BLASTX nr result
ID: Forsythia21_contig00020258
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Forsythia21_contig00020258
(312 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AHL44988.1| 4-coumarate:coenzyme A ligase 4 [Fraxinus mandshu... 167 3e-39
gb|KMT00862.1| hypothetical protein BVRB_9g221350 isoform B [Bet... 139 6e-31
ref|XP_010691379.1| PREDICTED: malonate--CoA ligase isoform X2 [... 139 6e-31
ref|XP_011072386.1| PREDICTED: malonate--CoA ligase isoform X2 [... 137 2e-30
ref|XP_011072385.1| PREDICTED: malonate--CoA ligase isoform X1 [... 137 2e-30
gb|KMT00861.1| hypothetical protein BVRB_9g221350 isoform A [Bet... 136 5e-30
ref|XP_010691378.1| PREDICTED: malonate--CoA ligase isoform X1 [... 136 5e-30
ref|XP_012856547.1| PREDICTED: malonate--CoA ligase [Erythranthe... 133 5e-29
ref|XP_010254053.1| PREDICTED: malonate--CoA ligase [Nelumbo nuc... 131 2e-28
ref|XP_010314289.1| PREDICTED: malonate--CoA ligase [Solanum lyc... 127 3e-27
emb|CDO98015.1| unnamed protein product [Coffea canephora] 127 3e-27
gb|AGA17925.1| CCL8 [Humulus lupulus] 127 3e-27
ref|XP_006369195.1| hypothetical protein POPTR_0001s18610g [Popu... 127 4e-27
ref|XP_010924575.1| PREDICTED: malonate--CoA ligase-like [Elaeis... 126 5e-27
ref|XP_010654650.1| PREDICTED: malonate--CoA ligase isoform X2 [... 126 6e-27
ref|XP_010654649.1| PREDICTED: malonate--CoA ligase isoform X1 [... 126 6e-27
ref|XP_009800043.1| PREDICTED: malonate--CoA ligase-like isoform... 125 8e-27
ref|XP_009800042.1| PREDICTED: malonate--CoA ligase-like isoform... 125 8e-27
ref|XP_006359460.1| PREDICTED: malonate--CoA ligase-like [Solanu... 125 8e-27
ref|XP_003567137.1| PREDICTED: LOW QUALITY PROTEIN: malonate--Co... 125 8e-27
>gb|AHL44988.1| 4-coumarate:coenzyme A ligase 4 [Fraxinus mandshurica]
Length = 328
Score = 167 bits (422), Expect = 3e-39
Identities = 85/103 (82%), Positives = 91/103 (88%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSIYDHVDTKNE 131
ALSYPEAELLHVMNDSDV VILSTEDHQ+L+K VA KAAAQ+SLIPPI SI D D NE
Sbjct: 140 ALSYPEAELLHVMNDSDVTVILSTEDHQKLMKVVAAKAAAQVSLIPPIPSINDLADKTNE 199
Query: 130 PIEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
I+ S+KINGNDAALI+YTSGTTGKPKGVVHTHKGV AQVQML
Sbjct: 200 EIKISDKINGNDAALILYTSGTTGKPKGVVHTHKGVFAQVQML 242
>gb|KMT00862.1| hypothetical protein BVRB_9g221350 isoform B [Beta vulgaris subsp.
vulgaris]
Length = 566
Score = 139 bits (351), Expect = 6e-31
Identities = 73/116 (62%), Positives = 87/116 (75%), Gaps = 13/116 (11%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSI--------- 158
ALSYPEAELLHVMNDSDV+++LSTEDHQEL++ VA K+AAQ SLIP I S+
Sbjct: 106 ALSYPEAELLHVMNDSDVSIVLSTEDHQELMETVASKSAAQFSLIPSIPSMTSETNPNGH 165
Query: 157 ----YDHVDTKNEPIEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
Y + +T + E KI G++AAL+IYTSGTTGKPKGVVHTH+ VLAQVQML
Sbjct: 166 SESEYPNENTSLKEFEIPTKIEGDNAALVIYTSGTTGKPKGVVHTHRSVLAQVQML 221
>ref|XP_010691379.1| PREDICTED: malonate--CoA ligase isoform X2 [Beta vulgaris subsp.
vulgaris]
Length = 618
Score = 139 bits (351), Expect = 6e-31
Identities = 73/116 (62%), Positives = 87/116 (75%), Gaps = 13/116 (11%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSI--------- 158
ALSYPEAELLHVMNDSDV+++LSTEDHQEL++ VA K+AAQ SLIP I S+
Sbjct: 158 ALSYPEAELLHVMNDSDVSIVLSTEDHQELMETVASKSAAQFSLIPSIPSMTSETNPNGH 217
Query: 157 ----YDHVDTKNEPIEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
Y + +T + E KI G++AAL+IYTSGTTGKPKGVVHTH+ VLAQVQML
Sbjct: 218 SESEYPNENTSLKEFEIPTKIEGDNAALVIYTSGTTGKPKGVVHTHRSVLAQVQML 273
>ref|XP_011072386.1| PREDICTED: malonate--CoA ligase isoform X2 [Sesamum indicum]
Length = 552
Score = 137 bits (346), Expect = 2e-30
Identities = 73/116 (62%), Positives = 87/116 (75%), Gaps = 13/116 (11%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSI------YDH 149
ALSYPEAELLHVMNDSD+ +ILSTEDHQEL+KA+A K +AQ+SL+P + S+ DH
Sbjct: 93 ALSYPEAELLHVMNDSDITMILSTEDHQELMKAIAVKTSAQMSLLPSVPSVSTKSTELDH 152
Query: 148 VDTKN-------EPIEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
+ E ++I+G+D ALIIYTSGTTGKPKGVVHTHKGVLAQVQML
Sbjct: 153 TINGELDGTRCLQETEIPHEISGDDPALIIYTSGTTGKPKGVVHTHKGVLAQVQML 208
>ref|XP_011072385.1| PREDICTED: malonate--CoA ligase isoform X1 [Sesamum indicum]
Length = 620
Score = 137 bits (346), Expect = 2e-30
Identities = 73/116 (62%), Positives = 87/116 (75%), Gaps = 13/116 (11%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSI------YDH 149
ALSYPEAELLHVMNDSD+ +ILSTEDHQEL+KA+A K +AQ+SL+P + S+ DH
Sbjct: 161 ALSYPEAELLHVMNDSDITMILSTEDHQELMKAIAVKTSAQMSLLPSVPSVSTKSTELDH 220
Query: 148 VDTKN-------EPIEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
+ E ++I+G+D ALIIYTSGTTGKPKGVVHTHKGVLAQVQML
Sbjct: 221 TINGELDGTRCLQETEIPHEISGDDPALIIYTSGTTGKPKGVVHTHKGVLAQVQML 276
>gb|KMT00861.1| hypothetical protein BVRB_9g221350 isoform A [Beta vulgaris subsp.
vulgaris]
Length = 567
Score = 136 bits (343), Expect = 5e-30
Identities = 74/117 (63%), Positives = 86/117 (73%), Gaps = 14/117 (11%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSI--------- 158
ALSYPEAELLHVMNDSDV+++LSTEDHQEL++ VA K+AAQ SLIP I S+
Sbjct: 106 ALSYPEAELLHVMNDSDVSIVLSTEDHQELMETVASKSAAQFSLIPSIPSMTSETNPNGH 165
Query: 157 ----YDHVDTKNEPIEFSNKINGND-AALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
Y + +T + E KI G D AAL+IYTSGTTGKPKGVVHTH+ VLAQVQML
Sbjct: 166 SESEYPNENTSLKEFEIPTKIEGGDNAALVIYTSGTTGKPKGVVHTHRSVLAQVQML 222
>ref|XP_010691378.1| PREDICTED: malonate--CoA ligase isoform X1 [Beta vulgaris subsp.
vulgaris]
Length = 619
Score = 136 bits (343), Expect = 5e-30
Identities = 74/117 (63%), Positives = 86/117 (73%), Gaps = 14/117 (11%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSI--------- 158
ALSYPEAELLHVMNDSDV+++LSTEDHQEL++ VA K+AAQ SLIP I S+
Sbjct: 158 ALSYPEAELLHVMNDSDVSIVLSTEDHQELMETVASKSAAQFSLIPSIPSMTSETNPNGH 217
Query: 157 ----YDHVDTKNEPIEFSNKINGND-AALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
Y + +T + E KI G D AAL+IYTSGTTGKPKGVVHTH+ VLAQVQML
Sbjct: 218 SESEYPNENTSLKEFEIPTKIEGGDNAALVIYTSGTTGKPKGVVHTHRSVLAQVQML 274
>ref|XP_012856547.1| PREDICTED: malonate--CoA ligase [Erythranthe guttatus]
gi|604301889|gb|EYU21475.1| hypothetical protein
MIMGU_mgv1a003128mg [Erythranthe guttata]
Length = 606
Score = 133 bits (334), Expect = 5e-29
Identities = 67/107 (62%), Positives = 83/107 (77%), Gaps = 4/107 (3%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSIYDHVD---- 143
ALSYPEAELLHVMNDSD+ +ILST+DHQ +++ VA K A+LSL+P + ++
Sbjct: 155 ALSYPEAELLHVMNDSDITMILSTDDHQGIMEGVAAKTCARLSLLPHVPNVITESTELDC 214
Query: 142 TKNEPIEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
+ N + S++ING D ALIIYTSGTTGKPKGVVHTHKGVL+QVQML
Sbjct: 215 SSNGEFKISHEINGEDPALIIYTSGTTGKPKGVVHTHKGVLSQVQML 261
>ref|XP_010254053.1| PREDICTED: malonate--CoA ligase [Nelumbo nucifera]
Length = 615
Score = 131 bits (330), Expect = 2e-28
Identities = 68/107 (63%), Positives = 82/107 (76%), Gaps = 4/107 (3%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSIYDHVDTKN- 134
ALSYPEAEL HVM DSDV+++LSTED+QEL++ VA K AAQ SLIPP+ S Y +T+
Sbjct: 166 ALSYPEAELFHVMKDSDVSMLLSTEDYQELMENVASKCAAQFSLIPPVPSGYSIEETEEK 225
Query: 133 ---EPIEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
+ IE S + G + ALI+YTSGTTGKPKGVVHTHK + AQVQML
Sbjct: 226 DLLKEIENSKIVEGENPALIVYTSGTTGKPKGVVHTHKSIAAQVQML 272
>ref|XP_010314289.1| PREDICTED: malonate--CoA ligase [Solanum lycopersicum]
Length = 601
Score = 127 bits (319), Expect = 3e-27
Identities = 67/113 (59%), Positives = 82/113 (72%), Gaps = 10/113 (8%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSIYDHV----- 146
ALSYPE+ELLHVMNDSD+++ILSTEDHQEL+ A+A K AQLSLIP I ++
Sbjct: 145 ALSYPESELLHVMNDSDISIILSTEDHQELMNAIAAKTGAQLSLIPSIPDVHSSTKHDQS 204
Query: 145 -----DTKNEPIEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
D + + +E ++ G + A I+YTSGTTGKPKGVVHTH GVLAQVQML
Sbjct: 205 KDMVSDGQQDLLEINS--YGENPAFILYTSGTTGKPKGVVHTHNGVLAQVQML 255
>emb|CDO98015.1| unnamed protein product [Coffea canephora]
Length = 303
Score = 127 bits (319), Expect = 3e-27
Identities = 68/113 (60%), Positives = 80/113 (70%), Gaps = 10/113 (8%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSIYDHVDTKN- 134
ALSYPEAELLHVMNDSD+++ILSTEDHQELLK VA K AA SL+PP+ +D +
Sbjct: 162 ALSYPEAELLHVMNDSDISMILSTEDHQELLKDVAAKTAAHFSLLPPVIGTSTELDQTHN 221
Query: 133 ---------EPIEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
+ IE + I ALI+YTSGTTGKPKGVVHTH+ +LAQVQML
Sbjct: 222 GELNVIERVQGIENFSAIEDEKPALILYTSGTTGKPKGVVHTHRSILAQVQML 274
>gb|AGA17925.1| CCL8 [Humulus lupulus]
Length = 607
Score = 127 bits (319), Expect = 3e-27
Identities = 67/112 (59%), Positives = 82/112 (73%), Gaps = 9/112 (8%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISS------IYDH 149
ALSYPEAELLHVM DSD+++ILSTEDHQEL++ +A K AAQ SLIPP+ S DH
Sbjct: 149 ALSYPEAELLHVMTDSDISMILSTEDHQELMQKIAAKTAAQFSLIPPVPSSCSQEGAVDH 208
Query: 148 V---DTKNEPIEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
+ D + I + +I+ + ALI+YTSGTTGKPKGVVHTHK + AQVQ L
Sbjct: 209 LQTGDINTDSILHNTEISNENPALIVYTSGTTGKPKGVVHTHKSINAQVQTL 260
>ref|XP_006369195.1| hypothetical protein POPTR_0001s18610g [Populus trichocarpa]
gi|550347624|gb|ERP65764.1| hypothetical protein
POPTR_0001s18610g [Populus trichocarpa]
Length = 297
Score = 127 bits (318), Expect = 4e-27
Identities = 65/109 (59%), Positives = 80/109 (73%), Gaps = 6/109 (5%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPI------SSIYDH 149
ALSYPE ELLHVMND++++++LSTEDHQEL++ VADK AAQ SLIP + S++DH
Sbjct: 159 ALSYPETELLHVMNDAEISMVLSTEDHQELMQNVADKCAAQFSLIPSVHRISSHESVHDH 218
Query: 148 VDTKNEPIEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
+ +E G+D ALIIYTSGTTGKPKG VHTHK + AQVQ L
Sbjct: 219 SQVEEIALE-----KGDDPALIIYTSGTTGKPKGAVHTHKSITAQVQTL 262
>ref|XP_010924575.1| PREDICTED: malonate--CoA ligase-like [Elaeis guineensis]
Length = 595
Score = 126 bits (317), Expect = 5e-27
Identities = 65/110 (59%), Positives = 83/110 (75%), Gaps = 7/110 (6%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSIYDHVDTKNE 131
ALSYPEAELLHVMNDSD+++ILST++HQ+LL+ VA K +A LSLIP ++S+ T +
Sbjct: 143 ALSYPEAELLHVMNDSDISMILSTQEHQQLLENVASKCSAHLSLIPAVTSMEQSGSTADN 202
Query: 130 -------PIEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
IE S G+D ALI+YTSGTTGKPKGVVHTHK +++QVQ+L
Sbjct: 203 LVAKLIGEIEDSRVAEGDDPALILYTSGTTGKPKGVVHTHKSIISQVQIL 252
>ref|XP_010654650.1| PREDICTED: malonate--CoA ligase isoform X2 [Vitis vinifera]
Length = 608
Score = 126 bits (316), Expect = 6e-27
Identities = 68/117 (58%), Positives = 83/117 (70%), Gaps = 14/117 (11%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSIYDHVDTKNE 131
ALSYPEAELLHVMNDSDV++ILSTED++EL++ VA K++AQ SLIPP+ SI ++
Sbjct: 147 ALSYPEAELLHVMNDSDVSMILSTEDYRELMENVAAKSSAQFSLIPPVPSIPSPTSARDH 206
Query: 130 P--------------IEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
P I NK+ D ALIIYTSGTTGKPKGVVHTHK + +QVQ+L
Sbjct: 207 PQTGEIVADKSLQGEIGKWNKLEDEDPALIIYTSGTTGKPKGVVHTHKSINSQVQIL 263
>ref|XP_010654649.1| PREDICTED: malonate--CoA ligase isoform X1 [Vitis vinifera]
Length = 609
Score = 126 bits (316), Expect = 6e-27
Identities = 68/117 (58%), Positives = 83/117 (70%), Gaps = 14/117 (11%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSIYDHVDTKNE 131
ALSYPEAELLHVMNDSDV++ILSTED++EL++ VA K++AQ SLIPP+ SI ++
Sbjct: 148 ALSYPEAELLHVMNDSDVSMILSTEDYRELMENVAAKSSAQFSLIPPVPSIPSPTSARDH 207
Query: 130 P--------------IEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
P I NK+ D ALIIYTSGTTGKPKGVVHTHK + +QVQ+L
Sbjct: 208 PQTGEIVADKSLQGEIGKWNKLEDEDPALIIYTSGTTGKPKGVVHTHKSINSQVQIL 264
>ref|XP_009800043.1| PREDICTED: malonate--CoA ligase-like isoform X2 [Nicotiana
sylvestris]
Length = 549
Score = 125 bits (315), Expect = 8e-27
Identities = 69/115 (60%), Positives = 82/115 (71%), Gaps = 12/115 (10%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSIYDHVDTKNE 131
ALSYPE ELLHVMNDSD++VILSTEDHQEL+ +A K A LSLIPPI + TK++
Sbjct: 93 ALSYPETELLHVMNDSDISVILSTEDHQELMNTIAVKTGACLSLIPPIPD--GDLSTKHD 150
Query: 130 P------------IEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
P E +N G D A+I+YTSGTTGKPKGVVHTHKG+LAQ+Q+L
Sbjct: 151 PPKDVVSDGPRNLPEMTN--CGEDPAMILYTSGTTGKPKGVVHTHKGILAQIQIL 203
>ref|XP_009800042.1| PREDICTED: malonate--CoA ligase-like isoform X1 [Nicotiana
sylvestris]
Length = 608
Score = 125 bits (315), Expect = 8e-27
Identities = 69/115 (60%), Positives = 82/115 (71%), Gaps = 12/115 (10%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSIYDHVDTKNE 131
ALSYPE ELLHVMNDSD++VILSTEDHQEL+ +A K A LSLIPPI + TK++
Sbjct: 152 ALSYPETELLHVMNDSDISVILSTEDHQELMNTIAVKTGACLSLIPPIPD--GDLSTKHD 209
Query: 130 P------------IEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
P E +N G D A+I+YTSGTTGKPKGVVHTHKG+LAQ+Q+L
Sbjct: 210 PPKDVVSDGPRNLPEMTN--CGEDPAMILYTSGTTGKPKGVVHTHKGILAQIQIL 262
>ref|XP_006359460.1| PREDICTED: malonate--CoA ligase-like [Solanum tuberosum]
Length = 596
Score = 125 bits (315), Expect = 8e-27
Identities = 66/113 (58%), Positives = 81/113 (71%), Gaps = 10/113 (8%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSIYDHV----- 146
ALSYPE+ELLHVMNDSD+++ILSTEDHQEL+ +A K AQLSLIP I ++
Sbjct: 140 ALSYPESELLHVMNDSDISIILSTEDHQELMNTIAAKTGAQLSLIPSIPDVHSSTKHDQS 199
Query: 145 -----DTKNEPIEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
D + + +E ++ G + A I+YTSGTTGKPKGVVHTH GVLAQVQML
Sbjct: 200 KDMVSDGQQDLLEINS--YGENPAFILYTSGTTGKPKGVVHTHNGVLAQVQML 250
>ref|XP_003567137.1| PREDICTED: LOW QUALITY PROTEIN: malonate--CoA ligase [Brachypodium
distachyon]
Length = 590
Score = 125 bits (315), Expect = 8e-27
Identities = 64/118 (54%), Positives = 88/118 (74%), Gaps = 15/118 (12%)
Frame = -3
Query: 310 ALSYPEAELLHVMNDSDVAVILSTEDHQELLKAVADKAAAQLSLIPPISSIYDHVDTKNE 131
ALSYPE ELLHVMNDSD+++ILST++H +++++++ K +A+ SL+P + SI +D + E
Sbjct: 165 ALSYPEVELLHVMNDSDISMILSTKEHHDIMESISTKCSARYSLLPDVESIPPKMDFQ-E 223
Query: 130 P---------------IEFSNKINGNDAALIIYTSGTTGKPKGVVHTHKGVLAQVQML 2
P I+ SNKI G+D ALI+YTSGTTGKPKGVVHTHKG+L+QVQ+L
Sbjct: 224 PLSSEVASSVYSLMADIDRSNKIKGDDPALILYTSGTTGKPKGVVHTHKGILSQVQIL 281