP S SFVK+ADM EEEW +FL+KFQELL P FAKRKS
Sbjct: 582 ANEQLPVSSSFVKLADMGEEEWRLFLEKFQELLYPAFAKRKS 623
>ref|XP_007045737.1| Phosphoinositide 4-kinase gamma 7, gamma 7,ubdk gamma 7,pi4k gamma
7 isoform 1 [Theobroma cacao]
gi|590698519|ref|XP_007045738.1| Phosphoinositide
4-kinase gamma 7, gamma 7,ubdk gamma 7,pi4k gamma 7
isoform 1 [Theobroma cacao] gi|508709672|gb|EOY01569.1|
Phosphoinositide 4-kinase gamma 7, gamma 7,ubdk gamma
7,pi4k gamma 7 isoform 1 [Theobroma cacao]
gi|508709673|gb|EOY01570.1| Phosphoinositide 4-kinase
gamma 7, gamma 7,ubdk gamma 7,pi4k gamma 7 isoform 1
[Theobroma cacao]
Length = 641
Score = 126 bits (317), Expect = 3e-27
Identities = 69/102 (67%), Positives = 78/102 (76%)
Frame = -2
Query: 307 EEENGSISVTPPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRS 128
E E + S L RI TI KLSMSLK+ SLG+K+QK+SKFSG K +N YLTSSS HRS
Sbjct: 524 EVEQENFSAFKALQRIPTISKLSMSLKDTSLGDKSQKYSKFSGTKQENGYLTSSSG-HRS 582
Query: 127 ANEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
ANEQLP SVSFVK+ADM+EEEW FL+KFQELL F KRKS
Sbjct: 583 ANEQLPASVSFVKLADMNEEEWAYFLEKFQELLYAAFEKRKS 624
>ref|XP_004239016.1| PREDICTED: probable phosphatidylinositol 4-kinase type 2-beta
At1g26270-like [Solanum lycopersicum]
Length = 640
Score = 126 bits (317), Expect = 3e-27
Identities = 66/102 (64%), Positives = 77/102 (75%)
Frame = -2
Query: 307 EEENGSISVTPPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRS 128
EEE+ S++ P + KLSMSLKNISLG+K QK FSG KP+N +L +SSS HRS
Sbjct: 522 EEEDTSVNCVPAPAKNVEELKLSMSLKNISLGDKNQKHPYFSGTKPENAHLANSSSGHRS 581
Query: 127 ANEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
ANEQLP S SFVK+ADM EEEW +FL+KFQELL P FAKRKS
Sbjct: 582 ANEQLPVSSSFVKLADMGEEEWRLFLEKFQELLYPAFAKRKS 623
>ref|XP_006363554.1| PREDICTED: phosphatidylinositol 4-kinase gamma 7-like [Solanum
tuberosum]
Length = 640
Score = 126 bits (316), Expect = 4e-27
Identities = 64/102 (62%), Positives = 75/102 (73%)
Frame = -2
Query: 307 EEENGSISVTPPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRS 128
EE+ S + P R + KLSMSLKN SLGEK K+ SG KP+N YL SSSS HRS
Sbjct: 522 EEDEKSFNYVPAPARNIAVSKLSMSLKNTSLGEKKLKYPNISGTKPENGYLASSSSGHRS 581
Query: 127 ANEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
ANEQLP SV+FVKV+DM + EWG+FL+KF+ELL P FAKRKS
Sbjct: 582 ANEQLPASVTFVKVSDMDDVEWGLFLEKFEELLYPAFAKRKS 623
>ref|XP_004237064.1| PREDICTED: probable phosphatidylinositol 4-kinase type 2-beta
At1g26270-like [Solanum lycopersicum]
Length = 640
Score = 124 bits (310), Expect = 2e-26
Identities = 62/101 (61%), Positives = 75/101 (74%)
Frame = -2
Query: 304 EENGSISVTPPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRSA 125
E+ S + P + + KLSMSLKN SLGEK K+ SG KP+N YL SSSS HRSA
Sbjct: 523 EDEKSFNYVPAPAKNIAVSKLSMSLKNTSLGEKKLKYPNISGTKPENGYLASSSSGHRSA 582
Query: 124 NEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
NEQLP SV+FVK++DM++ EWG+FLDKF+ELL P FAKRKS
Sbjct: 583 NEQLPASVTFVKLSDMNDVEWGLFLDKFEELLYPAFAKRKS 623
>ref|XP_004146767.1| PREDICTED: probable phosphatidylinositol 4-kinase type 2-beta
At1g26270-like [Cucumis sativus]
gi|449523902|ref|XP_004168962.1| PREDICTED: probable
phosphatidylinositol 4-kinase type 2-beta At1g26270-like
[Cucumis sativus]
Length = 645
Score = 124 bits (310), Expect = 2e-26
Identities = 64/103 (62%), Positives = 77/103 (74%), Gaps = 1/103 (0%)
Frame = -2
Query: 307 EEENGSISVTPPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYL-TSSSSSHR 131
+E G + RI TI KLSMSLKN SLGEK +K S + G +PDN Y+ T++SS HR
Sbjct: 526 DERGGGFTNLLSSERIPTISKLSMSLKNTSLGEKNKKHSNYFGTRPDNGYMMTNTSSGHR 585
Query: 130 SANEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
SANEQLP SV+FVK+ADM+E+ W +FLDKFQELL P FAKRKS
Sbjct: 586 SANEQLPASVTFVKLADMNEDSWSLFLDKFQELLHPAFAKRKS 628
>ref|XP_006484264.1| PREDICTED: phosphatidylinositol 4-kinase gamma 7-like [Citrus
sinensis]
Length = 644
Score = 123 bits (309), Expect = 2e-26
Identities = 63/102 (61%), Positives = 75/102 (73%)
Frame = -2
Query: 307 EEENGSISVTPPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRS 128
EEE S P RI +I KLSMSLK LG+K QK+ KFSG KP+N L+++SS HRS
Sbjct: 526 EEEQEGFSTLPAPERIPSISKLSMSLKTTCLGDKNQKYQKFSGSKPENGCLSNTSSGHRS 585
Query: 127 ANEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
ANEQLP VSFVK+AD+ E+EW +FL+KFQELL P FA RKS
Sbjct: 586 ANEQLPACVSFVKLADLGEDEWTLFLEKFQELLYPAFAARKS 627
>ref|XP_006437843.1| hypothetical protein CICLE_v10030931mg [Citrus clementina]
gi|557540039|gb|ESR51083.1| hypothetical protein
CICLE_v10030931mg [Citrus clementina]
Length = 644
Score = 123 bits (309), Expect = 2e-26
Identities = 63/102 (61%), Positives = 75/102 (73%)
Frame = -2
Query: 307 EEENGSISVTPPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRS 128
EEE S P RI +I KLSMSLK LG+K QK+ KFSG KP+N L+++SS HRS
Sbjct: 526 EEEQEGFSTLPAPERIPSISKLSMSLKTTCLGDKNQKYQKFSGSKPENGCLSNTSSGHRS 585
Query: 127 ANEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
ANEQLP VSFVK+AD+ E+EW +FL+KFQELL P FA RKS
Sbjct: 586 ANEQLPACVSFVKLADLGEDEWTLFLEKFQELLYPAFAARKS 627
>ref|XP_004135137.1| PREDICTED: probable phosphatidylinositol 4-kinase type 2-beta
At1g26270-like isoform 1 [Cucumis sativus]
gi|449434708|ref|XP_004135138.1| PREDICTED: probable
phosphatidylinositol 4-kinase type 2-beta At1g26270-like
isoform 2 [Cucumis sativus]
Length = 645
Score = 122 bits (306), Expect = 5e-26
Identities = 60/101 (59%), Positives = 73/101 (72%)
Frame = -2
Query: 304 EENGSISVTPPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRSA 125
EE G + P + + KLSMSLKN LG+K QK K+ KP+ Y+T++S+ HRSA
Sbjct: 528 EEQGETASFLPSEPVSVVSKLSMSLKNTILGDKNQKNQKYQVTKPEYGYITNTSAGHRSA 587
Query: 124 NEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
NEQ PPSVSFVK+ DM+EEEW +FLDKF ELLRP FAKRKS
Sbjct: 588 NEQFPPSVSFVKLGDMNEEEWSIFLDKFPELLRPAFAKRKS 628
>gb|EXB44906.1| putative phosphatidylinositol 4-kinase type 2-beta [Morus
notabilis]
Length = 642
Score = 119 bits (297), Expect = 6e-25
Identities = 57/85 (67%), Positives = 71/85 (83%)
Frame = -2
Query: 256 TIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRSANEQLPPSVSFVKVADM 77
TI +SMSLK+ SLGEK QKF KF+G KP+N Y+ ++S+ HRSANEQLP SVSFVK+AD+
Sbjct: 541 TISNISMSLKDTSLGEKKQKFPKFTGSKPENGYMLNTSAGHRSANEQLPASVSFVKLADL 600
Query: 76 SEEEWGVFLDKFQELLRPVFAKRKS 2
SE+EW +FL+KF +LL P FAKRKS
Sbjct: 601 SEDEWTLFLEKFHDLLYPAFAKRKS 625
>gb|ADK78996.1| phosphatidylinositol 4-kinase [Gossypium barbadense]
Length = 641
Score = 118 bits (296), Expect = 7e-25
Identities = 64/102 (62%), Positives = 76/102 (74%)
Frame = -2
Query: 307 EEENGSISVTPPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRS 128
E E S L RI + KLSMS KN SLG+K+QK+SKFSG K +N YL ++SS HRS
Sbjct: 524 ETEQPCFSTFKALERIPSTSKLSMSFKNTSLGDKSQKYSKFSGTKQENGYL-NNSSGHRS 582
Query: 127 ANEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
A EQLP +VSFVK+ADM+EEEW +FL+KFQELL P F KRKS
Sbjct: 583 ATEQLPGNVSFVKLADMNEEEWTLFLEKFQELLYPGFEKRKS 624
>ref|XP_004297194.1| PREDICTED: probable phosphatidylinositol 4-kinase type 2-beta
At1g26270-like [Fragaria vesca subsp. vesca]
Length = 637
Score = 116 bits (290), Expect = 4e-24
Identities = 57/101 (56%), Positives = 77/101 (76%)
Frame = -2
Query: 304 EENGSISVTPPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRSA 125
EE +++ PP I ++ KLSMSLK SLGEK F K+ G K +N+++ ++SS HRSA
Sbjct: 522 EEQQALASLPPSKHIPSVAKLSMSLKTTSLGEKN--FPKYIGAKTENVHMANTSSGHRSA 579
Query: 124 NEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
NEQLP S+SFVK+ADM+E++W +FL+KFQ+LL P FAKRKS
Sbjct: 580 NEQLPASMSFVKLADMNEDDWTLFLEKFQDLLYPAFAKRKS 620
>ref|XP_002316001.1| phosphatidylinositol 3- and 4-kinase family protein [Populus
trichocarpa] gi|222865041|gb|EEF02172.1|
phosphatidylinositol 3- and 4-kinase family protein
[Populus trichocarpa]
Length = 640
Score = 116 bits (290), Expect = 4e-24
Identities = 61/102 (59%), Positives = 76/102 (74%)
Frame = -2
Query: 307 EEENGSISVTPPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRS 128
EEE + L ++ TI KLSMSLK+ +LG+K QK S G KP+N L++ SS HRS
Sbjct: 525 EEEQEGFAALRTLEKLPTISKLSMSLKSTTLGDKNQKLS---GTKPENGCLSNRSSGHRS 581
Query: 127 ANEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
ANEQLP S+SFV++ADM+EEEW +FL+KFQELL P FAKRKS
Sbjct: 582 ANEQLPASISFVELADMTEEEWTLFLEKFQELLYPAFAKRKS 623
>ref|XP_002267025.1| PREDICTED: probable phosphatidylinositol 4-kinase type 2-beta
At1g26270 isoform 1 [Vitis vinifera]
gi|147838249|emb|CAN76598.1| hypothetical protein
VITISV_005885 [Vitis vinifera]
Length = 640
Score = 116 bits (290), Expect = 4e-24
Identities = 61/102 (59%), Positives = 76/102 (74%)
Frame = -2
Query: 307 EEENGSISVTPPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRS 128
E E + P + TI KLSMSLK+ SLGEK+QK+ + KP++ YL ++SS HRS
Sbjct: 525 EGEQEDFAGIPTPAEVSTISKLSMSLKDTSLGEKSQKYLE---AKPESSYLANTSSGHRS 581
Query: 127 ANEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
ANEQLP S+SFVK+ADM+EEEW +FL+KFQELL P FAKRKS
Sbjct: 582 ANEQLPTSMSFVKLADMNEEEWVLFLEKFQELLYPAFAKRKS 623
>emb|CBI27403.3| unnamed protein product [Vitis vinifera]
Length = 392
Score = 114 bits (284), Expect = 2e-23
Identities = 58/87 (66%), Positives = 72/87 (82%)
Frame = -2
Query: 262 IHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRSANEQLPPSVSFVKVA 83
+ TI KLSMSLK+ SLGEK+QK+ + KP++ YL ++SS HRSANEQLP S+SFVK+A
Sbjct: 292 VSTISKLSMSLKDTSLGEKSQKYLE---AKPESSYLANTSSGHRSANEQLPTSMSFVKLA 348
Query: 82 DMSEEEWGVFLDKFQELLRPVFAKRKS 2
DM+EEEW +FL+KFQELL P FAKRKS
Sbjct: 349 DMNEEEWVLFLEKFQELLYPAFAKRKS 375
>ref|XP_002311415.2| phosphatidylinositol 3- and 4-kinase family protein [Populus
trichocarpa] gi|550332833|gb|EEE88782.2|
phosphatidylinositol 3- and 4-kinase family protein
[Populus trichocarpa]
Length = 642
Score = 112 bits (279), Expect = 7e-23
Identities = 61/103 (59%), Positives = 76/103 (73%), Gaps = 1/103 (0%)
Frame = -2
Query: 307 EEENGSISVTPPLTRIHTIPKLSMSLKN-ISLGEKTQKFSKFSGIKPDNIYLTSSSSSHR 131
EEE + + TI KLSMSLK+ +LG+K QKFS G KP+N YL++ SS HR
Sbjct: 524 EEEQECFTALRTPEKFPTISKLSMSLKSSTTLGDKNQKFS---GTKPENGYLSNRSSGHR 580
Query: 130 SANEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
SANEQLP S+SFV++ADM+EEEW +F++KFQELL P FAKRKS
Sbjct: 581 SANEQLPASMSFVELADMTEEEWTLFMEKFQELLYPAFAKRKS 623
>gb|EPS72797.1| hypothetical protein M569_01959, partial [Genlisea aurea]
Length = 643
Score = 110 bits (274), Expect = 3e-22
Identities = 62/102 (60%), Positives = 72/102 (70%)
Frame = -2
Query: 307 EEENGSISVTPPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRS 128
EEE +S+ T I IPKLSMSL N LGEK QK KFS + + +SSSSSHRS
Sbjct: 528 EEEKAWLSIARSPTEI--IPKLSMSLSNTILGEKKQKKPKFSAVSLVSSS-SSSSSSHRS 584
Query: 127 ANEQLPPSVSFVKVADMSEEEWGVFLDKFQELLRPVFAKRKS 2
AN+ LPPSVSFVKV DM EEEWG+FLD+F EL+ P F KRK+
Sbjct: 585 ANDPLPPSVSFVKVVDMKEEEWGLFLDRFVELVTPAFEKRKA 626
>ref|XP_004509953.1| PREDICTED: probable phosphatidylinositol 4-kinase type 2-beta
At1g26270-like [Cicer arietinum]
Length = 639
Score = 107 bits (267), Expect = 2e-21
Identities = 55/91 (60%), Positives = 66/91 (72%)
Frame = -2
Query: 277 PPLTRIHTIPKLSMSLKNISLGEKTQKFSKFSGIKPDNIYLTSSSSSHRSANEQLPPSVS 98
PP ++ T+ KLSMSLKN L EK+QK SG DN Y ++SS H+SANEQL S S
Sbjct: 531 PPQEKVPTVSKLSMSLKNTMLSEKSQKHQNHSGGIADNGYYGNTSSGHKSANEQLLASTS 590
Query: 97 FVKVADMSEEEWGVFLDKFQELLRPVFAKRK 5
FVK+ADM+E+EW FL+KFQELL P FAKRK
Sbjct: 591 FVKLADMTEDEWITFLEKFQELLYPAFAKRK 621