BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00029474
         (514 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]       162   3e-38
ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [V...   159   3e-37
ref|XP_002524942.1| cytochrome P450, putative [Ricinus communis]...   158   4e-37
ref|XP_002283500.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis ...   157   1e-36
emb|CAA04116.1| cytochrome P450 [Helianthus tuberosus]                157   1e-36

>dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 503

 Score =  162 bits (410), Expect = 3e-38
 Identities = 88/171 (51%), Positives = 113/171 (66%)
 Frame = +2

Query: 2   KTKFFGLTYNIIMRMLANKRYYGDADESSSEAGKEFRELTKETFILSGTSNAADFLPVVR 181
           KT  F LT N++M M+A KRYYG   E   +A K F+E+ + T  +SG SN  DFLP++ 
Sbjct: 168 KTMIFELTLNVMMTMIAGKRYYGGQVEELEQA-KRFQEIVQGTLAISGASNIGDFLPILS 226

Query: 182 WFDIGGHEXXXXXXXXXXNKFFQGLIEEHRMKKESRSQGMDSSPAGRSTVIDLLLSMQER 361
           W  I G E          +KF Q LIEEHR+   SRS GM +     +T+ID+LLS+QE 
Sbjct: 227 WIGIKGIEKKVWNLKEKRDKFLQELIEEHRI---SRSGGMQTKEKN-ATLIDVLLSLQET 282

Query: 362 DPEHYDDDMIKGFIDLMLVAGTDTSASTMEWTMSLLLNNPQTLEKLRAEID 514
           +PE+Y D+MI G I ++L AGTDTSA TMEW MSLLLNNPQ ++K +AEID
Sbjct: 283 EPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMSLLLNNPQVIKKAQAEID 333


>ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 503

 Score =  159 bits (401), Expect = 3e-37
 Identities = 87/172 (50%), Positives = 112/172 (65%), Gaps = 1/172 (0%)
 Frame = +2

Query: 2   KTKFFGLTYNIIMRMLANKRYYGDADESSSEAGKEFRELTKETFILSGTSNAADFLPVVR 181
           K+ FF LT N +MRM++ KRYYGD + +  E  ++FRE+  ETF LSG +N  DF+P  +
Sbjct: 171 KSMFFELTLNNMMRMISGKRYYGD-NVTELEETRKFREIVAETFELSGATNIVDFVPFSK 229

Query: 182 WFDIGGHEXXXXXXXXXXNKFFQGLIEEHRMKKESRSQGMDSSPAGRS-TVIDLLLSMQE 358
           W  + G E          + F Q LIEEHR         M S   GRS T++D+LLS+QE
Sbjct: 230 WIGLNGIEKKLVILQGKRDGFMQNLIEEHRR--------MRSPCEGRSKTMLDVLLSLQE 281

Query: 359 RDPEHYDDDMIKGFIDLMLVAGTDTSASTMEWTMSLLLNNPQTLEKLRAEID 514
            +PE Y DD+I+G + +ML AGTDTSA TMEW MSLLLNNP+ LEK +AEID
Sbjct: 282 TEPECYTDDIIRGMMQVMLSAGTDTSAGTMEWAMSLLLNNPEALEKAQAEID 333


>ref|XP_002524942.1| cytochrome P450, putative [Ricinus communis]
           gi|223535777|gb|EEF37439.1| cytochrome P450, putative
           [Ricinus communis]
          Length = 452

 Score =  158 bits (400), Expect = 4e-37
 Identities = 84/171 (49%), Positives = 113/171 (66%)
 Frame = +2

Query: 2   KTKFFGLTYNIIMRMLANKRYYGDADESSSEAGKEFRELTKETFILSGTSNAADFLPVVR 181
           K+KF  LT++I++RM+A KRYYGD D S  E  ++F +L KE     G +N  DFLP++ 
Sbjct: 122 KSKFQELTFSIMIRMVAGKRYYGD-DVSDKEEARQFTQLMKEAVTYGGATNPGDFLPILN 180

Query: 182 WFDIGGHEXXXXXXXXXXNKFFQGLIEEHRMKKESRSQGMDSSPAGRSTVIDLLLSMQER 361
           W D GG +          +KFFQ LI+EHR KKE+    ++S+    +T+ID LLS+QE 
Sbjct: 181 WIDGGGFKKRIIGLSKRTDKFFQALIDEHRSKKEN----LEST----NTMIDHLLSLQES 232

Query: 362 DPEHYDDDMIKGFIDLMLVAGTDTSASTMEWTMSLLLNNPQTLEKLRAEID 514
           +PE+Y D++IKG    ML AGTDTSA T+EW MS LLN+P TL K + EID
Sbjct: 233 EPEYYTDEIIKGLTQNMLFAGTDTSAITLEWAMSNLLNHPSTLRKAKDEID 283


>ref|XP_002283500.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis vinifera]
          Length = 504

 Score =  157 bits (396), Expect = 1e-36
 Identities = 82/166 (49%), Positives = 111/166 (66%), Gaps = 1/166 (0%)
 Frame = +2

Query: 20  LTYNIIMRMLANKRYYGDADESSSEAGKEFRELTKETFILSG-TSNAADFLPVVRWFDIG 196
           LT+NII RM+A KRYYG+ D   +EA K FRE+  + FIL G +SN ADFLP++RW  +G
Sbjct: 181 LTFNIITRMVAGKRYYGE-DVEYTEA-KRFREIISQLFILGGASSNPADFLPILRWIGLG 238

Query: 197 GHEXXXXXXXXXXNKFFQGLIEEHRMKKESRSQGMDSSPAGRSTVIDLLLSMQERDPEHY 376
            HE              QGLI+EHR        G D      +++ID LLS+Q+ +PE+Y
Sbjct: 239 YHEKKLKKIVRETRAILQGLIDEHR-------SGNDKGSVDNNSMIDHLLSLQKTEPEYY 291

Query: 377 DDDMIKGFIDLMLVAGTDTSASTMEWTMSLLLNNPQTLEKLRAEID 514
            DD+IKG + ++++AGTDTSA+TMEW M+LLLN+P  LEK +AE+D
Sbjct: 292 TDDIIKGLVQVLILAGTDTSAATMEWAMTLLLNHPDVLEKAKAELD 337


>emb|CAA04116.1| cytochrome P450 [Helianthus tuberosus]
          Length = 505

 Score =  157 bits (396), Expect = 1e-36
 Identities = 78/171 (45%), Positives = 110/171 (64%)
 Frame = +2

Query: 2   KTKFFGLTYNIIMRMLANKRYYGDADESSSEAGKEFRELTKETFILSGTSNAADFLPVVR 181
           K  F+ LT N++MRM++ KRY+G  +    E GK FR++  ETF+L+G SN  D+LPV+ 
Sbjct: 171 KFSFYELTLNVMMRMISGKRYFGGDNPELEEEGKRFRDMLDETFVLAGASNVGDYLPVLS 230

Query: 182 WFDIGGHEXXXXXXXXXXNKFFQGLIEEHRMKKESRSQGMDSSPAGRSTVIDLLLSMQER 361
           W  + G E          + FFQGLI++ R  K +           + T+I+LLLS+QE 
Sbjct: 231 WLGVKGLEKKLIKLQEKRDVFFQGLIDQLRKSKGTEDVNK------KKTMIELLLSLQET 284

Query: 362 DPEHYDDDMIKGFIDLMLVAGTDTSASTMEWTMSLLLNNPQTLEKLRAEID 514
           +PE+Y D MI+ F+ ++L AG+DTSA TMEW MSLLLN+PQ L+K + EID
Sbjct: 285 EPEYYTDAMIRSFVLVLLAAGSDTSAGTMEWVMSLLLNHPQVLKKAQNEID 335