BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00009874
         (649 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002319761.1| flavonoid 3'-hydroxylase [Populus trichocarp...   303   2e-80
gb|AFC62055.1| flavonoid 3' hydroxylase [Prunus persica]              302   3e-80
dbj|BAH22519.1| flavonoid 3' hydroxylase [Tricyrtis hirta]            301   5e-80
gb|ADZ54783.1| flavonoid 3'-monooxygenase [Prunus avium]              301   7e-80
gb|ADE80941.1| flavonoid 3'-hydroxylase [Epimedium sagittatum]        300   1e-79

>ref|XP_002319761.1| flavonoid 3'-hydroxylase [Populus trichocarpa]
           gi|222858137|gb|EEE95684.1| flavonoid 3'-hydroxylase
           [Populus trichocarpa]
          Length = 521

 Score =  303 bits (776), Expect = 2e-80
 Identities = 151/223 (67%), Positives = 183/223 (82%), Gaps = 8/223 (3%)
 Frame = +3

Query: 3   LNKIMEEHRMESG------REGGDLLTVLLGLKE--DVNGDDEAKITDTNIKALLLNLFT 158
           L  I+EEH+  S        +  DLL+ L+ LKE  DV+G+ E K+TDT IKALLLN+FT
Sbjct: 252 LTNIVEEHKTSSSTASVRSEKHTDLLSTLIALKEQQDVDGE-EGKLTDTEIKALLLNMFT 310

Query: 159 AGTDTSASTVEWAMAELIRHPDILVAVQAEIDAVTGRSRLVSEPDLAKLPLLRAIIKETF 338
           AGTDTS+STVEWA+AELIRHPDIL  V+ E+D+V GR RLV+E DLA+L  L+A++KETF
Sbjct: 311 AGTDTSSSTVEWAIAELIRHPDILAQVKQELDSVVGRDRLVTELDLAQLTYLQAVVKETF 370

Query: 339 RLHPSTPLSIPRTAAEPCEIGGFHVPSGATLLVNVWAIARDPEVWSDPLEFKPSRFLPGG 518
           RLHPSTPLS+PR AAE CEIGG+H+P G+T+LVNVWAIARDP+VW+ PLEF+P RFLPGG
Sbjct: 371 RLHPSTPLSLPRIAAESCEIGGYHIPKGSTVLVNVWAIARDPDVWTKPLEFRPERFLPGG 430

Query: 519 SHADVDLKGAHFELIPFGAGRRICAGLSLGLRMVTLMVASLVH 647
             ADVD+KG  FELIPFGAGRRICAG+SLGLRMV L+ A+L+H
Sbjct: 431 DKADVDVKGNDFELIPFGAGRRICAGMSLGLRMVQLLTATLIH 473


>gb|AFC62055.1| flavonoid 3' hydroxylase [Prunus persica]
          Length = 510

 Score =  302 bits (774), Expect = 3e-80
 Identities = 148/215 (68%), Positives = 175/215 (81%)
 Frame = +3

Query: 3   LNKIMEEHRMESGREGGDLLTVLLGLKEDVNGDDEAKITDTNIKALLLNLFTAGTDTSAS 182
           L  I+EEH+  SG + GD+LT LL LKED +G+  AK+TDT IKALLLN+FTAGTDTS+S
Sbjct: 248 LTAIVEEHKKSSGGKHGDMLTTLLSLKEDADGEG-AKLTDTEIKALLLNMFTAGTDTSSS 306

Query: 183 TVEWAMAELIRHPDILVAVQAEIDAVTGRSRLVSEPDLAKLPLLRAIIKETFRLHPSTPL 362
           TVEWA+AEL+RHP IL  VQ E+D V GR +LV+E DL  L  L+A+IKETFRLHPSTPL
Sbjct: 307 TVEWAIAELLRHPKILAQVQQELDQVVGRDQLVTELDLPNLTYLQAVIKETFRLHPSTPL 366

Query: 363 SIPRTAAEPCEIGGFHVPSGATLLVNVWAIARDPEVWSDPLEFKPSRFLPGGSHADVDLK 542
           S+PR A+E CEI  FH+P GATLLVNVWAI+RDPE W +PLEF+P RFLPGG    VD++
Sbjct: 367 SLPRMASESCEINSFHIPKGATLLVNVWAISRDPEQWKEPLEFRPERFLPGGEKPHVDVR 426

Query: 543 GAHFELIPFGAGRRICAGLSLGLRMVTLMVASLVH 647
           G  FE+IPFGAGRRICAG+SLGLRMV LM A+LVH
Sbjct: 427 GNDFEVIPFGAGRRICAGMSLGLRMVHLMAATLVH 461


>dbj|BAH22519.1| flavonoid 3' hydroxylase [Tricyrtis hirta]
          Length = 505

 Score =  301 bits (772), Expect = 5e-80
 Identities = 148/215 (68%), Positives = 176/215 (81%)
 Frame = +3

Query: 3   LNKIMEEHRMESGREGGDLLTVLLGLKEDVNGDDEAKITDTNIKALLLNLFTAGTDTSAS 182
           L+K++ EH+  SG  G DLL+VL+ LK+D   D+  K++ T IKALLLNLFTAGTDT++S
Sbjct: 244 LDKLINEHKRGSG--GRDLLSVLVSLKDDEGEDEANKLSHTEIKALLLNLFTAGTDTTSS 301

Query: 183 TVEWAMAELIRHPDILVAVQAEIDAVTGRSRLVSEPDLAKLPLLRAIIKETFRLHPSTPL 362
           TVEWA+AELIRHP +L   Q E+D V GR RLVSE DL  LP L+AIIKETFRLHPSTPL
Sbjct: 302 TVEWALAELIRHPTLLHKAQQELDNVVGRQRLVSETDLPNLPFLQAIIKETFRLHPSTPL 361

Query: 363 SIPRTAAEPCEIGGFHVPSGATLLVNVWAIARDPEVWSDPLEFKPSRFLPGGSHADVDLK 542
           S+PR ++EPCEI G+++P  ATLLVNVWAIARDP VWSDPLEFKP RF+PGG  A+VD+K
Sbjct: 362 SLPRISSEPCEINGYYIPKNATLLVNVWAIARDPAVWSDPLEFKPERFMPGGEKANVDVK 421

Query: 543 GAHFELIPFGAGRRICAGLSLGLRMVTLMVASLVH 647
           G  FE+IPFGAGRRICAG+SLGLRMV  M A+LVH
Sbjct: 422 GNDFEVIPFGAGRRICAGMSLGLRMVQFMTATLVH 456


>gb|ADZ54783.1| flavonoid 3'-monooxygenase [Prunus avium]
          Length = 510

 Score =  301 bits (771), Expect = 7e-80
 Identities = 148/215 (68%), Positives = 175/215 (81%)
 Frame = +3

Query: 3   LNKIMEEHRMESGREGGDLLTVLLGLKEDVNGDDEAKITDTNIKALLLNLFTAGTDTSAS 182
           L  I+E+H+  SG +  D+LT LL LKED +G+  A++TDT IKALLLN+FTAGTDTS+S
Sbjct: 248 LTAIVEDHKKSSGGKHVDMLTTLLSLKEDADGEG-AQLTDTEIKALLLNMFTAGTDTSSS 306

Query: 183 TVEWAMAELIRHPDILVAVQAEIDAVTGRSRLVSEPDLAKLPLLRAIIKETFRLHPSTPL 362
           TVEWA+AEL+RHP IL  VQ E+D V GR RLV+E DL  L  L+A+IKETFRLHPSTPL
Sbjct: 307 TVEWAIAELLRHPKILAQVQQELDQVAGRDRLVTELDLPNLTYLQAVIKETFRLHPSTPL 366

Query: 363 SIPRTAAEPCEIGGFHVPSGATLLVNVWAIARDPEVWSDPLEFKPSRFLPGGSHADVDLK 542
           S+PR A+E CEI GFH+P GATLLVNVWAI+RDPE W DPLEF+P RFLPGG    VD++
Sbjct: 367 SLPRMASENCEINGFHIPKGATLLVNVWAISRDPEQWKDPLEFRPERFLPGGEKPHVDVR 426

Query: 543 GAHFELIPFGAGRRICAGLSLGLRMVTLMVASLVH 647
           G  FE+IPFGAGRRICAG+SLGLRMV LM A+LVH
Sbjct: 427 GNDFEVIPFGAGRRICAGMSLGLRMVHLMAATLVH 461


>gb|ADE80941.1| flavonoid 3'-hydroxylase [Epimedium sagittatum]
          Length = 514

 Score =  300 bits (769), Expect = 1e-79
 Identities = 146/218 (66%), Positives = 180/218 (82%), Gaps = 3/218 (1%)
 Frame = +3

Query: 3   LNKIMEEHRMESGREGG---DLLTVLLGLKEDVNGDDEAKITDTNIKALLLNLFTAGTDT 173
           LNKI+EEH+  +G +G    DLL+VL+GLK+D  G+   K+TDTNIKALLL+LFTAGTDT
Sbjct: 249 LNKILEEHKESNGEDGHVHTDLLSVLIGLKDDAEGEG-GKLTDTNIKALLLDLFTAGTDT 307

Query: 174 SASTVEWAMAELIRHPDILVAVQAEIDAVTGRSRLVSEPDLAKLPLLRAIIKETFRLHPS 353
           S+STVEWA+AELIR+P +L   Q E++ V GR RLVSE DL +L   +AIIKETFRLHPS
Sbjct: 308 SSSTVEWAIAELIRNPKLLAQAQEELNQVVGRDRLVSESDLGQLTFFQAIIKETFRLHPS 367

Query: 354 TPLSIPRTAAEPCEIGGFHVPSGATLLVNVWAIARDPEVWSDPLEFKPSRFLPGGSHADV 533
           TPLS+PR A+E CEI G+H+P  +TLLVNVWAIARDP+VWS+PLEFKP RFLPGG +A +
Sbjct: 368 TPLSLPRMASESCEIDGYHIPKNSTLLVNVWAIARDPDVWSEPLEFKPDRFLPGGKNAHM 427

Query: 534 DLKGAHFELIPFGAGRRICAGLSLGLRMVTLMVASLVH 647
           D+KG  FE+IPFGAGRRICAG+S+G+RMV  + A+LVH
Sbjct: 428 DVKGTDFEVIPFGAGRRICAGMSMGMRMVQYVTATLVH 465