BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00007749
         (655 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   216   4e-54
ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [V...   211   1e-52
emb|CAN68429.1| hypothetical protein VITISV_012133 [Vitis vinifera]   211   1e-52
ref|XP_002283235.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis ...   208   6e-52
emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]                206   2e-51

>ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 499

 Score =  216 bits (549), Expect = 4e-54
 Identities = 110/212 (51%), Positives = 146/212 (68%)
 Frame = -3

Query: 653 QALAHLSDLHGPILLLRFGSRRVLLVSSYSAADECFTVNDITFANRPRLLAGKYLGYDFT 474
           + L+ +SD HGPIL LRFGSR VLLVSS SA++ECFT ND+ FANRPRL+AGK+LGY++T
Sbjct: 51  RTLSKISDRHGPILFLRFGSRPVLLVSSPSASEECFTKNDVVFANRPRLIAGKHLGYNYT 110

Query: 473 SLGWAPYGPHWRNLRRMYTVELLSTNRLLSSSDVRSDEACSLVKALLREYSGPSFHRTEL 294
           S+ WAP+G HWRNLRR+ + E+LS+NRL + S +RSDE  SLV+ L +  S       E+
Sbjct: 111 SMSWAPHGDHWRNLRRISSFEILSSNRLQTLSGIRSDEVRSLVRWLFKNQS----QMVEM 166

Query: 293 KPKFFGLAYNVIMRMMANKRYYGDADESSSEAGTEFRDMVKETALALSASNAADFIPLAR 114
           K  FF +  NV+M+M+  KRYYG+ +    E    FR+MV ET     A+N  DF+P+  
Sbjct: 167 KSAFFEMTLNVMMKMIGGKRYYGE-NIGEVEEARMFREMVSETFQLAGATNMVDFLPILG 225

Query: 113 WFRVGGYETKLKSLRKRRDKFFQGLIQELREK 18
           W  + G E +L  L+KRR+ F Q LI+E R K
Sbjct: 226 WLGLKGTERRLIKLQKRRESFIQNLIEEHRRK 257


>ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 503

 Score =  211 bits (536), Expect = 1e-52
 Identities = 107/213 (50%), Positives = 146/213 (68%)
 Frame = -3

Query: 653 QALAHLSDLHGPILLLRFGSRRVLLVSSYSAADECFTVNDITFANRPRLLAGKYLGYDFT 474
           +  A +S+ +GPIL +RFGSR V++VSS SAA+ECFT NDI FANRPRLLAGK+LGY++T
Sbjct: 51  RTFAKISNQYGPILFIRFGSRPVIIVSSPSAAEECFTKNDIVFANRPRLLAGKHLGYNYT 110

Query: 473 SLGWAPYGPHWRNLRRMYTVELLSTNRLLSSSDVRSDEACSLVKALLREYSGPSFHRTEL 294
           +L WAPYG HWRNLRR+ ++E+LS+NRL    D+R DE  +L+  L R  S   F   ++
Sbjct: 111 TLTWAPYGQHWRNLRRIASLEILSSNRLQMFYDIRIDEVRALLCQLFRASSEGQFSAVDM 170

Query: 293 KPKFFGLAYNVIMRMMANKRYYGDADESSSEAGTEFRDMVKETALALSASNAADFIPLAR 114
           K  FF L  N +MRM++ KRYYGD + +  E   +FR++V ET     A+N  DF+P ++
Sbjct: 171 KSMFFELTLNNMMRMISGKRYYGD-NVTELEETRKFREIVAETFELSGATNIVDFVPFSK 229

Query: 113 WFRVGGYETKLKSLRKRRDKFFQGLIQELREKK 15
           W  + G E KL  L+ +RD F Q LI+E R  +
Sbjct: 230 WIGLNGIEKKLVILQGKRDGFMQNLIEEHRRMR 262


>emb|CAN68429.1| hypothetical protein VITISV_012133 [Vitis vinifera]
          Length = 1001

 Score =  211 bits (536), Expect = 1e-52
 Identities = 107/213 (50%), Positives = 146/213 (68%)
 Frame = -3

Query: 653  QALAHLSDLHGPILLLRFGSRRVLLVSSYSAADECFTVNDITFANRPRLLAGKYLGYDFT 474
            +  A +S+ +GPIL +RFGSR V++VSS SAA+ECFT NDI FANRPRLLAGK+LGY++T
Sbjct: 549  RTFAKISNQYGPILFIRFGSRPVIIVSSPSAAEECFTKNDIVFANRPRLLAGKHLGYNYT 608

Query: 473  SLGWAPYGPHWRNLRRMYTVELLSTNRLLSSSDVRSDEACSLVKALLREYSGPSFHRTEL 294
            +L WAPYG HWRNLRR+ ++E+LS+NRL    D+R DE  +L+  L R  S   F   ++
Sbjct: 609  TLTWAPYGQHWRNLRRIASLEILSSNRLQMFYDIRIDEVRALLCQLFRASSEGQFSAVDM 668

Query: 293  KPKFFGLAYNVIMRMMANKRYYGDADESSSEAGTEFRDMVKETALALSASNAADFIPLAR 114
            K  FF L  N +MRM++ KRYYGD + +  E   +FR++V ET     A+N  DF+P ++
Sbjct: 669  KSMFFELTLNNMMRMISGKRYYGD-NVTELEETRKFREIVAETFELSGATNIVDFVPFSK 727

Query: 113  WFRVGGYETKLKSLRKRRDKFFQGLIQELREKK 15
            W  + G E KL  L+ +RD F Q LI+E R  +
Sbjct: 728  WIGLNGIEKKLVILQGKRDGFMQNLIEEHRRMR 760


>ref|XP_002283235.1| PREDICTED: isoflavone 2'-hydroxylase [Vitis vinifera]
          Length = 498

 Score =  208 bits (530), Expect = 6e-52
 Identities = 110/213 (51%), Positives = 146/213 (68%)
 Frame = -3

Query: 647 LAHLSDLHGPILLLRFGSRRVLLVSSYSAADECFTVNDITFANRPRLLAGKYLGYDFTSL 468
           L+ +SD HGPIL LRFG R VL+VSS+SAA+ECFT NDI FANRPRL+AGK+LGY++T++
Sbjct: 53  LSKISDRHGPILFLRFGYRPVLIVSSHSAAEECFTKNDIIFANRPRLIAGKHLGYNYTAI 112

Query: 467 GWAPYGPHWRNLRRMYTVELLSTNRLLSSSDVRSDEACSLVKALLREYSGPSFHRTELKP 288
           G APYG HWRNLRR+ + E+LS+NRL   S +RSDE  +L+  L++  +       E+K 
Sbjct: 113 GTAPYGDHWRNLRRISSFEILSSNRLQMLSGIRSDEVRTLICRLVKNQN----QVVEMKS 168

Query: 287 KFFGLAYNVIMRMMANKRYYGDADESSSEAGTEFRDMVKETALALSASNAADFIPLARWF 108
            FF L  NVIMRM+  KRYYG+ +    E   +FR+MV + A  L+ +N  DF+P+  W 
Sbjct: 169 AFFELTLNVIMRMIGGKRYYGE-NVGEVEEARKFREMVSK-AFRLAGTNMVDFLPILGWL 226

Query: 107 RVGGYETKLKSLRKRRDKFFQGLIQELREKKMK 9
            + G E +L  L+K RD F Q LI+E R K  K
Sbjct: 227 GLKGTEKRLMELQKMRDSFIQNLIEEHRIKGSK 259


>emb|CAA04117.1| cytochrome P450 [Helianthus tuberosus]
          Length = 520

 Score =  206 bits (525), Expect = 2e-51
 Identities = 106/213 (49%), Positives = 144/213 (67%)
 Frame = -3

Query: 653 QALAHLSDLHGPILLLRFGSRRVLLVSSYSAADECFTVNDITFANRPRLLAGKYLGYDFT 474
           + LA LS  HGPIL L+ G RRVL+VSS SAA+ECFT NDI FANRP++L GK +G ++T
Sbjct: 67  RTLAKLSAKHGPILRLQLGFRRVLIVSSPSAAEECFTKNDIVFANRPKMLFGKIIGVNYT 126

Query: 473 SLGWAPYGPHWRNLRRMYTVELLSTNRLLSSSDVRSDEACSLVKALLREYSGPSFHRTEL 294
           SL W+PYG +WRNLRR+ ++E+LS +RL    D+R +E   L++ LL   +  S   T +
Sbjct: 127 SLAWSPYGDNWRNLRRIASIEILSIHRLNEFHDIRVEETRLLIQKLLSACNSGSSQVT-M 185

Query: 293 KPKFFGLAYNVIMRMMANKRYYGDADESSSEAGTEFRDMVKETALALSASNAADFIPLAR 114
           K  F+ L  NV+MRM++ KRY+G  +    E G  FRDM+ ET +   ASN  D++P+  
Sbjct: 186 KFSFYELTLNVMMRMISGKRYFGGDNPELEEEGKRFRDMLDETFVLAGASNVGDYLPVLS 245

Query: 113 WFRVGGYETKLKSLRKRRDKFFQGLIQELREKK 15
           W  V G E KL  L+++RD FFQGLI +LR+ K
Sbjct: 246 WLGVKGLEKKLIKLQEKRDVFFQGLIDQLRKSK 278