BLASTX nr result
ID: Glycyrrhiza28_contig00037559
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Glycyrrhiza28_contig00037559
(294 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_015953847.1 PREDICTED: callose synthase 12-like [Arachis dura... 189 6e-54
XP_014493833.1 PREDICTED: callose synthase 12 [Vigna radiata var... 189 8e-54
XP_017432990.1 PREDICTED: callose synthase 12 [Vigna angularis] ... 189 8e-54
XP_016188280.1 PREDICTED: callose synthase 12-like [Arachis ipae... 189 9e-54
XP_003607458.1 callose synthase-like protein [Medicago truncatul... 187 3e-53
XP_007132658.1 hypothetical protein PHAVU_011G113800g [Phaseolus... 186 1e-52
XP_012572668.1 PREDICTED: callose synthase 12-like isoform X1 [C... 186 1e-52
OAY34059.1 hypothetical protein MANES_13G146600 [Manihot esculen... 185 1e-52
XP_002517915.1 PREDICTED: callose synthase 12 [Ricinus communis]... 184 3e-52
XP_011086366.1 PREDICTED: callose synthase 12 [Sesamum indicum] 184 4e-52
XP_019413240.1 PREDICTED: callose synthase 12-like [Lupinus angu... 182 1e-51
OIV99243.1 hypothetical protein TanjilG_06548 [Lupinus angustifo... 182 1e-51
XP_012088285.1 PREDICTED: callose synthase 12 [Jatropha curcas] ... 182 2e-51
XP_010097906.1 Callose synthase 12 [Morus notabilis] EXB72969.1 ... 181 6e-51
OAY22435.1 hypothetical protein MANES_S000600 [Manihot esculenta] 181 6e-51
XP_015871227.1 PREDICTED: callose synthase 12-like [Ziziphus juj... 180 1e-50
XP_015870874.1 PREDICTED: callose synthase 12-like [Ziziphus juj... 180 1e-50
XP_015870843.1 PREDICTED: callose synthase 12-like [Ziziphus juj... 180 1e-50
XP_006376493.1 hypothetical protein POPTR_0013s134901g, partial ... 178 1e-50
KHN31989.1 Callose synthase 12 [Glycine soja] 180 1e-50
>XP_015953847.1 PREDICTED: callose synthase 12-like [Arachis duranensis]
Length = 1745
Score = 189 bits (480), Expect = 6e-54
Identities = 94/97 (96%), Positives = 95/97 (97%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
LPVVLIYLMDIQIWYSIYSSF GAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE
Sbjct: 545 LPVVLIYLMDIQIWYSIYSSFVGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 604
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLNARGTLKSKFKDAIHRLKLRYGLGRP+ KLESNQ
Sbjct: 605 QLLNARGTLKSKFKDAIHRLKLRYGLGRPFKKLESNQ 641
>XP_014493833.1 PREDICTED: callose synthase 12 [Vigna radiata var. radiata]
Length = 1769
Score = 189 bits (479), Expect = 8e-54
Identities = 94/97 (96%), Positives = 94/97 (96%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
LPVVLIYLMDIQIWYSIYSSFAGA VGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE
Sbjct: 543 LPVVLIYLMDIQIWYSIYSSFAGAGVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 602
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLN RGTLKSKFKDAIHRLKLRYGLGRPY KLESNQ
Sbjct: 603 QLLNTRGTLKSKFKDAIHRLKLRYGLGRPYRKLESNQ 639
>XP_017432990.1 PREDICTED: callose synthase 12 [Vigna angularis] KOM50310.1
hypothetical protein LR48_Vigan08g113700 [Vigna
angularis] BAT90130.1 hypothetical protein
VIGAN_06131300 [Vigna angularis var. angularis]
Length = 1769
Score = 189 bits (479), Expect = 8e-54
Identities = 94/97 (96%), Positives = 94/97 (96%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
LPVVLIYLMDIQIWYSIYSSFAGA VGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE
Sbjct: 543 LPVVLIYLMDIQIWYSIYSSFAGAGVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 602
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLN RGTLKSKFKDAIHRLKLRYGLGRPY KLESNQ
Sbjct: 603 QLLNTRGTLKSKFKDAIHRLKLRYGLGRPYRKLESNQ 639
>XP_016188280.1 PREDICTED: callose synthase 12-like [Arachis ipaensis]
Length = 1701
Score = 189 bits (479), Expect = 9e-54
Identities = 93/97 (95%), Positives = 95/97 (97%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
LPVVLIYLMDIQIWYSIYSSF GAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE
Sbjct: 476 LPVVLIYLMDIQIWYSIYSSFVGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 535
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLNARGTLKSKFKDA+HRLKLRYGLGRP+ KLESNQ
Sbjct: 536 QLLNARGTLKSKFKDAVHRLKLRYGLGRPFKKLESNQ 572
>XP_003607458.1 callose synthase-like protein [Medicago truncatula] AES89655.1
callose synthase-like protein [Medicago truncatula]
Length = 1815
Score = 187 bits (475), Expect = 3e-53
Identities = 93/97 (95%), Positives = 94/97 (96%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
+PVVLIYLMDIQIWYSIYSS AGA VGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE
Sbjct: 537 IPVVLIYLMDIQIWYSIYSSLAGAGVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 596
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLNARGTLKSKFKDAIHRLKLRYGLGRPY KLESNQ
Sbjct: 597 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYRKLESNQ 633
>XP_007132658.1 hypothetical protein PHAVU_011G113800g [Phaseolus vulgaris]
ESW04652.1 hypothetical protein PHAVU_011G113800g
[Phaseolus vulgaris]
Length = 1769
Score = 186 bits (471), Expect = 1e-52
Identities = 93/97 (95%), Positives = 93/97 (95%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
LPVVLIYLMDIQIWYSIYSSFAGA VGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE
Sbjct: 543 LPVVLIYLMDIQIWYSIYSSFAGAGVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 602
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLN R TLKSKFKDAIHRLKLRYGLGRPY KLESNQ
Sbjct: 603 QLLNTRRTLKSKFKDAIHRLKLRYGLGRPYRKLESNQ 639
>XP_012572668.1 PREDICTED: callose synthase 12-like isoform X1 [Cicer arietinum]
XP_012572669.1 PREDICTED: callose synthase 12-like
isoform X2 [Cicer arietinum] XP_012572670.1 PREDICTED:
callose synthase 12-like isoform X3 [Cicer arietinum]
XP_012572671.1 PREDICTED: callose synthase 12-like
isoform X1 [Cicer arietinum]
Length = 1766
Score = 186 bits (471), Expect = 1e-52
Identities = 92/97 (94%), Positives = 94/97 (96%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
+PV+LIYLMDIQIWYSIYSSFAGA VGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE
Sbjct: 540 VPVLLIYLMDIQIWYSIYSSFAGAVVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 599
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLNA GTLKSKFKDAIHRLKLRYGLGRPY KLESNQ
Sbjct: 600 QLLNATGTLKSKFKDAIHRLKLRYGLGRPYRKLESNQ 636
>OAY34059.1 hypothetical protein MANES_13G146600 [Manihot esculenta] OAY34060.1
hypothetical protein MANES_13G146600 [Manihot esculenta]
Length = 1773
Score = 185 bits (470), Expect = 1e-52
Identities = 91/97 (93%), Positives = 95/97 (97%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
LPVVLIYLMD+QIWYSIYSSFAGAAVGLF HLGEIRN+QQL+LRFQFFASAIQFNLMPEE
Sbjct: 541 LPVVLIYLMDLQIWYSIYSSFAGAAVGLFEHLGEIRNIQQLRLRFQFFASAIQFNLMPEE 600
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLNARGTLKSKFKDAIHRLKLRYGLGRP+ KLESNQ
Sbjct: 601 QLLNARGTLKSKFKDAIHRLKLRYGLGRPFKKLESNQ 637
>XP_002517915.1 PREDICTED: callose synthase 12 [Ricinus communis] EEF44433.1
transferase, transferring glycosyl groups, putative
[Ricinus communis]
Length = 1767
Score = 184 bits (468), Expect = 3e-52
Identities = 90/97 (92%), Positives = 94/97 (96%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
LPVV IYLMD+QIWY+IYSSF GAAVGLFAHLGEIRN+QQL+LRFQFFASAIQFNLMPEE
Sbjct: 539 LPVVFIYLMDLQIWYAIYSSFVGAAVGLFAHLGEIRNIQQLRLRFQFFASAIQFNLMPEE 598
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLNARGTLKSKFKDAIHRLKLRYGLGRPY KLESNQ
Sbjct: 599 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYKKLESNQ 635
>XP_011086366.1 PREDICTED: callose synthase 12 [Sesamum indicum]
Length = 1771
Score = 184 bits (467), Expect = 4e-52
Identities = 91/97 (93%), Positives = 93/97 (95%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
LPVVLIYLMDIQIWYSIYSSF GAAVGLF HLGEIRNMQQL+LRFQFFASAIQFNLMPEE
Sbjct: 533 LPVVLIYLMDIQIWYSIYSSFVGAAVGLFDHLGEIRNMQQLRLRFQFFASAIQFNLMPEE 592
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLNARGT KSKFKDAIHRLKLRYGLGRP+ KLESNQ
Sbjct: 593 QLLNARGTFKSKFKDAIHRLKLRYGLGRPFKKLESNQ 629
>XP_019413240.1 PREDICTED: callose synthase 12-like [Lupinus angustifolius]
Length = 1768
Score = 182 bits (463), Expect = 1e-51
Identities = 89/97 (91%), Positives = 93/97 (95%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
+PVVL+YLMD QIWYSIYSSF GAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE
Sbjct: 542 IPVVLVYLMDTQIWYSIYSSFVGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 601
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLNARGTL SKFK+AIHRLKLRYGLGRPY K+ESNQ
Sbjct: 602 QLLNARGTLASKFKNAIHRLKLRYGLGRPYKKIESNQ 638
>OIV99243.1 hypothetical protein TanjilG_06548 [Lupinus angustifolius]
Length = 1741
Score = 182 bits (463), Expect = 1e-51
Identities = 89/97 (91%), Positives = 93/97 (95%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
+PVVL+YLMD QIWYSIYSSF GAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE
Sbjct: 542 IPVVLVYLMDTQIWYSIYSSFVGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 601
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLNARGTL SKFK+AIHRLKLRYGLGRPY K+ESNQ
Sbjct: 602 QLLNARGTLASKFKNAIHRLKLRYGLGRPYKKIESNQ 638
>XP_012088285.1 PREDICTED: callose synthase 12 [Jatropha curcas] KDP24129.1
hypothetical protein JCGZ_25786 [Jatropha curcas]
Length = 1771
Score = 182 bits (462), Expect = 2e-51
Identities = 88/97 (90%), Positives = 94/97 (96%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
LPVV +Y+MD+QIWYSIYSSF GAAVGLF HLGEIRN+QQL+LRFQFFASAIQFNLMPEE
Sbjct: 541 LPVVFVYVMDLQIWYSIYSSFVGAAVGLFEHLGEIRNIQQLRLRFQFFASAIQFNLMPEE 600
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLNARGTLKSKFKDAIHRLKLRYGLG+PYSKLESNQ
Sbjct: 601 QLLNARGTLKSKFKDAIHRLKLRYGLGQPYSKLESNQ 637
>XP_010097906.1 Callose synthase 12 [Morus notabilis] EXB72969.1 Callose synthase
12 [Morus notabilis]
Length = 1774
Score = 181 bits (458), Expect = 6e-51
Identities = 87/97 (89%), Positives = 94/97 (96%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
LPVVLIYLMD+QIWYSIYSSF GAAVGLF+HLGEIRN+QQL+LRFQFFASAIQFNLMPEE
Sbjct: 544 LPVVLIYLMDLQIWYSIYSSFVGAAVGLFSHLGEIRNLQQLRLRFQFFASAIQFNLMPEE 603
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLNARGTL++KFKDAIHRLKLRYG G+PY KLESNQ
Sbjct: 604 QLLNARGTLRNKFKDAIHRLKLRYGFGQPYRKLESNQ 640
>OAY22435.1 hypothetical protein MANES_S000600 [Manihot esculenta]
Length = 1772
Score = 181 bits (458), Expect = 6e-51
Identities = 89/97 (91%), Positives = 92/97 (94%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
LPVVLIYLMD+QIWYSIYSSF GAAVGLFAHLGEIRN+QQL+LRFQFFASAIQFNLMPEE
Sbjct: 541 LPVVLIYLMDLQIWYSIYSSFVGAAVGLFAHLGEIRNIQQLRLRFQFFASAIQFNLMPEE 600
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLNARG LKSK KDAIHRLKLRYGLGRPY KLE NQ
Sbjct: 601 QLLNARGVLKSKLKDAIHRLKLRYGLGRPYRKLEPNQ 637
>XP_015871227.1 PREDICTED: callose synthase 12-like [Ziziphus jujuba]
Length = 1773
Score = 180 bits (456), Expect = 1e-50
Identities = 88/97 (90%), Positives = 92/97 (94%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
LPVVLIYLMD+QIWYSIYSSF GA VGLFAHLGEIRN+QQL+LRFQFFASAIQFNLMPEE
Sbjct: 544 LPVVLIYLMDLQIWYSIYSSFVGAGVGLFAHLGEIRNIQQLRLRFQFFASAIQFNLMPEE 603
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLN RGTLK+KFKDAI RLKLRYGLGRPY KLESNQ
Sbjct: 604 QLLNTRGTLKNKFKDAIRRLKLRYGLGRPYKKLESNQ 640
>XP_015870874.1 PREDICTED: callose synthase 12-like [Ziziphus jujuba]
XP_015870875.1 PREDICTED: callose synthase 12-like
[Ziziphus jujuba] XP_015870940.1 PREDICTED: callose
synthase 12-like [Ziziphus jujuba] XP_015870942.1
PREDICTED: callose synthase 12-like [Ziziphus jujuba]
Length = 1773
Score = 180 bits (456), Expect = 1e-50
Identities = 88/97 (90%), Positives = 92/97 (94%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
LPVVLIYLMD+QIWYSIYSSF GA VGLFAHLGEIRN+QQL+LRFQFFASAIQFNLMPEE
Sbjct: 544 LPVVLIYLMDLQIWYSIYSSFVGAGVGLFAHLGEIRNIQQLRLRFQFFASAIQFNLMPEE 603
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLN RGTLK+KFKDAI RLKLRYGLGRPY KLESNQ
Sbjct: 604 QLLNTRGTLKNKFKDAIRRLKLRYGLGRPYKKLESNQ 640
>XP_015870843.1 PREDICTED: callose synthase 12-like [Ziziphus jujuba]
XP_015870844.1 PREDICTED: callose synthase 12-like
[Ziziphus jujuba] XP_015870845.1 PREDICTED: callose
synthase 12-like [Ziziphus jujuba]
Length = 1773
Score = 180 bits (456), Expect = 1e-50
Identities = 88/97 (90%), Positives = 92/97 (94%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
LPVVLIYLMD+QIWYSIYSSF GA VGLFAHLGEIRN+QQL+LRFQFFASAIQFNLMPEE
Sbjct: 544 LPVVLIYLMDLQIWYSIYSSFVGAGVGLFAHLGEIRNIQQLRLRFQFFASAIQFNLMPEE 603
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLN RGTLK+KFKDAI RLKLRYGLGRPY KLESNQ
Sbjct: 604 QLLNTRGTLKNKFKDAIRRLKLRYGLGRPYKKLESNQ 640
>XP_006376493.1 hypothetical protein POPTR_0013s134901g, partial [Populus
trichocarpa] ERP54290.1 hypothetical protein
POPTR_0013s134901g, partial [Populus trichocarpa]
Length = 708
Score = 178 bits (452), Expect = 1e-50
Identities = 86/97 (88%), Positives = 91/97 (93%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
LPVVLIYLMD+QIWY+IYSSF GA VGLF HLGEIRN+QQL+LRFQFFASAIQFNLMPEE
Sbjct: 540 LPVVLIYLMDLQIWYAIYSSFVGAGVGLFQHLGEIRNIQQLRLRFQFFASAIQFNLMPEE 599
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLNARGT+KSKFKDAIHRLKLRYG G PY KLESNQ
Sbjct: 600 QLLNARGTIKSKFKDAIHRLKLRYGFGHPYKKLESNQ 636
>KHN31989.1 Callose synthase 12 [Glycine soja]
Length = 1669
Score = 180 bits (456), Expect = 1e-50
Identities = 90/97 (92%), Positives = 91/97 (93%)
Frame = -2
Query: 293 LPVVLIYLMDIQIWYSIYSSFAGAAVGLFAHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 114
LPVVLIYLMDIQIWYSIYSSFAGA VGL HLGEIRNMQQLKLRFQFFASAIQFNLMPEE
Sbjct: 443 LPVVLIYLMDIQIWYSIYSSFAGAIVGLLEHLGEIRNMQQLKLRFQFFASAIQFNLMPEE 502
Query: 113 QLLNARGTLKSKFKDAIHRLKLRYGLGRPYSKLESNQ 3
QLLN RGTLKS+FKDAI RLKLRYGLGRPY KLESNQ
Sbjct: 503 QLLNTRGTLKSRFKDAIRRLKLRYGLGRPYRKLESNQ 539