BLASTX nr result
ID: Mentha22_contig00014761
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Mentha22_contig00014761
(629 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AGW27193.1| 4-coumarate:coenzyme A ligase 3 [Salvia miltiorrh... 182 9e-44
dbj|BAJ22963.1| 4-coumarate-CoA ligase [Agastache foeniculum] 177 2e-42
gb|ADO16242.1| 4-coumarate-CoA ligase [Ocimum tenuiflorum] 175 9e-42
gb|AAT02218.1| 4-coumarate-CoA ligase [Agastache rugosa] 175 1e-41
gb|AGP02119.1| 4-coumarate:CoA ligase [Ocimum basilicum] 174 1e-41
gb|EYU19740.1| hypothetical protein MIMGU_mgv1a003643mg [Mimulus... 174 2e-41
gb|EYU45376.1| hypothetical protein MIMGU_mgv1a003734mg [Mimulus... 172 6e-41
gb|AEX32786.1| coumaroyl-CoA ligase [Vitis vinifera] 172 7e-41
emb|CBI26520.3| unnamed protein product [Vitis vinifera] 172 7e-41
ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis ... 172 7e-41
gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum] 172 1e-40
gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium] 171 1e-40
dbj|BAA08366.2| 4-coumarate:CoA ligase [Lithospermum erythrorhizon] 171 2e-40
gb|AHL44980.1| 4-coumarate:coenzyme A ligase 4 [Fraxinus mandshu... 170 3e-40
gb|EPS66025.1| hypothetical protein M569_08750, partial [Genlise... 170 4e-40
ref|XP_004511423.1| PREDICTED: 4-coumarate--CoA ligase 2-like is... 168 1e-39
ref|XP_006443197.1| hypothetical protein CICLE_v10019532mg [Citr... 168 1e-39
gb|AGW16013.1| 4-coumarate:coenzyme A ligase 1 [Pueraria montana... 168 1e-39
ref|XP_004307864.1| PREDICTED: LOW QUALITY PROTEIN: 4-coumarate-... 168 1e-39
ref|XP_007219508.1| hypothetical protein PRUPE_ppa022401mg, part... 168 1e-39
>gb|AGW27193.1| 4-coumarate:coenzyme A ligase 3 [Salvia miltiorrhiza]
Length = 569
Score = 182 bits (461), Expect = 9e-44
Identities = 92/109 (84%), Positives = 96/109 (88%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GDIGY KELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV
Sbjct: 444 GDIGYVDDDDDVFIVDRVKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 503
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVVPS+G+ELTEEAVKEFISKQVVFYKRLHKVYF+HAIPKSP+GKI
Sbjct: 504 PVAFVVPSNGSELTEEAVKEFISKQVVFYKRLHKVYFVHAIPKSPAGKI 552
>dbj|BAJ22963.1| 4-coumarate-CoA ligase [Agastache foeniculum]
Length = 183
Score = 177 bits (449), Expect = 2e-42
Identities = 89/109 (81%), Positives = 95/109 (87%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GDIGY KELIKFKGFQVPPAELEALLISH QISDAAVVPQKD+AAGEV
Sbjct: 66 GDIGYVDEDDDVFIVDRVKELIKFKGFQVPPAELEALLISHSQISDAAVVPQKDDAAGEV 125
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVVP++G+ELTEEAVKEF+SKQVVFYKRLHKVYF+HAIPKSPSGKI
Sbjct: 126 PVAFVVPANGSELTEEAVKEFVSKQVVFYKRLHKVYFVHAIPKSPSGKI 174
>gb|ADO16242.1| 4-coumarate-CoA ligase [Ocimum tenuiflorum]
Length = 567
Score = 175 bits (444), Expect = 9e-42
Identities = 89/109 (81%), Positives = 94/109 (86%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GDIG+ KELIKFKGFQVPPAELEALLISH QISDAAVVPQKDEAAGEV
Sbjct: 443 GDIGFVDEDDDIFIVDRVKELIKFKGFQVPPAELEALLISHSQISDAAVVPQKDEAAGEV 502
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVVP++G ELTEEAVKEFISKQVVFYKRLHKV+F+HAIPKSPSGKI
Sbjct: 503 PVAFVVPAAGCELTEEAVKEFISKQVVFYKRLHKVFFVHAIPKSPSGKI 551
>gb|AAT02218.1| 4-coumarate-CoA ligase [Agastache rugosa]
Length = 553
Score = 175 bits (443), Expect = 1e-41
Identities = 88/109 (80%), Positives = 94/109 (86%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GDIGY KELIKFKGFQVPPAELEALLISH QI DAAVVPQKD+AAGEV
Sbjct: 445 GDIGYVDEDDDVFIVDRVKELIKFKGFQVPPAELEALLISHSQIFDAAVVPQKDDAAGEV 504
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVVP++G+ELTEEAVKEF+SKQVVFYKRLHKVYF+HAIPKSPSGKI
Sbjct: 505 PVAFVVPANGSELTEEAVKEFVSKQVVFYKRLHKVYFVHAIPKSPSGKI 553
>gb|AGP02119.1| 4-coumarate:CoA ligase [Ocimum basilicum]
Length = 567
Score = 174 bits (442), Expect = 1e-41
Identities = 88/109 (80%), Positives = 93/109 (85%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GDIG+ KELIKFKGFQVPPAE+EALLISH QI+DAAVVPQKDEAAGEV
Sbjct: 443 GDIGFVDDDDDVFIVDRVKELIKFKGFQVPPAEMEALLISHSQITDAAVVPQKDEAAGEV 502
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVVP+ G ELTEEAVKEFISKQVVFYKRLHKVYF+HAIPKSPSGKI
Sbjct: 503 PVAFVVPAKGCELTEEAVKEFISKQVVFYKRLHKVYFVHAIPKSPSGKI 551
>gb|EYU19740.1| hypothetical protein MIMGU_mgv1a003643mg [Mimulus guttatus]
Length = 572
Score = 174 bits (441), Expect = 2e-41
Identities = 87/109 (79%), Positives = 93/109 (85%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GDIG+ KELIKFKGFQVPPAELEALLISHP I+DAAVVPQKDEAAGEV
Sbjct: 447 GDIGFVDDDDDVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEAAGEV 506
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVVPS+G ELTEE VKEF+SKQVVFYKRLHKVYF+HAIPKSP+GKI
Sbjct: 507 PVAFVVPSNGFELTEETVKEFVSKQVVFYKRLHKVYFVHAIPKSPAGKI 555
>gb|EYU45376.1| hypothetical protein MIMGU_mgv1a003734mg [Mimulus guttatus]
Length = 567
Score = 172 bits (437), Expect = 6e-41
Identities = 87/109 (79%), Positives = 93/109 (85%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GDIGY KELIKFKGFQVPPAELEALL+SHP I+DAAVVPQKDEAAGEV
Sbjct: 446 GDIGYVDDDDDVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDEAAGEV 505
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVV S+G ELTEEAVK+FISKQVVFYKRLHKVYF+HAIPKSP+GKI
Sbjct: 506 PVAFVVRSNGFELTEEAVKDFISKQVVFYKRLHKVYFVHAIPKSPAGKI 554
>gb|AEX32786.1| coumaroyl-CoA ligase [Vitis vinifera]
Length = 570
Score = 172 bits (436), Expect = 7e-41
Identities = 87/109 (79%), Positives = 92/109 (84%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GDIGY KELIKFKGFQVPPAELEALL+SHP I+DAAVVPQKD+ AGEV
Sbjct: 445 GDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDDVAGEV 504
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVV S+G ELTEEAVKEFISKQVVFYKRLHKVYF+HAIPKSPSGKI
Sbjct: 505 PVAFVVRSNGFELTEEAVKEFISKQVVFYKRLHKVYFVHAIPKSPSGKI 553
>emb|CBI26520.3| unnamed protein product [Vitis vinifera]
Length = 512
Score = 172 bits (436), Expect = 7e-41
Identities = 87/109 (79%), Positives = 92/109 (84%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GDIGY KELIKFKGFQVPPAELEALL+SHP I+DAAVVPQKD+ AGEV
Sbjct: 387 GDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDDVAGEV 446
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVV S+G ELTEEAVKEFISKQVVFYKRLHKVYF+HAIPKSPSGKI
Sbjct: 447 PVAFVVRSNGFELTEEAVKEFISKQVVFYKRLHKVYFVHAIPKSPSGKI 495
>ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis vinifera]
Length = 570
Score = 172 bits (436), Expect = 7e-41
Identities = 87/109 (79%), Positives = 92/109 (84%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GDIGY KELIKFKGFQVPPAELEALL+SHP I+DAAVVPQKD+ AGEV
Sbjct: 445 GDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDDVAGEV 504
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVV S+G ELTEEAVKEFISKQVVFYKRLHKVYF+HAIPKSPSGKI
Sbjct: 505 PVAFVVRSNGFELTEEAVKEFISKQVVFYKRLHKVYFVHAIPKSPSGKI 553
>gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]
Length = 565
Score = 172 bits (435), Expect = 1e-40
Identities = 88/109 (80%), Positives = 92/109 (84%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GD+GY KELIKFKGFQVPPAELEALLISHP I+DAAVVPQKDEAAGEV
Sbjct: 442 GDVGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEALLISHPSIADAAVVPQKDEAAGEV 501
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVV SSG ELTEEAVKEFI+KQVVFYKRLHKVYF+HAIPKS SGKI
Sbjct: 502 PVAFVVRSSGFELTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSASGKI 550
>gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]
Length = 604
Score = 171 bits (434), Expect = 1e-40
Identities = 85/109 (77%), Positives = 93/109 (85%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GD+GY KELIKFKGFQVPPAELE+LL+SHP I+DAAVVPQKDEAAGEV
Sbjct: 480 GDVGYVDDDEEVFIVDRVKELIKFKGFQVPPAELESLLVSHPSIADAAVVPQKDEAAGEV 539
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVV S+G +LTEEAVKEFI+KQVVFYKRLHKVYF+HAIPKSPSGKI
Sbjct: 540 PVAFVVRSNGLQLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKI 588
>dbj|BAA08366.2| 4-coumarate:CoA ligase [Lithospermum erythrorhizon]
Length = 585
Score = 171 bits (432), Expect = 2e-40
Identities = 89/110 (80%), Positives = 93/110 (84%), Gaps = 1/110 (0%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GDIGY KELIKFKGFQVPPAELEALLISHP I+DAAVVPQKD AAGEV
Sbjct: 461 GDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDAAAGEV 520
Query: 183 PVAFVVPSS-GAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVVPS+ G ELTEEAVKEFISKQVVFYKRLHKVYF+H+IPKSPSGKI
Sbjct: 521 PVAFVVPSNDGFELTEEAVKEFISKQVVFYKRLHKVYFVHSIPKSPSGKI 570
>gb|AHL44980.1| 4-coumarate:coenzyme A ligase 4 [Fraxinus mandshurica]
Length = 573
Score = 170 bits (431), Expect = 3e-40
Identities = 86/109 (78%), Positives = 93/109 (85%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GDIGY KELIKFKGFQVPPAELEALL+SHP I+DAAVVPQKDEAAGEV
Sbjct: 446 GDIGYVDDDDDIFIVDRVKELIKFKGFQVPPAELEALLVSHPNIADAAVVPQKDEAAGEV 505
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVV S+G EL+EEAVKEFI+KQVVFYK+LHKVYFIHAIPKSP+GKI
Sbjct: 506 PVAFVVRSNGFELSEEAVKEFIAKQVVFYKKLHKVYFIHAIPKSPAGKI 554
>gb|EPS66025.1| hypothetical protein M569_08750, partial [Genlisea aurea]
Length = 208
Score = 170 bits (430), Expect = 4e-40
Identities = 88/110 (80%), Positives = 93/110 (84%), Gaps = 1/110 (0%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GD+GY KELIKFKGFQVPPAELEALLISHP I+DAAVVPQKDEAAGEV
Sbjct: 89 GDVGYVDDDDDIFIVDRVKELIKFKGFQVPPAELEALLISHPNIADAAVVPQKDEAAGEV 148
Query: 183 PVAFVVPSSG-AELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVV SSG EL+EEAVKEF+SKQVVFYKRLHKVYF+HAIPKSPSGKI
Sbjct: 149 PVAFVVRSSGLEELSEEAVKEFVSKQVVFYKRLHKVYFVHAIPKSPSGKI 198
>ref|XP_004511423.1| PREDICTED: 4-coumarate--CoA ligase 2-like isoform X1 [Cicer
arietinum]
Length = 590
Score = 168 bits (426), Expect = 1e-39
Identities = 85/109 (77%), Positives = 91/109 (83%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GD+GY KELIKFKGFQVPPAELE LL+SHP I+DAAVVPQKD AAGEV
Sbjct: 466 GDVGYIDDDDEIFIVDRVKELIKFKGFQVPPAELEGLLVSHPSIADAAVVPQKDVAAGEV 525
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVV S+G +LTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSP+GKI
Sbjct: 526 PVAFVVRSNGFDLTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPAGKI 574
>ref|XP_006443197.1| hypothetical protein CICLE_v10019532mg [Citrus clementina]
gi|568850424|ref|XP_006478914.1| PREDICTED:
4-coumarate--CoA ligase 2-like [Citrus sinensis]
gi|557545459|gb|ESR56437.1| hypothetical protein
CICLE_v10019532mg [Citrus clementina]
Length = 560
Score = 168 bits (425), Expect = 1e-39
Identities = 82/109 (75%), Positives = 91/109 (83%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GDIGY KE+IKFKGFQVPPAE+EALL+SHP I DAAVVPQKDE AGEV
Sbjct: 436 GDIGYVDDDDEVFIVDRVKEIIKFKGFQVPPAEIEALLLSHPSIGDAAVVPQKDEVAGEV 495
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVV S+G ELTEEA+KE+I+KQVVFYKRLHK+YF+HAIPKSPSGKI
Sbjct: 496 PVAFVVRSNGFELTEEAIKEYIAKQVVFYKRLHKIYFVHAIPKSPSGKI 544
>gb|AGW16013.1| 4-coumarate:coenzyme A ligase 1 [Pueraria montana var. lobata]
Length = 567
Score = 168 bits (425), Expect = 1e-39
Identities = 84/109 (77%), Positives = 91/109 (83%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GD+GY KELIKFKGFQVPPAELE LL+SHP I+DAAVVPQKD AAGEV
Sbjct: 442 GDVGYVDDDDEIFIVDRVKELIKFKGFQVPPAELEGLLVSHPSIADAAVVPQKDVAAGEV 501
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVV S+G +LTEEAVKEFI+KQVVFYKRLHKVYF+HAIPKSPSGKI
Sbjct: 502 PVAFVVRSNGFDLTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKI 550
>ref|XP_004307864.1| PREDICTED: LOW QUALITY PROTEIN: 4-coumarate--CoA ligase 2-like
[Fragaria vesca subsp. vesca]
Length = 586
Score = 168 bits (425), Expect = 1e-39
Identities = 84/109 (77%), Positives = 92/109 (84%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GD+GY KELIKFKGFQVPPAELE+LL+SH I+DAAVVPQKD+AAGEV
Sbjct: 462 GDVGYVDDDGEVFIVDRVKELIKFKGFQVPPAELESLLVSHQSIADAAVVPQKDDAAGEV 521
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVV S+G ELTEEAVKEFI+KQVVFYKRLHKVYF+HAIPKSPSGKI
Sbjct: 522 PVAFVVRSNGNELTEEAVKEFIAKQVVFYKRLHKVYFVHAIPKSPSGKI 570
>ref|XP_007219508.1| hypothetical protein PRUPE_ppa022401mg, partial [Prunus persica]
gi|462415970|gb|EMJ20707.1| hypothetical protein
PRUPE_ppa022401mg, partial [Prunus persica]
Length = 441
Score = 168 bits (425), Expect = 1e-39
Identities = 83/109 (76%), Positives = 93/109 (85%)
Frame = +3
Query: 3 GDIGYXXXXXXXXXXXXXKELIKFKGFQVPPAELEALLISHPQISDAAVVPQKDEAAGEV 182
GD+G+ KELIKFKGFQVPPAELE+LL++HP I+DAAVVPQKDEAAGEV
Sbjct: 317 GDVGFVDDDEEVFIVDRVKELIKFKGFQVPPAELESLLVNHPSIADAAVVPQKDEAAGEV 376
Query: 183 PVAFVVPSSGAELTEEAVKEFISKQVVFYKRLHKVYFIHAIPKSPSGKI 329
PVAFVV S+G +LTEEAVKEFI+KQVVFYKRLHKVYFIHAIPKSP+GKI
Sbjct: 377 PVAFVVRSNGLQLTEEAVKEFIAKQVVFYKRLHKVYFIHAIPKSPTGKI 425