BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00024846
         (1126 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI35795.3| unnamed protein product [Vitis vinifera]              243   e-118
ref|XP_002265217.1| PREDICTED: pectinesterase 3 [Vitis vinifera]      243   e-118
ref|XP_002304256.1| predicted protein [Populus trichocarpa] gi|2...   236   e-118
sp|Q43111.1|PME3_PHAVU RecName: Full=Pectinesterase 3; Short=PE ...   231   e-118
ref|XP_003627449.1| Pectinesterase [Medicago truncatula] gi|3555...   234   e-117

>emb|CBI35795.3| unnamed protein product [Vitis vinifera]
          Length = 670

 Score =  243 bits (620), Expect(2) = e-118
 Identities = 115/158 (72%), Positives = 136/158 (86%)
 Frame = -1

Query: 1123 PNVTVAKDRSGDVMTVGEAVAMVPKKSKDQFVIYVKAGEYVENVVVDKNKWNVFIYGDGI 944
            P+VTVAKD +GD +T+ EAVAMVPKKS+ +FVIYVK G Y EN+++DK+KWNV IYGDG 
Sbjct: 362  PDVTVAKDGTGDYVTIKEAVAMVPKKSEKRFVIYVKEGNYSENIILDKSKWNVMIYGDGK 421

Query: 943  DKTIISGGRNFIDGTPTFSTATFVAVGRRFMAKGISFKNTAGPDKHQAVALRAGSDQSVF 764
            DK+I+SG  NFIDGTPTF+TATF AVG+ F+AK + F+NTAG  KHQAVA R+GSD SVF
Sbjct: 422  DKSIVSGNLNFIDGTPTFATATFAAVGKGFIAKYMRFENTAGAAKHQAVAFRSGSDMSVF 481

Query: 763  YKCSFDGYQDTLYAHSLRQFYRDCHITGTIDFIFGNAA 650
            Y+CSFD +QDTLYAHS RQFYR+C ITGTIDFIFGNAA
Sbjct: 482  YQCSFDAFQDTLYAHSNRQFYRECDITGTIDFIFGNAA 519



 Score =  211 bits (536), Expect(2) = e-118
 Identities = 95/157 (60%), Positives = 122/157 (77%)
 Frame = -3

Query: 578 IFGNAAVVFQSCNIKPRQPLPNQYNTITAQGRTDPNQNTGISIQSSTISALDSLTRPTYL 399
           IFGNAAVVFQ+C I+PRQP+ NQ+NTITAQG+ DPNQNTGISIQ  +ISAL++LT PTYL
Sbjct: 514 IFGNAAVVFQACKIQPRQPMSNQFNTITAQGKKDPNQNTGISIQKCSISALNTLTAPTYL 573

Query: 398 GRPWKPYSTTIIMKTEISAVVDPSGWMPWVAGTVPPETIMYAEYDNTGPGAAVAGRVKWP 219
           GRPWK YSTTI+M++ I + ++P GW  WV G  PP TI YAE+ NTGPGA +  RVKW 
Sbjct: 574 GRPWKAYSTTIVMQSNIGSFLNPKGWTEWVTGVDPPSTIFYAEFQNTGPGATLDQRVKWA 633

Query: 218 GYQSSVSDKVAQKFSVASFIAGDEWLPDSGVEFVSTL 108
           G+ +++++  A KF+V +FI G  WL +S V F ++L
Sbjct: 634 GFMTNITEDEAAKFTVGTFIQGASWLSESSVTFDASL 670



 Score =  159 bits (403), Expect(2) = 4e-72
 Identities = 84/186 (45%), Positives = 111/186 (59%), Gaps = 6/186 (3%)
 Frame = -3

Query: 578 IFGNAAVVFQSCNIKPRQPLPNQYNTITAQGRTDPNQNTGISIQSSTISALDSL-----T 414
           IFGNAA VFQ C+I  R+P   Q N +TAQGRTDPNQNTGI IQ   I A   L     +
Sbjct: 100 IFGNAAAVFQDCDIHARRPNSGQKNMLTAQGRTDPNQNTGIVIQKCRIGATSDLQAVISS 159

Query: 413 RPTYLGRPWKPYSTTIIMKTEISAVVDPSGWMPWVAGTVPPETIMYAEYDNTGPGAAVAG 234
             TYLGRPWK YS T++M+T I+ V+DP+GW  W +G+    T+ Y EY NTG GA  + 
Sbjct: 160 FKTYLGRPWKEYSRTVVMQTSITNVIDPAGWHEW-SGSFALSTLYYGEYQNTGAGAGTSK 218

Query: 233 RVKWPGYQSSVSDKVAQKFSVASFIAGDEWLPDSGVEFVSTLV-LNFFFSFGCFFYIVLI 57
           RV W G++   S   AQ F+  +FIAG  WL  +G  +   L      +++G F     +
Sbjct: 219 RVTWKGFKVITSASEAQGFTPGTFIAGSSWLGSTGFPYSLGLEHQKGIWTYGTFVRTTTL 278

Query: 56  IE*YID 39
           ++ Y+D
Sbjct: 279 LDAYMD 284



 Score =  139 bits (351), Expect(2) = 4e-72
 Identities = 64/102 (62%), Positives = 78/102 (76%)
 Frame = -1

Query: 955 GDGIDKTIISGGRNFIDGTPTFSTATFVAVGRRFMAKGISFKNTAGPDKHQAVALRAGSD 776
           GDG   TII+G +N +DG+ TF++AT   VG +F+A+ I+F+NTAGP KHQAVALR GSD
Sbjct: 4   GDGRSNTIITGSKNVVDGSTTFNSATVAVVGEKFIARDITFQNTAGPSKHQAVALRVGSD 63

Query: 775 QSVFYKCSFDGYQDTLYAHSLRQFYRDCHITGTIDFIFGNAA 650
            S FYKC    YQDTLY HS RQFY +C + GT+DFIFGNAA
Sbjct: 64  LSAFYKCDMLAYQDTLYVHSNRQFYINCLVAGTVDFIFGNAA 105


>ref|XP_002265217.1| PREDICTED: pectinesterase 3 [Vitis vinifera]
          Length = 578

 Score =  243 bits (620), Expect(2) = e-118
 Identities = 115/158 (72%), Positives = 136/158 (86%)
 Frame = -1

Query: 1123 PNVTVAKDRSGDVMTVGEAVAMVPKKSKDQFVIYVKAGEYVENVVVDKNKWNVFIYGDGI 944
            P+VTVAKD +GD +T+ EAVAMVPKKS+ +FVIYVK G Y EN+++DK+KWNV IYGDG 
Sbjct: 270  PDVTVAKDGTGDYVTIKEAVAMVPKKSEKRFVIYVKEGNYSENIILDKSKWNVMIYGDGK 329

Query: 943  DKTIISGGRNFIDGTPTFSTATFVAVGRRFMAKGISFKNTAGPDKHQAVALRAGSDQSVF 764
            DK+I+SG  NFIDGTPTF+TATF AVG+ F+AK + F+NTAG  KHQAVA R+GSD SVF
Sbjct: 330  DKSIVSGNLNFIDGTPTFATATFAAVGKGFIAKYMRFENTAGAAKHQAVAFRSGSDMSVF 389

Query: 763  YKCSFDGYQDTLYAHSLRQFYRDCHITGTIDFIFGNAA 650
            Y+CSFD +QDTLYAHS RQFYR+C ITGTIDFIFGNAA
Sbjct: 390  YQCSFDAFQDTLYAHSNRQFYRECDITGTIDFIFGNAA 427



 Score =  211 bits (536), Expect(2) = e-118
 Identities = 95/157 (60%), Positives = 122/157 (77%)
 Frame = -3

Query: 578 IFGNAAVVFQSCNIKPRQPLPNQYNTITAQGRTDPNQNTGISIQSSTISALDSLTRPTYL 399
           IFGNAAVVFQ+C I+PRQP+ NQ+NTITAQG+ DPNQNTGISIQ  +ISAL++LT PTYL
Sbjct: 422 IFGNAAVVFQACKIQPRQPMSNQFNTITAQGKKDPNQNTGISIQKCSISALNTLTAPTYL 481

Query: 398 GRPWKPYSTTIIMKTEISAVVDPSGWMPWVAGTVPPETIMYAEYDNTGPGAAVAGRVKWP 219
           GRPWK YSTTI+M++ I + ++P GW  WV G  PP TI YAE+ NTGPGA +  RVKW 
Sbjct: 482 GRPWKAYSTTIVMQSNIGSFLNPKGWTEWVTGVDPPSTIFYAEFQNTGPGATLDQRVKWA 541

Query: 218 GYQSSVSDKVAQKFSVASFIAGDEWLPDSGVEFVSTL 108
           G+ +++++  A KF+V +FI G  WL +S V F ++L
Sbjct: 542 GFMTNITEDEAAKFTVGTFIQGASWLSESSVTFDASL 578


>ref|XP_002304256.1| predicted protein [Populus trichocarpa] gi|222841688|gb|EEE79235.1|
            predicted protein [Populus trichocarpa]
          Length = 588

 Score =  236 bits (602), Expect(2) = e-118
 Identities = 110/158 (69%), Positives = 130/158 (82%)
 Frame = -1

Query: 1123 PNVTVAKDRSGDVMTVGEAVAMVPKKSKDQFVIYVKAGEYVENVVVDKNKWNVFIYGDGI 944
            PNVTVAKD SGD  T+ EAV  +PKKS+ +F+I+VK G YVENV++DK+KWNV IYG+G 
Sbjct: 280  PNVTVAKDGSGDCETLREAVGKIPKKSESKFIIHVKEGVYVENVILDKSKWNVMIYGEGK 339

Query: 943  DKTIISGGRNFIDGTPTFSTATFVAVGRRFMAKGISFKNTAGPDKHQAVALRAGSDQSVF 764
            DKTI+SG  NF+DGTPTFST TF   G+ F A+ + F NTAG +KHQAVA R+GSD SVF
Sbjct: 340  DKTIVSGSLNFVDGTPTFSTPTFAVAGKGFFARDMKFINTAGAEKHQAVAFRSGSDMSVF 399

Query: 763  YKCSFDGYQDTLYAHSLRQFYRDCHITGTIDFIFGNAA 650
            Y+C+FD +QDTLYAHS RQFYRDC ITGTIDFIFGNAA
Sbjct: 400  YRCAFDAFQDTLYAHSNRQFYRDCDITGTIDFIFGNAA 437



 Score =  215 bits (547), Expect(2) = e-118
 Identities = 101/157 (64%), Positives = 121/157 (77%)
 Frame = -3

Query: 578 IFGNAAVVFQSCNIKPRQPLPNQYNTITAQGRTDPNQNTGISIQSSTISALDSLTRPTYL 399
           IFGNAAVVFQ+CNI+PRQPL NQ+NTITAQG+ DPNQNTGISIQ    SA D++T PTYL
Sbjct: 432 IFGNAAVVFQNCNIQPRQPLANQFNTITAQGKKDPNQNTGISIQKCKFSAFDNVTAPTYL 491

Query: 398 GRPWKPYSTTIIMKTEISAVVDPSGWMPWVAGTVPPETIMYAEYDNTGPGAAVAGRVKWP 219
           GRPWK YSTT+IM+++I + + P GW+ WV+G  PP TI YAEY NTG GA V GRVKW 
Sbjct: 492 GRPWKDYSTTVIMQSDIGSFLRPLGWISWVSGVDPPATIFYAEYQNTGSGANVDGRVKWT 551

Query: 218 GYQSSVSDKVAQKFSVASFIAGDEWLPDSGVEFVSTL 108
           GY+ +++   A KF+V SFI G EWLP + V F S L
Sbjct: 552 GYKPALTVDEAGKFAVDSFIQGSEWLPKTSVTFQSML 588


>sp|Q43111.1|PME3_PHAVU RecName: Full=Pectinesterase 3; Short=PE 3; AltName: Full=Pectin
            methylesterase 3; Flags: Precursor
            gi|732913|emb|CAA59482.1| pectinesterase [Phaseolus
            vulgaris]
          Length = 581

 Score =  231 bits (589), Expect(2) = e-118
 Identities = 108/158 (68%), Positives = 127/158 (80%)
 Frame = -1

Query: 1123 PNVTVAKDRSGDVMTVGEAVAMVPKKSKDQFVIYVKAGEYVENVVVDKNKWNVFIYGDGI 944
            P+  VAKD SG   T+GEA+ +V KKS+++F +YVK G YVEN+ +DKN WNV IYGDG 
Sbjct: 272  PDAVVAKDGSGQFKTIGEALKLVKKKSEERFSVYVKEGRYVENIDLDKNTWNVMIYGDGK 331

Query: 943  DKTIISGGRNFIDGTPTFSTATFVAVGRRFMAKGISFKNTAGPDKHQAVALRAGSDQSVF 764
            DKT + G RNF+DGTPTF TATF   G+ F+AK I F N AG  KHQAVALR+GSD+SVF
Sbjct: 332  DKTFVVGSRNFMDGTPTFETATFAVKGKGFIAKDIGFVNNAGASKHQAVALRSGSDRSVF 391

Query: 763  YKCSFDGYQDTLYAHSLRQFYRDCHITGTIDFIFGNAA 650
            ++CSFDG+QDTLYAHS RQFYRDC ITGTIDFIFGNAA
Sbjct: 392  FRCSFDGFQDTLYAHSNRQFYRDCDITGTIDFIFGNAA 429



 Score =  220 bits (560), Expect(2) = e-118
 Identities = 102/158 (64%), Positives = 128/158 (81%), Gaps = 1/158 (0%)
 Frame = -3

Query: 578 IFGNAAVVFQSCNIKPRQPLPNQYNTITAQGRTDPNQNTGISIQSSTISAL-DSLTRPTY 402
           IFGNAAVVFQSC I PRQPLPNQ+NTITAQG+ DPNQNTGI IQ STI+   ++LT PTY
Sbjct: 424 IFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFGNNLTAPTY 483

Query: 401 LGRPWKPYSTTIIMKTEISAVVDPSGWMPWVAGTVPPETIMYAEYDNTGPGAAVAGRVKW 222
           LGRPWK +STT+IM+++I A+++P GWM WV    PP TI YAEY N+GPGA V+ RVKW
Sbjct: 484 LGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGADVSQRVKW 543

Query: 221 PGYQSSVSDKVAQKFSVASFIAGDEWLPDSGVEFVSTL 108
            GY+ +++D+ A++F+V SFI G EWLP++ V+F STL
Sbjct: 544 AGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581


>ref|XP_003627449.1| Pectinesterase [Medicago truncatula] gi|355521471|gb|AET01925.1|
            Pectinesterase [Medicago truncatula]
          Length = 582

 Score =  234 bits (597), Expect(2) = e-117
 Identities = 110/159 (69%), Positives = 129/159 (81%)
 Frame = -1

Query: 1126 LPNVTVAKDRSGDVMTVGEAVAMVPKKSKDQFVIYVKAGEYVENVVVDKNKWNVFIYGDG 947
            +P+  VAKD SG   T+GEA+ +V KKS  +FV+YVK G YVEN+ +DKN WNV IYGDG
Sbjct: 271  VPDAVVAKDGSGQYKTIGEALKLVKKKSLQRFVVYVKKGVYVENIDLDKNTWNVMIYGDG 330

Query: 946  IDKTIISGGRNFIDGTPTFSTATFVAVGRRFMAKGISFKNTAGPDKHQAVALRAGSDQSV 767
            + +T++SG RN+IDGTPTF TATF   G+ F+AK I F NTAG  KHQAVA+R+GSDQSV
Sbjct: 331  MTETVVSGSRNYIDGTPTFETATFAVKGKGFIAKDIQFLNTAGASKHQAVAMRSGSDQSV 390

Query: 766  FYKCSFDGYQDTLYAHSLRQFYRDCHITGTIDFIFGNAA 650
            FY+CSF GYQDTLYAHS RQFYRDC ITGTIDFIFGNAA
Sbjct: 391  FYRCSFVGYQDTLYAHSNRQFYRDCDITGTIDFIFGNAA 429



 Score =  214 bits (545), Expect(2) = e-117
 Identities = 101/159 (63%), Positives = 123/159 (77%), Gaps = 2/159 (1%)
 Frame = -3

Query: 578 IFGNAAVVFQSCNIKPRQPLPNQYNTITAQGRTDPNQNTGISIQSSTISAL--DSLTRPT 405
           IFGNAA VFQ+C I PRQP+ NQ+NTITAQG+ DPNQN+GI IQ ST + L  D+L  PT
Sbjct: 424 IFGNAAAVFQNCKIMPRQPMSNQFNTITAQGKKDPNQNSGIVIQKSTFTTLPGDNLIAPT 483

Query: 404 YLGRPWKPYSTTIIMKTEISAVVDPSGWMPWVAGTVPPETIMYAEYDNTGPGAAVAGRVK 225
           YLGRPWK +STTIIMK+EI + + P GW+ WVA   PP +I+YAEY NTGPGA VAGRVK
Sbjct: 484 YLGRPWKDFSTTIIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVAGRVK 543

Query: 224 WPGYQSSVSDKVAQKFSVASFIAGDEWLPDSGVEFVSTL 108
           W GY+ ++ D+ A KF+V SFI G EWLP + V+F ST+
Sbjct: 544 WAGYKPALGDEDAIKFTVDSFIQGPEWLPSASVQFDSTI 582