BLASTX nr result
ID: Forsythia21_contig00012800
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Forsythia21_contig00012800
(1415 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_011070448.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-r... 320 1e-84
emb|CDP14406.1| unnamed protein product [Coffea canephora] 308 9e-81
ref|XP_009622651.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-r... 303 2e-79
ref|XP_012839641.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-r... 302 5e-79
ref|XP_009775151.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-r... 301 1e-78
ref|XP_004231468.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-r... 298 9e-78
ref|XP_006365590.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-r... 295 4e-77
ref|XP_006382946.1| hypothetical protein POPTR_0005s09300g [Popu... 289 4e-75
ref|XP_010655908.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-r... 285 8e-74
ref|XP_006382945.1| hypothetical protein POPTR_0005s09300g [Popu... 285 8e-74
ref|XP_010655907.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-r... 284 1e-73
ref|XP_009366271.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-r... 282 4e-73
ref|XP_010261280.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-r... 282 5e-73
ref|XP_008383690.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-r... 281 1e-72
ref|XP_007043263.1| RING/U-box superfamily protein [Theobroma ca... 281 1e-72
ref|XP_004497226.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-r... 281 1e-72
ref|XP_004287200.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-r... 280 1e-72
ref|XP_008243095.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-r... 280 3e-72
ref|XP_007202272.1| hypothetical protein PRUPE_ppa008876mg [Prun... 280 3e-72
ref|XP_004497227.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-r... 279 3e-72
>ref|XP_011070448.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-related [Sesamum indicum]
Length = 303
Score = 320 bits (821), Expect = 1e-84
Identities = 190/318 (59%), Positives = 205/318 (64%), Gaps = 11/318 (3%)
Frame = -2
Query: 1219 MENTNSKANTNNTCEQE-----DLTQNPRPGIXXXXXXXXXXXXXXXNDDAEDNRRQRTP 1055
MEN+N+KA+ +NT D QNP D NRRQRTP
Sbjct: 1 MENSNAKADADNTTTTTTTTAADAQQNPTDN-------------PQNAGDGAGNRRQRTP 47
Query: 1054 FTDLSQVDADLALARTLQEQERAYMMLRMH--GGSDYG--XXXXXXXXXXXXXXXXXXXX 887
FTDLSQVDADLALARTLQEQERAYMMLRM GSDYG
Sbjct: 48 FTDLSQVDADLALARTLQEQERAYMMLRMDIANGSDYGSWEADSYGHEDEADDDDFDDAS 107
Query: 886 XXDYDGTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYARALQDXXXXXX 707
DYDG+ E ED Q ELDPSAFSSDEAYARALQD
Sbjct: 108 EEDYDGSDVEVDDDAEDAFDVHAHAEEAED-TQNAELDPSAFSSDEAYARALQDAEEREM 166
Query: 706 XXXXXXXAGINEMIVGEAEDEE--DHGDNSQDAWEEVDPDELSYEELLALGEVVGTESRG 533
AGIN M+VGE E+EE +H N QDAWEEVDPDELSYEELLALG+VVGTESRG
Sbjct: 167 AARLLALAGINGMVVGEDEEEELDEHDGNPQDAWEEVDPDELSYEELLALGDVVGTESRG 226
Query: 532 LPADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPECINNWLRI 353
L ADTIASLPS+NYKSQGIQ+GS+D+CVICRL+YEDGDTLTVLSCKHSYH ECINNWL+I
Sbjct: 227 LSADTIASLPSINYKSQGIQEGSNDTCVICRLDYEDGDTLTVLSCKHSYHSECINNWLQI 286
Query: 352 NKVCPVCSAEVVSPSGNS 299
NKVCPVCSAE VS SG S
Sbjct: 287 NKVCPVCSAE-VSTSGKS 303
>emb|CDP14406.1| unnamed protein product [Coffea canephora]
Length = 302
Score = 308 bits (788), Expect = 9e-81
Identities = 169/263 (64%), Positives = 190/263 (72%), Gaps = 5/263 (1%)
Frame = -2
Query: 1072 RRQRTPFTDLSQVDADLALARTLQEQERAYMMLRMH--GGSDYG-XXXXXXXXXXXXXXX 902
RRQRTPFTDLSQ DADLALARTLQEQERAYMMLRM+ GSDYG
Sbjct: 41 RRQRTPFTDLSQEDADLALARTLQEQERAYMMLRMNVGEGSDYGSWEGGSYGHVDDEDDD 100
Query: 901 XXXXXXXDYDGTXXXXXXXXXXXXXXXXXXEG--GEDENQVVELDPSAFSSDEAYARALQ 728
+YDG+ + ED++Q VE+DPSA+SSDEAYARALQ
Sbjct: 101 FDDPSEEEYDGSEVEEDENEEEEDAFDVNAQDEEEEDDSQNVEIDPSAYSSDEAYARALQ 160
Query: 727 DXXXXXXXXXXXXXAGINEMIVGEAEDEEDHGDNSQDAWEEVDPDELSYEELLALGEVVG 548
D AGINEM++GEA+DEE+ NSQDAWEEVDPDELSYEELLALGEVVG
Sbjct: 161 DAEEREMAARLLSMAGINEMVIGEADDEEEDDGNSQDAWEEVDPDELSYEELLALGEVVG 220
Query: 547 TESRGLPADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPECIN 368
TESRGL ADTIASLP+++YKSQ + GS+DSCVICRL+YE+GD LTVLSCKHSYHP+CIN
Sbjct: 221 TESRGLSADTIASLPAMSYKSQCTEAGSTDSCVICRLDYEEGDRLTVLSCKHSYHPDCIN 280
Query: 367 NWLRINKVCPVCSAEVVSPSGNS 299
NWL+INKVCPVCSAE VS SGNS
Sbjct: 281 NWLKINKVCPVCSAE-VSTSGNS 302
>ref|XP_009622651.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-related [Nicotiana
tomentosiformis]
Length = 331
Score = 303 bits (776), Expect = 2e-79
Identities = 166/266 (62%), Positives = 184/266 (69%), Gaps = 3/266 (1%)
Frame = -2
Query: 1087 DAEDNRRQRTPFTDLSQVDADLALARTLQEQERAYMMLRMHG-GSDYGXXXXXXXXXXXX 911
D+ +RTPFT+LSQ DADLALARTLQEQERAYMML M+G G D+G
Sbjct: 67 DSNGGASRRTPFTNLSQEDADLALARTLQEQERAYMMLSMNGDGIDFGSWDGGSYDHDEE 126
Query: 910 XXXXXXXXXXDY--DGTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYAR 737
+ D E GEDENQ +ELDPSAFSSDEAYAR
Sbjct: 127 DDEDFDDPSEEDEDDSNVDEDEEDGEDAFDVHAHDEAGEDENQGIELDPSAFSSDEAYAR 186
Query: 736 ALQDXXXXXXXXXXXXXAGINEMIVGEAEDEEDHGDNSQDAWEEVDPDELSYEELLALGE 557
ALQD AGINEM VG+A+D ED G NSQDAWE+VDPDELSYEEL+ALGE
Sbjct: 187 ALQDAEEREMAARLLALAGINEMYVGQAQDREDRGINSQDAWEDVDPDELSYEELIALGE 246
Query: 556 VVGTESRGLPADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPE 377
VVGTESRGL ADTIASLPSVNYK+Q +G++DSCVICRLEYEDGD LTVLSCKHSYH E
Sbjct: 247 VVGTESRGLSADTIASLPSVNYKAQTASEGTTDSCVICRLEYEDGDQLTVLSCKHSYHSE 306
Query: 376 CINNWLRINKVCPVCSAEVVSPSGNS 299
C+NNWL+INKVCP+CS E VS SGNS
Sbjct: 307 CLNNWLQINKVCPMCSTE-VSTSGNS 331
>ref|XP_012839641.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-related [Erythranthe
guttatus] gi|604330581|gb|EYU35575.1| hypothetical
protein MIMGU_mgv1a010240mg [Erythranthe guttata]
Length = 318
Score = 302 bits (773), Expect = 5e-79
Identities = 173/320 (54%), Positives = 195/320 (60%), Gaps = 17/320 (5%)
Frame = -2
Query: 1219 MENTNSKANTNNTCEQEDLTQNPRPGIXXXXXXXXXXXXXXXNDDAEDNRRQRTPFTDLS 1040
M + NSKA+ +T + D QNP A NR +RTPFT+LS
Sbjct: 1 MADPNSKADAVDTTVRADEEQNPTNNPPTEGDGAAAATAV-----AAANRPRRTPFTNLS 55
Query: 1039 QVDADLALARTLQEQERAYMMLRMH--GGSDYGXXXXXXXXXXXXXXXXXXXXXXDYDGT 866
QVDADLALARTLQEQERAYMMLRM GSDYG DYDG+
Sbjct: 56 QVDADLALARTLQEQERAYMMLRMDMGEGSDYGSWEAESYEDEDEVDAYDDASEEDYDGS 115
Query: 865 XXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYARALQDXXXXXXXXXXXXX 686
G E ++Q E+DPSAFSSDEAYARALQD
Sbjct: 116 DVEVDDEAEDAFDVHARAGGVESDSQNDEVDPSAFSSDEAYARALQDAEEREMAARLLAL 175
Query: 685 AGINEMIVGEA---------------EDEEDHGDNSQDAWEEVDPDELSYEELLALGEVV 551
AGIN+M+VGE EDEE+HG N+QDAWEEVDPDELSYEEL+ALG+V+
Sbjct: 176 AGINDMVVGEDDEEEDDDDDDDGEYDEDEEEHGSNAQDAWEEVDPDELSYEELIALGDVI 235
Query: 550 GTESRGLPADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPECI 371
GTESRGL AD IASLPSVNY S+G +G+ DSCVICRL+YED D LTVLSCKH YHPECI
Sbjct: 236 GTESRGLSADAIASLPSVNYNSKGTHEGNGDSCVICRLDYEDDDNLTVLSCKHLYHPECI 295
Query: 370 NNWLRINKVCPVCSAEVVSP 311
+NWL INKVCPVCSAEV P
Sbjct: 296 SNWLNINKVCPVCSAEVSIP 315
>ref|XP_009775151.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-related [Nicotiana
sylvestris]
Length = 319
Score = 301 bits (770), Expect = 1e-78
Identities = 164/256 (64%), Positives = 180/256 (70%), Gaps = 1/256 (0%)
Frame = -2
Query: 1063 RTPFTDLSQVDADLALARTLQEQERAYMMLRMHG-GSDYGXXXXXXXXXXXXXXXXXXXX 887
RTPFT+LSQ DADLALARTLQEQERAYMML M+ G D+G
Sbjct: 67 RTPFTNLSQEDADLALARTLQEQERAYMMLSMNSDGIDFGSWDGGSYDHDEEDDEDFDDP 126
Query: 886 XXDYDGTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYARALQDXXXXXX 707
+ D + GEDENQ +ELDPSAFSSDEAYARALQD
Sbjct: 127 SEEEDDSNVDEDEEDAFDVHAHDE--AGEDENQGIELDPSAFSSDEAYARALQDAEERDM 184
Query: 706 XXXXXXXAGINEMIVGEAEDEEDHGDNSQDAWEEVDPDELSYEELLALGEVVGTESRGLP 527
AGINEM VG+A+D ED G NSQDAWE+VDPDELSYEEL+ALGEVVGTESRGL
Sbjct: 185 AARLLALAGINEMYVGQAQDGEDRGINSQDAWEDVDPDELSYEELIALGEVVGTESRGLS 244
Query: 526 ADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPECINNWLRINK 347
ADTIASLPSVNYK+Q DG++DSCVICRLEYEDGD LTVLSCKHSYH EC+NNWL+INK
Sbjct: 245 ADTIASLPSVNYKAQTASDGTTDSCVICRLEYEDGDQLTVLSCKHSYHSECLNNWLQINK 304
Query: 346 VCPVCSAEVVSPSGNS 299
VCP+CS E VS SGNS
Sbjct: 305 VCPMCSTE-VSTSGNS 319
>ref|XP_004231468.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-related [Solanum
lycopersicum]
Length = 314
Score = 298 bits (762), Expect = 9e-78
Identities = 162/257 (63%), Positives = 181/257 (70%), Gaps = 1/257 (0%)
Frame = -2
Query: 1066 QRTPFTDLSQVDADLALARTLQEQERAYMMLRMHG-GSDYGXXXXXXXXXXXXXXXXXXX 890
+RTPFTDLSQVDADLALARTLQ+QERAYMML M+ G DYG
Sbjct: 60 RRTPFTDLSQVDADLALARTLQDQERAYMMLSMNDDGIDYGSWDAGNYGHDEDEDEDFDD 119
Query: 889 XXXDYDGTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYARALQDXXXXX 710
+ D E EDENQ VELDPSAFSSDEAYARALQD
Sbjct: 120 PSEEEDD-GSDVGEDDEDAFDVHAHDEASEDENQGVELDPSAFSSDEAYARALQDAEERE 178
Query: 709 XXXXXXXXAGINEMIVGEAEDEEDHGDNSQDAWEEVDPDELSYEELLALGEVVGTESRGL 530
AGINEM VG+ +DEED G NSQDAWE+VDPDELSYEEL+ALG+VVGTESRGL
Sbjct: 179 MAARLLALAGINEMYVGQRQDEEDRGINSQDAWEDVDPDELSYEELIALGDVVGTESRGL 238
Query: 529 PADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPECINNWLRIN 350
ADTIASLPSVNYK+Q +G++DSCVICRL+YEDG+ LTVLSCKH+YH EC+NNWL+IN
Sbjct: 239 SADTIASLPSVNYKTQTASEGTTDSCVICRLDYEDGEKLTVLSCKHTYHSECLNNWLQIN 298
Query: 349 KVCPVCSAEVVSPSGNS 299
KVCP+CS E VS SGNS
Sbjct: 299 KVCPICSTE-VSTSGNS 314
>ref|XP_006365590.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-related-like [Solanum
tuberosum]
Length = 313
Score = 295 bits (756), Expect = 4e-77
Identities = 174/317 (54%), Positives = 196/317 (61%), Gaps = 10/317 (3%)
Frame = -2
Query: 1219 MENTNSKANTNN------TCEQEDLTQNPRPGIXXXXXXXXXXXXXXXNDDA---EDNRR 1067
MEN+ S N NN T ++ Q+ P DA +
Sbjct: 1 MENSESNNNNNNNEAAKTTAVDDNTHQSGNPN--GSDGNASLQVNANGESDAALHQPGAS 58
Query: 1066 QRTPFTDLSQVDADLALARTLQEQERAYMMLRMHG-GSDYGXXXXXXXXXXXXXXXXXXX 890
+RTPFTDLSQVDADLALARTLQ+QERAYMML M+ G DYG
Sbjct: 59 RRTPFTDLSQVDADLALARTLQDQERAYMMLSMNDDGIDYGSWDAGSYGHDEDEDEDFDD 118
Query: 889 XXXDYDGTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYARALQDXXXXX 710
+ D E EDENQ VELDPSAFSSDEAYARALQD
Sbjct: 119 PSEEEDD-GSNVDEDDEDAFDVHAHEEASEDENQGVELDPSAFSSDEAYARALQDAEERE 177
Query: 709 XXXXXXXXAGINEMIVGEAEDEEDHGDNSQDAWEEVDPDELSYEELLALGEVVGTESRGL 530
AGINEM VG+ +DEED NSQDAWE+VDPDELSYEEL+ALGEVVGTESRGL
Sbjct: 178 MAARLLALAGINEMYVGQTQDEEDRAINSQDAWEDVDPDELSYEELIALGEVVGTESRGL 237
Query: 529 PADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPECINNWLRIN 350
ADTIASLPSV YK+Q +G++DSCVICRL+YEDGD LTVLSCKH+YH EC+NNWL+IN
Sbjct: 238 SADTIASLPSVIYKTQTASEGTTDSCVICRLDYEDGDKLTVLSCKHTYHSECLNNWLQIN 297
Query: 349 KVCPVCSAEVVSPSGNS 299
KVCP+CS E VS SGNS
Sbjct: 298 KVCPICSTE-VSTSGNS 313
>ref|XP_006382946.1| hypothetical protein POPTR_0005s09300g [Populus trichocarpa]
gi|550338462|gb|ERP60743.1| hypothetical protein
POPTR_0005s09300g [Populus trichocarpa]
Length = 316
Score = 289 bits (739), Expect = 4e-75
Identities = 166/310 (53%), Positives = 192/310 (61%), Gaps = 11/310 (3%)
Frame = -2
Query: 1216 ENTNSKANT--------NNTCEQEDLTQNPRPGIXXXXXXXXXXXXXXXNDDAEDNRRQR 1061
+N N+ +NT NN EQ++ +NP R R
Sbjct: 5 DNNNNSSNTKLGSGESKNNNSEQQE--ENPNLSSNGEQQEGDGEREGGGGGPQRQTSR-R 61
Query: 1060 TPFTDLSQVDADLALARTLQEQERAYMMLRMHG-GSDYGXXXXXXXXXXXXXXXXXXXXX 884
TPFT+LSQVDADLALARTLQEQERAYMMLRM+ GSDYG
Sbjct: 62 TPFTNLSQVDADLALARTLQEQERAYMMLRMNNDGSDYGSWEAGSYLRDDEDDFGDPDDE 121
Query: 883 XDYDGTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYARALQDXXXXXXX 704
D D + G+ +N VE+DPS +SSDEAYARALQ+
Sbjct: 122 TDEDDDTDVDEADAFDAAAAAADADAGDHDNTEVEIDPSVYSSDEAYARALQEAEEREVA 181
Query: 703 XXXXXXAGINEMIVGEAED--EEDHGDNSQDAWEEVDPDELSYEELLALGEVVGTESRGL 530
AG+N+ I GEAE+ EEDH DNSQD WEEVDPDELSYEELLALGEVVGTESRGL
Sbjct: 182 ARLLALAGLND-ITGEAEEDTEEDHEDNSQDTWEEVDPDELSYEELLALGEVVGTESRGL 240
Query: 529 PADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPECINNWLRIN 350
ADTIASLPS+NYK+ Q+GS+DSCVICRL+YEDG+TLT+LSCKHSYH ECINNWL+IN
Sbjct: 241 SADTIASLPSINYKTGSSQNGSNDSCVICRLDYEDGETLTLLSCKHSYHSECINNWLKIN 300
Query: 349 KVCPVCSAEV 320
K CPVC+ EV
Sbjct: 301 KACPVCNTEV 310
>ref|XP_010655908.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-related isoform X2 [Vitis
vinifera] gi|296085296|emb|CBI29028.3| unnamed protein
product [Vitis vinifera]
Length = 308
Score = 285 bits (728), Expect = 8e-74
Identities = 159/272 (58%), Positives = 178/272 (65%), Gaps = 13/272 (4%)
Frame = -2
Query: 1090 DDAEDNRRQRTPFTDLSQVDADLALARTLQEQERAYMMLRMHG-GSDYGXXXXXXXXXXX 914
+ A + RTPFT+LSQVDADLALARTLQEQERAYMMLR++G GSDYG
Sbjct: 45 NQAPPRQSSRTPFTNLSQVDADLALARTLQEQERAYMMLRINGEGSDYGSWEAGSYFNEE 104
Query: 913 XXXXXXXXXXXD------------YDGTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDP 770
D YDGT GED+N +E DP
Sbjct: 105 EFDDPNDGTDVDDGVDDEDEDEGEYDGTDEDAFDVHAQADD-------GEDDNPTIEFDP 157
Query: 769 SAFSSDEAYARALQDXXXXXXXXXXXXXAGINEMIVGEAEDEEDHGDNSQDAWEEVDPDE 590
+ FSSDEAYARALQ AGI++ V ED EDHG NSQD WEEVDPDE
Sbjct: 158 AVFSSDEAYARALQHIEEQEMAARLLALAGIHDHGV---EDTEDHGGNSQDTWEEVDPDE 214
Query: 589 LSYEELLALGEVVGTESRGLPADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLT 410
LSYEELLALGEV+GTESRGL +DTIASLPSV YK+Q Q+GS+DSCVICRL+YEDG+TLT
Sbjct: 215 LSYEELLALGEVIGTESRGLSSDTIASLPSVTYKAQSNQEGSNDSCVICRLDYEDGETLT 274
Query: 409 VLSCKHSYHPECINNWLRINKVCPVCSAEVVS 314
VLSCKHSYH ECINNWL+INKVCP+CS EV S
Sbjct: 275 VLSCKHSYHSECINNWLQINKVCPICSTEVSS 306
>ref|XP_006382945.1| hypothetical protein POPTR_0005s09300g [Populus trichocarpa]
gi|550338461|gb|ERP60742.1| hypothetical protein
POPTR_0005s09300g [Populus trichocarpa]
Length = 314
Score = 285 bits (728), Expect = 8e-74
Identities = 162/308 (52%), Positives = 188/308 (61%), Gaps = 9/308 (2%)
Frame = -2
Query: 1216 ENTNSKANT--------NNTCEQEDLTQNPRPGIXXXXXXXXXXXXXXXNDDAEDNRRQR 1061
+N N+ +NT NN EQ++ +NP R R
Sbjct: 5 DNNNNSSNTKLGSGESKNNNSEQQE--ENPNLSSNGEQQEGDGEREGGGGGPQRQTSR-R 61
Query: 1060 TPFTDLSQVDADLALARTLQEQERAYMMLRMHG-GSDYGXXXXXXXXXXXXXXXXXXXXX 884
TPFT+LSQVDADLALARTLQEQERAYMMLRM+ GSDYG
Sbjct: 62 TPFTNLSQVDADLALARTLQEQERAYMMLRMNNDGSDYGSWEAGSYLRDDEDDFGDPDDE 121
Query: 883 XDYDGTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYARALQDXXXXXXX 704
D D + G+ +N VE+DPS +SSDEAYARALQ+
Sbjct: 122 TDEDDDTDVDEADAFDAAAAAADADAGDHDNTEVEIDPSVYSSDEAYARALQEAEEREVA 181
Query: 703 XXXXXXAGINEMIVGEAEDEEDHGDNSQDAWEEVDPDELSYEELLALGEVVGTESRGLPA 524
AG+N+ E + EEDH DNSQD WEEVDPDELSYEELLALGEVVGTESRGL A
Sbjct: 182 ARLLALAGLNDR-EAEEDTEEDHEDNSQDTWEEVDPDELSYEELLALGEVVGTESRGLSA 240
Query: 523 DTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPECINNWLRINKV 344
DTIASLPS+NYK+ Q+GS+DSCVICRL+YEDG+TLT+LSCKHSYH ECINNWL+INK
Sbjct: 241 DTIASLPSINYKTGSSQNGSNDSCVICRLDYEDGETLTLLSCKHSYHSECINNWLKINKA 300
Query: 343 CPVCSAEV 320
CPVC+ EV
Sbjct: 301 CPVCNTEV 308
>ref|XP_010655907.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-related isoform X1 [Vitis
vinifera]
Length = 314
Score = 284 bits (727), Expect = 1e-73
Identities = 158/275 (57%), Positives = 178/275 (64%), Gaps = 16/275 (5%)
Frame = -2
Query: 1090 DDAEDNRRQRTPFTDLSQVDADLALARTLQEQERAYMMLRMHG-GSDYGXXXXXXXXXXX 914
+ A + RTPFT+LSQVDADLALARTLQEQERAYMMLR++G GSDYG
Sbjct: 45 NQAPPRQSSRTPFTNLSQVDADLALARTLQEQERAYMMLRINGEGSDYGSWEAGSYFNEE 104
Query: 913 XXXXXXXXXXXD------------YDGTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDP 770
D YDGT GED+N +E DP
Sbjct: 105 EFDDPNDGTDVDDGVDDEDEDEGEYDGTDEDAFDVHAQADD-------GEDDNPTIEFDP 157
Query: 769 SAFSSDEAYARALQDXXXXXXXXXXXXXAGINE---MIVGEAEDEEDHGDNSQDAWEEVD 599
+ FSSDEAYARALQ AGI++ + ED EDHG NSQD WEEVD
Sbjct: 158 AVFSSDEAYARALQHIEEQEMAARLLALAGIHDRECLFRNGVEDTEDHGGNSQDTWEEVD 217
Query: 598 PDELSYEELLALGEVVGTESRGLPADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGD 419
PDELSYEELLALGEV+GTESRGL +DTIASLPSV YK+Q Q+GS+DSCVICRL+YEDG+
Sbjct: 218 PDELSYEELLALGEVIGTESRGLSSDTIASLPSVTYKAQSNQEGSNDSCVICRLDYEDGE 277
Query: 418 TLTVLSCKHSYHPECINNWLRINKVCPVCSAEVVS 314
TLTVLSCKHSYH ECINNWL+INKVCP+CS EV S
Sbjct: 278 TLTVLSCKHSYHSECINNWLQINKVCPICSTEVSS 312
>ref|XP_009366271.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-related isoform X1 [Pyrus
x bretschneideri]
Length = 320
Score = 282 bits (722), Expect = 4e-73
Identities = 158/263 (60%), Positives = 177/263 (67%), Gaps = 8/263 (3%)
Frame = -2
Query: 1063 RTPFTDLSQVDADLALARTLQEQERAYMMLRMHG-GSDYGXXXXXXXXXXXXXXXXXXXX 887
RTPF++LSQVDADLALARTLQEQERAYMMLRM+ GSDYG
Sbjct: 65 RTPFSNLSQVDADLALARTLQEQERAYMMLRMNNEGSDYGSWEAGSYVHEDDDDFNEFDD 124
Query: 886 XXD-------YDGTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYARALQ 728
D YDGT GED+N +VE P+ FSSDEAYARALQ
Sbjct: 125 DADDNDDEEQYDGTEVDNDEDAFNLHAHDD---AGEDDNPIVEYGPAVFSSDEAYARALQ 181
Query: 727 DXXXXXXXXXXXXXAGINEMIVGEAEDEEDHGDNSQDAWEEVDPDELSYEELLALGEVVG 548
D +GIN+ E ED E+H NSQD WEE+DPDELSYEELLALGEVVG
Sbjct: 182 DAEDREMAARLMALSGINDR---EVEDTEEHHGNSQDTWEEIDPDELSYEELLALGEVVG 238
Query: 547 TESRGLPADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPECIN 368
TESRGL ADTIASLPSV+YK+ Q+GS++SCVICRL++EDG+ LT+LSCKHSYH ECIN
Sbjct: 239 TESRGLSADTIASLPSVSYKTGSSQNGSNESCVICRLDFEDGENLTILSCKHSYHSECIN 298
Query: 367 NWLRINKVCPVCSAEVVSPSGNS 299
NWL INKVCPVCSAE VS SGNS
Sbjct: 299 NWLTINKVCPVCSAE-VSTSGNS 320
>ref|XP_010261280.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-related [Nelumbo nucifera]
Length = 278
Score = 282 bits (721), Expect = 5e-73
Identities = 157/257 (61%), Positives = 168/257 (65%), Gaps = 2/257 (0%)
Frame = -2
Query: 1084 AEDNRRQRTPFTDLSQVDADLALARTLQEQERAYMMLRMHGG--SDYGXXXXXXXXXXXX 911
A + RTPFT LSQVDADLALARTLQEQERAYMMLRM+ G SDYG
Sbjct: 31 AASRQTSRTPFTSLSQVDADLALARTLQEQERAYMMLRMNSGEGSDYGSSENGSYVYEDD 90
Query: 910 XXXXXXXXXXDYDGTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYARAL 731
+ D D NQ ELDPSAF SDEAYARAL
Sbjct: 91 VDDQNEEENAETDADEDAFDVHARVDAG---------DNNQATELDPSAFDSDEAYARAL 141
Query: 730 QDXXXXXXXXXXXXXAGINEMIVGEAEDEEDHGDNSQDAWEEVDPDELSYEELLALGEVV 551
Q+ GIN+ EAED EDHG NSQD WEEVDPDELSYEELLALGEVV
Sbjct: 142 QEAEMAVHLMALA---GINDW---EAEDSEDHGSNSQDTWEEVDPDELSYEELLALGEVV 195
Query: 550 GTESRGLPADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPECI 371
GTESRGL AD IASLPSV+Y +Q QDGS+D CVICRL+YEDG+TLTVLSCKHSYH ECI
Sbjct: 196 GTESRGLSADMIASLPSVSYNAQSNQDGSTDQCVICRLDYEDGETLTVLSCKHSYHSECI 255
Query: 370 NNWLRINKVCPVCSAEV 320
NWL+INKVCPVCSAEV
Sbjct: 256 TNWLQINKVCPVCSAEV 272
>ref|XP_008383690.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-related-like isoform X1
[Malus domestica]
Length = 315
Score = 281 bits (718), Expect = 1e-72
Identities = 157/259 (60%), Positives = 177/259 (68%), Gaps = 4/259 (1%)
Frame = -2
Query: 1063 RTPFTDLSQVDADLALARTLQEQERAYMMLRMHG-GSDYGXXXXXXXXXXXXXXXXXXXX 887
RTPF++LSQVDADLALARTLQEQERAYMMLRM+ GSDYG
Sbjct: 64 RTPFSNLSQVDADLALARTLQEQERAYMMLRMNNEGSDYGSWEAGSYVHEDDNDFNEFDD 123
Query: 886 XXD---YDGTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYARALQDXXX 716
D YDGT GED+N VE P+ SSDEAYARALQD
Sbjct: 124 DADEEQYDGTEVDSDEDAFDLHAXDD---AGEDDNPSVEYGPAVXSSDEAYARALQDAED 180
Query: 715 XXXXXXXXXXAGINEMIVGEAEDEEDHGDNSQDAWEEVDPDELSYEELLALGEVVGTESR 536
+GIN+ E ED E+HG NSQD WEE+DPDELSYEELLALGEVVGTE+R
Sbjct: 181 REMAARLMALSGINDR---EVEDTEEHGGNSQDTWEEIDPDELSYEELLALGEVVGTENR 237
Query: 535 GLPADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPECINNWLR 356
GL ADTIASLPSV+YK+ Q+GS++SCVICRL++EDG+ LT+LSCKHSYH ECINNWL
Sbjct: 238 GLSADTIASLPSVSYKTGSSQNGSNESCVICRLDFEDGENLTILSCKHSYHSECINNWLT 297
Query: 355 INKVCPVCSAEVVSPSGNS 299
INK+CPVCSAE VS SGNS
Sbjct: 298 INKICPVCSAE-VSTSGNS 315
>ref|XP_007043263.1| RING/U-box superfamily protein [Theobroma cacao]
gi|508707198|gb|EOX99094.1| RING/U-box superfamily
protein [Theobroma cacao]
Length = 392
Score = 281 bits (718), Expect = 1e-72
Identities = 167/312 (53%), Positives = 189/312 (60%), Gaps = 15/312 (4%)
Frame = -2
Query: 1195 NTNNTCEQEDLTQNPRPGIXXXXXXXXXXXXXXXNDDAEDNRRQ---RTPFTDLSQVDAD 1025
N N + E ++ NP ++ E++ RQ RTPFT+LSQVDAD
Sbjct: 87 NANMSSEPKEQEPNPNGNDNDNLNNESEHVEREEEEEEEEDTRQSSTRTPFTNLSQVDAD 146
Query: 1024 LALARTLQEQERAYMMLRMHG-GSDYGXXXXXXXXXXXXXXXXXXXXXXD---------- 878
LALARTLQEQERAYMMLRM+ GSDYG D
Sbjct: 147 LALARTLQEQERAYMMLRMNNDGSDYGSWEAGSYLHDDDDDFGDPHDHDDTDGDEEEDEE 206
Query: 877 -YDGTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYARALQDXXXXXXXX 701
YDGT G ED N VE +P+ FSSDEAYARALQD
Sbjct: 207 EYDGTAADDDVDAFDVHAHAEN--GEEDYNPNVEFNPAVFSSDEAYARALQDAEEREMAA 264
Query: 700 XXXXXAGINEMIVGEAEDEEDHGDNSQDAWEEVDPDELSYEELLALGEVVGTESRGLPAD 521
AG+N+ + ED HG NSQD WEEVDPDELSYEELLALGEVVGTESRGL AD
Sbjct: 265 RLLALAGLNDRGIVTLED---HGGNSQDTWEEVDPDELSYEELLALGEVVGTESRGLSAD 321
Query: 520 TIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPECINNWLRINKVC 341
+IASLPSV YK+ Q GS+DSCV+CR++YEDGD+LTVLSCKHSYHPECINNWL+INKVC
Sbjct: 322 SIASLPSVTYKAGSSQTGSNDSCVVCRVDYEDGDSLTVLSCKHSYHPECINNWLKINKVC 381
Query: 340 PVCSAEVVSPSG 305
PVCSAE VS SG
Sbjct: 382 PVCSAE-VSTSG 392
>ref|XP_004497226.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-related isoform X1 [Cicer
arietinum]
Length = 342
Score = 281 bits (718), Expect = 1e-72
Identities = 154/264 (58%), Positives = 174/264 (65%), Gaps = 9/264 (3%)
Frame = -2
Query: 1063 RTPFTDLSQVDADLALARTLQEQERAYMMLRMHG-GSDYGXXXXXXXXXXXXXXXXXXXX 887
RTPFT+LSQVDADLALARTLQEQERAYMMLRM+ GSDYG
Sbjct: 78 RTPFTNLSQVDADLALARTLQEQERAYMMLRMNNDGSDYGSWEGGSYLHDDGDDFDDLHD 137
Query: 886 XXDYD-----GTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYARALQDX 722
D D GE +N ++E DP FSSDEAYARALQ+
Sbjct: 138 GTDVDEDDDEDDDNEDEYENEDAFDVHAHASAGEHDNPIIEFDPELFSSDEAYARALQEA 197
Query: 721 XXXXXXXXXXXXAGINEM---IVGEAEDEEDHGDNSQDAWEEVDPDELSYEELLALGEVV 551
AGIN++ I E ED E+HG NSQDAWE+VDPDELSYEELLALGEVV
Sbjct: 198 EEREMAARLLALAGINDLSFSITEETEDMEEHGANSQDAWEDVDPDELSYEELLALGEVV 257
Query: 550 GTESRGLPADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPECI 371
GTESRGL DTIA LPSVNYK+ Q GS+DSCVICR++YED ++LTVLSCKH YHPECI
Sbjct: 258 GTESRGLSTDTIACLPSVNYKTGSDQHGSNDSCVICRVDYEDDESLTVLSCKHLYHPECI 317
Query: 370 NNWLRINKVCPVCSAEVVSPSGNS 299
NNWL+INKVCPVCS EV + SG++
Sbjct: 318 NNWLKINKVCPVCSTEVSASSGSN 341
>ref|XP_004287200.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-related [Fragaria vesca
subsp. vesca]
Length = 306
Score = 280 bits (717), Expect = 1e-72
Identities = 168/312 (53%), Positives = 186/312 (59%), Gaps = 5/312 (1%)
Frame = -2
Query: 1219 MENTNSKANTNNTCEQEDLTQNPRPGIXXXXXXXXXXXXXXXNDDAEDNRRQRTPFTDLS 1040
MEN N+K + + Q P + + RTPFT+LS
Sbjct: 1 MENHNAKLVPSGGLKPNPGEQEENPTLNRCGEEEEVNTESAEPPSPNAPQSSRTPFTNLS 60
Query: 1039 QVDADLALARTLQEQERAYMMLRMHG-GSDYGXXXXXXXXXXXXXXXXXXXXXXD----Y 875
QVDADLALARTLQEQERAYMMLRM+ GSDYG D Y
Sbjct: 61 QVDADLALARTLQEQERAYMMLRMNNEGSDYGSWEAGSYVHDDEDDFHDDTDGNDDDEHY 120
Query: 874 DGTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYARALQDXXXXXXXXXX 695
DGT G+D N V DP+ FSSDEAYARALQD
Sbjct: 121 DGTDADNDEEDAFDVHAHED--AGDDHNPSVVYDPAVFSSDEAYARALQDAEDRDMAARL 178
Query: 694 XXXAGINEMIVGEAEDEEDHGDNSQDAWEEVDPDELSYEELLALGEVVGTESRGLPADTI 515
AGI++ EAED E+ G NSQDAWEEVDPDELSYEELLALGEVVGTESRGLPADTI
Sbjct: 179 MAFAGIDDR---EAEDTENQGGNSQDAWEEVDPDELSYEELLALGEVVGTESRGLPADTI 235
Query: 514 ASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPECINNWLRINKVCPV 335
ASLPSV+YK Q+GS++SC ICRL+YEDG+ LTVLSC HSYH ECINNWL INKVCPV
Sbjct: 236 ASLPSVSYKMGNGQNGSNESCAICRLDYEDGENLTVLSCTHSYHSECINNWLTINKVCPV 295
Query: 334 CSAEVVSPSGNS 299
C AE VS SGNS
Sbjct: 296 CIAE-VSTSGNS 306
>ref|XP_008243095.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-related [Prunus mume]
Length = 316
Score = 280 bits (715), Expect = 3e-72
Identities = 173/325 (53%), Positives = 193/325 (59%), Gaps = 18/325 (5%)
Frame = -2
Query: 1219 MENTNSKA--------NTNNTCEQEDLTQNPRPGIXXXXXXXXXXXXXXXNDDAEDNRRQ 1064
MEN N+K N NN+ +++ QNP D Q
Sbjct: 1 MENHNAKLSSGGGLKPNNNNSINEQE--QNPNLNQCEEQEQEVTTESSQLVDPPSPPVPQ 58
Query: 1063 --RTPFTDLSQVDADLALARTLQEQERAYMMLRMHG-GSDYGXXXXXXXXXXXXXXXXXX 893
RTPFT+LSQVDADLALARTLQEQERAYMMLRM+ SDYG
Sbjct: 59 SSRTPFTNLSQVDADLALARTLQEQERAYMMLRMNNEDSDYGSWEAGSYVHEDEDDFNDF 118
Query: 892 XXXXD-------YDGTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYARA 734
D YDGT GED+N VE DP+ FSSDEAYARA
Sbjct: 119 HDDTDDNDDEEQYDGTEVDNDEDAFDVHAHDD---AGEDDNPRVEYDPAVFSSDEAYARA 175
Query: 733 LQDXXXXXXXXXXXXXAGINEMIVGEAEDEEDHGDNSQDAWEEVDPDELSYEELLALGEV 554
LQD AGIN+ E E E+HG NSQD WEEVDPDELSYEEL+ALGEV
Sbjct: 176 LQDAEDREMAARLMALAGINDR---EVEHTEEHGGNSQDTWEEVDPDELSYEELIALGEV 232
Query: 553 VGTESRGLPADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPEC 374
VGTESRGL ADTIASLPSV+YK+ Q+GS++SCVICRL+YE G+ LT+LSCKHSYH EC
Sbjct: 233 VGTESRGLSADTIASLPSVSYKTGSGQNGSNESCVICRLDYEAGENLTILSCKHSYHSEC 292
Query: 373 INNWLRINKVCPVCSAEVVSPSGNS 299
INNWL INKVCPVCSAE VS SGNS
Sbjct: 293 INNWLTINKVCPVCSAE-VSTSGNS 316
>ref|XP_007202272.1| hypothetical protein PRUPE_ppa008876mg [Prunus persica]
gi|462397803|gb|EMJ03471.1| hypothetical protein
PRUPE_ppa008876mg [Prunus persica]
Length = 316
Score = 280 bits (715), Expect = 3e-72
Identities = 175/326 (53%), Positives = 192/326 (58%), Gaps = 19/326 (5%)
Frame = -2
Query: 1219 MENTNSKA---------NTNNTCEQEDLTQNPRPGIXXXXXXXXXXXXXXXNDDAEDNRR 1067
MEN N+K N N+ EQE QNP D
Sbjct: 1 MENHNAKLGSGGGLKPNNNNSISEQE---QNPNLNQCEEQEQEVTTESSQLVDPPSPPVP 57
Query: 1066 Q--RTPFTDLSQVDADLALARTLQEQERAYMMLRMHG-GSDYGXXXXXXXXXXXXXXXXX 896
Q RTPFT+LSQVDADLALARTLQEQERAYMMLRM+ SDYG
Sbjct: 58 QSSRTPFTNLSQVDADLALARTLQEQERAYMMLRMNNEDSDYGSWEAGSYVHEDEDDFND 117
Query: 895 XXXXXD-------YDGTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYAR 737
D YDGT GED+N VE DP+ FSSDEAYAR
Sbjct: 118 FHDDTDDNDDEEQYDGTEVDNDEDAFDVHGHDD---AGEDDNPRVEYDPAVFSSDEAYAR 174
Query: 736 ALQDXXXXXXXXXXXXXAGINEMIVGEAEDEEDHGDNSQDAWEEVDPDELSYEELLALGE 557
ALQD AGIN+ E E E+HG NSQD WEEVDPDELSYEEL+ALGE
Sbjct: 175 ALQDAEDREMAARLMALAGINDR---EVEHTEEHGGNSQDTWEEVDPDELSYEELIALGE 231
Query: 556 VVGTESRGLPADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPE 377
VVGTESRGL ADTIASLPSV+YK+ Q+GS++SCVICRL+YE G+ LT+LSCKHSYH E
Sbjct: 232 VVGTESRGLSADTIASLPSVSYKTGSGQNGSNESCVICRLDYEAGENLTILSCKHSYHSE 291
Query: 376 CINNWLRINKVCPVCSAEVVSPSGNS 299
CINNWL INKVCPVCSAE VS SGNS
Sbjct: 292 CINNWLTINKVCPVCSAE-VSTSGNS 316
>ref|XP_004497227.1| PREDICTED: E3 ubiquitin ligase BIG BROTHER-related isoform X2 [Cicer
arietinum]
Length = 336
Score = 279 bits (714), Expect = 3e-72
Identities = 153/261 (58%), Positives = 172/261 (65%), Gaps = 6/261 (2%)
Frame = -2
Query: 1063 RTPFTDLSQVDADLALARTLQEQERAYMMLRMHG-GSDYGXXXXXXXXXXXXXXXXXXXX 887
RTPFT+LSQVDADLALARTLQEQERAYMMLRM+ GSDYG
Sbjct: 78 RTPFTNLSQVDADLALARTLQEQERAYMMLRMNNDGSDYGSWEGGSYLHDDGDDFDDLHD 137
Query: 886 XXDYD-----GTXXXXXXXXXXXXXXXXXXEGGEDENQVVELDPSAFSSDEAYARALQDX 722
D D GE +N ++E DP FSSDEAYARALQ+
Sbjct: 138 GTDVDEDDDEDDDNEDEYENEDAFDVHAHASAGEHDNPIIEFDPELFSSDEAYARALQEA 197
Query: 721 XXXXXXXXXXXXAGINEMIVGEAEDEEDHGDNSQDAWEEVDPDELSYEELLALGEVVGTE 542
AGIN+ E ED E+HG NSQDAWE+VDPDELSYEELLALGEVVGTE
Sbjct: 198 EEREMAARLLALAGINDQ---ETEDMEEHGANSQDAWEDVDPDELSYEELLALGEVVGTE 254
Query: 541 SRGLPADTIASLPSVNYKSQGIQDGSSDSCVICRLEYEDGDTLTVLSCKHSYHPECINNW 362
SRGL DTIA LPSVNYK+ Q GS+DSCVICR++YED ++LTVLSCKH YHPECINNW
Sbjct: 255 SRGLSTDTIACLPSVNYKTGSDQHGSNDSCVICRVDYEDDESLTVLSCKHLYHPECINNW 314
Query: 361 LRINKVCPVCSAEVVSPSGNS 299
L+INKVCPVCS EV + SG++
Sbjct: 315 LKINKVCPVCSTEVSASSGSN 335