BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00025544
         (592 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAG32267.1| gibberellin 3-oxidase [Allium fistulosum]             264   7e-69
dbj|BAD91162.1| gibberellin 3-beta hydroxylase [Prunus subhirtella]   227   1e-57
gb|AFS18537.1| gibberellin 3-oxidase [Pyrus communis]                 220   1e-55
dbj|BAG48321.1| gibberellin 3-oxidase2 [Chrysanthemum x morifolium]   220   2e-55
gb|ACT99991.1| gibberellin 3-oxidase [Malus x domestica]              216   2e-54

>dbj|BAG32267.1| gibberellin 3-oxidase [Allium fistulosum]
          Length = 365

 Score =  264 bits (675), Expect = 7e-69
 Identities = 126/195 (64%), Positives = 160/195 (82%), Gaps = 1/195 (0%)
 Frame = -1

Query: 592 RVMHLMLQSLGLNEDDLDWAGPVKDLQEVSGVLQLNSYPSCPDPDRAMGLAAHTDSSLLT 413
           R+M LML SLGL E+ + WAGP+ +LQ++S VLQLNSYP+CP+PDRA+GLAAHTDSSLLT
Sbjct: 174 RLMQLMLLSLGLTEETIKWAGPMNELQDISSVLQLNSYPACPNPDRAIGLAAHTDSSLLT 233

Query: 412 ILHQNSTSGLQVLRAQDIYGPARWVTVPPLPGTLVINIGDLSHILSNGRFHSVLHRAVVN 233
           IL+Q++TSGLQVLR      P +WVTVPP+PG LV+N+GDL HILSNGRFHSV+HRAVVN
Sbjct: 234 ILYQSNTSGLQVLRPNKESSPTQWVTVPPIPGALVVNVGDLCHILSNGRFHSVVHRAVVN 293

Query: 232 RVCHRYSMAYICGPPAHAKVSPL-RPLSGRPVFRAVTWPEYLGLKGKLFNQALASIRVSD 56
           R  HRYS AYICGPPAH KVSP+ +P+   P +R +TW EYLG+K +LF++ALAS+ +S+
Sbjct: 294 RTEHRYSAAYICGPPAHVKVSPIVKPVG--PAYRPITWREYLGIKARLFDKALASVIISE 351

Query: 55  ETWEDHNNASVITCV 11
           E   D N+  +++CV
Sbjct: 352 EDHND-NSTCLLSCV 365


>dbj|BAD91162.1| gibberellin 3-beta hydroxylase [Prunus subhirtella]
          Length = 372

 Score =  227 bits (579), Expect = 1e-57
 Identities = 118/198 (59%), Positives = 144/198 (72%), Gaps = 8/198 (4%)
 Frame = -1

Query: 592 RVMHLMLQSLGLNEDDLDWAGPVKDLQEVSGVLQLNSYPSCPDPDRAMGLAAHTDSSLLT 413
           R+M LML SLG++ +D+ WAGP  D ++ S  LQLNSYP+CPDPDRAMGLAAHTDS+LLT
Sbjct: 179 RLMWLMLGSLGISMEDVKWAGPKGDFRDASAALQLNSYPACPDPDRAMGLAAHTDSTLLT 238

Query: 412 ILHQNSTSGLQVLRAQDIYGPARWVTVPPLPGTLVINIGDLSHILSNGRFHSVLHRAVVN 233
           IL+QN+TSGLQVLR         WVTVPPLPG LV+N+GDL HILSNG + SVLHRAVVN
Sbjct: 239 ILYQNNTSGLQVLRE-----GTGWVTVPPLPGALVVNVGDLIHILSNGLYPSVLHRAVVN 293

Query: 232 RVCHRYSMAYICGPPAHAKVSPLRPLSG---RPVFRAVTWPEYLGLKGKLFNQALASIRV 62
           R  HR S+AY+ GPPA  ++SPL  L G    P++R +TW EYLG K K FN+AL+S+R+
Sbjct: 294 RSRHRLSIAYLYGPPASVQISPLSKLLGPSQPPLYRPITWNEYLGTKAKHFNKALSSVRL 353

Query: 61  SDE-----TWEDHNNASV 23
                      DHN+  V
Sbjct: 354 CAPLNALVDVSDHNSVKV 371


>gb|AFS18537.1| gibberellin 3-oxidase [Pyrus communis]
          Length = 371

 Score =  220 bits (561), Expect = 1e-55
 Identities = 114/199 (57%), Positives = 143/199 (71%), Gaps = 8/199 (4%)
 Frame = -1

Query: 592 RVMHLMLQSLGLNEDDLDWAGPVKDLQEVSGVLQLNSYPSCPDPDRAMGLAAHTDSSLLT 413
           R+M LML SLG++ +D+ WAG   + +  S  LQLNSYP+CP+PDRAMGLAAHTDS+LLT
Sbjct: 178 RLMWLMLGSLGISPEDVKWAGSKGEFKGASAALQLNSYPACPEPDRAMGLAAHTDSTLLT 237

Query: 412 ILHQNSTSGLQVLRAQDIYGPARWVTVPPLPGTLVINIGDLSHILSNGRFHSVLHRAVVN 233
           IL+QN+TSGLQVLR         WVTVPP+ G LV+N+GDL HILSNG + SVLHRAVVN
Sbjct: 238 ILYQNNTSGLQVLR-----DGTGWVTVPPMAGALVVNVGDLIHILSNGVYPSVLHRAVVN 292

Query: 232 RVCHRYSMAYICGPPAHAKVSPLRPLSG---RPVFRAVTWPEYLGLKGKLFNQALASIRV 62
           R  HR S+AY+ GPPA  ++SPL  L G    P++R +TW EYLG K K FN+AL+S+R+
Sbjct: 293 RTQHRLSIAYLFGPPASVQISPLSKLVGPSHPPLYRPITWNEYLGTKAKHFNKALSSVRL 352

Query: 61  -----SDETWEDHNNASVI 20
                S     DHN+  V+
Sbjct: 353 CAPLSSSADVNDHNSVKVV 371


>dbj|BAG48321.1| gibberellin 3-oxidase2 [Chrysanthemum x morifolium]
          Length = 358

 Score =  220 bits (560), Expect = 2e-55
 Identities = 108/181 (59%), Positives = 137/181 (75%), Gaps = 3/181 (1%)
 Frame = -1

Query: 592 RVMHLMLQSLGLNEDDLDWAGPVKDLQEVSGVLQLNSYPSCPDPDRAMGLAAHTDSSLLT 413
           R+M L+L SLG+ E D+ WAGP  DL+E    LQLNSYP+CPDPDRAMGLAAHTDSSLLT
Sbjct: 165 RLMWLVLGSLGITEGDVKWAGPAGDLKEACPALQLNSYPACPDPDRAMGLAAHTDSSLLT 224

Query: 412 ILHQNSTSGLQVLRAQDIYGPARWVTVPPLPGTLVINIGDLSHILSNGRFHSVLHRAVVN 233
           ILHQN+TSGLQV R         WVTVPP+ G LV+++GDL HILSNG + SVLHRA+VN
Sbjct: 225 ILHQNNTSGLQVQRE-----GMGWVTVPPIKGALVVHVGDLLHILSNGLYSSVLHRAIVN 279

Query: 232 RVCHRYSMAYICGPPAHAKVSPLRPLSG---RPVFRAVTWPEYLGLKGKLFNQALASIRV 62
           R  HR+S+AY+ GPP++ ++SPL  L+     P+++ VTW EYL +K K FN+ L+ +R+
Sbjct: 280 RTQHRFSIAYLYGPPSNVRISPLSKLTSIVRPPLYQPVTWSEYLAIKAKYFNKTLSVVRL 339

Query: 61  S 59
           S
Sbjct: 340 S 340


>gb|ACT99991.1| gibberellin 3-oxidase [Malus x domestica]
          Length = 369

 Score =  216 bits (550), Expect = 2e-54
 Identities = 109/180 (60%), Positives = 137/180 (76%), Gaps = 3/180 (1%)
 Frame = -1

Query: 592 RVMHLMLQSLGLNEDDLDWAGPVKDLQEVSGVLQLNSYPSCPDPDRAMGLAAHTDSSLLT 413
           R+M LML SLG++ +D+ WAG   + +  S  LQLNSYP+CP+PDRAMGLAAHTDS+LLT
Sbjct: 178 RLMWLMLGSLGISMEDIKWAGSKGEFKGASAALQLNSYPACPEPDRAMGLAAHTDSTLLT 237

Query: 412 ILHQNSTSGLQVLRAQDIYGPARWVTVPPLPGTLVINIGDLSHILSNGRFHSVLHRAVVN 233
           IL+Q++TSGLQVLR         WVTVPP+PG LVIN+GDL HILSNG + SVLHRAVVN
Sbjct: 238 ILYQSNTSGLQVLRE-----GTGWVTVPPMPGALVINVGDLFHILSNGLYPSVLHRAVVN 292

Query: 232 RVCHRYSMAYICGPPAHAKVSPLRPLSG---RPVFRAVTWPEYLGLKGKLFNQALASIRV 62
           R  HR S+AY+ GPPA  ++SPL  L G    P++R ++W EYLG K K FN+AL+S+R+
Sbjct: 293 RTQHRLSVAYLFGPPASIQISPLSKLVGPSHPPLYRPISWNEYLGTKAKHFNKALSSVRL 352