BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00019907
         (1077 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABK28642.1| unknown [Arabidopsis thaliana]                         361   1e-97
ref|NP_193852.2| gibberellin 2-beta-dioxygenase 8 [Arabidopsis t...   361   1e-97
ref|XP_003610659.1| Gibberellin 2-beta-dioxygenase [Medicago tru...   360   4e-97
gb|AAO92303.1| gibberellin 2-oxidase 1 [Nicotiana sylvestris]         359   7e-97
ref|XP_002518538.1| gibberellin 20-oxidase, putative [Ricinus co...   358   1e-96

>gb|ABK28642.1| unknown [Arabidopsis thaliana]
          Length = 339

 Score =  361 bits (927), Expect = 1e-97
 Identities = 175/302 (57%), Positives = 219/302 (72%), Gaps = 5/302 (1%)
 Frame = -2

Query: 1028 ASVEECELPLIDLSLLYT---ENASRCKREIAAASQNWGFFQVVNHGISSELLHRIRSEQ 858
            A VEE ELP+ID+S L     E   +CK  IA AS+ WGFFQV+NHGIS ++L ++R EQ
Sbjct: 35   AVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQ 94

Query: 857  VKLFRQPFENKCKD--FSADAYRWGTPTATSLHQLSWSEAYHIPLCQSTDPSKHNTIISR 684
            +++FR+PF+ K K   FSA +YRWGTP+ATS+ QLSWSEA+H+P+   +D     T+ S 
Sbjct: 95   IRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNKDFTTLSS- 153

Query: 683  LTIEEXXXXXXXXXXXXXXXXXXXXXXXXAYFERNCNRETCYLRLNRYPPCPVPGGLFGL 504
             T+E+                        ++F+ NC R TCYLR+NRYPPCP P  ++GL
Sbjct: 154  -TMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGL 212

Query: 503  VPHTDSDFLTILCQDEVGGLQLMKDGRWITVKPNPSALVVNIGDLFQAWSNGMYKSVEHR 324
            +PHTDSDFLTIL QD+VGGLQL+KD RWI VKPNP AL++NIGDLFQAWSNGMYKSVEHR
Sbjct: 213  MPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHR 272

Query: 323  VISNESQERFSVAYFMCPSNDSMIESEAEKGIYRRFSFREYRQQVQEDVKMVGYKVGLSR 144
            V++N   ERFS AYFMCPS D++IE  +++  YR FSFRE+RQQVQEDVK  G+KVGL R
Sbjct: 273  VMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPR 332

Query: 143  FL 138
            FL
Sbjct: 333  FL 334


>ref|NP_193852.2| gibberellin 2-beta-dioxygenase 8 [Arabidopsis thaliana]
            gi|259016235|sp|O49561.2|G2OX8_ARATH RecName:
            Full=Gibberellin 2-beta-dioxygenase 8; AltName: Full=GA
            2-oxidase 8; AltName: Full=Gibberellin 2-beta-hydroxylase
            8; AltName: Full=Gibberellin 2-oxidase 8
            gi|91806706|gb|ABE66080.1| oxidoreductase 2OG-Fe(II)
            oxygenase family protein [Arabidopsis thaliana]
            gi|332659020|gb|AEE84420.1| gibberellin
            2-beta-dioxygenase 8 [Arabidopsis thaliana]
          Length = 338

 Score =  361 bits (927), Expect = 1e-97
 Identities = 175/302 (57%), Positives = 219/302 (72%), Gaps = 5/302 (1%)
 Frame = -2

Query: 1028 ASVEECELPLIDLSLLYT---ENASRCKREIAAASQNWGFFQVVNHGISSELLHRIRSEQ 858
            A VEE ELP+ID+S L     E   +CK  IA AS+ WGFFQV+NHGIS ++L ++R EQ
Sbjct: 35   AVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQ 94

Query: 857  VKLFRQPFENKCKD--FSADAYRWGTPTATSLHQLSWSEAYHIPLCQSTDPSKHNTIISR 684
            +++FR+PF+ K K   FSA +YRWGTP+ATS+ QLSWSEA+H+P+   +D     T+ S 
Sbjct: 95   IRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNKDFTTLSS- 153

Query: 683  LTIEEXXXXXXXXXXXXXXXXXXXXXXXXAYFERNCNRETCYLRLNRYPPCPVPGGLFGL 504
             T+E+                        ++F+ NC R TCYLR+NRYPPCP P  ++GL
Sbjct: 154  -TMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEVYGL 212

Query: 503  VPHTDSDFLTILCQDEVGGLQLMKDGRWITVKPNPSALVVNIGDLFQAWSNGMYKSVEHR 324
            +PHTDSDFLTIL QD+VGGLQL+KD RWI VKPNP AL++NIGDLFQAWSNGMYKSVEHR
Sbjct: 213  MPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSVEHR 272

Query: 323  VISNESQERFSVAYFMCPSNDSMIESEAEKGIYRRFSFREYRQQVQEDVKMVGYKVGLSR 144
            V++N   ERFS AYFMCPS D++IE  +++  YR FSFRE+RQQVQEDVK  G+KVGL R
Sbjct: 273  VMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVGLPR 332

Query: 143  FL 138
            FL
Sbjct: 333  FL 334


>ref|XP_003610659.1| Gibberellin 2-beta-dioxygenase [Medicago truncatula]
            gi|355511994|gb|AES93617.1| Gibberellin
            2-beta-dioxygenase [Medicago truncatula]
          Length = 332

 Score =  360 bits (923), Expect = 4e-97
 Identities = 183/323 (56%), Positives = 229/323 (70%), Gaps = 9/323 (2%)
 Frame = -2

Query: 1076 TYKQLLKTQPSCSHDLASVE-ECELPLIDLSLLYTENA---SRCKREIAAASQNWGFFQV 909
            TYK L     +  +D   V+ ECELP+IDLS L  ++      CK  IA ASQ WGF QV
Sbjct: 13   TYKNLFNKDQNIINDELMVDNECELPVIDLSRLNDDDEVAREECKSMIANASQEWGFLQV 72

Query: 908  VNHGISSELLHRIRSEQVKLFRQPFENKCKD-----FSADAYRWGTPTATSLHQLSWSEA 744
            VNHGISS++L R+R EQ K+F++PF+ K K+     FSA +YRWGTPTAT + QLSWSEA
Sbjct: 73   VNHGISSDILTRLRCEQKKVFKEPFDKKTKEDKFLNFSAGSYRWGTPTATCIKQLSWSEA 132

Query: 743  YHIPLCQSTDPSKHNTIISRLTIEEXXXXXXXXXXXXXXXXXXXXXXXXAYFERNCNRET 564
            +HIPL   TD    NT +S + IE+                        ++F+ NC   T
Sbjct: 133  FHIPL---TDILGSNTHLSSI-IEQFATTVSNLAQILANILAEKLGHQSSFFKENCLPNT 188

Query: 563  CYLRLNRYPPCPVPGGLFGLVPHTDSDFLTILCQDEVGGLQLMKDGRWITVKPNPSALVV 384
            CYLRLNRYPPCP+   + GL+PHTDSDFLTIL QD+VGGLQL+KDG+W+ VKPNP AL++
Sbjct: 189  CYLRLNRYPPCPIDFRIHGLMPHTDSDFLTILYQDQVGGLQLVKDGKWVAVKPNPDALII 248

Query: 383  NIGDLFQAWSNGMYKSVEHRVISNESQERFSVAYFMCPSNDSMIESEAEKGIYRRFSFRE 204
            NIGDLFQAWSNG+YKSVEHRV++N   ERFSVAYF+CPSND+MIES  E  IYR+FSF+E
Sbjct: 249  NIGDLFQAWSNGVYKSVEHRVVTNPRVERFSVAYFLCPSNDTMIESCKEPSIYRKFSFKE 308

Query: 203  YRQQVQEDVKMVGYKVGLSRFLV 135
            YRQQV++DV+ +G K+GL RF++
Sbjct: 309  YRQQVRDDVQKLGSKIGLPRFII 331


>gb|AAO92303.1| gibberellin 2-oxidase 1 [Nicotiana sylvestris]
          Length = 354

 Score =  359 bits (921), Expect = 7e-97
 Identities = 182/326 (55%), Positives = 228/326 (69%), Gaps = 12/326 (3%)
 Frame = -2

Query: 1076 TYKQLL------KTQPSCSHDLASVEECELPLIDLSLLYTENASR--CKREIAAASQNWG 921
            TYK+L       K      H+L  VEECELPLIDL LL      R  CKR+IA ASQ WG
Sbjct: 28   TYKKLFDDIRIGKASNINYHNLFVVEECELPLIDLELLNGREFEREECKRKIAKASQEWG 87

Query: 920  FFQVVNHGISSELLHRIRSEQVKLFRQPFENKCKD----FSADAYRWGTPTATSLHQLSW 753
            FFQVVNHGIS ++L ++R EQ+KLF++PF  K  +    FS  +YRWGTP+AT L QLSW
Sbjct: 88   FFQVVNHGISHDILEKMRMEQIKLFKKPFNEKMNEKNHKFSKGSYRWGTPSATCLQQLSW 147

Query: 752  SEAYHIPLCQSTDPSKHNTIISRLTIEEXXXXXXXXXXXXXXXXXXXXXXXXAYFERNCN 573
            SEA+H+PL   ++ S  +++ +  T+E+                         YF   C 
Sbjct: 148  SEAFHVPLTDISNSSGVSSLST--TMEQYATTLSELAHKLARILSEKMGYKSKYFRETCL 205

Query: 572  RETCYLRLNRYPPCPVPGGLFGLVPHTDSDFLTILCQDEVGGLQLMKDGRWITVKPNPSA 393
              TCYLR+NRYP CP+   +FGL+PHTDSDFLTIL QDE+GGLQL++DG+WI+VKPNP A
Sbjct: 206  VNTCYLRMNRYPACPLSPQVFGLMPHTDSDFLTILHQDEIGGLQLVRDGKWISVKPNPQA 265

Query: 392  LVVNIGDLFQAWSNGMYKSVEHRVISNESQERFSVAYFMCPSNDSMIESEAEKGIYRRFS 213
            L++NIGDLFQAWSNG+YKSVEHRV++N+++ERFS A+F+CPS D+ I S  E  +YRRF+
Sbjct: 266  LIINIGDLFQAWSNGVYKSVEHRVVTNKAKERFSTAFFLCPSYDTEIRSCFEPPVYRRFT 325

Query: 212  FREYRQQVQEDVKMVGYKVGLSRFLV 135
            FRE+RQQVQEDVK  GYKVGL RFLV
Sbjct: 326  FREFRQQVQEDVKNFGYKVGLPRFLV 351


>ref|XP_002518538.1| gibberellin 20-oxidase, putative [Ricinus communis]
            gi|223542383|gb|EEF43925.1| gibberellin 20-oxidase,
            putative [Ricinus communis]
          Length = 331

 Score =  358 bits (920), Expect = 1e-96
 Identities = 182/323 (56%), Positives = 226/323 (69%), Gaps = 10/323 (3%)
 Frame = -2

Query: 1073 YKQLLKTQPSCSHDLAS--VEECELPLIDLSLLYTENA---SRCKREIAAASQNWGFFQV 909
            YK LL ++     +  S  VEECELPLIDL  L  ++     +CK+EIA ASQ+WGFFQV
Sbjct: 11   YKTLLNSKTIQESETKSSIVEECELPLIDLGRLSMDDELEKDKCKQEIARASQDWGFFQV 70

Query: 908  VNHGISSELLHRIRSEQVKLFRQPFENKCKD-----FSADAYRWGTPTATSLHQLSWSEA 744
            VNHGIS E+L ++RSEQVKLF+QPF  K K+     FSA  YRWGTPTAT L QLSWSEA
Sbjct: 71   VNHGISREILEKMRSEQVKLFKQPFYQKSKEDKFLKFSAGTYRWGTPTATCLSQLSWSEA 130

Query: 743  YHIPLCQSTDPSKHNTIISRLTIEEXXXXXXXXXXXXXXXXXXXXXXXXAYFERNCNRET 564
            +HIP+   +  +   T  S  T+E+                         +FE NC   T
Sbjct: 131  FHIPMTDISASNGFTTFSS--TMEQFATTVANLAQKLAGILAEKLDRKSTFFEENCLPST 188

Query: 563  CYLRLNRYPPCPVPGGLFGLVPHTDSDFLTILCQDEVGGLQLMKDGRWITVKPNPSALVV 384
            CYLRLNRYPPCP+P  + GL+PHTDSDFLTIL QD+VGGLQL+KD +W  VKPNP AL++
Sbjct: 189  CYLRLNRYPPCPIPVEVCGLMPHTDSDFLTILHQDQVGGLQLVKDRKWFAVKPNPEALII 248

Query: 383  NIGDLFQAWSNGMYKSVEHRVISNESQERFSVAYFMCPSNDSMIESEAEKGIYRRFSFRE 204
            NIGDLFQAWSN +YKSV+HRV++N   ERFS AYF CPS D+ IES  E  +Y++FSF+E
Sbjct: 249  NIGDLFQAWSNDVYKSVQHRVVTNPGVERFSTAYFFCPSYDTEIESGCEPSVYKKFSFQE 308

Query: 203  YRQQVQEDVKMVGYKVGLSRFLV 135
            YR+QVQ+DV+++G+KVGL RF+V
Sbjct: 309  YREQVQKDVQILGHKVGLLRFIV 331