BLASTX nr result
ID: Magnolia22_contig00017323
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Magnolia22_contig00017323
(1729 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BAF80448.1 corytuberine synthase [Coptis japonica var. dissecta] 536 0.0
AJD20229.1 corytuberine synthase [Sinopodophyllum hexandrum] 528 0.0
XP_010253990.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 500 e-170
XP_010253989.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 479 e-162
O64899.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 444 e-148
O64900.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 443 e-147
AKH61487.1 NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from... 443 e-147
Q9SP06.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase iso... 437 e-145
ACO90224.1 putative (S)-N-methylcoclaurine 3'-hydroxylase, parti... 437 e-145
AAF61400.1 (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papav... 435 e-144
P47195.1 RecName: Full=Berbamunine synthase; AltName: Full=(S)-N... 432 e-143
Q9FXW4.1 RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydrox... 426 e-141
XP_002513347.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 422 e-139
ABS19627.1 (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis] 421 e-139
XP_010662515.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 421 e-138
CAN62647.1 hypothetical protein VITISV_013219 [Vitis vinifera] 415 e-136
XP_002274562.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 415 e-136
XP_002283415.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 415 e-136
XP_002283461.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 414 e-135
XP_019072647.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hyd... 410 e-134
>BAF80448.1 corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 536 bits (1381), Expect = 0.0
Identities = 256/481 (53%), Positives = 347/481 (72%), Gaps = 6/481 (1%)
Frame = +3
Query: 27 IWLLVPLVLIF--LFKPSSNKNLPPGPRPWPIIGNMMYVLSGA---FHIELMKLARVHGP 191
++ L+P++L+F L KP KNLPPGP PWP+IGN+ + + HI L +AR HGP
Sbjct: 7 LFSLIPVILVFILLLKPKY-KNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMARTHGP 65
Query: 192 LMLLKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHIEHSLIWSDCTDEWK 371
+M+L G++P ++ ST E+AME+L+THDR FS R + S R+ HI++SL+WSDCTD WK
Sbjct: 66 IMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWK 125
Query: 372 NLRRIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILGHGI 551
LR+I +TE+FS KML + VR++KV+E++D++R EG + I+ VFGTLLNILG+ +
Sbjct: 126 LLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVV 185
Query: 552 FSKDVFQFGGKGNEFGMQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFDEVI 731
FSKDVF +G + ++ G+Q IR+M+ + E N+A+FYP F V+
Sbjct: 186 FSKDVFVYGDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDERFIRVM 245
Query: 732 FTWKDTIKERRANRDPSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVEWAMAE 911
W+ T+KER+ANR+ D+L+VLL NDF+DAQINA+ LE+FGPGSE+SS T+EW +AE
Sbjct: 246 KMWEGTVKERKANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVIAE 305
Query: 912 LLKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPHRTIET 1091
L+K P +MAK+R E+N VG + + E+ LP L YLQACIKE +RLHP PFL+P R ET
Sbjct: 306 LIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRLHPAAPFLLPRRAAET 365
Query: 1092 CQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGKH-QLIPFGVGR 1268
C+VMGYTIPK+ + +NAYAIGRDP +W DP TF PERFL++D+D+ G H Q IPFG GR
Sbjct: 366 CEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDFHGAHYQFIPFGSGR 425
Query: 1269 RICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVAVPKVR 1448
R C G+PLA RTIPL++GSL+H +D+ LP G + +DL M E +SLTL I+PSL VPK R
Sbjct: 426 RTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLAIDPSLCVVPKAR 485
Query: 1449 A 1451
A
Sbjct: 486 A 486
>AJD20229.1 corytuberine synthase [Sinopodophyllum hexandrum]
Length = 483
Score = 528 bits (1360), Expect = 0.0
Identities = 254/479 (53%), Positives = 349/479 (72%), Gaps = 3/479 (0%)
Frame = +3
Query: 21 ALIWLLVPLVLIFLFKPSSNKNLPPGPRPWPIIGNMMYVLSGAF--HIELMKLARVHGPL 194
AL++L+ ++L LFKP S KNLPPGP+PWPIIGN+ + S A+ H+ L +AR HGP+
Sbjct: 6 ALLYLIPTILLYILFKPRS-KNLPPGPKPWPIIGNIPALFSSAYPMHVALTNMARTHGPI 64
Query: 195 MLLKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHIEHSLIWSDCTDEWKN 374
M+L G++P + ST E+AME+L+THDR+FSGRAV S R+ HI++SL+W+DC++ WK
Sbjct: 65 MILWLGTQPTAVASTTEAAMEILKTHDRSFSGRAVRMSFRLKHHIKYSLVWADCSEYWKL 124
Query: 375 LRRIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILGHGIF 554
LR+I +TE+FS KML + VR++KV+E+V++++ EG + I+ +VFGTLLNILG+ IF
Sbjct: 125 LRKIVRTEIFSPKMLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNVIF 184
Query: 555 SKDVFQFGGKGNEFGMQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFDEVIF 734
SKDVF++ G + MQ I++M+ + NLA+F+PI F EV+
Sbjct: 185 SKDVFRYVGADGD-KMQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVMK 243
Query: 735 TWKDTIKERRANRDPSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVEWAMAEL 914
WK T++ER+ R+ D+L+VLL NDF+DAQINAV LE+FGPGSE+SS T EWA+AEL
Sbjct: 244 MWKGTVEERKVKRNEEPKDMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWALAEL 303
Query: 915 LKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPHRTIETC 1094
+K+P +AK+R E+++ VG + + E+ LP L YLQACIKE +RLHP PFL+PHR +ETC
Sbjct: 304 IKNPHILAKVRQELDQVVGKSTVKESDLPHLPYLQACIKETMRLHPAAPFLLPHRAVETC 363
Query: 1095 QVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGKH-QLIPFGVGRR 1271
VMGYTIPKD + +N YAIGRDP W +P TF PERFL++D+DY GKH + IPFG GRR
Sbjct: 364 PVMGYTIPKDTQVLVNIYAIGRDPKTWKEPTTFKPERFLESDVDYYGKHFEFIPFGAGRR 423
Query: 1272 ICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVAVPKVR 1448
C G+PLA RTIPL++ +L+ T DWSLP+G DL M E +SL+L ++P+L +PK R
Sbjct: 424 QCVGMPLATRTIPLIVSNLVQTLDWSLPDGKLPQDLVMNESLSLSLALDPTLTVIPKSR 482
>XP_010253990.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 500 bits (1287), Expect = e-170
Identities = 245/481 (50%), Positives = 338/481 (70%), Gaps = 3/481 (0%)
Frame = +3
Query: 15 IMALIWLLVPLVLIFLFKPSSNKNLPPGPRPWPIIGNMMYVLSGAFHIELMKLARVHGPL 194
+++ I+ L+ ++ + LF S NLPPGP WPIIG ++ L H+EL KLA+ GPL
Sbjct: 4 LVSFIFFLLSILSVVLFLKPSPNNLPPGPFSWPIIGTLLPKLKKQPHVELSKLAQRFGPL 63
Query: 195 MLLKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHIEHSLIWSDCTDEWKN 374
MLLKFG +PV++ S+ +A+EVL+ DR SGR PHSVRI G+IEHS++W+DCTD WK
Sbjct: 64 MLLKFGVEPVVVASSHVAAVEVLKNQDRLLSGRFAPHSVRIKGYIEHSMVWADCTDYWKM 123
Query: 375 LRRIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILGHGIF 554
+R++ +TELFSTKMLD+ A R+EKVSE++ ++ EG + V+FG++LNILG IF
Sbjct: 124 VRKVWRTELFSTKMLDIQAHAREEKVSELMKFLIRKEGEKVNFADVIFGSILNILGALIF 183
Query: 555 SKDVFQFGGK-GNEFGMQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFDEVI 731
SKDV+ F + N GM+ IR+++ LA NLAD YPI +C +
Sbjct: 184 SKDVYDFEDRTDNNLGMKGMIRQLMILAAIPNLADLYPILGGSDFQGLRKASAACVKRMN 243
Query: 732 FTWKDTIKERRANRDPSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVEWAMAE 911
+W +K+RR N D SK+D L+VLLD+ FSD QI+A+LLE+FGPGS++S++T+EWAMAE
Sbjct: 244 ESWAAIVKQRRKNDDHSKNDFLQVLLDSGFSDPQIDAMLLETFGPGSDTSTSTIEWAMAE 303
Query: 912 LLKHPDKMAKLRDEMNREV-GPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPHRTIE 1088
LL++P+K+ K+R+E++R + + E+ LP+L YL AC+KE LRLHP + FL+PHR +E
Sbjct: 304 LLRNPEKLVKVREELDRVIRRSNNVKESDLPNLPYLHACVKETLRLHPPVTFLLPHRAME 363
Query: 1089 TCQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGK-HQLIPFGVG 1265
TCQ+M YTIPK + +N YAIGRD W P +F PERFL+++LDY G Q IPFG G
Sbjct: 364 TCQMMNYTIPKGCQLMVNTYAIGRDSKTWEKPLSFLPERFLNSELDYQGNDFQYIPFGAG 423
Query: 1266 RRICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVAVPKV 1445
RRICPG+ LA R + L++ SL+HTFDWSLP+GM D+LDM +K L L + LV +PK+
Sbjct: 424 RRICPGLSLATRVVRLILASLLHTFDWSLPDGMHPDELDMNDKFGLALQKDIPLVVIPKL 483
Query: 1446 R 1448
R
Sbjct: 484 R 484
>XP_010253989.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 486
Score = 479 bits (1234), Expect = e-162
Identities = 234/479 (48%), Positives = 333/479 (69%), Gaps = 4/479 (0%)
Frame = +3
Query: 24 LIWLLVPLVLIFLFKPSSNKNLPPGPRPWPIIGNMMYVLSGAFHIELMKLARVHGPLMLL 203
+++LL L L+ KPSS K LPPGP WPIIG + + ++EL KLA+ +GPLML
Sbjct: 8 ILFLLSILSLVLFLKPSSKK-LPPGPFSWPIIGTQLPSPTMKPNLELFKLAQRYGPLMLF 66
Query: 204 KFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHIEHSLIWSDCTDEWKNLRR 383
+FG + V++ S +AMEVL+ DR SGR +SVR+ G+IE+S++W+DCTD WK +R+
Sbjct: 67 RFGFENVVVASNHVAAMEVLKNQDRVLSGRFKANSVRVKGYIEYSMVWADCTDYWKMVRK 126
Query: 384 IAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILGHGIFSKD 563
I +TELFSTKMLD+HA VR+EKVSE++ ++R EG + V+FG +LN+LG I+SKD
Sbjct: 127 ILRTELFSTKMLDVHAHVREEKVSELMKFLRRKEGEEVNFVDVIFGCILNMLGALIYSKD 186
Query: 564 VFQFGGKGN-EFGMQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVK-SCFDEVIFT 737
V+ F K + GM+ IR+++ LA N+AD YPI +C + +
Sbjct: 187 VYDFEDKTDINLGMKGMIRQLMILAATPNIADLYPIFFDGSDFQGLRKESAACVKRMSES 246
Query: 738 WKDTIKERRANRDPSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVEWAMAELL 917
W I ERR N D +K+D+L+VLLD+ FSD QI+A+ LE+FGPGS++S++T+EWA+AELL
Sbjct: 247 WAAIINERRKNNDHTKNDLLQVLLDSGFSDPQIDAIFLETFGPGSDTSASTIEWALAELL 306
Query: 918 KHPDKMAKLRDEMNREVG-PTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPHRTIETC 1094
++P+K+ KL +E++R +G + ++ LP+L YL AC+KE LRLHP +PFLIPH +E+C
Sbjct: 307 RNPEKLVKLHEELDRVIGRNNTVKDSDLPNLPYLHACVKETLRLHPPVPFLIPHIALESC 366
Query: 1095 QVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGKH-QLIPFGVGRR 1271
+VM YTIPK E +N YAIGRDP W +P++F PERFL++++DY G H Q IPFG GRR
Sbjct: 367 EVMNYTIPKGSEVLVNLYAIGRDPTTWDNPNSFLPERFLNSEVDYQGNHFQYIPFGAGRR 426
Query: 1272 ICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVAVPKVR 1448
+CPG+ L R + L++ +L+HTFDWSLP GM D+LDM ++ + LV +P +R
Sbjct: 427 MCPGMSLGTRVVRLILAALVHTFDWSLPGGMHQDELDMADRFGVGFQKETPLVVIPTLR 485
>O64899.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1 AAC39452.1
(S)-N-methylcoclaurine 3'-hydroxylase, partial
[Eschscholzia californica]
Length = 487
Score = 444 bits (1142), Expect = e-148
Identities = 214/483 (44%), Positives = 315/483 (65%), Gaps = 6/483 (1%)
Frame = +3
Query: 18 MALIWLLVPLVLIFLFKPSSNKNLPPGPRPWPIIGNMMYVLSGAFHIELMKLARVHGPLM 197
+ALI +++ +L LF S +KNLPPGP+PWPI+GN++ L H + +LA+ +G +
Sbjct: 5 VALIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQ-LGEKPHAQFAELAQTYGDIF 63
Query: 198 LLKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHIEHSLIWSDCTDEWKNL 377
LK G++ V++ ST +A E+L+THDR S R V S R+ GH+E+S++WSDCT+ WKNL
Sbjct: 64 TLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNL 123
Query: 378 RRIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILGHGIFS 557
R++ +TELF+ KM++ A VR++K EMV+Y+ + +G + I V+FGTL+NI G+ IFS
Sbjct: 124 RKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFS 183
Query: 558 KDVFQFGGKGNEFG-MQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFDEVIF 734
+++F+ G + ++ + +M+ L N AD++P+ V C +
Sbjct: 184 QNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYA 243
Query: 735 TWKDTIKERRANRD----PSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVEWA 902
W ++ER+ + SK+D ++V LD+ +D QINA+L+E FG G+E+S++T+EWA
Sbjct: 244 IWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWA 303
Query: 903 MAELLKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPHRT 1082
M EL K+P AK+R E+ VG ++ E+ P+L YL+A +KE LRLHP P L+P R
Sbjct: 304 MTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRA 363
Query: 1083 IETCQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGK-HQLIPFG 1259
+ETC ++ YTIPKD + +NA+ IGRDP WTDP TF PERFL++ +D+ G LIPFG
Sbjct: 364 LETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLIPFG 423
Query: 1260 VGRRICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVAVP 1439
GRRICPG+P+AN+ I LL+ + + DW LPNGM D L + EK LTL P L VP
Sbjct: 424 AGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVP 483
Query: 1440 KVR 1448
K R
Sbjct: 484 KSR 486
>O64900.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2 AAC39453.1
(S)-N-methylcoclaurine 3'-hydroxylase [Eschscholzia
californica]
Length = 488
Score = 443 bits (1140), Expect = e-147
Identities = 214/483 (44%), Positives = 314/483 (65%), Gaps = 6/483 (1%)
Frame = +3
Query: 18 MALIWLLVPLVLIFLFKPSSNKNLPPGPRPWPIIGNMMYVLSGAFHIELMKLARVHGPLM 197
+ALI +++ +L LF S +KNLPPGP+PWPI+GN++ L H + +LA+ +G +
Sbjct: 6 VALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQ-LGEKPHAQFAELAQTYGDIF 64
Query: 198 LLKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHIEHSLIWSDCTDEWKNL 377
LK G++ V++ ST +A E+L+THDR S R V S R+ GH+E+S++WSDCT+ WKNL
Sbjct: 65 TLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNL 124
Query: 378 RRIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILGHGIFS 557
R++ +TELF+ KM++ A VR++K EMV+Y+ + +G + I V+FGTL+NI G+ IFS
Sbjct: 125 RKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFS 184
Query: 558 KDVFQFGGKGNEFG-MQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFDEVIF 734
+++F+ G + ++ + +M+ L N AD++P+ V C +
Sbjct: 185 QNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYA 244
Query: 735 TWKDTIKERRANRD----PSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVEWA 902
W ++ER+ + SK+D ++V LD+ +D QINA+L+E FG G+E+S++T+EWA
Sbjct: 245 IWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWA 304
Query: 903 MAELLKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPHRT 1082
M EL K+P AKLR E+ VG ++ E+ P+L YL+A +KE LRLHP P L+P R
Sbjct: 305 MTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRA 364
Query: 1083 IETCQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGK-HQLIPFG 1259
+ETC ++ YTIPKD + +NA+ IGRDP W DP TF PERFL++ +D+ G LIPFG
Sbjct: 365 LETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIPFG 424
Query: 1260 VGRRICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVAVP 1439
GRRICPG+P+AN+ I LL+ + + DW LPNGM D L + EK LTL P L VP
Sbjct: 425 AGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVP 484
Query: 1440 KVR 1448
K R
Sbjct: 485 KSR 487
>AKH61487.1 NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 443 bits (1140), Expect = e-147
Identities = 214/483 (44%), Positives = 314/483 (65%), Gaps = 6/483 (1%)
Frame = +3
Query: 18 MALIWLLVPLVLIFLFKPSSNKNLPPGPRPWPIIGNMMYVLSGAFHIELMKLARVHGPLM 197
+ALI +++ +L LF S +KNLPPGP+PWPI+GN++ L H + +LA+ +G +
Sbjct: 6 VALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQ-LGEKPHAQFAELAQTYGDIF 64
Query: 198 LLKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHIEHSLIWSDCTDEWKNL 377
LK G++ V++ ST +A E+L+THDR S R V S R+ GH+E+S++WSDCT+ WKNL
Sbjct: 65 TLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNL 124
Query: 378 RRIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILGHGIFS 557
R++ +TELF+ KM++ A VR++K EMV+Y+ + +G + I V+FGTL+NI G+ IFS
Sbjct: 125 RKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFS 184
Query: 558 KDVFQFGGKGNEFG-MQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFDEVIF 734
+++F+ G + ++ + +M+ L N AD++P+ V C +
Sbjct: 185 QNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYA 244
Query: 735 TWKDTIKERRANRD----PSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVEWA 902
W ++ER+ + SK+D ++V LD+ +D QINA+L+E FG G+E+S++T+EWA
Sbjct: 245 IWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWA 304
Query: 903 MAELLKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPHRT 1082
M EL K+P AKLR E+ VG ++ E+ P+L YL+A +KE LRLHP P L+P R
Sbjct: 305 MTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRA 364
Query: 1083 IETCQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGK-HQLIPFG 1259
+ETC ++ YTIPKD + +NA+ IGRDP W DP TF PERFL++ +D+ G LIPFG
Sbjct: 365 LETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIPFG 424
Query: 1260 VGRRICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVAVP 1439
GRRICPG+P+AN+ I LL+ + + DW LPNGM D L + EK LTL P L VP
Sbjct: 425 AGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVP 484
Query: 1440 KVR 1448
K R
Sbjct: 485 KSR 487
>Q9SP06.1 RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1; AltName:
Full=Cytochrome P450 80B3 AAF05621.1
(S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver
somniferum]
Length = 481
Score = 437 bits (1124), Expect = e-145
Identities = 211/481 (43%), Positives = 310/481 (64%), Gaps = 5/481 (1%)
Frame = +3
Query: 21 ALIWLLVPLVLIFLFKPSSNKNLPPGPRPWPIIGNMMYVLSGAFHIELMKLARVHGPLML 200
+L+ +++ L +F+ SS K LPPGP+PWPI+GN++ L H + +LA +G L
Sbjct: 1 SLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQ-LGEKPHSQFAQLAETYGDLFS 59
Query: 201 LKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHIEHSLIWSDCTDEWKNLR 380
LK GS+ V++ ST +A E+L+THDR SGR V S R+ H+E+S++WS+C + WK LR
Sbjct: 60 LKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLR 119
Query: 381 RIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILGHGIFSK 560
++ +TELF+ KM++ A VR+ K EMV+Y+++N G + I VVFGTL+NI G+ IFS+
Sbjct: 120 KVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQ 179
Query: 561 DVFQFGGKGN-EFGMQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFDEVIFT 737
++F+ G + + M++ + +M+ L N AD++P V C +
Sbjct: 180 NIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSV 239
Query: 738 WKDTIKERRA---NRDPSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVEWAMA 908
W +KER+ + + K+D +E+LLD+ D QINA+L+E FG G+E+S++T+EWA++
Sbjct: 240 WGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALS 299
Query: 909 ELLKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPHRTIE 1088
EL K+P A +R E+ VG + E+ +P++ YLQA +KE LRLHP P L+P R +E
Sbjct: 300 ELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALE 359
Query: 1089 TCQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGK-HQLIPFGVG 1265
TC+V+ YTIPK+ + +NA+ IGRDP WTDP F PERFL++ +D+ G +LIPFG G
Sbjct: 360 TCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAG 419
Query: 1266 RRICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVAVPKV 1445
RRICPG+PLA + I L++ SL+ FDW P GM L M EK LTL P L VPK
Sbjct: 420 RRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKT 479
Query: 1446 R 1448
R
Sbjct: 480 R 480
>ACO90224.1 putative (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver
bracteatum]
Length = 486
Score = 437 bits (1124), Expect = e-145
Identities = 208/482 (43%), Positives = 313/482 (64%), Gaps = 5/482 (1%)
Frame = +3
Query: 18 MALIWLLVPLVLIFLFKPSSNKNLPPGPRPWPIIGNMMYVLSGAFHIELMKLARVHGPLM 197
++L+ +++ L +F+ SS K LPPGP+PWPI+GN++ L H + +LA+ +G L
Sbjct: 5 VSLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQ-LGEKPHSQFAELAKTYGDLF 63
Query: 198 LLKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHIEHSLIWSDCTDEWKNL 377
LK GS+ V++ ST +A E+L+THDR SGR V S R+ H+E+S++WS+C + WK L
Sbjct: 64 TLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKL 123
Query: 378 RRIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILGHGIFS 557
R++ + ELF+ KM++ A +R+ K EMV++++ N+G + I VVFGTL+NI G+ IFS
Sbjct: 124 RKVCRAELFTQKMIESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFS 183
Query: 558 KDVFQFGGKGN-EFGMQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFDEVIF 734
+++F+ G + + M++ + +M+ L N AD++P V C +
Sbjct: 184 QNIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYS 243
Query: 735 TWKDTIKERRA---NRDPSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVEWAM 905
W +KER+ + + K+D +E+LLD+ D QINA+L+E FG G+E+S++T+EWA+
Sbjct: 244 VWGAMLKERKIAKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWAL 303
Query: 906 AELLKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPHRTI 1085
+EL K+P+ A +R E+ VG + E+ +P++ YLQA +KE LRLHP P L+P R +
Sbjct: 304 SELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRAL 363
Query: 1086 ETCQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGK-HQLIPFGV 1262
ETC+V+ YTIPK+ + +NA+ IGRDP WTDP F PERFL++ +D+ G +LIPFG
Sbjct: 364 ETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFAPERFLNSSIDFKGNDFELIPFGA 423
Query: 1263 GRRICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVAVPK 1442
GRRICPG+PLA + I L++ +L+ FDW LP GM L M EK LTL P L VPK
Sbjct: 424 GRRICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIVPK 483
Query: 1443 VR 1448
R
Sbjct: 484 NR 485
>AAF61400.1 (S)-N-methylcoclaurine 3'-hydroxylase, partial [Papaver somniferum]
Length = 481
Score = 435 bits (1119), Expect = e-144
Identities = 210/481 (43%), Positives = 310/481 (64%), Gaps = 5/481 (1%)
Frame = +3
Query: 21 ALIWLLVPLVLIFLFKPSSNKNLPPGPRPWPIIGNMMYVLSGAFHIELMKLARVHGPLML 200
+L+ +++ L +F+ SS K LPPGP+PWPI+GN++ L H + +LA +G L
Sbjct: 1 SLVAVVITTFLYLIFRDSSPKGLPPGPKPWPIVGNLLQ-LGEKPHSQFAQLAETYGDLFS 59
Query: 201 LKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHIEHSLIWSDCTDEWKNLR 380
LK GS+ V++ ST +A E+L+THDR SGR V S R+ H+E+S++WS+C + WK LR
Sbjct: 60 LKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLR 119
Query: 381 RIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILGHGIFSK 560
++ +T+LF+ KM++ A VR+ K EMV+Y+++N G + I VVFGTL+NI G+ IFS+
Sbjct: 120 KVCRTDLFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQ 179
Query: 561 DVFQFGGKGN-EFGMQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFDEVIFT 737
++F+ G + + M++ + +M+ L N AD++P V C +
Sbjct: 180 NIFKLGDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSV 239
Query: 738 WKDTIKERRA---NRDPSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVEWAMA 908
W +KE + + + K+D +E+LLD+ D QINA+L+E FG G+E+S++T+EWA++
Sbjct: 240 WGAMLKESKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALS 299
Query: 909 ELLKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPHRTIE 1088
EL K+P A +R E+ VG + E+ +P++ YLQA +KE LRLHP P L+P R +E
Sbjct: 300 ELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALE 359
Query: 1089 TCQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGK-HQLIPFGVG 1265
TC+V+ YTIPK+ + +NA+ IGRDP WTDP F PERFL++ +D+ G +LIPFG G
Sbjct: 360 TCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAG 419
Query: 1266 RRICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVAVPKV 1445
RRICPG+PLA + I L++ SL+ FDW LP GM L M EK LTL P L VPK
Sbjct: 420 RRICPGVPLATQFISLIVSSLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKT 479
Query: 1446 R 1448
R
Sbjct: 480 R 480
>P47195.1 RecName: Full=Berbamunine synthase; AltName:
Full=(S)-N-methylcoclaurine oxidase [C-O
phenol-coupling]; AltName: Full=CYPLXXX; AltName:
Full=Cytochrome P450 80 AAC48987.1 cytochrome P-450 CYP80
[Berberis stolonifera]
Length = 487
Score = 432 bits (1112), Expect = e-143
Identities = 217/476 (45%), Positives = 312/476 (65%), Gaps = 5/476 (1%)
Frame = +3
Query: 36 LVPLVLIFLFKPSSNKNLPPGPRPWPIIGNMMYVLSG---AFHIELMKLARVHGPLMLLK 206
LV L+ LFKP + NLPP P WPI+G++ ++S F + +A+ +GPL+ LK
Sbjct: 12 LVALLYFLLFKPK-HTNLPPSPPAWPIVGHLPDLISKNSPPFLDYMSNIAQKYGPLIHLK 70
Query: 207 FGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHIEHSLIWSDCTDEWKNLRRI 386
FG I ST+E+AMEVL+T+D+ SGR RI HI++S++WSD WK R+I
Sbjct: 71 FGLHSSIFASTKEAAMEVLQTNDKVLSGRQPLPCFRIKPHIDYSILWSDSNSYWKKGRKI 130
Query: 387 AKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILGHGIFSKDV 566
TE+FS KML + R+ +V++I G + + +FG LN+LGH +FSKDV
Sbjct: 131 LHTEIFSQKMLQAQEKNRERVAGNLVNFIMTKVGDVVELRSWLFGCALNVLGHVVFSKDV 190
Query: 567 FQFGGKGNEFGMQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFDEVIFTWKD 746
F++ + +E GM K I M+ + ++A ++P+ + F +I W+
Sbjct: 191 FEYSDQSDEVGMDKLIHGMLMTGGDFDVASYFPVLARFDLHGLKRKMDEQFKLLIKIWEG 250
Query: 747 TIKERRANRDPSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVEWAMAELLKHP 926
+ RRANR+P D+L+VL+ NDF++ QINA+ +E+FGPGS+++S +EWA+A+L+K+P
Sbjct: 251 EVLARRANRNPEPKDMLDVLIANDFNEHQINAMFMETFGPGSDTNSNIIEWALAQLIKNP 310
Query: 927 DKMAKLRDEMNREVG-PTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPHRTIETCQVM 1103
DK+AKLR+E++R VG + + E+H L YLQAC+KE +RL+P + +IPHR +ETCQVM
Sbjct: 311 DKLAKLREELDRVVGRSSTVKESHFSELPYLQACVKETMRLYPPISIMIPHRCMETCQVM 370
Query: 1104 GYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGKH-QLIPFGVGRRICP 1280
GYTIPK + +NA+AIGRDP W DP F PERFLD+D++Y+GK Q IPFG GRRICP
Sbjct: 371 GYTIPKGMDVHVNAHAIGRDPKDWKDPLKFQPERFLDSDIEYNGKQFQFIPFGSGRRICP 430
Query: 1281 GIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVAVPKVR 1448
G PLA R IPL++ SL+H F W LP+G+ + LDM E +L+L + L +PKVR
Sbjct: 431 GRPLAVRIIPLVLASLVHAFGWELPDGVPNEKLDMEELFTLSLCMAKPLRVIPKVR 486
>Q9FXW4.1 RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2; AltName: Full=Cytochrome P450 80B2 BAB12433.1
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 426 bits (1095), Expect = e-141
Identities = 216/481 (44%), Positives = 307/481 (63%), Gaps = 3/481 (0%)
Frame = +3
Query: 18 MALIWLLVPLVLIFLFKPSSNKNLPPGPRPWPIIGNMMYVLSGAFHIELMKLARVHGPLM 197
+A++ L L F+ + S KNLPPGPRP PI+GN++ L H E KLA+ +G L
Sbjct: 6 IAIVSFSFLLFLFFILRDSRPKNLPPGPRPSPIVGNLLQ-LGDKPHAEFAKLAQKYGELF 64
Query: 198 LLKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHIEHSLIWSDCTDEWKNL 377
LK GS+ V++ S+ +A E+L+THD+ SGR V S R+ H+E+S++WS+C D WK L
Sbjct: 65 SLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLL 124
Query: 378 RRIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILGHGIFS 557
R++ +TELF+ KM++ + +R+ K EMV ++R EG + I VVFGTL+NI G+ IFS
Sbjct: 125 RKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIFS 184
Query: 558 KDVFQFGGK-GNEFGMQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFDEVIF 734
KDVF G +++ + K++ + N AD++PI V ++
Sbjct: 185 KDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIYD 244
Query: 735 TWKDTIKERRANR-DPSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVEWAMAE 911
W +KERR + SK+D ++VLL+ D +INA+L+E FG G+E+S++T+EWA+ E
Sbjct: 245 VWGVMLKERRGTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEWAITE 304
Query: 912 LLKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPHRTIET 1091
L K P ++K+R E+ VG + E+ LP L YLQA +KE LRLHP P L+P R +ET
Sbjct: 305 LTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALET 364
Query: 1092 CQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGK-HQLIPFGVGR 1268
C VM YTIPK+ + +NA+AIGRDP W DP F PERFL +D+DY G +LIPFG GR
Sbjct: 365 CTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGR 424
Query: 1269 RICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVAVPKVR 1448
RICPG+PLA++ L++ +L+ F+WSLP GM +L M EK LTL +P L+ V K R
Sbjct: 425 RICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKAR 484
Query: 1449 A 1451
A
Sbjct: 485 A 485
>XP_002513347.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Ricinus communis] EEF48750.1 (S)-N-methylcoclaurine
3'-hydroxylase isozyme, putative [Ricinus communis]
Length = 496
Score = 422 bits (1086), Expect = e-139
Identities = 211/482 (43%), Positives = 311/482 (64%), Gaps = 11/482 (2%)
Frame = +3
Query: 30 WLLVPLVLIFLFK-----PSSNKNLPPGPRPWPIIGNMMYVLSGAFHIELMKLARVHGPL 194
+LL+PL+LI + + S + LPPGPR WPI+GN++ V HI + A+VHGPL
Sbjct: 13 FLLLPLILIIVMQILSSISSKRRPLPPGPRQWPILGNILQVGKNP-HISMANYAKVHGPL 71
Query: 195 MLLKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHI--EHSLIWS-DCTDE 365
+ L+ G++ V++ S+ +A E+L+THDR SGR +P + ++ +L+W+ C+D+
Sbjct: 72 ISLRLGTRVVVVASSPTAAAEILKTHDRLLSGRYIPATTPYEDNVLDRIALVWNPSCSDQ 131
Query: 366 WKNLRRIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILGH 545
WK LR + ++ELFS K ++ A +R++K++EM+D++ +G + I VVF T N + +
Sbjct: 132 WKFLRAMCRSELFSAKAIESQATLREKKLTEMLDFLTSKQGQIVNIGEVVFTTAFNTISN 191
Query: 546 GIFSKDVFQFGGKGNEFGMQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFDE 725
+FSKD+ F +GN ++ I ++ LA N+ADFYP+ +K+C +
Sbjct: 192 LLFSKDLLSFEDQGNAGELKTLISTLMELATCPNIADFYPVLTKLDPQGIKRKMKNCLER 251
Query: 726 VIFTWKDTIKERRAN--RDPSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVEW 899
+ W IKERR +D K D L+V L N F D QIN +LLE F G+++++TTVEW
Sbjct: 252 MFGVWDIYIKERRERHVKDARKTDFLDVFLSNGFDDHQINWLLLELFSAGADTTTTTVEW 311
Query: 900 AMAELLKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPHR 1079
AMAELLK + K+R+E+ E+G + E+H+P L YL AC+KE LRLHP +PFLIP R
Sbjct: 312 AMAELLKEITVLEKVREELETEIGKDMIRESHIPQLKYLNACVKETLRLHPPVPFLIPRR 371
Query: 1080 TIETCQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGKH-QLIPF 1256
E C+VM YTIPK + +N +AIGRDP+AW DP ++ PERFLD++LD+ G + + +PF
Sbjct: 372 APEACEVMNYTIPKHSQIIVNVWAIGRDPSAWEDPLSYRPERFLDSNLDFKGHNFEFLPF 431
Query: 1257 GVGRRICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVAV 1436
G GRRICPG+P+ R +PL++ SL+H FDWSL NG LDM +K S+TL L+ V
Sbjct: 432 GSGRRICPGLPMGTRQLPLILASLVHCFDWSLQNGDDPAMLDMNDKFSITLEKEQHLLVV 491
Query: 1437 PK 1442
PK
Sbjct: 492 PK 493
>ABS19627.1 (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 421 bits (1083), Expect = e-139
Identities = 214/480 (44%), Positives = 304/480 (63%), Gaps = 3/480 (0%)
Frame = +3
Query: 18 MALIWLLVPLVLIFLFKPSSNKNLPPGPRPWPIIGNMMYVLSGAFHIELMKLARVHGPLM 197
+A++ L L F+ + KNLPPGPRP PI+GN++ L E KLA+ +G L
Sbjct: 6 IAIVSFSFLLFLFFILRDPRQKNLPPGPRPLPIVGNLLQ-LGDKPRAEFAKLAQKYGQLF 64
Query: 198 LLKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHIEHSLIWSDCTDEWKNL 377
LK GS+ V++ S+ +A EVL+THD+ SGR V S R+ H+E+S++WS+C D WK L
Sbjct: 65 SLKLGSQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLL 124
Query: 378 RRIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILGHGIFS 557
R++ +TELF+ KM++ + +R+ K EMV ++R EG + I VVFGTL+NI G+ IFS
Sbjct: 125 RKVCRTELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLIFS 184
Query: 558 KDVFQFGGK-GNEFGMQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFDEVIF 734
KDVF G +++ + K++ + N AD++PI V ++
Sbjct: 185 KDVFDLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQIYD 244
Query: 735 TWKDTIKERRANR-DPSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVEWAMAE 911
W +KERR + S +D ++VLL+ D +INA+L+E FG G+E+S++T+EWA+ E
Sbjct: 245 VWGINLKERRGMKGSESNNDFVDVLLNAGLEDQKINALLMELFGTGTETSASTIEWAITE 304
Query: 912 LLKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPHRTIET 1091
L K P ++K+R E+ VG + E+ LP L YLQA +KE LRLHP P L+P R +ET
Sbjct: 305 LTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALET 364
Query: 1092 CQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGK-HQLIPFGVGR 1268
C VM YTIPK+ + +NA+AIGRDP W DP F PERFL +D+DY G +LIPFG GR
Sbjct: 365 CTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGR 424
Query: 1269 RICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVAVPKVR 1448
RICPG+PLA++ I L++ +L+ F+WSLP GM +L M EK LTL +P L+ V K R
Sbjct: 425 RICPGLPLASQFISLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKAR 484
>XP_010662515.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 515
Score = 421 bits (1082), Expect = e-138
Identities = 215/487 (44%), Positives = 312/487 (64%), Gaps = 8/487 (1%)
Frame = +3
Query: 6 SSRIMALIWLLVPLVLIFLF-KPSSNKNLPPGPRPWPIIGNMMYVLSGAFHIELMKLARV 182
SS + AL+ L L+L +F KP LPPGP PWPIIGN++ + H++L LA++
Sbjct: 13 SSFVYALLLLPFLLILKHIFLKPPP---LPPGPYPWPIIGNLLQLGKNP-HVKLASLAKL 68
Query: 183 HGPLMLLKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRI----VGHIEHSLIWS 350
HGPLM L+ G++ +++ S+ +A+EVL+THDR SGR V S+ + + H+ +
Sbjct: 69 HGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFA-K 127
Query: 351 DCTDEWKNLRRIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLL 530
+CT+ WKNLR I +TE+FS K ++ H +R+ KV E+V+++ EG + + +VF T+
Sbjct: 128 ECTNNWKNLRTICRTEMFSGKAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTIC 187
Query: 531 NILGHGIFSKDVFQFGGKGNEFGMQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVK 710
NIL + FS D+ F +G ++ I + L NL+DFYPI K
Sbjct: 188 NILSNTFFSMDLCDFEREG----LKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSK 243
Query: 711 SCFDEVIFTWKDTIKERRANRDP--SKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSS 884
++ TW+ T+KERR ++P S D+LE L+ F D QIN V+LE F G+++S+
Sbjct: 244 EALGRILATWEGTLKERRKQKNPGSSHRDLLEAFLEIRFEDDQINQVILELFSAGADTST 303
Query: 885 TTVEWAMAELLKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPF 1064
T+EWA+ +L+++PD M KLRDE+ + +G + + E+HLP L YLQAC+KE LRLHP P
Sbjct: 304 LTIEWAITQLIRNPDVMYKLRDELTKIIGESPVRESHLPHLPYLQACVKETLRLHPPAPL 363
Query: 1065 LIPHRTIETCQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGK-H 1241
L+PHR +ETCQVMGYTIPKD + F+N +A+GRDP W DP +F PERFLD+ L++ G
Sbjct: 364 LLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWDDPLSFTPERFLDSKLEFKGNDF 423
Query: 1242 QLIPFGVGRRICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINP 1421
+ IPFG GRRICPG+ L R +PL++ +L+H FDWSLP+ M + +DM E + +TL
Sbjct: 424 EYIPFGAGRRICPGMALGARQVPLVLATLVHLFDWSLPDNMDSAQIDMEEWLVITLRKEN 483
Query: 1422 SLVAVPK 1442
L VPK
Sbjct: 484 PLRLVPK 490
>CAN62647.1 hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 415 bits (1066), Expect = e-136
Identities = 222/484 (45%), Positives = 301/484 (62%), Gaps = 7/484 (1%)
Frame = +3
Query: 18 MALIWLLVPLVLIFLFKPSSNKNLPPGPRPWPIIGNMMYVLSGAFHIELMKLARVHGPLM 197
+ L+ LV L IFL P LPPGP PWPIIGN++ + H +L LA++HGPLM
Sbjct: 19 LLLLPFLVILKHIFLKPPP----LPPGPYPWPIIGNLLQMGKNP-HAKLANLAKLHGPLM 73
Query: 198 LLKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVG-HIEH-SLIWS-DCTDEW 368
L+ G++ +++ S+ +AMEVL+THDRA SGR + SV + + H S++++ DC W
Sbjct: 74 SLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSXSVPVKNPKLNHLSIVFAKDCNTNW 133
Query: 369 KNLRRIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILGHG 548
KNLR I + ELFS K ++ +R+ KV+E+V+++ EG + + +VF T+ NIL +
Sbjct: 134 KNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNK 193
Query: 549 IFSKDVFQFGGKGNEFGMQK-AIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFDE 725
FS D+ F +G G K I K NL+D+YPI K F+
Sbjct: 194 FFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFER 253
Query: 726 VIFTWKDTIKERRANRD--PSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVEW 899
+ TW+D +KERR R S D LE LL+ F D QIN V+LE F G+E+SS TVEW
Sbjct: 254 IPTTWEDILKERRTQRSNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVEW 313
Query: 900 AMAELLKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPHR 1079
AMAEL+++ D M KLR E+ + VG + + E+HLP L YLQAC+KE LRLHP P L+PH
Sbjct: 314 AMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRLHPPAPLLLPHL 373
Query: 1080 TIETCQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGK-HQLIPF 1256
ETCQVMGYTIPKD + F+N +A+ RDP W DP +F PERFLD+ LD+ G + IPF
Sbjct: 374 AAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPERFLDSKLDFKGNDFEYIPF 433
Query: 1257 GVGRRICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVAV 1436
G GRRICPG+ L R +PL++ + +H F WSLP M + LDM E + +TL L V
Sbjct: 434 GAGRRICPGLALGGRQVPLILATFVHLFGWSLPGNMDSAQLDMEEWLVITLRKEQPLRLV 493
Query: 1437 PKVR 1448
P+VR
Sbjct: 494 PRVR 497
>XP_002274562.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 415 bits (1066), Expect = e-136
Identities = 222/484 (45%), Positives = 301/484 (62%), Gaps = 7/484 (1%)
Frame = +3
Query: 18 MALIWLLVPLVLIFLFKPSSNKNLPPGPRPWPIIGNMMYVLSGAFHIELMKLARVHGPLM 197
+ L+ LV L IFL P LPPGP PWPIIGN++ + H +L LA++HGPLM
Sbjct: 19 LLLLPFLVILKHIFLKPPP----LPPGPYPWPIIGNLLQMGKNP-HAKLANLAKLHGPLM 73
Query: 198 LLKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVG-HIEH-SLIWS-DCTDEW 368
L+ G++ +++ S+ +AMEVL+THDRA SGR + SV + + H S++++ DC W
Sbjct: 74 SLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNW 133
Query: 369 KNLRRIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILGHG 548
KNLR I + ELFS K ++ +R+ KV+E+V+++ EG + + +VF T+ NIL +
Sbjct: 134 KNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNK 193
Query: 549 IFSKDVFQFGGKGNEFGMQK-AIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFDE 725
FS D+ F +G G K I K NL+D+YPI K F+
Sbjct: 194 FFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFER 253
Query: 726 VIFTWKDTIKERRANRD--PSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVEW 899
+ TW+D +KERR R S D LE LL+ F D QIN V+LE F G+E+SS TVEW
Sbjct: 254 IPTTWEDILKERRTQRSNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVEW 313
Query: 900 AMAELLKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPHR 1079
AMAEL+++ D M KLR E+ + VG + + E+HLP L YLQAC+KE LRLHP P L+PH
Sbjct: 314 AMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRLHPPAPLLLPHL 373
Query: 1080 TIETCQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGK-HQLIPF 1256
ETCQVMGYTIPKD + F+N +A+ RDP W DP +F PERFLD+ LD+ G + IPF
Sbjct: 374 AAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPERFLDSKLDFKGNDFEYIPF 433
Query: 1257 GVGRRICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVAV 1436
G GRRICPG+ L R +PL++ + +H F WSLP M + LDM E + +TL L V
Sbjct: 434 GAGRRICPGLALGGRQVPLILATFVHLFGWSLPGNMDSAQLDMEEWLVITLRKEQPLRLV 493
Query: 1437 PKVR 1448
P+VR
Sbjct: 494 PRVR 497
>XP_002283415.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 540
Score = 415 bits (1067), Expect = e-136
Identities = 217/485 (44%), Positives = 306/485 (63%), Gaps = 8/485 (1%)
Frame = +3
Query: 18 MALIWLLVPLVLIFLFKPSSNKN--LPPGPRPWPIIGNMMYVLSGAFHIELMKLARVHGP 191
+ L+ LLV L+L L SS K+ LPPGP PWPI+GN+++ L HI L + ++ +GP
Sbjct: 56 LLLLPLLVFLILKLLKDSSSLKSPPLPPGPSPWPILGNLLH-LGNMPHISLARFSQSYGP 114
Query: 192 LMLLKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHIEH--SLIWS-DCTD 362
L+ L+ GS+ +++ ST +AME+L+THDR SGR VPH+V + SL W+ +C
Sbjct: 115 LISLRLGSQILVVASTSSAAMEILKTHDRVLSGRYVPHAVPAKNSEINPMSLGWAVECNG 174
Query: 363 EWKNLRRIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILG 542
WKNLR + + ELFSTK+++ A V ++KV EMV ++ EG + + VVF T+LN L
Sbjct: 175 AWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLT 234
Query: 543 HGIFSKDVFQFGGKGNEFGMQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFD 722
+ S+D F + GM+ +RKM+ NL DFY I K
Sbjct: 235 TVLMSRDFISFEDDNKDGGMKGLVRKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIA 294
Query: 723 EVIFTWKDTIKERR--ANRDPSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVE 896
+ W+ I+ERR A+ DPSK D L +L+ + +SD QIN + +E G+++SS+T+E
Sbjct: 295 RICSMWESVIRERREGASDDPSKQDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLE 354
Query: 897 WAMAELLKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPH 1076
WAMAEL+K P+ M K+ +E+ RE+ + LP L YLQAC+KE LRLHP P L+P
Sbjct: 355 WAMAELIKSPESMKKVHEELAREISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPR 414
Query: 1077 RTIETCQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGKH-QLIP 1253
R +C+VM YTIPKD + ++NA+AIGRDP W DP F PERFL++ +D+ G + + IP
Sbjct: 415 RASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIP 474
Query: 1254 FGVGRRICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVA 1433
FG GRRICPG+P+A R +PL++ SL H FDWSLPNG D+LDM +K +TL L+
Sbjct: 475 FGAGRRICPGLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGVTLQKEQPLLI 534
Query: 1434 VPKVR 1448
+PKVR
Sbjct: 535 IPKVR 539
>XP_002283461.2 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
isoform X2 [Vitis vinifera]
Length = 540
Score = 414 bits (1065), Expect = e-135
Identities = 217/485 (44%), Positives = 306/485 (63%), Gaps = 8/485 (1%)
Frame = +3
Query: 18 MALIWLLVPLVLIFLFKPSSNKN--LPPGPRPWPIIGNMMYVLSGAFHIELMKLARVHGP 191
+ L+ LLV L+L L SS K+ LPPGP PWPI+GN+++ L HI L + ++ +GP
Sbjct: 56 LLLLPLLVFLILKLLKDSSSLKSPPLPPGPSPWPILGNLLH-LGNMPHISLARFSQSYGP 114
Query: 192 LMLLKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHIEH--SLIWS-DCTD 362
L+ L+ GS+ +++ ST +AME+L+THDR SGR VPH+V + SL W+ +C D
Sbjct: 115 LISLRLGSQILVVASTSSAAMEILKTHDRVLSGRYVPHAVPAKNSEINRMSLGWAVECND 174
Query: 363 EWKNLRRIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILG 542
WKNLR + + ELFSTK+++ A V ++KV EMV ++ EG + + VVF T+LN L
Sbjct: 175 AWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLT 234
Query: 543 HGIFSKDVFQFGGKGNEFGMQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFD 722
+ S+D F + GM+ +RKM+ NL DFY I K
Sbjct: 235 TVLMSRDFISFEDDYKDGGMKGLVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIA 294
Query: 723 EVIFTWKDTIKERR--ANRDPSKHDVLEVLLDNDFSDAQINAVLLESFGPGSESSSTTVE 896
+ W+ I+ERR A+ DPSK D L +L+ + +SD QIN + +E G+++SS+T+E
Sbjct: 295 RICSMWESVIRERREGASDDPSKQDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLE 354
Query: 897 WAMAELLKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIPH 1076
WAMAEL+K P+ M K+ +E+ RE+ + LP L YLQAC+KE LRLHP P L+P
Sbjct: 355 WAMAELIKSPESMKKVHEELAREISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPR 414
Query: 1077 RTIETCQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGKH-QLIP 1253
R +C+VM YTIPKD + ++NA+AIGRDP W DP F PERFL++ +D+ G + + IP
Sbjct: 415 RASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIP 474
Query: 1254 FGVGRRICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLVA 1433
FG GRRICPG+P+A R +PL++ SL H FDWSLPNG D+LDM +K + L L+
Sbjct: 475 FGAGRRICPGLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGIALQKEQPLLI 534
Query: 1434 VPKVR 1448
+PKVR
Sbjct: 535 IPKVR 539
>XP_019072647.1 PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
isoform X1 [Vitis vinifera]
Length = 541
Score = 410 bits (1053), Expect = e-134
Identities = 217/486 (44%), Positives = 306/486 (62%), Gaps = 9/486 (1%)
Frame = +3
Query: 18 MALIWLLVPLVLIFLFKPSSNKN--LPPGPRPWPIIGNMMYVLSGAFHIELMKLARVHGP 191
+ L+ LLV L+L L SS K+ LPPGP PWPI+GN+++ L HI L + ++ +GP
Sbjct: 56 LLLLPLLVFLILKLLKDSSSLKSPPLPPGPSPWPILGNLLH-LGNMPHISLARFSQSYGP 114
Query: 192 LMLLKFGSKPVIIVSTQESAMEVLRTHDRAFSGRAVPHSVRIVGHIEH--SLIWS-DCTD 362
L+ L+ GS+ +++ ST +AME+L+THDR SGR VPH+V + SL W+ +C D
Sbjct: 115 LISLRLGSQILVVASTSSAAMEILKTHDRVLSGRYVPHAVPAKNSEINRMSLGWAVECND 174
Query: 363 EWKNLRRIAKTELFSTKMLDMHARVRQEKVSEMVDYIRENEGGPIMITGVVFGTLLNILG 542
WKNLR + + ELFSTK+++ A V ++KV EMV ++ EG + + VVF T+LN L
Sbjct: 175 AWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLT 234
Query: 543 HGIFSKDVFQFGGKGNEFGMQKAIRKMIGLAVENNLADFYPIXXXXXXXXXXXXVKSCFD 722
+ S+D F + GM+ +RKM+ NL DFY I K
Sbjct: 235 TVLMSRDFISFEDDYKDGGMKGLVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIA 294
Query: 723 EVIFTWKDTIKERR--ANRDPSKHDVLEVLLDNDFSDAQINAVLL-ESFGPGSESSSTTV 893
+ W+ I+ERR A+ DPSK D L +L+ + +SD QIN + + E G+++SS+T+
Sbjct: 295 RICSMWESVIRERREGASDDPSKQDFLNILIRSGYSDDQINQLFMQELLTAGADTSSSTL 354
Query: 894 EWAMAELLKHPDKMAKLRDEMNREVGPTALTEAHLPSLHYLQACIKEILRLHPVLPFLIP 1073
EWAMAEL+K P+ M K+ +E+ RE+ + LP L YLQAC+KE LRLHP P L+P
Sbjct: 355 EWAMAELIKSPESMKKVHEELAREISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLP 414
Query: 1074 HRTIETCQVMGYTIPKDYETFINAYAIGRDPNAWTDPDTFCPERFLDTDLDYSGKH-QLI 1250
R +C+VM YTIPKD + ++NA+AIGRDP W DP F PERFL++ +D+ G + + I
Sbjct: 415 RRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFI 474
Query: 1251 PFGVGRRICPGIPLANRTIPLLIGSLIHTFDWSLPNGMKADDLDMTEKMSLTLVINPSLV 1430
PFG GRRICPG+P+A R +PL++ SL H FDWSLPNG D+LDM +K + L L+
Sbjct: 475 PFGAGRRICPGLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGIALQKEQPLL 534
Query: 1431 AVPKVR 1448
+PKVR
Sbjct: 535 IIPKVR 540