BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Glycyrrhiza34_contig00011232
         (500 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

KYP45666.1 Two-component response regulator ARR8 [Cajanus cajan]      173   7e-52
XP_007141019.1 hypothetical protein PHAVU_008G1605000g, partial ...   167   9e-51
XP_003543102.1 PREDICTED: two-component response regulator ORR9-...   168   8e-50
XP_019455274.1 PREDICTED: two-component response regulator ARR17...   166   5e-49
KRH18706.1 hypothetical protein GLYMA_13G077500 [Glycine max]         163   8e-48
XP_019416272.1 PREDICTED: two-component response regulator ORR9-...   153   3e-44
XP_019430526.1 PREDICTED: two-component response regulator ORR9-...   154   4e-44
XP_019416271.1 PREDICTED: two-component response regulator ORR9-...   153   6e-44
KYP56931.1 Two-component response regulator ARR9 [Cajanus cajan]      152   3e-43
XP_003523433.1 PREDICTED: two-component response regulator ORR9-...   151   5e-43
ACU23880.1 unknown [Glycine max]                                      151   5e-43
AFK44001.1 unknown [Medicago truncatula]                              151   7e-43
XP_003526642.1 PREDICTED: two-component response regulator ORR9-...   150   1e-42
XP_014500206.1 PREDICTED: two-component response regulator ORR9-...   150   1e-42
XP_017422043.1 PREDICTED: two-component response regulator ORR9-...   150   1e-42
XP_007137437.1 hypothetical protein PHAVU_009G127000g [Phaseolus...   150   2e-42
XP_019415488.1 PREDICTED: two-component response regulator ORR9-...   150   2e-42
XP_012078259.1 PREDICTED: two-component response regulator ARR8-...   149   2e-42
XP_016163494.1 PREDICTED: two-component response regulator ORR9-...   149   2e-42
OAY58284.1 hypothetical protein MANES_02G164700 [Manihot esculenta]   149   3e-42

>KYP45666.1 Two-component response regulator ARR8 [Cajanus cajan]
          Length = 204

 Score =  173 bits (438), Expect = 7e-52
 Identities = 95/135 (70%), Positives = 105/135 (77%), Gaps = 2/135 (1%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKR--SSWKDVPVMIMSSENVPSRISMCLEGGAEEF 327
           GIKVNLIMTDYCMPGMSGYDLLKRIK   SSWKDVPV+IMSSENVPSRI+MCLEGGAEEF
Sbjct: 77  GIKVNLIMTDYCMPGMSGYDLLKRIKLQGSSWKDVPVVIMSSENVPSRITMCLEGGAEEF 136

Query: 326 LLKPLQLSDLKKLQPYFLKSFDEQEXXXXXXXXXXXXXXXXXXSKRNAMSHKRGFLKLQF 147
           LLKPLQ SDLKKLQP FLKSF++Q                   ++R A+S + GF KLQF
Sbjct: 137 LLKPLQQSDLKKLQPCFLKSFEQQS------ADRSVASHNDNDNERTALSPEHGF-KLQF 189

Query: 146 VSAIATTMLQFWRSL 102
           VS IATT+L+FW SL
Sbjct: 190 VSTIATTILRFWNSL 204


>XP_007141019.1 hypothetical protein PHAVU_008G1605000g, partial [Phaseolus
           vulgaris] ESW13013.1 hypothetical protein
           PHAVU_008G1605000g, partial [Phaseolus vulgaris]
          Length = 129

 Score =  167 bits (424), Expect = 9e-51
 Identities = 91/133 (68%), Positives = 101/133 (75%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           G+KVN+IMTDYCMPGMSGYDLLKRIK SSWKDVPV+IMSSENVPSRI+MCLEGGAEEFLL
Sbjct: 1   GMKVNMIMTDYCMPGMSGYDLLKRIKGSSWKDVPVVIMSSENVPSRITMCLEGGAEEFLL 60

Query: 320 KPLQLSDLKKLQPYFLKSFDEQEXXXXXXXXXXXXXXXXXXSKRNAMSHKRGFLKLQFVS 141
           KPLQ SDLKKLQP FLKSFD                     +KRN +  + GF KLQF++
Sbjct: 61  KPLQPSDLKKLQPCFLKSFDNSS--EEDICAERSLSSRNDINKRN-LYPQHGF-KLQFLT 116

Query: 140 AIATTMLQFWRSL 102
            I TTML+FW SL
Sbjct: 117 TIVTTMLRFWNSL 129


>XP_003543102.1 PREDICTED: two-component response regulator ORR9-like [Glycine max]
           KHN33836.1 Two-component response regulator ARR8
           [Glycine soja] KRH18705.1 hypothetical protein
           GLYMA_13G077500 [Glycine max]
          Length = 211

 Score =  168 bits (425), Expect = 8e-50
 Identities = 93/136 (68%), Positives = 103/136 (75%), Gaps = 3/136 (2%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           GIKVN+IMTDYCMPGMSGYDLLKRIK SSWKDVPV+IMSSENVPSRISMCLEGGAE+FLL
Sbjct: 79  GIKVNMIMTDYCMPGMSGYDLLKRIKGSSWKDVPVVIMSSENVPSRISMCLEGGAEKFLL 138

Query: 320 KPLQLSDLKKLQPYFLKSFD---EQEXXXXXXXXXXXXXXXXXXSKRNAMSHKRGFLKLQ 150
           KPLQ SDL+KLQP FLKS D   EQ+                   K  A+  + GF KLQ
Sbjct: 139 KPLQQSDLEKLQPCFLKSSDNSSEQDQSAERSVASHDNDNYIDTIK--ALCPEHGF-KLQ 195

Query: 149 FVSAIATTMLQFWRSL 102
           FVS IATT+L+FW+SL
Sbjct: 196 FVSTIATTILRFWKSL 211


>XP_019455274.1 PREDICTED: two-component response regulator ARR17-like [Lupinus
           angustifolius] OIW04503.1 hypothetical protein
           TanjilG_13885 [Lupinus angustifolius]
          Length = 201

 Score =  166 bits (419), Expect = 5e-49
 Identities = 87/138 (63%), Positives = 99/138 (71%), Gaps = 5/138 (3%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           GIKVNLIMTDY MPGMSGYDLLKRIK S+W+DVPV+IMSSEN+PSRISMCLEGGAEEFL 
Sbjct: 66  GIKVNLIMTDYSMPGMSGYDLLKRIKESTWRDVPVVIMSSENIPSRISMCLEGGAEEFLS 125

Query: 320 KPLQLSDLKKLQPYFLKSFDEQEXXXXXXXXXXXXXXXXXXSKRNAMSHKRGF-----LK 156
           KPLQLSDLK+LQPYF+KSFD                      K N +S  +G       K
Sbjct: 126 KPLQLSDLKRLQPYFMKSFDNSS--QEDYANSSITSNNDNIIKNNGISKMKGMPVEHGFK 183

Query: 155 LQFVSAIATTMLQFWRSL 102
           L ++ AIAT +LQFW+SL
Sbjct: 184 LWYIIAIATAILQFWKSL 201


>KRH18706.1 hypothetical protein GLYMA_13G077500 [Glycine max]
          Length = 213

 Score =  163 bits (412), Expect = 8e-48
 Identities = 93/138 (67%), Positives = 103/138 (74%), Gaps = 5/138 (3%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKR--SSWKDVPVMIMSSENVPSRISMCLEGGAEEF 327
           GIKVN+IMTDYCMPGMSGYDLLKRIK   SSWKDVPV+IMSSENVPSRISMCLEGGAE+F
Sbjct: 79  GIKVNMIMTDYCMPGMSGYDLLKRIKLQGSSWKDVPVVIMSSENVPSRISMCLEGGAEKF 138

Query: 326 LLKPLQLSDLKKLQPYFLKSFD---EQEXXXXXXXXXXXXXXXXXXSKRNAMSHKRGFLK 156
           LLKPLQ SDL+KLQP FLKS D   EQ+                   K  A+  + GF K
Sbjct: 139 LLKPLQQSDLEKLQPCFLKSSDNSSEQDQSAERSVASHDNDNYIDTIK--ALCPEHGF-K 195

Query: 155 LQFVSAIATTMLQFWRSL 102
           LQFVS IATT+L+FW+SL
Sbjct: 196 LQFVSTIATTILRFWKSL 213


>XP_019416272.1 PREDICTED: two-component response regulator ORR9-like isoform X2
           [Lupinus angustifolius]
          Length = 199

 Score =  153 bits (387), Expect = 3e-44
 Identities = 75/86 (87%), Positives = 80/86 (93%), Gaps = 3/86 (3%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           G KVNLIMTDYCMPGMSGYDLLKR+K SSWKDVPV++MSSEN+PSRISMCLE GAEEFLL
Sbjct: 82  GTKVNLIMTDYCMPGMSGYDLLKRVKESSWKDVPVVVMSSENIPSRISMCLEEGAEEFLL 141

Query: 320 KPLQLSDLKKLQPYFLKSFD---EQE 252
           KPL+LSDLKKLQPYFLKSFD   EQE
Sbjct: 142 KPLKLSDLKKLQPYFLKSFDNSYEQE 167


>XP_019430526.1 PREDICTED: two-component response regulator ORR9-like isoform X1
           [Lupinus angustifolius] XP_019430533.1 PREDICTED:
           two-component response regulator ORR9-like isoform X1
           [Lupinus angustifolius]
          Length = 214

 Score =  154 bits (388), Expect = 4e-44
 Identities = 84/139 (60%), Positives = 96/139 (69%), Gaps = 6/139 (4%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           GIKVNLIMTDYCMPGM+GYDLLKRIK SSWKDVPV+IMSSENVPSRIS CLEGGA+EFLL
Sbjct: 79  GIKVNLIMTDYCMPGMNGYDLLKRIKGSSWKDVPVVIMSSENVPSRISRCLEGGAKEFLL 138

Query: 320 KPLQLSDLKKLQPYFLKSFDE-QEXXXXXXXXXXXXXXXXXXSKRNAMSHKRGF-----L 159
           KPLQ SDL+KLQPYF+ SFD                       K N M+  +G       
Sbjct: 139 KPLQFSDLEKLQPYFMTSFDNCSHQKYANSSKASNNGNNNNNIKNNDMTKLKGMHGEHSF 198

Query: 158 KLQFVSAIATTMLQFWRSL 102
           KL +V+A    +LQFW+S+
Sbjct: 199 KLWYVTA---AILQFWKSI 214


>XP_019416271.1 PREDICTED: two-component response regulator ORR9-like isoform X1
           [Lupinus angustifolius]
          Length = 219

 Score =  153 bits (387), Expect = 6e-44
 Identities = 75/86 (87%), Positives = 80/86 (93%), Gaps = 3/86 (3%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           G KVNLIMTDYCMPGMSGYDLLKR+K SSWKDVPV++MSSEN+PSRISMCLE GAEEFLL
Sbjct: 82  GTKVNLIMTDYCMPGMSGYDLLKRVKESSWKDVPVVVMSSENIPSRISMCLEEGAEEFLL 141

Query: 320 KPLQLSDLKKLQPYFLKSFD---EQE 252
           KPL+LSDLKKLQPYFLKSFD   EQE
Sbjct: 142 KPLKLSDLKKLQPYFLKSFDNSYEQE 167


>KYP56931.1 Two-component response regulator ARR9 [Cajanus cajan]
          Length = 228

 Score =  152 bits (383), Expect = 3e-43
 Identities = 74/80 (92%), Positives = 76/80 (95%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           GIKVNLIMTDYCMPGMSGYDLLKR+K SSWKDVPV+IMSSENVPSRISMCLE GAEEFLL
Sbjct: 93  GIKVNLIMTDYCMPGMSGYDLLKRVKGSSWKDVPVVIMSSENVPSRISMCLEEGAEEFLL 152

Query: 320 KPLQLSDLKKLQPYFLKSFD 261
           KPLQLSDL KLQPYFLKS D
Sbjct: 153 KPLQLSDLDKLQPYFLKSLD 172


>XP_003523433.1 PREDICTED: two-component response regulator ORR9-like [Glycine max]
           KHN35505.1 Two-component response regulator ARR9
           [Glycine soja] KRH64643.1 hypothetical protein
           GLYMA_04G247800 [Glycine max]
          Length = 235

 Score =  151 bits (382), Expect = 5e-43
 Identities = 74/80 (92%), Positives = 76/80 (95%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           GIKVNLIMTDYCMPGMSGYDLLKR+K SSWKDVPV+IMSSENVPSRISMCLE GAEEFLL
Sbjct: 93  GIKVNLIMTDYCMPGMSGYDLLKRVKGSSWKDVPVVIMSSENVPSRISMCLEEGAEEFLL 152

Query: 320 KPLQLSDLKKLQPYFLKSFD 261
           KPLQLSDL KLQPYFLKS D
Sbjct: 153 KPLQLSDLDKLQPYFLKSVD 172


>ACU23880.1 unknown [Glycine max]
          Length = 235

 Score =  151 bits (382), Expect = 5e-43
 Identities = 74/80 (92%), Positives = 76/80 (95%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           GIKVNLIMTDYCMPGMSGYDLLKR+K SSWKDVPV+IMSSENVPSRISMCLE GAEEFLL
Sbjct: 93  GIKVNLIMTDYCMPGMSGYDLLKRVKGSSWKDVPVVIMSSENVPSRISMCLEEGAEEFLL 152

Query: 320 KPLQLSDLKKLQPYFLKSFD 261
           KPLQLSDL KLQPYFLKS D
Sbjct: 153 KPLQLSDLDKLQPYFLKSVD 172


>AFK44001.1 unknown [Medicago truncatula]
          Length = 235

 Score =  151 bits (381), Expect = 7e-43
 Identities = 75/87 (86%), Positives = 80/87 (91%), Gaps = 4/87 (4%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           G KVNLIMTDYCMPGMSGYDLLKR+K SSWKDVPV++MSSENVPSRISMCLE GAEEFL+
Sbjct: 92  GNKVNLIMTDYCMPGMSGYDLLKRVKGSSWKDVPVVVMSSENVPSRISMCLEEGAEEFLM 151

Query: 320 KPLQLSDLKKLQPYFLK----SFDEQE 252
           KPLQLSDL+KLQPYFLK    SFDEQE
Sbjct: 152 KPLQLSDLQKLQPYFLKSLENSFDEQE 178


>XP_003526642.1 PREDICTED: two-component response regulator ORR9-like [Glycine max]
           KRH53261.1 hypothetical protein GLYMA_06G114900 [Glycine
           max]
          Length = 232

 Score =  150 bits (380), Expect = 1e-42
 Identities = 73/80 (91%), Positives = 76/80 (95%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           GIKVNLIMTDYCMPGMSGYDLLKR+K SSWKDVPV+IMSSENVPSRISMCLE GA+EFLL
Sbjct: 92  GIKVNLIMTDYCMPGMSGYDLLKRVKGSSWKDVPVVIMSSENVPSRISMCLEEGAQEFLL 151

Query: 320 KPLQLSDLKKLQPYFLKSFD 261
           KPLQLSDL KLQPYFLKS D
Sbjct: 152 KPLQLSDLDKLQPYFLKSLD 171


>XP_014500206.1 PREDICTED: two-component response regulator ORR9-like [Vigna
           radiata var. radiata]
          Length = 225

 Score =  150 bits (379), Expect = 1e-42
 Identities = 73/82 (89%), Positives = 77/82 (93%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           GIKVNLIMTDYCMPGMSGYDLLKR+K SSWKDVPV+IMSSENVPSRISMCLEGGAEEFLL
Sbjct: 92  GIKVNLIMTDYCMPGMSGYDLLKRVKGSSWKDVPVVIMSSENVPSRISMCLEGGAEEFLL 151

Query: 320 KPLQLSDLKKLQPYFLKSFDEQ 255
           KPLQLSDL KLQ YFLKS + +
Sbjct: 152 KPLQLSDLDKLQSYFLKSCENE 173


>XP_017422043.1 PREDICTED: two-component response regulator ORR9-like [Vigna
           angularis] KOM41557.1 hypothetical protein
           LR48_Vigan04g175500 [Vigna angularis] BAT78661.1
           hypothetical protein VIGAN_02137000 [Vigna angularis
           var. angularis]
          Length = 231

 Score =  150 bits (379), Expect = 1e-42
 Identities = 73/80 (91%), Positives = 76/80 (95%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           GIKVNLIMTDYCMPGMSGYDLLKR+K SSWKDVPV+IMSSENVPSRISMCLEGGAEEFLL
Sbjct: 96  GIKVNLIMTDYCMPGMSGYDLLKRVKGSSWKDVPVVIMSSENVPSRISMCLEGGAEEFLL 155

Query: 320 KPLQLSDLKKLQPYFLKSFD 261
           KPLQLSDL KLQ YFLKS +
Sbjct: 156 KPLQLSDLDKLQSYFLKSLE 175


>XP_007137437.1 hypothetical protein PHAVU_009G127000g [Phaseolus vulgaris]
           ESW09431.1 hypothetical protein PHAVU_009G127000g
           [Phaseolus vulgaris]
          Length = 237

 Score =  150 bits (379), Expect = 2e-42
 Identities = 73/80 (91%), Positives = 76/80 (95%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           GIKVNLIMTDYCMPGMSGYDLLKR+K SSWKDVPV+IMSSENVPSRISMCLEGGAEEFLL
Sbjct: 94  GIKVNLIMTDYCMPGMSGYDLLKRVKGSSWKDVPVVIMSSENVPSRISMCLEGGAEEFLL 153

Query: 320 KPLQLSDLKKLQPYFLKSFD 261
           KPLQLSDL KLQ YFLKS +
Sbjct: 154 KPLQLSDLDKLQSYFLKSLE 173


>XP_019415488.1 PREDICTED: two-component response regulator ORR9-like [Lupinus
           angustifolius]
          Length = 230

 Score =  150 bits (378), Expect = 2e-42
 Identities = 74/86 (86%), Positives = 80/86 (93%), Gaps = 3/86 (3%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           G+KVNLIMTDYCMPGMSGYDLLKR+K SSWKDVPV++MSSENVPSRISMCLE GAEEFLL
Sbjct: 93  GMKVNLIMTDYCMPGMSGYDLLKRVKGSSWKDVPVVVMSSENVPSRISMCLEEGAEEFLL 152

Query: 320 KPLQLSDLKKLQPYFLKSFD---EQE 252
           KPLQLSDLKKLQPYF+KS +   EQE
Sbjct: 153 KPLQLSDLKKLQPYFMKSHNNSCEQE 178


>XP_012078259.1 PREDICTED: two-component response regulator ARR8-like [Jatropha
           curcas] KDP32814.1 hypothetical protein JCGZ_12106
           [Jatropha curcas]
          Length = 222

 Score =  149 bits (377), Expect = 2e-42
 Identities = 69/81 (85%), Positives = 78/81 (96%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           G+KVNLIMTDYCMPGMSGYDLLKR+K SSWKDVPV++MSSEN+PSRISMCLEGGAEEFLL
Sbjct: 86  GMKVNLIMTDYCMPGMSGYDLLKRVKGSSWKDVPVVVMSSENIPSRISMCLEGGAEEFLL 145

Query: 320 KPLQLSDLKKLQPYFLKSFDE 258
           KPLQLSD++KLQP+ LKS D+
Sbjct: 146 KPLQLSDMQKLQPHLLKSLDQ 166


>XP_016163494.1 PREDICTED: two-component response regulator ORR9-like, partial
           [Arachis ipaensis]
          Length = 213

 Score =  149 bits (376), Expect = 2e-42
 Identities = 71/80 (88%), Positives = 75/80 (93%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           G+KVNLIMTDYCMPGMSGYDLLKR+K SSWKDVPV+IMSSENVPSRI+MCLEGGAEEFLL
Sbjct: 103 GMKVNLIMTDYCMPGMSGYDLLKRVKGSSWKDVPVVIMSSENVPSRITMCLEGGAEEFLL 162

Query: 320 KPLQLSDLKKLQPYFLKSFD 261
           KPLQ  DLKKLQPYFLK  D
Sbjct: 163 KPLQSEDLKKLQPYFLKPLD 182


>OAY58284.1 hypothetical protein MANES_02G164700 [Manihot esculenta]
          Length = 229

 Score =  149 bits (377), Expect = 3e-42
 Identities = 69/81 (85%), Positives = 78/81 (96%)
 Frame = -1

Query: 500 GIKVNLIMTDYCMPGMSGYDLLKRIKRSSWKDVPVMIMSSENVPSRISMCLEGGAEEFLL 321
           G+KVNLIMTDYCMPGMSGYDLLKR+K SSWKDVPV++MSSEN+PSRISMCLEGGAEEFLL
Sbjct: 101 GMKVNLIMTDYCMPGMSGYDLLKRVKGSSWKDVPVVVMSSENIPSRISMCLEGGAEEFLL 160

Query: 320 KPLQLSDLKKLQPYFLKSFDE 258
           KPLQLSD++KLQP+ LKS D+
Sbjct: 161 KPLQLSDMEKLQPHLLKSLDQ 181