BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Forsythia22_contig00062928
         (346 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_009781463.1| PREDICTED: uncharacterized protein LOC104230...   111   2e-22
ref|XP_009781462.1| PREDICTED: uncharacterized protein LOC104230...   111   2e-22
ref|XP_009781379.1| PREDICTED: uncharacterized protein LOC104230...   109   6e-22
ref|XP_009795116.1| PREDICTED: uncharacterized protein LOC104241...   108   1e-21
ref|XP_006847242.1| PREDICTED: protein FAR1-RELATED SEQUENCE 4, ...   108   1e-21
ref|XP_008354295.1| PREDICTED: uncharacterized protein LOC103417...   108   1e-21
ref|XP_009772276.1| PREDICTED: uncharacterized protein LOC104222...   108   2e-21
ref|XP_008343020.1| PREDICTED: uncharacterized protein LOC103405...   107   2e-21
ref|XP_009777110.1| PREDICTED: protein FAR1-RELATED SEQUENCE 3-l...   107   3e-21
ref|XP_009785961.1| PREDICTED: protein FAR1-RELATED SEQUENCE 4-l...   107   3e-21
ref|XP_009801497.1| PREDICTED: uncharacterized protein LOC104247...   107   4e-21
ref|XP_009789484.1| PREDICTED: uncharacterized protein LOC104237...   107   4e-21
ref|XP_009792872.1| PREDICTED: protein FAR1-RELATED SEQUENCE 5-l...   107   4e-21
ref|XP_008384432.1| PREDICTED: uncharacterized protein LOC103447...   107   4e-21
ref|XP_008362838.1| PREDICTED: uncharacterized protein LOC103426...   106   5e-21
ref|XP_008338733.1| PREDICTED: uncharacterized protein LOC103401...   105   9e-21
ref|XP_010660160.1| PREDICTED: uncharacterized protein LOC100241...   105   1e-20
ref|XP_009785225.1| PREDICTED: uncharacterized protein LOC104233...   105   2e-20
ref|XP_010651472.1| PREDICTED: uncharacterized protein LOC100265...   104   2e-20
ref|XP_009779203.1| PREDICTED: uncharacterized protein LOC104228...   104   2e-20

>ref|XP_009781463.1| PREDICTED: uncharacterized protein LOC104230373 isoform X2
           [Nicotiana sylvestris]
          Length = 769

 Score =  111 bits (278), Expect = 2e-22
 Identities = 48/99 (48%), Positives = 72/99 (72%)
 Frame = -1

Query: 334 YILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKNGGTI 155
           Y   DI+ D+ + YG+ L+Y KAWRA++ ALE +  NP E YGK+P YL+ML   N G++
Sbjct: 450 YTPADIMRDVKKDYGVDLTYMKAWRAKQKALEILRGNPSESYGKLPSYLYMLMHTNPGSV 509

Query: 154 TRIKTNAENQFVYMFMALGASIQGWEHCKPIIVIDGTFL 38
            ++  +    F+Y+F++L ASI+GWE+CKPI+VI+G+FL
Sbjct: 510 VKLHKSEGGSFLYVFVSLDASIKGWEYCKPIVVINGSFL 548


>ref|XP_009781462.1| PREDICTED: uncharacterized protein LOC104230373 isoform X1
           [Nicotiana sylvestris]
          Length = 914

 Score =  111 bits (278), Expect = 2e-22
 Identities = 48/99 (48%), Positives = 72/99 (72%)
 Frame = -1

Query: 334 YILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKNGGTI 155
           Y   DI+ D+ + YG+ L+Y KAWRA++ ALE +  NP E YGK+P YL+ML   N G++
Sbjct: 450 YTPADIMRDVKKDYGVDLTYMKAWRAKQKALEILRGNPSESYGKLPSYLYMLMHTNPGSV 509

Query: 154 TRIKTNAENQFVYMFMALGASIQGWEHCKPIIVIDGTFL 38
            ++  +    F+Y+F++L ASI+GWE+CKPI+VI+G+FL
Sbjct: 510 VKLHKSEGGSFLYVFVSLDASIKGWEYCKPIVVINGSFL 548


>ref|XP_009781379.1| PREDICTED: uncharacterized protein LOC104230313 [Nicotiana
           sylvestris]
          Length = 472

 Score =  109 bits (273), Expect = 6e-22
 Identities = 48/109 (44%), Positives = 73/109 (66%)
 Frame = -1

Query: 343 KIIYILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKNG 164
           K +Y   DI  DM   +GL LSY +AWRA+E AL+ +  NP + Y K+P Y ++L+E   
Sbjct: 140 KTVYTPKDIQTDMLSEHGLNLSYMQAWRAKEKALQFLRGNPSDSYNKLPKYFYILEETYP 199

Query: 163 GTITRIKTNAENQFVYMFMALGASIQGWEHCKPIIVIDGTFLLDVMVGL 17
           G++ ++K  A++ F+Y F+AL  SI GW+HC+P++V+DGTFL     G+
Sbjct: 200 GSVVKLKKAADDCFLYAFVALCTSISGWQHCRPVVVVDGTFLKSAYRGI 248


>ref|XP_009795116.1| PREDICTED: uncharacterized protein LOC104241854 [Nicotiana
           sylvestris]
          Length = 559

 Score =  108 bits (271), Expect = 1e-21
 Identities = 46/102 (45%), Positives = 74/102 (72%)
 Frame = -1

Query: 343 KIIYILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKNG 164
           K IY   DII D+ + +G+ +SY KAWRA+E A+  I  +P + Y ++P Y +ML++ N 
Sbjct: 234 KKIYTANDIIEDIQKQHGIEVSYMKAWRAKEIAMTMIRGSPSDSYKELPKYFYMLEQTNS 293

Query: 163 GTITRIKTNAENQFVYMFMALGASIQGWEHCKPIIVIDGTFL 38
           GT+T++  + +  F+Y +++L ASI+GWEHC+PI+V+DG+FL
Sbjct: 294 GTVTKLHKSEDGCFLYTYVSLYASIKGWEHCRPIMVVDGSFL 335


>ref|XP_006847242.1| PREDICTED: protein FAR1-RELATED SEQUENCE 4, partial [Amborella
           trichopoda] gi|548850309|gb|ERN08823.1| hypothetical
           protein AMTR_s04652p00001680, partial [Amborella
           trichopoda]
          Length = 607

 Score =  108 bits (270), Expect(2) = 1e-21
 Identities = 49/95 (51%), Positives = 67/95 (70%)
 Frame = -1

Query: 322 DIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKNGGTITRIK 143
           DI  +M   YG+ ++Y KAWR++EHA E +     E Y  +P +LHMLQ+ N GTI  ++
Sbjct: 216 DIRGEMKHKYGVRMNYMKAWRSKEHAQEELRGKANESYRLLPGFLHMLQKTNPGTIVHME 275

Query: 142 TNAENQFVYMFMALGASIQGWEHCKPIIVIDGTFL 38
           T  +N F Y+F+AL ASI+GW+ CKPIIV+DGTFL
Sbjct: 276 TEDDNSFKYLFVALDASIKGWKKCKPIIVVDGTFL 310



 Score = 20.8 bits (42), Expect(2) = 1e-21
 Identities = 7/9 (77%), Positives = 8/9 (88%)
 Frame = -3

Query: 29  YGGTLFTAC 3
           YGGTL +AC
Sbjct: 314 YGGTLLSAC 322


>ref|XP_008354295.1| PREDICTED: uncharacterized protein LOC103417917 [Malus domestica]
          Length = 561

 Score =  108 bits (270), Expect = 1e-21
 Identities = 53/110 (48%), Positives = 72/110 (65%), Gaps = 1/110 (0%)
 Frame = -1

Query: 346 AKIIYILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKN 167
           A  +Y   DII DM    G+ +SY KAWRAREHA + I  +PEE +  +P Y  ML+ KN
Sbjct: 234 ASXVYRPKDIIEDMRXQVGVNMSYEKAWRAREHAFDMIRGSPEESFAXLPXYCAMLESKN 293

Query: 166 GGTITRIKTNAENQFVYMFMALGASIQGWE-HCKPIIVIDGTFLLDVMVG 20
            GTIT I+T+  N F+Y FMA+GASI+G+    +P++ +DGTFL    +G
Sbjct: 294 PGTITHIETDDNNHFLYFFMAMGASIRGFRGXXRPVVAVDGTFLKGKYLG 343


>ref|XP_009772276.1| PREDICTED: uncharacterized protein LOC104222701, partial [Nicotiana
           sylvestris]
          Length = 672

 Score =  108 bits (269), Expect = 2e-21
 Identities = 46/102 (45%), Positives = 72/102 (70%)
 Frame = -1

Query: 343 KIIYILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKNG 164
           K IY   DI  D+ + YG+ LSY +AWR++E A++ +  +P E Y K+P YL+ML+  N 
Sbjct: 257 KTIYTPTDIQRDIQKAYGMDLSYMQAWRSKEKAMQLLRRSPSESYKKMPTYLYMLEYANP 316

Query: 163 GTITRIKTNAENQFVYMFMALGASIQGWEHCKPIIVIDGTFL 38
           G++TR+ T  +  F+Y F+A+ ASI+GW +C+P +V+DG+FL
Sbjct: 317 GSVTRLHTEGDGSFLYAFIAIYASIRGWVYCRPTVVVDGSFL 358


>ref|XP_008343020.1| PREDICTED: uncharacterized protein LOC103405783 [Malus domestica]
          Length = 529

 Score =  107 bits (268), Expect = 2e-21
 Identities = 53/110 (48%), Positives = 72/110 (65%), Gaps = 1/110 (0%)
 Frame = -1

Query: 346 AKIIYILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKN 167
           A  +Y   DII DM    G+ +SY KAWRAREHA + I  +PEE +  +P Y  ML+ KN
Sbjct: 202 ASRVYRPKDIIEDMRAQVGVNMSYEKAWRAREHAFDMIRGSPEESFAALPAYCXMLEXKN 261

Query: 166 GGTITRIKTNAENQFVYMFMALGASIQGWE-HCKPIIVIDGTFLLDVMVG 20
            GTIT I+T+  N F+Y FMA+GASI+G+    +P++ +DGTFL    +G
Sbjct: 262 PGTITHIETDDNNHFLYFFMAMGASIRGFRGSMRPVVAVDGTFLKGKYLG 311


>ref|XP_009777110.1| PREDICTED: protein FAR1-RELATED SEQUENCE 3-like [Nicotiana
           sylvestris]
          Length = 367

 Score =  107 bits (267), Expect = 3e-21
 Identities = 46/109 (42%), Positives = 75/109 (68%)
 Frame = -1

Query: 343 KIIYILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKNG 164
           KIIY   DI  DM   +G+ L+Y +AWRA+E ALE +  +P + Y K+P YL++L++   
Sbjct: 15  KIIYTPKDIQADMLSEHGMNLTYMQAWRAKEKALEFLRGHPADSYNKLPSYLYILEKTYS 74

Query: 163 GTITRIKTNAENQFVYMFMALGASIQGWEHCKPIIVIDGTFLLDVMVGL 17
           G++ ++K   ++ F+Y+F+A+  SI GWE+C+P++V+DGTFL     G+
Sbjct: 75  GSVVKLKKTDDDCFMYVFVAICTSISGWEYCRPVVVVDGTFLKSAYTGI 123


>ref|XP_009785961.1| PREDICTED: protein FAR1-RELATED SEQUENCE 4-like [Nicotiana
           sylvestris]
          Length = 696

 Score =  107 bits (267), Expect = 3e-21
 Identities = 49/102 (48%), Positives = 71/102 (69%)
 Frame = -1

Query: 343 KIIYILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKNG 164
           K IY   DI+ DM + YG+ LSY KA R+++ AL+ +  +P E Y K+P YL+ML   N 
Sbjct: 228 KTIYTAKDIMKDMKKHYGIDLSYWKANRSKQKALQFLRGDPSESYAKLPSYLYMLMHSNP 287

Query: 163 GTITRIKTNAENQFVYMFMALGASIQGWEHCKPIIVIDGTFL 38
           G++  ++ + E  F+Y F+AL ASI+GWE CKPI+V+DG+FL
Sbjct: 288 GSVVCLRKSDEEHFMYAFVALYASIKGWEFCKPIVVVDGSFL 329


>ref|XP_009801497.1| PREDICTED: uncharacterized protein LOC104247235 [Nicotiana
           sylvestris]
          Length = 247

 Score =  107 bits (266), Expect = 4e-21
 Identities = 46/102 (45%), Positives = 72/102 (70%)
 Frame = -1

Query: 343 KIIYILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKNG 164
           K +Y   DII D+ + +G+ +SY KAWRA+E A   I  NP + Y ++P Y +ML+  N 
Sbjct: 5   KKVYTANDIIEDIQKQHGIEVSYIKAWRAKEIAKAMIRGNPNDSYKELPKYFYMLEHTNP 64

Query: 163 GTITRIKTNAENQFVYMFMALGASIQGWEHCKPIIVIDGTFL 38
           GT+T++  + +  F+Y +++L ASI+GWEHC+PI+V+DG+FL
Sbjct: 65  GTVTKLHKSEDGCFLYAYVSLYASIKGWEHCRPIMVVDGSFL 106


>ref|XP_009789484.1| PREDICTED: uncharacterized protein LOC104237100 [Nicotiana
           sylvestris]
          Length = 718

 Score =  107 bits (266), Expect = 4e-21
 Identities = 47/109 (43%), Positives = 73/109 (66%)
 Frame = -1

Query: 343 KIIYILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKNG 164
           K +Y   DI  DM   +GL LSY +AWRA+E AL+ +  NP + Y K+P Y ++L++   
Sbjct: 249 KTVYTPKDIQTDMLSEHGLNLSYMQAWRAKEKALQFLRGNPCDSYNKLPKYFYILEKNYP 308

Query: 163 GTITRIKTNAENQFVYMFMALGASIQGWEHCKPIIVIDGTFLLDVMVGL 17
           G++ ++K  A++ F+Y F+AL  SI GW+HC+P++V+DGTFL     G+
Sbjct: 309 GSVVKLKKAADDCFLYAFVALCTSISGWQHCRPVVVVDGTFLKSAYRGI 357


>ref|XP_009792872.1| PREDICTED: protein FAR1-RELATED SEQUENCE 5-like [Nicotiana
           sylvestris] gi|698456296|ref|XP_009780587.1| PREDICTED:
           protein FAR1-RELATED SEQUENCE 5-like [Nicotiana
           sylvestris]
          Length = 511

 Score =  107 bits (266), Expect = 4e-21
 Identities = 47/109 (43%), Positives = 73/109 (66%)
 Frame = -1

Query: 343 KIIYILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKNG 164
           K +Y   DI  DM   +GL LSY +AWRA+E AL+ +  NP + Y K+P Y ++L++   
Sbjct: 42  KTVYTPKDIQTDMLSEHGLNLSYMQAWRAKEKALQFLRGNPCDSYNKLPKYFYILEKNYP 101

Query: 163 GTITRIKTNAENQFVYMFMALGASIQGWEHCKPIIVIDGTFLLDVMVGL 17
           G++ ++K  A++ F+Y F+AL  SI GW+HC+P++V+DGTFL     G+
Sbjct: 102 GSVVKLKKAADDCFLYAFVALCTSINGWQHCRPVVVVDGTFLKSAYRGI 150


>ref|XP_008384432.1| PREDICTED: uncharacterized protein LOC103447035 [Malus domestica]
          Length = 487

 Score =  107 bits (266), Expect = 4e-21
 Identities = 52/110 (47%), Positives = 71/110 (64%), Gaps = 1/110 (0%)
 Frame = -1

Query: 346 AKIIYILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKN 167
           A  +Y   D I DM    G+ +SY KAWRAREHA + I  +PEE +  +P Y  ML+ KN
Sbjct: 234 ASXVYRPKDXIEDMRXQVGVNMSYXKAWRAREHAFDMIXGSPEESFAXLPXYCXMLEXKN 293

Query: 166 GGTITRIKTNAENQFVYMFMALGASIQGWE-HCKPIIVIDGTFLLDVMVG 20
            GTIT I+T+  N F+Y FMA+GASI+G+    +P++ +DGTFL    +G
Sbjct: 294 PGTITHIETDDNNHFLYFFMAMGASIRGFRGSXRPVVAVDGTFLKGKYLG 343


>ref|XP_008362838.1| PREDICTED: uncharacterized protein LOC103426531 [Malus domestica]
          Length = 486

 Score =  106 bits (265), Expect = 5e-21
 Identities = 52/110 (47%), Positives = 71/110 (64%), Gaps = 1/110 (0%)
 Frame = -1

Query: 346 AKIIYILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKN 167
           A  +Y   D I DM    G+ +SY KAWRAREHA + I  +PEE +  +P Y  ML+ KN
Sbjct: 234 ASXVYRPKDXIEDMRAQVGVNMSYEKAWRAREHAFDMIRGSPEESFAALPXYCAMLESKN 293

Query: 166 GGTITRIKTNAENQFVYMFMALGASIQGWE-HCKPIIVIDGTFLLDVMVG 20
            GTIT I+T+  N F+Y FMA+GASI+G+    +P++ +DGTFL    +G
Sbjct: 294 PGTITHIETDDNNHFLYFFMAMGASIRGFRGSXRPVVAVDGTFLKGKYLG 343


>ref|XP_008338733.1| PREDICTED: uncharacterized protein LOC103401792 [Malus domestica]
          Length = 486

 Score =  105 bits (263), Expect = 9e-21
 Identities = 52/110 (47%), Positives = 71/110 (64%), Gaps = 1/110 (0%)
 Frame = -1

Query: 346 AKIIYILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKN 167
           A  +Y   D I DM    G+ +SY KAWRAREHA + I  +PEE +  +P Y  ML+ KN
Sbjct: 234 ASRVYRPKDXIEDMRAQVGVNMSYEKAWRAREHAFDMIRGSPEESFAALPAYCXMLEXKN 293

Query: 166 GGTITRIKTNAENQFVYMFMALGASIQGWE-HCKPIIVIDGTFLLDVMVG 20
            GTIT I+T+  N F+Y FMA+GASI+G+    +P++ +DGTFL    +G
Sbjct: 294 PGTITHIETDDNNHFLYFFMAMGASIRGFRGSMRPVVAVDGTFLKGKYLG 343


>ref|XP_010660160.1| PREDICTED: uncharacterized protein LOC100241533 [Vitis vinifera]
          Length = 487

 Score =  105 bits (262), Expect = 1e-20
 Identities = 49/98 (50%), Positives = 71/98 (72%), Gaps = 1/98 (1%)
 Frame = -1

Query: 328 LGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKNGGTITR 149
           L DI+ D+ + YG+ +SY KAWRA+E AL SI  +PEE Y  +P Y ++L++KN GTIT 
Sbjct: 30  LKDIVADIRKQYGIQISYDKAWRAKELALGSIRGSPEESYNTLPSYCYVLEQKNPGTITD 89

Query: 148 IKTNAENQFVYMFMALGASIQGWE-HCKPIIVIDGTFL 38
           I T+ +NQF Y FM++GAS+ G+    +P++ +DGTFL
Sbjct: 90  IVTDCDNQFKYFFMSIGASLAGFHTSIRPVVAVDGTFL 127


>ref|XP_009785225.1| PREDICTED: uncharacterized protein LOC104233522, partial [Nicotiana
           sylvestris]
          Length = 364

 Score =  105 bits (261), Expect = 2e-20
 Identities = 46/109 (42%), Positives = 72/109 (66%)
 Frame = -1

Query: 343 KIIYILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKNG 164
           K +Y   DI  DM   +GL LSY +AWRA+E AL+ +  NP + Y K+P Y ++L++   
Sbjct: 140 KTVYTPKDIQTDMLSEHGLNLSYMQAWRAKEKALQFLRGNPCDSYNKLPKYFYILEKNYP 199

Query: 163 GTITRIKTNAENQFVYMFMALGASIQGWEHCKPIIVIDGTFLLDVMVGL 17
           G++ ++K   ++ F+Y F+AL  SI GW+HC+P++V+DGTFL     G+
Sbjct: 200 GSVVKLKKAEDDCFLYAFVALCTSINGWQHCRPVVVVDGTFLKSAYRGI 248


>ref|XP_010651472.1| PREDICTED: uncharacterized protein LOC100265612 [Vitis vinifera]
          Length = 494

 Score =  104 bits (260), Expect = 2e-20
 Identities = 48/96 (50%), Positives = 70/96 (72%), Gaps = 1/96 (1%)
 Frame = -1

Query: 322 DIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKNGGTITRIK 143
           DI+ D+ + YG+ +SY KAWRA+E AL SI  +PEE Y  +P Y ++L++KN GTIT I 
Sbjct: 243 DIVADIRKQYGIQISYDKAWRAKELALGSIRGSPEESYNTLPSYCYVLEQKNPGTITDIV 302

Query: 142 TNAENQFVYMFMALGASIQGWE-HCKPIIVIDGTFL 38
           T+ +NQF Y FM++GAS+ G+    +P++ +DGTFL
Sbjct: 303 TDCDNQFKYFFMSIGASLAGFHTSIRPVVAVDGTFL 338


>ref|XP_009779203.1| PREDICTED: uncharacterized protein LOC104228439 isoform X1
           [Nicotiana sylvestris] gi|698587500|ref|XP_009779204.1|
           PREDICTED: uncharacterized protein LOC104228439 isoform
           X1 [Nicotiana sylvestris]
          Length = 547

 Score =  104 bits (260), Expect = 2e-20
 Identities = 44/102 (43%), Positives = 71/102 (69%)
 Frame = -1

Query: 343 KIIYILGDIINDMARTYGLTLSYGKAWRAREHALESIMENPEELYGKVPMYLHMLQEKNG 164
           K +Y   DI  D+ + +G  ++Y KAWR++E ALE +  NP + Y ++P YL+ML   N 
Sbjct: 180 KTVYTPADIRRDIKKIHGFDVNYMKAWRSKEKALEMVRGNPSKSYTELPNYLYMLMHTNP 239

Query: 163 GTITRIKTNAENQFVYMFMALGASIQGWEHCKPIIVIDGTFL 38
           G++ R++   +  F+Y+F+A+ A I+GWE+CKPI+V+DG+FL
Sbjct: 240 GSVYRLEKTDDGSFLYLFVAIYALIKGWEYCKPIVVVDGSFL 281