BLASTX nr result
ID: Forsythia22_contig00058550
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Forsythia22_contig00058550
(356 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_011079437.1| PREDICTED: probable receptor-like serine/thr... 89 1e-15
emb|CDP02933.1| unnamed protein product [Coffea canephora] 86 7e-15
ref|XP_012065277.1| PREDICTED: probable receptor-like serine/thr... 82 1e-13
gb|KHG15349.1| hypothetical protein F383_17411 [Gossypium arboreum] 76 8e-12
ref|XP_007014101.1| LRR receptor-like serine/threonine-protein k... 75 1e-11
gb|KJB76587.1| hypothetical protein B456_012G095500 [Gossypium r... 75 2e-11
ref|XP_012460813.1| PREDICTED: probable receptor-like serine/thr... 75 2e-11
ref|XP_006381845.1| hypothetical protein POPTR_0006s18770g [Popu... 75 2e-11
ref|XP_011018161.1| PREDICTED: probable receptor-like serine/thr... 74 4e-11
ref|XP_011012684.1| PREDICTED: probable receptor-like serine/thr... 72 1e-10
ref|XP_011012683.1| PREDICTED: probable receptor-like serine/thr... 72 1e-10
ref|XP_002325078.2| hypothetical protein POPTR_0018s10520g [Popu... 72 1e-10
ref|XP_002534342.1| conserved hypothetical protein [Ricinus comm... 68 3e-09
gb|KHN17563.1| Putative receptor-like serine/threonine-protein k... 65 2e-08
ref|XP_006600504.1| PREDICTED: probable receptor-like serine/thr... 65 2e-08
ref|XP_003550670.1| PREDICTED: probable receptor-like serine/thr... 65 2e-08
ref|XP_006593554.1| PREDICTED: probable receptor-like serine/thr... 64 4e-08
ref|XP_003543756.1| PREDICTED: probable receptor-like serine/thr... 64 4e-08
ref|XP_010656799.1| PREDICTED: probable receptor-like serine/thr... 63 7e-08
ref|XP_009608072.1| PREDICTED: probable receptor-like serine/thr... 63 7e-08
>ref|XP_011079437.1| PREDICTED: probable receptor-like serine/threonine-protein kinase
At5g57670 [Sesamum indicum]
Length = 603
Score = 89.0 bits (219), Expect = 1e-15
Identities = 56/120 (46%), Positives = 73/120 (60%), Gaps = 3/120 (2%)
Frame = +1
Query: 4 SFSSEENEIYSKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKR 183
SFSS+ENEI S + + PSP TVL C EDSSS E+ + + +S + ELK
Sbjct: 181 SFSSDENEISSNMKSKKPSPRTVLDVC--------EDSSSSGETESCSTVTSLMATELKG 232
Query: 184 KPQIRSPKWILKQISYLFR-SSVDWSGGNRN--VTSLKEKQQSLLRCFTYQEIANATNNF 354
K + R P K IS+LFR S DWS +N + + +++Q+ LLRCFTY EIA ATNNF
Sbjct: 233 KQESRKPMSPFKLISFLFRPPSFDWSTRKQNDALFNKQKQQRPLLRCFTYHEIARATNNF 292
>emb|CDP02933.1| unnamed protein product [Coffea canephora]
Length = 900
Score = 86.3 bits (212), Expect = 7e-15
Identities = 57/118 (48%), Positives = 66/118 (55%), Gaps = 1/118 (0%)
Frame = +1
Query: 4 SFSSEENEIYSKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKR 183
S SS+E SK + E SP TVL AC ESQ T ED SS+ ES S + E KR
Sbjct: 477 SVSSDEERAGSKAKTEKRSPRTVLDACERESQGTMEDGSSIDESSIRK--SPYVASESKR 534
Query: 184 KPQIRSPKWILKQISYLFRSSVDWSGGNRNVTSL-KEKQQSLLRCFTYQEIANATNNF 354
+ + + P LK IS FRS D S RN T K KQQ +CF Y+EIANATNNF
Sbjct: 535 QSETKRPMSPLKIISSFFRSPFDSSARKRNDTFFNKNKQQPTFKCFPYEEIANATNNF 592
>ref|XP_012065277.1| PREDICTED: probable receptor-like serine/threonine-protein kinase
At5g57670 [Jatropha curcas] gi|643737760|gb|KDP43801.1|
hypothetical protein JCGZ_23009 [Jatropha curcas]
Length = 590
Score = 82.0 bits (201), Expect = 1e-13
Identities = 55/117 (47%), Positives = 71/117 (60%), Gaps = 2/117 (1%)
Frame = +1
Query: 10 SSEENEIYSKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKRKP 189
S+ E + SK + +NPSP TVL GES ST++D+ S S +T SS S+ K KP
Sbjct: 169 SNGEKDTESKIKIQNPSPRTVLDELDGESHSTEDDTISFEGSSSTE--SSSPSLPSKLKP 226
Query: 190 QIRSPKWI--LKQISYLFRSSVDWSGGNRNVTSLKEKQQSLLRCFTYQEIANATNNF 354
+ +S K + K IS F SS+ + S KEKQQ LLRCF+Y+EIANATNNF
Sbjct: 227 ESKSRKQMYGCKIISSFFTSSLRKRTAS---FSKKEKQQPLLRCFSYEEIANATNNF 280
>gb|KHG15349.1| hypothetical protein F383_17411 [Gossypium arboreum]
Length = 605
Score = 76.3 bits (186), Expect = 8e-12
Identities = 48/120 (40%), Positives = 71/120 (59%), Gaps = 3/120 (2%)
Frame = +1
Query: 4 SFSSEENEIYSKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKR 183
S S +N I SK + + PSP TVL A +GES ST++D+SS +S + + P
Sbjct: 183 SISPVQNSIVSKRKLQKPSPRTVLDALLGESNSTEDDNSSFWDSTMSGSPPHP------- 235
Query: 184 KPQIRSPKWILKQIS-YLFRSSVDWSGGNRNVTSL--KEKQQSLLRCFTYQEIANATNNF 354
P+ + +I KQ+S Y F S V S + T+L K+K Q L++CF+++EI+ ATNNF
Sbjct: 236 -PKFKGQSFIKKQMSPYKFVSMVFRSPLRKRNTNLSNKQKAQPLMKCFSFEEISTATNNF 294
>ref|XP_007014101.1| LRR receptor-like serine/threonine-protein kinase [Theobroma cacao]
gi|508784464|gb|EOY31720.1| LRR receptor-like
serine/threonine-protein kinase [Theobroma cacao]
Length = 623
Score = 75.5 bits (184), Expect = 1e-11
Identities = 50/115 (43%), Positives = 71/115 (61%), Gaps = 3/115 (2%)
Frame = +1
Query: 19 ENEIYSKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKRKPQIR 198
+N I SK + + PSP TVL A GES ST++++SS +S T + S P R P+ +
Sbjct: 207 QNSIVSKRKLQKPSPRTVLKALEGESNSTEDETSSFGDS--TISGSPP------RAPKFK 258
Query: 199 SPKWILKQIS-YLFRSSVDWSGGNRNVTSL--KEKQQSLLRCFTYQEIANATNNF 354
I+KQ+S Y SSV S + SL KEK++ L++CF+Y+EI+NATNNF
Sbjct: 259 RQFCIMKQMSPYKLISSVFSSPLRKRKASLSNKEKEEPLIKCFSYEEISNATNNF 313
>gb|KJB76587.1| hypothetical protein B456_012G095500 [Gossypium raimondii]
Length = 611
Score = 75.1 bits (183), Expect = 2e-11
Identities = 48/117 (41%), Positives = 68/117 (58%)
Frame = +1
Query: 4 SFSSEENEIYSKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKR 183
S S +N I SK + + PSP TVL A GES ST++DSSS +S T + S P + K
Sbjct: 186 SISPVQNSIVSKQKLQKPSPRTVLDALQGESNSTEDDSSSFWDS--TMSGSPPQPPKFKG 243
Query: 184 KPQIRSPKWILKQISYLFRSSVDWSGGNRNVTSLKEKQQSLLRCFTYQEIANATNNF 354
+ I+ K +S +FRS + N S K+K Q L++CF+++EI+ ATNNF
Sbjct: 244 QSCIKKQMSPYKFVSMVFRSPLRKRNAN---FSNKQKGQPLMKCFSFEEISTATNNF 297
>ref|XP_012460813.1| PREDICTED: probable receptor-like serine/threonine-protein kinase
At5g57670 [Gossypium raimondii]
gi|763809684|gb|KJB76586.1| hypothetical protein
B456_012G095500 [Gossypium raimondii]
Length = 608
Score = 75.1 bits (183), Expect = 2e-11
Identities = 48/117 (41%), Positives = 68/117 (58%)
Frame = +1
Query: 4 SFSSEENEIYSKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKR 183
S S +N I SK + + PSP TVL A GES ST++DSSS +S T + S P + K
Sbjct: 186 SISPVQNSIVSKQKLQKPSPRTVLDALQGESNSTEDDSSSFWDS--TMSGSPPQPPKFKG 243
Query: 184 KPQIRSPKWILKQISYLFRSSVDWSGGNRNVTSLKEKQQSLLRCFTYQEIANATNNF 354
+ I+ K +S +FRS + N S K+K Q L++CF+++EI+ ATNNF
Sbjct: 244 QSCIKKQMSPYKFVSMVFRSPLRKRNAN---FSNKQKGQPLMKCFSFEEISTATNNF 297
>ref|XP_006381845.1| hypothetical protein POPTR_0006s18770g [Populus trichocarpa]
gi|550336603|gb|ERP59642.1| hypothetical protein
POPTR_0006s18770g [Populus trichocarpa]
Length = 613
Score = 74.7 bits (182), Expect = 2e-11
Identities = 50/116 (43%), Positives = 67/116 (57%), Gaps = 1/116 (0%)
Frame = +1
Query: 10 SSEENEIYSKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKRKP 189
S+ EN I S+ E +N SP TVL ES ST++D+ S +S TT S P + + +
Sbjct: 194 STIENHINSETEKQNSSPRTVLHQLEAESHSTEDDTLSFGDSSTTE--SPPLATNINGRS 251
Query: 190 QIRSPKWILKQISYLFRSSVDWSGGNRNVTSL-KEKQQSLLRCFTYQEIANATNNF 354
+ + K IS +F S + RN L KEKQQ LL+CFT++EIANATNNF
Sbjct: 252 KTKKQISTCKLISSIFASPMR----KRNRIFLNKEKQQPLLKCFTFEEIANATNNF 303
>ref|XP_011018161.1| PREDICTED: probable receptor-like serine/threonine-protein kinase
At5g57670 [Populus euphratica]
Length = 597
Score = 73.9 bits (180), Expect = 4e-11
Identities = 52/117 (44%), Positives = 72/117 (61%), Gaps = 2/117 (1%)
Frame = +1
Query: 10 SSEENEIYSKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNN--FSSPGSIELKR 183
SS E I S+ + N SP TVL ES ST++D+ S+ S TT + ++ +++ K
Sbjct: 178 SSVEEHINSETKRLNSSPRTVLYEIETESHSTEDDTFSIGGSSTTESPPLATNFNVQSKT 237
Query: 184 KPQIRSPKWILKQISYLFRSSVDWSGGNRNVTSLKEKQQSLLRCFTYQEIANATNNF 354
K Q + K IS +F S V NR+V++ K+KQQSLL+CFTY+EIANATNNF
Sbjct: 238 KKQTST----CKLISSIFASPVRKM--NRSVSN-KQKQQSLLKCFTYEEIANATNNF 287
>ref|XP_011012684.1| PREDICTED: probable receptor-like serine/threonine-protein kinase
At5g57670 isoform X2 [Populus euphratica]
Length = 603
Score = 72.0 bits (175), Expect = 1e-10
Identities = 50/116 (43%), Positives = 67/116 (57%), Gaps = 1/116 (0%)
Frame = +1
Query: 10 SSEENEIYSKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKRKP 189
S+ EN I S+ + +N SP TVL ES ST++D+ S +S TT S P + + +
Sbjct: 184 STIENLINSETKRQNSSPRTVLHQIEAESHSTEDDTLSFGDSSTTE--SPPLATNINGRS 241
Query: 190 QIRSPKWILKQISYLFRSSVDWSGGNRNVTSL-KEKQQSLLRCFTYQEIANATNNF 354
+ + K IS LF S + RN L KEKQQ LL+CFT++EIANATNNF
Sbjct: 242 KTKKQISNCKLISSLFASPMR----KRNRIFLNKEKQQPLLKCFTFEEIANATNNF 293
>ref|XP_011012683.1| PREDICTED: probable receptor-like serine/threonine-protein kinase
At5g57670 isoform X1 [Populus euphratica]
Length = 604
Score = 72.0 bits (175), Expect = 1e-10
Identities = 50/116 (43%), Positives = 67/116 (57%), Gaps = 1/116 (0%)
Frame = +1
Query: 10 SSEENEIYSKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKRKP 189
S+ EN I S+ + +N SP TVL ES ST++D+ S +S TT S P + + +
Sbjct: 185 STIENLINSETKRQNSSPRTVLHQIEAESHSTEDDTLSFGDSSTTE--SPPLATNINGRS 242
Query: 190 QIRSPKWILKQISYLFRSSVDWSGGNRNVTSL-KEKQQSLLRCFTYQEIANATNNF 354
+ + K IS LF S + RN L KEKQQ LL+CFT++EIANATNNF
Sbjct: 243 KTKKQISNCKLISSLFASPMR----KRNRIFLNKEKQQPLLKCFTFEEIANATNNF 294
>ref|XP_002325078.2| hypothetical protein POPTR_0018s10520g [Populus trichocarpa]
gi|550318458|gb|EEF03643.2| hypothetical protein
POPTR_0018s10520g [Populus trichocarpa]
Length = 597
Score = 72.0 bits (175), Expect = 1e-10
Identities = 50/115 (43%), Positives = 67/115 (58%)
Frame = +1
Query: 10 SSEENEIYSKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKRKP 189
SS E I S+ + N SP TVL ES ST++D+ S S TT S P + +
Sbjct: 178 SSVEKHINSETKRLNSSPRTVLYEIETESHSTEDDTFSFGGSSTTE--SPPLATNFNGQS 235
Query: 190 QIRSPKWILKQISYLFRSSVDWSGGNRNVTSLKEKQQSLLRCFTYQEIANATNNF 354
+ + K IS +F S + NR+V++ K+KQQSLL+CFTY+EIANATNNF
Sbjct: 236 KTKKQTSTCKLISSIFASPMRKI--NRSVSN-KQKQQSLLKCFTYEEIANATNNF 287
>ref|XP_002534342.1| conserved hypothetical protein [Ricinus communis]
gi|223525458|gb|EEF28040.1| conserved hypothetical
protein [Ricinus communis]
Length = 563
Score = 67.8 bits (164), Expect = 3e-09
Identities = 47/118 (39%), Positives = 67/118 (56%)
Frame = +1
Query: 1 NSFSSEENEIYSKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELK 180
+S ++N +K + ++PSP TVL GES ST++DS S+ S ++ SP + +
Sbjct: 184 DSLVEKDNGSETKSQ-KSPSPRTVLAEVDGESHSTEDDSISIEGS---SSIESPLPPKFR 239
Query: 181 RKPQIRSPKWILKQISYLFRSSVDWSGGNRNVTSLKEKQQSLLRCFTYQEIANATNNF 354
+ R K IS +F S + G+ S KEKQQ LL CF+Y+EIANATNNF
Sbjct: 240 TGSKSRKQISACKIISSIFTSPLRKRNGS---FSNKEKQQPLLNCFSYEEIANATNNF 294
>gb|KHN17563.1| Putative receptor-like serine/threonine-protein kinase [Glycine
soja]
Length = 605
Score = 65.1 bits (157), Expect = 2e-08
Identities = 40/107 (37%), Positives = 64/107 (59%)
Frame = +1
Query: 34 SKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKRKPQIRSPKWI 213
S + +N SP TVL G+S ST++D+ S S TT +P S + KR+ +IR P++
Sbjct: 195 SGSKAQNRSPRTVLNGLEGQSNSTEDDTFSTRVSSTTY---TPSSSDSKRRSKIRKPQFS 251
Query: 214 LKQISYLFRSSVDWSGGNRNVTSLKEKQQSLLRCFTYQEIANATNNF 354
+ I+ S + N ++ S EK+Q LL+CF+Y++I+NAT +F
Sbjct: 252 FRFIASFLASP--FRSKNFSI-SKNEKRQPLLKCFSYEQISNATKDF 295
>ref|XP_006600504.1| PREDICTED: probable receptor-like serine/threonine-protein kinase
At5g57670-like isoform X2 [Glycine max]
Length = 606
Score = 65.1 bits (157), Expect = 2e-08
Identities = 40/107 (37%), Positives = 64/107 (59%)
Frame = +1
Query: 34 SKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKRKPQIRSPKWI 213
S + +N SP TVL G+S ST++D+ S S TT +P S + KR+ +IR P++
Sbjct: 196 SGSKAQNRSPRTVLNGLEGQSNSTEDDTFSTRVSSTTY---TPSSSDSKRRSKIRKPQFS 252
Query: 214 LKQISYLFRSSVDWSGGNRNVTSLKEKQQSLLRCFTYQEIANATNNF 354
+ I+ S + N ++ S EK+Q LL+CF+Y++I+NAT +F
Sbjct: 253 FRFIASFLASP--FRSKNFSI-SKNEKRQPLLKCFSYEQISNATKDF 296
>ref|XP_003550670.1| PREDICTED: probable receptor-like serine/threonine-protein kinase
At5g57670-like isoform X1 [Glycine max]
Length = 605
Score = 65.1 bits (157), Expect = 2e-08
Identities = 40/107 (37%), Positives = 64/107 (59%)
Frame = +1
Query: 34 SKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKRKPQIRSPKWI 213
S + +N SP TVL G+S ST++D+ S S TT +P S + KR+ +IR P++
Sbjct: 195 SGSKAQNRSPRTVLNGLEGQSNSTEDDTFSTRVSSTTY---TPSSSDSKRRSKIRKPQFS 251
Query: 214 LKQISYLFRSSVDWSGGNRNVTSLKEKQQSLLRCFTYQEIANATNNF 354
+ I+ S + N ++ S EK+Q LL+CF+Y++I+NAT +F
Sbjct: 252 FRFIASFLASP--FRSKNFSI-SKNEKRQPLLKCFSYEQISNATKDF 295
>ref|XP_006593554.1| PREDICTED: probable receptor-like serine/threonine-protein kinase
At5g57670-like isoform X2 [Glycine max]
Length = 605
Score = 63.9 bits (154), Expect = 4e-08
Identities = 43/111 (38%), Positives = 66/111 (59%), Gaps = 4/111 (3%)
Frame = +1
Query: 34 SKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKRKPQIRSP--- 204
S + +N SP TVL G+S ST++D+ S S TTN SS + KR+ +IR P
Sbjct: 196 SGTKAQNRSPRTVLDGLEGQSNSTEDDTFSTRVSSTTNTPSS----DSKRRSKIRKPQFP 251
Query: 205 -KWILKQISYLFRSSVDWSGGNRNVTSLKEKQQSLLRCFTYQEIANATNNF 354
++I+ ++ FRS N ++ S EK+Q LL+CF+Y++I+NAT +F
Sbjct: 252 FRFIVSFLASPFRSK------NFSI-SKNEKRQPLLKCFSYEQISNATKDF 295
>ref|XP_003543756.1| PREDICTED: probable receptor-like serine/threonine-protein kinase
At5g57670-like isoform X1 [Glycine max]
gi|734408461|gb|KHN34789.1| Putative receptor-like
serine/threonine-protein kinase [Glycine soja]
Length = 604
Score = 63.9 bits (154), Expect = 4e-08
Identities = 43/111 (38%), Positives = 66/111 (59%), Gaps = 4/111 (3%)
Frame = +1
Query: 34 SKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKRKPQIRSP--- 204
S + +N SP TVL G+S ST++D+ S S TTN SS + KR+ +IR P
Sbjct: 195 SGTKAQNRSPRTVLDGLEGQSNSTEDDTFSTRVSSTTNTPSS----DSKRRSKIRKPQFP 250
Query: 205 -KWILKQISYLFRSSVDWSGGNRNVTSLKEKQQSLLRCFTYQEIANATNNF 354
++I+ ++ FRS N ++ S EK+Q LL+CF+Y++I+NAT +F
Sbjct: 251 FRFIVSFLASPFRSK------NFSI-SKNEKRQPLLKCFSYEQISNATKDF 294
>ref|XP_010656799.1| PREDICTED: probable receptor-like serine/threonine-protein kinase
At5g57670 [Vitis vinifera]
Length = 602
Score = 63.2 bits (152), Expect = 7e-08
Identities = 47/116 (40%), Positives = 62/116 (53%), Gaps = 1/116 (0%)
Frame = +1
Query: 10 SSEENEIYSKPECENPSPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKRKP 189
S+ + + SK + E + VL GES +T EDS S +S T S P + +LK +
Sbjct: 180 SAVQKLVTSKSKREKSTRKAVLDGSEGESHTT-EDSFSFGDSSITE--SPPLASKLKGQS 236
Query: 190 QIRSPKWILKQISYLFRSSVDWSGGNRNVT-SLKEKQQSLLRCFTYQEIANATNNF 354
R +K IS S + SG RN S KEK Q LLRCF+Y+EI+ ATNNF
Sbjct: 237 NFRKQLSPIKIISSFLLSPFNLSGRKRNERLSDKEKHQPLLRCFSYEEISKATNNF 292
>ref|XP_009608072.1| PREDICTED: probable receptor-like serine/threonine-protein kinase
At5g57670 isoform X2 [Nicotiana tomentosiformis]
Length = 564
Score = 63.2 bits (152), Expect = 7e-08
Identities = 42/100 (42%), Positives = 55/100 (55%), Gaps = 1/100 (1%)
Frame = +1
Query: 58 SPTTVLGACIGESQSTDEDSSSLAESCTTNNFSSPGSIELKRKPQIRSPKWILKQISYLF 237
SP TVL C GE EDSSS ES T + SS S ++ + P L++IS
Sbjct: 160 SPKTVLNGCEGE-----EDSSSFGESSITKSSSSVSSQFKVQQEYFKKPMSPLRRISSFL 214
Query: 238 RSSVDWSGGNRNVT-SLKEKQQSLLRCFTYQEIANATNNF 354
RS D S ++ + KEKQQ L+CF+Y+EIA +TN F
Sbjct: 215 RSPFDSSSRKKSASFQHKEKQQPPLKCFSYEEIACSTNYF 254