BLASTX nr result
ID: Gardenia21_contig00012918
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Gardenia21_contig00012918
(1559 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CDP11973.1| unnamed protein product [Coffea canephora] 352 e-112
emb|CDP11972.1| unnamed protein product [Coffea canephora] 341 e-112
ref|XP_002284916.2| PREDICTED: uncharacterized acetyltransferase... 319 e-104
emb|CAN78155.1| hypothetical protein VITISV_032623 [Vitis vinifera] 317 e-104
ref|XP_006374905.1| transferase family protein [Populus trichoca... 322 e-103
ref|XP_006374906.1| anthranilate N-hydroxycinamoyl/bensoiltransf... 320 e-103
ref|XP_011029557.1| PREDICTED: uncharacterized acetyltransferase... 317 e-101
ref|XP_012068314.1| PREDICTED: uncharacterized acetyltransferase... 321 e-101
emb|CDP06257.1| unnamed protein product [Coffea canephora] 311 e-101
emb|CDP06258.1| unnamed protein product [Coffea canephora] 312 e-100
ref|XP_006473294.1| PREDICTED: uncharacterized acetyltransferase... 305 2e-99
gb|KDO84111.1| hypothetical protein CISIN_1g044173mg [Citrus sin... 305 3e-99
ref|XP_012066805.1| PREDICTED: uncharacterized acetyltransferase... 315 4e-99
ref|XP_012452172.1| PREDICTED: uncharacterized acetyltransferase... 307 1e-98
ref|XP_012066803.1| PREDICTED: uncharacterized acetyltransferase... 318 1e-98
ref|XP_007224078.1| hypothetical protein PRUPE_ppa015472mg [Prun... 308 2e-97
ref|XP_007221613.1| hypothetical protein PRUPE_ppb015328mg [Prun... 303 3e-97
ref|XP_002510186.1| Anthranilate N-benzoyltransferase protein, p... 300 3e-97
ref|XP_008220248.1| PREDICTED: uncharacterized acetyltransferase... 303 6e-97
ref|XP_010110810.1| putative acetyltransferase [Morus notabilis]... 305 8e-97
>emb|CDP11973.1| unnamed protein product [Coffea canephora]
Length = 454
Score = 352 bits (902), Expect(2) = e-112
Identities = 178/298 (59%), Positives = 219/298 (73%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M SP I+ +SQCFIKPK+TPE +KQP+YLS D+ Y+Q+G +FAKP FD E
Sbjct: 1 MSSPGIQHISQCFIKPKNTPEEAKQPIYLSQWDLAMACANYIQKGLLFAKPPAFDR---E 57
Query: 1333 NQLQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASLD 1154
NQ+++ +EKLK+SL+ L HFYPLAGRLAT K +ENP + YVDCN PGARFVHASL+
Sbjct: 58 NQMEDLLEKLKDSLALALDHFYPLAGRLATLK-QENPPIYSIYVDCNNSPGARFVHASLN 116
Query: 1153 LTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGDG 974
TID I+SP +P+I++S FDH R +N+DGHT LLTIQVTELIDGIFMGCS NHM+ DG
Sbjct: 117 STIDDILSPVDVPKIVQSFFDHDRAINHDGHTRPLLTIQVTELIDGIFMGCSANHMIVDG 176
Query: 973 TSLWHFLASWSEIFKAEGKSIAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTITRDE 794
TS WHF +WSEIF A+G+ IAISRPPIH+RWF G P +SLP + R E
Sbjct: 177 TSYWHFFNTWSEIFNAKGQKIAISRPPIHKRWFLEG-HGPILSLPFTHH---DQFVRRHE 232
Query: 793 ADTSPANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRCLLRAWNFP 620
A ++ER+FHFSSES+ KLKAKAN E+DTT+ISS QAL+AH+WRC+ R N P
Sbjct: 233 APQ-----MRERVFHFSSESLAKLKAKANAESDTTKISSLQALAAHVWRCITRTRNLP 285
Score = 83.6 bits (205), Expect(2) = e-112
Identities = 50/129 (38%), Positives = 70/129 (54%), Gaps = 13/129 (10%)
Frame = -3
Query: 591 AIGGCGKLLENGLGWTAWLLHQAVVNK--EMSYEWINSRLQSLSLPRNTSVTKPGFIVQT 418
A G+LL+ GLGW++ LH AV N E+ W+ S LQS + + P ++
Sbjct: 320 ATAAAGELLDRGLGWSSSKLHLAVHNHTDEIVRNWVESWLQSPVIYQAAEFIDPCSVLMG 379
Query: 417 GSPRFDLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLES 271
SPRF+++ +FGLGK VA R+G A G D+ ICL PH+M+ LES
Sbjct: 380 SSPRFNMYGSEFGLGKAVAIRSGYANKFDGKVSSFPGVEGGGSMDLEICLRPHSMSLLES 439
Query: 270 DKEFMETVS 244
D+EFM V+
Sbjct: 440 DEEFMGAVT 448
>emb|CDP11972.1| unnamed protein product [Coffea canephora]
Length = 455
Score = 341 bits (874), Expect(2) = e-112
Identities = 174/298 (58%), Positives = 217/298 (72%), Gaps = 1/298 (0%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M SP I +SQCFIKPK+TPE +KQP+YLS D+ V V Y+Q+G +FAKP FD E
Sbjct: 1 MSSPGIHHISQCFIKPKNTPEEAKQPIYLSQWDLAMVCVNYIQKGLLFAKPPAFDR---E 57
Query: 1333 NQLQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASLD 1154
NQ+++ +EKLK+SL+ L HFYPLAGRLAT K +ENP+ + YVDCN PGA VHASL+
Sbjct: 58 NQMEDLLEKLKDSLALALDHFYPLAGRLATLK-QENPAIYSIYVDCNNSPGASLVHASLN 116
Query: 1153 LTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGDG 974
TID I+SP +P+I++S FDH R +N+DGHT LLTIQVTEL DGIF+GCS+NHM+ DG
Sbjct: 117 STIDDILSPIDVPEIVQSFFDHDRAINHDGHTRPLLTIQVTELTDGIFIGCSINHMIVDG 176
Query: 973 TSLWHFLASWSEIFKAEGKSIAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTITRDE 794
T+ WHF +WSEIF A+G+ AISRPPIH+ WFP G P +SLP + R E
Sbjct: 177 TAFWHFFNTWSEIFSAKGQKTAISRPPIHKHWFPEG-HGPILSLPFTHH---DQFVRRHE 232
Query: 793 ADTSPANVIKERIFHFSSESVRKLKAKANFEAD-TTEISSFQALSAHLWRCLLRAWNF 623
A ++ER+FHFSSES+ KLKAKAN E+ TT+ISS QALSAH+WRC+ R NF
Sbjct: 233 APQ-----MRERVFHFSSESLAKLKAKANAESSHTTKISSLQALSAHVWRCITRTRNF 285
Score = 93.6 bits (231), Expect(2) = e-112
Identities = 55/129 (42%), Positives = 73/129 (56%), Gaps = 13/129 (10%)
Frame = -3
Query: 591 AIGGCGKLLENGLGWTAWLLHQAVVNK--EMSYEWINSRLQSLSLPRNTSVTKPGFIVQT 418
A G+LL+ GLGW+AW LH AV N E+ +W+ S LQS + + P I+
Sbjct: 321 AAAAAGELLDRGLGWSAWKLHLAVHNHTDEIVRDWVESWLQSRFIYQLAQFFDPCSIMMG 380
Query: 417 GSPRFDLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLES 271
SPRF+++ +FGLGK VA R+G A G D+ ICL PH M+ LES
Sbjct: 381 SSPRFNMYGNEFGLGKAVAIRSGYANKFDGKVSLYPGIEGGGSMDLEICLLPHYMSLLES 440
Query: 270 DKEFMETVS 244
D+EFMETV+
Sbjct: 441 DEEFMETVT 449
>ref|XP_002284916.2| PREDICTED: uncharacterized acetyltransferase At3g50280-like [Vitis
vinifera]
Length = 454
Score = 319 bits (818), Expect(2) = e-104
Identities = 160/300 (53%), Positives = 218/300 (72%), Gaps = 2/300 (0%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M +P +R +S+CFIKPK T E +K+P YL+P D+ + V Y+Q+G +F+KP D D +
Sbjct: 1 MATPTVRLISECFIKPKFTSEETKEPCYLTPWDLAMLSVNYIQKGLLFSKPP--DVDDPQ 58
Query: 1333 NQLQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASLD 1154
N + +++LK+SL+ TL HFYPLAGRLAT KKE++P +VDCN PGA+F+HA+ D
Sbjct: 59 NFMATLLDRLKDSLALTLDHFYPLAGRLAT-KKEDSPPSYVVFVDCNNSPGAKFIHAAAD 117
Query: 1153 LTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGDG 974
+TI I+SP Y+PQ+++S FDH R++N+DGHT SLL+IQVTEL+DGIF+GCS+NH + DG
Sbjct: 118 MTISDILSPIYVPQVIQSFFDHDRVINHDGHTLSLLSIQVTELVDGIFIGCSINHSMVDG 177
Query: 973 TSLWHFLASWSEIFKAEGKS--IAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTITR 800
TS WHF +WSE+F A+ K+ I +SRPPI +RWFP G P ++LP I+R
Sbjct: 178 TSFWHFFNAWSEVFTAQEKNSPITLSRPPILKRWFPDG-YGPIINLPFTHH---DEFISR 233
Query: 799 DEADTSPANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRCLLRAWNFP 620
EA V++ERIFHFSSES+ KLKAKAN + ++ +ISSFQALSA +WR + RA FP
Sbjct: 234 FEAP-----VLRERIFHFSSESIAKLKAKANAQCNSNKISSFQALSALVWRSITRARCFP 288
Score = 90.5 bits (223), Expect(2) = e-104
Identities = 52/128 (40%), Positives = 73/128 (57%), Gaps = 13/128 (10%)
Frame = -3
Query: 588 IGGCGKLLENGLGWTAWLLHQAVV--NKEMSYEWINSRLQSLSLPRNTSVTKPGFIVQTG 415
I G+LLE+ LGW AWLLHQAVV + W+ S QS + + P ++
Sbjct: 324 IATAGELLEHDLGWAAWLLHQAVVGHTDKAVRGWLESWFQSHFIYQLGLFFDPNSVMMGS 383
Query: 414 SPRFDLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLESD 268
SPRF+ + I+FGLGK +A R+G A G D+ +CLPP++M++LES+
Sbjct: 384 SPRFNKYGIEFGLGKGLAVRSGYAHKFDGTVSCYPGREGGGSIDLEVCLPPNSMSALESN 443
Query: 267 KEFMETVS 244
+EFME VS
Sbjct: 444 QEFMEAVS 451
>emb|CAN78155.1| hypothetical protein VITISV_032623 [Vitis vinifera]
Length = 602
Score = 317 bits (813), Expect(2) = e-104
Identities = 159/300 (53%), Positives = 218/300 (72%), Gaps = 2/300 (0%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M +P +R +S+CFIKPK T E + +P YL+P D+ + V Y+Q+G +F+KP D D +
Sbjct: 80 MATPTVRLISECFIKPKFTSEETNEPCYLTPWDLAMLSVNYIQKGLLFSKPP--DVDDPQ 137
Query: 1333 NQLQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASLD 1154
N + ++KLK+SL+ TL HFYPLAGRLAT+K++ +PS + F VDCN PG +F+HA+ D
Sbjct: 138 NFMATLLDKLKDSLALTLDHFYPLAGRLATKKEDSSPSYVVF-VDCNNSPGLKFIHAAAD 196
Query: 1153 LTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGDG 974
+TI I+SP Y+PQ+++S FDH R++N+DG T SLL+IQ+TEL+DGIF+GCS+NH + DG
Sbjct: 197 MTISDILSPIYVPQVIQSFFDHDRVINHDGRTLSLLSIQITELVDGIFIGCSINHSMVDG 256
Query: 973 TSLWHFLASWSEIFKAEGK--SIAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTITR 800
TS WHF +WSE+F A+GK SI +SRPPI +RWFP G P ++LP I+R
Sbjct: 257 TSFWHFFNAWSEVFTAQGKNSSITLSRPPILKRWFPDG-YGPIINLPFTHH---DEFISR 312
Query: 799 DEADTSPANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRCLLRAWNFP 620
EA V++ERIFHFSSES+ KLKAKAN + ++ +ISSFQALSA +WR + RA FP
Sbjct: 313 FEAP-----VLRERIFHFSSESIAKLKAKANAQCNSNKISSFQALSALVWRSITRARCFP 367
Score = 90.5 bits (223), Expect(2) = e-104
Identities = 52/128 (40%), Positives = 73/128 (57%), Gaps = 13/128 (10%)
Frame = -3
Query: 588 IGGCGKLLENGLGWTAWLLHQAVV--NKEMSYEWINSRLQSLSLPRNTSVTKPGFIVQTG 415
I G+LLE+ LGW AWLLHQAVV + W+ S QS + + P ++
Sbjct: 403 IATAGELLEHDLGWAAWLLHQAVVGHTDKAVRGWLESWFQSHFIYQLGLFFDPNSVMMGS 462
Query: 414 SPRFDLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLESD 268
SPRF+ + I+FGLGK +A R+G A G D+ +CLPP++M++LES+
Sbjct: 463 SPRFNKYGIEFGLGKGLAVRSGYAHKFDGKVSCYPGREGGGSIDLEVCLPPNSMSALESN 522
Query: 267 KEFMETVS 244
+EFME VS
Sbjct: 523 QEFMEAVS 530
>ref|XP_006374905.1| transferase family protein [Populus trichocarpa]
gi|550323214|gb|ERP52702.1| transferase family protein
[Populus trichocarpa]
Length = 460
Score = 322 bits (824), Expect(2) = e-103
Identities = 163/301 (54%), Positives = 215/301 (71%), Gaps = 3/301 (0%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M + +S+CFIKPKH E ++QP YL+P D+ + V Y+Q+G +FAKP T+ +
Sbjct: 1 MNPLTVTPISECFIKPKHIVEEARQPYYLAPWDLVMLSVHYIQKGLLFAKPP--KTENQQ 58
Query: 1333 N-QLQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASL 1157
++ +F+E LK+SLS TLVHFYPLAGRLAT K E PS + F VDCN PGARF +A++
Sbjct: 59 GFKIMDFLEDLKQSLSLTLVHFYPLAGRLATSKSENPPSYVVF-VDCNNSPGARFTYATV 117
Query: 1156 DLTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGD 977
DLT ++SPTY+P +++S FDH R LN+DGHT+SLLTIQVTELIDGIF+GCS+NH + D
Sbjct: 118 DLTTSDVLSPTYVPLVVQSFFDHDRALNHDGHTKSLLTIQVTELIDGIFIGCSVNHSIVD 177
Query: 976 GTSLWHFLASWSEIFKAEGKSIAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTITRD 797
G+S WHF WSEIF+ +G ++ISRPP+ RWFP G P +SLP T
Sbjct: 178 GSSFWHFFNMWSEIFQGKGDDVSISRPPVLNRWFPDG-HGPALSLP----------FTHH 226
Query: 796 EADTSP--ANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRCLLRAWNF 623
+ SP A ++KER+FHFSSES+ +LKAKAN E++T +ISSFQ+LSA +WRC+ RA N
Sbjct: 227 DQFLSPFEAPLLKERMFHFSSESIARLKAKANAESNTNKISSFQSLSALVWRCITRARNL 286
Query: 622 P 620
P
Sbjct: 287 P 287
Score = 84.7 bits (208), Expect(2) = e-103
Identities = 50/130 (38%), Positives = 73/130 (56%), Gaps = 13/130 (10%)
Frame = -3
Query: 594 SAIGGCGKLLENGLGWTAWLLHQAVV--NKEMSYEWINSRLQSLSLPRNTSVTKPGFIVQ 421
+ + G+LLE LGW AW LHQAVV + E + E++N L+S + + + P ++
Sbjct: 322 AGVSTVGELLEQNLGWAAWQLHQAVVSHSDEKAREFLNLWLKSRFIYQIGKLFDPHSVMM 381
Query: 420 TGSPRFDLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLE 274
SPRF+ + +FGLGK +A R+G A G ++ ICL P M++LE
Sbjct: 382 GSSPRFNKYGNEFGLGKALALRSGYAHKFSGKASAYPGHEGGGSIELEICLSPDEMSALE 441
Query: 273 SDKEFMETVS 244
SDKEFM+ VS
Sbjct: 442 SDKEFMDVVS 451
>ref|XP_006374906.1| anthranilate N-hydroxycinamoyl/bensoiltransferase-like family protein
[Populus trichocarpa] gi|550323215|gb|ERP52703.1|
anthranilate N-hydroxycinamoyl/bensoiltransferase-like
family protein [Populus trichocarpa]
Length = 455
Score = 320 bits (819), Expect(2) = e-103
Identities = 164/304 (53%), Positives = 212/304 (69%), Gaps = 6/304 (1%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M ++ +++CF+KPKH E +KQP YLSP D+ + Y+Q+G +FAKP E
Sbjct: 1 MNPTTVKHITECFLKPKHIVEEAKQPYYLSPWDLAMLSSHYIQKGLLFAKPPQ-----TE 55
Query: 1333 NQL----QEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVH 1166
NQL +F+E LK+SLS TLVHFYPLAGRLAT K E PS + F VDCN PGARF +
Sbjct: 56 NQLGFKIMDFLEDLKQSLSLTLVHFYPLAGRLATSKSENPPSYVVF-VDCNNSPGARFTY 114
Query: 1165 ASLDLTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHM 986
A++DLT ++SPTY+P +++S FDH R LN+DGHT+SLLTIQVTELIDGIF+GCS+NH
Sbjct: 115 ATVDLTASDVLSPTYVPLVVQSFFDHDRALNHDGHTKSLLTIQVTELIDGIFIGCSVNHS 174
Query: 985 LGDGTSLWHFLASWSEIFKAEGKSIAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTI 806
+ DG+S WHF WSEIF+ +G ++ISRPP+ RWFP G P +SLP
Sbjct: 175 IVDGSSFWHFFNMWSEIFQGKGDDVSISRPPVLNRWFPDG-HGPALSLP----------F 223
Query: 805 TRDEADTSP--ANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRCLLRA 632
T + P A ++KER+FHFSSES+ +LKAKAN E +T +ISSFQ+LSA +WRC+ RA
Sbjct: 224 THHDQFLRPFEAPLLKERMFHFSSESIARLKAKANAEFNTNKISSFQSLSALVWRCITRA 283
Query: 631 WNFP 620
N P
Sbjct: 284 RNLP 287
Score = 86.3 bits (212), Expect(2) = e-103
Identities = 50/130 (38%), Positives = 73/130 (56%), Gaps = 13/130 (10%)
Frame = -3
Query: 594 SAIGGCGKLLENGLGWTAWLLHQAVVN--KEMSYEWINSRLQSLSLPRNTSVTKPGFIVQ 421
+ + G++LE LGWTAW LHQAVV+ E + E++N L+S + + P ++
Sbjct: 322 AGVSTAGEVLEQNLGWTAWQLHQAVVSHGDEKAREFLNLWLKSPFINHIGKLFDPHSVMM 381
Query: 420 TGSPRFDLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLE 274
SPRF+ + +FGLGK +A R+G A G ++ ICL P MT++E
Sbjct: 382 GSSPRFNKYGNEFGLGKALALRSGYALKFSGKASAYPGHEGGGSIELEICLSPDEMTAIE 441
Query: 273 SDKEFMETVS 244
SDKEFM+ VS
Sbjct: 442 SDKEFMDVVS 451
>ref|XP_011029557.1| PREDICTED: uncharacterized acetyltransferase At3g50280-like [Populus
euphratica]
Length = 460
Score = 317 bits (811), Expect(2) = e-101
Identities = 161/301 (53%), Positives = 213/301 (70%), Gaps = 3/301 (0%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M A+ +S+CFIKPKH E ++QP YL+P D+ + V Y+Q+G +FAKP T+ +
Sbjct: 1 MNPLAVTPISECFIKPKHIVEEARQPYYLAPWDLVMLSVQYIQKGLLFAKPP--KTENQQ 58
Query: 1333 N-QLQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASL 1157
++ +F+E LK+SLS TLVHFYPLAGRLAT K E PS + F VDCN PGARF +A++
Sbjct: 59 GFKIMDFLEDLKQSLSLTLVHFYPLAGRLATSKSENPPSYVVF-VDCNNSPGARFTYATV 117
Query: 1156 DLTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGD 977
DLT ++SPTY+P +++S FDH R LN+DGHT+SLLTIQVTEL DGIF+GCS+NH + D
Sbjct: 118 DLTKSDVLSPTYVPLVVQSFFDHDRALNHDGHTKSLLTIQVTELTDGIFIGCSINHSIVD 177
Query: 976 GTSLWHFLASWSEIFKAEGKSIAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTITRD 797
G+S WHF WSEIF+ +G ++ISRPP+ RWFP G P ++LP T
Sbjct: 178 GSSFWHFFNMWSEIFQGKGDDVSISRPPVLNRWFPDG-HGPVLTLP----------FTHH 226
Query: 796 EADTSP--ANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRCLLRAWNF 623
+ SP A ++KER+FHFSSES+ + KAKAN E +T +ISSFQ+LSA +WRC+ RA N
Sbjct: 227 DQFLSPFEAPLLKERMFHFSSESIARPKAKANAEINTNKISSFQSLSALVWRCITRARNL 286
Query: 622 P 620
P
Sbjct: 287 P 287
Score = 83.2 bits (204), Expect(2) = e-101
Identities = 50/130 (38%), Positives = 72/130 (55%), Gaps = 13/130 (10%)
Frame = -3
Query: 594 SAIGGCGKLLENGLGWTAWLLHQAVV--NKEMSYEWINSRLQSLSLPRNTSVTKPGFIVQ 421
+ + G+LLE LGW AW LHQAVV + E + E+++ L+S + + + P ++
Sbjct: 322 AGVSTAGELLEQNLGWAAWQLHQAVVSHSDEKAREFLSLWLKSRFIYQIGKLFDPHSVMM 381
Query: 420 TGSPRFDLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLE 274
SPRF+ + +FGLGK +A R+G A G ++ ICL P MT+LE
Sbjct: 382 GSSPRFNKYGNEFGLGKALALRSGYAHKFSGKASAYPGHEGGGSIELEICLSPDEMTALE 441
Query: 273 SDKEFMETVS 244
SDKEFM VS
Sbjct: 442 SDKEFMGVVS 451
>ref|XP_012068314.1| PREDICTED: uncharacterized acetyltransferase At3g50280-like [Jatropha
curcas] gi|643735033|gb|KDP41703.1| hypothetical protein
JCGZ_16110 [Jatropha curcas]
Length = 450
Score = 321 bits (822), Expect(2) = e-101
Identities = 164/301 (54%), Positives = 211/301 (70%), Gaps = 3/301 (0%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M AI+ +S+CFIKP+H E ++QP YLSP D+ V V Y+Q+G +F KP D +
Sbjct: 1 MNPTAIQYISECFIKPQHVVEEAEQPYYLSPWDLAMVSVHYIQKGLLFPKPPREDFN--- 57
Query: 1333 NQLQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASLD 1154
+ +EKLK SL+ TLVHFYPLAGRLAT K ++ PS YVDC PGARFVHA++D
Sbjct: 58 --ILHLLEKLKHSLALTLVHFYPLAGRLATSKSQDPPS-YVIYVDCRNSPGARFVHATVD 114
Query: 1153 LTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGDG 974
+T+ I+SPTY+P +++S FDH+R LN+DGH SLLTIQVTEL+DGIF+GCS NH +GDG
Sbjct: 115 MTVSDILSPTYVPSVVQSFFDHHRALNHDGHVRSLLTIQVTELVDGIFIGCSFNHAIGDG 174
Query: 973 TSLWHFLASWSEIFKAEG-KSIAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTITRD 797
TS WHFL +WSEIF+AEG AISRPP+ + WFP G P ++LP T
Sbjct: 175 TSYWHFLNTWSEIFQAEGDDGAAISRPPVLKPWFPDG-YGPVINLP----------FTHH 223
Query: 796 EADTSP--ANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRCLLRAWNF 623
E P + V+KERIFHFS+ES+ KLK KAN E +T +ISSFQ+LSA +WRC+ R+ +
Sbjct: 224 EQFLCPFESPVLKERIFHFSAESIAKLKEKANAEYNTNKISSFQSLSAFMWRCITRSRHL 283
Query: 622 P 620
P
Sbjct: 284 P 284
Score = 79.0 bits (193), Expect(2) = e-101
Identities = 48/130 (36%), Positives = 67/130 (51%), Gaps = 13/130 (10%)
Frame = -3
Query: 594 SAIGGCGKLLENGLGWTAWLLHQAVVNK--EMSYEWINSRLQSLSLPRNTSVTKPGFIVQ 421
+A G+LL+ LGW AW+LHQ+VVN+ E E I + +S + P ++
Sbjct: 318 AASSTAGELLQQNLGWAAWMLHQSVVNQTSEKIQERIEAWKKSPFIYHIGRSFDPFSVMM 377
Query: 420 TGSPRFDLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLE 274
SPRF+ + FGLGK + R+G A G D+ +CLPP +M +LE
Sbjct: 378 GSSPRFNKYGCQFGLGKALTLRSGYAHKFSGKVSAYPGREGGGSVDLEVCLPPDSMAALE 437
Query: 273 SDKEFMETVS 244
SDKEF VS
Sbjct: 438 SDKEFFAVVS 447
>emb|CDP06257.1| unnamed protein product [Coffea canephora]
Length = 449
Score = 311 bits (796), Expect(2) = e-101
Identities = 156/293 (53%), Positives = 209/293 (71%)
Frame = -2
Query: 1498 IRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDENQLQE 1319
I VS+C ++PK+ P+ S+Q +Y +P D+ + Y Q+G +FAKP FD++G ++++
Sbjct: 7 IHYVSECLVQPKYVPQDSEQRIYFAPWDLSLASIHYNQKGLLFAKPPAFDSEG---KMKD 63
Query: 1318 FVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASLDLTIDA 1139
F++KLKESLS TLVHFYPLAGRLAT K +ENP + YVDC PGA+FVHAS+DLTID
Sbjct: 64 FLQKLKESLSLTLVHFYPLAGRLATLK-QENPPIYSIYVDCTNLPGAKFVHASVDLTIDD 122
Query: 1138 IISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGDGTSLWH 959
++P Y+P+I+ S FDH +N+DGH+ SL++IQVTEL DGIF+G S NH+L DGTS WH
Sbjct: 123 FLTPIYMPKIVHSFFDHVGAVNHDGHSMSLMSIQVTELKDGIFIGWSTNHLLVDGTSFWH 182
Query: 958 FLASWSEIFKAEGKSIAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTITRDEADTSP 779
F+ +WSE+F A+G+ ISRPPI +RWFP G +P + LP I+R +
Sbjct: 183 FINTWSEVFNAKGQIPTISRPPILKRWFPEGHRSPVIGLPFTHH---DEFISRFQTPE-- 237
Query: 778 ANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRCLLRAWNFP 620
+ ER FHFS+ES+ KLKAKAN E ++T IS+FQALSA LWRC+ RA N P
Sbjct: 238 ---LLERYFHFSAESLAKLKAKANAECNSTTISTFQALSALLWRCITRARNMP 287
Score = 89.0 bits (219), Expect(2) = e-101
Identities = 52/126 (41%), Positives = 73/126 (57%), Gaps = 14/126 (11%)
Frame = -3
Query: 579 CGKLLENGLGWTAWLLHQAVVNK--EMSYEWINSRLQSLSLPRNTSVTKPGFIVQTG-SP 409
CG+LL NGLGW AW LH+ V+N+ EW+ S ++S P + + +Q G SP
Sbjct: 326 CGELLTNGLGWAAWKLHELVINQTDRSIREWVESWVKS---PFIVWIGRFSNRIQIGSSP 382
Query: 408 RFDLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLESDKE 262
RF+++ +FGLGK +A R+G A G D+ +CLPP +M+ LESD+E
Sbjct: 383 RFNVYGSEFGLGKALAVRSGFANKPDGKITLFPGREGGGSMDVEVCLPPQSMSFLESDQE 442
Query: 261 FMETVS 244
FME VS
Sbjct: 443 FMENVS 448
>emb|CDP06258.1| unnamed protein product [Coffea canephora]
Length = 453
Score = 312 bits (800), Expect(2) = e-100
Identities = 159/293 (54%), Positives = 210/293 (71%)
Frame = -2
Query: 1498 IRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDENQLQE 1319
I VS+C ++PKH P+ S+Q +YL+P D+ + Y Q G +FAKP FD++G ++++
Sbjct: 11 IHYVSECLVQPKHIPQDSEQRIYLAPWDLRLASLHYNQNGLLFAKPPAFDSEG---KMKD 67
Query: 1318 FVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASLDLTIDA 1139
+++KLKESLS TLVHFYPLAGRLAT K +ENP + YVDC PGA+FVHAS+DLTID
Sbjct: 68 YLQKLKESLSLTLVHFYPLAGRLATLK-QENPPIYSIYVDCTNLPGAKFVHASVDLTIDD 126
Query: 1138 IISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGDGTSLWH 959
I++P Y+P+I+ S FDH +N+DGH+ SL++IQVTEL DGIF+G S NH+L DGTS WH
Sbjct: 127 ILTPIYMPKIVYSFFDHVGAVNHDGHSMSLISIQVTELKDGIFIGWSTNHLLVDGTSFWH 186
Query: 958 FLASWSEIFKAEGKSIAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTITRDEADTSP 779
F+ +WSE+F A+G+ ISRPPI +RWFP G +P SLP I+R +
Sbjct: 187 FINTWSEVFNAKGQISTISRPPILKRWFPEGHRSPVFSLPFAHH---DEFISRFQTPE-- 241
Query: 778 ANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRCLLRAWNFP 620
+ ER FHFS+ES+ KLKAKAN E ++T IS+FQALSA LWRC+ RA N P
Sbjct: 242 ---LLERYFHFSAESLAKLKAKANAECNSTNISTFQALSALLWRCITRARNMP 291
Score = 83.2 bits (204), Expect(2) = e-100
Identities = 51/126 (40%), Positives = 69/126 (54%), Gaps = 14/126 (11%)
Frame = -3
Query: 579 CGKLLENGLGWTAWLLHQAVVNK--EMSYEWINSRLQSLSLPRNTSVTKPGFIVQTG-SP 409
CG+LL NGLGW AW LH V+N+ EW+ S ++S P + + +Q G SP
Sbjct: 330 CGELLTNGLGWAAWKLHDLVINQTDRSIREWVESWVKS---PFIVWIGRFSNRIQIGNSP 386
Query: 408 RFDLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLESDKE 262
RF+++ +FGLGK +A R+G A G D+ CLP M+ LESD+E
Sbjct: 387 RFNIYGNEFGLGKALAVRSGFANKLDGKITLFPGREGGGSMDVEACLPQQTMSFLESDQE 446
Query: 261 FMETVS 244
FME VS
Sbjct: 447 FMENVS 452
>ref|XP_006473294.1| PREDICTED: uncharacterized acetyltransferase At3g50280-like [Citrus
sinensis]
Length = 455
Score = 305 bits (782), Expect(2) = 2e-99
Identities = 157/302 (51%), Positives = 216/302 (71%), Gaps = 4/302 (1%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M SPA++ +S+CF++P++ E KQP YL+P D+ + V Y+Q+G +F KP+ D+
Sbjct: 1 MNSPAVQYMSECFVQPEYFSEEMKQPCYLTPWDLVMLSVQYIQKGLLFRKPAIPPASVDQ 60
Query: 1333 -NQLQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASL 1157
+ ++ +++LK SLS TLVHFYPLAGRL T++ + PS + F VDCN PGA+F+HASL
Sbjct: 61 VDFIKTLLDRLKHSLSLTLVHFYPLAGRLVTRQTDSPPSYVVF-VDCNNSPGAKFIHASL 119
Query: 1156 DLTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGD 977
D+ I I+SPTY+P +++S FDH R LN+DGH+ LL+IQVTEL+DGIF+GCSMNH+LGD
Sbjct: 120 DMAISDILSPTYVPLVVQSFFDHDRALNHDGHSRPLLSIQVTELVDGIFIGCSMNHVLGD 179
Query: 976 GTSLWHFLASWSEIFKAEGKSIAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTITRD 797
GTS W+F ++SEIF+A+GK +ISRPP+ +RWFP GR+ P ++LP RD
Sbjct: 180 GTSFWNFFNAFSEIFQAQGKMASISRPPVIKRWFPDGRD-PIINLPFTH---------RD 229
Query: 796 E-ADTSPANVIKERIFHFSSESVRKLKAKANFEA--DTTEISSFQALSAHLWRCLLRAWN 626
E A + ERIFHF+ ES+ +LKAKAN EA +T +ISSFQALSA +WR + R N
Sbjct: 230 EFIRRYEAPQLLERIFHFTGESIARLKAKANAEAGNNTNKISSFQALSALVWRSITRIRN 289
Query: 625 FP 620
P
Sbjct: 290 LP 291
Score = 87.0 bits (214), Expect(2) = 2e-99
Identities = 50/124 (40%), Positives = 71/124 (57%), Gaps = 13/124 (10%)
Frame = -3
Query: 576 GKLLENGLGWTAWLLHQAVVNK--EMSYEWINSRLQSLSLPRNTSVTKPGFIVQTGSPRF 403
G+LL + LGW AW LHQ VVN+ + EW+++ +S + + P I+ SPRF
Sbjct: 331 GELLGHDLGWAAWKLHQTVVNQTDKAVREWLDAWWKSPFMYHLDRLFDPFSIMMGSSPRF 390
Query: 402 DLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLESDKEFM 256
+ + +FGLG+ VA R+G A G D+ ICLPPH+MT+LE+D+EFM
Sbjct: 391 NKYGNEFGLGQAVALRSGYAHKFDGKVSAYPGHEGGGSIDLEICLPPHSMTALEADEEFM 450
Query: 255 ETVS 244
E S
Sbjct: 451 EATS 454
>gb|KDO84111.1| hypothetical protein CISIN_1g044173mg [Citrus sinensis]
Length = 455
Score = 305 bits (781), Expect(2) = 3e-99
Identities = 157/302 (51%), Positives = 216/302 (71%), Gaps = 4/302 (1%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M SPA++ +S+CF++P++ E KQP YL+P D+ + V Y+Q+G +F KP+ D+
Sbjct: 1 MNSPAVQYMSECFVQPEYFSEEMKQPCYLTPWDLVMLSVQYIQKGLLFRKPAIPPASVDQ 60
Query: 1333 -NQLQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASL 1157
+ ++ +++LK SLS TLVHFYPLAGRL T++ + PS + F VDCN PGA+F+HASL
Sbjct: 61 VDFIKTLLDRLKHSLSLTLVHFYPLAGRLVTRQTDSPPSYVVF-VDCNNSPGAKFIHASL 119
Query: 1156 DLTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGD 977
D+ I I+SPTY+P +++S FDH R LN+DGH+ LL+IQVTEL+DGIF+GCSMNH+LGD
Sbjct: 120 DMAISDILSPTYVPLVVQSFFDHDRALNHDGHSRPLLSIQVTELVDGIFIGCSMNHVLGD 179
Query: 976 GTSLWHFLASWSEIFKAEGKSIAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTITRD 797
GTS W+F ++SEIF+A+GK +ISRPP+ +RWFP GR+ P ++LP RD
Sbjct: 180 GTSFWNFFNAFSEIFQAQGKMDSISRPPVVKRWFPDGRD-PIINLPFTH---------RD 229
Query: 796 E-ADTSPANVIKERIFHFSSESVRKLKAKANFEA--DTTEISSFQALSAHLWRCLLRAWN 626
E A + ERIFHF+ ES+ +LKAKAN EA +T +ISSFQALSA +WR + R N
Sbjct: 230 EFIRRYEAPQLLERIFHFTGESIARLKAKANAEAGNNTNKISSFQALSALVWRSITRTRN 289
Query: 625 FP 620
P
Sbjct: 290 LP 291
Score = 87.0 bits (214), Expect(2) = 3e-99
Identities = 50/124 (40%), Positives = 71/124 (57%), Gaps = 13/124 (10%)
Frame = -3
Query: 576 GKLLENGLGWTAWLLHQAVVNK--EMSYEWINSRLQSLSLPRNTSVTKPGFIVQTGSPRF 403
G+LL + LGW AW LHQ VVN+ + EW+++ +S + + P I+ SPRF
Sbjct: 331 GELLGHDLGWAAWKLHQTVVNQTDKAVREWLDAWWKSPFMYHLDRLFDPFSIMMGSSPRF 390
Query: 402 DLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLESDKEFM 256
+ + +FGLG+ VA R+G A G D+ ICLPPH+MT+LE+D+EFM
Sbjct: 391 NKYGNEFGLGQAVALRSGYAHKFDGKVSAYPGHEGGGSIDLEICLPPHSMTALEADEEFM 450
Query: 255 ETVS 244
E S
Sbjct: 451 EVTS 454
>ref|XP_012066805.1| PREDICTED: uncharacterized acetyltransferase At3g50280-like isoform
X1 [Jatropha curcas] gi|643735959|gb|KDP42394.1|
hypothetical protein JCGZ_02451 [Jatropha curcas]
Length = 448
Score = 315 bits (806), Expect(2) = 4e-99
Identities = 160/301 (53%), Positives = 213/301 (70%), Gaps = 3/301 (0%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M A++ +S+ FIKP+H E +K P YLSP D+ V Y+QRG +FAKP T ++
Sbjct: 1 MNPTAVQHISESFIKPQHMTEETKHPYYLSPCDLGMVSACYIQRGLLFAKP----TPQED 56
Query: 1333 NQLQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASLD 1154
++ + +EKLK SL+ TLVHFYPLAGRLAT K E+ PS YVDC+ PGARFVHA+++
Sbjct: 57 FKIFDLLEKLKHSLALTLVHFYPLAGRLATSKSEDPPS-YVIYVDCSNSPGARFVHATVE 115
Query: 1153 LTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGDG 974
+T+ I+SPTY+P +++S FDH+R LN++GH SLLTIQVTELIDGIF+GCS NH++GDG
Sbjct: 116 MTVSDILSPTYVPSVVQSFFDHHRALNHEGHVRSLLTIQVTELIDGIFIGCSFNHVIGDG 175
Query: 973 TSLWHFLASWSEIFKAE-GKSIAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTITRD 797
TS WHFL +WSEIF+A+ + IS+PP+ +RWFP P ++LP T
Sbjct: 176 TSYWHFLNTWSEIFQAQRDDHVPISQPPVLKRWFPDNC-GPIINLP----------FTHH 224
Query: 796 EADTSP--ANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRCLLRAWNF 623
E P A ++KERIFHFS+ES+ KLK KAN E +T +ISSFQ+LSA +WRC+ RA +
Sbjct: 225 EQFLVPFEAPLLKERIFHFSAESIAKLKVKANTEYNTDKISSFQSLSAFMWRCITRARHI 284
Query: 622 P 620
P
Sbjct: 285 P 285
Score = 77.0 bits (188), Expect(2) = 4e-99
Identities = 50/126 (39%), Positives = 69/126 (54%), Gaps = 13/126 (10%)
Frame = -3
Query: 582 GCGKLLENGLGWTAWLLHQAVVNK--EMSYEWINSRLQSLSLPRNTSVTKPGFIVQTGSP 409
GC +LLE LGW AWLLHQAV+N+ + + I+S ++S + +V SP
Sbjct: 324 GC-ELLEQNLGWAAWLLHQAVINQTDKKFRDRIDSWIKSPIIYHLGQFFGLYGVVFGSSP 382
Query: 408 RFDLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLESDKE 262
RF + +FGLGK ++ R+G A G D+ +CL P +MT+LESDKE
Sbjct: 383 RFSKYGCEFGLGKALSLRSGYANKFCGKVSAYPGREGGGSVDLEVCLLPDSMTALESDKE 442
Query: 261 FMETVS 244
FM VS
Sbjct: 443 FMAAVS 448
>ref|XP_012452172.1| PREDICTED: uncharacterized acetyltransferase At3g50280-like
[Gossypium raimondii] gi|763796927|gb|KJB63882.1|
hypothetical protein B456_010G021700 [Gossypium
raimondii]
Length = 455
Score = 307 bits (786), Expect(2) = 1e-98
Identities = 152/299 (50%), Positives = 213/299 (71%), Gaps = 1/299 (0%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M ++R +S+C++ P+H + SKQP YL+ D+ + V Y+Q+G ++AKP G + +E
Sbjct: 1 MDPSSVRIISECYVTPQHVSDQSKQPYYLTTWDLAMLSVQYIQKGLLYAKPEG---NCEE 57
Query: 1333 NQLQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASLD 1154
N + + +LK+SLS LVHFYPLAGRLAT K EENP +VDCN PGA+F+HA++D
Sbjct: 58 NLINNVLGRLKQSLSIALVHFYPLAGRLAT-KIEENPKSHFVFVDCNNSPGAKFIHAAVD 116
Query: 1153 LTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGDG 974
L++ I+SPTY+P +++S FDH R +NYDGHT LL+IQVTEL+DG+F+GCSMNH +GDG
Sbjct: 117 LSVSDIVSPTYVPLVVQSFFDHDRAINYDGHTRPLLSIQVTELVDGVFIGCSMNHAIGDG 176
Query: 973 TSLWHFLASWSEIFKAEG-KSIAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTITRD 797
TS WHF + SEIF+A+G ++ ISRPP+ E+WFP G P ++LP I+R
Sbjct: 177 TSFWHFFNTLSEIFQAQGDTNLKISRPPVLEKWFPEG-HGPLLNLPFTNQ---DELISRF 232
Query: 796 EADTSPANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRCLLRAWNFP 620
EA + ERIFHFS++S+ KLK +AN E++TT+ISSFQ+LSA +WR + +A FP
Sbjct: 233 EAPE-----LLERIFHFSAKSIAKLKERANTESNTTKISSFQSLSAFVWRSITKARRFP 286
Score = 82.8 bits (203), Expect(2) = 1e-98
Identities = 49/129 (37%), Positives = 69/129 (53%), Gaps = 13/129 (10%)
Frame = -3
Query: 591 AIGGCGKLLENGLGWTAWLLHQAVVN--KEMSYEWINSRLQSLSLPRNTSVTKPGFIVQT 418
A+ G+LL +GLGW AW LHQAVVN + ++N L S + + + P ++
Sbjct: 321 AVTTAGELLNHGLGWAAWKLHQAVVNHTDKQVRGFVNGWLDSPFIYQIAQLFDPQSVMMG 380
Query: 417 GSPRFDLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLES 271
SPRF+ + +FGLGK + R+G A G D+ ICLPP +M +LE
Sbjct: 381 SSPRFNKYGNEFGLGKALTLRSGYAHKFDGKVSAYPGHEGGGSIDLEICLPPSSMKALEL 440
Query: 270 DKEFMETVS 244
D+EFM VS
Sbjct: 441 DEEFMSVVS 449
>ref|XP_012066803.1| PREDICTED: uncharacterized acetyltransferase At3g50280-like [Jatropha
curcas] gi|643735957|gb|KDP42392.1| hypothetical protein
JCGZ_02449 [Jatropha curcas]
Length = 448
Score = 318 bits (814), Expect(2) = 1e-98
Identities = 160/299 (53%), Positives = 212/299 (70%), Gaps = 1/299 (0%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M S A++ +S+ FIKP+H E SK P YLSP D+ V Y+Q+G +FAKP T ++
Sbjct: 1 MNSTAVQHISESFIKPQHMTEESKHPYYLSPCDLAMVSAYYIQKGLLFAKP----TPQED 56
Query: 1333 NQLQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASLD 1154
++ + +EKLK SL+ TLVHFYPLAGRLAT K E+ PS YVDC+ PGARFVHA++D
Sbjct: 57 FKIFDLLEKLKHSLALTLVHFYPLAGRLATSKSEDPPS-YVIYVDCSNSPGARFVHATVD 115
Query: 1153 LTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGDG 974
+T+ I+SPTY+P +++S FDH+R LN++GH SLLTIQVTELIDGIF+GCS NH++GDG
Sbjct: 116 MTVSDILSPTYVPSVIQSFFDHHRALNHEGHVRSLLTIQVTELIDGIFIGCSFNHVIGDG 175
Query: 973 TSLWHFLASWSEIFKAE-GKSIAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTITRD 797
TS WHFL +WSEIF+A+ + IS+PP+ +RWFP P ++LP V
Sbjct: 176 TSYWHFLNTWSEIFQAQRDDHVPISQPPVLKRWFPDNC-GPIINLPFNHHEQFLVPF--- 231
Query: 796 EADTSPANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRCLLRAWNFP 620
A ++KERIFHFS+ES+ KLK KAN E +T +ISSFQ+LSA +WRC+ RA + P
Sbjct: 232 -----EAPLLKERIFHFSAESIAKLKVKANTEYNTDKISSFQSLSAFMWRCITRARHIP 285
Score = 72.0 bits (175), Expect(2) = 1e-98
Identities = 48/126 (38%), Positives = 67/126 (53%), Gaps = 13/126 (10%)
Frame = -3
Query: 582 GCGKLLENGLGWTAWLLHQAVVNK--EMSYEWINSRLQSLSLPRNTSVTKPGFIVQTGSP 409
GC +LLE LGW AWLLHQAV+N+ + + I+S ++S + + SP
Sbjct: 324 GC-ELLEQNLGWAAWLLHQAVINQTDKKFRDRIDSWIKSPIIYHLGQFFGLYGVHFGSSP 382
Query: 408 RFDLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLESDKE 262
RF+ + + GLGK + R+G A G D+ +CL P +MT+LESDKE
Sbjct: 383 RFNKYGCELGLGKALTLRSGYAHKFCGKVSAYPGREGGGSVDLEVCLLPDSMTALESDKE 442
Query: 261 FMETVS 244
FM VS
Sbjct: 443 FMAAVS 448
>ref|XP_007224078.1| hypothetical protein PRUPE_ppa015472mg [Prunus persica]
gi|462421014|gb|EMJ25277.1| hypothetical protein
PRUPE_ppa015472mg [Prunus persica]
Length = 465
Score = 308 bits (788), Expect(2) = 2e-97
Identities = 161/308 (52%), Positives = 212/308 (68%), Gaps = 10/308 (3%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M S +++ +S+CF++P+H + SKQPLYL+P D+ + Y+Q+G +FAKP D +
Sbjct: 1 MSSQSVKHISECFVQPQHASDESKQPLYLAPTDLPMLSAHYIQKGLLFAKPPEAMNDHKK 60
Query: 1333 NQ-LQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASL 1157
+ +EKLK SLS LVHFYPLAGRLAT KKEENP Y+DCN PGA+F++A++
Sbjct: 61 ADFINALLEKLKHSLSLALVHFYPLAGRLAT-KKEENPPLYLVYIDCNNSPGAKFIYATV 119
Query: 1156 DLTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGD 977
DL+I I+SPT +P +++S FDH R +N+DGHT SLLT QVTEL+DGIF+G SMNH L D
Sbjct: 120 DLSISDILSPTDVPLVVQSFFDHDRAVNHDGHTMSLLTAQVTELVDGIFIGLSMNHCLAD 179
Query: 976 GTSLWHFLASWSEIFKAEGKSIA---------ISRPPIHERWFPHGRENPFVSLPVIRDL 824
GTS WHF +WSEIF+A+ ++ A ISRPP+ +RWFP G + P +SLP
Sbjct: 180 GTSYWHFFNTWSEIFQAQTQTHASQNNNIIPYISRPPVLKRWFPEGHD-PIISLPYTHP- 237
Query: 823 DGQVTITRDEADTSPANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRC 644
I R EA ++ER+FHFSSE++ KLKAKAN EA TT+ISSFQ+LSA LWRC
Sbjct: 238 --DEFIGRFEAPK-----LRERMFHFSSEALAKLKAKANAEAKTTKISSFQSLSALLWRC 290
Query: 643 LLRAWNFP 620
+ R P
Sbjct: 291 ITRVRRLP 298
Score = 78.2 bits (191), Expect(2) = 2e-97
Identities = 47/129 (36%), Positives = 70/129 (54%), Gaps = 12/129 (9%)
Frame = -3
Query: 594 SAIGGCGKLLENGLGWTAWLLHQAVVN--KEMSYEWINSRLQSLSLPRNTSVTKPGFIVQ 421
S + G+LLE+GLGW AW LH+AVVN + E+I++ LQS + + P ++
Sbjct: 333 SEVVTAGELLEHGLGWAAWKLHEAVVNLDDKSIREFIDAWLQSRMVYQIGQFFDPHSVMM 392
Query: 420 TGSPRFDLHAIDFGLGKPVATRTGNAG----------XXXXXXXDILICLPPHAMTSLES 271
SP F+++ +FG+GK +A R+G A D+ +CL P AM ++E
Sbjct: 393 GSSPWFNMYGNEFGMGKALALRSGYANKFSGKVSSYPGGERGSIDLEVCLVPDAMGAIEC 452
Query: 270 DKEFMETVS 244
D EFME S
Sbjct: 453 DHEFMEAAS 461
>ref|XP_007221613.1| hypothetical protein PRUPE_ppb015328mg [Prunus persica]
gi|462418549|gb|EMJ22812.1| hypothetical protein
PRUPE_ppb015328mg [Prunus persica]
Length = 453
Score = 303 bits (775), Expect(2) = 3e-97
Identities = 158/304 (51%), Positives = 208/304 (68%), Gaps = 6/304 (1%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M SP+++ +S+CF++P H E SKQP YL+P D+ + V Y+Q+G +F+KP G E
Sbjct: 1 MNSPSVKYISECFVQPHHASEESKQPFYLTPGDLAMLSVHYIQKGLLFSKPPEAKL-GQE 59
Query: 1333 NQLQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASLD 1154
+ +E+LK SLS LVHFYPLAGR T+K E+ P L YVDCN PGA+F++A+LD
Sbjct: 60 DFTNSLLERLKHSLSIALVHFYPLAGRFVTEKNEDPPLYLV-YVDCNNSPGAKFINATLD 118
Query: 1153 LTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGDG 974
++I I+SPT P +++S FDH R +N+DGHT SLLTIQVTEL+DGIF+G SMNH LGDG
Sbjct: 119 MSISDILSPTDAPLVVQSFFDHDRAVNHDGHTMSLLTIQVTELVDGIFIGLSMNHCLGDG 178
Query: 973 TSLWHFLASWSEIFKAEGKS------IAISRPPIHERWFPHGRENPFVSLPVIRDLDGQV 812
TS WHF WSEIF+A+ + ISRPP+ +RWFP G P ++LP
Sbjct: 179 TSYWHFFNIWSEIFQAQAQGDHNNFVPKISRPPVLKRWFPDG-HGPIINLPF---KHSDE 234
Query: 811 TITRDEADTSPANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRCLLRA 632
I R EA ++ER+FHFSSES+ KLKAKAN E++TT+ISSFQ+LSA +WR + RA
Sbjct: 235 FIGRYEAPK-----LRERMFHFSSESIAKLKAKANAESNTTKISSFQSLSALMWRSITRA 289
Query: 631 WNFP 620
P
Sbjct: 290 RGLP 293
Score = 82.8 bits (203), Expect(2) = 3e-97
Identities = 50/129 (38%), Positives = 72/129 (55%), Gaps = 12/129 (9%)
Frame = -3
Query: 594 SAIGGCGKLLENGLGWTAWLLHQAVV--NKEMSYEWINSRLQSLSLPRNTSVTKPGFIVQ 421
S I G+LLE+GLGW AW LH+AVV N ++ E ++ L+S ++ S P ++
Sbjct: 322 SEIVPAGELLEHGLGWAAWKLHEAVVNHNDKLIREHVDGWLKSPAV-YQLSFFDPFSVMM 380
Query: 420 TGSPRFDLHAIDFGLGKPVATRTGNAG----------XXXXXXXDILICLPPHAMTSLES 271
SPRF+++ FG+GK +A R+G A D+ +CLPP AM++LE
Sbjct: 381 GSSPRFNMYGNQFGMGKALAPRSGYANKFSGKVSSYEGREEGSIDLEVCLPPDAMSALEC 440
Query: 270 DKEFMETVS 244
D EFME S
Sbjct: 441 DSEFMEAAS 449
>ref|XP_002510186.1| Anthranilate N-benzoyltransferase protein, putative [Ricinus
communis] gi|223550887|gb|EEF52373.1| Anthranilate
N-benzoyltransferase protein, putative [Ricinus communis]
Length = 452
Score = 300 bits (769), Expect(2) = 3e-97
Identities = 150/298 (50%), Positives = 204/298 (68%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M +++++S+CFIKP + P+ + QP YL+P D+ + V Y+Q+G +FAKP D
Sbjct: 1 MNPTSVQQISECFIKPPNLPKEANQPYYLTPWDLAMLSVHYIQKGLLFAKPPQEALD--- 57
Query: 1333 NQLQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASLD 1154
+ ++KLK SL+ TLVHFYPLAGRLAT K E+ PS + YVDC PGAR VHA+++
Sbjct: 58 --IMYLLDKLKHSLALTLVHFYPLAGRLATSKTEDPPSYVV-YVDCTNSPGARLVHAAVN 114
Query: 1153 LTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGDG 974
LT+ ++SPTY+P + +S FDH+R LN+DGH SLLTIQVT+L+DG+F+GCSMNH +GDG
Sbjct: 115 LTVSDVLSPTYVPLVAQSFFDHHRALNHDGHFRSLLTIQVTDLVDGVFIGCSMNHAIGDG 174
Query: 973 TSLWHFLASWSEIFKAEGKSIAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTITRDE 794
+S W FL S+SEIF+A+G I+ PP +RWFP G P V+LP T
Sbjct: 175 SSFWQFLNSFSEIFQAQGSEFQIAHPPDLKRWFPDG-YGPIVNLP--------FTHHEQF 225
Query: 793 ADTSPANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRCLLRAWNFP 620
A +ER+FHFSSES+ KLKA+AN E +T +ISSFQ+LSA +WRC++R P
Sbjct: 226 LGHFEAPPFRERVFHFSSESIAKLKARANSEYNTNKISSFQSLSAFMWRCIMRTRKLP 283
Score = 85.1 bits (209), Expect(2) = 3e-97
Identities = 50/124 (40%), Positives = 70/124 (56%), Gaps = 13/124 (10%)
Frame = -3
Query: 576 GKLLENGLGWTAWLLHQAVVNK--EMSYEWINSRLQSLSLPRNTSVTKPGFIVQTGSPRF 403
G+LLEN LGW AW HQAVVN+ E E + S L+S + + + P ++ SPRF
Sbjct: 323 GELLENNLGWAAWRTHQAVVNQSDEKIRESLESWLKSPIIYQIGQLFDPFSVMMGSSPRF 382
Query: 402 DLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLESDKEFM 256
+ + +FGLGK +A +G A G D+ +CLPP+ MT+LE D+EFM
Sbjct: 383 NKYGSEFGLGKALALLSGYAHKFSGKVSAYPGREGGGSVDLEVCLPPNLMTALEDDQEFM 442
Query: 255 ETVS 244
+ VS
Sbjct: 443 DAVS 446
>ref|XP_008220248.1| PREDICTED: uncharacterized acetyltransferase At3g50280-like isoform
X1 [Prunus mume] gi|645226884|ref|XP_008220249.1|
PREDICTED: uncharacterized acetyltransferase
At3g50280-like isoform X1 [Prunus mume]
Length = 459
Score = 303 bits (775), Expect(2) = 6e-97
Identities = 157/304 (51%), Positives = 208/304 (68%), Gaps = 6/304 (1%)
Frame = -2
Query: 1513 MGSPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDE 1334
M SP+++ +S+CF++P H E SKQP YL+P D+ + V Y+Q+G +F+KP G E
Sbjct: 1 MNSPSVKYISECFVQPHHASEESKQPFYLTPGDLAMLSVHYIQKGLLFSKPPEAKL-GQE 59
Query: 1333 NQLQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASLD 1154
+ +E+LK SLS LVHFYPLAGR T+K E+ P L YVDCN PGA+F++A+LD
Sbjct: 60 DFTNSLLERLKHSLSIALVHFYPLAGRFVTEKNEDPPLYLV-YVDCNNSPGAKFIYATLD 118
Query: 1153 LTIDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGDG 974
++I I+SPT +P +++S FDH R +N+DGHT SLLTIQVTEL+DGIF+G SMNH LGDG
Sbjct: 119 MSISDILSPTDVPLVVQSFFDHDRAVNHDGHTMSLLTIQVTELVDGIFIGLSMNHCLGDG 178
Query: 973 TSLWHFLASWSEIFKAEGKS------IAISRPPIHERWFPHGRENPFVSLPVIRDLDGQV 812
TS WHF WSEIF+A+ + ISRPP+ +RWFP G P ++LP
Sbjct: 179 TSYWHFFNIWSEIFQAQAQGDHNNFVPNISRPPVLKRWFPDG-HGPIINLPF---KHSDE 234
Query: 811 TITRDEADTSPANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRCLLRA 632
I R EA ++ER+FHFSSES+ KLKAKAN E++TT+ISSFQ+LSA +WR + R
Sbjct: 235 FIGRYEAPK-----LRERMFHFSSESIAKLKAKANAESNTTKISSFQSLSALMWRSITRV 289
Query: 631 WNFP 620
P
Sbjct: 290 RGLP 293
Score = 81.6 bits (200), Expect(2) = 6e-97
Identities = 50/130 (38%), Positives = 70/130 (53%), Gaps = 13/130 (10%)
Frame = -3
Query: 594 SAIGGCGKLLENGLGWTAWLLHQAVVNKEMSYEWINSRLQS-LSLPR--NTSVTKPGFIV 424
S I G+LL++GLGW AW LH+AVVN + +WI + L+ P S P ++
Sbjct: 328 SEIVPAGELLDHGLGWAAWKLHEAVVNH--NNKWIREHVDGWLNSPAVYQVSFFDPLSVM 385
Query: 423 QTGSPRFDLHAIDFGLGKPVATRTGNA----------GXXXXXXXDILICLPPHAMTSLE 274
SPRF+++ FG+GK +A R+G A D+ +CLPP AM++LE
Sbjct: 386 MGSSPRFNMYGNQFGMGKALALRSGYAHKFSGKVSSYEGREEGSIDLEVCLPPDAMSALE 445
Query: 273 SDKEFMETVS 244
D EFME S
Sbjct: 446 CDTEFMEAAS 455
>ref|XP_010110810.1| putative acetyltransferase [Morus notabilis]
gi|587941614|gb|EXC28182.1| putative acetyltransferase
[Morus notabilis]
Length = 454
Score = 305 bits (780), Expect(2) = 8e-97
Identities = 155/297 (52%), Positives = 210/297 (70%), Gaps = 1/297 (0%)
Frame = -2
Query: 1507 SPAIRRVSQCFIKPKHTPEASKQPLYLSPPDVCHVFVPYMQRGFIFAKPSGFDTDGDENQ 1328
+P + +S+CFIKP +TPE S+ PLYL+P D+ + V Y+Q+G +FAKP T+ DEN
Sbjct: 4 TPNVNHISECFIKPHYTPEDSENPLYLTPWDLAMLSVHYIQKGLLFAKPH---TNKDENF 60
Query: 1327 LQEFVEKLKESLSHTLVHFYPLAGRLATQKKEENPSDLAFYVDCNKGPGARFVHASLDLT 1148
+ +++LK+SLS LVHFYPLAGRL T K E NP + +VDCN GPGA+F++A+LD+T
Sbjct: 61 MGSLLDRLKQSLSVALVHFYPLAGRLKTVKTE-NPHSYSVHVDCNDGPGAKFIYATLDMT 119
Query: 1147 IDAIISPTYIPQILRSLFDHYRLLNYDGHTESLLTIQVTELIDGIFMGCSMNHMLGDGTS 968
+ I+SP +P I++ FDH R +N+DGHT SL+TIQVTEL+DG+F+GCSMNH++ DG S
Sbjct: 120 VSDILSPVDVPPIVQLFFDHDRAVNHDGHTTSLVTIQVTELVDGVFIGCSMNHVIADGAS 179
Query: 967 LWHFLASWSEIFKA-EGKSIAISRPPIHERWFPHGRENPFVSLPVIRDLDGQVTITRDEA 791
W+F WSEIF+A + K+ ISRPPI +RWFP G P +SLP I R EA
Sbjct: 180 FWNFWNMWSEIFRAKDNKNKQISRPPIFKRWFPEG-HGPILSLPYKHP---DEFIGRFEA 235
Query: 790 DTSPANVIKERIFHFSSESVRKLKAKANFEADTTEISSFQALSAHLWRCLLRAWNFP 620
++ER+FHFS ES+ KLKA+AN E +T +ISSFQ+LSA +WR ++R N P
Sbjct: 236 PK-----LRERVFHFSVESIAKLKARANLEYNTRKISSFQSLSALVWRSIIRVRNLP 287
Score = 79.3 bits (194), Expect(2) = 8e-97
Identities = 46/121 (38%), Positives = 68/121 (56%), Gaps = 13/121 (10%)
Frame = -3
Query: 576 GKLLENGLGWTAWLLHQAVV--NKEMSYEWINSRLQSLSLPRNTSVTKPGFIVQTGSPRF 403
G+LL+N LGW AW LH+AVV N + +++ L+S ++ + + P ++ SPRF
Sbjct: 327 GELLQNDLGWAAWKLHEAVVNHNDKSIRGLLDAWLRSPTVYQIGKLFDPYSVMMGSSPRF 386
Query: 402 DLHAIDFGLGKPVATRTGNA-----------GXXXXXXXDILICLPPHAMTSLESDKEFM 256
D + +FG+G+ +A R+G A G D+ CLPP M +LESDKEFM
Sbjct: 387 DKYGNEFGMGRALALRSGYAHKFDGKVSCYPGHEGGGSIDLETCLPPETMRALESDKEFM 446
Query: 255 E 253
E
Sbjct: 447 E 447