BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Forsythia22_contig00001059
         (3243 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011089331.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like ...  1267   0.0  
ref|XP_011089332.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like ...  1259   0.0  
ref|XP_011089334.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isofo...  1258   0.0  
ref|XP_011089333.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like ...  1256   0.0  
emb|CDP07144.1| unnamed protein product [Coffea canephora]           1230   0.0  
ref|XP_011089337.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isofo...  1182   0.0  
ref|XP_011089338.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isofo...  1169   0.0  
ref|XP_009787730.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isofo...  1144   0.0  
ref|XP_009787729.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isofo...  1144   0.0  
ref|XP_009787726.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isofo...  1144   0.0  
ref|XP_009617749.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isofo...  1142   0.0  
ref|XP_009617748.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isofo...  1142   0.0  
ref|XP_009617745.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isofo...  1142   0.0  
ref|XP_010651134.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isofo...  1137   0.0  
ref|XP_010651133.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isofo...  1137   0.0  
ref|XP_009601717.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like ...  1135   0.0  
ref|XP_002526365.1| sumo ligase, putative [Ricinus communis] gi|...  1134   0.0  
ref|XP_009601705.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like ...  1130   0.0  
ref|XP_006340142.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like ...  1127   0.0  
ref|XP_006340140.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like ...  1127   0.0  

>ref|XP_011089331.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like isoform X1 [Sesamum
            indicum]
          Length = 878

 Score = 1267 bits (3279), Expect = 0.0
 Identities = 616/797 (77%), Positives = 699/797 (87%), Gaps = 7/797 (0%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLV++CKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSD+RVSG+WAKKNAV
Sbjct: 1    MDLVSNCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDERVSGLWAKKNAV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLCGSTL 2807
            GKE+VAKLVDDTYRKMQ+SGA+DLASKS + +   N++PKEETE+ +QM+KIRCLCGSTL
Sbjct: 61   GKEDVAKLVDDTYRKMQVSGASDLASKSQTVSDNSNIRPKEETEDRHQMEKIRCLCGSTL 120

Query: 2806 PTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEIFYCEICRLSRADPFWMTVAHP 2627
            PTDSMIKCEDPRCNVWQH+ACV+IPEKP EG+LPN P+IFYCE+CRLSRADPFW+TVAHP
Sbjct: 121  PTDSMIKCEDPRCNVWQHMACVLIPEKPTEGILPNPPDIFYCEVCRLSRADPFWVTVAHP 180

Query: 2626 LYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQW 2447
            LYPVKL IT+VP+DGSNP QSIEKTF L RAD+DLLSKQEYDVQAWCMLLNDKV FRMQW
Sbjct: 181  LYPVKLNITNVPADGSNPSQSIEKTFSLARADRDLLSKQEYDVQAWCMLLNDKVTFRMQW 240

Query: 2446 PLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLGV 2267
            P YADLQ+NGVPVRAINRPGSQ LGANGRDDGP ITPCTRDG+NKI + GCDARIFC+GV
Sbjct: 241  PQYADLQVNGVPVRAINRPGSQLLGANGRDDGPVITPCTRDGLNKIFVGGCDARIFCMGV 300

Query: 2266 RIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPVN 2087
            RI KRR+L QVLN+IPKE EGERFEDALA VRRCVGGGAATENADSDSDIEVVADFIPVN
Sbjct: 301  RIAKRRSLQQVLNMIPKEDEGERFEDALAPVRRCVGGGAATENADSDSDIEVVADFIPVN 360

Query: 2086 LRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPYF 1907
            LRCPMSGLRMKVAGRF+ C HMGCFDLEVFVEMN RSRKWQCPICLKNYSLEKIIIDPYF
Sbjct: 361  LRCPMSGLRMKVAGRFKHCAHMGCFDLEVFVEMNQRSRKWQCPICLKNYSLEKIIIDPYF 420

Query: 1906 NRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEGESKPK 1727
            NRITSK+RNCGEDV EIEVKPDGSWRAK EGDR+ +GELGLWHLPDG++C S + +SKPK
Sbjct: 421  NRITSKMRNCGEDVAEIEVKPDGSWRAKAEGDRKGVGELGLWHLPDGSICASADEDSKPK 480

Query: 1726 QEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFENDDHNVI 1547
             E +K IKQE GSDS+AGLKLG+K+NRNGCWE +K D++  +++A+R  E+FE +  N+I
Sbjct: 481  LE-LKPIKQEAGSDSNAGLKLGIKKNRNGCWEFHKPDNMQGITTANRFDENFEKNGQNII 539

Query: 1546 PMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQNLPAPL-- 1373
            PMSSSATGSG+D ED SVNQ G  NLDF+T NGIE ESI LN+D ++GF+++   AP   
Sbjct: 540  PMSSSATGSGKDCEDGSVNQVGGGNLDFSTVNGIEYESIPLNIDPTHGFSDRTTSAPAGE 599

Query: 1372 EEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGN----GI 1205
             EVI+LSDSEEE EPL+SS A YKN   D GG  FP +QHGIP SY E PA GN     +
Sbjct: 600  AEVIVLSDSEEEIEPLMSSEAGYKNTGPDTGGVPFPAAQHGIPDSYYEHPALGNVGSSCL 659

Query: 1204 DLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGYTLT 1025
             L+N +DDDFG++MW+LPSGS G PGFQLFGSD DV++ALV++QHGS+NC +++NGY LT
Sbjct: 660  GLYNTNDDDFGVNMWTLPSGSHGGPGFQLFGSDVDVSEALVEMQHGSLNCSSSINGYALT 719

Query: 1024 AETAMGSAALVPESSVKHTNVNDGLVDNPLAFSGNDPSLQIFLPTRPSDAS-AQADMRDL 848
            AETAMGSAALV ESS + +N NDGLVDNPLAFSGNDPSLQIFLPTRPSDAS A +D+RD 
Sbjct: 720  AETAMGSAALVSESSAQRSNTNDGLVDNPLAFSGNDPSLQIFLPTRPSDASAAPSDLRDH 779

Query: 847  PDVSNGIRXVEGVMVSL 797
            PDVSNGIR  + + + L
Sbjct: 780  PDVSNGIRAKDWISLRL 796



 Score = 95.5 bits (236), Expect = 2e-16
 Identities = 50/78 (64%), Positives = 56/78 (71%)
 Frame = -2

Query: 818  GGGHGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXGMNDNKSGKASRERSDSPFSFPR 639
            G G GE AA++ ++S  QLQSK               GMNDN+SGK SRERSDSPF+FPR
Sbjct: 801  GAGQGESAAASGINSAQQLQSKDDTLNSLADNASLLLGMNDNRSGKTSRERSDSPFTFPR 860

Query: 638  QRRSVRPRLYLSIDSDSE 585
            QRRSVRPRLYLSIDSDSE
Sbjct: 861  QRRSVRPRLYLSIDSDSE 878


>ref|XP_011089332.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like isoform X2 [Sesamum
            indicum]
          Length = 876

 Score = 1259 bits (3258), Expect = 0.0
 Identities = 614/797 (77%), Positives = 697/797 (87%), Gaps = 7/797 (0%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLV++CKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSD+R  G+WAKKNAV
Sbjct: 1    MDLVSNCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDER--GLWAKKNAV 58

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLCGSTL 2807
            GKE+VAKLVDDTYRKMQ+SGA+DLASKS + +   N++PKEETE+ +QM+KIRCLCGSTL
Sbjct: 59   GKEDVAKLVDDTYRKMQVSGASDLASKSQTVSDNSNIRPKEETEDRHQMEKIRCLCGSTL 118

Query: 2806 PTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEIFYCEICRLSRADPFWMTVAHP 2627
            PTDSMIKCEDPRCNVWQH+ACV+IPEKP EG+LPN P+IFYCE+CRLSRADPFW+TVAHP
Sbjct: 119  PTDSMIKCEDPRCNVWQHMACVLIPEKPTEGILPNPPDIFYCEVCRLSRADPFWVTVAHP 178

Query: 2626 LYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQW 2447
            LYPVKL IT+VP+DGSNP QSIEKTF L RAD+DLLSKQEYDVQAWCMLLNDKV FRMQW
Sbjct: 179  LYPVKLNITNVPADGSNPSQSIEKTFSLARADRDLLSKQEYDVQAWCMLLNDKVTFRMQW 238

Query: 2446 PLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLGV 2267
            P YADLQ+NGVPVRAINRPGSQ LGANGRDDGP ITPCTRDG+NKI + GCDARIFC+GV
Sbjct: 239  PQYADLQVNGVPVRAINRPGSQLLGANGRDDGPVITPCTRDGLNKIFVGGCDARIFCMGV 298

Query: 2266 RIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPVN 2087
            RI KRR+L QVLN+IPKE EGERFEDALA VRRCVGGGAATENADSDSDIEVVADFIPVN
Sbjct: 299  RIAKRRSLQQVLNMIPKEDEGERFEDALAPVRRCVGGGAATENADSDSDIEVVADFIPVN 358

Query: 2086 LRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPYF 1907
            LRCPMSGLRMKVAGRF+ C HMGCFDLEVFVEMN RSRKWQCPICLKNYSLEKIIIDPYF
Sbjct: 359  LRCPMSGLRMKVAGRFKHCAHMGCFDLEVFVEMNQRSRKWQCPICLKNYSLEKIIIDPYF 418

Query: 1906 NRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEGESKPK 1727
            NRITSK+RNCGEDV EIEVKPDGSWRAK EGDR+ +GELGLWHLPDG++C S + +SKPK
Sbjct: 419  NRITSKMRNCGEDVAEIEVKPDGSWRAKAEGDRKGVGELGLWHLPDGSICASADEDSKPK 478

Query: 1726 QEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFENDDHNVI 1547
             E +K IKQE GSDS+AGLKLG+K+NRNGCWE +K D++  +++A+R  E+FE +  N+I
Sbjct: 479  LE-LKPIKQEAGSDSNAGLKLGIKKNRNGCWEFHKPDNMQGITTANRFDENFEKNGQNII 537

Query: 1546 PMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQNLPAPL-- 1373
            PMSSSATGSG+D ED SVNQ G  NLDF+T NGIE ESI LN+D ++GF+++   AP   
Sbjct: 538  PMSSSATGSGKDCEDGSVNQVGGGNLDFSTVNGIEYESIPLNIDPTHGFSDRTTSAPAGE 597

Query: 1372 EEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGN----GI 1205
             EVI+LSDSEEE EPL+SS A YKN   D GG  FP +QHGIP SY E PA GN     +
Sbjct: 598  AEVIVLSDSEEEIEPLMSSEAGYKNTGPDTGGVPFPAAQHGIPDSYYEHPALGNVGSSCL 657

Query: 1204 DLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGYTLT 1025
             L+N +DDDFG++MW+LPSGS G PGFQLFGSD DV++ALV++QHGS+NC +++NGY LT
Sbjct: 658  GLYNTNDDDFGVNMWTLPSGSHGGPGFQLFGSDVDVSEALVEMQHGSLNCSSSINGYALT 717

Query: 1024 AETAMGSAALVPESSVKHTNVNDGLVDNPLAFSGNDPSLQIFLPTRPSDAS-AQADMRDL 848
            AETAMGSAALV ESS + +N NDGLVDNPLAFSGNDPSLQIFLPTRPSDAS A +D+RD 
Sbjct: 718  AETAMGSAALVSESSAQRSNTNDGLVDNPLAFSGNDPSLQIFLPTRPSDASAAPSDLRDH 777

Query: 847  PDVSNGIRXVEGVMVSL 797
            PDVSNGIR  + + + L
Sbjct: 778  PDVSNGIRAKDWISLRL 794



 Score = 95.5 bits (236), Expect = 2e-16
 Identities = 50/78 (64%), Positives = 56/78 (71%)
 Frame = -2

Query: 818  GGGHGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXGMNDNKSGKASRERSDSPFSFPR 639
            G G GE AA++ ++S  QLQSK               GMNDN+SGK SRERSDSPF+FPR
Sbjct: 799  GAGQGESAAASGINSAQQLQSKDDTLNSLADNASLLLGMNDNRSGKTSRERSDSPFTFPR 858

Query: 638  QRRSVRPRLYLSIDSDSE 585
            QRRSVRPRLYLSIDSDSE
Sbjct: 859  QRRSVRPRLYLSIDSDSE 876


>ref|XP_011089334.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isoform X1 [Sesamum indicum]
            gi|747083900|ref|XP_011089336.1| PREDICTED: E3
            SUMO-protein ligase SIZ1 isoform X1 [Sesamum indicum]
          Length = 877

 Score = 1258 bits (3255), Expect = 0.0
 Identities = 620/797 (77%), Positives = 694/797 (87%), Gaps = 7/797 (0%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLV SCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSD+RVSG+WAKKNAV
Sbjct: 1    MDLVASCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDERVSGLWAKKNAV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLCGSTL 2807
            GKE+VAKLVDDTYRKMQ+SGA DLASKS   +   N+K KEE E+ YQM+KIRC+CGSTL
Sbjct: 61   GKEDVAKLVDDTYRKMQVSGAADLASKSQVVSDSTNIKCKEEAEDCYQMEKIRCVCGSTL 120

Query: 2806 PTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEIFYCEICRLSRADPFWMTVAHP 2627
            PTDSMIKCEDPRCNVWQH+ACV+IPEKP EGVLPN P+IFYCE+CRLSRADPFW+TVAHP
Sbjct: 121  PTDSMIKCEDPRCNVWQHMACVLIPEKPTEGVLPNPPDIFYCEVCRLSRADPFWVTVAHP 180

Query: 2626 LYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQW 2447
            LYPVKL IT+VP+DGSNP QSIEKTF LTRAD+DLLSKQEYDVQAWCMLLNDKV FRMQW
Sbjct: 181  LYPVKLNITNVPADGSNPSQSIEKTFHLTRADRDLLSKQEYDVQAWCMLLNDKVTFRMQW 240

Query: 2446 PLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLGV 2267
            P YADLQ+NGVPVRAINRPGSQ LGANGRDDGP ITPCTRDGINKI L GCDAR+FC+GV
Sbjct: 241  PQYADLQVNGVPVRAINRPGSQLLGANGRDDGPVITPCTRDGINKIFLGGCDARVFCMGV 300

Query: 2266 RIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPVN 2087
            RIVKRRTL QVLN+IPKE EGE FEDALARVRRCVGGGAATENADSDSDIEVVADFIPVN
Sbjct: 301  RIVKRRTLQQVLNMIPKEDEGESFEDALARVRRCVGGGAATENADSDSDIEVVADFIPVN 360

Query: 2086 LRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPYF 1907
            LRCPMSGLRMKVAGRF+ C HMGCFDLEVFVEMN RSRKWQCPICLKNYSLEKIIIDPYF
Sbjct: 361  LRCPMSGLRMKVAGRFKHCAHMGCFDLEVFVEMNQRSRKWQCPICLKNYSLEKIIIDPYF 420

Query: 1906 NRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEGESKPK 1727
            NRITSK+RNCGEDV EIEVKPDGSWRAK EGDR+ LGELGLWHLPDG +CPS+E E+KPK
Sbjct: 421  NRITSKMRNCGEDVAEIEVKPDGSWRAKAEGDRKGLGELGLWHLPDGTICPSVEAETKPK 480

Query: 1726 QEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFENDDHNVI 1547
             E +K IKQE GSD +AGL+LG+K+N  GCWE NK DDI  +S ++R +E+FE++  N+I
Sbjct: 481  VE-LKPIKQEVGSDCNAGLRLGIKKNSKGCWEFNKPDDIQGISPSNRFEENFEDNGQNII 539

Query: 1546 PMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQ--NLPAPL 1373
            PMSSSATGSGRD EDASVNQ G  NLDF+  NGI+ ESIS+N+D ++ F +Q  + PA  
Sbjct: 540  PMSSSATGSGRDCEDASVNQGGGGNLDFSAINGIDYESISMNIDPTHVFGDQMTSGPAGD 599

Query: 1372 EEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGNG----I 1205
             EVI+LSDSEEE EPL+SSGAVYKN   D  G  F  +QHGI  SY E+PA GNG    +
Sbjct: 600  AEVIVLSDSEEEIEPLMSSGAVYKNTGPDT-GVPFLAAQHGITDSYYENPAVGNGGSSCL 658

Query: 1204 DLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGYTLT 1025
             L++++DDDFG++MW LPSGSQG PGFQLFGSD DV+DALV++QHGS+NC +++NGYTL 
Sbjct: 659  GLYSSNDDDFGVNMWPLPSGSQGVPGFQLFGSDLDVSDALVEMQHGSLNCSSSINGYTLA 718

Query: 1024 AETAMGSAALVPESSVKHTNVNDGLVDNPLAFSGNDPSLQIFLPTRPSDAS-AQADMRDL 848
            AETAMGSAALVPES+ +HTN ND LVDNPLAFSGND SLQIFLPTRPSDAS A +++ D 
Sbjct: 719  AETAMGSAALVPESTSQHTNTNDALVDNPLAFSGNDSSLQIFLPTRPSDASAAPSELGDH 778

Query: 847  PDVSNGIRXVEGVMVSL 797
            PDV N IR  + + + L
Sbjct: 779  PDVLNAIRTEDWISLRL 795



 Score = 98.2 bits (243), Expect = 4e-17
 Identities = 52/78 (66%), Positives = 56/78 (71%)
 Frame = -2

Query: 818  GGGHGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXGMNDNKSGKASRERSDSPFSFPR 639
            GGG  E AA+N LSSG QLQ K               G+NDN+SGK SRERSDSPF+FPR
Sbjct: 800  GGGQAESAAANDLSSGQQLQPKDSTLDSLAENASLLLGINDNRSGKTSRERSDSPFTFPR 859

Query: 638  QRRSVRPRLYLSIDSDSE 585
            QRRSVRPRLYLSIDSDSE
Sbjct: 860  QRRSVRPRLYLSIDSDSE 877


>ref|XP_011089333.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like isoform X3 [Sesamum
            indicum]
          Length = 874

 Score = 1256 bits (3249), Expect = 0.0
 Identities = 613/797 (76%), Positives = 696/797 (87%), Gaps = 7/797 (0%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLV++CKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSD+RVSG+WAKKNAV
Sbjct: 1    MDLVSNCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDERVSGLWAKKNAV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLCGSTL 2807
            GKE+VAKLVDDTYRKMQ+SGA+DLASKS + +   N++PKEETE+ +QM+KIRCLCGSTL
Sbjct: 61   GKEDVAKLVDDTYRKMQVSGASDLASKSQTVSDNSNIRPKEETEDRHQMEKIRCLCGSTL 120

Query: 2806 PTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEIFYCEICRLSRADPFWMTVAHP 2627
            PTDSMIKCEDPRCNVWQH+ACV+IPEKP EG+LPN P+IFYCE+CRLSRADPFW+TVAHP
Sbjct: 121  PTDSMIKCEDPRCNVWQHMACVLIPEKPTEGILPNPPDIFYCEVCRLSRADPFWVTVAHP 180

Query: 2626 LYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQW 2447
            LYPVKL IT+VP+DG    QSIEKTF L RAD+DLLSKQEYDVQAWCMLLNDKV FRMQW
Sbjct: 181  LYPVKLNITNVPADG----QSIEKTFSLARADRDLLSKQEYDVQAWCMLLNDKVTFRMQW 236

Query: 2446 PLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLGV 2267
            P YADLQ+NGVPVRAINRPGSQ LGANGRDDGP ITPCTRDG+NKI + GCDARIFC+GV
Sbjct: 237  PQYADLQVNGVPVRAINRPGSQLLGANGRDDGPVITPCTRDGLNKIFVGGCDARIFCMGV 296

Query: 2266 RIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPVN 2087
            RI KRR+L QVLN+IPKE EGERFEDALA VRRCVGGGAATENADSDSDIEVVADFIPVN
Sbjct: 297  RIAKRRSLQQVLNMIPKEDEGERFEDALAPVRRCVGGGAATENADSDSDIEVVADFIPVN 356

Query: 2086 LRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPYF 1907
            LRCPMSGLRMKVAGRF+ C HMGCFDLEVFVEMN RSRKWQCPICLKNYSLEKIIIDPYF
Sbjct: 357  LRCPMSGLRMKVAGRFKHCAHMGCFDLEVFVEMNQRSRKWQCPICLKNYSLEKIIIDPYF 416

Query: 1906 NRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEGESKPK 1727
            NRITSK+RNCGEDV EIEVKPDGSWRAK EGDR+ +GELGLWHLPDG++C S + +SKPK
Sbjct: 417  NRITSKMRNCGEDVAEIEVKPDGSWRAKAEGDRKGVGELGLWHLPDGSICASADEDSKPK 476

Query: 1726 QEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFENDDHNVI 1547
             E +K IKQE GSDS+AGLKLG+K+NRNGCWE +K D++  +++A+R  E+FE +  N+I
Sbjct: 477  LE-LKPIKQEAGSDSNAGLKLGIKKNRNGCWEFHKPDNMQGITTANRFDENFEKNGQNII 535

Query: 1546 PMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQNLPAPL-- 1373
            PMSSSATGSG+D ED SVNQ G  NLDF+T NGIE ESI LN+D ++GF+++   AP   
Sbjct: 536  PMSSSATGSGKDCEDGSVNQVGGGNLDFSTVNGIEYESIPLNIDPTHGFSDRTTSAPAGE 595

Query: 1372 EEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGN----GI 1205
             EVI+LSDSEEE EPL+SS A YKN   D GG  FP +QHGIP SY E PA GN     +
Sbjct: 596  AEVIVLSDSEEEIEPLMSSEAGYKNTGPDTGGVPFPAAQHGIPDSYYEHPALGNVGSSCL 655

Query: 1204 DLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGYTLT 1025
             L+N +DDDFG++MW+LPSGS G PGFQLFGSD DV++ALV++QHGS+NC +++NGY LT
Sbjct: 656  GLYNTNDDDFGVNMWTLPSGSHGGPGFQLFGSDVDVSEALVEMQHGSLNCSSSINGYALT 715

Query: 1024 AETAMGSAALVPESSVKHTNVNDGLVDNPLAFSGNDPSLQIFLPTRPSDAS-AQADMRDL 848
            AETAMGSAALV ESS + +N NDGLVDNPLAFSGNDPSLQIFLPTRPSDAS A +D+RD 
Sbjct: 716  AETAMGSAALVSESSAQRSNTNDGLVDNPLAFSGNDPSLQIFLPTRPSDASAAPSDLRDH 775

Query: 847  PDVSNGIRXVEGVMVSL 797
            PDVSNGIR  + + + L
Sbjct: 776  PDVSNGIRAKDWISLRL 792



 Score = 95.5 bits (236), Expect = 2e-16
 Identities = 50/78 (64%), Positives = 56/78 (71%)
 Frame = -2

Query: 818  GGGHGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXGMNDNKSGKASRERSDSPFSFPR 639
            G G GE AA++ ++S  QLQSK               GMNDN+SGK SRERSDSPF+FPR
Sbjct: 797  GAGQGESAAASGINSAQQLQSKDDTLNSLADNASLLLGMNDNRSGKTSRERSDSPFTFPR 856

Query: 638  QRRSVRPRLYLSIDSDSE 585
            QRRSVRPRLYLSIDSDSE
Sbjct: 857  QRRSVRPRLYLSIDSDSE 874


>emb|CDP07144.1| unnamed protein product [Coffea canephora]
          Length = 874

 Score = 1230 bits (3182), Expect = 0.0
 Identities = 603/799 (75%), Positives = 681/799 (85%), Gaps = 9/799 (1%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLV +CKDKLAYFRIKELKDVLTQLG+SKQGKKQDLVDRIL +LSDD+VSGMWAKKNAV
Sbjct: 1    MDLVANCKDKLAYFRIKELKDVLTQLGMSKQGKKQDLVDRILNVLSDDQVSGMWAKKNAV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLCGSTL 2807
            GK++VAKLVDDTYRKMQ+SGATDLASKS   +   NVK KEE E+ YQ +KIRC CGS+L
Sbjct: 61   GKDDVAKLVDDTYRKMQVSGATDLASKSQGGSECSNVKLKEEIEDSYQTEKIRCPCGSSL 120

Query: 2806 PTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEIFYCEICRLSRADPFWMTVAHP 2627
            P ++MIKCEDP+C VWQHI CV+I EKP EG LP  PE FYCE+CRL+RADPFW+T+AHP
Sbjct: 121  PNETMIKCEDPKCGVWQHIGCVIISEKPNEGALPVPPETFYCELCRLTRADPFWVTMAHP 180

Query: 2626 LYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQW 2447
            LYPVKL ITSVP+DGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQW
Sbjct: 181  LYPVKLLITSVPADGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQW 240

Query: 2446 PLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLGV 2267
            P YADLQ+NGVPVRAINRPGSQ LGANGRDDGP ITPCTRDGIN+ISLTGCDAR+FCLGV
Sbjct: 241  PQYADLQVNGVPVRAINRPGSQLLGANGRDDGPVITPCTRDGINRISLTGCDARVFCLGV 300

Query: 2266 RIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPVN 2087
            RIVKRRT+  +LN+IPKESEGE FEDALARV+RCVGGG  TENADSDSD+EVVADFIPVN
Sbjct: 301  RIVKRRTVQHILNLIPKESEGELFEDALARVKRCVGGGTETENADSDSDLEVVADFIPVN 360

Query: 2086 LRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPYF 1907
            LRCPMSG RMKVAGRF+PCVHMGCFDLEVFVEMN+RSRKWQCPICLKNY LE IIIDPYF
Sbjct: 361  LRCPMSGSRMKVAGRFKPCVHMGCFDLEVFVEMNMRSRKWQCPICLKNYCLENIIIDPYF 420

Query: 1906 NRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEGESKPK 1727
            NRITSK+R+ GE+VNEIEVKPDGSWRAK EGDRR LG+LGLWH PDG+L  S + ESKPK
Sbjct: 421  NRITSKLRSYGEEVNEIEVKPDGSWRAKVEGDRRGLGDLGLWHAPDGSL--SADVESKPK 478

Query: 1726 QEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFENDDHNVI 1547
               +KQIKQEGGSD HAGLKLGMK+NRNG WE++K +D+   SS + L E F +   N+I
Sbjct: 479  PVMLKQIKQEGGSDGHAGLKLGMKKNRNGIWEISKPEDLQTFSSGNNLNEDFCH-RQNII 537

Query: 1546 PMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQNLPAPL-- 1373
            PMSSSAT + +DGED SVNQDG  NLD++TNNGIELESISLN++ +YG++++N  APL  
Sbjct: 538  PMSSSATSTSKDGEDPSVNQDGGGNLDYSTNNGIELESISLNIEPTYGYSDRNPIAPLGD 597

Query: 1372 EEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGNGI---- 1205
             EVI+LSDSEEEN+PLISSG +YKN+H D G  ++ ++  GI  SYPEDP    G     
Sbjct: 598  TEVIVLSDSEEENQPLISSGHIYKNHHTDGGEVSYSVAAQGILDSYPEDPTLNVGAGSCP 657

Query: 1204 DLFNAHDDDFGMHMWS-LPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGYTL 1028
               N HDDD    MWS LPSG Q  PGFQLFGSD DV+DALVD+Q GSINC +++NGYTL
Sbjct: 658  SFLNGHDDDL---MWSLLPSGGQAGPGFQLFGSDGDVSDALVDMQQGSINCTSSINGYTL 714

Query: 1027 TAETAMGSAALVPESSVK--HTNVNDGLVDNPLAFSGNDPSLQIFLPTRPSDASAQADMR 854
             AETAMGSAALVPESS++  H N+NDGLVDNPL F GNDPSLQIFLPTRP+DAS QA++R
Sbjct: 715  GAETAMGSAALVPESSIEPVHGNINDGLVDNPLVFGGNDPSLQIFLPTRPTDASVQAELR 774

Query: 853  DLPDVSNGIRXVEGVMVSL 797
            D PDVSNGI   + + + L
Sbjct: 775  DQPDVSNGIHTEDWISLRL 793



 Score = 86.7 bits (213), Expect = 1e-13
 Identities = 49/79 (62%), Positives = 54/79 (68%)
 Frame = -2

Query: 821  GGGGHGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXGMNDNKSGKASRERSDSPFSFP 642
            GGG  GE AA+N L+SG  LQSK                M+D +S KASRERSDSPF+FP
Sbjct: 797  GGGSTGESAAANGLNSGPPLQSKEGALDSLAEASLLLG-MSDGRSSKASRERSDSPFTFP 855

Query: 641  RQRRSVRPRLYLSIDSDSE 585
            RQRRSVRPRL LSIDSDSE
Sbjct: 856  RQRRSVRPRLLLSIDSDSE 874


>ref|XP_011089337.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isoform X2 [Sesamum indicum]
          Length = 841

 Score = 1182 bits (3058), Expect = 0.0
 Identities = 593/797 (74%), Positives = 659/797 (82%), Gaps = 7/797 (0%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLV SCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSD+RVSG+WAKKNAV
Sbjct: 1    MDLVASCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDERVSGLWAKKNAV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLCGSTL 2807
            GKE+VAKLVDDTYRKMQ+SGA DLASKS   +   N+K KEE E+ YQM+KIRC+CGSTL
Sbjct: 61   GKEDVAKLVDDTYRKMQVSGAADLASKSQVVSDSTNIKCKEEAEDCYQMEKIRCVCGSTL 120

Query: 2806 PTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEIFYCEICRLSRADPFWMTVAHP 2627
            PTDSMIKCEDPRCNVWQH+ACV+IPEKP EGVLPN P+IFYCE+CRLSRADPFW+TVAHP
Sbjct: 121  PTDSMIKCEDPRCNVWQHMACVLIPEKPTEGVLPNPPDIFYCEVCRLSRADPFWVTVAHP 180

Query: 2626 LYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQW 2447
            LYPVKL IT+VP+DGSNP QSIEKTF LTRAD+DLLSKQEYDVQAWCMLLNDKV FRMQW
Sbjct: 181  LYPVKLNITNVPADGSNPSQSIEKTFHLTRADRDLLSKQEYDVQAWCMLLNDKVTFRMQW 240

Query: 2446 PLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLGV 2267
            P YADLQ+NGVPVRAINRPGSQ LGANGRDDGP ITPCTRDGINKI L GCDAR+FC+GV
Sbjct: 241  PQYADLQVNGVPVRAINRPGSQLLGANGRDDGPVITPCTRDGINKIFLGGCDARVFCMGV 300

Query: 2266 RIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPVN 2087
            RIVKRRTL QVLN+IPKE EGE FEDALARVRRCVGGGAATENADSDSDIEVVADFIPVN
Sbjct: 301  RIVKRRTLQQVLNMIPKEDEGESFEDALARVRRCVGGGAATENADSDSDIEVVADFIPVN 360

Query: 2086 LRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPYF 1907
            LRCPMSGLRMKVAGRF+ C HMGCFDLEVFVEMN RSRKWQCPICLKNYSLEKIIIDPYF
Sbjct: 361  LRCPMSGLRMKVAGRFKHCAHMGCFDLEVFVEMNQRSRKWQCPICLKNYSLEKIIIDPYF 420

Query: 1906 NRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEGESKPK 1727
            NRITSK+RNCGEDV EIEVKPDGSWRAK EGDR+ LGELGLWHLPDG +CPS+E E+KPK
Sbjct: 421  NRITSKMRNCGEDVAEIEVKPDGSWRAKAEGDRKGLGELGLWHLPDGTICPSVEAETKPK 480

Query: 1726 QEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFENDDHNVI 1547
             E +K IKQE GSD +AGL+LG+K+N  GCWE NK DDI  +S ++R +E+FE++  N+I
Sbjct: 481  VE-LKPIKQEVGSDCNAGLRLGIKKNSKGCWEFNKPDDIQGISPSNRFEENFEDNGQNII 539

Query: 1546 PMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQNLPAPL-- 1373
            PMSSSATGSGRD EDASVNQ G  NLDF+  NGI+ ESIS+N+D ++ F +Q    P   
Sbjct: 540  PMSSSATGSGRDCEDASVNQGGGGNLDFSAINGIDYESISMNIDPTHVFGDQMTSGPAGD 599

Query: 1372 EEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGNG----I 1205
             EVI+LSDSEEE EPL+SSGAVYKN   D G   F  +QHGI  SY E+PA GNG    +
Sbjct: 600  AEVIVLSDSEEEIEPLMSSGAVYKNTGPDTG-VPFLAAQHGITDSYYENPAVGNGGSSCL 658

Query: 1204 DLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGYTLT 1025
             L++++DDDFG++MW LPSGSQG P                                   
Sbjct: 659  GLYSSNDDDFGVNMWPLPSGSQGVP----------------------------------- 683

Query: 1024 AETAMGSAALVPESSVKHTNVNDGLVDNPLAFSGNDPSLQIFLPTRPSDAS-AQADMRDL 848
             ETAMGSAALVPES+ +HTN ND LVDNPLAFSGND SLQIFLPTRPSDAS A +++ D 
Sbjct: 684  -ETAMGSAALVPESTSQHTNTNDALVDNPLAFSGNDSSLQIFLPTRPSDASAAPSELGDH 742

Query: 847  PDVSNGIRXVEGVMVSL 797
            PDV N IR  + + + L
Sbjct: 743  PDVLNAIRTEDWISLRL 759



 Score = 98.2 bits (243), Expect = 4e-17
 Identities = 52/78 (66%), Positives = 56/78 (71%)
 Frame = -2

Query: 818 GGGHGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXGMNDNKSGKASRERSDSPFSFPR 639
           GGG  E AA+N LSSG QLQ K               G+NDN+SGK SRERSDSPF+FPR
Sbjct: 764 GGGQAESAAANDLSSGQQLQPKDSTLDSLAENASLLLGINDNRSGKTSRERSDSPFTFPR 823

Query: 638 QRRSVRPRLYLSIDSDSE 585
           QRRSVRPRLYLSIDSDSE
Sbjct: 824 QRRSVRPRLYLSIDSDSE 841


>ref|XP_011089338.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isoform X3 [Sesamum indicum]
          Length = 835

 Score = 1169 bits (3024), Expect = 0.0
 Identities = 589/797 (73%), Positives = 656/797 (82%), Gaps = 7/797 (0%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLV SCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSD+RVSG+WAKKNAV
Sbjct: 1    MDLVASCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDERVSGLWAKKNAV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLCGSTL 2807
            GKE+VAKLVDDTYRKMQ+SGA DLASKS   +   N+K KEE E+ YQM+KIRC+CGSTL
Sbjct: 61   GKEDVAKLVDDTYRKMQVSGAADLASKSQVVSDSTNIKCKEEAEDCYQMEKIRCVCGSTL 120

Query: 2806 PTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEIFYCEICRLSRADPFWMTVAHP 2627
            PTDSMIKCEDPRCNVWQH+ACV+IPEKP EGVLPN P+IFYCE+CRLSRADPFW+TVAHP
Sbjct: 121  PTDSMIKCEDPRCNVWQHMACVLIPEKPTEGVLPNPPDIFYCEVCRLSRADPFWVTVAHP 180

Query: 2626 LYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQW 2447
            LYPVKL IT+VP+DGSNP QSIEKTF LTRAD+DLLSKQEYDVQAWCMLLNDKV FRMQW
Sbjct: 181  LYPVKLNITNVPADGSNPSQSIEKTFHLTRADRDLLSKQEYDVQAWCMLLNDKVTFRMQW 240

Query: 2446 PLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLGV 2267
            P YADLQ+NGVPVRAINRPGSQ LGANGRDDGP ITPCTRDGINKI L GCDAR+FC+GV
Sbjct: 241  PQYADLQVNGVPVRAINRPGSQLLGANGRDDGPVITPCTRDGINKIFLGGCDARVFCMGV 300

Query: 2266 RIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPVN 2087
            RIVKRRTL QVLN+IPKE EGE FEDALARVRRCVGGGAATENADSDSDIEVVADFIPVN
Sbjct: 301  RIVKRRTLQQVLNMIPKEDEGESFEDALARVRRCVGGGAATENADSDSDIEVVADFIPVN 360

Query: 2086 LRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPYF 1907
            LRCPMSGLRMKVAGRF+ C HMGCFDLEVFVEMN RSRKWQCPICLKNYSLEKIIIDPYF
Sbjct: 361  LRCPMSGLRMKVAGRFKHCAHMGCFDLEVFVEMNQRSRKWQCPICLKNYSLEKIIIDPYF 420

Query: 1906 NRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEGESKPK 1727
            NRITSK+RNCGEDV EIEVKPDGSWRAK EGDR+ LGELGLWHLPDG +CPS+E E+KPK
Sbjct: 421  NRITSKMRNCGEDVAEIEVKPDGSWRAKAEGDRKGLGELGLWHLPDGTICPSVEAETKPK 480

Query: 1726 QEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFENDDHNVI 1547
             E +K IKQE GSD +AGL+LG+K+N  GCWE NK DDI  +S ++R +E+FE++  N+I
Sbjct: 481  VE-LKPIKQEVGSDCNAGLRLGIKKNSKGCWEFNKPDDIQGISPSNRFEENFEDNGQNII 539

Query: 1546 PMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQ--NLPAPL 1373
            PMSSSATGSGRD EDASVNQ G  NLDF+  NGI+ ESIS+N+D ++ F +Q  + PA  
Sbjct: 540  PMSSSATGSGRDCEDASVNQGGGGNLDFSAINGIDYESISMNIDPTHVFGDQMTSGPAGD 599

Query: 1372 EEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGNG----I 1205
             EVI+LSDSEEE EPL+SSGAVYKN   D  G  F  +QHGI  SY E+PA GNG    +
Sbjct: 600  AEVIVLSDSEEEIEPLMSSGAVYKNTGPDT-GVPFLAAQHGITDSYYENPAVGNGGSSCL 658

Query: 1204 DLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGYTLT 1025
             L++++DDDFG++MW LPS                                         
Sbjct: 659  GLYSSNDDDFGVNMWPLPS----------------------------------------- 677

Query: 1024 AETAMGSAALVPESSVKHTNVNDGLVDNPLAFSGNDPSLQIFLPTRPSDAS-AQADMRDL 848
             ETAMGSAALVPES+ +HTN ND LVDNPLAFSGND SLQIFLPTRPSDAS A +++ D 
Sbjct: 678  -ETAMGSAALVPESTSQHTNTNDALVDNPLAFSGNDSSLQIFLPTRPSDASAAPSELGDH 736

Query: 847  PDVSNGIRXVEGVMVSL 797
            PDV N IR  + + + L
Sbjct: 737  PDVLNAIRTEDWISLRL 753



 Score = 98.2 bits (243), Expect = 4e-17
 Identities = 52/78 (66%), Positives = 56/78 (71%)
 Frame = -2

Query: 818 GGGHGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXGMNDNKSGKASRERSDSPFSFPR 639
           GGG  E AA+N LSSG QLQ K               G+NDN+SGK SRERSDSPF+FPR
Sbjct: 758 GGGQAESAAANDLSSGQQLQPKDSTLDSLAENASLLLGINDNRSGKTSRERSDSPFTFPR 817

Query: 638 QRRSVRPRLYLSIDSDSE 585
           QRRSVRPRLYLSIDSDSE
Sbjct: 818 QRRSVRPRLYLSIDSDSE 835


>ref|XP_009787730.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isoform X3 [Nicotiana
            sylvestris]
          Length = 833

 Score = 1144 bits (2959), Expect = 0.0
 Identities = 565/798 (70%), Positives = 666/798 (83%), Gaps = 8/798 (1%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLV SCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILA LSD+RVSGMW KKN+V
Sbjct: 1    MDLVASCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILATLSDERVSGMWPKKNSV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLCGSTL 2807
            GKE+VAKLVDD YRKMQ+ GAT+LASKS   +   NVK KEE E+     KIRC+C S+L
Sbjct: 61   GKEDVAKLVDDIYRKMQVGGATELASKSQVVSDSSNVKLKEEIEDSSYHMKIRCVCASSL 120

Query: 2806 PTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEI-FYCEICRLSRADPFWMTVAH 2630
             T++MI+CED RC+ WQH+ CV+IP+KP+EG  P  P   FYCE+CRL RADPFW+T+ H
Sbjct: 121  QTETMIQCEDRRCHTWQHVRCVIIPDKPMEGGDPPIPPTTFYCELCRLGRADPFWVTMGH 180

Query: 2629 PLYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQ 2450
            PLYP KL+ITSVP+DG+NPVQSIEKTFQ+TRAD+DLL+KQEYD+QAWCMLLNDKV FRMQ
Sbjct: 181  PLYPAKLSITSVPADGTNPVQSIEKTFQITRADRDLLAKQEYDLQAWCMLLNDKVQFRMQ 240

Query: 2449 WPLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLG 2270
            WP YADLQ+NGVPVRAINRPGSQ LGANGRDDGP ITPCTRDGINKI+LTGCDAR+FCLG
Sbjct: 241  WPQYADLQVNGVPVRAINRPGSQLLGANGRDDGPIITPCTRDGINKITLTGCDARVFCLG 300

Query: 2269 VRIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPV 2090
            VR+VKRRT+ QVL++IPKES+GE+FEDAL RVRRCVGGG ATENADSDSD+EVVAD IPV
Sbjct: 301  VRLVKRRTVQQVLSLIPKESDGEQFEDALTRVRRCVGGGTATENADSDSDLEVVADCIPV 360

Query: 2089 NLRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPY 1910
            NLRCPMSG RMKVAGRF+PC+HMGCFDLEVFVEMN RSRKWQCPICLKNY+LE +IIDPY
Sbjct: 361  NLRCPMSGSRMKVAGRFKPCIHMGCFDLEVFVEMNQRSRKWQCPICLKNYALEHVIIDPY 420

Query: 1909 FNRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEGESKP 1730
            FNRITS++R+CGEDV EIEVKPDGSWRAK EGDRR+LG+LG WHLPDG+L  S++ ESKP
Sbjct: 421  FNRITSQLRSCGEDVAEIEVKPDGSWRAKIEGDRRSLGDLGCWHLPDGSLSESLDIESKP 480

Query: 1729 KQEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFENDDHNV 1550
            K E +KQIKQEGGSD + GLK+G+++N NG WE++K +D    SS SRL+++F  D   +
Sbjct: 481  KPEILKQIKQEGGSDGN-GLKVGLRKNINGLWEISKPED-QTFSSGSRLRDNFGQD---I 535

Query: 1549 IPMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQN--LPAP 1376
            IPMSS ATGSG++GEDASVNQDG  NLDF +NN  +LE+ISL +D +YGF   N  +PA 
Sbjct: 536  IPMSSGATGSGKEGEDASVNQDGNGNLDF-SNNAFDLEAISL-IDPTYGFGNGNPSVPAG 593

Query: 1375 LEEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGNG---I 1205
              EVI+LSDSEEENEP+ISSG ++ NNH D     FP    GIP ++ +     +G   +
Sbjct: 594  DAEVIVLSDSEEENEPIISSGPIFNNNHNDAPVVPFPGQSQGIPDAFHDSTLVNSGNSCL 653

Query: 1204 DLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGYTLT 1025
             LFN++DD+FGM+MWSLPSG+QG PGFQLF SDADV+ +LVDVQHGSINCP+++ GY L 
Sbjct: 654  GLFNSNDDEFGMNMWSLPSGTQGGPGFQLFSSDADVSGSLVDVQHGSINCPSSLGGYGLA 713

Query: 1024 AETAMGSAALVPESSVK--HTNVNDGLVDNPLAFSGNDPSLQIFLPTRPSDASAQADMRD 851
            A+T +GS +L+PE+     + N+ND LVDNPLAFSGNDPSLQIFLPTRPSD S +A  RD
Sbjct: 714  ADTGIGSTSLLPETYANRPNANINDSLVDNPLAFSGNDPSLQIFLPTRPSDTSVEA-TRD 772

Query: 850  LPDVSNGIRXVEGVMVSL 797
             PDVSNG+   + + + L
Sbjct: 773  QPDVSNGVGTEDWISLRL 790


>ref|XP_009787729.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isoform X2 [Nicotiana
            sylvestris]
          Length = 870

 Score = 1144 bits (2959), Expect = 0.0
 Identities = 565/798 (70%), Positives = 666/798 (83%), Gaps = 8/798 (1%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLV SCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILA LSD+RVSGMW KKN+V
Sbjct: 1    MDLVASCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILATLSDERVSGMWPKKNSV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLCGSTL 2807
            GKE+VAKLVDD YRKMQ+ GAT+LASKS   +   NVK KEE E+     KIRC+C S+L
Sbjct: 61   GKEDVAKLVDDIYRKMQVGGATELASKSQVVSDSSNVKLKEEIEDSSYHMKIRCVCASSL 120

Query: 2806 PTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEI-FYCEICRLSRADPFWMTVAH 2630
             T++MI+CED RC+ WQH+ CV+IP+KP+EG  P  P   FYCE+CRL RADPFW+T+ H
Sbjct: 121  QTETMIQCEDRRCHTWQHVRCVIIPDKPMEGGDPPIPPTTFYCELCRLGRADPFWVTMGH 180

Query: 2629 PLYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQ 2450
            PLYP KL+ITSVP+DG+NPVQSIEKTFQ+TRAD+DLL+KQEYD+QAWCMLLNDKV FRMQ
Sbjct: 181  PLYPAKLSITSVPADGTNPVQSIEKTFQITRADRDLLAKQEYDLQAWCMLLNDKVQFRMQ 240

Query: 2449 WPLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLG 2270
            WP YADLQ+NGVPVRAINRPGSQ LGANGRDDGP ITPCTRDGINKI+LTGCDAR+FCLG
Sbjct: 241  WPQYADLQVNGVPVRAINRPGSQLLGANGRDDGPIITPCTRDGINKITLTGCDARVFCLG 300

Query: 2269 VRIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPV 2090
            VR+VKRRT+ QVL++IPKES+GE+FEDAL RVRRCVGGG ATENADSDSD+EVVAD IPV
Sbjct: 301  VRLVKRRTVQQVLSLIPKESDGEQFEDALTRVRRCVGGGTATENADSDSDLEVVADCIPV 360

Query: 2089 NLRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPY 1910
            NLRCPMSG RMKVAGRF+PC+HMGCFDLEVFVEMN RSRKWQCPICLKNY+LE +IIDPY
Sbjct: 361  NLRCPMSGSRMKVAGRFKPCIHMGCFDLEVFVEMNQRSRKWQCPICLKNYALEHVIIDPY 420

Query: 1909 FNRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEGESKP 1730
            FNRITS++R+CGEDV EIEVKPDGSWRAK EGDRR+LG+LG WHLPDG+L  S++ ESKP
Sbjct: 421  FNRITSQLRSCGEDVAEIEVKPDGSWRAKIEGDRRSLGDLGCWHLPDGSLSESLDIESKP 480

Query: 1729 KQEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFENDDHNV 1550
            K E +KQIKQEGGSD + GLK+G+++N NG WE++K +D    SS SRL+++F  D   +
Sbjct: 481  KPEILKQIKQEGGSDGN-GLKVGLRKNINGLWEISKPED-QTFSSGSRLRDNFGQD---I 535

Query: 1549 IPMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQN--LPAP 1376
            IPMSS ATGSG++GEDASVNQDG  NLDF +NN  +LE+ISL +D +YGF   N  +PA 
Sbjct: 536  IPMSSGATGSGKEGEDASVNQDGNGNLDF-SNNAFDLEAISL-IDPTYGFGNGNPSVPAG 593

Query: 1375 LEEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGNG---I 1205
              EVI+LSDSEEENEP+ISSG ++ NNH D     FP    GIP ++ +     +G   +
Sbjct: 594  DAEVIVLSDSEEENEPIISSGPIFNNNHNDAPVVPFPGQSQGIPDAFHDSTLVNSGNSCL 653

Query: 1204 DLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGYTLT 1025
             LFN++DD+FGM+MWSLPSG+QG PGFQLF SDADV+ +LVDVQHGSINCP+++ GY L 
Sbjct: 654  GLFNSNDDEFGMNMWSLPSGTQGGPGFQLFSSDADVSGSLVDVQHGSINCPSSLGGYGLA 713

Query: 1024 AETAMGSAALVPESSVK--HTNVNDGLVDNPLAFSGNDPSLQIFLPTRPSDASAQADMRD 851
            A+T +GS +L+PE+     + N+ND LVDNPLAFSGNDPSLQIFLPTRPSD S +A  RD
Sbjct: 714  ADTGIGSTSLLPETYANRPNANINDSLVDNPLAFSGNDPSLQIFLPTRPSDTSVEA-TRD 772

Query: 850  LPDVSNGIRXVEGVMVSL 797
             PDVSNG+   + + + L
Sbjct: 773  QPDVSNGVGTEDWISLRL 790



 Score = 87.0 bits (214), Expect = 9e-14
 Identities = 48/78 (61%), Positives = 55/78 (70%)
 Frame = -2

Query: 818  GGGHGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXGMNDNKSGKASRERSDSPFSFPR 639
            GG HG+  A N LSSG Q+Q+K               GMND++  K+SRERSDSPF+FPR
Sbjct: 794  GGVHGDSVA-NGLSSGQQVQTKDTALDSLADTASLLLGMNDSRPTKSSRERSDSPFTFPR 852

Query: 638  QRRSVRPRLYLSIDSDSE 585
            QRRSVRPRLYLSIDSDSE
Sbjct: 853  QRRSVRPRLYLSIDSDSE 870


>ref|XP_009787726.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isoform X1 [Nicotiana
            sylvestris] gi|698481473|ref|XP_009787727.1| PREDICTED:
            E3 SUMO-protein ligase SIZ1 isoform X1 [Nicotiana
            sylvestris] gi|698481476|ref|XP_009787728.1| PREDICTED:
            E3 SUMO-protein ligase SIZ1 isoform X1 [Nicotiana
            sylvestris]
          Length = 878

 Score = 1144 bits (2959), Expect = 0.0
 Identities = 565/798 (70%), Positives = 666/798 (83%), Gaps = 8/798 (1%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLV SCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILA LSD+RVSGMW KKN+V
Sbjct: 1    MDLVASCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILATLSDERVSGMWPKKNSV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLCGSTL 2807
            GKE+VAKLVDD YRKMQ+ GAT+LASKS   +   NVK KEE E+     KIRC+C S+L
Sbjct: 61   GKEDVAKLVDDIYRKMQVGGATELASKSQVVSDSSNVKLKEEIEDSSYHMKIRCVCASSL 120

Query: 2806 PTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEI-FYCEICRLSRADPFWMTVAH 2630
             T++MI+CED RC+ WQH+ CV+IP+KP+EG  P  P   FYCE+CRL RADPFW+T+ H
Sbjct: 121  QTETMIQCEDRRCHTWQHVRCVIIPDKPMEGGDPPIPPTTFYCELCRLGRADPFWVTMGH 180

Query: 2629 PLYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQ 2450
            PLYP KL+ITSVP+DG+NPVQSIEKTFQ+TRAD+DLL+KQEYD+QAWCMLLNDKV FRMQ
Sbjct: 181  PLYPAKLSITSVPADGTNPVQSIEKTFQITRADRDLLAKQEYDLQAWCMLLNDKVQFRMQ 240

Query: 2449 WPLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLG 2270
            WP YADLQ+NGVPVRAINRPGSQ LGANGRDDGP ITPCTRDGINKI+LTGCDAR+FCLG
Sbjct: 241  WPQYADLQVNGVPVRAINRPGSQLLGANGRDDGPIITPCTRDGINKITLTGCDARVFCLG 300

Query: 2269 VRIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPV 2090
            VR+VKRRT+ QVL++IPKES+GE+FEDAL RVRRCVGGG ATENADSDSD+EVVAD IPV
Sbjct: 301  VRLVKRRTVQQVLSLIPKESDGEQFEDALTRVRRCVGGGTATENADSDSDLEVVADCIPV 360

Query: 2089 NLRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPY 1910
            NLRCPMSG RMKVAGRF+PC+HMGCFDLEVFVEMN RSRKWQCPICLKNY+LE +IIDPY
Sbjct: 361  NLRCPMSGSRMKVAGRFKPCIHMGCFDLEVFVEMNQRSRKWQCPICLKNYALEHVIIDPY 420

Query: 1909 FNRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEGESKP 1730
            FNRITS++R+CGEDV EIEVKPDGSWRAK EGDRR+LG+LG WHLPDG+L  S++ ESKP
Sbjct: 421  FNRITSQLRSCGEDVAEIEVKPDGSWRAKIEGDRRSLGDLGCWHLPDGSLSESLDIESKP 480

Query: 1729 KQEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFENDDHNV 1550
            K E +KQIKQEGGSD + GLK+G+++N NG WE++K +D    SS SRL+++F  D   +
Sbjct: 481  KPEILKQIKQEGGSDGN-GLKVGLRKNINGLWEISKPED-QTFSSGSRLRDNFGQD---I 535

Query: 1549 IPMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQN--LPAP 1376
            IPMSS ATGSG++GEDASVNQDG  NLDF +NN  +LE+ISL +D +YGF   N  +PA 
Sbjct: 536  IPMSSGATGSGKEGEDASVNQDGNGNLDF-SNNAFDLEAISL-IDPTYGFGNGNPSVPAG 593

Query: 1375 LEEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGNG---I 1205
              EVI+LSDSEEENEP+ISSG ++ NNH D     FP    GIP ++ +     +G   +
Sbjct: 594  DAEVIVLSDSEEENEPIISSGPIFNNNHNDAPVVPFPGQSQGIPDAFHDSTLVNSGNSCL 653

Query: 1204 DLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGYTLT 1025
             LFN++DD+FGM+MWSLPSG+QG PGFQLF SDADV+ +LVDVQHGSINCP+++ GY L 
Sbjct: 654  GLFNSNDDEFGMNMWSLPSGTQGGPGFQLFSSDADVSGSLVDVQHGSINCPSSLGGYGLA 713

Query: 1024 AETAMGSAALVPESSVK--HTNVNDGLVDNPLAFSGNDPSLQIFLPTRPSDASAQADMRD 851
            A+T +GS +L+PE+     + N+ND LVDNPLAFSGNDPSLQIFLPTRPSD S +A  RD
Sbjct: 714  ADTGIGSTSLLPETYANRPNANINDSLVDNPLAFSGNDPSLQIFLPTRPSDTSVEA-TRD 772

Query: 850  LPDVSNGIRXVEGVMVSL 797
             PDVSNG+   + + + L
Sbjct: 773  QPDVSNGVGTEDWISLRL 790



 Score = 79.7 bits (195), Expect = 1e-11
 Identities = 47/86 (54%), Positives = 54/86 (62%), Gaps = 8/86 (9%)
 Frame = -2

Query: 818  GGGHGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXG--------MNDNKSGKASRERS 663
            GG HG+  A N LSSG Q+Q+K                        MND++  K+SRERS
Sbjct: 794  GGVHGDSVA-NGLSSGQQVQTKDTALDSLADTGSDATVCAASLLLGMNDSRPTKSSRERS 852

Query: 662  DSPFSFPRQRRSVRPRLYLSIDSDSE 585
            DSPF+FPRQRRSVRPRLYLSIDSDSE
Sbjct: 853  DSPFTFPRQRRSVRPRLYLSIDSDSE 878


>ref|XP_009617749.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isoform X3 [Nicotiana
            tomentosiformis]
          Length = 833

 Score = 1142 bits (2953), Expect = 0.0
 Identities = 563/798 (70%), Positives = 665/798 (83%), Gaps = 8/798 (1%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLV SCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILA LSD+RVSGMW KKN+V
Sbjct: 1    MDLVASCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILATLSDERVSGMWPKKNSV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLCGSTL 2807
            GKE+VAKLVDD YRKMQ+ GAT+LASKS   +   NVK KEE E+     KIRC+C S+L
Sbjct: 61   GKEDVAKLVDDIYRKMQVGGATELASKSQVVSDSSNVKLKEEIEDSSYHMKIRCVCASSL 120

Query: 2806 PTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEI-FYCEICRLSRADPFWMTVAH 2630
             T++MI+CED RC+ WQH+ CV+IP+KP+EG  P  P   FYCE+CRL RADPFW+T+ H
Sbjct: 121  QTETMIQCEDRRCHTWQHVRCVIIPDKPMEGGDPPIPPTTFYCELCRLGRADPFWVTMGH 180

Query: 2629 PLYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQ 2450
            PLYP KL+ITSVP+DG+NPVQSIEKTFQ+TRAD+DLL+KQEYD+QAWCMLLNDKV FRMQ
Sbjct: 181  PLYPAKLSITSVPADGTNPVQSIEKTFQITRADRDLLAKQEYDLQAWCMLLNDKVQFRMQ 240

Query: 2449 WPLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLG 2270
            WP YADLQ+NGVPVRAINRPGSQ LGANGRDDGP ITPCTRDGINKI+LTGCDAR+FCLG
Sbjct: 241  WPQYADLQVNGVPVRAINRPGSQLLGANGRDDGPIITPCTRDGINKITLTGCDARVFCLG 300

Query: 2269 VRIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPV 2090
            VR+VKRRT+ QVL++IPKES+GE+FEDAL RVRRCVGGG ATENADSDSD+EVVAD IPV
Sbjct: 301  VRLVKRRTVQQVLSLIPKESDGEQFEDALTRVRRCVGGGTATENADSDSDLEVVADCIPV 360

Query: 2089 NLRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPY 1910
            NLRCPMSG RMKVAGRF+PC+HMGCFDLEVFVEMN RSRKWQCPICLKNY+LE +IIDPY
Sbjct: 361  NLRCPMSGSRMKVAGRFKPCIHMGCFDLEVFVEMNQRSRKWQCPICLKNYALEHVIIDPY 420

Query: 1909 FNRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEGESKP 1730
            FNRITS++R+CGEDV EIEVKPDGSWRAK EGDR++LG+LG WHLPDG+L  S++ ESKP
Sbjct: 421  FNRITSRLRSCGEDVAEIEVKPDGSWRAKIEGDRQSLGDLGRWHLPDGSLSESLDIESKP 480

Query: 1729 KQEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFENDDHNV 1550
            K E +KQIKQEGGSD + GLK+G+++NRNG WE++K +D    SS SRL+++F  D   +
Sbjct: 481  KPEILKQIKQEGGSDGN-GLKVGLRKNRNGLWEISKPED-QTFSSGSRLRDNFGQD---I 535

Query: 1549 IPMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQN--LPAP 1376
            IPMSS ATGSG++GEDASVNQDG  NLDF +NN  +LE+ISL +D +YGF  +N  +PA 
Sbjct: 536  IPMSSGATGSGKEGEDASVNQDGNGNLDF-SNNAFDLEAISL-IDPTYGFGNENPSVPAG 593

Query: 1375 LEEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGNG---I 1205
              EVI+LSDSEEENEP+ISSG ++ NNH D     FP    GIP S+ +      G   +
Sbjct: 594  DAEVIVLSDSEEENEPIISSGPIFNNNHSDAPVVPFPCQSQGIPDSFHDSTLVNGGNSCL 653

Query: 1204 DLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGYTLT 1025
             LFN++DD+FGM+MWSLPSG+QG PGFQLF SDADV+ +LVD QHGSINCP++++GY L 
Sbjct: 654  GLFNSNDDEFGMNMWSLPSGTQGGPGFQLFSSDADVSGSLVDEQHGSINCPSSLSGYGLA 713

Query: 1024 AETAMGSAALVPESSVK--HTNVNDGLVDNPLAFSGNDPSLQIFLPTRPSDASAQADMRD 851
            A+T +GS +L+P +     + N+ND LVDN LAFSGNDPSLQIFLPTRPSD S +A  RD
Sbjct: 714  ADTGIGSTSLLPGTYANRPNANINDSLVDNSLAFSGNDPSLQIFLPTRPSDTSVEA-TRD 772

Query: 850  LPDVSNGIRXVEGVMVSL 797
             PDVSNG+   + + + L
Sbjct: 773  QPDVSNGVGTEDWISLRL 790


>ref|XP_009617748.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isoform X2 [Nicotiana
            tomentosiformis]
          Length = 870

 Score = 1142 bits (2953), Expect = 0.0
 Identities = 563/798 (70%), Positives = 665/798 (83%), Gaps = 8/798 (1%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLV SCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILA LSD+RVSGMW KKN+V
Sbjct: 1    MDLVASCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILATLSDERVSGMWPKKNSV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLCGSTL 2807
            GKE+VAKLVDD YRKMQ+ GAT+LASKS   +   NVK KEE E+     KIRC+C S+L
Sbjct: 61   GKEDVAKLVDDIYRKMQVGGATELASKSQVVSDSSNVKLKEEIEDSSYHMKIRCVCASSL 120

Query: 2806 PTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEI-FYCEICRLSRADPFWMTVAH 2630
             T++MI+CED RC+ WQH+ CV+IP+KP+EG  P  P   FYCE+CRL RADPFW+T+ H
Sbjct: 121  QTETMIQCEDRRCHTWQHVRCVIIPDKPMEGGDPPIPPTTFYCELCRLGRADPFWVTMGH 180

Query: 2629 PLYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQ 2450
            PLYP KL+ITSVP+DG+NPVQSIEKTFQ+TRAD+DLL+KQEYD+QAWCMLLNDKV FRMQ
Sbjct: 181  PLYPAKLSITSVPADGTNPVQSIEKTFQITRADRDLLAKQEYDLQAWCMLLNDKVQFRMQ 240

Query: 2449 WPLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLG 2270
            WP YADLQ+NGVPVRAINRPGSQ LGANGRDDGP ITPCTRDGINKI+LTGCDAR+FCLG
Sbjct: 241  WPQYADLQVNGVPVRAINRPGSQLLGANGRDDGPIITPCTRDGINKITLTGCDARVFCLG 300

Query: 2269 VRIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPV 2090
            VR+VKRRT+ QVL++IPKES+GE+FEDAL RVRRCVGGG ATENADSDSD+EVVAD IPV
Sbjct: 301  VRLVKRRTVQQVLSLIPKESDGEQFEDALTRVRRCVGGGTATENADSDSDLEVVADCIPV 360

Query: 2089 NLRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPY 1910
            NLRCPMSG RMKVAGRF+PC+HMGCFDLEVFVEMN RSRKWQCPICLKNY+LE +IIDPY
Sbjct: 361  NLRCPMSGSRMKVAGRFKPCIHMGCFDLEVFVEMNQRSRKWQCPICLKNYALEHVIIDPY 420

Query: 1909 FNRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEGESKP 1730
            FNRITS++R+CGEDV EIEVKPDGSWRAK EGDR++LG+LG WHLPDG+L  S++ ESKP
Sbjct: 421  FNRITSRLRSCGEDVAEIEVKPDGSWRAKIEGDRQSLGDLGRWHLPDGSLSESLDIESKP 480

Query: 1729 KQEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFENDDHNV 1550
            K E +KQIKQEGGSD + GLK+G+++NRNG WE++K +D    SS SRL+++F  D   +
Sbjct: 481  KPEILKQIKQEGGSDGN-GLKVGLRKNRNGLWEISKPED-QTFSSGSRLRDNFGQD---I 535

Query: 1549 IPMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQN--LPAP 1376
            IPMSS ATGSG++GEDASVNQDG  NLDF +NN  +LE+ISL +D +YGF  +N  +PA 
Sbjct: 536  IPMSSGATGSGKEGEDASVNQDGNGNLDF-SNNAFDLEAISL-IDPTYGFGNENPSVPAG 593

Query: 1375 LEEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGNG---I 1205
              EVI+LSDSEEENEP+ISSG ++ NNH D     FP    GIP S+ +      G   +
Sbjct: 594  DAEVIVLSDSEEENEPIISSGPIFNNNHSDAPVVPFPCQSQGIPDSFHDSTLVNGGNSCL 653

Query: 1204 DLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGYTLT 1025
             LFN++DD+FGM+MWSLPSG+QG PGFQLF SDADV+ +LVD QHGSINCP++++GY L 
Sbjct: 654  GLFNSNDDEFGMNMWSLPSGTQGGPGFQLFSSDADVSGSLVDEQHGSINCPSSLSGYGLA 713

Query: 1024 AETAMGSAALVPESSVK--HTNVNDGLVDNPLAFSGNDPSLQIFLPTRPSDASAQADMRD 851
            A+T +GS +L+P +     + N+ND LVDN LAFSGNDPSLQIFLPTRPSD S +A  RD
Sbjct: 714  ADTGIGSTSLLPGTYANRPNANINDSLVDNSLAFSGNDPSLQIFLPTRPSDTSVEA-TRD 772

Query: 850  LPDVSNGIRXVEGVMVSL 797
             PDVSNG+   + + + L
Sbjct: 773  QPDVSNGVGTEDWISLRL 790



 Score = 88.2 bits (217), Expect = 4e-14
 Identities = 48/78 (61%), Positives = 55/78 (70%)
 Frame = -2

Query: 818  GGGHGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXGMNDNKSGKASRERSDSPFSFPR 639
            GG HG+  A N LSSG Q+Q+K               GMND++  K+SRERSDSPF+FPR
Sbjct: 794  GGVHGDSVA-NALSSGQQVQTKDTALDSLADTASLLLGMNDSRPTKSSRERSDSPFTFPR 852

Query: 638  QRRSVRPRLYLSIDSDSE 585
            QRRSVRPRLYLSIDSDSE
Sbjct: 853  QRRSVRPRLYLSIDSDSE 870


>ref|XP_009617745.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isoform X1 [Nicotiana
            tomentosiformis] gi|697127401|ref|XP_009617746.1|
            PREDICTED: E3 SUMO-protein ligase SIZ1 isoform X1
            [Nicotiana tomentosiformis]
            gi|697127403|ref|XP_009617747.1| PREDICTED: E3
            SUMO-protein ligase SIZ1 isoform X1 [Nicotiana
            tomentosiformis]
          Length = 878

 Score = 1142 bits (2953), Expect = 0.0
 Identities = 563/798 (70%), Positives = 665/798 (83%), Gaps = 8/798 (1%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLV SCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILA LSD+RVSGMW KKN+V
Sbjct: 1    MDLVASCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILATLSDERVSGMWPKKNSV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLCGSTL 2807
            GKE+VAKLVDD YRKMQ+ GAT+LASKS   +   NVK KEE E+     KIRC+C S+L
Sbjct: 61   GKEDVAKLVDDIYRKMQVGGATELASKSQVVSDSSNVKLKEEIEDSSYHMKIRCVCASSL 120

Query: 2806 PTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEI-FYCEICRLSRADPFWMTVAH 2630
             T++MI+CED RC+ WQH+ CV+IP+KP+EG  P  P   FYCE+CRL RADPFW+T+ H
Sbjct: 121  QTETMIQCEDRRCHTWQHVRCVIIPDKPMEGGDPPIPPTTFYCELCRLGRADPFWVTMGH 180

Query: 2629 PLYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQ 2450
            PLYP KL+ITSVP+DG+NPVQSIEKTFQ+TRAD+DLL+KQEYD+QAWCMLLNDKV FRMQ
Sbjct: 181  PLYPAKLSITSVPADGTNPVQSIEKTFQITRADRDLLAKQEYDLQAWCMLLNDKVQFRMQ 240

Query: 2449 WPLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLG 2270
            WP YADLQ+NGVPVRAINRPGSQ LGANGRDDGP ITPCTRDGINKI+LTGCDAR+FCLG
Sbjct: 241  WPQYADLQVNGVPVRAINRPGSQLLGANGRDDGPIITPCTRDGINKITLTGCDARVFCLG 300

Query: 2269 VRIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPV 2090
            VR+VKRRT+ QVL++IPKES+GE+FEDAL RVRRCVGGG ATENADSDSD+EVVAD IPV
Sbjct: 301  VRLVKRRTVQQVLSLIPKESDGEQFEDALTRVRRCVGGGTATENADSDSDLEVVADCIPV 360

Query: 2089 NLRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPY 1910
            NLRCPMSG RMKVAGRF+PC+HMGCFDLEVFVEMN RSRKWQCPICLKNY+LE +IIDPY
Sbjct: 361  NLRCPMSGSRMKVAGRFKPCIHMGCFDLEVFVEMNQRSRKWQCPICLKNYALEHVIIDPY 420

Query: 1909 FNRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEGESKP 1730
            FNRITS++R+CGEDV EIEVKPDGSWRAK EGDR++LG+LG WHLPDG+L  S++ ESKP
Sbjct: 421  FNRITSRLRSCGEDVAEIEVKPDGSWRAKIEGDRQSLGDLGRWHLPDGSLSESLDIESKP 480

Query: 1729 KQEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFENDDHNV 1550
            K E +KQIKQEGGSD + GLK+G+++NRNG WE++K +D    SS SRL+++F  D   +
Sbjct: 481  KPEILKQIKQEGGSDGN-GLKVGLRKNRNGLWEISKPED-QTFSSGSRLRDNFGQD---I 535

Query: 1549 IPMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQN--LPAP 1376
            IPMSS ATGSG++GEDASVNQDG  NLDF +NN  +LE+ISL +D +YGF  +N  +PA 
Sbjct: 536  IPMSSGATGSGKEGEDASVNQDGNGNLDF-SNNAFDLEAISL-IDPTYGFGNENPSVPAG 593

Query: 1375 LEEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGNG---I 1205
              EVI+LSDSEEENEP+ISSG ++ NNH D     FP    GIP S+ +      G   +
Sbjct: 594  DAEVIVLSDSEEENEPIISSGPIFNNNHSDAPVVPFPCQSQGIPDSFHDSTLVNGGNSCL 653

Query: 1204 DLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGYTLT 1025
             LFN++DD+FGM+MWSLPSG+QG PGFQLF SDADV+ +LVD QHGSINCP++++GY L 
Sbjct: 654  GLFNSNDDEFGMNMWSLPSGTQGGPGFQLFSSDADVSGSLVDEQHGSINCPSSLSGYGLA 713

Query: 1024 AETAMGSAALVPESSVK--HTNVNDGLVDNPLAFSGNDPSLQIFLPTRPSDASAQADMRD 851
            A+T +GS +L+P +     + N+ND LVDN LAFSGNDPSLQIFLPTRPSD S +A  RD
Sbjct: 714  ADTGIGSTSLLPGTYANRPNANINDSLVDNSLAFSGNDPSLQIFLPTRPSDTSVEA-TRD 772

Query: 850  LPDVSNGIRXVEGVMVSL 797
             PDVSNG+   + + + L
Sbjct: 773  QPDVSNGVGTEDWISLRL 790



 Score = 80.9 bits (198), Expect = 6e-12
 Identities = 47/86 (54%), Positives = 54/86 (62%), Gaps = 8/86 (9%)
 Frame = -2

Query: 818  GGGHGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXG--------MNDNKSGKASRERS 663
            GG HG+  A N LSSG Q+Q+K                        MND++  K+SRERS
Sbjct: 794  GGVHGDSVA-NALSSGQQVQTKDTALDSLADTGSDATVCAASLLLGMNDSRPTKSSRERS 852

Query: 662  DSPFSFPRQRRSVRPRLYLSIDSDSE 585
            DSPF+FPRQRRSVRPRLYLSIDSDSE
Sbjct: 853  DSPFTFPRQRRSVRPRLYLSIDSDSE 878


>ref|XP_010651134.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isoform X2 [Vitis vinifera]
          Length = 852

 Score = 1137 bits (2941), Expect = 0.0
 Identities = 567/801 (70%), Positives = 653/801 (81%), Gaps = 11/801 (1%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSD++VS MWAKKNAV
Sbjct: 1    MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDEQVSRMWAKKNAV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLCGSTL 2807
            GKEEVAKLV+DTYRKMQ+SGATDLASK    +   NVK KEE E+ Y   KIRC CGS L
Sbjct: 61   GKEEVAKLVEDTYRKMQVSGATDLASKGQVLSDSSNVKFKEELEDSYNDMKIRCPCGSAL 120

Query: 2806 PTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEIFYCEICRLSRADPFWMTVAHP 2627
            P ++M+KC+D +C VWQHI CV+IPEK +EG+ P TP+ FYCEICRLSRADPFW+TVAHP
Sbjct: 121  PNETMLKCDDLKCQVWQHIGCVIIPEKTMEGI-PPTPDPFYCEICRLSRADPFWVTVAHP 179

Query: 2626 LYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQW 2447
            L PVKLT TS+P+DG+NPVQS+EKTF LTRAD+D++SK EYDVQAWC+LLNDKV FRMQW
Sbjct: 180  LLPVKLTTTSIPTDGTNPVQSVEKTFHLTRADRDMVSKHEYDVQAWCILLNDKVSFRMQW 239

Query: 2446 PLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLGV 2267
            P YADLQ+NG+ VRAINRPGSQ LGANGRDDGP ITPCT+DGINKISLTGCDARIFCLGV
Sbjct: 240  PQYADLQVNGMAVRAINRPGSQLLGANGRDDGPVITPCTKDGINKISLTGCDARIFCLGV 299

Query: 2266 RIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPVN 2087
            RIVKRRT+ Q+L++IPKES+GERFEDALARVRRC+GGG AT+NADSDSD+EVVADF  VN
Sbjct: 300  RIVKRRTVQQILSLIPKESDGERFEDALARVRRCIGGGGATDNADSDSDLEVVADFFTVN 359

Query: 2086 LRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPYF 1907
            LRCPMSG RMKVAGRF+PC HMGCFDLE+FVEMN RSRKWQCPICLKNYSLE +IIDPYF
Sbjct: 360  LRCPMSGSRMKVAGRFKPCAHMGCFDLEIFVEMNQRSRKWQCPICLKNYSLENVIIDPYF 419

Query: 1906 NRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEGESKPK 1727
            NRITS +++CGEDV EI+VKPDG WR K E +R   G L  WH  DG LCP  EGE KPK
Sbjct: 420  NRITSSMQSCGEDVTEIQVKPDGCWRVKPENER---GILAQWHNADGTLCPLAEGEFKPK 476

Query: 1726 QEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFENDDHNVI 1547
             + +KQIKQEG S+ H+ LKL +K NRNG WE++K D+ +   + +RLQE FE+    VI
Sbjct: 477  MDVLKQIKQEGISECHSSLKLQIK-NRNGVWEVSKPDE-MNTLTCNRLQEKFEDPGQQVI 534

Query: 1546 PMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMD-QSYGFNEQNLPAPL- 1373
            PMSSSATGSGRDGED SVNQDG  N DF+TN GIEL+SISLN+D  +Y F E+N PAP+ 
Sbjct: 535  PMSSSATGSGRDGEDPSVNQDGGGNYDFSTNPGIELDSISLNIDNNAYAFPERNTPAPMG 594

Query: 1372 -EEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGNG---- 1208
              E+I+LSDSEEEN+ L+SSG +Y N+  D GG  F I   GIP SY EDP  G G    
Sbjct: 595  DTELIVLSDSEEENDTLMSSGTLYNNSRADAGGINFSIPT-GIPDSYAEDPTAGPGGSSC 653

Query: 1207 IDLFNAHDDDFGM--HMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGY 1034
            + LF+  DDDFGM   +W LP G+Q  PGFQ FG+D DV+DAL D+QH  INCPT++NGY
Sbjct: 654  LGLFSTADDDFGMSGSLWPLPPGTQPGPGFQFFGTDTDVSDALADLQHNPINCPTSMNGY 713

Query: 1033 TLTAETAMGSAALVPESSV--KHTNVNDGLVDNPLAFSGNDPSLQIFLPTRPSDASAQAD 860
            TL  E  MGSAALVP+ S+    T++NDGLVDNPLAF G+DPSLQIFLPTRPSDAS   D
Sbjct: 714  TLGPEVVMGSAALVPDPSIGRTDTDMNDGLVDNPLAFGGDDPSLQIFLPTRPSDASVPTD 773

Query: 859  MRDLPDVSNGIRXVEGVMVSL 797
            +R+  DVSNG R  + + + L
Sbjct: 774  LRNQADVSNGSRPDDWISLRL 794


>ref|XP_010651133.1| PREDICTED: E3 SUMO-protein ligase SIZ1 isoform X1 [Vitis vinifera]
          Length = 876

 Score = 1137 bits (2941), Expect = 0.0
 Identities = 567/801 (70%), Positives = 653/801 (81%), Gaps = 11/801 (1%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSD++VS MWAKKNAV
Sbjct: 1    MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDEQVSRMWAKKNAV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLCGSTL 2807
            GKEEVAKLV+DTYRKMQ+SGATDLASK    +   NVK KEE E+ Y   KIRC CGS L
Sbjct: 61   GKEEVAKLVEDTYRKMQVSGATDLASKGQVLSDSSNVKFKEELEDSYNDMKIRCPCGSAL 120

Query: 2806 PTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEIFYCEICRLSRADPFWMTVAHP 2627
            P ++M+KC+D +C VWQHI CV+IPEK +EG+ P TP+ FYCEICRLSRADPFW+TVAHP
Sbjct: 121  PNETMLKCDDLKCQVWQHIGCVIIPEKTMEGI-PPTPDPFYCEICRLSRADPFWVTVAHP 179

Query: 2626 LYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQW 2447
            L PVKLT TS+P+DG+NPVQS+EKTF LTRAD+D++SK EYDVQAWC+LLNDKV FRMQW
Sbjct: 180  LLPVKLTTTSIPTDGTNPVQSVEKTFHLTRADRDMVSKHEYDVQAWCILLNDKVSFRMQW 239

Query: 2446 PLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLGV 2267
            P YADLQ+NG+ VRAINRPGSQ LGANGRDDGP ITPCT+DGINKISLTGCDARIFCLGV
Sbjct: 240  PQYADLQVNGMAVRAINRPGSQLLGANGRDDGPVITPCTKDGINKISLTGCDARIFCLGV 299

Query: 2266 RIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPVN 2087
            RIVKRRT+ Q+L++IPKES+GERFEDALARVRRC+GGG AT+NADSDSD+EVVADF  VN
Sbjct: 300  RIVKRRTVQQILSLIPKESDGERFEDALARVRRCIGGGGATDNADSDSDLEVVADFFTVN 359

Query: 2086 LRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPYF 1907
            LRCPMSG RMKVAGRF+PC HMGCFDLE+FVEMN RSRKWQCPICLKNYSLE +IIDPYF
Sbjct: 360  LRCPMSGSRMKVAGRFKPCAHMGCFDLEIFVEMNQRSRKWQCPICLKNYSLENVIIDPYF 419

Query: 1906 NRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEGESKPK 1727
            NRITS +++CGEDV EI+VKPDG WR K E +R   G L  WH  DG LCP  EGE KPK
Sbjct: 420  NRITSSMQSCGEDVTEIQVKPDGCWRVKPENER---GILAQWHNADGTLCPLAEGEFKPK 476

Query: 1726 QEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFENDDHNVI 1547
             + +KQIKQEG S+ H+ LKL +K NRNG WE++K D+ +   + +RLQE FE+    VI
Sbjct: 477  MDVLKQIKQEGISECHSSLKLQIK-NRNGVWEVSKPDE-MNTLTCNRLQEKFEDPGQQVI 534

Query: 1546 PMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMD-QSYGFNEQNLPAPL- 1373
            PMSSSATGSGRDGED SVNQDG  N DF+TN GIEL+SISLN+D  +Y F E+N PAP+ 
Sbjct: 535  PMSSSATGSGRDGEDPSVNQDGGGNYDFSTNPGIELDSISLNIDNNAYAFPERNTPAPMG 594

Query: 1372 -EEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGNG---- 1208
              E+I+LSDSEEEN+ L+SSG +Y N+  D GG  F I   GIP SY EDP  G G    
Sbjct: 595  DTELIVLSDSEEENDTLMSSGTLYNNSRADAGGINFSIPT-GIPDSYAEDPTAGPGGSSC 653

Query: 1207 IDLFNAHDDDFGM--HMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGY 1034
            + LF+  DDDFGM   +W LP G+Q  PGFQ FG+D DV+DAL D+QH  INCPT++NGY
Sbjct: 654  LGLFSTADDDFGMSGSLWPLPPGTQPGPGFQFFGTDTDVSDALADLQHNPINCPTSMNGY 713

Query: 1033 TLTAETAMGSAALVPESSV--KHTNVNDGLVDNPLAFSGNDPSLQIFLPTRPSDASAQAD 860
            TL  E  MGSAALVP+ S+    T++NDGLVDNPLAF G+DPSLQIFLPTRPSDAS   D
Sbjct: 714  TLGPEVVMGSAALVPDPSIGRTDTDMNDGLVDNPLAFGGDDPSLQIFLPTRPSDASVPTD 773

Query: 859  MRDLPDVSNGIRXVEGVMVSL 797
            +R+  DVSNG R  + + + L
Sbjct: 774  LRNQADVSNGSRPDDWISLRL 794



 Score = 84.0 bits (206), Expect = 7e-13
 Identities = 53/93 (56%), Positives = 59/93 (63%), Gaps = 5/93 (5%)
 Frame = -2

Query: 848  SRCFKWYSX--GG--GGHGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXGMNDNKSGK 681
            SR   W S   GG  GGH E  A+N L++  QL SK               GMND +S K
Sbjct: 784  SRPDDWISLRLGGSSGGHAESPAANGLNTRQQLPSKDGDMDSLADTASLLLGMNDGRSDK 843

Query: 680  -ASRERSDSPFSFPRQRRSVRPRLYLSIDSDSE 585
             +SR+RSDSPFSFPRQRRSVRPRLYLSIDSDSE
Sbjct: 844  TSSRQRSDSPFSFPRQRRSVRPRLYLSIDSDSE 876


>ref|XP_009601717.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like isoform X2 [Nicotiana
            tomentosiformis]
          Length = 883

 Score = 1135 bits (2937), Expect = 0.0
 Identities = 560/799 (70%), Positives = 657/799 (82%), Gaps = 9/799 (1%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDL+TSCKDKLA+FR+KELKDVLTQLGLSKQGKKQDLVDRILAILSD++VS MWAKKN+V
Sbjct: 1    MDLLTSCKDKLAHFRVKELKDVLTQLGLSKQGKKQDLVDRILAILSDEQVSVMWAKKNSV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLCGSTL 2807
            GKEEVAKLVD  YRKMQ+SGATDLASKS   +   NVK KEE E+ YQM KIRC CGS+L
Sbjct: 61   GKEEVAKLVDGIYRKMQVSGATDLASKSQVVSDSSNVKLKEEIEDTYQM-KIRCPCGSSL 119

Query: 2806 PTDSMIKCEDPRCNVWQHIACVVIPEKPVEG-VLPNTPEIFYCEICRLSRADPFWMTVAH 2630
             T++MI+CE+ RC  WQH++CVV+PEKP+EG V P  PE FYCE+CRLSRADPF +T+A+
Sbjct: 120  QTETMIQCENRRCRTWQHVSCVVVPEKPMEGGVPPIPPETFYCELCRLSRADPFLVTMAN 179

Query: 2629 PLYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQ 2450
            PLYP KL ITSVP DG++P+Q IEKTFQLTRAD+DLL KQEYDVQ WCMLLNDKV FRMQ
Sbjct: 180  PLYPAKLAITSVPVDGTSPIQRIEKTFQLTRADRDLLLKQEYDVQVWCMLLNDKVQFRMQ 239

Query: 2449 WPLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLG 2270
            WP YADLQ+NG+PVR INRPGSQ LGANGRDDGP ITPC+RDGINKI LTGCDAR+FCLG
Sbjct: 240  WPQYADLQVNGIPVRCINRPGSQLLGANGRDDGPIITPCSRDGINKIILTGCDARVFCLG 299

Query: 2269 VRIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPV 2090
            VR+V RRT  QVLN+IPKE++GE F+DALARVRRCVGGG ATENADSDSD+EVVADFIPV
Sbjct: 300  VRLVNRRTFQQVLNVIPKEADGEVFDDALARVRRCVGGGTATENADSDSDLEVVADFIPV 359

Query: 2089 NLRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPY 1910
            NLRCPMSG RMKVAGRF+PCVHMGCFDLEVFVEMN RSRKWQCPICLKNYSLE +IIDPY
Sbjct: 360  NLRCPMSGSRMKVAGRFKPCVHMGCFDLEVFVEMNQRSRKWQCPICLKNYSLEHVIIDPY 419

Query: 1909 FNRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEGESKP 1730
            FN+ITS++R CGE+V EIEVKPDGSWRAK EGDRR LG+LG WHLPDGNL  S + E K 
Sbjct: 420  FNQITSQLRTCGEEVTEIEVKPDGSWRAKAEGDRRNLGDLGRWHLPDGNLIESQDIEPKA 479

Query: 1729 KQEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFENDDHNV 1550
            K   +K +KQEGGS+SH+GLK+G+K+NRNG WE++K +D+  +   + ++E+FEN   ++
Sbjct: 480  KPGILKHVKQEGGSESHSGLKVGLKKNRNGLWEISKPEDMQTLPYENSVRENFENQIQDI 539

Query: 1549 IPMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQNLPAPL- 1373
            I MSSSATGSG++GED SVNQDG  N D  +NNG ELE+IS N    YGFN +N PAP  
Sbjct: 540  IAMSSSATGSGKEGEDLSVNQDGDVNFD-NSNNGFELETISPNFGPRYGFNGRNPPAPSG 598

Query: 1372 -EEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGNG---- 1208
              EVI+LSDS+ ENEPLISS +++ NNH D    +FP    GI  S  ++ A  N     
Sbjct: 599  DAEVIVLSDSDGENEPLISSTSIHSNNHADASIVSFPGRPKGISDSCHDNHAVVNDGNSC 658

Query: 1207 IDLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGYTL 1028
            + LFNA+DD+FGM MW LPS +QG P FQLFGSD DV+ +LVD+QHGSINCP+++NGY+L
Sbjct: 659  LGLFNANDDEFGMDMWPLPSVNQGGPSFQLFGSDTDVSGSLVDMQHGSINCPSSINGYSL 718

Query: 1027 TAETAMGSAALVPESSVKHTN--VNDGLVDNPLAFSGNDPSLQIFLPTRPSDASAQADMR 854
             A+T +GS +L+PESSV   N  +NDGLV+N L+F GNDPSLQIFLPTRPSDAS +AD R
Sbjct: 719  AADTGVGSCSLLPESSVDRLNAEINDGLVNNSLSFGGNDPSLQIFLPTRPSDASVEADTR 778

Query: 853  DLPDVSNGIRXVEGVMVSL 797
              P V NGI   + + +SL
Sbjct: 779  HRPGVRNGIHTEDWISLSL 797



 Score = 77.8 bits (190), Expect = 5e-11
 Identities = 46/92 (50%), Positives = 52/92 (56%), Gaps = 9/92 (9%)
 Frame = -2

Query: 833  WYSXGGGGHGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXGMNDNKS---------GK 681
            W S   G  G+ A +N LSSG   Q+K               GMND  S         G 
Sbjct: 792  WISLSLGAGGDSAVANELSSGQPSQTKNSSLDSVADTASLLLGMNDGVSMKSSKERSNGP 851

Query: 680  ASRERSDSPFSFPRQRRSVRPRLYLSIDSDSE 585
            +SRERSD PF+FPRQRRSVRPRLYLSIDSD+E
Sbjct: 852  SSRERSDGPFNFPRQRRSVRPRLYLSIDSDTE 883


>ref|XP_002526365.1| sumo ligase, putative [Ricinus communis] gi|223534324|gb|EEF36036.1|
            sumo ligase, putative [Ricinus communis]
          Length = 876

 Score = 1134 bits (2932), Expect = 0.0
 Identities = 559/799 (69%), Positives = 653/799 (81%), Gaps = 9/799 (1%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILA+L+D++V    AKK+ V
Sbjct: 1    MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAVLTDEQVPKTSAKKSVV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMD-KIRCLCGST 2810
            GKEEVAKLVDD YRKMQ+SGATDLASK +          K E ++ +  D K+RC CGS+
Sbjct: 61   GKEEVAKLVDDIYRKMQVSGATDLASKGEGVLESSKPVIKGEIDDSFHFDTKVRCPCGSS 120

Query: 2809 LPTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEIFYCEICRLSRADPFWMTVAH 2630
            L T+SMIKCEDPRC VWQHI CV+IPEKP+E + P  P++FYCEICRL RADPFW++VAH
Sbjct: 121  LETESMIKCEDPRCRVWQHIGCVIIPEKPMEAI-PQVPDLFYCEICRLCRADPFWVSVAH 179

Query: 2629 PLYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVPFRMQ 2450
            PLYPVKLT T++ +DGS PVQS EKTF LTRADKDLL+KQEYDVQAWCMLLNDKVPFRMQ
Sbjct: 180  PLYPVKLT-TNIQADGSTPVQSAEKTFHLTRADKDLLAKQEYDVQAWCMLLNDKVPFRMQ 238

Query: 2449 WPLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARIFCLG 2270
            WP YADLQ+NGVPVRAINRPGSQ LG NGRDDGP ITPCT+DGINKISL GCDARIFCLG
Sbjct: 239  WPQYADLQVNGVPVRAINRPGSQLLGINGRDDGPIITPCTKDGINKISLNGCDARIFCLG 298

Query: 2269 VRIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVADFIPV 2090
            VRIVKRRT+ Q+LN+IPKES+GERFEDALARV RCVGGGAA +NADSDSD+EVVAD   V
Sbjct: 299  VRIVKRRTVQQILNMIPKESDGERFEDALARVCRCVGGGAA-DNADSDSDLEVVADSFAV 357

Query: 2089 NLRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKIIIDPY 1910
            NLRCPMSG RMKVAGRF+PC HMGCFDLEVF+EMN RSRKWQCP+CLKNYSLE +IIDPY
Sbjct: 358  NLRCPMSGSRMKVAGRFKPCAHMGCFDLEVFLEMNQRSRKWQCPVCLKNYSLENVIIDPY 417

Query: 1909 FNRITSKIRNCGEDVNEIEVKPDGSWRAKT--EGDRRALGELGLWHLPDGNLCPSMEGES 1736
            FNR+TSK+++CGED+ EIEVKPDGSWRAKT  E +RR +GEL  WH PDG+LC  + GE 
Sbjct: 418  FNRVTSKMQHCGEDITEIEVKPDGSWRAKTKSEAERRDVGELAQWHNPDGSLCVPISGEH 477

Query: 1735 KPKQEFVKQIKQEGGSDSH--AGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFEND 1562
            K K E  KQIKQEG S+ +   GLKLG+++NRNG WE++K +D+   SS +RL E FE  
Sbjct: 478  KSKVEMEKQIKQEGNSEGYNGTGLKLGIRKNRNGFWEVSKPEDVNTSSSGNRLPERFEII 537

Query: 1561 DHNVIPMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQNLP 1382
            +  VIPMSSSATGSGRDGED SVNQDG  N DF TNNGIEL+S+ LN+D +YGF ++N  
Sbjct: 538  EQKVIPMSSSATGSGRDGEDPSVNQDGGGNFDF-TNNGIELDSLPLNVDSTYGFPDRNFS 596

Query: 1381 APLE--EVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGNG 1208
            AP+E  EVI+LSDS+++N+ L+++G VYKN+  D+GG  F +  +GI   YPEDP  GNG
Sbjct: 597  APVEDPEVIVLSDSDDDNDILMTTGTVYKNSQTDDGGAGFSMPPNGISNPYPEDPTVGNG 656

Query: 1207 IDLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVNGYTL 1028
            +   N +DD+FG+ +W LP GSQ  PGFQLF S  DV DALVD+QHG I+CP T+NGYTL
Sbjct: 657  LGFLNPNDDEFGIPLWPLPPGSQAGPGFQLFNS--DVPDALVDIQHGPISCPMTINGYTL 714

Query: 1027 TAETAMGSAALVPESSV--KHTNVNDGLVDNPLAFSGNDPSLQIFLPTRPSDASAQADMR 854
              ET MG ++LV +SSV    T+ NDGLV+NPLAF G DPSLQIFLPTRPSDAS Q+D+R
Sbjct: 715  APETVMGPSSLVADSSVGRSDTDTNDGLVNNPLAFGGEDPSLQIFLPTRPSDASGQSDLR 774

Query: 853  DLPDVSNGIRXVEGVMVSL 797
            D  DVSNG+R  + + + L
Sbjct: 775  DQADVSNGVRTEDWISLRL 793



 Score = 81.3 bits (199), Expect = 5e-12
 Identities = 43/83 (51%), Positives = 54/83 (65%), Gaps = 4/83 (4%)
 Frame = -2

Query: 821  GGGG----HGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXGMNDNKSGKASRERSDSP 654
            GGGG    HG+  ++N ++S  Q+  +               GMND +S KASR+RSDSP
Sbjct: 794  GGGGATGSHGDSVSANGVNSRQQMPPRDGAMDSLADTASLLLGMNDGRSEKASRQRSDSP 853

Query: 653  FSFPRQRRSVRPRLYLSIDSDSE 585
            F FPRQ+RS+RPRLYLSIDSDSE
Sbjct: 854  FQFPRQKRSIRPRLYLSIDSDSE 876


>ref|XP_009601705.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like isoform X1 [Nicotiana
            tomentosiformis]
          Length = 887

 Score = 1130 bits (2922), Expect = 0.0
 Identities = 560/803 (69%), Positives = 657/803 (81%), Gaps = 13/803 (1%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDR----VSGMWAK 2999
            MDL+TSCKDKLA+FR+KELKDVLTQLGLSKQGKKQDLVDRILAILSD++    VS MWAK
Sbjct: 1    MDLLTSCKDKLAHFRVKELKDVLTQLGLSKQGKKQDLVDRILAILSDEQGEQPVSVMWAK 60

Query: 2998 KNAVGKEEVAKLVDDTYRKMQISGATDLASKSDSSNVKLNVKPKEETEEPYQMDKIRCLC 2819
            KN+VGKEEVAKLVD  YRKMQ+SGATDLASKS   +   NVK KEE E+ YQM KIRC C
Sbjct: 61   KNSVGKEEVAKLVDGIYRKMQVSGATDLASKSQVVSDSSNVKLKEEIEDTYQM-KIRCPC 119

Query: 2818 GSTLPTDSMIKCEDPRCNVWQHIACVVIPEKPVEG-VLPNTPEIFYCEICRLSRADPFWM 2642
            GS+L T++MI+CE+ RC  WQH++CVV+PEKP+EG V P  PE FYCE+CRLSRADPF +
Sbjct: 120  GSSLQTETMIQCENRRCRTWQHVSCVVVPEKPMEGGVPPIPPETFYCELCRLSRADPFLV 179

Query: 2641 TVAHPLYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDKVP 2462
            T+A+PLYP KL ITSVP DG++P+Q IEKTFQLTRAD+DLL KQEYDVQ WCMLLNDKV 
Sbjct: 180  TMANPLYPAKLAITSVPVDGTSPIQRIEKTFQLTRADRDLLLKQEYDVQVWCMLLNDKVQ 239

Query: 2461 FRMQWPLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDARI 2282
            FRMQWP YADLQ+NG+PVR INRPGSQ LGANGRDDGP ITPC+RDGINKI LTGCDAR+
Sbjct: 240  FRMQWPQYADLQVNGIPVRCINRPGSQLLGANGRDDGPIITPCSRDGINKIILTGCDARV 299

Query: 2281 FCLGVRIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVVAD 2102
            FCLGVR+V RRT  QVLN+IPKE++GE F+DALARVRRCVGGG ATENADSDSD+EVVAD
Sbjct: 300  FCLGVRLVNRRTFQQVLNVIPKEADGEVFDDALARVRRCVGGGTATENADSDSDLEVVAD 359

Query: 2101 FIPVNLRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEKII 1922
            FIPVNLRCPMSG RMKVAGRF+PCVHMGCFDLEVFVEMN RSRKWQCPICLKNYSLE +I
Sbjct: 360  FIPVNLRCPMSGSRMKVAGRFKPCVHMGCFDLEVFVEMNQRSRKWQCPICLKNYSLEHVI 419

Query: 1921 IDPYFNRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSMEG 1742
            IDPYFN+ITS++R CGE+V EIEVKPDGSWRAK EGDRR LG+LG WHLPDGNL  S + 
Sbjct: 420  IDPYFNQITSQLRTCGEEVTEIEVKPDGSWRAKAEGDRRNLGDLGRWHLPDGNLIESQDI 479

Query: 1741 ESKPKQEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFEND 1562
            E K K   +K +KQEGGS+SH+GLK+G+K+NRNG WE++K +D+  +   + ++E+FEN 
Sbjct: 480  EPKAKPGILKHVKQEGGSESHSGLKVGLKKNRNGLWEISKPEDMQTLPYENSVRENFENQ 539

Query: 1561 DHNVIPMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQNLP 1382
              ++I MSSSATGSG++GED SVNQDG  N D  +NNG ELE+IS N    YGFN +N P
Sbjct: 540  IQDIIAMSSSATGSGKEGEDLSVNQDGDVNFD-NSNNGFELETISPNFGPRYGFNGRNPP 598

Query: 1381 APL--EEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFGNG 1208
            AP    EVI+LSDS+ ENEPLISS +++ NNH D    +FP    GI  S  ++ A  N 
Sbjct: 599  APSGDAEVIVLSDSDGENEPLISSTSIHSNNHADASIVSFPGRPKGISDSCHDNHAVVND 658

Query: 1207 ----IDLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTTVN 1040
                + LFNA+DD+FGM MW LPS +QG P FQLFGSD DV+ +LVD+QHGSINCP+++N
Sbjct: 659  GNSCLGLFNANDDEFGMDMWPLPSVNQGGPSFQLFGSDTDVSGSLVDMQHGSINCPSSIN 718

Query: 1039 GYTLTAETAMGSAALVPESSVKHTN--VNDGLVDNPLAFSGNDPSLQIFLPTRPSDASAQ 866
            GY+L A+T +GS +L+PESSV   N  +NDGLV+N L+F GNDPSLQIFLPTRPSDAS +
Sbjct: 719  GYSLAADTGVGSCSLLPESSVDRLNAEINDGLVNNSLSFGGNDPSLQIFLPTRPSDASVE 778

Query: 865  ADMRDLPDVSNGIRXVEGVMVSL 797
            AD R  P V NGI   + + +SL
Sbjct: 779  ADTRHRPGVRNGIHTEDWISLSL 801



 Score = 77.8 bits (190), Expect = 5e-11
 Identities = 46/92 (50%), Positives = 52/92 (56%), Gaps = 9/92 (9%)
 Frame = -2

Query: 833  WYSXGGGGHGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXGMNDNKS---------GK 681
            W S   G  G+ A +N LSSG   Q+K               GMND  S         G 
Sbjct: 796  WISLSLGAGGDSAVANELSSGQPSQTKNSSLDSVADTASLLLGMNDGVSMKSSKERSNGP 855

Query: 680  ASRERSDSPFSFPRQRRSVRPRLYLSIDSDSE 585
            +SRERSD PF+FPRQRRSVRPRLYLSIDSD+E
Sbjct: 856  SSRERSDGPFNFPRQRRSVRPRLYLSIDSDTE 887


>ref|XP_006340142.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like isoform X3 [Solanum
            tuberosum]
          Length = 877

 Score = 1127 bits (2916), Expect = 0.0
 Identities = 566/805 (70%), Positives = 665/805 (82%), Gaps = 15/805 (1%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLV SCKDKLAYFRIKELKDVL QLGLSKQGKKQDLVDRI+A LSD+R SG++ K+N+V
Sbjct: 1    MDLVASCKDKLAYFRIKELKDVLNQLGLSKQGKKQDLVDRIIATLSDERASGLFPKRNSV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGAT------DLASKSDSSNVKLNVKPKEETEEPYQMDKIRC 2825
            GKE+VAKLVDD YRKMQ+SGAT      DLASKS   +   NVK KEE E+ Y M KIRC
Sbjct: 61   GKEDVAKLVDDIYRKMQVSGATGATGATDLASKSQVVSDTSNVKLKEEIEDTYHM-KIRC 119

Query: 2824 LCGSTLPTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEI-FYCEICRLSRADPF 2648
            +C S+L T++MI+CED RC+ WQHI CVVIP+KP+EG  P  P   FYCE+CRL RADPF
Sbjct: 120  VCTSSLQTETMIQCEDRRCHTWQHIRCVVIPDKPMEGGDPPIPPTTFYCEVCRLVRADPF 179

Query: 2647 WMTVAHPLYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDK 2468
            W+T+ HPLYP KL ITSVP+DG+NPVQSIEKTFQ+TRAD+DLL+KQEYD+QAWCMLLNDK
Sbjct: 180  WVTMGHPLYPAKLAITSVPADGTNPVQSIEKTFQITRADRDLLAKQEYDLQAWCMLLNDK 239

Query: 2467 VPFRMQWPLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDA 2288
            V FRMQWP YADLQ+NGVPVRAINRPGSQ LGANGRDDGP ITPCTRDGINK++LTGCDA
Sbjct: 240  VQFRMQWPQYADLQVNGVPVRAINRPGSQLLGANGRDDGPIITPCTRDGINKVTLTGCDA 299

Query: 2287 RIFCLGVRIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVV 2108
            R+FCLGVR+VKRRT+ QVL++IPK S+GE+FEDALARVRRCVGGG ATENADSDSD+EVV
Sbjct: 300  RVFCLGVRLVKRRTVQQVLSMIPKVSDGEKFEDALARVRRCVGGGTATENADSDSDLEVV 359

Query: 2107 ADFIPVNLRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEK 1928
            AD IPVNLRCPMSG RMKVAGRF+PC+HMGCFDL+VFVEMN RSRKWQCPICLKNYSLE 
Sbjct: 360  ADCIPVNLRCPMSGSRMKVAGRFKPCIHMGCFDLDVFVEMNQRSRKWQCPICLKNYSLEH 419

Query: 1927 IIIDPYFNRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSM 1748
            +IIDPYFNRITS++R+CGEDV EIEVKPDGSWRAKTE DRR+LG+LG WHLPDG+L  S 
Sbjct: 420  VIIDPYFNRITSQMRSCGEDVTEIEVKPDGSWRAKTESDRRSLGDLGRWHLPDGSLSESP 479

Query: 1747 EGESKPKQEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFE 1568
            + ESKPK E +KQ+KQEGGSD + GLK+G+K+NR+G WE++K +D    SS +RL+E+F 
Sbjct: 480  DIESKPKPEILKQVKQEGGSDGN-GLKVGLKKNRDGLWEISKPED-QTFSSGNRLRENFG 537

Query: 1567 NDDHNVIPMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQN 1388
             D   VIPMSSSATGSG++GED SVNQDG  NL+F +NN  +LE+ISLN+D  YGF   N
Sbjct: 538  QD---VIPMSSSATGSGKEGEDRSVNQDGNGNLEF-SNNAFDLEAISLNIDPPYGFGHGN 593

Query: 1387 --LPAPLEEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFG 1214
              +PA   EVI+LSDSEEENEP+I SGAV+ NNH D    +FP    GIP S+  D A  
Sbjct: 594  PSVPAGDAEVIVLSDSEEENEPIIPSGAVFNNNHSDAPVVSFPAQPLGIPDSF-HDSALV 652

Query: 1213 NG----IDLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTT 1046
            NG    + LFN++DD+FGM++WSLPSG+QG PGFQLF SDADV+ +LVDVQH SINC ++
Sbjct: 653  NGGNSCLGLFNSNDDEFGMNLWSLPSGTQGGPGFQLFSSDADVSGSLVDVQHESINCTSS 712

Query: 1045 VNGYTLTAETAMGSAALVPESSVKHTN--VNDGLVDNPLAFSGNDPSLQIFLPTRPSDAS 872
            ++GY L A+T +GSA+L+P +     N  +ND LVDNPL FSGNDPSLQIFLPTRPSD S
Sbjct: 713  MSGYGLAADTGIGSASLLPGTYTDRPNADINDSLVDNPLGFSGNDPSLQIFLPTRPSDTS 772

Query: 871  AQADMRDLPDVSNGIRXVEGVMVSL 797
             +A  RD PDVSNG+   + + + L
Sbjct: 773  VEA-ARDQPDVSNGVGTEDWISLRL 796



 Score = 90.1 bits (222), Expect = 1e-14
 Identities = 48/78 (61%), Positives = 56/78 (71%)
 Frame = -2

Query: 818  GGGHGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXGMNDNKSGKASRERSDSPFSFPR 639
            GG  G+ A +N LSSG Q+Q+K               GMND++S K+SRERSDSPF+FPR
Sbjct: 800  GGVPGDSAVANGLSSGQQVQAKDTALDSLADTASLLIGMNDSRSTKSSRERSDSPFTFPR 859

Query: 638  QRRSVRPRLYLSIDSDSE 585
            QRRSVRPRLYLSIDSDSE
Sbjct: 860  QRRSVRPRLYLSIDSDSE 877


>ref|XP_006340140.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like isoform X1 [Solanum
            tuberosum] gi|565346174|ref|XP_006340141.1| PREDICTED: E3
            SUMO-protein ligase SIZ1-like isoform X2 [Solanum
            tuberosum]
          Length = 885

 Score = 1127 bits (2916), Expect = 0.0
 Identities = 566/805 (70%), Positives = 665/805 (82%), Gaps = 15/805 (1%)
 Frame = -3

Query: 3166 MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDDRVSGMWAKKNAV 2987
            MDLV SCKDKLAYFRIKELKDVL QLGLSKQGKKQDLVDRI+A LSD+R SG++ K+N+V
Sbjct: 1    MDLVASCKDKLAYFRIKELKDVLNQLGLSKQGKKQDLVDRIIATLSDERASGLFPKRNSV 60

Query: 2986 GKEEVAKLVDDTYRKMQISGAT------DLASKSDSSNVKLNVKPKEETEEPYQMDKIRC 2825
            GKE+VAKLVDD YRKMQ+SGAT      DLASKS   +   NVK KEE E+ Y M KIRC
Sbjct: 61   GKEDVAKLVDDIYRKMQVSGATGATGATDLASKSQVVSDTSNVKLKEEIEDTYHM-KIRC 119

Query: 2824 LCGSTLPTDSMIKCEDPRCNVWQHIACVVIPEKPVEGVLPNTPEI-FYCEICRLSRADPF 2648
            +C S+L T++MI+CED RC+ WQHI CVVIP+KP+EG  P  P   FYCE+CRL RADPF
Sbjct: 120  VCTSSLQTETMIQCEDRRCHTWQHIRCVVIPDKPMEGGDPPIPPTTFYCEVCRLVRADPF 179

Query: 2647 WMTVAHPLYPVKLTITSVPSDGSNPVQSIEKTFQLTRADKDLLSKQEYDVQAWCMLLNDK 2468
            W+T+ HPLYP KL ITSVP+DG+NPVQSIEKTFQ+TRAD+DLL+KQEYD+QAWCMLLNDK
Sbjct: 180  WVTMGHPLYPAKLAITSVPADGTNPVQSIEKTFQITRADRDLLAKQEYDLQAWCMLLNDK 239

Query: 2467 VPFRMQWPLYADLQINGVPVRAINRPGSQQLGANGRDDGPNITPCTRDGINKISLTGCDA 2288
            V FRMQWP YADLQ+NGVPVRAINRPGSQ LGANGRDDGP ITPCTRDGINK++LTGCDA
Sbjct: 240  VQFRMQWPQYADLQVNGVPVRAINRPGSQLLGANGRDDGPIITPCTRDGINKVTLTGCDA 299

Query: 2287 RIFCLGVRIVKRRTLHQVLNIIPKESEGERFEDALARVRRCVGGGAATENADSDSDIEVV 2108
            R+FCLGVR+VKRRT+ QVL++IPK S+GE+FEDALARVRRCVGGG ATENADSDSD+EVV
Sbjct: 300  RVFCLGVRLVKRRTVQQVLSMIPKVSDGEKFEDALARVRRCVGGGTATENADSDSDLEVV 359

Query: 2107 ADFIPVNLRCPMSGLRMKVAGRFEPCVHMGCFDLEVFVEMNLRSRKWQCPICLKNYSLEK 1928
            AD IPVNLRCPMSG RMKVAGRF+PC+HMGCFDL+VFVEMN RSRKWQCPICLKNYSLE 
Sbjct: 360  ADCIPVNLRCPMSGSRMKVAGRFKPCIHMGCFDLDVFVEMNQRSRKWQCPICLKNYSLEH 419

Query: 1927 IIIDPYFNRITSKIRNCGEDVNEIEVKPDGSWRAKTEGDRRALGELGLWHLPDGNLCPSM 1748
            +IIDPYFNRITS++R+CGEDV EIEVKPDGSWRAKTE DRR+LG+LG WHLPDG+L  S 
Sbjct: 420  VIIDPYFNRITSQMRSCGEDVTEIEVKPDGSWRAKTESDRRSLGDLGRWHLPDGSLSESP 479

Query: 1747 EGESKPKQEFVKQIKQEGGSDSHAGLKLGMKRNRNGCWELNKSDDILKMSSASRLQEHFE 1568
            + ESKPK E +KQ+KQEGGSD + GLK+G+K+NR+G WE++K +D    SS +RL+E+F 
Sbjct: 480  DIESKPKPEILKQVKQEGGSDGN-GLKVGLKKNRDGLWEISKPED-QTFSSGNRLRENFG 537

Query: 1567 NDDHNVIPMSSSATGSGRDGEDASVNQDGAENLDFATNNGIELESISLNMDQSYGFNEQN 1388
             D   VIPMSSSATGSG++GED SVNQDG  NL+F +NN  +LE+ISLN+D  YGF   N
Sbjct: 538  QD---VIPMSSSATGSGKEGEDRSVNQDGNGNLEF-SNNAFDLEAISLNIDPPYGFGHGN 593

Query: 1387 --LPAPLEEVIILSDSEEENEPLISSGAVYKNNHVDNGGTAFPISQHGIPGSYPEDPAFG 1214
              +PA   EVI+LSDSEEENEP+I SGAV+ NNH D    +FP    GIP S+  D A  
Sbjct: 594  PSVPAGDAEVIVLSDSEEENEPIIPSGAVFNNNHSDAPVVSFPAQPLGIPDSF-HDSALV 652

Query: 1213 NG----IDLFNAHDDDFGMHMWSLPSGSQGAPGFQLFGSDADVTDALVDVQHGSINCPTT 1046
            NG    + LFN++DD+FGM++WSLPSG+QG PGFQLF SDADV+ +LVDVQH SINC ++
Sbjct: 653  NGGNSCLGLFNSNDDEFGMNLWSLPSGTQGGPGFQLFSSDADVSGSLVDVQHESINCTSS 712

Query: 1045 VNGYTLTAETAMGSAALVPESSVKHTN--VNDGLVDNPLAFSGNDPSLQIFLPTRPSDAS 872
            ++GY L A+T +GSA+L+P +     N  +ND LVDNPL FSGNDPSLQIFLPTRPSD S
Sbjct: 713  MSGYGLAADTGIGSASLLPGTYTDRPNADINDSLVDNPLGFSGNDPSLQIFLPTRPSDTS 772

Query: 871  AQADMRDLPDVSNGIRXVEGVMVSL 797
             +A  RD PDVSNG+   + + + L
Sbjct: 773  VEA-ARDQPDVSNGVGTEDWISLRL 796



 Score = 82.8 bits (203), Expect = 2e-12
 Identities = 47/86 (54%), Positives = 55/86 (63%), Gaps = 8/86 (9%)
 Frame = -2

Query: 818  GGGHGEPAASNVLSSGNQLQSKXXXXXXXXXXXXXXXG--------MNDNKSGKASRERS 663
            GG  G+ A +N LSSG Q+Q+K                        MND++S K+SRERS
Sbjct: 800  GGVPGDSAVANGLSSGQQVQAKDTALDSLADTGSAAPVCAASLLIGMNDSRSTKSSRERS 859

Query: 662  DSPFSFPRQRRSVRPRLYLSIDSDSE 585
            DSPF+FPRQRRSVRPRLYLSIDSDSE
Sbjct: 860  DSPFTFPRQRRSVRPRLYLSIDSDSE 885