BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00010069
         (3022 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI33975.3| unnamed protein product [Vitis vinifera]             1102   0.0  
ref|XP_003611420.1| Vacuolar protein sorting-associated protein ...  1019   0.0  
ref|XP_002517515.1| vacuolar protein sorting-associated protein,...  1018   0.0  
ref|XP_003538761.1| PREDICTED: uncharacterized protein LOC100780...  1012   0.0  
gb|AFN89138.1| vacuolar protein sorting 13 [Mesembryanthemum cry...   975   0.0  

>emb|CBI33975.3| unnamed protein product [Vitis vinifera]
          Length = 2801

 Score = 1102 bits (2851), Expect = 0.0
 Identities = 569/1016 (56%), Positives = 718/1016 (70%), Gaps = 9/1016 (0%)
 Frame = -2

Query: 3021 WPCSIRNTNGKNHGSLAGD-ILKHDDYGQ-EEGFSVWFPIAPKGFVAVGCVVSSGREVPS 2848
            WP S    + +  GSL  D ++ +   G+ E   S+WFP AP G+VA+GCVVS GR  P 
Sbjct: 494  WPPS---ASEEISGSLGIDNVMPNPVLGEGESNCSIWFPEAPDGYVALGCVVSPGRTRPP 550

Query: 2847 LSSALCIMSSLVSPSTLKDCISLGLTEGSSENVAFWRVENSFGSFLPADPIHTNVTAKPY 2668
            LSSA CI++SLVSP  L+DCI++G    S   +AFWRV+NS  +F+P D  H ++T + Y
Sbjct: 551  LSSAFCILASLVSPCALRDCITIGSGNMSHSRLAFWRVDNSVRTFIPMDASHLHLTVRAY 610

Query: 2667 DCRPIISSYSNSSTQAARNSSSRENSLSHDRAFLPEGSAFLNSGRLFEAVASFKLIWWNQ 2488
            + R         S +A+++S     S     A   E  A  +SG   EA+ASF LIWWNQ
Sbjct: 611  ELRHFFFRLPEVSPKASKSSDQASPS-GEVHALQSERPAAASSGCHLEAIASFHLIWWNQ 669

Query: 2487 GSASRRKLSVWRPIVPPGMVYLGDIAVQGYEPPNSSIVMHDTGDETFLKAPQDFQLVGHI 2308
             S+SR+KLS+WRP+VP GMVY GDIAVQGYEPPN+ IV+HDTGD+   KAP DFQLVG I
Sbjct: 670  NSSSRKKLSIWRPVVPRGMVYFGDIAVQGYEPPNTCIVVHDTGDDELFKAPLDFQLVGQI 729

Query: 2307 KKQRGIESISFWLPQAPPGFVVLGCVASKGPPKHDDFSSLRCIRSDMVSGDQFPDESIWD 2128
            KKQRG+ESISFWLPQAPPGFV LGC+A KG PK +DFSSLRCIRSDMV+GDQF +ES+WD
Sbjct: 730  KKQRGMESISFWLPQAPPGFVSLGCIACKGTPKPNDFSSLRCIRSDMVTGDQFLEESVWD 789

Query: 2127 ASDTKVSSKPFSLWSVGNESGTFLVRNGFKKPPKRFALRLAAPNVTSGSDNTVIDAEIKT 1948
             SD K + +PFS+W+VGN+ GTF+VR+GFKKPPKRFAL+LA PN+ SGSD+TVIDAEI T
Sbjct: 790  TSDAKHTKEPFSIWAVGNDLGTFVVRSGFKKPPKRFALKLADPNIPSGSDDTVIDAEIST 849

Query: 1947 FSAAVLDDYGGLMVPLFNISLSSIGFSLHGRPDYSNSTVSFSLAAKSYNDKYDAWEPLIE 1768
            FSA + DDYGGLM+PLFNISLS IGFSLHG+PDY NSTVSFSLAA+SYNDKY+ WEPL+E
Sbjct: 850  FSAVLFDDYGGLMIPLFNISLSGIGFSLHGKPDYLNSTVSFSLAARSYNDKYETWEPLVE 909

Query: 1767 PMDGFLRYQFDQNAPGAATQLRMTSTRDLNLNISVSNANMICQAYSSWTNLSQIQEKIEN 1588
            P+DG LRY++D NAP AA+QLR+TSTRDL LN+SVSN NMI QAY+SW+NLSQ+ E    
Sbjct: 910  PVDGSLRYKYDLNAPSAASQLRLTSTRDLKLNVSVSNVNMILQAYASWSNLSQVHELYRK 969

Query: 1587 KDAVSPSYMRRSVVDVHHRKNYYIIPVNMLGQDIFIRATEIKRISDIIKLPSGDNKLVKV 1408
              AVSP+    SV+DVHH++NYYIIP N LGQDIFIRA E++ +S+II++PSGD K VKV
Sbjct: 970  GTAVSPTDDGISVIDVHHKRNYYIIPQNKLGQDIFIRAAELRGLSNIIRMPSGDMKPVKV 1029

Query: 1407 PVSKNMLDSHMKGRLGIISRSMVTMIIGDGELPVGEGMTKGPITLAIRLFLIE--PSDTL 1234
            PVSKNMLDSH+KG++    R+MVT+II + + P  EG++    T+A+ L   +  PS +L
Sbjct: 1030 PVSKNMLDSHLKGKVCRKPRTMVTIIITEAQFPRVEGLSSHQYTVAVHLAPDQCIPSGSL 1089

Query: 1233 LQQQSARTCGSIYEHSS-SGFALVNWGEAFFFKVDSLDNYMVEFIVTDLGRGEPVGFYSA 1057
            L QQSARTCGS  +HSS S    VNW E FFFK+DSLD Y VE I+TD+G G+P+GF+SA
Sbjct: 1090 LHQQSARTCGSSPDHSSDSMLETVNWNEVFFFKIDSLDYYTVELILTDMGTGDPIGFFSA 1149

Query: 1056 PLKQIAQVLHPNSTLNNFHFEMTWVELSRTN-LRECQSDAHKNH-GRVRCAVLISPRAVK 883
            PLKQIA  +      +++  E+TW+EL     +R  Q+D  K+  GR+RCA+L+SP +  
Sbjct: 1150 PLKQIAGNIQETLYSDDYLNELTWMELYAAEFMRSTQTDKSKSTCGRIRCAILLSPMSEV 1209

Query: 882  DGKQESSHSSKPGFIQISPAKEGPWTSVRLNYAAPAACWRLGCDVIASEVTVKDGNRYVS 703
            +  ++S      GFIQISP++EGPWTSVRLNYAA AACWRLG DV+ASEV+V DGN YV+
Sbjct: 1210 EKSEQSFGGRNSGFIQISPSREGPWTSVRLNYAARAACWRLGNDVVASEVSVNDGNIYVT 1269

Query: 702  IRSLVSITNNTDFVFELRLKSKTSKENIXXXXXXXXXXXXXXDQKTVGTEEFFEIEKYSP 523
            IR LVS+ N TDFV +L L  K   E++              D   + T+EFFE EKY+P
Sbjct: 1270 IRPLVSVCNKTDFVLDLCLYPKAPSESMRQLNDAMKSKGIQIDGNRLETDEFFETEKYNP 1329

Query: 522  SLGWISCSQHLPVSSQSKPESSVGGKQAISNIELPEGWEWTDDWHVDESPGCKDGGWIYA 343
            + GW+ C     V        + G  QAIS +ELP GWEW  DW +D++      GW+YA
Sbjct: 1330 TTGWVPCL----VQPNQDRSGAEGSHQAISGVELPSGWEWIGDWKLDKTSVNTADGWVYA 1385

Query: 342  PDTGHIMWPESAGLSNPANSARQRRWIRHRQYMPSNEKDQILIGLLKPGSSIPLPLSGLT 163
            P+   + WPES       N ARQRRW+R R+++  + K QI +GLLKPG ++PLPLSGLT
Sbjct: 1386 PNLESLKWPESYNPIKFVNHARQRRWVRKRKWISGDVKQQISVGLLKPGDTVPLPLSGLT 1445

Query: 162  QP--YILEFRPKNDNDQNEYSWSVVLEKHCLSDFSLELENSDEIYLSMLTESVGLL 1
            Q   Y L+ RP N N+ +EYSWS V  +    + S   +   EI +S LTES  LL
Sbjct: 1446 QSGLYYLQLRPSNLNNPDEYSWSSVAGRPGRPEDSGTPKEYSEICVSTLTESDELL 1501



 Score = 73.9 bits (180), Expect = 2e-10
 Identities = 40/116 (34%), Positives = 57/116 (49%)
 Frame = -2

Query: 2463 SVWRPIVPPGMVYLGDIAVQGYEPPNSSIVMHDTGDETFLKAPQDFQLVGHIKKQRGIES 2284
            S+WRP+ P G V +GD+A  G  PPN + V H+ G    L  P  + LV        I  
Sbjct: 2641 SIWRPVCPDGYVSIGDVARVGCHPPNVAAVYHNVGKRFAL--PVGYDLVWRNCPDDYINP 2698

Query: 2283 ISFWLPQAPPGFVVLGCVASKGPPKHDDFSSLRCIRSDMVSGDQFPDESIWDASDT 2116
            +S W P+AP GFV LGCV      + +  S   C+   +     F ++ +W A D+
Sbjct: 2699 VSIWYPRAPEGFVSLGCVVVADFIEPEP-SLAYCVAESLAEETVFEEQKVWSAPDS 2753



 Score = 60.5 bits (145), Expect = 3e-06
 Identities = 45/155 (29%), Positives = 64/155 (41%), Gaps = 19/155 (12%)
 Frame = -2

Query: 2463 SVWRPIVPPGMVYLGDIAVQGYEPPNSSIVMHDTGDETFLKAPQDFQLV------GHIKK 2302
            ++WRP  PPG    GD      +PP   +V  +T     +K P  F+L+        I  
Sbjct: 446  ALWRPRAPPGFAVFGDYLTPLDKPPTKGVVAVNTSFAK-VKRPVSFKLIWPPSASEEISG 504

Query: 2301 QRGIESI-------------SFWLPQAPPGFVVLGCVASKGPPKHDDFSSLRCIRSDMVS 2161
              GI+++             S W P+AP G+V LGCV S G  +    SS  CI + +VS
Sbjct: 505  SLGIDNVMPNPVLGEGESNCSIWFPEAPDGYVALGCVVSPGRTR-PPLSSAFCILASLVS 563

Query: 2160 GDQFPDESIWDASDTKVSSKPFSLWSVGNESGTFL 2056
                 D          +S    + W V N   TF+
Sbjct: 564  PCALRD--CITIGSGNMSHSRLAFWRVDNSVRTFI 596


>ref|XP_003611420.1| Vacuolar protein sorting-associated protein 13A [Medicago truncatula]
            gi|355512755|gb|AES94378.1| Vacuolar protein
            sorting-associated protein 13A [Medicago truncatula]
          Length = 4721

 Score = 1019 bits (2634), Expect = 0.0
 Identities = 531/1036 (51%), Positives = 688/1036 (66%), Gaps = 57/1036 (5%)
 Frame = -2

Query: 2937 EEGFSVWFPIAPKGFVAVGCVVSSGREVPSLSSALCIMSSLVSPSTLKDCISLGLTEGSS 2758
            ++  S+WFP APKG+VA+GC+V+ GR  P LSSALCI SS VS  +L+DCI +G+   SS
Sbjct: 2402 DDSCSIWFPQAPKGYVALGCIVTQGRTPPPLSSALCIPSSSVSLCSLRDCIMIGMPNTSS 2461

Query: 2757 ENVAFWRVENSFGSFLPADPIHTNVTAKPYDCRPIISSYSNSSTQAARNSSSR-----EN 2593
             +V FWRV+NSFG+FLP DP   ++ +K Y+ R I      +S+    +  S      + 
Sbjct: 2462 SSVRFWRVDNSFGTFLPVDPTTHSLMSKAYELRCIKYGSLKASSAVLNSLDSHVHPGGQQ 2521

Query: 2592 SLSHDRAFLPEGSAFLNSGRLFEAVASFKLIWWNQGSASRRKLSVWRPIVPPGMVYLGDI 2413
            SL +D+      SA  NS R  E VASF+LIWWNQG  SR++LS+WRP+VP GMVY GD+
Sbjct: 2522 SLEYDQ------SADANSNRRLEPVASFRLIWWNQGLNSRKRLSIWRPVVPTGMVYFGDV 2575

Query: 2412 AVQGYEPPNSSIVMHDTGDETFLKAPQDFQLVGHIKKQRGIESISFWLPQAPPGFVVLGC 2233
            AV+GYEPPN+ IV+HD+ DE   K P DFQLVG IKKQRG+ESISFWLPQAPPGFV LGC
Sbjct: 2576 AVKGYEPPNTCIVLHDSRDENVFKTPLDFQLVGQIKKQRGMESISFWLPQAPPGFVSLGC 2635

Query: 2232 VASKGPPKHDDFSSLRCIRSDMVSGDQFPDESIWDASDTKVSSKPFSLWSVGNESGTFLV 2053
            VA KG PK  +FS+LRC+RSD+V+GD+F +ES+WD SD K  ++PFS+W+VGNE GTF+V
Sbjct: 2636 VACKGKPKQHEFSTLRCMRSDLVAGDKFLEESVWDTSDAKHVTEPFSIWTVGNELGTFIV 2695

Query: 2052 RNGFKKPPKRFALRLAAPNVTSGSDNTVIDAEIKTFSAAVLDDYGGLMVPLFNISLSSIG 1873
            R GFK+PP+RFAL+LA  ++ SGSD T+IDA I TFS A+ DDY GLMVPLFNISLS I 
Sbjct: 2696 RGGFKRPPRRFALKLADFSLPSGSDATIIDAGIGTFSIALFDDYSGLMVPLFNISLSGIT 2755

Query: 1872 FSLHGRPDYSNSTVSFSLAAKSYNDKYDAWEPLIEPMDGFLRYQFDQNAPGAATQLRMTS 1693
            FSLHGR +Y N TV FSLAA+SYNDKY+AWEPL+EP+DGFLRYQ+D NAPGA +QLR+TS
Sbjct: 2756 FSLHGRTEYLNCTVGFSLAARSYNDKYEAWEPLVEPVDGFLRYQYDLNAPGATSQLRLTS 2815

Query: 1692 TRDLNLNISVSNANMICQAYSSWTNLSQIQEKIENKDAVSPSYMRRSVVDVHHRKNYYII 1513
            TRDLNLN+SVSN NMI QAY+SW NLS   E  +N++A SP++   S++D  H++NYYII
Sbjct: 2816 TRDLNLNVSVSNVNMIIQAYASWNNLSHAHESYQNREAFSPTFGGNSIIDAVHKRNYYII 2875

Query: 1512 PVNMLGQDIFIRATEIKRISDIIKLPSGDNKLVKVPVSKNMLDSHMKGRLGIISRSMVTM 1333
            P N LGQDIFIRATE + +  IIK+PSGD K VKVPVSK+ML+SH++G+L    R+MVT+
Sbjct: 2876 PQNKLGQDIFIRATEARGLQSIIKMPSGDMKAVKVPVSKDMLESHLRGKLCKKIRTMVTI 2935

Query: 1332 IIGDGELPVGEGMTKGPITLAIRLFLIE--PSDTLLQQQSARTCGSIYEHSSSGFALVNW 1159
            II + + P   G       +A+RL      P+D ++ QQSARTCG       S   LV W
Sbjct: 2936 IIAEAQFPRVGGSDSQQYAVAVRLSPNPSLPTDGMVHQQSARTCGR--RAHPSDLELVKW 2993

Query: 1158 GEAFFFKVDSLDNYMVEFIVTDLGRGEPVGFYSAPLKQIAQVLHPNSTLNNFHFEMTWVE 979
             E FFFKVDSLD Y +E IVTD+  G P+GF+SA L +IA+ +  +S    F  ++ W++
Sbjct: 2994 NEIFFFKVDSLDYYTLELIVTDMSEGVPIGFFSASLSEIARTIDDSSYSQAFSNKLNWID 3053

Query: 978  LSRTNLRECQ--------SDAHKNHGR-VRCAVLISPRAVKDGKQESSHS-SKPGFIQIS 829
            LS  +             SD ++   R +RCA+L+    V++  Q S++   K GFIQIS
Sbjct: 3054 LSAEDSLSMVNVVYDLPFSDVYQKKARKLRCAILMHSSEVQNSNQNSNNDVHKSGFIQIS 3113

Query: 828  PAKEGPWTSVRLNYAAPAACWRLGCDVIASEVTVKDGNRYVSIRSLVSITNNTDFVFELR 649
            P+KEGPWT+VRLNYAAPAACWRLG  V+ASE +VKDGNRYV+IRSLVS+ N TDFV +LR
Sbjct: 3114 PSKEGPWTTVRLNYAAPAACWRLGNAVVASEASVKDGNRYVNIRSLVSVRNYTDFVLDLR 3173

Query: 648  LKSKTSKENIXXXXXXXXXXXXXXDQKTVGTEEFFEIEKYSPSLGWISCS----QHLPVS 481
            L SK   E +              +   + T+EF+E EK +   GW+  S    QH    
Sbjct: 3174 LSSKIPSEKVNLLNNSSDSESIVTESSRIQTDEFYETEKLTAHSGWVRWSGYPGQHNSYK 3233

Query: 480  SQS-KPESSVGGKQAIS---------------------------------NIELPEGWEW 403
             +S + ES +   + ++                                  I+LP GWEW
Sbjct: 3234 GKSHQLESQLSALEGVTTFRFLLLQITKFSPPKLQYPVVDLLSVIDPDSPEIDLPPGWEW 3293

Query: 402  TDDWHVDESPGCKDGGWIYAPDTGHIMWPESAGLSNPANSARQRRWIRHRQYMPSNEKDQ 223
             DDWH+D        GW YAPD   + WPES      +NSARQR+W+R+R+ +  + K +
Sbjct: 3294 IDDWHLDTKSTNTSDGWTYAPDVESLRWPESVDPKVSSNSARQRKWLRNRKLIADDLKHE 3353

Query: 222  ILIGLLKPGSSIPLPLSGLTQ--PYILEFRPKNDNDQNEYSWSVVLEKHCLSDFSLELEN 49
            I +GLL+PG ++PLPLSGLTQ   Y L+ RP +  +  EYSWS V ++  LS+     E 
Sbjct: 3354 ISVGLLQPGEAVPLPLSGLTQSIQYFLQLRPGSSENPYEYSWSTVTDRPRLSEDVGNGEQ 3413

Query: 48   SDEIYLSMLTESVGLL 1
               + +S L+ES  LL
Sbjct: 3414 CSNLCVSALSESEELL 3429



 Score = 78.2 bits (191), Expect = 1e-11
 Identities = 42/118 (35%), Positives = 60/118 (50%), Gaps = 2/118 (1%)
 Frame = -2

Query: 2463 SVWRPIVPPGMVYLGDIAVQGYEPPNSSIVMHDTGDETFLKAPQDFQLVGHIKKQRGIES 2284
            S+WRP+ P G  ++GDI+  G  PPN + V      + F   P  + LV     +  +  
Sbjct: 4564 SIWRPVCPDGYTFIGDISRVGVHPPNVAAVYRKI--DGFFALPMGYDLVWRNCLEDYVSP 4621

Query: 2283 ISFWLPQAPPGFVVLGCVASKG--PPKHDDFSSLRCIRSDMVSGDQFPDESIWDASDT 2116
            +S W P+AP GFV  GCVA  G   P+ D    + CI   +V   QF D+ +W A D+
Sbjct: 4622 VSIWHPRAPDGFVSPGCVAVAGYMEPEPD---LVHCIAESLVEETQFEDQKVWSAPDS 4676



 Score = 69.7 bits (169), Expect = 4e-09
 Identities = 53/150 (35%), Positives = 66/150 (44%), Gaps = 16/150 (10%)
 Frame = -2

Query: 2457 WRPIVPPGMVYLGDIAVQGYEPPNSSIVMHDTGDETFLKAPQDFQLVGHIKKQRGIE--- 2287
            WRP  PPG   LGD      +PP   ++  +T   T +K P  F+L+       G E   
Sbjct: 2334 WRPHAPPGFAVLGDYLTPLDKPPTKGVLAVNTNSIT-VKRPIHFRLIWPPLGTSGEEMDN 2392

Query: 2286 -----------SISFWLPQAPPGFVVLGCVASKG--PPKHDDFSSLRCIRSDMVSGDQFP 2146
                       S S W PQAP G+V LGC+ ++G  PP     SS  CI S  VS     
Sbjct: 2393 SDLSWKTEVDDSCSIWFPQAPKGYVALGCIVTQGRTPP---PLSSALCIPSSSVSLCSLR 2449

Query: 2145 DESIWDASDTKVSSKPFSLWSVGNESGTFL 2056
            D  +    +T  SS  F  W V N  GTFL
Sbjct: 2450 DCIMIGMPNTSSSSVRF--WRVDNSFGTFL 2477


>ref|XP_002517515.1| vacuolar protein sorting-associated protein, putative [Ricinus
            communis] gi|223543526|gb|EEF45057.1| vacuolar protein
            sorting-associated protein, putative [Ricinus communis]
          Length = 4226

 Score = 1018 bits (2632), Expect = 0.0
 Identities = 535/984 (54%), Positives = 674/984 (68%), Gaps = 9/984 (0%)
 Frame = -2

Query: 2925 SVWFPIAPKGFVAVGCVVSSGREVPSLSSALCIMSSLVSPSTLKDCISLGLTEGSSENVA 2746
            S+WFP APKG+VA+GCVVS+GR  P L            PSTL                A
Sbjct: 2046 SLWFPEAPKGYVALGCVVSTGRTQPHLY-----------PSTL----------------A 2078

Query: 2745 FWRVENSFGSFLPADPIHTNVTAKPYDCRPIISSYSNSSTQAARNSSSRENSLSHDRAFL 2566
            FWRVENSFG+FLPADP   ++    Y+ R I       S++ +  S  +  S   D    
Sbjct: 2079 FWRVENSFGTFLPADPKTLSLIGGAYELRHIKYGLPEFSSRTSEISDLQTLSGDVDE-LQ 2137

Query: 2565 PEGSAFLNSGRLFEAVASFKLIWWNQGSASRRKLSVWRPIVPPGMVYLGDIAVQGYEPPN 2386
             + S  LNSGR FEAVASF+LIWWN+ S+SR+KLS+WRP+V  GMVY GDIAV+GYEPPN
Sbjct: 2138 SKNSTSLNSGRHFEAVASFQLIWWNRASSSRKKLSIWRPVVAHGMVYFGDIAVKGYEPPN 2197

Query: 2385 SSIVMHDTGDETFLKAPQDFQLVGHIKKQRGIESISFWLPQAPPGFVVLGCVASKGPPKH 2206
            + IV+HDTGD+   KAP D+QLVG IKKQRG++SISFW+PQAPPGFV LGCVA KG PK 
Sbjct: 2198 TCIVLHDTGDQDLFKAPLDYQLVGQIKKQRGMDSISFWMPQAPPGFVSLGCVACKGSPKL 2257

Query: 2205 DDFSSLRCIRSDMVSGDQFPDESIWDASDTKVSSKPFSLWSVGNESGTFLVRNGFKKPPK 2026
             DFS LRC+RSDMV+GDQF +ES+WD S+ K + + FS+W+ GNE GTF+VR+GFK+PP+
Sbjct: 2258 YDFSKLRCMRSDMVAGDQFLEESVWDTSEAKSTREQFSIWTAGNELGTFIVRSGFKRPPR 2317

Query: 2025 RFALRLAAPNVTSGSDNTVIDAEIKTFSAAVLDDYGGLMVPLFNISLSSIGFSLHGRPDY 1846
            RFAL LA P++ SGSD+TVIDAEI TFS A+ DDYGGLMVPLFNISLS IGF+LHGR  Y
Sbjct: 2318 RFALNLADPSLPSGSDDTVIDAEIGTFSTAIFDDYGGLMVPLFNISLSGIGFNLHGRTGY 2377

Query: 1845 SNSTVSFSLAAKSYNDKYDAWEPLIEPMDGFLRYQFDQNAPGAATQLRMTSTRDLNLNIS 1666
             NSTVSFSLAA+SYNDKY++WEPL+EP+DGF+RYQ+D NAPGAA+QLR+TSTR+LNLN++
Sbjct: 2378 LNSTVSFSLAARSYNDKYESWEPLVEPVDGFVRYQYDLNAPGAASQLRLTSTRELNLNVT 2437

Query: 1665 VSNANMICQAYSSWTNLSQIQEKIENKDAVSPSYMRRSVVDVHHRKNYYIIPVNMLGQDI 1486
            VSNANMI QAY+SW NLS + E  +N+D     Y  RSV+DVH ++NY+I+P N LGQDI
Sbjct: 2438 VSNANMIIQAYASWNNLSHVHEYYKNRDEFPSIYGARSVIDVHQKRNYFIVPQNKLGQDI 2497

Query: 1485 FIRATEIKRISDIIKLPSGDNKLVKVPVSKNMLDSHMKGRLGIISRSMVTMIIGDGELPV 1306
            FIRATE+   S+II++PSGD   +KVPVSKNML+SH+KG+L    R MVT+II D + P 
Sbjct: 2498 FIRATEMLGRSNIIRMPSGDILPLKVPVSKNMLESHLKGKLCAKVRKMVTVIIVDAQFPR 2557

Query: 1305 GEGMTKGPITLAIRLF--LIEPSDTLLQQQSARTCGSIYEHSSSGFALVNWGEAFFFKVD 1132
              G+T    T+AIRL    +   ++L  QQSART GSI   SSS   LVNW E FFFKVD
Sbjct: 2558 DGGLTSNFYTVAIRLTPNQVVGGESLYHQQSARTSGSISNSSSSELELVNWNEIFFFKVD 2617

Query: 1131 SLDNYMVEFIVTDLGRGEPVGFYSAPLKQIAQVLHPNSTLNNFHFEMTWVELSRTNLREC 952
              DNY++E IVTD+G+G PVGF SAPL QIA  +  + T +++   +TW++L+    R  
Sbjct: 2618 CPDNYLLELIVTDMGKGGPVGFSSAPLNQIAVKIQDSFTQSDYLNYLTWIDLAPAKSRTA 2677

Query: 951  Q--SDAHKNHGRVRCAVLISPRAVKDGKQE-SSHSSKPGFIQISPAKEGPWTSVRLNYAA 781
                +  K  GR+RC+V +SP +  + + E      KPGFIQISP  EGPWT+VRLNYAA
Sbjct: 2678 NLGEEHSKASGRIRCSVFLSPGSEAEDRYEYFVGDRKPGFIQISPGMEGPWTTVRLNYAA 2737

Query: 780  PAACWRLGCDVIASEVTVKDGNRYVSIRSLVSITNNTDFVFELRLKSKTSKENIXXXXXX 601
            PAACWRLG DV+ASEV+VKDGNR V+IRSLVS+ N+TDF+ +L L SK S +        
Sbjct: 2738 PAACWRLGNDVVASEVSVKDGNRNVTIRSLVSVRNSTDFILDLHLVSKASSD-------- 2789

Query: 600  XXXXXXXXDQKTVGTEEFFEIEKYSPSLGWISCSQHLPVSSQSKPESSVGGKQAISNIEL 421
                          T+EFFE E Y P+ GW+ CS     S         G  +A+  +EL
Sbjct: 2790 ASKSGELHSDGRTQTDEFFETEIYKPNAGWVGCSNLSDAS---------GCHEAVFGVEL 2840

Query: 420  PEGWEWTDDWHVDESPGCKDGGWIYAPDTGHIMWPESAGLSNPANSARQRRWIRHRQYMP 241
            P GWEW DDWH+D S      GW+++PD   + WPES       N ARQRRWIR+R+ + 
Sbjct: 2841 PSGWEWIDDWHLDTSSVNTSEGWVHSPDAERLKWPESFDPMKFVNHARQRRWIRNRKQIS 2900

Query: 240  SNEKDQILIGLLKPGSSIPLPLSGLTQ--PYILEFRPKNDNDQNEYSWSVVLEKHCLSDF 67
               K +I +G +KPG ++PLPLSG+TQ   YIL+ RP + N  + +SWS V+E+      
Sbjct: 2901 GEVKQEISVGSVKPGDTLPLPLSGITQFGMYILQLRPSSHNTSDGHSWSSVVER---PGQ 2957

Query: 66   SLELENS--DEIYLSMLTESVGLL 1
            ++E  NS    I +S LTE   LL
Sbjct: 2958 TVENGNSKGSGICISNLTEREELL 2981



 Score = 72.4 bits (176), Expect = 7e-10
 Identities = 49/163 (30%), Positives = 70/163 (42%)
 Frame = -2

Query: 2604 SRENSLSHDRAFLPEGSAFLNSGRLFEAVASFKLIWWNQGSASRRKLSVWRPIVPPGMVY 2425
            S  +S S +  F+  G  FL              +  NQ        S+WRPI P G + 
Sbjct: 4019 SSSSSTSAEEKFVKHGMNFLKIWSSERESKGRCKLCKNQVVEDDSICSIWRPICPNGYIS 4078

Query: 2424 LGDIAVQGYEPPNSSIVMHDTGDETFLKAPQDFQLVGHIKKQRGIESISFWLPQAPPGFV 2245
            +GDIA  G  PPN + +      +     P  + LV           +S W P+AP GFV
Sbjct: 4079 IGDIAHVGSHPPNVAALYRKI--DGLFALPMGYDLVWRNCSDDYKAPVSIWHPRAPEGFV 4136

Query: 2244 VLGCVASKGPPKHDDFSSLRCIRSDMVSGDQFPDESIWDASDT 2116
              GCVA  G  + +  S +RC+    V   +F ++ IW A D+
Sbjct: 4137 SPGCVAVAGFEEPEP-SLVRCVAESQVEQTEFEEQKIWSAPDS 4178


>ref|XP_003538761.1| PREDICTED: uncharacterized protein LOC100780088 [Glycine max]
          Length = 4246

 Score = 1012 bits (2616), Expect = 0.0
 Identities = 522/954 (54%), Positives = 663/954 (69%), Gaps = 7/954 (0%)
 Frame = -2

Query: 2925 SVWFPIAPKGFVAVGCVVSSGREVPSLSSALCIMSSLVSPSTLKDCISLGLTEGSSENVA 2746
            S+WFP APKG+VA+GC+V+ G+  P LSS+ CI S    PS+                VA
Sbjct: 2051 SIWFPEAPKGYVALGCIVTHGKTPPPLSSSFCIPS----PSS----------------VA 2090

Query: 2745 FWRVENSFGSFLPADPIHTNVTAKPYDCRPIISSYSNSSTQAARNSSSRENSLSHDRAFL 2566
            FWRV+NS G+FLP DP+  ++  K Y+ R I   +   S+ A  +  S   S  H +A  
Sbjct: 2091 FWRVDNSVGTFLPVDPVSLSLMGKAYELRCIKYDFLKPSSAALSSLDSHAPSGGH-QALQ 2149

Query: 2565 PEGSAFLNSGRLFEAVASFKLIWWNQGSASRRKLSVWRPIVPPGMVYLGDIAVQGYEPPN 2386
            P+ S   NS R  E VASF+L+WWNQGS SR++LS+WRP+VP GMVY GDIAV+G+EPPN
Sbjct: 2150 PDQSVGANSNRRCEPVASFELVWWNQGSNSRKRLSIWRPVVPMGMVYFGDIAVKGFEPPN 2209

Query: 2385 SSIVMHDTGDETFLKAPQDFQLVGHIKKQRGIESISFWLPQAPPGFVVLGCVASKGPPKH 2206
            + IV+HD+ DE   K P DFQLVG IKKQRG+ES+SFWLPQAPPGFV LGCV  KG PK 
Sbjct: 2210 TCIVVHDSRDENIFKTPLDFQLVGQIKKQRGMESMSFWLPQAPPGFVSLGCVVCKGKPKQ 2269

Query: 2205 DDFSSLRCIRSDMVSGDQFPDESIWDASDTKVSSKPFSLWSVGNESGTFLVRNGFKKPPK 2026
            +DFS+LRC+RSD+V+GD+F +ES+WD SD K  ++PFS+W+VGNE GTF+VR GFK+PP+
Sbjct: 2270 NDFSTLRCMRSDLVAGDKFLEESVWDTSDAKHVTEPFSIWAVGNELGTFIVRGGFKRPPR 2329

Query: 2025 RFALRLAAPNVTSGSDNTVIDAEIKTFSAAVLDDYGGLMVPLFNISLSSIGFSLHGRPDY 1846
            RFAL+LA  NV SGSD TVIDA I TFS A+ DDY GLMVPLFNISLS I FSLHGR  Y
Sbjct: 2330 RFALKLADSNVPSGSDATVIDAGIGTFSMALFDDYSGLMVPLFNISLSGITFSLHGRTGY 2389

Query: 1845 SNSTVSFSLAAKSYNDKYDAWEPLIEPMDGFLRYQFDQNAPGAATQLRMTSTRDLNLNIS 1666
             N TV FSLAA+SYNDKY+AWEPL+EP+DGFLRYQ+D NA  A +QLR+TSTRDLNLN+S
Sbjct: 2390 LNCTVGFSLAARSYNDKYEAWEPLVEPVDGFLRYQYDLNALAATSQLRLTSTRDLNLNVS 2449

Query: 1665 VSNANMICQAYSSWTNLSQIQEKIENKDAVSPSYMRRSVVDVHHRKNYYIIPVNMLGQDI 1486
            VSNANMI QAY+SW NLS   E  +N DA SP+Y   S++D  H+KNYYIIP N LGQDI
Sbjct: 2450 VSNANMIIQAYASWNNLSHAHECYKNIDAFSPTYGGNSIIDTLHKKNYYIIPQNKLGQDI 2509

Query: 1485 FIRATEIKRISDIIKLPSGDNKLVKVPVSKNMLDSHMKGRLGIISRSMVTMIIGDGELPV 1306
            FIR TE + + +II++PSGD K VKVPVSKNML+SH+KG+L    R+MVT+II + + P 
Sbjct: 2510 FIRVTEARGLQNIIRMPSGDMKAVKVPVSKNMLESHLKGKLCRKIRTMVTIIIAEAQFPQ 2569

Query: 1305 GEGMTKGPITLAIRLFLIE--PSDTLLQQQSARTCGSIYEH-SSSGFALVNWGEAFFFKV 1135
             EG      T+A+RL+  +  PSD+ + QQSART G    H   S   LV W E FFFKV
Sbjct: 2570 VEGSDSQQYTVAVRLYSNQSLPSDSSVYQQSARTRGRRAHHLLPSDLELVKWNEIFFFKV 2629

Query: 1134 DSLDNYMVEFIVTDLGRGEPVGFYSAPLKQIAQVLHPNSTLNNFHFEMTWVELSRTNLRE 955
            DSLDN+ +E I+TD+G+G PVGF+SA L ++A+ +   S   NF  ++ W++LS  N   
Sbjct: 2630 DSLDNHSLELILTDMGKGVPVGFFSASLNEMAKTIEDCSYTQNFANKLNWIDLSAENSMV 2689

Query: 954  CQSDAHKNHGRVRCAVLISPRAVKDGKQESSHSS-KPGFIQISPAKEGPWTSVRLNYAAP 778
                  K   +++CA+L+    V+   Q S++ + K GFIQISP+KEGPWT+VRLNYAAP
Sbjct: 2690 NFDAFSKKPCKLQCAILVHNSEVETNNQLSNYDAHKSGFIQISPSKEGPWTTVRLNYAAP 2749

Query: 777  AACWRLGCDVIASEVTVKDGNRYVSIRSLVSITNNTDFVFELRLKSKTSKENIXXXXXXX 598
            AACWRLG  V+ASE +VKDGNRYV+IRSLVS+ NNTDFV +L L SK+  E         
Sbjct: 2750 AACWRLGNAVVASEASVKDGNRYVNIRSLVSVRNNTDFVLDLCLTSKSLSEKGNLLKNSI 2809

Query: 597  XXXXXXXDQKTVGTEEFFEIEKYSPSLGWISCSQHLPVSSQSKPESSVGGKQAISNIELP 418
                   +   + T+EFFE EK +P +GW+ CS +    S+++        Q    I+LP
Sbjct: 2810 NSESIHTESYRIQTDEFFETEKLTPHIGWVHCSGY----SENQMSDRGKSHQVFPGIDLP 2865

Query: 417  EGWEWTDDWHVD-ESPGCKDGGWIYAPDTGHIMWPESAGLSNPANSARQRRWIRHRQYMP 241
             GWEW DDWH+D +SP   D GWIYAPD   + WPES       NSARQRRW+R+R+ + 
Sbjct: 2866 PGWEWIDDWHLDTKSPNTSD-GWIYAPDVESLRWPESFDPKVSLNSARQRRWLRNRKLIA 2924

Query: 240  SNEKDQILIGLLKPGSSIPLPLSGLTQ--PYILEFRPKNDNDQNEYSWSVVLEK 85
             + K +I +G L+PG + PLPLSGLTQ   Y L+ RP  ++   EYSWS V+++
Sbjct: 2925 EDLKHEISVGQLQPGETAPLPLSGLTQSVQYFLQLRPSENS--CEYSWSSVVDR 2976



 Score = 77.8 bits (190), Expect = 2e-11
 Identities = 45/127 (35%), Positives = 63/127 (49%), Gaps = 2/127 (1%)
 Frame = -2

Query: 2490 QGSASRRKLSVWRPIVPPGMVYLGDIAVQGYEPPNSSIVMHDTGDETFLKAPQDFQLVGH 2311
            Q S   R  S+WRP+ P G +Y+GDIA  G  PPN + V      + F   P  + LV  
Sbjct: 4080 QISQDGRICSIWRPVCPVGYIYIGDIARVGIHPPNVAAVYRKI--DGFFALPMGYDLVWR 4137

Query: 2310 IKKQRGIESISFWLPQAPPGFVVLGCVASKG--PPKHDDFSSLRCIRSDMVSGDQFPDES 2137
               +  +  +S W P+AP GFV  GCVA  G   P+ D    + CI   +V   +F +  
Sbjct: 4138 NCPEDYVTPLSIWHPRAPDGFVAPGCVAIAGYLEPEPD---LVYCIAESLVEETEFEELK 4194

Query: 2136 IWDASDT 2116
            +W A D+
Sbjct: 4195 VWSAPDS 4201


>gb|AFN89138.1| vacuolar protein sorting 13 [Mesembryanthemum crystallinum]
          Length = 3718

 Score =  975 bits (2521), Expect = 0.0
 Identities = 509/969 (52%), Positives = 663/969 (68%), Gaps = 14/969 (1%)
 Frame = -2

Query: 2925 SVWFPIAPKGFVAVGCVVSSGREVPSLSSALCIMSSLVSPSTLKDCISLGLTEGSSENVA 2746
            S+WFP+APKG++A+GCVVSSG   P+LSS+ CI++SLVS   ++DC+ +G +   S  +A
Sbjct: 2082 SIWFPVAPKGYIALGCVVSSGTAPPALSSSFCILASLVSSCPVRDCVMIGASNEHSAAMA 2141

Query: 2745 FWRVENSFGSFLPADPIHTNVTAKPYDCRPIISSYSNSSTQAARNSSSRENSLSHDRAFL 2566
            FWRV+N  G+FLP D    N+    YD RPI    S  S +   +SS    S SH+   L
Sbjct: 2142 FWRVDNCIGTFLPTDLTSKNLIRGAYDLRPIFFRLSEFS-KGVSSSSGSHVSPSHEH--L 2198

Query: 2565 PEGSAFLNSGRLFEAVASFKLIWWNQGSASRRKLSVWRPIVPPGMVYLGDIAVQGYEPPN 2386
            P  SA  NSGR  EAVASF L+WWNQ S SR+KLS+WRPIVP GMVY GDIAV+GYEPPN
Sbjct: 2199 PAQSATANSGRRLEAVASFHLVWWNQSSTSRKKLSIWRPIVPQGMVYFGDIAVKGYEPPN 2258

Query: 2385 SSIVMHDTGDETFLKAPQDFQLVGHIKKQRGIESISFWLPQAPPGFVVLGCVASKGPPKH 2206
            + +V+ D GDE F K P DFQ+VG IKK RG+E +SFWLPQAPPG+V LGC+A KG PK 
Sbjct: 2259 TCVVVEDIGDELF-KEPTDFQMVGKIKKHRGMEPVSFWLPQAPPGYVPLGCIACKGSPKE 2317

Query: 2205 DDFSSLRCIRSDMVSGDQFPDESIWDASDTKVSSKPFSLWSVGNESGTFLVRNGFKKPPK 2026
            ++F SLRCIRSDMV+GDQF DES+WD  D  +   PFS      E   F  +   KKP K
Sbjct: 2318 NEFRSLRCIRSDMVTGDQFSDESVWDTYDAGLKIGPFSYMDSCGEWEPFGPKCQ-KKPSK 2376

Query: 2025 RFALRLAAPNVTSGSDNTVIDAEIKTFSAAVLDDYGGLMVPLFNISLSSIGFSLHGRPDY 1846
            RFA++LA  +VT G ++TVIDAEI TFSAA  DD+GGLMVPLFN+S+S IGF+LHGRPDY
Sbjct: 2377 RFAVKLADKSVTGGPEDTVIDAEISTFSAACFDDFGGLMVPLFNVSVSGIGFTLHGRPDY 2436

Query: 1845 SNSTVSFSLAAKSYNDKYDAWEPLIEPMDGFLRYQFDQNAPGAATQLRMTSTRDLNLNIS 1666
             NSTVSFSLAA+SYNDKY++WEP++E +DGFLRYQ+D N+PGA +QLR+TST+DLNLN+S
Sbjct: 2437 LNSTVSFSLAARSYNDKYESWEPVVEAVDGFLRYQYDLNSPGAESQLRLTSTKDLNLNVS 2496

Query: 1665 VSNANMICQAYSSWTNLSQIQEKIENKDAVSPSYMRRSVVDVHHRKNYYIIPVNMLGQDI 1486
             SNANMI QAY+SW NL+++ +    K+AVSP+     + DVH++++Y+IIP N LGQDI
Sbjct: 2497 SSNANMILQAYASWNNLNEVHDSYGRKEAVSPTSKGSPIDDVHNKRSYFIIPQNKLGQDI 2556

Query: 1485 FIRATEIKRISDIIKLPSGDNKLVKVPVSKNMLDSHMKGRLGIISRSMVTMIIGDGELPV 1306
            FIRATE + +S +I++PSGD K +KVPVSKNM+DSH++G +     +MV++II + +   
Sbjct: 2557 FIRATEARGLSRVIRMPSGDMKPLKVPVSKNMMDSHLRGNVEQKIHAMVSLIIAEAQFQR 2616

Query: 1305 GEGMTKGPITLAIRLFLIEP---SDTLLQQQSARTCGSIYE-HSSSGFALVNWGEAFFFK 1138
             +G++     +A+RL   EP     TLL QQSARTCG   E  SSSG   V W E FFFK
Sbjct: 2617 VQGLSSRQYAVAVRLSQ-EPMLSDGTLLNQQSARTCGCSSEFSSSSGLECVKWNEVFFFK 2675

Query: 1137 VDSLDNYMVEFIVTDLGRGEPVGFYSAPLKQIAQVLHPNSTLNNFHFEMTWVELSRTNLR 958
            VD  ++Y VE IVTD+G+G+PVGF+SAPLK I  V   ++  +++     W++LS     
Sbjct: 2676 VDCPESYRVELIVTDIGKGDPVGFFSAPLKHI--VALESAYSHDYVNGWNWIDLSPPE-S 2732

Query: 957  ECQSDAHK---NHGRVRCAVLISPR-AVKDGKQESSHSSKPGFIQISPAKEGPWTSVRLN 790
            +  S+A     + G+++ AV++S +  V++ KQ      K GFIQISP +EGPWT+VRLN
Sbjct: 2733 KTMSEAENFKGSQGKLKLAVILSSKLQVEESKQSFIGDKKNGFIQISPTREGPWTTVRLN 2792

Query: 789  YAAPAACWRLGCDVIASEVTVKDGNRYVSIRSLVSITNNTDFVFELRLKSKTSKENIXXX 610
            YA PAACWRLG DV+ASEV+V+DGNRYV+IRSLVS+ N TDF  +L LK K   E+    
Sbjct: 2793 YATPAACWRLGSDVVASEVSVQDGNRYVNIRSLVSVRNETDFTLDLCLKGKALSESKKLL 2852

Query: 609  XXXXXXXXXXXDQKTVGTEEFFEIEKYSPSLGWISCSQHLPVSSQSKPES--SVGG--KQ 442
                       + + + T EF EIEK+ P   W+ CS         KP +  SV G   +
Sbjct: 2853 NDARTSEKSKMNGERIETVEFLEIEKHLPDGRWVCCS--------GKPSNGRSVTGMPDK 2904

Query: 441  AISNIELPEGWEWTDDWHVDESPGCKDGGWIYAPDTGHIMWPESAGLSNPANSARQRRWI 262
             I+ IE   GWEW DDWHVDE+      GW YAPD   + W ES   ++  N  RQRRW+
Sbjct: 2905 EIAEIESVTGWEWVDDWHVDEASVGSTDGWDYAPDQQILKWSESCDAASSVNHVRQRRWV 2964

Query: 261  RHRQYMPSNEKDQILIGLLKPGSSIPLPLSGLTQ--PYILEFRPKNDNDQNEYSWSVVLE 88
            R+R+ + S+    + +GLLKPG S+PLPLS LTQ  PY+L+ RP N    +EY+WS +++
Sbjct: 2965 RNRRQISSDSWQHVSVGLLKPGDSVPLPLSCLTQAGPYVLQLRPLNFGSSDEYAWSKLVD 3024

Query: 87   KHCLSDFSL 61
            K   S  S+
Sbjct: 3025 KPVESQSSV 3033



 Score = 65.9 bits (159), Expect = 6e-08
 Identities = 56/190 (29%), Positives = 80/190 (42%), Gaps = 27/190 (14%)
 Frame = -2

Query: 2457 WRPIVPPGMVYLGDIAVQGYEPPNSSIVMHDTGDETFLKAPQDFQLVGHIKKQRGIE--- 2287
            WRP  PPG   LGD      +PP  +++  +  +   +K P+ F+LV  +     +    
Sbjct: 2008 WRPRAPPGFAVLGDYLTPMDKPPTKAVLAVNM-NLVKIKKPESFKLVWPLIASTDVSDSE 2066

Query: 2286 -------------SISFWLPQAPPGFVVLGCVASKG--PPKHDDFSSLRCIRSDMVSGDQ 2152
                         S S W P AP G++ LGCV S G  PP     SS  CI + +VS   
Sbjct: 2067 TTSRMPDIVQRDASCSIWFPVAPKGYIALGCVVSSGTAPPA---LSSSFCILASLVSSCP 2123

Query: 2151 FPDESIWDASDTKVSSKPFSLWSVGNESGTF---------LVRNGFKKPPKRFALRLAAP 1999
              D  +  AS+   ++  F  W V N  GTF         L+R  +   P  F L   + 
Sbjct: 2124 VRDCVMIGASNEHSAAMAF--WRVDNCIGTFLPTDLTSKNLIRGAYDLRPIFFRLSEFSK 2181

Query: 1998 NVTSGSDNTV 1969
             V+S S + V
Sbjct: 2182 GVSSSSGSHV 2191