BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Forsythia22_contig00015973
         (1124 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011071002.1| PREDICTED: sphinganine C(4)-monooxygenase 1-...   462   e-127
ref|XP_012855270.1| PREDICTED: sphinganine C(4)-monooxygenase 1 ...   454   e-125
ref|XP_004238795.1| PREDICTED: sphinganine C(4)-monooxygenase 1-...   436   e-119
gb|ABO93014.1| putative sterol desaturase [Solanum tuberosum]         436   e-119
ref|XP_011075497.1| PREDICTED: sphinganine C(4)-monooxygenase 1-...   435   e-119
ref|XP_006363216.1| PREDICTED: sphinganine C(4)-monooxygenase 1-...   435   e-119
ref|XP_009793103.1| PREDICTED: sphinganine C(4)-monooxygenase 1-...   433   e-118
ref|XP_006366702.1| PREDICTED: sphinganine C(4)-monooxygenase 1-...   432   e-118
ref|XP_009758683.1| PREDICTED: sphinganine C(4)-monooxygenase 1-...   432   e-118
ref|XP_009626041.1| PREDICTED: sphinganine C(4)-monooxygenase 1-...   432   e-118
ref|XP_004228378.1| PREDICTED: sphinganine C(4)-monooxygenase 1-...   432   e-118
gb|ABO92998.1| putative sterol desaturase [Solanum tuberosum]         432   e-118
emb|CDP00325.1| unnamed protein product [Coffea canephora]            432   e-118
ref|XP_002275229.1| PREDICTED: sphinganine C(4)-monooxygenase 1 ...   432   e-118
ref|XP_012067630.1| PREDICTED: sphinganine C(4)-monooxygenase 1 ...   431   e-118
gb|KDP41189.1| hypothetical protein JCGZ_15596 [Jatropha curcas]      431   e-118
gb|EPS64083.1| hypothetical protein M569_10698 [Genlisea aurea]       431   e-118
ref|XP_009596162.1| PREDICTED: sphinganine C(4)-monooxygenase 1 ...   428   e-117
ref|XP_007046113.1| Sphingoid base hydroxylase 2 [Theobroma caca...   428   e-117
ref|XP_010089753.1| Sphingoid base hydroxylase 2 [Morus notabili...   427   e-117

>ref|XP_011071002.1| PREDICTED: sphinganine C(4)-monooxygenase 1-like [Sesamum indicum]
          Length = 263

 Score =  462 bits (1190), Expect = e-127
 Identities = 214/263 (81%), Positives = 239/263 (90%)
 Frame = -2

Query: 982 MQIIKGGLEISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKDVV 803
           M+++ GG ++SDE+LGTFAP+++YW YSGIY++LGSLE YRLHSKKDEDEKNLV KKDVV
Sbjct: 1   MEMMNGGFKVSDELLGTFAPIILYWAYSGIYVLLGSLEGYRLHSKKDEDEKNLVPKKDVV 60

Query: 802 KGXXXXXXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHRYM 623
           KG           ATILFAVTG+DSE+A AQ +SLIVL R+ +VAM++LDTWQYFMHRYM
Sbjct: 61  KGVLLQQAVQAVVATILFAVTGSDSESAQAQQSSLIVLARRFLVAMMVLDTWQYFMHRYM 120

Query: 622 HVNKFLYRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFFFS 443
           H NKFLYRHIHS+HHRLVVPYAFGALYNHPLEGLLLDT+GGAL+FL SGMSPRASIFFFS
Sbjct: 121 HHNKFLYRHIHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALAFLVSGMSPRASIFFFS 180

Query: 442 FATIKTVDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGTYM 263
           FAT+KTVDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYN+SQPFFV WDRILGTYM
Sbjct: 181 FATLKTVDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNFSQPFFVMWDRILGTYM 240

Query: 262 PYSLEKRTGGGFEAKPTKDCKDN 194
           PYSLEKRT GGFEA+PTKDCK+N
Sbjct: 241 PYSLEKRTNGGFEARPTKDCKEN 263


>ref|XP_012855270.1| PREDICTED: sphinganine C(4)-monooxygenase 1 [Erythranthe guttatus]
           gi|604302898|gb|EYU22423.1| hypothetical protein
           MIMGU_mgv1a012069mg [Erythranthe guttata]
          Length = 262

 Score =  454 bits (1169), Expect = e-125
 Identities = 211/261 (80%), Positives = 236/261 (90%)
 Frame = -2

Query: 976 IIKGGLEISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKDVVKG 797
           +I GGLE+SDE+LGT AP+VVYW+YSGIY++LGSLENYRLH+KKDEDEKNLVSKKDVVKG
Sbjct: 2   MINGGLEVSDELLGTVAPIVVYWLYSGIYVLLGSLENYRLHTKKDEDEKNLVSKKDVVKG 61

Query: 796 XXXXXXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHRYMHV 617
                      ATILFAVTG+D+++A +Q  S  VL RQ++VAM++LDTWQYFMHRYMH 
Sbjct: 62  VLLQQAVQAVVATILFAVTGSDTDSAQSQQTSFFVLARQLLVAMMVLDTWQYFMHRYMHH 121

Query: 616 NKFLYRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFFFSFA 437
           NKFLYRHIHS+HHRLVVPYAFGALYNHP+EGLLLDT+GGALSFL SGMSPRASIFFFSFA
Sbjct: 122 NKFLYRHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLISGMSPRASIFFFSFA 181

Query: 436 TIKTVDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGTYMPY 257
           T+KTVDDHCGLWLPGNLFHIFF NNSAYHDIHHQLYGNKYN+SQPFFV WDRILGTYMPY
Sbjct: 182 TLKTVDDHCGLWLPGNLFHIFFGNNSAYHDIHHQLYGNKYNFSQPFFVMWDRILGTYMPY 241

Query: 256 SLEKRTGGGFEAKPTKDCKDN 194
           SLEKR+ GGFEA+P KDCK+N
Sbjct: 242 SLEKRSKGGFEARPGKDCKEN 262


>ref|XP_004238795.1| PREDICTED: sphinganine C(4)-monooxygenase 1-like [Solanum
           lycopersicum]
          Length = 269

 Score =  436 bits (1122), Expect = e-119
 Identities = 203/259 (78%), Positives = 228/259 (88%)
 Frame = -2

Query: 970 KGGLEISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKDVVKGXX 791
           KGG  +SDE+LGTF P+VVYWVYSG+YLMLG+++NYRLHSKKDEDEKNLVSKK+VVKG  
Sbjct: 12  KGGY-VSDEVLGTFIPIVVYWVYSGLYLMLGNMDNYRLHSKKDEDEKNLVSKKEVVKGVL 70

Query: 790 XXXXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHRYMHVNK 611
                    AT+LFAVTG+D E+   Q AS+ VL RQ+ V ML+LDTWQYFMHRYMH NK
Sbjct: 71  LQQIVQAAVATVLFAVTGDDGESGGDQHASIFVLGRQLFVGMLMLDTWQYFMHRYMHQNK 130

Query: 610 FLYRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFFFSFATI 431
           FLY+HIH++HHRL+VPYAFGALYNHPLEGL+LDT+GGAL+FL SGMSPR SIFFFSFATI
Sbjct: 131 FLYKHIHAQHHRLIVPYAFGALYNHPLEGLILDTIGGALAFLVSGMSPRTSIFFFSFATI 190

Query: 430 KTVDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGTYMPYSL 251
           KTVDDHCGLWLPGNLFHIFF+NNSAYHDIHHQLYG KYNYSQPFFVTWDRILGTYMPY L
Sbjct: 191 KTVDDHCGLWLPGNLFHIFFKNNSAYHDIHHQLYGTKYNYSQPFFVTWDRILGTYMPYEL 250

Query: 250 EKRTGGGFEAKPTKDCKDN 194
           E+R  GGFEAKP KDCK++
Sbjct: 251 ERRPEGGFEAKPVKDCKEH 269


>gb|ABO93014.1| putative sterol desaturase [Solanum tuberosum]
          Length = 269

 Score =  436 bits (1121), Expect = e-119
 Identities = 202/259 (77%), Positives = 229/259 (88%)
 Frame = -2

Query: 970 KGGLEISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKDVVKGXX 791
           KGG  +SDE+LGTF P+VVYWVYSG+YLMLG+++NYRLHSKKDEDEKNLVSKK+VVKG  
Sbjct: 12  KGGY-VSDEILGTFLPIVVYWVYSGLYLMLGTMDNYRLHSKKDEDEKNLVSKKEVVKGVL 70

Query: 790 XXXXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHRYMHVNK 611
                    AT+LFAVTG+D E+   + AS+ VL RQ+ VAML+LDTWQYFMHRYMH NK
Sbjct: 71  LQQIVQAAVATVLFAVTGDDGESGGDRHASIFVLGRQLFVAMLMLDTWQYFMHRYMHQNK 130

Query: 610 FLYRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFFFSFATI 431
           FLY+HIH++HHRL++PYAFGALYNHPLEGL+LDT+GGAL+FL SGMSPR SIFFFSFATI
Sbjct: 131 FLYKHIHAQHHRLIIPYAFGALYNHPLEGLILDTIGGALAFLVSGMSPRTSIFFFSFATI 190

Query: 430 KTVDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGTYMPYSL 251
           KTVDDHCGLWLPGNLFHIFF+NNSAYHDIHHQLYG KYNYSQPFFVTWDRILGTYMPY L
Sbjct: 191 KTVDDHCGLWLPGNLFHIFFKNNSAYHDIHHQLYGTKYNYSQPFFVTWDRILGTYMPYEL 250

Query: 250 EKRTGGGFEAKPTKDCKDN 194
           E+R  GGFEAKP KDCK++
Sbjct: 251 ERRPEGGFEAKPVKDCKEH 269


>ref|XP_011075497.1| PREDICTED: sphinganine C(4)-monooxygenase 1-like [Sesamum indicum]
          Length = 265

 Score =  435 bits (1118), Expect = e-119
 Identities = 201/265 (75%), Positives = 231/265 (87%)
 Frame = -2

Query: 988 LNMQIIKGGLEISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKD 809
           + + I+ GGL++SDE+LGTFAP+V+YWVYSGIY+MLGS+E+YRLHS+KDEDEKNLV KKD
Sbjct: 1   MGILIMDGGLQVSDELLGTFAPIVLYWVYSGIYMMLGSMESYRLHSRKDEDEKNLVPKKD 60

Query: 808 VVKGXXXXXXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHR 629
           VV G           ATILFAVTG+D E+A AQ  SL+VL RQ  VAM++LDTWQYFMHR
Sbjct: 61  VVIGVLLQQAVQAIVATILFAVTGSDCESAEAQQISLVVLGRQFFVAMMVLDTWQYFMHR 120

Query: 628 YMHVNKFLYRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFF 449
           YMH NKFLYRH+HS+HHRLVVPYAFGALYNHPLEG+LLDTVGGAL+FL SGMSPRASIFF
Sbjct: 121 YMHHNKFLYRHVHSQHHRLVVPYAFGALYNHPLEGVLLDTVGGALAFLVSGMSPRASIFF 180

Query: 448 FSFATIKTVDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGT 269
           FSFAT+KTVDDHCGLWLPGN F +FF+NNSAYHDIHHQLYG KYNYSQPFFV WDRILGT
Sbjct: 181 FSFATMKTVDDHCGLWLPGNPFQLFFKNNSAYHDIHHQLYGTKYNYSQPFFVMWDRILGT 240

Query: 268 YMPYSLEKRTGGGFEAKPTKDCKDN 194
           Y+PY +EK+  GG +A+P KDCK+N
Sbjct: 241 YLPYKIEKKMEGGLQARPVKDCKEN 265


>ref|XP_006363216.1| PREDICTED: sphinganine C(4)-monooxygenase 1-like [Solanum
           tuberosum]
          Length = 269

 Score =  435 bits (1118), Expect = e-119
 Identities = 202/259 (77%), Positives = 228/259 (88%)
 Frame = -2

Query: 970 KGGLEISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKDVVKGXX 791
           KGG  +SDE+LGTF P+VVYWVYSG+YLMLG+++NYRLHSKKDEDEKNLVSKK+VVKG  
Sbjct: 12  KGGY-VSDEVLGTFLPIVVYWVYSGLYLMLGTMDNYRLHSKKDEDEKNLVSKKEVVKGVL 70

Query: 790 XXXXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHRYMHVNK 611
                    AT+LFAVTG+D E+   +  S+ VL RQ+ VAML+LDTWQYFMHRYMH NK
Sbjct: 71  LQQIVQAAVATVLFAVTGDDGESGGDRHTSIFVLGRQLFVAMLMLDTWQYFMHRYMHQNK 130

Query: 610 FLYRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFFFSFATI 431
           FLY+HIH++HHRL+VPYAFGALYNHPLEGL+LDT+GGAL+FL SGMSPR SIFFFSFATI
Sbjct: 131 FLYKHIHAQHHRLIVPYAFGALYNHPLEGLILDTIGGALAFLVSGMSPRTSIFFFSFATI 190

Query: 430 KTVDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGTYMPYSL 251
           KTVDDHCGLWLPGNLFHIFF+NNSAYHDIHHQLYG KYNYSQPFFVTWDRILGTYMPY L
Sbjct: 191 KTVDDHCGLWLPGNLFHIFFKNNSAYHDIHHQLYGTKYNYSQPFFVTWDRILGTYMPYEL 250

Query: 250 EKRTGGGFEAKPTKDCKDN 194
           E+R  GGFEAKP KDCK++
Sbjct: 251 ERRPEGGFEAKPVKDCKEH 269


>ref|XP_009793103.1| PREDICTED: sphinganine C(4)-monooxygenase 1-like [Nicotiana
           sylvestris]
          Length = 268

 Score =  433 bits (1113), Expect = e-118
 Identities = 200/260 (76%), Positives = 228/260 (87%), Gaps = 1/260 (0%)
 Frame = -2

Query: 970 KGGLE-ISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKDVVKGX 794
           +GG+  +SDE+LGT +P+VVYWVYSG+Y MLG +ENYRLH+KKDEDEKNLV+KK+VVKG 
Sbjct: 9   RGGIYGVSDELLGTISPIVVYWVYSGLYCMLGGMENYRLHTKKDEDEKNLVTKKEVVKGV 68

Query: 793 XXXXXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHRYMHVN 614
                     ATILFAVTGND E+   Q AS +VL RQ  V M++LDTWQYFMHRYMH N
Sbjct: 69  LLQQAVQAVVATILFAVTGNDGESGGNQQASFLVLARQFFVGMVVLDTWQYFMHRYMHQN 128

Query: 613 KFLYRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFFFSFAT 434
           KFLY+HIHS+HHRLVVPYAFGALYNHPLEGL+LDT+GGAL+FLFSGMSPRASIFFFSFAT
Sbjct: 129 KFLYKHIHSQHHRLVVPYAFGALYNHPLEGLILDTIGGALAFLFSGMSPRASIFFFSFAT 188

Query: 433 IKTVDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGTYMPYS 254
           IKTVDDHCGLWLPGNLFHI F+NNSAYHD+HHQLYG+KYN+SQPFFVTWDRILGTYMPY+
Sbjct: 189 IKTVDDHCGLWLPGNLFHIVFKNNSAYHDVHHQLYGSKYNFSQPFFVTWDRILGTYMPYA 248

Query: 253 LEKRTGGGFEAKPTKDCKDN 194
           L KR  GG+EA+P KDCKD+
Sbjct: 249 LVKRPEGGYEARPDKDCKDD 268


>ref|XP_006366702.1| PREDICTED: sphinganine C(4)-monooxygenase 1-like [Solanum
           tuberosum]
          Length = 266

 Score =  432 bits (1112), Expect = e-118
 Identities = 201/265 (75%), Positives = 232/265 (87%)
 Frame = -2

Query: 988 LNMQIIKGGLEISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKD 809
           ++  +++GGL +SDE+LGT  P+VVYWVYSG+Y +LG +ENYRLH+KKDEDEKNLV+KK+
Sbjct: 3   IDNDVLRGGLHVSDELLGTILPIVVYWVYSGLYCLLGGMENYRLHTKKDEDEKNLVTKKE 62

Query: 808 VVKGXXXXXXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHR 629
           VVKG           ATILFAVTGND ++   Q   L VLVRQ+ VAM+ILDTWQYFMHR
Sbjct: 63  VVKGVLLQQVVQSIVATILFAVTGNDGDSEGNQHGFL-VLVRQLFVAMVILDTWQYFMHR 121

Query: 628 YMHVNKFLYRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFF 449
           YMH NKFLY+HIHS+HHRLVVPYAFGALYNHPLEGLLLDT+GGAL+FLFSGMSPRASIFF
Sbjct: 122 YMHQNKFLYKHIHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALAFLFSGMSPRASIFF 181

Query: 448 FSFATIKTVDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGT 269
           FSFATIKTVDDHCGLWLPGNLFHI F+NNSAYHDIHHQLYG+KYN+SQPFFVTWDRILGT
Sbjct: 182 FSFATIKTVDDHCGLWLPGNLFHIVFKNNSAYHDIHHQLYGSKYNFSQPFFVTWDRILGT 241

Query: 268 YMPYSLEKRTGGGFEAKPTKDCKDN 194
           YMPY+L ++  GG+EA+P KDCKD+
Sbjct: 242 YMPYALVEKPEGGYEARPDKDCKDD 266


>ref|XP_009758683.1| PREDICTED: sphinganine C(4)-monooxygenase 1-like [Nicotiana
           sylvestris]
          Length = 272

 Score =  432 bits (1111), Expect = e-118
 Identities = 203/264 (76%), Positives = 228/264 (86%), Gaps = 1/264 (0%)
 Frame = -2

Query: 985 NMQIIKGGLEISDEMLGTFAPLVVYWVYSGIYLMLGS-LENYRLHSKKDEDEKNLVSKKD 809
           N Q+I GG  +SDE+LGTF P++VYWVYSG+Y MLG  ++NYRLHSKKDEDEKNLVSKK+
Sbjct: 9   NQQLI-GGYHVSDELLGTFLPIIVYWVYSGLYSMLGGVMDNYRLHSKKDEDEKNLVSKKE 67

Query: 808 VVKGXXXXXXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHR 629
           VVKG           AT+LFAVTGND E+   Q AS+ VL RQ  VAM++LDTWQYFMHR
Sbjct: 68  VVKGVLLQQVVQAAVATLLFAVTGNDGESDGDQHASIFVLGRQFFVAMVMLDTWQYFMHR 127

Query: 628 YMHVNKFLYRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFF 449
           YMH NKFLY+HIH++HHRL+VPYAFGALYNHPLEGL+LDT+GGAL+FL SGMSPR SIFF
Sbjct: 128 YMHQNKFLYKHIHAQHHRLIVPYAFGALYNHPLEGLILDTIGGALAFLVSGMSPRTSIFF 187

Query: 448 FSFATIKTVDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGT 269
           FSFATIKTVDDHCGLWLPGNLFHIFF+NNSAYHDIHHQLYG K+NYSQPFFVTWDRILGT
Sbjct: 188 FSFATIKTVDDHCGLWLPGNLFHIFFKNNSAYHDIHHQLYGTKFNYSQPFFVTWDRILGT 247

Query: 268 YMPYSLEKRTGGGFEAKPTKDCKD 197
           YMPY LEKR  GGFEA+P KDCK+
Sbjct: 248 YMPYELEKRPEGGFEARPVKDCKE 271


>ref|XP_009626041.1| PREDICTED: sphinganine C(4)-monooxygenase 1-like [Nicotiana
           tomentosiformis]
          Length = 268

 Score =  432 bits (1111), Expect = e-118
 Identities = 200/259 (77%), Positives = 227/259 (87%), Gaps = 1/259 (0%)
 Frame = -2

Query: 967 GGLE-ISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKDVVKGXX 791
           GGL  +SDE+LGT +P+VVYWVYSG+Y MLG +ENYRLH+KKDEDEKNLV+KK+VVKG  
Sbjct: 10  GGLYGVSDELLGTISPIVVYWVYSGLYCMLGGMENYRLHTKKDEDEKNLVTKKEVVKGVL 69

Query: 790 XXXXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHRYMHVNK 611
                    AT+LFAVTGND E+   Q A ++VL RQ  VAM++LDTWQYFMHRYMH NK
Sbjct: 70  LQQAVQAVVATVLFAVTGNDGESGGNQQAGVLVLARQFFVAMVVLDTWQYFMHRYMHQNK 129

Query: 610 FLYRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFFFSFATI 431
           FLY+HIHS+HHRLVVPYAFGALYNHPLEGL+LDT+GGAL+FLFSGMSPRASIFFFSFATI
Sbjct: 130 FLYKHIHSQHHRLVVPYAFGALYNHPLEGLILDTIGGALAFLFSGMSPRASIFFFSFATI 189

Query: 430 KTVDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGTYMPYSL 251
           KTVDDHCGLWLPGNLFHI F+NNSAYHD+HHQLYG+KYN+SQPFFVTWDRILGTYMPY+L
Sbjct: 190 KTVDDHCGLWLPGNLFHIVFKNNSAYHDVHHQLYGSKYNFSQPFFVTWDRILGTYMPYAL 249

Query: 250 EKRTGGGFEAKPTKDCKDN 194
            KR  GG+EAKP  DCKD+
Sbjct: 250 VKRPEGGYEAKPDNDCKDD 268


>ref|XP_004228378.1| PREDICTED: sphinganine C(4)-monooxygenase 1-like [Solanum
           lycopersicum]
          Length = 266

 Score =  432 bits (1111), Expect = e-118
 Identities = 200/265 (75%), Positives = 232/265 (87%)
 Frame = -2

Query: 988 LNMQIIKGGLEISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKD 809
           ++  +++GGL +SDE+LGT  P+VVYWVYSG+Y +LG +ENYRLH+KKDEDEKNLV+KK+
Sbjct: 3   IDNDVVRGGLHVSDELLGTILPIVVYWVYSGLYCLLGGMENYRLHTKKDEDEKNLVTKKE 62

Query: 808 VVKGXXXXXXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHR 629
           VVKG           AT+LFAVTGND ++   Q   L VLVRQ+ +AM+ILDTWQYFMHR
Sbjct: 63  VVKGVLLQQVVQSVVATVLFAVTGNDGDSDGNQHGFL-VLVRQLFLAMVILDTWQYFMHR 121

Query: 628 YMHVNKFLYRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFF 449
           YMH NKFLY+HIHS+HHRLVVPYAFGALYNHPLEGLLLDT+GGAL+FLFSGMSPRASIFF
Sbjct: 122 YMHQNKFLYKHIHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALAFLFSGMSPRASIFF 181

Query: 448 FSFATIKTVDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGT 269
           FSFATIKTVDDHCGLWLPGNLFHI F+NNSAYHDIHHQLYG+KYN+SQPFFVTWDRILGT
Sbjct: 182 FSFATIKTVDDHCGLWLPGNLFHIVFKNNSAYHDIHHQLYGSKYNFSQPFFVTWDRILGT 241

Query: 268 YMPYSLEKRTGGGFEAKPTKDCKDN 194
           YMPY+L +R  GG+EA+P KDCKD+
Sbjct: 242 YMPYALVERPEGGYEARPDKDCKDD 266


>gb|ABO92998.1| putative sterol desaturase [Solanum tuberosum]
          Length = 269

 Score =  432 bits (1111), Expect = e-118
 Identities = 200/259 (77%), Positives = 227/259 (87%)
 Frame = -2

Query: 970 KGGLEISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKDVVKGXX 791
           KGG  +SDE+LGTF P+VVYWVYSG+YLMLG+++NYRLHSKKDEDEKNLVSKK+VVKG  
Sbjct: 12  KGGY-VSDEVLGTFLPIVVYWVYSGLYLMLGNMDNYRLHSKKDEDEKNLVSKKEVVKGVL 70

Query: 790 XXXXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHRYMHVNK 611
                    AT+LFAVTG+D E+   +  S+ VL RQ+ +AML+LDTWQYFMHRYMH NK
Sbjct: 71  LQQIVQAAVATVLFAVTGDDGESGGDRHTSIFVLGRQLFIAMLMLDTWQYFMHRYMHQNK 130

Query: 610 FLYRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFFFSFATI 431
           FLY+HIH++HHRL+VPYAFGALYNHPLEGL+LDT+GGAL+FL SGMSPR SIFFFSFATI
Sbjct: 131 FLYKHIHAQHHRLIVPYAFGALYNHPLEGLILDTIGGALAFLVSGMSPRTSIFFFSFATI 190

Query: 430 KTVDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGTYMPYSL 251
           KTVDDHCGLWLPGNLFHIFF+NNSAYHDIHHQLYG KYNYSQPFFVTWD ILGTYMPY L
Sbjct: 191 KTVDDHCGLWLPGNLFHIFFKNNSAYHDIHHQLYGTKYNYSQPFFVTWDMILGTYMPYEL 250

Query: 250 EKRTGGGFEAKPTKDCKDN 194
           E+R  GGFEAKP KDCK++
Sbjct: 251 ERRPEGGFEAKPVKDCKEH 269


>emb|CDP00325.1| unnamed protein product [Coffea canephora]
          Length = 261

 Score =  432 bits (1110), Expect = e-118
 Identities = 199/257 (77%), Positives = 225/257 (87%)
 Frame = -2

Query: 964 GLEISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKDVVKGXXXX 785
           GLEISDE++GTF P+V+YWVYSGIY+MLG ++NYRLHS+KDEDEKNLV+KKDVVKG    
Sbjct: 5   GLEISDELMGTFVPIVMYWVYSGIYMMLGGMDNYRLHSRKDEDEKNLVAKKDVVKGVLLQ 64

Query: 784 XXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHRYMHVNKFL 605
                  AT+LFA TGND EA   Q   L+ L +Q  +AM+ LDTWQYFMHRYMH NKFL
Sbjct: 65  QAVQAVVATLLFAATGNDGEAEGDQQRPLVDLAKQFFIAMVALDTWQYFMHRYMHQNKFL 124

Query: 604 YRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFFFSFATIKT 425
           Y+HIHS+HHRLVVPYA+GALYNHPLEGL+LDTVGGAL+FL SGMSPRASIFFFSFAT+KT
Sbjct: 125 YKHIHSQHHRLVVPYAYGALYNHPLEGLILDTVGGALAFLLSGMSPRASIFFFSFATMKT 184

Query: 424 VDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGTYMPYSLEK 245
           VDDHCGLWLPGNLFHIFFRNNSAYHDIHHQL+G KYN+SQPFFVTWDRILGTYMPY+LEK
Sbjct: 185 VDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLHGTKYNFSQPFFVTWDRILGTYMPYTLEK 244

Query: 244 RTGGGFEAKPTKDCKDN 194
           R  GGFEA+PTKD K++
Sbjct: 245 RVDGGFEARPTKDYKED 261


>ref|XP_002275229.1| PREDICTED: sphinganine C(4)-monooxygenase 1 [Vitis vinifera]
           gi|296086379|emb|CBI31968.3| unnamed protein product
           [Vitis vinifera]
          Length = 258

 Score =  432 bits (1110), Expect = e-118
 Identities = 202/257 (78%), Positives = 222/257 (86%)
 Frame = -2

Query: 964 GLEISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKDVVKGXXXX 785
           G E+SDE+LG F P++VYWVYSGIY+ LGSLENYRLHSKK+E++KNLVSK  VVKG    
Sbjct: 2   GFEVSDELLGAFVPILVYWVYSGIYIPLGSLENYRLHSKKEEEDKNLVSKGTVVKGVILQ 61

Query: 784 XXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHRYMHVNKFL 605
                  A +LF VTG+DS AA     SLIVL RQ  +AM +LDTWQYFMHRYMH NKFL
Sbjct: 62  QVIQAVVAILLFTVTGDDSGAAAGPKPSLIVLARQFFIAMFVLDTWQYFMHRYMHHNKFL 121

Query: 604 YRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFFFSFATIKT 425
           YRHIHS+HHRLVVPYAFGALYNHPLEGLLLDT+GGALSFL SGMSPRASIFFFSFATIKT
Sbjct: 122 YRHIHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLLSGMSPRASIFFFSFATIKT 181

Query: 424 VDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGTYMPYSLEK 245
           VDDHCGLWLPGNLFH+FFRNNSAYHDIHHQLYG+KYN+SQPFFV WD+ILGTYMPYSLEK
Sbjct: 182 VDDHCGLWLPGNLFHLFFRNNSAYHDIHHQLYGSKYNFSQPFFVMWDKILGTYMPYSLEK 241

Query: 244 RTGGGFEAKPTKDCKDN 194
           R GGG EA+PTK+ KDN
Sbjct: 242 RAGGGLEARPTKEFKDN 258


>ref|XP_012067630.1| PREDICTED: sphinganine C(4)-monooxygenase 1 [Jatropha curcas]
          Length = 263

 Score =  431 bits (1108), Expect = e-118
 Identities = 196/257 (76%), Positives = 224/257 (87%)
 Frame = -2

Query: 964 GLEISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKDVVKGXXXX 785
           G +ISDE+LGTFAP+VVYWVYSG+Y++LG  +NYRLH+K+DEDEKNLVSK  V KG    
Sbjct: 7   GFQISDELLGTFAPIVVYWVYSGLYILLGFFDNYRLHTKQDEDEKNLVSKSTVFKGVLLQ 66

Query: 784 XXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHRYMHVNKFL 605
                  A +LF VTGNDS+A      SLIVL RQ + AML++DTWQYFMHRYMH NKFL
Sbjct: 67  QSIQAVVAILLFTVTGNDSQADTYPQPSLIVLARQFVTAMLVMDTWQYFMHRYMHHNKFL 126

Query: 604 YRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFFFSFATIKT 425
           Y+HIHS+HHRLVVPYAFGALYNHPLEGLLLDT+GGALSFL SGMSPR+SIFFFSFATIKT
Sbjct: 127 YKHIHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLMSGMSPRSSIFFFSFATIKT 186

Query: 424 VDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGTYMPYSLEK 245
           VDDHCGLWLPGNLFH+FF+NN+AYHD+HHQLYG+KYN+SQPFFV WDRILGTYMPYSLEK
Sbjct: 187 VDDHCGLWLPGNLFHVFFKNNTAYHDVHHQLYGSKYNFSQPFFVMWDRILGTYMPYSLEK 246

Query: 244 RTGGGFEAKPTKDCKDN 194
           + GGGFE +PTK+CKD+
Sbjct: 247 KEGGGFEVRPTKECKDD 263


>gb|KDP41189.1| hypothetical protein JCGZ_15596 [Jatropha curcas]
          Length = 258

 Score =  431 bits (1108), Expect = e-118
 Identities = 196/257 (76%), Positives = 224/257 (87%)
 Frame = -2

Query: 964 GLEISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKDVVKGXXXX 785
           G +ISDE+LGTFAP+VVYWVYSG+Y++LG  +NYRLH+K+DEDEKNLVSK  V KG    
Sbjct: 2   GFQISDELLGTFAPIVVYWVYSGLYILLGFFDNYRLHTKQDEDEKNLVSKSTVFKGVLLQ 61

Query: 784 XXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHRYMHVNKFL 605
                  A +LF VTGNDS+A      SLIVL RQ + AML++DTWQYFMHRYMH NKFL
Sbjct: 62  QSIQAVVAILLFTVTGNDSQADTYPQPSLIVLARQFVTAMLVMDTWQYFMHRYMHHNKFL 121

Query: 604 YRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFFFSFATIKT 425
           Y+HIHS+HHRLVVPYAFGALYNHPLEGLLLDT+GGALSFL SGMSPR+SIFFFSFATIKT
Sbjct: 122 YKHIHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLMSGMSPRSSIFFFSFATIKT 181

Query: 424 VDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGTYMPYSLEK 245
           VDDHCGLWLPGNLFH+FF+NN+AYHD+HHQLYG+KYN+SQPFFV WDRILGTYMPYSLEK
Sbjct: 182 VDDHCGLWLPGNLFHVFFKNNTAYHDVHHQLYGSKYNFSQPFFVMWDRILGTYMPYSLEK 241

Query: 244 RTGGGFEAKPTKDCKDN 194
           + GGGFE +PTK+CKD+
Sbjct: 242 KEGGGFEVRPTKECKDD 258


>gb|EPS64083.1| hypothetical protein M569_10698 [Genlisea aurea]
          Length = 266

 Score =  431 bits (1108), Expect = e-118
 Identities = 203/266 (76%), Positives = 227/266 (85%), Gaps = 3/266 (1%)
 Frame = -2

Query: 982 MQIIKGGLEISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKDVV 803
           M II GG E+SDE LGTF P++VYW+YSGIY++LGSLENYRLH+KKDEDEKN V KKDVV
Sbjct: 1   MDIIIGGYEVSDEFLGTFVPIIVYWIYSGIYVLLGSLENYRLHTKKDEDEKNSVPKKDVV 60

Query: 802 KGXXXXXXXXXXXATILFAVTGNDSEAALAQPA---SLIVLVRQIIVAMLILDTWQYFMH 632
           KG           ATILFAVT  + + ++  P+   SLIVL RQ  VAM++LDTWQYFMH
Sbjct: 61  KGVLLQQAVQAIVATILFAVTAENRDESVEPPSENNSLIVLARQFFVAMVVLDTWQYFMH 120

Query: 631 RYMHVNKFLYRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIF 452
           RYMH NKFLYRHIHS+HHRL+VPYAFGALYNHPLEGL+LDTVGG+LSFL SGMSPRASIF
Sbjct: 121 RYMHHNKFLYRHIHSQHHRLLVPYAFGALYNHPLEGLILDTVGGSLSFLVSGMSPRASIF 180

Query: 451 FFSFATIKTVDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILG 272
           FFSFAT+KTVDDHCGLWLPGN FH FF NNSAYHDIHHQLYGNKYN+SQPFFVTWD+ILG
Sbjct: 181 FFSFATLKTVDDHCGLWLPGNAFHFFFSNNSAYHDIHHQLYGNKYNFSQPFFVTWDKILG 240

Query: 271 TYMPYSLEKRTGGGFEAKPTKDCKDN 194
           TYMPYSLEK++ GGF AKP KD K+N
Sbjct: 241 TYMPYSLEKKSNGGFIAKPAKDDKEN 266


>ref|XP_009596162.1| PREDICTED: sphinganine C(4)-monooxygenase 1 [Nicotiana
           tomentosiformis]
          Length = 272

 Score =  428 bits (1100), Expect = e-117
 Identities = 201/265 (75%), Positives = 228/265 (86%), Gaps = 1/265 (0%)
 Frame = -2

Query: 985 NMQIIKGGLEISDEMLGTFAPLVVYWVYSGIYLMLGSL-ENYRLHSKKDEDEKNLVSKKD 809
           N Q+I GG  +SDE+LGTF P++VYWVYSG+Y MLG + +NYRLHSKKDEDEKNLV+KK+
Sbjct: 9   NQQLI-GGYHVSDELLGTFLPIIVYWVYSGLYSMLGGIMDNYRLHSKKDEDEKNLVAKKE 67

Query: 808 VVKGXXXXXXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHR 629
           VVKG           AT+LFAVTGND E+   Q AS+ VL RQ  VAM++LDTWQYFMHR
Sbjct: 68  VVKGVLLQQVVQAAVATLLFAVTGNDGESDGDQHASIFVLGRQFFVAMVMLDTWQYFMHR 127

Query: 628 YMHVNKFLYRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFF 449
           YMH NKFLY+HIH++HHRL+VPYAFGALYNHPLEGL+LDT+GGAL+FL SGMSPR SIFF
Sbjct: 128 YMHQNKFLYKHIHAQHHRLIVPYAFGALYNHPLEGLILDTIGGALAFLVSGMSPRTSIFF 187

Query: 448 FSFATIKTVDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGT 269
           FSFATIKTVDDHCGLWLP NLFHIFF+NNSAYHDIHHQLYG K+NYSQPFFVTWDRILGT
Sbjct: 188 FSFATIKTVDDHCGLWLPVNLFHIFFKNNSAYHDIHHQLYGTKFNYSQPFFVTWDRILGT 247

Query: 268 YMPYSLEKRTGGGFEAKPTKDCKDN 194
           YMPY LEKR  GGFEA+P KDCK++
Sbjct: 248 YMPYELEKRPEGGFEARPVKDCKEH 272


>ref|XP_007046113.1| Sphingoid base hydroxylase 2 [Theobroma cacao]
           gi|508710048|gb|EOY01945.1| Sphingoid base hydroxylase 2
           [Theobroma cacao]
          Length = 258

 Score =  428 bits (1100), Expect = e-117
 Identities = 198/257 (77%), Positives = 223/257 (86%)
 Frame = -2

Query: 964 GLEISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKDVVKGXXXX 785
           G  +SDE+LGTF P+VVYW+YSGIY+ LGS E+YRLHSKKDEDEKNLVSK+ VVKG    
Sbjct: 2   GFTVSDELLGTFVPIVVYWIYSGIYMCLGSFEDYRLHSKKDEDEKNLVSKQTVVKGVLLQ 61

Query: 784 XXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHRYMHVNKFL 605
                  A +LF VTG+D+ A+  QP+SLIVL RQ + AML++DTWQYF+HRY H NKFL
Sbjct: 62  QTVQAVVAILLFTVTGSDAGASAGQPSSLIVLARQFVTAMLVMDTWQYFLHRYFHHNKFL 121

Query: 604 YRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFFFSFATIKT 425
           YRH+HS+HHRLVVPYAFGALYNHPLEGLLLDTVGGALSFL SGMSPR SIFFFSFATIKT
Sbjct: 122 YRHLHSQHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLLSGMSPRTSIFFFSFATIKT 181

Query: 424 VDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGTYMPYSLEK 245
           VDDHCGL LPGNLFHIFFRNNSAYHD+HHQLYG+KYN+SQPFF+ WDRILGTYMPYSLEK
Sbjct: 182 VDDHCGLLLPGNLFHIFFRNNSAYHDVHHQLYGSKYNFSQPFFIMWDRILGTYMPYSLEK 241

Query: 244 RTGGGFEAKPTKDCKDN 194
           R  GGFEA+PTK+ KD+
Sbjct: 242 RADGGFEARPTKEYKDD 258


>ref|XP_010089753.1| Sphingoid base hydroxylase 2 [Morus notabilis]
           gi|587848030|gb|EXB38329.1| Sphingoid base hydroxylase 2
           [Morus notabilis]
          Length = 258

 Score =  427 bits (1098), Expect = e-117
 Identities = 199/256 (77%), Positives = 218/256 (85%)
 Frame = -2

Query: 964 GLEISDEMLGTFAPLVVYWVYSGIYLMLGSLENYRLHSKKDEDEKNLVSKKDVVKGXXXX 785
           G  +SDE+LGTFAP+V+YWVYSGIY +L S ENYRLHSK DEDEKNLVSK+ VVKG    
Sbjct: 2   GFAVSDEVLGTFAPIVIYWVYSGIYALLDSFENYRLHSKIDEDEKNLVSKRTVVKGVLLQ 61

Query: 784 XXXXXXXATILFAVTGNDSEAALAQPASLIVLVRQIIVAMLILDTWQYFMHRYMHVNKFL 605
                  A +LF VTGNDS  A     SLIVL RQ + AML+LDTWQYFMHRYMH NKFL
Sbjct: 62  QTIQAVVAILLFTVTGNDSGDAAGPKPSLIVLARQFVTAMLVLDTWQYFMHRYMHHNKFL 121

Query: 604 YRHIHSRHHRLVVPYAFGALYNHPLEGLLLDTVGGALSFLFSGMSPRASIFFFSFATIKT 425
           Y+HIHS+HHRLVVPYAFGALYNHPLEGLLLDT+GGALSFL SGMSPRASIFFFSFAT+KT
Sbjct: 122 YKHIHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLLSGMSPRASIFFFSFATVKT 181

Query: 424 VDDHCGLWLPGNLFHIFFRNNSAYHDIHHQLYGNKYNYSQPFFVTWDRILGTYMPYSLEK 245
           VDDHCGLWLPGNLFH FFRNN+AYHD+HHQLYG+KYN+SQPFFV WDRILGTYMPYSL+K
Sbjct: 182 VDDHCGLWLPGNLFHFFFRNNTAYHDVHHQLYGSKYNFSQPFFVMWDRILGTYMPYSLDK 241

Query: 244 RTGGGFEAKPTKDCKD 197
           R GGGFE +P KD KD
Sbjct: 242 RAGGGFETRPLKDYKD 257