BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00019632
         (709 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003566953.1| PREDICTED: uncharacterized hydroxylase C887....   258   8e-67
dbj|BAB63743.1| acid phosphatase-like [Oryza sativa Japonica Gro...   249   4e-64
ref|XP_002275229.1| PREDICTED: sphingoid base hydroxylase 2 [Vit...   245   7e-63
ref|NP_563944.1| sphingoid base hydroxylase 2 [Arabidopsis thali...   244   1e-62
gb|AAF43928.1|AC012188_5 Contains similarity to acid phosphatase...   243   2e-62

>ref|XP_003566953.1| PREDICTED: uncharacterized hydroxylase C887.15c-like [Brachypodium
           distachyon]
          Length = 263

 Score =  258 bits (659), Expect = 8e-67
 Identities = 128/220 (58%), Positives = 153/220 (69%), Gaps = 6/220 (2%)
 Frame = -1

Query: 646 FFSSDEFMVMFVPIIVYWVYSGLYEMLGS---LDNYRLHSRRDEETKNLVSKLDVFKGVL 476
           F   +E +V   P+ VYW+YSG+YE LGS   LD YRLHSRRDEETKN+ SK DV KGVL
Sbjct: 3   FIGGEELLVTLAPVAVYWLYSGIYEALGSSEALDRYRLHSRRDEETKNMASKKDVVKGVL 62

Query: 475 FQQSLQAAATFLIFKLT--NDPXXXXXXXXXXXXXSFPTLARQIFVAMFVLDSWQYMVHR 302
            QQ++QAA +  + KLT  ++              SF  +A +  VAM VLD+WQY +HR
Sbjct: 63  LQQAIQAAISVAVLKLTGGDEQISGGVKAHDSSSSSFLEVAARFGVAMAVLDAWQYFMHR 122

Query: 301 CMHSNKFLYRNFHSWHHRVVAPYAFAAQYNHPLDGFLTEALSGAMAFFISGMSARXXXXX 122
            MHS++FLYR FHSWHHRVVAPYAFAAQYNHP+DG LTE LSGA AF  SGM  R     
Sbjct: 123 LMHSSRFLYRRFHSWHHRVVAPYAFAAQYNHPVDGVLTETLSGAAAFLASGMGPRAAAVF 182

Query: 121 XXXXXIKGIDDHCGLVLPWNPFQLLF-SNNTAYHDVHHQL 5
                +KGIDDHCG+++PWNP  ++F  NNTAYHDVHHQL
Sbjct: 183 FVFATVKGIDDHCGVLVPWNPIHVVFRDNNTAYHDVHHQL 222


>dbj|BAB63743.1| acid phosphatase-like [Oryza sativa Japonica Group]
           gi|18844788|dbj|BAB85258.1| acid phosphatase-like [Oryza
           sativa Japonica Group] gi|125527131|gb|EAY75245.1|
           hypothetical protein OsI_03133 [Oryza sativa Indica
           Group] gi|125571450|gb|EAZ12965.1| hypothetical protein
           OsJ_02886 [Oryza sativa Japonica Group]
          Length = 283

 Score =  249 bits (636), Expect = 4e-64
 Identities = 122/231 (52%), Positives = 150/231 (64%), Gaps = 16/231 (6%)
 Frame = -1

Query: 646 FFSSDEFMVMFVPIIVYWVYSGLYEMLGS---LDNYRLHSRRDEETKNLVSKLDVFKGVL 476
           F S +E  V   P+ VYW+YSG+YE LGS   LD YRLHSRRDEE+ N+ SK +V KGVL
Sbjct: 3   FISGEELAVTLAPVAVYWLYSGIYEALGSVRALDRYRLHSRRDEESNNMASKKEVVKGVL 62

Query: 475 FQQSLQAAATFLIFKLTNDPXXXXXXXXXXXXXS-------------FPTLARQIFVAMF 335
            QQ++Q A +  + KLT++              +                +A +  VAMF
Sbjct: 63  LQQAIQVAISLAVLKLTSEKDGGAGDVKAGHASAPAAAPSSSSAAAALLDVAARFGVAMF 122

Query: 334 VLDSWQYMVHRCMHSNKFLYRNFHSWHHRVVAPYAFAAQYNHPLDGFLTEALSGAMAFFI 155
           VLD+WQY  HR MHS++++YR FHSWHHRVVAPYAFAAQY HP+DG LTEALSGA A+  
Sbjct: 123 VLDAWQYFAHRLMHSSRYMYRRFHSWHHRVVAPYAFAAQYGHPVDGVLTEALSGAAAYLA 182

Query: 154 SGMSARXXXXXXXXXXIKGIDDHCGLVLPWNPFQLLFSNNTAYHDVHHQLS 2
           SG+  R          +KGIDDHCGL++PWNP    F+NNTAYHDVHHQLS
Sbjct: 183 SGLPPRAAAFFLAFATVKGIDDHCGLLVPWNPLHAAFANNTAYHDVHHQLS 233


>ref|XP_002275229.1| PREDICTED: sphingoid base hydroxylase 2 [Vitis vinifera]
           gi|296086379|emb|CBI31968.3| unnamed protein product
           [Vitis vinifera]
          Length = 258

 Score =  245 bits (625), Expect = 7e-63
 Identities = 120/214 (56%), Positives = 145/214 (67%)
 Frame = -1

Query: 646 FFSSDEFMVMFVPIIVYWVYSGLYEMLGSLDNYRLHSRRDEETKNLVSKLDVFKGVLFQQ 467
           F  SDE +  FVPI+VYWVYSG+Y  LGSL+NYRLHS+++EE KNLVSK  V KGV+ QQ
Sbjct: 3   FEVSDELLGAFVPILVYWVYSGIYIPLGSLENYRLHSKKEEEDKNLVSKGTVVKGVILQQ 62

Query: 466 SLQAAATFLIFKLTNDPXXXXXXXXXXXXXSFPTLARQIFVAMFVLDSWQYMVHRCMHSN 287
            +QA    L+F +T D                  LARQ F+AMFVLD+WQY +HR MH N
Sbjct: 63  VIQAVVAILLFTVTGDDSGAAAGPKPSLI----VLARQFFIAMFVLDTWQYFMHRYMHHN 118

Query: 286 KFLYRNFHSWHHRVVAPYAFAAQYNHPLDGFLTEALSGAMAFFISGMSARXXXXXXXXXX 107
           KFLYR+ HS HHR+V PYAF A YNHPL+G L + + GA++F +SGMS R          
Sbjct: 119 KFLYRHIHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLLSGMSPRASIFFFSFAT 178

Query: 106 IKGIDDHCGLVLPWNPFQLLFSNNTAYHDVHHQL 5
           IK +DDHCGL LP N F L F NN+AYHD+HHQL
Sbjct: 179 IKTVDDHCGLWLPGNLFHLFFRNNSAYHDIHHQL 212


>ref|NP_563944.1| sphingoid base hydroxylase 2 [Arabidopsis thaliana]
           gi|75261765|sp|Q9AST3.1|SBH2_ARATH RecName:
           Full=Sphinganine C(4)-monooxygenase 2; AltName:
           Full=Sphingoid C4-hydroxylase 2; AltName: Full=Sphingoid
           base hydroxylase 2 gi|13605750|gb|AAK32868.1|AF361856_1
           At1g14290/F14L17_4 [Arabidopsis thaliana]
           gi|24797014|gb|AAN64519.1| At1g14290/F14L17_4
           [Arabidopsis thaliana] gi|332191018|gb|AEE29139.1|
           sphingoid base hydroxylase 2 [Arabidopsis thaliana]
          Length = 259

 Score =  244 bits (623), Expect = 1e-62
 Identities = 118/217 (54%), Positives = 146/217 (67%)
 Frame = -1

Query: 655 MDEFFSSDEFMVMFVPIIVYWVYSGLYEMLGSLDNYRLHSRRDEETKNLVSKLDVFKGVL 476
           M  F  SDEF+  FVPI+VYWVYSG+Y  LGSLD YRLHS+ DE+ KNLVSK  V KGVL
Sbjct: 1   MMSFVISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVL 60

Query: 475 FQQSLQAAATFLIFKLTNDPXXXXXXXXXXXXXSFPTLARQIFVAMFVLDSWQYMVHRCM 296
            QQ+LQA  + ++FK+T                    LARQ  +AM V+D+WQY +HR M
Sbjct: 61  LQQTLQAIISVILFKITGSDADAATTQQFSILL----LARQFIIAMLVIDTWQYFIHRYM 116

Query: 295 HSNKFLYRNFHSWHHRVVAPYAFAAQYNHPLDGFLTEALSGAMAFFISGMSARXXXXXXX 116
           H NKFLY++ HS HHR++ PY++ A YNHPL+G L + + GA++F  SGMS R       
Sbjct: 117 HLNKFLYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFS 176

Query: 115 XXXIKGIDDHCGLVLPWNPFQLLFSNNTAYHDVHHQL 5
              IK +DDHCGL LP NPF + FSNN+AYHDVHHQL
Sbjct: 177 FATIKTVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQL 213


>gb|AAF43928.1|AC012188_5 Contains similarity to acid phosphatase from Lupinus albus
           gb|AB023385 and contains a Sterol desaturase PF|01598
           domain. EST gb|AI995340 comes from this gene
           [Arabidopsis thaliana]
          Length = 258

 Score =  243 bits (621), Expect = 2e-62
 Identities = 117/214 (54%), Positives = 145/214 (67%)
 Frame = -1

Query: 646 FFSSDEFMVMFVPIIVYWVYSGLYEMLGSLDNYRLHSRRDEETKNLVSKLDVFKGVLFQQ 467
           F  SDEF+  FVPI+VYWVYSG+Y  LGSLD YRLHS+ DE+ KNLVSK  V KGVL QQ
Sbjct: 3   FVISDEFLGTFVPILVYWVYSGMYICLGSLDKYRLHSKIDEDEKNLVSKSAVVKGVLLQQ 62

Query: 466 SLQAAATFLIFKLTNDPXXXXXXXXXXXXXSFPTLARQIFVAMFVLDSWQYMVHRCMHSN 287
           +LQA  + ++FK+T                    LARQ  +AM V+D+WQY +HR MH N
Sbjct: 63  TLQAIISVILFKITGSDADAATTQQFSILL----LARQFIIAMLVIDTWQYFIHRYMHLN 118

Query: 286 KFLYRNFHSWHHRVVAPYAFAAQYNHPLDGFLTEALSGAMAFFISGMSARXXXXXXXXXX 107
           KFLY++ HS HHR++ PY++ A YNHPL+G L + + GA++F  SGMS R          
Sbjct: 119 KFLYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGGALSFLFSGMSPRTAIFFFSFAT 178

Query: 106 IKGIDDHCGLVLPWNPFQLLFSNNTAYHDVHHQL 5
           IK +DDHCGL LP NPF + FSNN+AYHDVHHQL
Sbjct: 179 IKTVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQL 212