BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00033726
         (752 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAN67321.1| hypothetical protein VITISV_039348 [Vitis vinifera]   215   7e-54
ref|XP_002278453.1| PREDICTED: uncharacterized mscS family prote...   214   1e-53
ref|XP_004170243.1| PREDICTED: mechanosensitive ion channel prot...   210   3e-52
ref|XP_004140046.1| PREDICTED: mechanosensitive ion channel prot...   210   3e-52
ref|NP_177982.1| mechanosensitive channel of small conductance-l...   204   1e-50

>emb|CAN67321.1| hypothetical protein VITISV_039348 [Vitis vinifera]
          Length = 922

 Score =  215 bits (548), Expect = 7e-54
 Identities = 128/272 (47%), Positives = 160/272 (58%), Gaps = 23/272 (8%)
 Frame = -2

Query: 748 FIRKQRASGEELSLDMDFEMEELKRESSKD---------RRVXXXXXXXXXXXXXXEDAV 596
           F+ KQ+ASG+  SLDMD EMEEL+ E S+                            D +
Sbjct: 140 FLHKQKASGD-FSLDMDMEMEELRDEKSEPPMPESNMHPMMSSREMKVSFQPQTTGADEM 198

Query: 595 RSGSVHR----RGGARVPTSPRDD--------GGGEVLRCTSNSSFRRSSTLLRVKTRSR 452
           RS +V R    + G+    S  DD          GEVL CTSN  F+R STLLR KT+SR
Sbjct: 199 RSETVRRSYIDKEGSDKDGSDEDDIKRDNCDNPDGEVLMCTSNMEFQRKSTLLRNKTKSR 258

Query: 451 LMDPAPAAFDDNR--KSGKIPRSGQMKSGMLGKXXXXXXXXXXXXXXXDGFNQDSWGVFM 278
           L D            KSG +P+SG +KSG+LGK               + F + ++  + 
Sbjct: 259 LADRTEYVMKSGLVPKSGLLPKSGMLKSGLLGKSEEDEEDPFFVDDLPEEFKRSNFSFWT 318

Query: 277 ILQLVSFILIIAFFFCSIFIPVLERQTVWNLHLWKWELLVLVLICGRLVSGWVIRLIVFF 98
           ILQ +  IL++A   CS+ IP+ + + +W L LW+WE++VLVLICGRLVSGW IRL+VFF
Sbjct: 319 ILQWLILILLVAVLVCSLTIPLFKDRILWKLRLWRWEVMVLVLICGRLVSGWGIRLVVFF 378

Query: 97  IERNFLLRKRVLYFVYGLRKAVQNCLWLGLVL 2
           IERNFLLRKRVLYFVYGLRKAVQNCLWLGLVL
Sbjct: 379 IERNFLLRKRVLYFVYGLRKAVQNCLWLGLVL 410


>ref|XP_002278453.1| PREDICTED: uncharacterized mscS family protein At1g78610-like
           [Vitis vinifera]
          Length = 897

 Score =  214 bits (546), Expect = 1e-53
 Identities = 122/251 (48%), Positives = 154/251 (61%), Gaps = 2/251 (0%)
 Frame = -2

Query: 748 FIRKQRASGEELSLDMDFEMEELKRESSKDRRVXXXXXXXXXXXXXXEDAVRSGSVHRRG 569
           F+ KQ+ASG+  SLDMD EMEEL+ E S+   +              +D      + R  
Sbjct: 140 FLHKQKASGD-FSLDMDMEMEELRDEKSEPP-MPESNMHPMMSSREMKDGSDEDDIKRDN 197

Query: 568 GARVPTSPRDDGGGEVLRCTSNSSFRRSSTLLRVKTRSRLMDPAPAAFDDNR--KSGKIP 395
                    D+  GEVL CTSN  F+R STLLR KT+SRL D            KSG +P
Sbjct: 198 C--------DNPDGEVLMCTSNMEFQRKSTLLRNKTKSRLADRTEYVMKSGLVPKSGLLP 249

Query: 394 RSGQMKSGMLGKXXXXXXXXXXXXXXXDGFNQDSWGVFMILQLVSFILIIAFFFCSIFIP 215
           +SG +KSG+LGK               + F + ++  + ILQ +  IL++A   CS+ IP
Sbjct: 250 KSGMLKSGLLGKSEEDEEDPFFVDDLPEEFKRSNFSFWTILQWLILILLVAVLVCSLTIP 309

Query: 214 VLERQTVWNLHLWKWELLVLVLICGRLVSGWVIRLIVFFIERNFLLRKRVLYFVYGLRKA 35
           + + + +W L LW+WE++VLVLICGRLVSGW IRL+VFFIERNFLLRKRVLYFVYGLRKA
Sbjct: 310 LFKDRILWKLRLWRWEVMVLVLICGRLVSGWGIRLVVFFIERNFLLRKRVLYFVYGLRKA 369

Query: 34  VQNCLWLGLVL 2
           VQNCLWLGLVL
Sbjct: 370 VQNCLWLGLVL 380


>ref|XP_004170243.1| PREDICTED: mechanosensitive ion channel protein 8-like [Cucumis
           sativus]
          Length = 923

 Score =  210 bits (534), Expect = 3e-52
 Identities = 125/279 (44%), Positives = 163/279 (58%), Gaps = 30/279 (10%)
 Frame = -2

Query: 748 FIRKQRASGEELSLDMDFEMEELK--------------RESSKDRRVXXXXXXXXXXXXX 611
           F+ KQ+ASGE +SLDMD EM EL               R SS++ +V             
Sbjct: 127 FLHKQKASGE-MSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEISENDS 185

Query: 610 XEDAVRSGSVHRRGGARVP---------TSPRDDGGGEVLRCTSNSSFRRS------STL 476
                R   +      + P         ++  DDG  EVLRC+SNSSF+R       S+L
Sbjct: 186 MRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSSFQRDVSFQRKSSL 245

Query: 475 LRVKTRSRLMDPAPAAFDDNRKSGKIPRSGQMKSGMLGKXXXXXXXXXXXXXXXDG-FNQ 299
           LR KT+SRL+DP       +R+SG++P+SGQ++SG++ K                  + +
Sbjct: 246 LRAKTKSRLLDPPE---HQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEYKK 302

Query: 298 DSWGVFMILQLVSFILIIAFFFCSIFIPVLERQTVWNLHLWKWELLVLVLICGRLVSGWV 119
            + GV  +LQ  S ILIIA   C++ I    R+ +W L +WKWE+++LVLICGRLVSGW 
Sbjct: 303 ANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWG 362

Query: 118 IRLIVFFIERNFLLRKRVLYFVYGLRKAVQNCLWLGLVL 2
           IR+IVFFIERNFLLRKRVLYFVYG+RKAVQNCLWLGLVL
Sbjct: 363 IRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVL 401


>ref|XP_004140046.1| PREDICTED: mechanosensitive ion channel protein 8-like [Cucumis
           sativus]
          Length = 923

 Score =  210 bits (534), Expect = 3e-52
 Identities = 125/279 (44%), Positives = 163/279 (58%), Gaps = 30/279 (10%)
 Frame = -2

Query: 748 FIRKQRASGEELSLDMDFEMEELK--------------RESSKDRRVXXXXXXXXXXXXX 611
           F+ KQ+ASGE +SLDMD EM EL               R SS++ +V             
Sbjct: 127 FLHKQKASGE-MSLDMDMEMLELPQDKTPLSTVAESPMRRSSRELKVSFESISEISENDS 185

Query: 610 XEDAVRSGSVHRRGGARVP---------TSPRDDGGGEVLRCTSNS------SFRRSSTL 476
                R   +      + P         ++  DDG  EVLRC+SNS      SF+R S+L
Sbjct: 186 MRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSSL 245

Query: 475 LRVKTRSRLMDPAPAAFDDNRKSGKIPRSGQMKSGMLGKXXXXXXXXXXXXXXXDG-FNQ 299
           LR KT+SRL+DP       +R+SG++P+SGQ++SG++ K                  + +
Sbjct: 246 LRAKTKSRLLDPPE---HQDRRSGRVPKSGQVRSGLISKALDEEDDDPFLEEDLPDEYKK 302

Query: 298 DSWGVFMILQLVSFILIIAFFFCSIFIPVLERQTVWNLHLWKWELLVLVLICGRLVSGWV 119
            + GV  +LQ  S ILIIA   C++ I    R+ +W L +WKWE+++LVLICGRLVSGW 
Sbjct: 303 ANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEVMILVLICGRLVSGWG 362

Query: 118 IRLIVFFIERNFLLRKRVLYFVYGLRKAVQNCLWLGLVL 2
           IR+IVFFIERNFLLRKRVLYFVYG+RKAVQNCLWLGLVL
Sbjct: 363 IRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVL 401


>ref|NP_177982.1| mechanosensitive channel of small conductance-like 6 [Arabidopsis
           thaliana] gi|75213461|sp|Q9SYM1.1|MSL6_ARATH RecName:
           Full=Mechanosensitive ion channel protein 6; AltName:
           Full=Mechanosensitive channel of small conductance-like
           6; AltName: Full=MscS-Like protein 6
           gi|4836872|gb|AAD30575.1|AC007260_6 Hypothetical protein
           [Arabidopsis thaliana] gi|332198006|gb|AEE36127.1|
           mechanosensitive channel of small conductance-like 6
           [Arabidopsis thaliana]
          Length = 856

 Score =  204 bits (520), Expect = 1e-50
 Identities = 122/263 (46%), Positives = 163/263 (61%), Gaps = 14/263 (5%)
 Frame = -2

Query: 748 FIRKQRASGEELSLDMDFEMEELKRES----SKDRRVXXXXXXXXXXXXXXEDAVRSGSV 581
           F+ KQ+ASGE + LDMD  M+EL+       S+  RV                + +   V
Sbjct: 92  FLHKQQASGE-ICLDMDLGMDELQSRGLTPVSESPRV----------------STKRDPV 134

Query: 580 HRRGGARVPTSPRDDGGGEVLRCTSNSS--FRRSSTLLRVKTRSRLMDPAPAAFDD---N 416
            RR  +R  T+  DDG  EV++C+ N++   R SSTLL+++TRSRL DP          +
Sbjct: 135 GRRD-SRSNTNNNDDG--EVVKCSGNNAPIQRSSSTLLKMRTRSRLSDPPTPQLPPQTAD 191

Query: 415 RKSGKIPRSGQMKSGMLGKXXXXXXXXXXXXXXXDG-----FNQDSWGVFMILQLVSFIL 251
            KSG+IP+SGQMKSG  GK                      + +D   ++++L+ +S IL
Sbjct: 192 MKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLIL 251

Query: 250 IIAFFFCSIFIPVLERQTVWNLHLWKWELLVLVLICGRLVSGWVIRLIVFFIERNFLLRK 71
           IIA F C++ IP L ++ +W L LWKWE +VLVLICGRLVS W+++++VFFIERNFLLRK
Sbjct: 252 IIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRK 311

Query: 70  RVLYFVYGLRKAVQNCLWLGLVL 2
           RVLYFVYG+RKAVQNCLWLGLVL
Sbjct: 312 RVLYFVYGVRKAVQNCLWLGLVL 334