BLASTX nr result
ID: Mentha22_contig00020453
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Mentha22_contig00020453
(380 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EYU33240.1| hypothetical protein MIMGU_mgv1a000222mg [Mimulus... 102 6e-20
ref|XP_004170301.1| PREDICTED: uncharacterized LOC101213309, par... 100 3e-19
ref|XP_004149319.1| PREDICTED: uncharacterized protein LOC101213... 100 3e-19
ref|XP_006358496.1| PREDICTED: uncharacterized protein LOC102597... 100 4e-19
ref|XP_006358495.1| PREDICTED: uncharacterized protein LOC102597... 100 4e-19
ref|XP_004230382.1| PREDICTED: uncharacterized protein LOC101246... 99 5e-19
ref|XP_004494493.1| PREDICTED: uncharacterized protein LOC101494... 98 1e-18
ref|XP_007200336.1| hypothetical protein PRUPE_ppa000254mg [Prun... 98 1e-18
ref|XP_003521631.1| PREDICTED: uncharacterized protein LOC100777... 98 1e-18
ref|XP_006478516.1| PREDICTED: uncharacterized protein LOC102607... 97 3e-18
ref|XP_006441965.1| hypothetical protein CICLE_v10018511mg [Citr... 97 3e-18
gb|AAZ31064.1| WD-40 repeat family protein [Medicago sativa] 96 5e-18
ref|XP_007014610.1| Transducin/WD40 repeat-like superfamily prot... 96 7e-18
ref|XP_007014609.1| Transducin/WD40 repeat-like superfamily prot... 96 7e-18
ref|XP_007014608.1| Transducin/WD40 repeat-like superfamily prot... 96 7e-18
ref|XP_007014607.1| Transducin/WD40 repeat-like superfamily prot... 96 7e-18
ref|XP_007014606.1| Transducin/WD40 repeat-like superfamily prot... 96 7e-18
ref|XP_007014605.1| Transducin/WD40 repeat-like superfamily prot... 96 7e-18
ref|XP_002309040.1| WD-40 repeat family protein [Populus trichoc... 94 2e-17
ref|XP_002521175.1| conserved hypothetical protein [Ricinus comm... 94 3e-17
>gb|EYU33240.1| hypothetical protein MIMGU_mgv1a000222mg [Mimulus guttatus]
Length = 1413
Score = 102 bits (254), Expect = 6e-20
Identities = 60/111 (54%), Positives = 66/111 (59%), Gaps = 10/111 (9%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRFA-------TSIQXXX 235
VQVRILLDDGTSNILMRSVG+RSEPV YRT RR + ++IQ
Sbjct: 611 VQVRILLDDGTSNILMRSVGNRSEPVSGLHGGALLGVAYRTSRRISPVTATAISTIQSMP 670
Query: 236 XXXXXXXXXXXXXXXDDG---QKLNADALPHNFQLYSWETFQPVGGLLPQP 379
DDG QK +A+A P NFQLYSWETFQPVGGLLPQP
Sbjct: 671 LSGFSGSSVSSFSTMDDGYSSQKSSAEAAPQNFQLYSWETFQPVGGLLPQP 721
>ref|XP_004170301.1| PREDICTED: uncharacterized LOC101213309, partial [Cucumis sativus]
Length = 844
Score = 100 bits (248), Expect = 3e-19
Identities = 59/108 (54%), Positives = 63/108 (58%), Gaps = 7/108 (6%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRF----ATSIQXXXXXX 244
VQVRILLDDGTSNILMRS+GSRSEPV YRT RR AT+I
Sbjct: 348 VQVRILLDDGTSNILMRSIGSRSEPVVGLHGGALLGVAYRTSRRISPVAATAISTMPLSG 407
Query: 245 XXXXXXXXXXXXDDG---QKLNADALPHNFQLYSWETFQPVGGLLPQP 379
DDG K +A+ P NFQLYSWETFQPVGGLLPQP
Sbjct: 408 FGNSGVSSFTSFDDGFSSLKSSAETTPPNFQLYSWETFQPVGGLLPQP 455
>ref|XP_004149319.1| PREDICTED: uncharacterized protein LOC101213309 [Cucumis sativus]
Length = 1343
Score = 100 bits (248), Expect = 3e-19
Identities = 59/108 (54%), Positives = 63/108 (58%), Gaps = 7/108 (6%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRF----ATSIQXXXXXX 244
VQVRILLDDGTSNILMRS+GSRSEPV YRT RR AT+I
Sbjct: 575 VQVRILLDDGTSNILMRSIGSRSEPVVGLHGGALLGVAYRTSRRISPVAATAISTMPLSG 634
Query: 245 XXXXXXXXXXXXDDG---QKLNADALPHNFQLYSWETFQPVGGLLPQP 379
DDG K +A+ P NFQLYSWETFQPVGGLLPQP
Sbjct: 635 FGNSGVSSFTSFDDGFSSLKSSAETTPPNFQLYSWETFQPVGGLLPQP 682
>ref|XP_006358496.1| PREDICTED: uncharacterized protein LOC102597779 isoform X2 [Solanum
tuberosum]
Length = 1395
Score = 99.8 bits (247), Expect = 4e-19
Identities = 58/109 (53%), Positives = 65/109 (59%), Gaps = 8/109 (7%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRFA-------TSIQXXX 235
VQVRILLDDGTSN+LM+SVGSRSEPV YRT RR + ++IQ
Sbjct: 576 VQVRILLDDGTSNVLMKSVGSRSEPVIGLHGGALLGVAYRTSRRVSAAAATAISTIQSMP 635
Query: 236 XXXXXXXXXXXXXXXDDG-QKLNADALPHNFQLYSWETFQPVGGLLPQP 379
+DG QK A+A P NFQLYSWETFQPVGGLLPQP
Sbjct: 636 LSGYGGSSVSSFSTMEDGSQKSAAEAAPQNFQLYSWETFQPVGGLLPQP 684
>ref|XP_006358495.1| PREDICTED: uncharacterized protein LOC102597779 isoform X1 [Solanum
tuberosum]
Length = 1411
Score = 99.8 bits (247), Expect = 4e-19
Identities = 58/109 (53%), Positives = 65/109 (59%), Gaps = 8/109 (7%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRFA-------TSIQXXX 235
VQVRILLDDGTSN+LM+SVGSRSEPV YRT RR + ++IQ
Sbjct: 576 VQVRILLDDGTSNVLMKSVGSRSEPVIGLHGGALLGVAYRTSRRVSAAAATAISTIQSMP 635
Query: 236 XXXXXXXXXXXXXXXDDG-QKLNADALPHNFQLYSWETFQPVGGLLPQP 379
+DG QK A+A P NFQLYSWETFQPVGGLLPQP
Sbjct: 636 LSGYGGSSVSSFSTMEDGSQKSAAEAAPQNFQLYSWETFQPVGGLLPQP 684
>ref|XP_004230382.1| PREDICTED: uncharacterized protein LOC101246184 [Solanum
lycopersicum]
Length = 1400
Score = 99.4 bits (246), Expect = 5e-19
Identities = 58/109 (53%), Positives = 65/109 (59%), Gaps = 8/109 (7%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRFA-------TSIQXXX 235
VQVRILLDDGTSN+LM+SVGSRSEPV YRT RR + ++IQ
Sbjct: 576 VQVRILLDDGTSNVLMKSVGSRSEPVIGLHGGALLGVAYRTSRRVSAAAATAISTIQSMP 635
Query: 236 XXXXXXXXXXXXXXXDDG-QKLNADALPHNFQLYSWETFQPVGGLLPQP 379
+DG QK A+A P NFQLYSWETFQPVGGLLPQP
Sbjct: 636 LSGYGGSSVSSFSTMEDGSQKSVAEAAPQNFQLYSWETFQPVGGLLPQP 684
>ref|XP_004494493.1| PREDICTED: uncharacterized protein LOC101494449 [Cicer arietinum]
Length = 1329
Score = 98.2 bits (243), Expect = 1e-18
Identities = 58/111 (52%), Positives = 64/111 (57%), Gaps = 10/111 (9%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRFA-------TSIQXXX 235
VQVRILLDDGTSNILMRSVG+RSEPV YRT RR + ++IQ
Sbjct: 573 VQVRILLDDGTSNILMRSVGARSEPVIGLHGGALLGVAYRTSRRISPIAATAISTIQSMP 632
Query: 236 XXXXXXXXXXXXXXXDDG---QKLNADALPHNFQLYSWETFQPVGGLLPQP 379
DDG + A+A P NFQLYSWETFQPVGGLLPQP
Sbjct: 633 LSGYGSSGLSSFTTYDDGFSSHRSPAEAAPQNFQLYSWETFQPVGGLLPQP 683
>ref|XP_007200336.1| hypothetical protein PRUPE_ppa000254mg [Prunus persica]
gi|462395736|gb|EMJ01535.1| hypothetical protein
PRUPE_ppa000254mg [Prunus persica]
Length = 1384
Score = 97.8 bits (242), Expect = 1e-18
Identities = 56/111 (50%), Positives = 64/111 (57%), Gaps = 10/111 (9%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRFA-------TSIQXXX 235
VQVRILLDDGTSNILMRS+G RSEPV YRT RR + ++IQ
Sbjct: 571 VQVRILLDDGTSNILMRSIGGRSEPVIGLHGGALLGVAYRTSRRISPVAATAISTIQSMP 630
Query: 236 XXXXXXXXXXXXXXXDDG---QKLNADALPHNFQLYSWETFQPVGGLLPQP 379
DDG + +A+A P NFQLYSWETFQPVGGL+PQP
Sbjct: 631 LSGFGSGGHSSFSTFDDGFSSHRSSAEAAPQNFQLYSWETFQPVGGLIPQP 681
>ref|XP_003521631.1| PREDICTED: uncharacterized protein LOC100777567 [Glycine max]
Length = 1352
Score = 97.8 bits (242), Expect = 1e-18
Identities = 58/111 (52%), Positives = 64/111 (57%), Gaps = 10/111 (9%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRFA-------TSIQXXX 235
VQVRILLDDGTSNILMRSVG+RSEPV YRT RR + ++IQ
Sbjct: 572 VQVRILLDDGTSNILMRSVGARSEPVIGLHGGALLGVAYRTSRRVSPIAATAISTIQSMP 631
Query: 236 XXXXXXXXXXXXXXXDDG---QKLNADALPHNFQLYSWETFQPVGGLLPQP 379
DDG Q+ +A P NFQLYSWETFQPVGGLLPQP
Sbjct: 632 LSGYGSSGVSSFSTYDDGFSSQRPPTEAAPQNFQLYSWETFQPVGGLLPQP 682
>ref|XP_006478516.1| PREDICTED: uncharacterized protein LOC102607648 [Citrus sinensis]
Length = 1352
Score = 96.7 bits (239), Expect = 3e-18
Identities = 57/111 (51%), Positives = 62/111 (55%), Gaps = 10/111 (9%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRFA-------TSIQXXX 235
VQ RILLDDGTSNILMRS+G SEPV YRT RR + ++IQ
Sbjct: 577 VQARILLDDGTSNILMRSIGGSSEPVIGLHGGALLGVAYRTSRRISPIAATAISTIQSMP 636
Query: 236 XXXXXXXXXXXXXXXDDG---QKLNADALPHNFQLYSWETFQPVGGLLPQP 379
DDG QK A+A P NFQLYSWETFQPVGGLLPQP
Sbjct: 637 LSGFGSSGLSSFTTFDDGFSSQKSPAEAAPQNFQLYSWETFQPVGGLLPQP 687
>ref|XP_006441965.1| hypothetical protein CICLE_v10018511mg [Citrus clementina]
gi|557544227|gb|ESR55205.1| hypothetical protein
CICLE_v10018511mg [Citrus clementina]
Length = 1390
Score = 96.7 bits (239), Expect = 3e-18
Identities = 57/111 (51%), Positives = 62/111 (55%), Gaps = 10/111 (9%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRFA-------TSIQXXX 235
VQ RILLDDGTSNILMRS+G SEPV YRT RR + ++IQ
Sbjct: 615 VQARILLDDGTSNILMRSIGGSSEPVIGLHGGALLGVAYRTSRRISPIAATAISTIQSMP 674
Query: 236 XXXXXXXXXXXXXXXDDG---QKLNADALPHNFQLYSWETFQPVGGLLPQP 379
DDG QK A+A P NFQLYSWETFQPVGGLLPQP
Sbjct: 675 LSGFGSSGLSSFTTFDDGFSSQKSPAEAAPQNFQLYSWETFQPVGGLLPQP 725
>gb|AAZ31064.1| WD-40 repeat family protein [Medicago sativa]
Length = 417
Score = 95.9 bits (237), Expect = 5e-18
Identities = 56/111 (50%), Positives = 63/111 (56%), Gaps = 10/111 (9%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRFA-------TSIQXXX 235
VQVRI+LDDGTSN+LMRSVG+RSEPV YRT RR ++IQ
Sbjct: 102 VQVRIMLDDGTSNMLMRSVGARSEPVIGLHGGALLGVAYRTSRRIGPIAATAISTIQSMP 161
Query: 236 XXXXXXXXXXXXXXXDDGQKLN---ADALPHNFQLYSWETFQPVGGLLPQP 379
DDG N A+A+P NFQLYSWETFQPVG LLPQP
Sbjct: 162 LSGYGSSGLSSFTTYDDGLSSNRSPAEAVPQNFQLYSWETFQPVGALLPQP 212
>ref|XP_007014610.1| Transducin/WD40 repeat-like superfamily protein isoform 6
[Theobroma cacao] gi|508784973|gb|EOY32229.1|
Transducin/WD40 repeat-like superfamily protein isoform
6 [Theobroma cacao]
Length = 973
Score = 95.5 bits (236), Expect = 7e-18
Identities = 58/109 (53%), Positives = 64/109 (58%), Gaps = 8/109 (7%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRF----ATSIQ-XXXXX 241
VQVRILLDDGTSNILMRS+GSRSEPV YRT RR AT+I
Sbjct: 575 VQVRILLDDGTSNILMRSIGSRSEPVIGLHGGALLGVAYRTSRRISPGSATAISTIQSMP 634
Query: 242 XXXXXXXXXXXXXDDGQKLN---ADALPHNFQLYSWETFQPVGGLLPQP 379
DDG N ++A+P NFQL+SWETFQPVGGLLPQP
Sbjct: 635 LSGFGSSGSFAAFDDGFSSNRSPSEAVPQNFQLFSWETFQPVGGLLPQP 683
>ref|XP_007014609.1| Transducin/WD40 repeat-like superfamily protein isoform 5
[Theobroma cacao] gi|508784972|gb|EOY32228.1|
Transducin/WD40 repeat-like superfamily protein isoform
5 [Theobroma cacao]
Length = 969
Score = 95.5 bits (236), Expect = 7e-18
Identities = 58/109 (53%), Positives = 64/109 (58%), Gaps = 8/109 (7%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRF----ATSIQ-XXXXX 241
VQVRILLDDGTSNILMRS+GSRSEPV YRT RR AT+I
Sbjct: 575 VQVRILLDDGTSNILMRSIGSRSEPVIGLHGGALLGVAYRTSRRISPGSATAISTIQSMP 634
Query: 242 XXXXXXXXXXXXXDDGQKLN---ADALPHNFQLYSWETFQPVGGLLPQP 379
DDG N ++A+P NFQL+SWETFQPVGGLLPQP
Sbjct: 635 LSGFGSSGSFAAFDDGFSSNRSPSEAVPQNFQLFSWETFQPVGGLLPQP 683
>ref|XP_007014608.1| Transducin/WD40 repeat-like superfamily protein isoform 4, partial
[Theobroma cacao] gi|508784971|gb|EOY32227.1|
Transducin/WD40 repeat-like superfamily protein isoform
4, partial [Theobroma cacao]
Length = 1050
Score = 95.5 bits (236), Expect = 7e-18
Identities = 58/109 (53%), Positives = 64/109 (58%), Gaps = 8/109 (7%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRF----ATSIQ-XXXXX 241
VQVRILLDDGTSNILMRS+GSRSEPV YRT RR AT+I
Sbjct: 357 VQVRILLDDGTSNILMRSIGSRSEPVIGLHGGALLGVAYRTSRRISPGSATAISTIQSMP 416
Query: 242 XXXXXXXXXXXXXDDGQKLN---ADALPHNFQLYSWETFQPVGGLLPQP 379
DDG N ++A+P NFQL+SWETFQPVGGLLPQP
Sbjct: 417 LSGFGSSGSFAAFDDGFSSNRSPSEAVPQNFQLFSWETFQPVGGLLPQP 465
>ref|XP_007014607.1| Transducin/WD40 repeat-like superfamily protein isoform 3
[Theobroma cacao] gi|508784970|gb|EOY32226.1|
Transducin/WD40 repeat-like superfamily protein isoform
3 [Theobroma cacao]
Length = 1351
Score = 95.5 bits (236), Expect = 7e-18
Identities = 58/109 (53%), Positives = 64/109 (58%), Gaps = 8/109 (7%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRF----ATSIQ-XXXXX 241
VQVRILLDDGTSNILMRS+GSRSEPV YRT RR AT+I
Sbjct: 575 VQVRILLDDGTSNILMRSIGSRSEPVIGLHGGALLGVAYRTSRRISPGSATAISTIQSMP 634
Query: 242 XXXXXXXXXXXXXDDGQKLN---ADALPHNFQLYSWETFQPVGGLLPQP 379
DDG N ++A+P NFQL+SWETFQPVGGLLPQP
Sbjct: 635 LSGFGSSGSFAAFDDGFSSNRSPSEAVPQNFQLFSWETFQPVGGLLPQP 683
>ref|XP_007014606.1| Transducin/WD40 repeat-like superfamily protein isoform 2
[Theobroma cacao] gi|508784969|gb|EOY32225.1|
Transducin/WD40 repeat-like superfamily protein isoform
2 [Theobroma cacao]
Length = 1351
Score = 95.5 bits (236), Expect = 7e-18
Identities = 58/109 (53%), Positives = 64/109 (58%), Gaps = 8/109 (7%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRF----ATSIQ-XXXXX 241
VQVRILLDDGTSNILMRS+GSRSEPV YRT RR AT+I
Sbjct: 575 VQVRILLDDGTSNILMRSIGSRSEPVIGLHGGALLGVAYRTSRRISPGSATAISTIQSMP 634
Query: 242 XXXXXXXXXXXXXDDGQKLN---ADALPHNFQLYSWETFQPVGGLLPQP 379
DDG N ++A+P NFQL+SWETFQPVGGLLPQP
Sbjct: 635 LSGFGSSGSFAAFDDGFSSNRSPSEAVPQNFQLFSWETFQPVGGLLPQP 683
>ref|XP_007014605.1| Transducin/WD40 repeat-like superfamily protein isoform 1
[Theobroma cacao] gi|508784968|gb|EOY32224.1|
Transducin/WD40 repeat-like superfamily protein isoform
1 [Theobroma cacao]
Length = 1259
Score = 95.5 bits (236), Expect = 7e-18
Identities = 58/109 (53%), Positives = 64/109 (58%), Gaps = 8/109 (7%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRF----ATSIQ-XXXXX 241
VQVRILLDDGTSNILMRS+GSRSEPV YRT RR AT+I
Sbjct: 575 VQVRILLDDGTSNILMRSIGSRSEPVIGLHGGALLGVAYRTSRRISPGSATAISTIQSMP 634
Query: 242 XXXXXXXXXXXXXDDGQKLN---ADALPHNFQLYSWETFQPVGGLLPQP 379
DDG N ++A+P NFQL+SWETFQPVGGLLPQP
Sbjct: 635 LSGFGSSGSFAAFDDGFSSNRSPSEAVPQNFQLFSWETFQPVGGLLPQP 683
>ref|XP_002309040.1| WD-40 repeat family protein [Populus trichocarpa]
gi|222855016|gb|EEE92563.1| WD-40 repeat family protein
[Populus trichocarpa]
Length = 1464
Score = 94.0 bits (232), Expect = 2e-17
Identities = 56/111 (50%), Positives = 61/111 (54%), Gaps = 10/111 (9%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRFA-------TSIQXXX 235
VQVRILLDDGTSNILMRS+ RSEPV YRT RR + ++IQ
Sbjct: 645 VQVRILLDDGTSNILMRSIDGRSEPVIGLHGGALLGVAYRTSRRISPVAATAISTIQSMP 704
Query: 236 XXXXXXXXXXXXXXXDDG---QKLNADALPHNFQLYSWETFQPVGGLLPQP 379
DDG K A+A P NFQLYSWETFQPVGGLLP P
Sbjct: 705 LSGFGSGGLSSFTTLDDGFNSHKSPAEAAPQNFQLYSWETFQPVGGLLPHP 755
>ref|XP_002521175.1| conserved hypothetical protein [Ricinus communis]
gi|223539622|gb|EEF41206.1| conserved hypothetical
protein [Ricinus communis]
Length = 1330
Score = 93.6 bits (231), Expect = 3e-17
Identities = 54/111 (48%), Positives = 64/111 (57%), Gaps = 10/111 (9%)
Frame = +2
Query: 77 VQVRILLDDGTSNILMRSVGSRSEPVXXXXXXXXXXXXYRTCRRFA-------TSIQXXX 235
VQVRILL+DGTSNILMRS+GSRSEPV YRT RR + ++IQ
Sbjct: 576 VQVRILLEDGTSNILMRSIGSRSEPVIGLHGGALLGVAYRTSRRVSPIAATAISTIQSMP 635
Query: 236 XXXXXXXXXXXXXXXDDG---QKLNADALPHNFQLYSWETFQPVGGLLPQP 379
+DG Q+ +A P NF+LYSWETF+PVGGLLPQP
Sbjct: 636 LSGFGGSGVSSFSTFEDGFSSQRSATEAAPQNFELYSWETFEPVGGLLPQP 686