BLASTX nr result
ID: Ephedra25_contig00026083
seq
BLASTX 2.2.25 [Feb-01-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Ephedra25_contig00026083 (585 letters) Database: ./nr 37,332,560 sequences; 13,225,080,153 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|XP_006447346.1| hypothetical protein CICLE_v10016270mg [Citr... 147 2e-33 ref|XP_004139938.1| PREDICTED: expansin-B3-like [Cucumis sativus] 143 3e-32 gb|AAB61709.1| beta-expansin [Arabidopsis thaliana] 143 3e-32 ref|NP_179668.1| expansin B1 [Arabidopsis thaliana] gi|20138056|... 143 3e-32 ref|XP_002884220.1| beta-expansin [Arabidopsis lyrata subsp. lyr... 143 3e-32 ref|XP_002319359.2| Beta-expansin 1 precursor family protein [Po... 142 5e-32 ref|XP_002267127.2| PREDICTED: expansin-B3-like [Vitis vinifera]... 142 5e-32 ref|XP_002517939.1| Beta-expansin 3 precursor, putative [Ricinus... 142 5e-32 emb|CAN83473.1| hypothetical protein VITISV_004796 [Vitis vinifera] 142 5e-32 gb|AAZ08314.1| putative beta-expansin [Eucalyptus globulus] 142 6e-32 gb|EOX99394.1| Expansin B3, BETA 1.6 isoform 1 [Theobroma cacao] 141 1e-31 gb|ACU86966.1| expansin B1 [Raphanus sativus] gi|256561135|gb|AC... 141 1e-31 ref|XP_006357491.1| PREDICTED: expansin-B16-like [Solanum tubero... 141 1e-31 ref|XP_004243343.1| PREDICTED: expansin-B3-like [Solanum lycoper... 141 1e-31 ref|XP_006412979.1| hypothetical protein EUTSA_v10025985mg [Eutr... 141 1e-31 ref|XP_006371575.1| hypothetical protein POPTR_0019s13300g [Popu... 140 2e-31 ref|XP_002329415.1| hypothetical protein POPTRDRAFT_836962 [Popu... 140 2e-31 ref|XP_004136819.1| PREDICTED: expansin-B3-like [Cucumis sativus] 140 2e-31 ref|NP_567803.1| expansin B3 [Arabidopsis thaliana] gi|20138391|... 140 2e-31 ref|NP_001078454.1| expansin B3 [Arabidopsis thaliana] gi|332660... 140 2e-31 >ref|XP_006447346.1| hypothetical protein CICLE_v10016270mg [Citrus clementina] gi|568877224|ref|XP_006491643.1| PREDICTED: expansin-B3-like [Citrus sinensis] gi|557549957|gb|ESR60586.1| hypothetical protein CICLE_v10016270mg [Citrus clementina] Length = 268 Score = 147 bits (371), Expect = 2e-33 Identities = 64/97 (65%), Positives = 77/97 (79%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VNEGSTDYW S+L+E+EDGDGDVGS+ IREA SSEW+ M +WGANW +IGGP K Sbjct: 170 IAFHVNEGSTDYWLSLLVEFEDGDGDVGSMHIREASSSEWLQMNHVWGANWCIIGGPLKG 229 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++TTLST T+S R VIP+NW P A Y S LNF Sbjct: 230 PFSVKLTTLSTGRTLSARAVIPSNWSPKATYTSRLNF 266 >ref|XP_004139938.1| PREDICTED: expansin-B3-like [Cucumis sativus] Length = 269 Score = 143 bits (361), Expect = 3e-32 Identities = 60/97 (61%), Positives = 75/97 (77%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VNEGSTDYW S+L+E+EDGDGD+G++QI+E S EW+ M LWGANW +IGGP K Sbjct: 171 IAFHVNEGSTDYWLSLLVEFEDGDGDIGAMQIKETNSGEWLDMNHLWGANWCIIGGPLKG 230 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+R+TTLST T+S R++IP NW P A Y S L F Sbjct: 231 PFSVRLTTLSTGRTLSARDIIPRNWSPKATYTSRLKF 267 >gb|AAB61709.1| beta-expansin [Arabidopsis thaliana] Length = 259 Score = 143 bits (361), Expect = 3e-32 Identities = 61/97 (62%), Positives = 78/97 (80%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VN GSTDYW S+LIEYEDG+GD+GS+ IR+AGS EW+SMK +WGANW ++ GP K Sbjct: 159 IAFHVNAGSTDYWLSLLIEYEDGEGDIGSMHIRQAGSKEWISMKHIWGANWCIVEGPLKG 218 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++TTLS N+T+S +VIP+NW P A Y S LNF Sbjct: 219 PFSVKLTTLSNNKTLSATDVIPSNWVPKATYTSRLNF 255 >ref|NP_179668.1| expansin B1 [Arabidopsis thaliana] gi|20138056|sp|Q9SKU2.1|EXPB1_ARATH RecName: Full=Expansin-B1; Short=At-EXPB1; Short=AtEXPB1; AltName: Full=Ath-ExpBeta-1.5; AltName: Full=Beta-expansin-1; Flags: Precursor gi|4454473|gb|AAD20920.1| beta-expansin pollen allergen protein [Arabidopsis thaliana] gi|21536717|gb|AAM61049.1| beta-expansin 1 precursor (At-EXPB1) (Ath-ExpBeta-1.5) [Arabidopsis thaliana] gi|28393090|gb|AAO41979.1| putative beta-expansin pollen allergen protein [Arabidopsis thaliana] gi|28827426|gb|AAO50557.1| putative beta-expansin pollen allergen protein [Arabidopsis thaliana] gi|330251972|gb|AEC07066.1| expansin B1 [Arabidopsis thaliana] Length = 271 Score = 143 bits (361), Expect = 3e-32 Identities = 61/97 (62%), Positives = 78/97 (80%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VN GSTDYW S+LIEYEDG+GD+GS+ IR+AGS EW+SMK +WGANW ++ GP K Sbjct: 171 IAFHVNAGSTDYWLSLLIEYEDGEGDIGSMHIRQAGSKEWISMKHIWGANWCIVEGPLKG 230 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++TTLS N+T+S +VIP+NW P A Y S LNF Sbjct: 231 PFSVKLTTLSNNKTLSATDVIPSNWVPKATYTSRLNF 267 >ref|XP_002884220.1| beta-expansin [Arabidopsis lyrata subsp. lyrata] gi|297330060|gb|EFH60479.1| beta-expansin [Arabidopsis lyrata subsp. lyrata] Length = 271 Score = 143 bits (361), Expect = 3e-32 Identities = 61/97 (62%), Positives = 78/97 (80%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VN GSTDYW S+LIEYEDG+GD+GS+ IR+AGS EW+SMK +WGANW ++ GP K Sbjct: 171 IAFHVNAGSTDYWLSLLIEYEDGEGDIGSMHIRQAGSKEWISMKHIWGANWCIVEGPLKG 230 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++TTLS N+T+S +VIP+NW P A Y S LNF Sbjct: 231 PFSVKLTTLSNNKTLSAADVIPSNWVPKATYTSRLNF 267 >ref|XP_002319359.2| Beta-expansin 1 precursor family protein [Populus trichocarpa] gi|550325796|gb|EEE95282.2| Beta-expansin 1 precursor family protein [Populus trichocarpa] Length = 262 Score = 142 bits (359), Expect = 5e-32 Identities = 63/97 (64%), Positives = 75/97 (77%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VNEGSTDYW S+L+E+EDGDGDVGS+ IREAG +EW+ M LWGANW +I GP K Sbjct: 164 IAFHVNEGSTDYWLSLLVEFEDGDGDVGSMHIREAGGTEWLEMNHLWGANWCVIRGPLKG 223 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P S+++TTLST T+S R+VIP NW P A Y S LNF Sbjct: 224 PLSVKLTTLSTGRTLSARDVIPRNWVPKATYTSRLNF 260 >ref|XP_002267127.2| PREDICTED: expansin-B3-like [Vitis vinifera] gi|297739116|emb|CBI28767.3| unnamed protein product [Vitis vinifera] Length = 259 Score = 142 bits (359), Expect = 5e-32 Identities = 61/97 (62%), Positives = 76/97 (78%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VNEGSTDYW S+L+E+EDGDGDVGS+ IREA S+EW+ M+ +WGA+W + GGP K Sbjct: 162 IAFHVNEGSTDYWLSLLVEFEDGDGDVGSMHIREASSTEWLEMRHVWGASWCITGGPLKG 221 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++TTLST T+S R VIP NW P A Y S LNF Sbjct: 222 PFSVKLTTLSTGRTLSAREVIPRNWAPKATYTSRLNF 258 >ref|XP_002517939.1| Beta-expansin 3 precursor, putative [Ricinus communis] gi|223542921|gb|EEF44457.1| Beta-expansin 3 precursor, putative [Ricinus communis] Length = 259 Score = 142 bits (359), Expect = 5e-32 Identities = 62/97 (63%), Positives = 75/97 (77%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VNEGST+YW S+L+E+EDGDGDVGS+ IREAG SEW+ M LWGANW +IGGP K Sbjct: 160 IAFHVNEGSTNYWLSLLVEFEDGDGDVGSMHIREAGGSEWLEMNHLWGANWCIIGGPLKG 219 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++TTLST +S R+VIP NW P A Y S L F Sbjct: 220 PFSVKLTTLSTGRGLSARDVIPRNWSPKATYTSRLKF 256 >emb|CAN83473.1| hypothetical protein VITISV_004796 [Vitis vinifera] Length = 219 Score = 142 bits (359), Expect = 5e-32 Identities = 61/97 (62%), Positives = 76/97 (78%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VNEGSTDYW S+L+E+EDGDGDVGS+ IREA S+EW+ M+ +WGA+W + GGP K Sbjct: 122 IAFHVNEGSTDYWLSLLVEFEDGDGDVGSMHIREASSTEWLEMRHVWGASWCITGGPLKG 181 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++TTLST T+S R VIP NW P A Y S LNF Sbjct: 182 PFSVKLTTLSTGRTLSAREVIPRNWAPKATYTSRLNF 218 >gb|AAZ08314.1| putative beta-expansin [Eucalyptus globulus] Length = 207 Score = 142 bits (358), Expect = 6e-32 Identities = 62/97 (63%), Positives = 75/97 (77%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VN GSTDYW S+L+E+EDGDGDVGS+ IR A SSEW+ MK LWGANW +IGGP + Sbjct: 110 IAFHVNGGSTDYWLSLLVEFEDGDGDVGSMHIRPASSSEWIEMKHLWGANWCIIGGPLQG 169 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++T+LSTN T+S R VIP W P A Y S LNF Sbjct: 170 PFSVKLTSLSTNRTLSAREVIPGKWSPKATYTSRLNF 206 >gb|EOX99394.1| Expansin B3, BETA 1.6 isoform 1 [Theobroma cacao] Length = 266 Score = 141 bits (356), Expect = 1e-31 Identities = 61/97 (62%), Positives = 75/97 (77%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VNEGSTDYW S+L+E+EDGDGDVGS+ IREA S+EW+ M LWGANW +I GP K Sbjct: 169 IAFHVNEGSTDYWLSLLVEFEDGDGDVGSMHIREANSNEWLEMNHLWGANWCIIRGPLKG 228 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++TTL+T +S R+VIP NW P A Y S LNF Sbjct: 229 PFSVKLTTLATGRALSARDVIPRNWSPKATYTSRLNF 265 >gb|ACU86966.1| expansin B1 [Raphanus sativus] gi|256561135|gb|ACU86967.1| expansin B1 [Raphanus sativus] Length = 263 Score = 141 bits (356), Expect = 1e-31 Identities = 57/97 (58%), Positives = 78/97 (80%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VNEGSTD+W S+L+E+EDG+GD+GS+ IR+AG+SEW+ MK +WGANW ++GGP K Sbjct: 163 IAFHVNEGSTDFWLSLLVEFEDGEGDIGSMHIRQAGASEWLEMKHVWGANWCIVGGPLKG 222 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++TTLS +T+S +V+P NW P A Y S LNF Sbjct: 223 PFSIKLTTLSAGKTLSATDVVPRNWAPKATYTSRLNF 259 >ref|XP_006357491.1| PREDICTED: expansin-B16-like [Solanum tuberosum] Length = 267 Score = 141 bits (355), Expect = 1e-31 Identities = 61/97 (62%), Positives = 76/97 (78%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VNEGST YW S+L+E+EDGDGDVGS+ IREA S++W+ M LWGANWA+IGGP + Sbjct: 171 IAFHVNEGSTPYWLSLLVEFEDGDGDVGSMHIREARSNQWIEMSHLWGANWAIIGGPMQG 230 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++TTLST +S R+VIP NW P A Y S LNF Sbjct: 231 PFSVKLTTLSTGRALSARDVIPGNWSPKATYTSRLNF 267 >ref|XP_004243343.1| PREDICTED: expansin-B3-like [Solanum lycopersicum] Length = 267 Score = 141 bits (355), Expect = 1e-31 Identities = 61/97 (62%), Positives = 76/97 (78%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VNEGST YW S+L+E+EDGDGDVGS+ IREA S++W+ M LWGANWA+IGGP + Sbjct: 171 IAFHVNEGSTPYWLSLLVEFEDGDGDVGSMHIREARSNQWIEMSHLWGANWAIIGGPLQG 230 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++TTLST +S R+VIP NW P A Y S LNF Sbjct: 231 PFSVKLTTLSTGRALSARDVIPGNWSPKATYTSRLNF 267 >ref|XP_006412979.1| hypothetical protein EUTSA_v10025985mg [Eutrema salsugineum] gi|312282837|dbj|BAJ34284.1| unnamed protein product [Thellungiella halophila] gi|557114149|gb|ESQ54432.1| hypothetical protein EUTSA_v10025985mg [Eutrema salsugineum] Length = 266 Score = 141 bits (355), Expect = 1e-31 Identities = 58/97 (59%), Positives = 78/97 (80%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VNEGSTD+W S+L+E+EDG+GD+GS+ IR+AGS EW+ MK +WGANW +IGGP K Sbjct: 166 IAFHVNEGSTDFWLSLLVEFEDGEGDIGSMHIRQAGSMEWLEMKHVWGANWCIIGGPLKG 225 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++TTLS+ +T+S +V+P NW P A Y S LNF Sbjct: 226 PFSVKLTTLSSGKTLSATDVVPRNWAPKATYTSRLNF 262 >ref|XP_006371575.1| hypothetical protein POPTR_0019s13300g [Populus trichocarpa] gi|550317454|gb|ERP49372.1| hypothetical protein POPTR_0019s13300g [Populus trichocarpa] Length = 262 Score = 140 bits (354), Expect = 2e-31 Identities = 60/97 (61%), Positives = 74/97 (76%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VNEGSTDYW S+L+E+EDGDGDVGS+ IREAG +EW+ M +WGA W +I GP K Sbjct: 164 IAFHVNEGSTDYWLSILVEFEDGDGDVGSMHIREAGGTEWLEMNHVWGATWCIIRGPLKG 223 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++TTL+T T+S R VIP NW P A Y S LNF Sbjct: 224 PFSVKLTTLTTGRTLSAREVIPRNWAPKATYTSRLNF 260 >ref|XP_002329415.1| hypothetical protein POPTRDRAFT_836962 [Populus trichocarpa] Length = 267 Score = 140 bits (354), Expect = 2e-31 Identities = 60/97 (61%), Positives = 74/97 (76%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VNEGSTDYW S+L+E+EDGDGDVGS+ IREAG +EW+ M +WGA W +I GP K Sbjct: 169 IAFHVNEGSTDYWLSILVEFEDGDGDVGSMHIREAGGTEWLEMNHVWGATWCIIRGPLKG 228 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++TTL+T T+S R VIP NW P A Y S LNF Sbjct: 229 PFSVKLTTLTTGRTLSAREVIPRNWAPKATYTSRLNF 265 >ref|XP_004136819.1| PREDICTED: expansin-B3-like [Cucumis sativus] Length = 269 Score = 140 bits (353), Expect = 2e-31 Identities = 61/97 (62%), Positives = 76/97 (78%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VNEGSTD+W S+L+E+EDGDGD+GS+QIR+A S EWM M +WGA W + GGP K Sbjct: 172 IAFHVNEGSTDHWLSLLVEFEDGDGDLGSMQIRQANSKEWMEMAHVWGATWCINGGPLKG 231 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S++VTTLST +T+S R+VIP NW P A Y S LNF Sbjct: 232 PFSVKVTTLSTAKTLSARDVIPRNWSPKATYTSRLNF 268 >ref|NP_567803.1| expansin B3 [Arabidopsis thaliana] gi|20138391|sp|Q9M0I2.2|EXPB3_ARATH RecName: Full=Expansin-B3; Short=At-EXPB3; Short=AtEXPB3; AltName: Full=Ath-ExpBeta-1.6; AltName: Full=Beta-expansin-3; Flags: Precursor gi|14532724|gb|AAK64163.1| putative beta-expansin/allergen protein [Arabidopsis thaliana] gi|22136782|gb|AAM91735.1| putative beta-expansin/allergen protein [Arabidopsis thaliana] gi|195604892|gb|ACG24276.1| beta-expansin 3 precursor [Zea mays] gi|332660058|gb|AEE85458.1| expansin B3 [Arabidopsis thaliana] Length = 264 Score = 140 bits (353), Expect = 2e-31 Identities = 57/97 (58%), Positives = 77/97 (79%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VNEGSTD+W S+L+E+EDG+GD+GS+ IR+AG+ EW+ MK +WGANW +IGGP K Sbjct: 164 IAFHVNEGSTDFWLSLLVEFEDGEGDIGSMHIRQAGAREWLEMKHVWGANWCIIGGPLKG 223 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++TTLS +T+S +V+P NW P A Y S LNF Sbjct: 224 PFSIKLTTLSAGKTLSATDVVPRNWAPKATYSSRLNF 260 >ref|NP_001078454.1| expansin B3 [Arabidopsis thaliana] gi|332660059|gb|AEE85459.1| expansin B3 [Arabidopsis thaliana] Length = 258 Score = 140 bits (353), Expect = 2e-31 Identities = 57/97 (58%), Positives = 77/97 (79%) Frame = +1 Query: 1 ITFKVNEGSTDYWFSVLIEYEDGDGDVGSVQIREAGSSEWMSMKQLWGANWALIGGPFKA 180 I F VNEGSTD+W S+L+E+EDG+GD+GS+ IR+AG+ EW+ MK +WGANW +IGGP K Sbjct: 158 IAFHVNEGSTDFWLSLLVEFEDGEGDIGSMHIRQAGAREWLEMKHVWGANWCIIGGPLKG 217 Query: 181 PYSLRVTTLSTNETVSVRNVIPANWQPAAVYKSVLNF 291 P+S+++TTLS +T+S +V+P NW P A Y S LNF Sbjct: 218 PFSIKLTTLSAGKTLSATDVVPRNWAPKATYSSRLNF 254