BLASTX nr result
ID: Forsythia21_contig00016504
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Forsythia21_contig00016504
(294 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_011098224.1| PREDICTED: phosphoenolpyruvate carboxylase [... 182 6e-44
ref|XP_006360045.1| PREDICTED: phosphoenolpyruvate carboxylase-l... 179 8e-43
ref|XP_004248242.1| PREDICTED: phosphoenolpyruvate carboxylase [... 179 8e-43
ref|XP_009607156.1| PREDICTED: phosphoenolpyruvate carboxylase-l... 176 5e-42
dbj|BAB89367.1| phosphoenolpyruvate carboxylase [Nicotiana sylve... 175 1e-41
ref|XP_009762944.1| PREDICTED: phosphoenolpyruvate carboxylase-l... 175 1e-41
ref|XP_009783765.1| PREDICTED: phosphoenolpyruvate carboxylase [... 173 3e-41
ref|XP_009604678.1| PREDICTED: phosphoenolpyruvate carboxylase [... 173 3e-41
dbj|BAB89368.2| phosphoenolpyruvate carboxylase [Nicotiana sylve... 173 3e-41
sp|P27154.1|CAPP_TOBAC RecName: Full=Phosphoenolpyruvate carboxy... 173 3e-41
gb|AIF35296.1| phosphoenolpyruvate carboxylase, partial [Phytola... 170 3e-40
ref|XP_011082759.1| PREDICTED: phosphoenolpyruvate carboxylase-l... 170 4e-40
ref|XP_009766373.1| PREDICTED: phosphoenolpyruvate carboxylase [... 170 4e-40
ref|XP_009598007.1| PREDICTED: phosphoenolpyruvate carboxylase [... 170 4e-40
dbj|BAB89366.1| phosphoenolpyruvate carboxylase [Nicotiana sylve... 170 4e-40
emb|CDP06006.1| unnamed protein product [Coffea canephora] 169 7e-40
ref|NP_001267517.1| phosphoenolpyruvate carboxylase, housekeepin... 169 7e-40
emb|CAD10147.1| phosphoenolpyruvate carboxylase [Cucumis sativus] 169 7e-40
ref|XP_012848136.1| PREDICTED: phosphoenolpyruvate carboxylase [... 168 1e-39
emb|CBM40378.1| phosphoenolpyruvate carboxylase, partial [Mollug... 168 1e-39
>ref|XP_011098224.1| PREDICTED: phosphoenolpyruvate carboxylase [Sesamum indicum]
Length = 964
Score = 182 bits (463), Expect = 6e-44
Identities = 89/97 (91%), Positives = 94/97 (96%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDLWSFG+ +RANYEETKSLLLQIAGHK++LEGDPYLKQRLRLRDSYITTL
Sbjct: 836 ALYDKLLVSEDLWSFGERLRANYEETKSLLLQIAGHKDLLEGDPYLKQRLRLRDSYITTL 895
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAEL 2
NVSQAYTLKRIRDPNYHVKLRPHISK+YMES AAEL
Sbjct: 896 NVSQAYTLKRIRDPNYHVKLRPHISKEYMESKPAAEL 932
>ref|XP_006360045.1| PREDICTED: phosphoenolpyruvate carboxylase-like isoform X1 [Solanum
tuberosum] gi|565388567|ref|XP_006360046.1| PREDICTED:
phosphoenolpyruvate carboxylase-like isoform X2 [Solanum
tuberosum]
Length = 964
Score = 179 bits (453), Expect = 8e-43
Identities = 86/97 (88%), Positives = 94/97 (96%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDLWSFG+L+R+ YEET++LLLQIAGHK++LEGDPYLKQRLRLRDSYITTL
Sbjct: 836 ALYDKLLVSEDLWSFGELLRSKYEETRTLLLQIAGHKDLLEGDPYLKQRLRLRDSYITTL 895
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAEL 2
NV QAYTLKRIRDPNYHVKLRPHISK+YMES SAAEL
Sbjct: 896 NVLQAYTLKRIRDPNYHVKLRPHISKEYMESKSAAEL 932
>ref|XP_004248242.1| PREDICTED: phosphoenolpyruvate carboxylase [Solanum lycopersicum]
Length = 964
Score = 179 bits (453), Expect = 8e-43
Identities = 86/97 (88%), Positives = 94/97 (96%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDLWSFG+L+R+ YEET++LLLQIAGHK++LEGDPYLKQRLRLRDSYITTL
Sbjct: 836 ALYDKLLVSEDLWSFGELLRSKYEETRTLLLQIAGHKDLLEGDPYLKQRLRLRDSYITTL 895
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAEL 2
NV QAYTLKRIRDPNYHVKLRPHISK+YMES SAAEL
Sbjct: 896 NVLQAYTLKRIRDPNYHVKLRPHISKEYMESKSAAEL 932
>ref|XP_009607156.1| PREDICTED: phosphoenolpyruvate carboxylase-like [Nicotiana
tomentosiformis] gi|697106674|ref|XP_009607157.1|
PREDICTED: phosphoenolpyruvate carboxylase-like
[Nicotiana tomentosiformis]
Length = 964
Score = 176 bits (446), Expect = 5e-42
Identities = 85/97 (87%), Positives = 92/97 (94%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDLWSFG+L+RANYEETKSLLLQIAGHK++LEGDPYLKQRLRLRDSYITTL
Sbjct: 836 ALYDKLLVSEDLWSFGELLRANYEETKSLLLQIAGHKDLLEGDPYLKQRLRLRDSYITTL 895
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAEL 2
NV QAYTLKR+RDPNY V LRPHI+K+YMES AAEL
Sbjct: 896 NVCQAYTLKRVRDPNYRVTLRPHITKEYMESKPAAEL 932
>dbj|BAB89367.1| phosphoenolpyruvate carboxylase [Nicotiana sylvestris]
Length = 820
Score = 175 bits (443), Expect = 1e-41
Identities = 85/97 (87%), Positives = 92/97 (94%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDLWSFG+L+RANYEETKSLLLQIAGHK++LEGDPYLKQRLRLRDSYITTL
Sbjct: 693 ALYDKLLVSEDLWSFGELLRANYEETKSLLLQIAGHKDLLEGDPYLKQRLRLRDSYITTL 752
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAEL 2
NV QAYTLKR+RDPNY V LRPHI+K+YMES AAEL
Sbjct: 753 NVCQAYTLKRVRDPNYLVTLRPHITKEYMESKPAAEL 789
>ref|XP_009762944.1| PREDICTED: phosphoenolpyruvate carboxylase-like [Nicotiana
sylvestris]
Length = 964
Score = 175 bits (443), Expect = 1e-41
Identities = 85/97 (87%), Positives = 92/97 (94%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDLWSFG+L+RANYEETKSLLLQIAGHK++LEGDPYLKQRLRLRDSYITTL
Sbjct: 836 ALYDKLLVSEDLWSFGELLRANYEETKSLLLQIAGHKDLLEGDPYLKQRLRLRDSYITTL 895
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAEL 2
NV QAYTLKR+RDPNY V LRPHI+K+YMES AAEL
Sbjct: 896 NVCQAYTLKRVRDPNYLVTLRPHITKEYMESKPAAEL 932
>ref|XP_009783765.1| PREDICTED: phosphoenolpyruvate carboxylase [Nicotiana sylvestris]
gi|698470241|ref|XP_009783766.1| PREDICTED:
phosphoenolpyruvate carboxylase [Nicotiana sylvestris]
Length = 964
Score = 173 bits (439), Expect = 3e-41
Identities = 85/97 (87%), Positives = 92/97 (94%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDL FG+L+R+NYEET+SLLLQIAGHK++LEGDPYLKQRLRLRDSYITTL
Sbjct: 836 ALYDKLLVSEDLLPFGELLRSNYEETRSLLLQIAGHKDLLEGDPYLKQRLRLRDSYITTL 895
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAEL 2
N+ QAYTLKRIRDPNYHV LRPHISKDYMES SAAEL
Sbjct: 896 NLLQAYTLKRIRDPNYHVTLRPHISKDYMESKSAAEL 932
>ref|XP_009604678.1| PREDICTED: phosphoenolpyruvate carboxylase [Nicotiana
tomentosiformis] gi|697191222|ref|XP_009604679.1|
PREDICTED: phosphoenolpyruvate carboxylase [Nicotiana
tomentosiformis] gi|697191224|ref|XP_009604680.1|
PREDICTED: phosphoenolpyruvate carboxylase [Nicotiana
tomentosiformis]
Length = 964
Score = 173 bits (439), Expect = 3e-41
Identities = 85/97 (87%), Positives = 92/97 (94%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDL FG+L+R+NYEET+SLLLQIAGHK++LEGDPYLKQRLRLRDSYITTL
Sbjct: 836 ALYDKLLVSEDLLPFGELLRSNYEETRSLLLQIAGHKDLLEGDPYLKQRLRLRDSYITTL 895
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAEL 2
N+ QAYTLKRIRDPNYHV LRPHISKDYMES SAAEL
Sbjct: 896 NLLQAYTLKRIRDPNYHVTLRPHISKDYMESKSAAEL 932
>dbj|BAB89368.2| phosphoenolpyruvate carboxylase [Nicotiana sylvestris]
Length = 657
Score = 173 bits (439), Expect = 3e-41
Identities = 85/97 (87%), Positives = 92/97 (94%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDL FG+L+R+NYEET+SLLLQIAGHK++LEGDPYLKQRLRLRDSYITTL
Sbjct: 529 ALYDKLLVSEDLLPFGELLRSNYEETRSLLLQIAGHKDLLEGDPYLKQRLRLRDSYITTL 588
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAEL 2
N+ QAYTLKRIRDPNYHV LRPHISKDYMES SAAEL
Sbjct: 589 NLLQAYTLKRIRDPNYHVTLRPHISKDYMESKSAAEL 625
>sp|P27154.1|CAPP_TOBAC RecName: Full=Phosphoenolpyruvate carboxylase; Short=PEPC;
Short=PEPCase [Nicotiana tabacum]
gi|22589|emb|CAA41758.1| phosphoenolpyruvate carboxylase
[Nicotiana tabacum]
Length = 964
Score = 173 bits (439), Expect = 3e-41
Identities = 85/97 (87%), Positives = 92/97 (94%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDL FG+L+R+NYEET+SLLLQIAGHK++LEGDPYLKQRLRLRDSYITTL
Sbjct: 836 ALYDKLLVSEDLLPFGELLRSNYEETRSLLLQIAGHKDLLEGDPYLKQRLRLRDSYITTL 895
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAEL 2
N+ QAYTLKRIRDPNYHV LRPHISKDYMES SAAEL
Sbjct: 896 NLLQAYTLKRIRDPNYHVTLRPHISKDYMESKSAAEL 932
>gb|AIF35296.1| phosphoenolpyruvate carboxylase, partial [Phytolacca dioica]
Length = 527
Score = 170 bits (431), Expect = 3e-40
Identities = 84/99 (84%), Positives = 94/99 (94%), Gaps = 2/99 (2%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSE+LWSFGQ +R N+EETK+LLL+IAGHK++LEGDPYL+QRLRLRDSYITTL
Sbjct: 404 ALYDKLLVSEELWSFGQRLRTNFEETKNLLLKIAGHKDLLEGDPYLRQRLRLRDSYITTL 463
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSS--AAEL 2
NV QAYTLKRIRDPNYHVKLRPHISK+YMES+S AAEL
Sbjct: 464 NVLQAYTLKRIRDPNYHVKLRPHISKEYMESASKPAAEL 502
>ref|XP_011082759.1| PREDICTED: phosphoenolpyruvate carboxylase-like [Sesamum indicum]
Length = 964
Score = 170 bits (430), Expect = 4e-40
Identities = 83/97 (85%), Positives = 91/97 (93%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDLWSFG+ +R+NYEETK+LLLQIA HK++LEGD +LKQRLRLRDSYITTL
Sbjct: 836 ALYDKLLVSEDLWSFGERLRSNYEETKTLLLQIADHKDLLEGDLHLKQRLRLRDSYITTL 895
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAEL 2
NV QAYTLKRIRDP YHVKLRPHISKDYMES +AAEL
Sbjct: 896 NVCQAYTLKRIRDPTYHVKLRPHISKDYMESKAAAEL 932
>ref|XP_009766373.1| PREDICTED: phosphoenolpyruvate carboxylase [Nicotiana sylvestris]
Length = 966
Score = 170 bits (430), Expect = 4e-40
Identities = 83/97 (85%), Positives = 90/97 (92%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDLWSFG+ +R+NYEETKSLLLQIAGHK++LEGDPYL+QRLRLRDSYITTL
Sbjct: 838 ALYDKLLVSEDLWSFGEQLRSNYEETKSLLLQIAGHKDLLEGDPYLRQRLRLRDSYITTL 897
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAEL 2
NV QAYTLKRIRDPNY V RPHISK+YMES AAEL
Sbjct: 898 NVCQAYTLKRIRDPNYSVTPRPHISKEYMESKPAAEL 934
>ref|XP_009598007.1| PREDICTED: phosphoenolpyruvate carboxylase [Nicotiana
tomentosiformis]
Length = 966
Score = 170 bits (430), Expect = 4e-40
Identities = 83/97 (85%), Positives = 90/97 (92%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDLWSFG+ +R+NYEETKSLLLQIAGHK++LEGDPYL+QRLRLRDSYITTL
Sbjct: 838 ALYDKLLVSEDLWSFGEQLRSNYEETKSLLLQIAGHKDLLEGDPYLRQRLRLRDSYITTL 897
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAEL 2
NV QAYTLKRIRDPNY V RPHISK+YMES AAEL
Sbjct: 898 NVCQAYTLKRIRDPNYSVTPRPHISKEYMESKPAAEL 934
>dbj|BAB89366.1| phosphoenolpyruvate carboxylase [Nicotiana sylvestris]
Length = 750
Score = 170 bits (430), Expect = 4e-40
Identities = 83/97 (85%), Positives = 90/97 (92%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDLWSFG+ +R+NYEETKSLLLQIAGHK++LEGDPYL+QRLRLRDSYITTL
Sbjct: 622 ALYDKLLVSEDLWSFGEQLRSNYEETKSLLLQIAGHKDLLEGDPYLRQRLRLRDSYITTL 681
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAEL 2
NV QAYTLKRIRDPNY V RPHISK+YMES AAEL
Sbjct: 682 NVCQAYTLKRIRDPNYSVTPRPHISKEYMESKPAAEL 718
>emb|CDP06006.1| unnamed protein product [Coffea canephora]
Length = 965
Score = 169 bits (428), Expect = 7e-40
Identities = 80/96 (83%), Positives = 91/96 (94%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDLW+FG+ +R NYE+TK+LLLQIAGHK++LEGDPYLKQ+LRLRDSYITTL
Sbjct: 837 ALYDKLLVSEDLWAFGERLRTNYEQTKTLLLQIAGHKDLLEGDPYLKQQLRLRDSYITTL 896
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAE 5
N+ QAYTLKRIRDPNYHV+LRPHISK+YMES AAE
Sbjct: 897 NLLQAYTLKRIRDPNYHVELRPHISKEYMESKLAAE 932
>ref|NP_001267517.1| phosphoenolpyruvate carboxylase, housekeeping isozyme-like [Cucumis
sativus] gi|778689083|ref|XP_011652896.1| PREDICTED:
phosphoenolpyruvate carboxylase, housekeeping
isozyme-like isoform X1 [Cucumis sativus]
gi|406353249|gb|AFS33790.1| phosphoenolpyruvate
carboxylase protein [Cucumis sativus]
gi|700199913|gb|KGN55071.1| hypothetical protein
Csa_4G627210 [Cucumis sativus]
Length = 965
Score = 169 bits (428), Expect = 7e-40
Identities = 81/96 (84%), Positives = 90/96 (93%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDLWSFG+ +RANYEETKSLLL+IAGH ++LEGDPYLKQRLRLRDSYITTL
Sbjct: 836 ALYDKLLVSEDLWSFGERLRANYEETKSLLLKIAGHSDLLEGDPYLKQRLRLRDSYITTL 895
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAE 5
NV QAYTLKRIRDPNYHVK+RPHISK+ ME+S A+
Sbjct: 896 NVCQAYTLKRIRDPNYHVKVRPHISKEIMEASKPAD 931
>emb|CAD10147.1| phosphoenolpyruvate carboxylase [Cucumis sativus]
Length = 198
Score = 169 bits (428), Expect = 7e-40
Identities = 81/96 (84%), Positives = 90/96 (93%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDLWSFG+ +RANYEETKSLLL+IAGH ++LEGDPYLKQRLRLRDSYITTL
Sbjct: 69 ALYDKLLVSEDLWSFGERLRANYEETKSLLLKIAGHSDLLEGDPYLKQRLRLRDSYITTL 128
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSAAE 5
NV QAYTLKRIRDPNYHVK+RPHISK+ ME+S A+
Sbjct: 129 NVCQAYTLKRIRDPNYHVKVRPHISKEIMEASKPAD 164
>ref|XP_012848136.1| PREDICTED: phosphoenolpyruvate carboxylase [Erythranthe guttatus]
gi|604346576|gb|EYU45020.1| hypothetical protein
MIMGU_mgv1a000847mg [Erythranthe guttata]
Length = 963
Score = 168 bits (426), Expect = 1e-39
Identities = 80/94 (85%), Positives = 89/94 (94%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDLWSFG+ +R+NYEETK+LLLQIAGHK++LEGDP+LKQRLRLRDSYITTL
Sbjct: 836 ALYDKLLVSEDLWSFGERLRSNYEETKTLLLQIAGHKDLLEGDPHLKQRLRLRDSYITTL 895
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSSA 11
N QAYTLKRIRDPNYHVKLRPHIS +YMES +A
Sbjct: 896 NACQAYTLKRIRDPNYHVKLRPHISMEYMESKAA 929
>emb|CBM40378.1| phosphoenolpyruvate carboxylase, partial [Mollugo nudicaulis]
Length = 522
Score = 168 bits (426), Expect = 1e-39
Identities = 83/99 (83%), Positives = 92/99 (92%), Gaps = 2/99 (2%)
Frame = -3
Query: 292 ALYDKLLVSEDLWSFGQLVRANYEETKSLLLQIAGHKEILEGDPYLKQRLRLRDSYITTL 113
ALYDKLLVSEDLWSFG+ +R NYEETK LLL+IA HK++LEGDPYL+QRL+LRDSYITTL
Sbjct: 399 ALYDKLLVSEDLWSFGERLRKNYEETKDLLLKIAAHKDLLEGDPYLRQRLKLRDSYITTL 458
Query: 112 NVSQAYTLKRIRDPNYHVKLRPHISKDYMESSS--AAEL 2
NV QAYTLKRIRDPNYHVKLRPHISK+YMES+S AAEL
Sbjct: 459 NVCQAYTLKRIRDPNYHVKLRPHISKEYMESASKPAAEL 497