BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00017196
         (871 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAK64099.1| flavonoid 3',5'-hydroxylase [Eustoma exaltatum s...   295   7e-78
sp|Q96418.1|C75A5_EUSGR RecName: Full=Flavonoid 3',5'-hydroxylas...   295   7e-78
ref|XP_002314004.1| flavonoid 3',5'-hydroxylase [Populus trichoc...   295   9e-78
ref|XP_002314003.1| cytochrome P450 flavonoid 3',5'-hydroxylase ...   295   9e-78
sp|O04790.1|C75A7_EUSGR RecName: Full=Flavonoid 3',5'-hydroxylas...   294   2e-77

>dbj|BAK64099.1| flavonoid 3',5'-hydroxylase [Eustoma exaltatum subsp. russellianum]
          Length = 510

 Score =  295 bits (756), Expect = 7e-78
 Identities = 150/289 (51%), Positives = 198/289 (68%)
 Frame = +3

Query: 3   PIMYLKLGXXXXXXXXXXXXXXXFLTTFDHKFANRPTATMKAKEAAYNGDDMVFSNYTPR 182
           P+MYLK+G               FL T D  F+NRP     A   AYN  DMVF++Y PR
Sbjct: 70  PVMYLKVGSCGLAVASTPEAAKAFLKTLDMNFSNRPP-NAGATHLAYNAQDMVFADYGPR 128

Query: 183 WKLLRRLSTIHMLGNKAFTKWADVRRSEVMHMLQAMHESSKLGKPVLVPEMLICTTTNII 362
           WKLLR+LS IH+LG KA   W +VR+ E+ +ML AM ES + G+PV+V EML     N++
Sbjct: 129 WKLLRKLSNIHILGGKALQGWEEVRKKELGYMLYAMAESGRHGQPVVVSEMLTYAMANML 188

Query: 363 GRVMLSQRVFDSVDEELGKFRNVLKELMTGAGMFNIGDFVPGIGWMDLQGVKAKLVKAHR 542
           G+VMLS+RVF S   E  +F++++ ELMT AG FNIGDF+P I WMDLQG++  + + H+
Sbjct: 189 GQVMLSKRVFGSQGSESNEFKDMVVELMTVAGYFNIGDFIPSIAWMDLQGIQGGMKRLHK 248

Query: 543 KMDDMMKRMLALHKATMKEREGCQDFIDLVLCSELRDDDGEKLSDVNIRALLSNMFTAGT 722
           K D ++ R+L  H A+  ER+G  DF+D V+ +   + +GE+L  VNI+ALL NMFTAGT
Sbjct: 249 KFDALLTRLLEEHTASAHERKGSPDFLDFVVANR-DNSEGERLHTVNIKALLLNMFTAGT 307

Query: 723 DTSTIIVEWALAELIKNPIILKRLQSEMDAIIGQERILQEDDISNLPYL 869
           DTS+ ++EWALAEL+KNPIILKR Q EMD +IG++R   E DIS LPYL
Sbjct: 308 DTSSSVIEWALAELLKNPIILKRAQEEMDGVIGRDRRFLEADISKLPYL 356


>sp|Q96418.1|C75A5_EUSGR RecName: Full=Flavonoid 3',5'-hydroxylase; Short=F3'5'H; AltName:
           Full=Cytochrome P450 75A5 gi|1644388|gb|AAB17562.1|
           flavonoid 3'5'-hydroxylase [Eustoma exaltatum subsp.
           russellianum]
          Length = 510

 Score =  295 bits (756), Expect = 7e-78
 Identities = 150/289 (51%), Positives = 198/289 (68%)
 Frame = +3

Query: 3   PIMYLKLGXXXXXXXXXXXXXXXFLTTFDHKFANRPTATMKAKEAAYNGDDMVFSNYTPR 182
           P+MYLK+G               FL T D  F+NRP     A   AYN  DMVF++Y PR
Sbjct: 70  PVMYLKVGSCGLAVASTPEAAKAFLKTLDMNFSNRPP-NAGATHLAYNAQDMVFADYGPR 128

Query: 183 WKLLRRLSTIHMLGNKAFTKWADVRRSEVMHMLQAMHESSKLGKPVLVPEMLICTTTNII 362
           WKLLR+LS IH+LG KA   W +VR+ E+ +ML AM ES + G+PV+V EML     N++
Sbjct: 129 WKLLRKLSNIHILGGKALQGWEEVRKKELGYMLYAMAESGRHGQPVVVSEMLTYAMANML 188

Query: 363 GRVMLSQRVFDSVDEELGKFRNVLKELMTGAGMFNIGDFVPGIGWMDLQGVKAKLVKAHR 542
           G+VMLS+RVF S   E  +F++++ ELMT AG FNIGDF+P I WMDLQG++  + + H+
Sbjct: 189 GQVMLSKRVFGSQGSESNEFKDMVVELMTVAGYFNIGDFIPSIAWMDLQGIQGGMKRLHK 248

Query: 543 KMDDMMKRMLALHKATMKEREGCQDFIDLVLCSELRDDDGEKLSDVNIRALLSNMFTAGT 722
           K D ++ R+L  H A+  ER+G  DF+D V+ +   + +GE+L  VNI+ALL NMFTAGT
Sbjct: 249 KFDALLTRLLEEHTASAHERKGSPDFLDFVVANR-DNSEGERLHTVNIKALLLNMFTAGT 307

Query: 723 DTSTIIVEWALAELIKNPIILKRLQSEMDAIIGQERILQEDDISNLPYL 869
           DTS+ ++EWALAEL+KNPIILKR Q EMD +IG++R   E DIS LPYL
Sbjct: 308 DTSSSVIEWALAELLKNPIILKRAQEEMDGVIGRDRRFLEADISKLPYL 356


>ref|XP_002314004.1| flavonoid 3',5'-hydroxylase [Populus trichocarpa]
           gi|222850412|gb|EEE87959.1| flavonoid 3',5'-hydroxylase
           [Populus trichocarpa]
          Length = 509

 Score =  295 bits (755), Expect = 9e-78
 Identities = 146/289 (50%), Positives = 203/289 (70%)
 Frame = +3

Query: 3   PIMYLKLGXXXXXXXXXXXXXXXFLTTFDHKFANRPTATMKAKEAAYNGDDMVFSNYTPR 182
           P+MYLK+G               FL T D  F+NRP     A   AYN  DMVF++Y PR
Sbjct: 70  PVMYLKMGTCNMVVASTPDAARAFLKTLDLNFSNRPP-NAGATHLAYNAQDMVFADYGPR 128

Query: 183 WKLLRRLSTIHMLGNKAFTKWADVRRSEVMHMLQAMHESSKLGKPVLVPEMLICTTTNII 362
           WKLLR+LS +HMLG KA   WA VR SE+ HML+AM E+S+ G+PV+VPEML     N+I
Sbjct: 129 WKLLRKLSNLHMLGGKALEDWAHVRVSELGHMLRAMCEASRKGEPVVVPEMLTYAMANMI 188

Query: 363 GRVMLSQRVFDSVDEELGKFRNVLKELMTGAGMFNIGDFVPGIGWMDLQGVKAKLVKAHR 542
           G+++LS+RVF +   E  +F++++ ELMT AG+FN+GD++P + WMDLQG++  + + HR
Sbjct: 189 GQIILSRRVFVTKGSESNEFKDMVVELMTSAGLFNVGDYIPSVAWMDLQGIERGMKRLHR 248

Query: 543 KMDDMMKRMLALHKATMKEREGCQDFIDLVLCSELRDDDGEKLSDVNIRALLSNMFTAGT 722
           + D ++ +M+  H AT  ER+G  DF+D+++ ++  + DGEKLS  NI+ALL N+FTAGT
Sbjct: 249 RFDVLLTKMMEEHIATAHERKGKPDFLDVLMANQ-ENLDGEKLSFTNIKALLLNLFTAGT 307

Query: 723 DTSTIIVEWALAELIKNPIILKRLQSEMDAIIGQERILQEDDISNLPYL 869
           DTS+ I+EW+LAE++KNP ILK+ Q EMD +IG+ R L+E DI  LPYL
Sbjct: 308 DTSSSIIEWSLAEMLKNPRILKQAQDEMDQVIGRNRRLEESDIPKLPYL 356


>ref|XP_002314003.1| cytochrome P450 flavonoid 3',5'-hydroxylase [Populus trichocarpa]
           gi|222850411|gb|EEE87958.1| cytochrome P450 flavonoid
           3',5'-hydroxylase [Populus trichocarpa]
          Length = 505

 Score =  295 bits (755), Expect = 9e-78
 Identities = 146/289 (50%), Positives = 203/289 (70%)
 Frame = +3

Query: 3   PIMYLKLGXXXXXXXXXXXXXXXFLTTFDHKFANRPTATMKAKEAAYNGDDMVFSNYTPR 182
           P+MYLK+G               FL T D  F+NRP     A   AYN  DMVF++Y PR
Sbjct: 66  PVMYLKMGTCNMVVASTPDAARAFLKTLDLNFSNRPP-NAGATHLAYNAQDMVFADYGPR 124

Query: 183 WKLLRRLSTIHMLGNKAFTKWADVRRSEVMHMLQAMHESSKLGKPVLVPEMLICTTTNII 362
           WKLLR+LS +HMLG KA   WA VR SE+ HML+AM E+S+ G+PV+VPEML     N+I
Sbjct: 125 WKLLRKLSNLHMLGGKALEDWAHVRVSELGHMLRAMCEASRKGEPVVVPEMLTYAMANMI 184

Query: 363 GRVMLSQRVFDSVDEELGKFRNVLKELMTGAGMFNIGDFVPGIGWMDLQGVKAKLVKAHR 542
           G+++LS+RVF +   E  +F++++ ELMT AG+FN+GD++P + WMDLQG++  + + HR
Sbjct: 185 GQIILSRRVFVTKGSESNEFKDMVVELMTSAGLFNVGDYIPSVAWMDLQGIERGMKRLHR 244

Query: 543 KMDDMMKRMLALHKATMKEREGCQDFIDLVLCSELRDDDGEKLSDVNIRALLSNMFTAGT 722
           + D ++ +M+  H AT  ER+G  DF+D+++ ++  + DGEKLS  NI+ALL N+FTAGT
Sbjct: 245 RFDVLLTKMMEEHIATAHERKGKPDFLDVLMANQ-ENLDGEKLSFTNIKALLLNLFTAGT 303

Query: 723 DTSTIIVEWALAELIKNPIILKRLQSEMDAIIGQERILQEDDISNLPYL 869
           DTS+ I+EW+LAE++KNP ILK+ Q EMD +IG+ R L+E DI  LPYL
Sbjct: 304 DTSSSIIEWSLAEMLKNPRILKQAQDEMDQVIGRNRRLEESDIPKLPYL 352


>sp|O04790.1|C75A7_EUSGR RecName: Full=Flavonoid 3',5'-hydroxylase; Short=F3'5'H; AltName:
           Full=Cytochrome P450 75A7 gi|1785486|dbj|BAA03439.1|
           flavonoid 3',5'-hydroxylase [Eustoma grandiflorum]
           gi|50788702|dbj|BAD34460.1| flavonoid 3',5'-hydroxylase
           [Eustoma grandiflorum] gi|344178889|dbj|BAK64100.1|
           flavonoid 3',5'-hydroxylase [Eustoma exaltatum subsp.
           russellianum]
          Length = 510

 Score =  294 bits (752), Expect = 2e-77
 Identities = 149/289 (51%), Positives = 198/289 (68%)
 Frame = +3

Query: 3   PIMYLKLGXXXXXXXXXXXXXXXFLTTFDHKFANRPTATMKAKEAAYNGDDMVFSNYTPR 182
           P+MYLK+G               FL T D  F+NRP     A   AYN  DMVF++Y PR
Sbjct: 70  PVMYLKVGSCGLAVASTPEAAKAFLKTLDMNFSNRPP-NAGATHLAYNAQDMVFADYGPR 128

Query: 183 WKLLRRLSTIHMLGNKAFTKWADVRRSEVMHMLQAMHESSKLGKPVLVPEMLICTTTNII 362
           WKLLR+LS IH+LG KA   W +VR+ E+ +ML AM ES + G+PV+V EML     N++
Sbjct: 129 WKLLRKLSNIHILGGKALQGWEEVRKKELGYMLYAMAESGRHGQPVVVSEMLTYAMANML 188

Query: 363 GRVMLSQRVFDSVDEELGKFRNVLKELMTGAGMFNIGDFVPGIGWMDLQGVKAKLVKAHR 542
           G+VMLS+RVF S   E  +F++++ ELMT AG FNIGDF+P I WMDLQG++  + + H+
Sbjct: 189 GQVMLSKRVFGSQGSESNEFKDMVVELMTVAGYFNIGDFIPSIAWMDLQGIQGGMKRLHK 248

Query: 543 KMDDMMKRMLALHKATMKEREGCQDFIDLVLCSELRDDDGEKLSDVNIRALLSNMFTAGT 722
           K D ++ R+L  H A+  ER+G  DF+D V+ +   + +GE+L  VNI+ALL NMFTAGT
Sbjct: 249 KFDALLTRLLEEHTASAHERKGSPDFLDFVVANG-DNSEGERLQTVNIKALLLNMFTAGT 307

Query: 723 DTSTIIVEWALAELIKNPIILKRLQSEMDAIIGQERILQEDDISNLPYL 869
           DTS+ ++EWALAEL+KNPIIL+R Q EMD +IG++R   E DIS LPYL
Sbjct: 308 DTSSSVIEWALAELLKNPIILRRAQEEMDGVIGRDRRFLEADISKLPYL 356