BLASTX nr result
ID: Ephedra25_contig00007769
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ephedra25_contig00007769
(566 letters)
Database: ./nr
37,332,560 sequences; 13,225,080,153 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AHF65153.1| stylopine synthase, partial [synthetic construct] 123 3e-26
gb|ADB89214.1| stylopine synthase [Papaver somniferum] 123 3e-26
sp|B5UAQ8.1|C7195_ESCCA RecName: Full=Cheilanthifoline synthase;... 122 6e-26
sp|Q50EK4.1|C75A1_PINTA RecName: Full=Cytochrome P450 750A1; Alt... 119 4e-25
gb|ADB89213.1| cheilanthifoline synthase [Papaver somniferum] 118 9e-25
gb|ACO90234.1| stylopine synthase-like protein [Papaver bracteatum] 118 9e-25
gb|ACN40859.1| unknown [Picea sitchensis] 118 1e-24
ref|XP_002991928.1| hypothetical protein SELMODRAFT_134412 [Sela... 117 2e-24
ref|XP_002979995.1| hypothetical protein SELMODRAFT_112052 [Sela... 117 3e-24
gb|AFI57927.1| cytochrome P450, partial [Papaver somniferum] 116 4e-24
sp|B1NF20.1|C719E_ARGME RecName: Full=Cheilanthifoline synthase;... 115 6e-24
gb|AFB74615.1| cytochrome P450 [Papaver somniferum] 115 8e-24
sp|B1NF18.1|C719B_PAPSO RecName: Full=Salutaridine synthase; Alt... 115 8e-24
sp|B1NF19.1|C719D_ARGME RecName: Full=(S)-stylopine synthase; Sh... 115 1e-23
ref|NP_001062194.1| Os08g0508000 [Oryza sativa Japonica Group] g... 114 1e-23
gb|EEE68968.1| hypothetical protein OsJ_27877 [Oryza sativa Japo... 114 1e-23
ref|XP_004487888.1| PREDICTED: isoflavone 2'-hydroxylase-like [C... 113 3e-23
ref|XP_004487887.1| PREDICTED: isoflavone 2'-hydroxylase-like [C... 113 3e-23
ref|NP_001062191.1| Os08g0507100 [Oryza sativa Japonica Group] g... 113 4e-23
gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceus] 113 4e-23
>gb|AHF65153.1| stylopine synthase, partial [synthetic construct]
Length = 511
Score = 123 bits (309), Expect = 3e-26
Identities = 76/195 (38%), Positives = 114/195 (58%), Gaps = 7/195 (3%)
Frame = +3
Query: 3 YFVAHPEMQNKASNEIQTVTPRK--KDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHR 176
+ + P +Q+K E++ +T + + V +ED++KL YL+ VLKE +RM IAPLAIPH+
Sbjct: 324 FLIREPVVQDKLYQELKNLTAKNDCEIVKVEDLNKLQYLQAVLKETMRMKPIAPLAIPHK 383
Query: 177 STRKTILGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMK---MAESKGKE 347
+ R T L G + T V+ NI ++ H+ K+W E KF +RF++ + KG E
Sbjct: 384 ACRDTSLKGNKIDQGTQVMVNIYALHHNEKVWKEP----FKFMPERFLQTQDVVNGKGME 439
Query: 348 RGTNPMCENISFGAGRRQCAGMELANMLVPLTVANLLKAFEWTCETSP--PDLTPYCPFM 521
+ P F AG R CAGMEL + ++ANL+ AFEW+C + PD++ F+
Sbjct: 440 QSLLP------FSAGMRICAGMELGKLQFSFSLANLVNAFEWSCVSDGVLPDMSDQLGFV 493
Query: 522 LRSLKKPLRARIVHR 566
L +K PL+ARIV R
Sbjct: 494 L-LMKTPLQARIVPR 507
>gb|ADB89214.1| stylopine synthase [Papaver somniferum]
Length = 494
Score = 123 bits (309), Expect = 3e-26
Identities = 76/195 (38%), Positives = 114/195 (58%), Gaps = 7/195 (3%)
Frame = +3
Query: 3 YFVAHPEMQNKASNEIQTVTPRK--KDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHR 176
+ + P +Q+K E++ +T + + V +ED++KL YL+ VLKE +RM IAPLAIPH+
Sbjct: 310 FLIREPVVQDKLYQELKNLTAKNDCEIVKVEDLNKLQYLQAVLKETMRMKPIAPLAIPHK 369
Query: 177 STRKTILGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMK---MAESKGKE 347
+ R T L G + T V+ NI ++ H+ K+W E KF +RF++ + KG E
Sbjct: 370 ACRDTSLKGNKIDQGTQVMVNIYALHHNEKVWKEP----FKFMPERFLQTQDVVNGKGME 425
Query: 348 RGTNPMCENISFGAGRRQCAGMELANMLVPLTVANLLKAFEWTCETSP--PDLTPYCPFM 521
+ P F AG R CAGMEL + ++ANL+ AFEW+C + PD++ F+
Sbjct: 426 QSLLP------FSAGMRICAGMELGKLQFSFSLANLVNAFEWSCVSDGVLPDMSDQLGFV 479
Query: 522 LRSLKKPLRARIVHR 566
L +K PL+ARIV R
Sbjct: 480 L-LMKTPLQARIVPR 493
>sp|B5UAQ8.1|C7195_ESCCA RecName: Full=Cheilanthifoline synthase; Short=CHS; AltName:
Full=Cytochrome P450 719A5 gi|209571368|dbj|BAG75113.1|
cheilanthifoline synthase [Eschscholzia californica]
Length = 490
Score = 122 bits (306), Expect = 6e-26
Identities = 77/192 (40%), Positives = 114/192 (59%), Gaps = 4/192 (2%)
Frame = +3
Query: 3 YFVAHPEMQNKASNEIQTVTPRKKDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHRST 182
+ V P +Q K EI +T ++ V +EDVSKL YL+ V+KE +RM IAP+AIPH+++
Sbjct: 304 FLVREPRVQEKLYKEIIDLTGGERSVKVEDVSKLPYLQAVMKETMRMKPIAPMAIPHKTS 363
Query: 183 RKTILGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKMAESKGKERGTNP 362
R T L G V T+++ N+ ++ H+PK++ E KF +RF++ ESK G
Sbjct: 364 RDTSLMGKKVNKGTSIMVNLYAIHHNPKVFPEPY----KFIPERFLQGQESK---YGDIK 416
Query: 363 MCEN--ISFGAGRRQCAGMELANMLVPLTVANLLKAFEWTC--ETSPPDLTPYCPFMLRS 530
E + F AG R CAGMEL + ++A+L++AF+WTC + PDL+ F+L
Sbjct: 417 EMEQSLLPFSAGMRICAGMELGKLQYGFSLASLVEAFKWTCAVDGKLPDLSEDHCFIL-L 475
Query: 531 LKKPLRARIVHR 566
+K PL ARI R
Sbjct: 476 MKNPLEARITPR 487
>sp|Q50EK4.1|C75A1_PINTA RecName: Full=Cytochrome P450 750A1; AltName: Full=Cytochrome P450
CYPC gi|59800268|gb|AAX07433.1| cytochrome P450 CYPC
[Pinus taeda]
Length = 525
Score = 119 bits (299), Expect = 4e-25
Identities = 60/162 (37%), Positives = 88/162 (54%)
Frame = +3
Query: 15 HPEMQNKASNEIQTVTPRKKDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHRSTRKTI 194
HP K EI++V ++ V D++ + YL V+KE LR++ PLA+PH S
Sbjct: 339 HPHAMKKLQQEIESVVGQQGTVKESDLASIVYLHCVVKETLRLYPSLPLALPHESLEAVT 398
Query: 195 LGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKMAESKGKERGTNPMCEN 374
+GG+ +P T V+ N+ ++ DP +WG D E FK +RFM+M E+ G
Sbjct: 399 VGGYYIPKKTMVIMNLWAIGRDPSVWGADASE---FKPERFMQMEENGIDLSGGQSDFRM 455
Query: 375 ISFGAGRRQCAGMELANMLVPLTVANLLKAFEWTCETSPPDL 500
+ FGAGRR C G +A + V T+A LL F+W E P +L
Sbjct: 456 LPFGAGRRTCPGSAMAILTVEFTLAQLLHTFDWRVEGDPSEL 497
>gb|ADB89213.1| cheilanthifoline synthase [Papaver somniferum]
Length = 492
Score = 118 bits (296), Expect = 9e-25
Identities = 72/191 (37%), Positives = 109/191 (57%), Gaps = 3/191 (1%)
Frame = +3
Query: 3 YFVAHPEMQNKASNEIQTVTPRKKDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHRST 182
+ V ++Q K EI VT KK V +ED++KL YL+ V+KE +RM IAP+AIPH+++
Sbjct: 305 FLVREQKIQEKLYREINNVTGGKKPVKVEDLNKLPYLQAVMKETMRMKPIAPMAIPHKAS 364
Query: 183 RKTILGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKMAESKGKERGTNP 362
+ T L G + V+ N+ ++ H+P ++ E KF +RF+K A S G
Sbjct: 365 KDTSLMGKKINKGAVVMVNLYAIHHNPAVFPEPY----KFMPERFLKDANSDGSLGDIKK 420
Query: 363 MCEN-ISFGAGRRQCAGMELANMLVPLTVANLLKAFEWTC--ETSPPDLTPYCPFMLRSL 533
M + ++F AG R CAGMEL + + +A+L+ F+W C E PDL+ F+L +
Sbjct: 421 MESSLLAFSAGMRICAGMELGKLQLAFGLASLVNEFKWDCFAEGKLPDLSEEHCFIL-LM 479
Query: 534 KKPLRARIVHR 566
K PL A+I R
Sbjct: 480 KNPLEAKITPR 490
>gb|ACO90234.1| stylopine synthase-like protein [Papaver bracteatum]
Length = 198
Score = 118 bits (296), Expect = 9e-25
Identities = 76/195 (38%), Positives = 111/195 (56%), Gaps = 7/195 (3%)
Frame = +3
Query: 3 YFVAHPEMQNKASNEIQT--VTPRKKDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHR 176
+ + P +Q K EI+ V ++ V +ED++KL YL+ VLKE +RM IAPLAIPH+
Sbjct: 14 FLIREPVVQEKLYQEIKDFRVKNDREIVKVEDLNKLQYLQAVLKETMRMKPIAPLAIPHK 73
Query: 177 STRKTILGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMK---MAESKGKE 347
+ R T L G + T V+ NI ++ H+ K+W E KF +RF++ + K E
Sbjct: 74 ACRDTSLMGKKIDQGTRVMVNIYALHHNEKVWKEP----FKFMPERFLQTQDVVNGKAME 129
Query: 348 RGTNPMCENISFGAGRRQCAGMELANMLVPLTVANLLKAFEWTCETSP--PDLTPYCPFM 521
+ P F AG R CAGMEL + ++ANL+ AFEW+C + PD++ F+
Sbjct: 130 QSLLP------FSAGMRICAGMELGKLQFSFSLANLVNAFEWSCVSDGVLPDMSDQLGFV 183
Query: 522 LRSLKKPLRARIVHR 566
L +K PL+ARIV R
Sbjct: 184 L-LMKTPLQARIVPR 197
>gb|ACN40859.1| unknown [Picea sitchensis]
Length = 542
Score = 118 bits (295), Expect = 1e-24
Identities = 67/190 (35%), Positives = 95/190 (50%), Gaps = 13/190 (6%)
Frame = +3
Query: 15 HPEMQNKASNEIQTVTPRKKDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHRSTRKTI 194
HP K EI++V + V D+ + YL+ V+KE LR++ PLA+PH S
Sbjct: 356 HPHAMKKLQEEIESVVGQHGIVNESDLGSMVYLQCVVKETLRLYPSLPLALPHASVEAVT 415
Query: 195 LGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKMAESKGKERGTNPMCEN 374
+GG+ +P T V+ N+ ++ DP +WG D E FK +RFM++ E G
Sbjct: 416 VGGYYIPKKTMVILNVWALGRDPMVWGADASE---FKPERFMQVEEHGTDLSGGQSDFRM 472
Query: 375 ISFGAGRRQCAGMELANMLVPLTVANLLKAFEW-------------TCETSPPDLTPYCP 515
+ FGAGRR C G +A + V T+A LL F+W C T+ P TP
Sbjct: 473 LPFGAGRRSCPGSAMAILTVQFTLAQLLNTFDWRVEGDPSELDMKEACATTMPRQTPL-- 530
Query: 516 FMLRSLKKPL 545
F SLK+PL
Sbjct: 531 FAYPSLKRPL 540
>ref|XP_002991928.1| hypothetical protein SELMODRAFT_134412 [Selaginella moellendorffii]
gi|300140314|gb|EFJ07039.1| hypothetical protein
SELMODRAFT_134412 [Selaginella moellendorffii]
Length = 508
Score = 117 bits (293), Expect = 2e-24
Identities = 66/156 (42%), Positives = 89/156 (57%), Gaps = 1/156 (0%)
Frame = +3
Query: 9 VAHPEMQNKASNEIQTVTPRKKDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHRSTRK 188
V +PE+ KA E+ V R + V D SKLTYLE V+KE LR+H P+ +PH S +
Sbjct: 320 VHNPEVMKKAQEELDVVVGRNRMVTETDFSKLTYLEAVIKETLRLHPPVPILVPHMSNKA 379
Query: 189 TILGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKM-AESKGKERGTNPM 365
+L GF VP T + N S++ DP +W EH KF +RF ++ A+ KG++
Sbjct: 380 CVLAGFDVPKGATTIINFYSISRDPNVW---EHP-TKFWPERFGQITADVKGQD------ 429
Query: 366 CENISFGAGRRQCAGMELANMLVPLTVANLLKAFEW 473
E I FGAGRR C GM L V L ++NLL +F W
Sbjct: 430 FELIPFGAGRRMCPGMSLGLKTVHLVLSNLLHSFHW 465
>ref|XP_002979995.1| hypothetical protein SELMODRAFT_112052 [Selaginella moellendorffii]
gi|300152222|gb|EFJ18865.1| hypothetical protein
SELMODRAFT_112052 [Selaginella moellendorffii]
Length = 353
Score = 117 bits (292), Expect = 3e-24
Identities = 66/156 (42%), Positives = 88/156 (56%), Gaps = 1/156 (0%)
Frame = +3
Query: 9 VAHPEMQNKASNEIQTVTPRKKDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHRSTRK 188
V +PE+ KA E+ V R + D SKLTYLE V+KE LR+H P+ +PH S R
Sbjct: 165 VNNPEVMKKAQEELDVVVGRNRMATETDFSKLTYLEAVIKETLRLHPPVPILVPHMSNRA 224
Query: 189 TILGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKM-AESKGKERGTNPM 365
+L GF VP T + N S++ DP +W EH KF +RF ++ A+ KG++
Sbjct: 225 CVLAGFDVPKGATTIINFYSISRDPNVW---EHP-TKFWPERFGQITADVKGQD------ 274
Query: 366 CENISFGAGRRQCAGMELANMLVPLTVANLLKAFEW 473
E I FGAGRR C GM L V L ++NLL +F W
Sbjct: 275 FELIPFGAGRRMCPGMSLGLKTVHLVLSNLLHSFHW 310
>gb|AFI57927.1| cytochrome P450, partial [Papaver somniferum]
Length = 488
Score = 116 bits (290), Expect = 4e-24
Identities = 73/192 (38%), Positives = 105/192 (54%), Gaps = 4/192 (2%)
Frame = +3
Query: 3 YFVAHPEMQNKASNEIQTVTPRKKDVAM--EDVSKLTYLEGVLKEVLRMHAIAPLAIPHR 176
+ + P +Q K E++ T + EDV+KL YL+ V+KE +RM IAPLAIPH+
Sbjct: 308 FLIREPSVQEKLYQELKNFTANNNRTMLKVEDVNKLPYLQAVVKETMRMKPIAPLAIPHK 367
Query: 177 STRKTILGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKMAESKGKERGT 356
+ + T L G V T V+ NI ++ H K+W E KF +RF++ K E+
Sbjct: 368 ACKDTSLMGKKVDKGTKVMVNIHALHHTEKVWKEPY----KFMPERFLQ-KHDKAMEQSL 422
Query: 357 NPMCENISFGAGRRQCAGMELANMLVPLTVANLLKAFEWTCETSP--PDLTPYCPFMLRS 530
P F AG R CAGMEL + ++ANL+ AF+W+C + PD++ F+L
Sbjct: 423 LP------FSAGMRICAGMELGKLQFSFSLANLVNAFKWSCVSDGVLPDMSDLLGFVL-F 475
Query: 531 LKKPLRARIVHR 566
+K PL ARIV R
Sbjct: 476 MKTPLEARIVPR 487
>sp|B1NF20.1|C719E_ARGME RecName: Full=Cheilanthifoline synthase; Short=CHS; AltName:
Full=Cytochrome P450 719A14 gi|148841094|gb|ABR14722.1|
cheilanthifoline synthase [Argemone mexicana]
Length = 494
Score = 115 bits (289), Expect = 6e-24
Identities = 69/191 (36%), Positives = 109/191 (57%), Gaps = 3/191 (1%)
Frame = +3
Query: 3 YFVAHPEMQNKASNEIQTVTPRKKDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHRST 182
+ V E+Q K EI VT K+ V +EDV+K+ YL+ V+KE +RM IAP+AIPH+++
Sbjct: 307 FLVREQEIQEKLYREIVNVTGGKRSVKVEDVNKMPYLQAVMKETMRMKPIAPMAIPHKTS 366
Query: 183 RKTILGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKMAESKGKERGTNP 362
+ T L G + + ++ N+ ++ H+PK++ E KF +RF+K S
Sbjct: 367 KDTSLMGKKINKGSVIMVNLYAIHHNPKVFPEPY----KFMPERFLKDVNSDESLGNIKT 422
Query: 363 MCEN-ISFGAGRRQCAGMELANMLVPLTVANLLKAFEWTCETSP--PDLTPYCPFMLRSL 533
M + ++F AG R CAGMEL + + +A+L+ F+W+C PDL+ F+L +
Sbjct: 423 MESSLLAFSAGMRICAGMELGKLQLAFGLASLVHEFKWSCSVDGKLPDLSEDHCFIL-LM 481
Query: 534 KKPLRARIVHR 566
K PL A+I R
Sbjct: 482 KNPLEAKITCR 492
>gb|AFB74615.1| cytochrome P450 [Papaver somniferum]
Length = 486
Score = 115 bits (288), Expect = 8e-24
Identities = 73/192 (38%), Positives = 105/192 (54%), Gaps = 4/192 (2%)
Frame = +3
Query: 3 YFVAHPEMQNKASNEIQTVTPRKKDVAM--EDVSKLTYLEGVLKEVLRMHAIAPLAIPHR 176
+ + P +Q K E++ T + EDV+KL YL+ V+KE +RM IAPLAIPH+
Sbjct: 306 FLIREPSVQEKLYQELKNFTANNNRTMLKVEDVNKLPYLQAVVKETMRMKPIAPLAIPHK 365
Query: 177 STRKTILGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKMAESKGKERGT 356
+ + T L G V T V+ NI ++ H K+W E KF +RF++ K E+
Sbjct: 366 ACKDTSLMGKKVDKGTKVMVNIHALHHTEKVWKEPY----KFIPERFLQ-KHDKAMEQSL 420
Query: 357 NPMCENISFGAGRRQCAGMELANMLVPLTVANLLKAFEWTCETSP--PDLTPYCPFMLRS 530
P F AG R CAGMEL + ++ANL+ AF+W+C + PD++ F+L
Sbjct: 421 LP------FSAGMRICAGMELGKLQFSFSLANLVNAFKWSCVSDGVLPDMSDLLGFVL-F 473
Query: 531 LKKPLRARIVHR 566
+K PL ARIV R
Sbjct: 474 MKTPLEARIVPR 485
>sp|B1NF18.1|C719B_PAPSO RecName: Full=Salutaridine synthase; AltName: Full=Cytochrome P450
719B1 gi|148841090|gb|ABR14720.1| salutaridine synthase
[Papaver somniferum]
Length = 505
Score = 115 bits (288), Expect = 8e-24
Identities = 70/191 (36%), Positives = 104/191 (54%), Gaps = 3/191 (1%)
Frame = +3
Query: 3 YFVAHPEMQNKASNEIQTVTPRKKDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHRST 182
+ V E+Q K EI T ++ V + D+ +L YL+ V+KE LRM IAPLA+PH +
Sbjct: 314 FLVGEQEIQEKLYREINNRTGGQRPVKVVDLKELPYLQAVMKETLRMKPIAPLAVPHVAA 373
Query: 183 RKTILGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKMAESKGKERGTNP 362
+ T G + T V+ N+ ++ HDP ++ KF +RF+K S G+ N
Sbjct: 374 KDTTFKGRRIVKGTKVMVNLYAIHHDPNVFPAPY----KFMPERFLKDVNSDGRFGDINT 429
Query: 363 MCEN-ISFGAGRRQCAGMELANMLVPLTVANLLKAFEWTC--ETSPPDLTPYCPFMLRSL 533
M + I FGAG R C G+ELA +V +A+++ F+W C E PDL+ F+L +
Sbjct: 430 MESSLIPFGAGMRICGGVELAKQMVAFALASMVNEFKWDCVSEGKLPDLSEAISFIL-YM 488
Query: 534 KKPLRARIVHR 566
K PL A+I R
Sbjct: 489 KNPLEAKITPR 499
>sp|B1NF19.1|C719D_ARGME RecName: Full=(S)-stylopine synthase; Short=STS; AltName:
Full=Cytochrome P450 719A13 gi|148841092|gb|ABR14721.1|
stylopine synthase [Argemone mexicana]
Length = 504
Score = 115 bits (287), Expect = 1e-23
Identities = 74/196 (37%), Positives = 107/196 (54%), Gaps = 8/196 (4%)
Frame = +3
Query: 3 YFVAHPEMQNKASNEIQTVTPR--KKDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHR 176
Y + P +Q K E++ T +K V ED++KL YL+ V+KE +RM IAPLAIPH+
Sbjct: 319 YLIREPNVQEKLYEELKNFTNDNDRKMVKFEDLNKLQYLQAVVKETMRMKPIAPLAIPHK 378
Query: 177 STRKTILGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKM----AESKGK 344
+ R+T L G V T V+ NI ++ H+ +W E KF +RF++ + K
Sbjct: 379 ACRETSLMGRKVNQGTRVMVNIYALHHNQNVWKEPY----KFNPERFLQKNQDGVDGKAM 434
Query: 345 ERGTNPMCENISFGAGRRQCAGMELANMLVPLTVANLLKAFEWTCETSP--PDLTPYCPF 518
E+ P F AG R CAGMEL + +ANL+ AF+W+C + PD++ F
Sbjct: 435 EQSLLP------FSAGMRICAGMELGKLQFSFALANLVNAFKWSCVSDGVFPDMSDQLGF 488
Query: 519 MLRSLKKPLRARIVHR 566
+L +K PL A IV R
Sbjct: 489 VL-LMKTPLEAGIVPR 503
>ref|NP_001062194.1| Os08g0508000 [Oryza sativa Japonica Group]
gi|19849281|gb|AAL99547.1|AF488522_1 Cyt-P450
monooxygenase [Oryza sativa Japonica Group]
gi|42408984|dbj|BAD10239.1| Cyt-P450 monooxygenase
[Oryza sativa Japonica Group]
gi|42409340|dbj|BAD10655.1| Cyt-P450 monooxygenase
[Oryza sativa Japonica Group]
gi|113624163|dbj|BAF24108.1| Os08g0508000 [Oryza sativa
Japonica Group] gi|125544489|gb|EAY90628.1| hypothetical
protein OsI_12230 [Oryza sativa Indica Group]
gi|215716994|dbj|BAG95357.1| unnamed protein product
[Oryza sativa Japonica Group]
Length = 506
Score = 114 bits (286), Expect = 1e-23
Identities = 59/156 (37%), Positives = 92/156 (58%), Gaps = 3/156 (1%)
Frame = +3
Query: 15 HPEMQNKASNEIQTVTPRKKDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHRSTRKTI 194
+P + KA E++ V KK + D KL YL V+KE +R+H +AP+ +PH++ +
Sbjct: 318 NPSIMAKARTEMEDVLAGKKTIEENDTEKLPYLRAVIKEAMRLHPVAPILLPHQAAEDGV 377
Query: 195 -LGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKMAE--SKGKERGTNPM 365
+GG+ VP +TV+FN+ ++ DP W D+F +RF++ AE +GK+
Sbjct: 378 EIGGYAVPKGSTVIFNVWAIMRDPTAW----ERPDEFMPERFLQRAEVDFRGKD------ 427
Query: 366 CENISFGAGRRQCAGMELANMLVPLTVANLLKAFEW 473
E + FGAGRR C G+ +A +VP +A+LL AFEW
Sbjct: 428 FEFMPFGAGRRLCPGLPMAERVVPFILASLLHAFEW 463
>gb|EEE68968.1| hypothetical protein OsJ_27877 [Oryza sativa Japonica Group]
Length = 481
Score = 114 bits (286), Expect = 1e-23
Identities = 59/156 (37%), Positives = 92/156 (58%), Gaps = 3/156 (1%)
Frame = +3
Query: 15 HPEMQNKASNEIQTVTPRKKDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHRSTRKTI 194
+P + KA E++ V KK + D KL YL V+KE +R+H +AP+ +PH++ +
Sbjct: 293 NPSIMAKARTEMEDVLAGKKTIEENDTEKLPYLRAVIKEAMRLHPVAPILLPHQAAEDGV 352
Query: 195 -LGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKMAE--SKGKERGTNPM 365
+GG+ VP +TV+FN+ ++ DP W D+F +RF++ AE +GK+
Sbjct: 353 EIGGYAVPKGSTVIFNVWAIMRDPTAW----ERPDEFMPERFLQRAEVDFRGKD------ 402
Query: 366 CENISFGAGRRQCAGMELANMLVPLTVANLLKAFEW 473
E + FGAGRR C G+ +A +VP +A+LL AFEW
Sbjct: 403 FEFMPFGAGRRLCPGLPMAERVVPFILASLLHAFEW 438
>ref|XP_004487888.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cicer arietinum]
Length = 500
Score = 113 bits (283), Expect = 3e-23
Identities = 62/157 (39%), Positives = 91/157 (57%)
Frame = +3
Query: 15 HPEMQNKASNEIQTVTPRKKDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHRSTRKTI 194
HP++ NKA EI+T + K + D+SKL YL+ ++ E LR+H APL++PH S+
Sbjct: 323 HPDVLNKAKEEIETQIGKDKLIEEHDLSKLPYLQNIINETLRLHPPAPLSLPHYSSEDFT 382
Query: 195 LGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKMAESKGKERGTNPMCEN 374
LGGF VP +T ++ N+ ++ DPK W + FK +RF E G+ + +
Sbjct: 383 LGGFNVPKDTIILTNVWAIHRDPKYWS----DASSFKPERF----EIDGE------LNKL 428
Query: 375 ISFGAGRRQCAGMELANMLVPLTVANLLKAFEWTCET 485
I+FG GRR C G LA+ V LT+ L++ FEW ET
Sbjct: 429 IAFGLGRRACPGQSLAHRTVGLTLGLLIQCFEWKRET 465
>ref|XP_004487887.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cicer arietinum]
Length = 500
Score = 113 bits (283), Expect = 3e-23
Identities = 63/157 (40%), Positives = 91/157 (57%)
Frame = +3
Query: 15 HPEMQNKASNEIQTVTPRKKDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHRSTRKTI 194
HP++ NKA EI+T R K + D+SKL YL+ ++ E LR+H APL +PH S+
Sbjct: 323 HPDVLNKAKEEIETQIGRDKLIEEHDLSKLPYLQNIINETLRLHPPAPLLLPHYSSEDFT 382
Query: 195 LGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKMAESKGKERGTNPMCEN 374
LGGF VP +T ++ N+ ++ DP LW + FK +RF E +G+ + +
Sbjct: 383 LGGFNVPKDTIILSNVWAIHRDPNLWSDPL----SFKPERF----EIEGE------LNKL 428
Query: 375 ISFGAGRRQCAGMELANMLVPLTVANLLKAFEWTCET 485
I+FG GRR C G+ LA V LTV L++ FEW E+
Sbjct: 429 IAFGLGRRACPGLNLAQRSVGLTVGMLIQCFEWKRES 465
>ref|NP_001062191.1| Os08g0507100 [Oryza sativa Japonica Group]
gi|19849279|gb|AAL99546.1|AF488521_1 Cyt-P450
monooxygenase [Oryza sativa Japonica Group]
gi|42408935|dbj|BAD10192.1| Cyt-P450 monooxygenase
[Oryza sativa Japonica Group]
gi|42408977|dbj|BAD10232.1| Cyt-P450 monooxygenase
[Oryza sativa Japonica Group]
gi|113624160|dbj|BAF24105.1| Os08g0507100 [Oryza sativa
Japonica Group] gi|125603952|gb|EAZ43277.1| hypothetical
protein OsJ_27874 [Oryza sativa Japonica Group]
Length = 505
Score = 113 bits (282), Expect = 4e-23
Identities = 59/156 (37%), Positives = 90/156 (57%), Gaps = 3/156 (1%)
Frame = +3
Query: 15 HPEMQNKASNEIQTVTPRKKDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHRSTRKTI 194
+P + K E++ V KK + D KL YL V+KE +R+H +AP+ +PHR+ +
Sbjct: 322 NPSIMAKVRAEMEDVLAGKKTIEENDTEKLPYLRAVIKEAMRLHPVAPILLPHRAAEDGV 381
Query: 195 -LGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKMAE--SKGKERGTNPM 365
+GG+ VP +TV+FN+ ++ DP W E +F +RF++ AE +GK+
Sbjct: 382 EIGGYAVPKGSTVIFNVWTIMRDPAAWERPE----EFMPERFLQRAEVDFRGKD------ 431
Query: 366 CENISFGAGRRQCAGMELANMLVPLTVANLLKAFEW 473
E I FGAGRR C G+ + +VP +A+LL AFEW
Sbjct: 432 FEFIPFGAGRRLCPGLPMTERVVPFILASLLHAFEW 467
>gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceus]
Length = 505
Score = 113 bits (282), Expect = 4e-23
Identities = 66/184 (35%), Positives = 100/184 (54%), Gaps = 7/184 (3%)
Frame = +3
Query: 15 HPEMQNKASNEIQTVTPRKKDVAMEDVSKLTYLEGVLKEVLRMHAIAPLAIPHRSTRKTI 194
+PE+ KA EI+T +++ V +D+S+L YL+ V+ E LR+H APL +PH ++
Sbjct: 328 NPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRLHPPAPLLLPHSASEDCT 387
Query: 195 LGGFVVPANTTVVFNIDSVAHDPKLWGEDEHELDKFKAQRFMKMAESKGKERGTNPMCEN 374
+GGF VP +T ++ NI ++ DP+LW + FK +RF K E + +
Sbjct: 388 IGGFNVPKDTIILTNIWAIHRDPELWTDP----SSFKPERFEKEGE----------VNKL 433
Query: 375 ISFGAGRRQCAGMELANMLVPLTVANLLKAFEWTCETSP-------PDLTPYCPFMLRSL 533
+SFG GRR C G+ LA V TV L++ FEW E+ +T F LR++
Sbjct: 434 LSFGLGRRACPGLSLAQRTVGYTVGLLIQGFEWKTESEEKLDLAEGKGITMPMKFPLRAM 493
Query: 534 KKPL 545
KPL
Sbjct: 494 CKPL 497