BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00013019
         (2145 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002275659.2| PREDICTED: 3-epi-6-deoxocathasterone 23-mono...   597   e-168
emb|CBI35940.3| unnamed protein product [Vitis vinifera]              597   e-168
ref|XP_002329023.1| cytochrome P450 probable 6-deoxoteasterone t...   589   e-166
ref|XP_002327539.1| cytochrome P450 probable 6-deoxoteasterone t...   589   e-165
ref|XP_002527414.1| cytochrome P450, putative [Ricinus communis]...   587   e-165

>ref|XP_002275659.2| PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase-like [Vitis
            vinifera]
          Length = 478

 Score =  597 bits (1540), Expect = e-168
 Identities = 306/487 (62%), Positives = 359/487 (73%), Gaps = 4/487 (0%)
 Frame = -3

Query: 1897 MDFNNKVCMTLFFMISIIILFTFMSTRFXXXXXXXXXXXXXXXXLPSGSLGWPFFGETLE 1718
            M    +V  T  F+ +II+L+   S                   LP G+LGWP  GETLE
Sbjct: 4    MSSGERVVPTAIFLSTIILLYRNRSR----------IRSSPSSSLPLGTLGWPLIGETLE 53

Query: 1717 FISCAYSPHPESFMNKRRLLYGKVFKSHIFGSPTIVSTDAEVSRVVLQSDAKAFVPSYPK 1538
            FISCAYS  PESFM +RR +YGKVFKSHIFGSPTIVS DAEVSR VLQSD+KAFVPSYPK
Sbjct: 54   FISCAYSDRPESFMERRRRMYGKVFKSHIFGSPTIVSIDAEVSRFVLQSDSKAFVPSYPK 113

Query: 1537 SLTELMGKSSILLINGGLHRRVHGLIGAFFKSPLLKSQITTDMHKYVHHLMSGWQDNQLI 1358
            SLTELMG+SSILLING L RRVHGLIGAFFKSP LK+QIT +M  Y+   M  W+D+  I
Sbjct: 114  SLTELMGQSSILLINGSLQRRVHGLIGAFFKSPHLKAQITQEMESYIQKSMGSWRDDHPI 173

Query: 1357 FIQDETKHIVFQILVRVLIGLEPGEEMQFLKHNFQEFIAGLMSLPVKLPGCRLYKSLQAK 1178
            FIQDE K+I FQ+LV+ LI L PGEEM+FL+  FQEFI+GLMSLPV +PG RLY+SLQAK
Sbjct: 174  FIQDEAKNIAFQVLVKALISLNPGEEMEFLRKQFQEFISGLMSLPVNIPGTRLYRSLQAK 233

Query: 1177 KRMVMLVXXXXXXXXXXXXXXXXXXXXXSDHQNTPRDVIXXXXXXXXXXXXXXXXXXNMI 998
            K+MV LV                     +     P+DV+                  NMI
Sbjct: 234  KKMVQLV-----------GKIIQERRNINQPSKVPKDVLDVLLNDSSQLLTDTLISDNMI 282

Query: 997  DLMIPGEDSVPILVTLAIKYLSDCPPALKQLEDENMQLKQQKS----SLSWTDYLSLTFT 830
            DLMIPGEDSVP+LVTLAIKYLSDCP AL+QL +ENM+LK+ K+    +++W+DYLSL FT
Sbjct: 283  DLMIPGEDSVPVLVTLAIKYLSDCPAALQQLTEENMRLKRLKAERGETMTWSDYLSLPFT 342

Query: 829  QDVITETLRLGNIISGIIRMAMKDMEIKGHFIPKGWCVFTYFRSVHLDETIHENPHKFNP 650
            Q VITETLRLGN+I G++R AMKD+EIKGH IPKGWCVF YFRSVHLDE+ ++ P++FNP
Sbjct: 343  QTVITETLRLGNVIIGVMRKAMKDVEIKGHRIPKGWCVFAYFRSVHLDESQYDWPYQFNP 402

Query: 649  WRWKDKDMNSCGFTAFGGGQRLCPGLDLARLEASIFLHHLVTNFTWVAEEDQVVNFPTVR 470
            WRW+DK+++SC FT FGGGQRLCPGLDLARLEASIFLHH VT F WVAE+D +VNFPTVR
Sbjct: 403  WRWQDKNISSCSFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWVAEDDSIVNFPTVR 462

Query: 469  MKRRMPI 449
            MKRRMP+
Sbjct: 463  MKRRMPV 469


>emb|CBI35940.3| unnamed protein product [Vitis vinifera]
          Length = 475

 Score =  597 bits (1538), Expect = e-168
 Identities = 297/442 (67%), Positives = 345/442 (78%), Gaps = 4/442 (0%)
 Frame = -3

Query: 1762 PSGSLGWPFFGETLEFISCAYSPHPESFMNKRRLLYGKVFKSHIFGSPTIVSTDAEVSRV 1583
            P G+LGWP  GETLEFISCAYS  PESFM +RR +YGKVFKSHIFGSPTIVS DAEVSR 
Sbjct: 36   PLGTLGWPLIGETLEFISCAYSDRPESFMERRRRMYGKVFKSHIFGSPTIVSIDAEVSRF 95

Query: 1582 VLQSDAKAFVPSYPKSLTELMGKSSILLINGGLHRRVHGLIGAFFKSPLLKSQITTDMHK 1403
            VLQSD+KAFVPSYPKSLTELMG+SSILLING L RRVHGLIGAFFKSP LK+QIT +M  
Sbjct: 96   VLQSDSKAFVPSYPKSLTELMGQSSILLINGSLQRRVHGLIGAFFKSPHLKAQITQEMES 155

Query: 1402 YVHHLMSGWQDNQLIFIQDETKHIVFQILVRVLIGLEPGEEMQFLKHNFQEFIAGLMSLP 1223
            Y+   M  W+D+  IFIQDE K+I FQ+LV+ LI L PGEEM+FL+  FQEFI+GLMSLP
Sbjct: 156  YIQKSMGSWRDDHPIFIQDEAKNIAFQVLVKALISLNPGEEMEFLRKQFQEFISGLMSLP 215

Query: 1222 VKLPGCRLYKSLQAKKRMVMLVXXXXXXXXXXXXXXXXXXXXXSDHQNTPRDVIXXXXXX 1043
            V +PG RLY+SLQAKK+MV LV                     +     P+DV+      
Sbjct: 216  VNIPGTRLYRSLQAKKKMVQLV-----------GKIIQERRNINQPSKVPKDVLDVLLND 264

Query: 1042 XXXXXXXXXXXXNMIDLMIPGEDSVPILVTLAIKYLSDCPPALKQLEDENMQLKQQKS-- 869
                        NMIDLMIPGEDSVP+LVTLAIKYLSDCP AL+QL +ENM+LK+ K+  
Sbjct: 265  SSQLLTDTLISDNMIDLMIPGEDSVPVLVTLAIKYLSDCPAALQQLTEENMRLKRLKAER 324

Query: 868  --SLSWTDYLSLTFTQDVITETLRLGNIISGIIRMAMKDMEIKGHFIPKGWCVFTYFRSV 695
              +++W+DYLSL FTQ VITETLRLGN+I G++R AMKD+EIKGH IPKGWCVF YFRSV
Sbjct: 325  GETMTWSDYLSLPFTQTVITETLRLGNVIIGVMRKAMKDVEIKGHRIPKGWCVFAYFRSV 384

Query: 694  HLDETIHENPHKFNPWRWKDKDMNSCGFTAFGGGQRLCPGLDLARLEASIFLHHLVTNFT 515
            HLDE+ ++ P++FNPWRW+DK+++SC FT FGGGQRLCPGLDLARLEASIFLHH VT F 
Sbjct: 385  HLDESQYDWPYQFNPWRWQDKNISSCSFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFR 444

Query: 514  WVAEEDQVVNFPTVRMKRRMPI 449
            WVAE+D +VNFPTVRMKRRMP+
Sbjct: 445  WVAEDDSIVNFPTVRMKRRMPV 466


>ref|XP_002329023.1| cytochrome P450 probable 6-deoxoteasterone to 3-dehydro
            6-deoxoteasterone or teasterone to 3-dehydro teasterone
            [Populus trichocarpa] gi|222839694|gb|EEE78017.1|
            cytochrome P450 probable 6-deoxoteasterone to 3-dehydro
            6-deoxoteasterone or teasterone to 3-dehydro teasterone
            [Populus trichocarpa]
          Length = 456

 Score =  589 bits (1519), Expect = e-166
 Identities = 299/443 (67%), Positives = 339/443 (76%), Gaps = 5/443 (1%)
 Frame = -3

Query: 1762 PSGSLGWPFFGETLEFISCAYSPHPESFMNKRRLLYGKVFKSHIFGSPTIVSTDAEVSRV 1583
            P G+LGWPF GET++F+SCAYS  PESFM+KRR +YGKVFKSHIFGSPTIVSTDAEVS+ 
Sbjct: 15   PLGTLGWPFIGETIDFVSCAYSDRPESFMDKRRRMYGKVFKSHIFGSPTIVSTDAEVSKF 74

Query: 1582 VLQSDAKAFVPSYPKSLTELMGKSSILLINGGLHRRVHGLIGAFFKSPLLKSQITTDMHK 1403
            +LQSDAK FVPSYPKSLTELMGKSSILLING L RR+HGLIGAFFKSP LK+QIT DM  
Sbjct: 75   ILQSDAKVFVPSYPKSLTELMGKSSILLINGSLQRRIHGLIGAFFKSPHLKAQITRDMQS 134

Query: 1402 YVHHLMSGWQDNQLIFIQDETKHIVFQILVRVLIGLEPGEEMQFLKHNFQEFIAGLMSLP 1223
            YV   M  W+++Q IFIQDETK+I FQ+LV+ LI L+PGEEM+ LK  FQEFIAGLMSLP
Sbjct: 135  YVQESMEKWREDQPIFIQDETKNIAFQVLVKALISLDPGEEMELLKKQFQEFIAGLMSLP 194

Query: 1222 VKLPGCRLYKSLQAKKRMVMLVXXXXXXXXXXXXXXXXXXXXXSDHQNTPRDVIXXXXXX 1043
            + +PG +LY+SLQAKK+MV LV                           P+DV       
Sbjct: 195  LNIPGSQLYRSLQAKKKMVKLVQKIIQSKREGRAGIV---------SMAPKDVAEVLLND 245

Query: 1042 XXXXXXXXXXXXNMIDLMIPGEDSVPILVTLAIKYLSDCPPALKQLEDENMQLKQQKSS- 866
                        NMID+MIPGEDSVP+L+TLA+KYLSDCP AL+QL  ENM+LK  K+  
Sbjct: 246  ASEQLTDDLIADNMIDMMIPGEDSVPVLMTLAVKYLSDCPAALQQLTKENMKLKSLKAQH 305

Query: 865  ---LSWTDYLSLTFTQDVITETLRLGNIISGIIRMAMKDMEIKGHFIPKGWCVFTYFRSV 695
               L WTDYLSL FTQ VITETLR+GNII G++R AMKD EIKG+ IPKGWC F YFRSV
Sbjct: 306  GEPLCWTDYLSLPFTQTVITETLRMGNIIIGVMRKAMKDTEIKGYLIPKGWCAFAYFRSV 365

Query: 694  HLDETIHENPHKFNPWRWKDKDMNSCG-FTAFGGGQRLCPGLDLARLEASIFLHHLVTNF 518
            HLDE  +E P++FNPWRW+DKDM+  G FT FGGGQRLCPGLDLARLEASIFLHH VT F
Sbjct: 366  HLDENNYEWPYEFNPWRWQDKDMSINGSFTPFGGGQRLCPGLDLARLEASIFLHHFVTQF 425

Query: 517  TWVAEEDQVVNFPTVRMKRRMPI 449
             WVAEED VVNFPTVRMKRRMPI
Sbjct: 426  RWVAEEDTVVNFPTVRMKRRMPI 448


>ref|XP_002327539.1| cytochrome P450 probable 6-deoxoteasterone to 3-dehydro
            6-deoxoteasterone or teasterone to 3-dehydro teasterone
            [Populus trichocarpa] gi|222836093|gb|EEE74514.1|
            cytochrome P450 probable 6-deoxoteasterone to 3-dehydro
            6-deoxoteasterone or teasterone to 3-dehydro teasterone
            [Populus trichocarpa]
          Length = 459

 Score =  589 bits (1518), Expect = e-165
 Identities = 295/446 (66%), Positives = 344/446 (77%), Gaps = 8/446 (1%)
 Frame = -3

Query: 1762 PSGSLGWPFFGETLEFISCAYSPHPESFMNKRRLLYGKVFKSHIFGSPTIVSTDAEVSRV 1583
            P G+LGWPF GET++F+SCAYS  PESFM+KRR +YGKVFKSHIFGSPTIVSTDAEVS+ 
Sbjct: 20   PLGALGWPFIGETIDFVSCAYSDRPESFMDKRRRMYGKVFKSHIFGSPTIVSTDAEVSKF 79

Query: 1582 VLQSDAKAFVPSYPKSLTELMGKSSILLINGGLHRRVHGLIGAFFKSPLLKSQITTDMHK 1403
            +LQSDA+ FVPSYPKSLTELMGKSSILLING L RR+HGLIGAFFKSP LK+QIT DM  
Sbjct: 80   ILQSDARLFVPSYPKSLTELMGKSSILLINGSLQRRIHGLIGAFFKSPHLKAQITRDMQS 139

Query: 1402 YVHHLMSGWQDNQLIFIQDETKHIVFQILVRVLIGLEPGEEMQFLKHNFQEFIAGLMSLP 1223
            YV   M  W+++  IFIQDETK+I FQ+LV+ LI L+PGEEM+ LK  FQEFIAGLMSLP
Sbjct: 140  YVQESMEKWREDHPIFIQDETKNIAFQVLVKALISLDPGEEMELLKKQFQEFIAGLMSLP 199

Query: 1222 VK-LPGCRLYKSLQAKKRMVMLVXXXXXXXXXXXXXXXXXXXXXSDH---QNTPRDVIXX 1055
            +K +PG +LY+SLQAKK+MV LV                      DH      P+D++  
Sbjct: 200  LKNIPGSQLYRSLQAKKKMVKLV--------------QKIIKSKRDHGMISMVPKDLVQV 245

Query: 1054 XXXXXXXXXXXXXXXXNMIDLMIPGEDSVPILVTLAIKYLSDCPPALKQLEDENMQLKQQ 875
                            NMID+MIPGEDSVP+L+TLA+KYLSDCPPAL QL +ENM+LK  
Sbjct: 246  LLNDASEQLTDDLIADNMIDMMIPGEDSVPVLMTLAVKYLSDCPPALHQLTEENMKLKSL 305

Query: 874  KSS----LSWTDYLSLTFTQDVITETLRLGNIISGIIRMAMKDMEIKGHFIPKGWCVFTY 707
            K+     + W+DYLSL FTQ VITETLR+GNII G++R AMKD+EIKG+ IPKGWC F Y
Sbjct: 306  KAQHGEPMCWSDYLSLPFTQTVITETLRMGNIIIGVMRKAMKDIEIKGYLIPKGWCAFAY 365

Query: 706  FRSVHLDETIHENPHKFNPWRWKDKDMNSCGFTAFGGGQRLCPGLDLARLEASIFLHHLV 527
            FRSVHLDE  +E P++FNPWRW+DKDM++  FT FGGGQRLCPGLDLARLEASIFLHH V
Sbjct: 366  FRSVHLDENNYEWPYQFNPWRWQDKDMSNSSFTPFGGGQRLCPGLDLARLEASIFLHHFV 425

Query: 526  TNFTWVAEEDQVVNFPTVRMKRRMPI 449
            T F WVAE+D +VNFPTVRMKRRMPI
Sbjct: 426  TQFRWVAEDDTIVNFPTVRMKRRMPI 451


>ref|XP_002527414.1| cytochrome P450, putative [Ricinus communis]
            gi|223533224|gb|EEF34980.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 482

 Score =  587 bits (1514), Expect = e-165
 Identities = 292/442 (66%), Positives = 341/442 (77%), Gaps = 4/442 (0%)
 Frame = -3

Query: 1762 PSGSLGWPFFGETLEFISCAYSPHPESFMNKRRLLYGKVFKSHIFGSPTIVSTDAEVSRV 1583
            P G+LGWPF GETLEF+SCAYS  PESFM+KRR +YGKVFKSHIFG+PTIVSTDAEVS+ 
Sbjct: 40   PLGNLGWPFLGETLEFVSCAYSDRPESFMDKRRRMYGKVFKSHIFGTPTIVSTDAEVSKF 99

Query: 1582 VLQSDAKAFVPSYPKSLTELMGKSSILLINGGLHRRVHGLIGAFFKSPLLKSQITTDMHK 1403
            +LQ DAK FVPSYPKSLTELMGKSSILLING L +R+HGLIG+FFKSP LK+QIT DM  
Sbjct: 100  ILQGDAKMFVPSYPKSLTELMGKSSILLINGSLQKRIHGLIGSFFKSPHLKAQITRDMQV 159

Query: 1402 YVHHLMSGWQDNQLIFIQDETKHIVFQILVRVLIGLEPGEEMQFLKHNFQEFIAGLMSLP 1223
            YV   M  W+ +Q I+IQDETK+I FQ+LV+ LI L+PG+EM+ LK  FQEFI GLMSLP
Sbjct: 160  YVQESMKNWRCDQPIYIQDETKNIAFQVLVKALISLDPGQEMESLKKQFQEFICGLMSLP 219

Query: 1222 VKLPGCRLYKSLQAKKRMVMLVXXXXXXXXXXXXXXXXXXXXXSDHQNTPRDVIXXXXXX 1043
            V +PG +LY+SLQAKK+MV LV                           P+DV+      
Sbjct: 220  VNIPGTQLYRSLQAKKKMVKLVQRIIQSKRDGGI------------STVPKDVVEVLLND 267

Query: 1042 XXXXXXXXXXXXNMIDLMIPGEDSVPILVTLAIKYLSDCPPALKQLEDENMQLKQQKSS- 866
                        NMID+MIPGEDSVP+L+TLA+KYLSDCP AL+QL DENM+LK  K+  
Sbjct: 268  ANAQLTDDLIADNMIDMMIPGEDSVPVLMTLAVKYLSDCPAALQQLTDENMKLKSLKAQL 327

Query: 865  ---LSWTDYLSLTFTQDVITETLRLGNIISGIIRMAMKDMEIKGHFIPKGWCVFTYFRSV 695
               L+WTDYLSL FTQ VITETLR+GNII G++R AMKD+EIKG+ IPKGWC F YFRSV
Sbjct: 328  GEPLTWTDYLSLPFTQRVITETLRMGNIIIGVMRKAMKDIEIKGYLIPKGWCAFAYFRSV 387

Query: 694  HLDETIHENPHKFNPWRWKDKDMNSCGFTAFGGGQRLCPGLDLARLEASIFLHHLVTNFT 515
            HLDE  ++ P++FNPWRW+DKDM++  FT FGGGQRLCPGLDLARLEASIFLH+ VT F+
Sbjct: 388  HLDENHYDWPYQFNPWRWQDKDMSNSNFTPFGGGQRLCPGLDLARLEASIFLHNFVTQFS 447

Query: 514  WVAEEDQVVNFPTVRMKRRMPI 449
            WVAEED +VNFPTVRMKRRMPI
Sbjct: 448  WVAEEDTIVNFPTVRMKRRMPI 469