BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00006464
         (1304 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002524955.1| ubiquitin-protein ligase, putative [Ricinus ...   281   1e-99
ref|XP_002332232.1| predicted protein [Populus trichocarpa] gi|2...   281   8e-98
ref|XP_002306495.1| predicted protein [Populus trichocarpa] gi|2...   283   5e-97
dbj|BAJ92958.1| predicted protein [Hordeum vulgare subsp. vulgare]    276   1e-96
gb|EEC82795.1| hypothetical protein OsI_27558 [Oryza sativa Indi...   275   4e-95

>ref|XP_002524955.1| ubiquitin-protein ligase, putative [Ricinus communis]
            gi|223535790|gb|EEF37452.1| ubiquitin-protein ligase,
            putative [Ricinus communis]
          Length = 799

 Score =  281 bits (720), Expect(2) = 1e-99
 Identities = 142/192 (73%), Positives = 167/192 (86%)
 Frame = +2

Query: 530  IDPLVHVLETGNAEAKENSAAAIFNLSALKELMLRIGRSGAIKPLVDLLENGSPQGKKDA 709
            I PL+HVLETG+ EAKENSAA +F+LS +++  +RIGRSGA+ PLVDLL NG+P+GKKDA
Sbjct: 600  IGPLIHVLETGSPEAKENSAATLFSLSVIEDNKVRIGRSGAVGPLVDLLGNGTPRGKKDA 659

Query: 710  IRALFNLSVITENKPRIVEAGAIRPLIALMDPALGMVDKAVAILANLATVSEAQIAIGRE 889
              ALFNLS+  ENK RIV+AGA++ L+ LMDPA GMVDKAVA+LANLAT+ E + AIG+E
Sbjct: 660  ATALFNLSIFHENKARIVQAGAVKHLVELMDPAAGMVDKAVAVLANLATIPEGRTAIGQE 719

Query: 890  GGIPVLVEVVELGSARGKENAAAVLLRLCTSSKRFCRQVLKEGAVPPLAALSQSGTPRAK 1069
            GGIPVLVEVVELGSARGKENAAA LL+LCT+S RFC  VL+EGAVPPL ALSQSGTPRAK
Sbjct: 720  GGIPVLVEVVELGSARGKENAAAALLQLCTNSNRFCNTVLQEGAVPPLVALSQSGTPRAK 779

Query: 1070 EKARSLLCFFRN 1105
            EKA++LL FFRN
Sbjct: 780  EKAQALLSFFRN 791



 Score =  109 bits (272), Expect(2) = 1e-99
 Identities = 57/108 (52%), Positives = 79/108 (73%), Gaps = 1/108 (0%)
 Frame = +3

Query: 102 PDFLRRLAEAQSGSQKHRRRFPEKNGQNIITLPSMDLKPENSGIETQVHKLIEDLKCESV 281
           P+F  R  E +S SQ   RR  ++    I++ P+++ + + SG+ETQV  L+EDLK +S+
Sbjct: 470 PEFAPRFIETRSRSQTIWRRPSDRLIPRIVSSPAIETRADLSGVETQVRNLVEDLKSDSI 529

Query: 282 DVQRVATSELRMLTKH-MENRIVIANCGAISLLISLLYSTDLGIQENA 422
           DVQR AT+ELR+L KH M+NRIVIANCGAI++L++LL S D  IQENA
Sbjct: 530 DVQRAATAELRLLAKHNMDNRIVIANCGAINILVNLLRSADAKIQENA 577



 Score = 71.6 bits (174), Expect = 4e-10
 Identities = 46/137 (33%), Positives = 76/137 (55%), Gaps = 1/137 (0%)
 Frame = +2

Query: 635  IGRSGAIKPLVDLLENGSPQGKKDAIRALFNLSVITENKPRIVEAGAIRPLIALMDPALG 814
            I   GAI  LV+LL +   + +++A+ AL NLS+   NK  I  A AI PLI +++    
Sbjct: 553  IANCGAINILVNLLRSADAKIQENAVTALLNLSINDNNKTAIANADAIGPLIHVLETGSP 612

Query: 815  MV-DKAVAILANLATVSEAQIAIGREGGIPVLVEVVELGSARGKENAAAVLLRLCTSSKR 991
               + + A L +L+ + + ++ IGR G +  LV+++  G+ RGK++AA  L  L    + 
Sbjct: 613  EAKENSAATLFSLSVIEDNKVRIGRSGAVGPLVDLLGNGTPRGKKDAATALFNLSIFHEN 672

Query: 992  FCRQVLKEGAVPPLAAL 1042
              R +++ GAV  L  L
Sbjct: 673  KAR-IVQAGAVKHLVEL 688


>ref|XP_002332232.1| predicted protein [Populus trichocarpa] gi|222831845|gb|EEE70322.1|
            predicted protein [Populus trichocarpa]
          Length = 698

 Score =  281 bits (720), Expect(2) = 8e-98
 Identities = 142/192 (73%), Positives = 167/192 (86%)
 Frame = +2

Query: 530  IDPLVHVLETGNAEAKENSAAAIFNLSALKELMLRIGRSGAIKPLVDLLENGSPQGKKDA 709
            I+PL+HVLETG+ EAKENSAA +F+LS +++  +RIGRSGA+ PLVDLL NG+P+GKKDA
Sbjct: 499  IEPLIHVLETGSPEAKENSAATLFSLSVIEDNKVRIGRSGAVGPLVDLLGNGTPRGKKDA 558

Query: 710  IRALFNLSVITENKPRIVEAGAIRPLIALMDPALGMVDKAVAILANLATVSEAQIAIGRE 889
              ALFNLS+  ENK RIVEAGA++ L+ LMDPA GMVDKAVA+LANLAT+ E + AIG+E
Sbjct: 559  ATALFNLSIFHENKARIVEAGAVKHLVDLMDPAAGMVDKAVAVLANLATIPEGRNAIGQE 618

Query: 890  GGIPVLVEVVELGSARGKENAAAVLLRLCTSSKRFCRQVLKEGAVPPLAALSQSGTPRAK 1069
            GGIPVLVEVVELGS RGKENAAA LL+LCT+S RFC  VL+EGAVPPL ALSQSGTPRAK
Sbjct: 619  GGIPVLVEVVELGSVRGKENAAAALLQLCTNSSRFCHMVLQEGAVPPLVALSQSGTPRAK 678

Query: 1070 EKARSLLCFFRN 1105
            EKA+SLL +FRN
Sbjct: 679  EKAQSLLSYFRN 690



 Score =  103 bits (257), Expect(2) = 8e-98
 Identities = 60/130 (46%), Positives = 88/130 (67%), Gaps = 1/130 (0%)
 Frame = +3

Query: 36  PEMSLFRGA*EISMASSVSQARPDFLRRLAEAQSGSQKHRRRFPEKNGQNIITLPSMDLK 215
           PE +   G+   SM SS    +P+F   L + +S SQ   RR  ++    I++ P+++ +
Sbjct: 351 PESNRSAGSPGRSMISS----KPEFPSFLVDTRSRSQTAWRRPSDRLVPRIVSSPAIETR 406

Query: 216 PENSGIETQVHKLIEDLKCESVDVQRVATSELRMLTKH-MENRIVIANCGAISLLISLLY 392
            + +GIE +V KL+EDL+  S+D+QR AT++LR+L KH M+NRIVIANCG+I LL++LL 
Sbjct: 407 ADLAGIEMEVRKLVEDLRSTSIDIQRDATAKLRLLAKHNMDNRIVIANCGSIGLLVNLLC 466

Query: 393 STDLGIQENA 422
           STD  IQENA
Sbjct: 467 STDKKIQENA 476



 Score = 67.8 bits (164), Expect = 6e-09
 Identities = 45/137 (32%), Positives = 76/137 (55%), Gaps = 1/137 (0%)
 Frame = +2

Query: 635  IGRSGAIKPLVDLLENGSPQGKKDAIRALFNLSVITENKPRIVEAGAIRPLIALMDPALG 814
            I   G+I  LV+LL +   + +++A+ AL NLS+   NK  I  A AI PLI +++    
Sbjct: 452  IANCGSIGLLVNLLCSTDKKIQENAVTALLNLSINDNNKTAIANADAIEPLIHVLETGSP 511

Query: 815  MV-DKAVAILANLATVSEAQIAIGREGGIPVLVEVVELGSARGKENAAAVLLRLCTSSKR 991
               + + A L +L+ + + ++ IGR G +  LV+++  G+ RGK++AA  L  L    + 
Sbjct: 512  EAKENSAATLFSLSVIEDNKVRIGRSGAVGPLVDLLGNGTPRGKKDAATALFNLSIFHEN 571

Query: 992  FCRQVLKEGAVPPLAAL 1042
              R +++ GAV  L  L
Sbjct: 572  KAR-IVEAGAVKHLVDL 587


>ref|XP_002306495.1| predicted protein [Populus trichocarpa] gi|222855944|gb|EEE93491.1|
            predicted protein [Populus trichocarpa]
          Length = 822

 Score =  283 bits (723), Expect(2) = 5e-97
 Identities = 143/192 (74%), Positives = 168/192 (87%)
 Frame = +2

Query: 530  IDPLVHVLETGNAEAKENSAAAIFNLSALKELMLRIGRSGAIKPLVDLLENGSPQGKKDA 709
            I+PL+HVLETG+ EAKENSAA +F+LS +++  +RIGRSGAI PLVDLL NG+P+GKKDA
Sbjct: 622  IEPLIHVLETGSPEAKENSAATLFSLSVIEDNKVRIGRSGAIVPLVDLLGNGTPRGKKDA 681

Query: 710  IRALFNLSVITENKPRIVEAGAIRPLIALMDPALGMVDKAVAILANLATVSEAQIAIGRE 889
              ALFNLS+  ENK RIV+AGA++ L+ LMDPA GMVDKAVA+LANLAT+ E + AIG+E
Sbjct: 682  ATALFNLSIFHENKDRIVQAGAVKHLVELMDPAAGMVDKAVAVLANLATIPEGRNAIGQE 741

Query: 890  GGIPVLVEVVELGSARGKENAAAVLLRLCTSSKRFCRQVLKEGAVPPLAALSQSGTPRAK 1069
            GGIPVLVEVVELGSARGKENAAA LL+LCT+S RFC  VL+EGAVPPL ALSQSGTPRAK
Sbjct: 742  GGIPVLVEVVELGSARGKENAAAALLQLCTNSSRFCHMVLQEGAVPPLVALSQSGTPRAK 801

Query: 1070 EKARSLLCFFRN 1105
            EKA++LL FFRN
Sbjct: 802  EKAQALLSFFRN 813



 Score = 99.8 bits (247), Expect(2) = 5e-97
 Identities = 57/120 (47%), Positives = 80/120 (66%), Gaps = 1/120 (0%)
 Frame = +3

Query: 66  EISMASSVSQARPDFLRRLAEAQSGSQKHRRRFPEKNGQNIITLPSMDLKPENSGIETQV 245
           + S A       P+F  RL + +S SQ   RR  ++    I++  +++ + + +GIET+V
Sbjct: 480 QASSALHTPHREPEFPSRLVDTRSRSQTIWRRPSDRLVPRIVSSSAIETRADLAGIETEV 539

Query: 246 HKLIEDLKCESVDVQRVATSELRMLTKH-MENRIVIANCGAISLLISLLYSTDLGIQENA 422
             L+EDLK   VD QR AT++LR+L KH M+NRIVIAN GAISLL++LL STD+ IQENA
Sbjct: 540 RNLVEDLKSTLVDTQRDATAKLRLLAKHNMDNRIVIANFGAISLLVNLLRSTDIKIQENA 599



 Score = 68.6 bits (166), Expect = 3e-09
 Identities = 47/137 (34%), Positives = 76/137 (55%), Gaps = 1/137 (0%)
 Frame = +2

Query: 635  IGRSGAIKPLVDLLENGSPQGKKDAIRALFNLSVITENKPRIVEAGAIRPLIALMDPALG 814
            I   GAI  LV+LL +   + +++A+ AL NLS+   NK  I  A AI PLI +++    
Sbjct: 575  IANFGAISLLVNLLRSTDIKIQENAVTALLNLSINDNNKTAIGNADAIEPLIHVLETGSP 634

Query: 815  MV-DKAVAILANLATVSEAQIAIGREGGIPVLVEVVELGSARGKENAAAVLLRLCTSSKR 991
               + + A L +L+ + + ++ IGR G I  LV+++  G+ RGK++AA  L  L    + 
Sbjct: 635  EAKENSAATLFSLSVIEDNKVRIGRSGAIVPLVDLLGNGTPRGKKDAATALFNLSIFHEN 694

Query: 992  FCRQVLKEGAVPPLAAL 1042
              R +++ GAV  L  L
Sbjct: 695  KDR-IVQAGAVKHLVEL 710


>dbj|BAJ92958.1| predicted protein [Hordeum vulgare subsp. vulgare]
          Length = 831

 Score =  276 bits (705), Expect(2) = 1e-96
 Identities = 139/192 (72%), Positives = 166/192 (86%)
 Frame = +2

Query: 530  IDPLVHVLETGNAEAKENSAAAIFNLSALKELMLRIGRSGAIKPLVDLLENGSPQGKKDA 709
            +DPL+HVLETGN EAKENSAA +F+LS ++E  +RIGRSGA+KPLVDLL NG+P+GKKDA
Sbjct: 631  VDPLIHVLETGNPEAKENSAATLFSLSVIEENKVRIGRSGAVKPLVDLLGNGTPRGKKDA 690

Query: 710  IRALFNLSVITENKPRIVEAGAIRPLIALMDPALGMVDKAVAILANLATVSEAQIAIGRE 889
              ALFNLS++ ENK RIV+A A+R L+ LMDPA GMVDKAVA+LANLAT+ E + AIG+ 
Sbjct: 691  ATALFNLSILHENKGRIVQADAVRYLVELMDPAAGMVDKAVAVLANLATIPEGRTAIGQA 750

Query: 890  GGIPVLVEVVELGSARGKENAAAVLLRLCTSSKRFCRQVLKEGAVPPLAALSQSGTPRAK 1069
             GIP LVEVVELGSARGKENAAA LL+LCT+S RFC  VL+EGAVPPL ALSQSGTPRA+
Sbjct: 751  RGIPALVEVVELGSARGKENAAAALLQLCTNSNRFCSIVLQEGAVPPLVALSQSGTPRAR 810

Query: 1070 EKARSLLCFFRN 1105
            EKA++LL +FR+
Sbjct: 811  EKAQALLSYFRS 822



 Score =  105 bits (261), Expect(2) = 1e-96
 Identities = 59/115 (51%), Positives = 78/115 (67%), Gaps = 1/115 (0%)
 Frame = +3

Query: 81  SSVSQARPDFLRRLAEAQSGSQKHRRRFPEKNGQNIITLPSMDLKPENSGIETQVHKLIE 260
           SS  Q     L RL + +   Q  RR+  ++    I++  SMD + + S IE QV KLI+
Sbjct: 494 SSAPQRENVMLPRLGDVRMRGQFVRRQPSDRGFPRILSSSSMDARGDLSAIENQVRKLID 553

Query: 261 DLKCESVDVQRVATSELRMLTKH-MENRIVIANCGAISLLISLLYSTDLGIQENA 422
           DLK +S++ QR ATSE+R+L KH MENRIVIANCGAI+LL+ LL+S+D  IQENA
Sbjct: 554 DLKSDSIEAQRSATSEIRLLAKHNMENRIVIANCGAINLLVGLLHSSDAKIQENA 608



 Score = 70.5 bits (171), Expect = 9e-10
 Identities = 47/147 (31%), Positives = 82/147 (55%), Gaps = 2/147 (1%)
 Frame = +2

Query: 539 LVHVLETGNAEAKENSAAAIFNLSALK-ELMLRIGRSGAIKPLVDLLENGSPQGKKDAIR 715
           L+  L++ + EA+ ++ + I  L+    E  + I   GAI  LV LL +   + +++A+ 
Sbjct: 551 LIDDLKSDSIEAQRSATSEIRLLAKHNMENRIVIANCGAINLLVGLLHSSDAKIQENAVT 610

Query: 716 ALFNLSVITENKPRIVEAGAIRPLIALMDPALGMV-DKAVAILANLATVSEAQIAIGREG 892
           AL NLS+   NK  I  A A+ PLI +++       + + A L +L+ + E ++ IGR G
Sbjct: 611 ALLNLSINDNNKIAIASADAVDPLIHVLETGNPEAKENSAATLFSLSVIEENKVRIGRSG 670

Query: 893 GIPVLVEVVELGSARGKENAAAVLLRL 973
            +  LV+++  G+ RGK++AA  L  L
Sbjct: 671 AVKPLVDLLGNGTPRGKKDAATALFNL 697


>gb|EEC82795.1| hypothetical protein OsI_27558 [Oryza sativa Indica Group]
          Length = 839

 Score =  275 bits (704), Expect(2) = 4e-95
 Identities = 139/192 (72%), Positives = 166/192 (86%)
 Frame = +2

Query: 530  IDPLVHVLETGNAEAKENSAAAIFNLSALKELMLRIGRSGAIKPLVDLLENGSPQGKKDA 709
            +DPL+HVLETGN EAKENSAA +F+LS ++E  +RIGRSGAIKPLVDLL NG+P+GKKDA
Sbjct: 639  VDPLIHVLETGNPEAKENSAATLFSLSVIEENKVRIGRSGAIKPLVDLLGNGTPRGKKDA 698

Query: 710  IRALFNLSVITENKPRIVEAGAIRPLIALMDPALGMVDKAVAILANLATVSEAQIAIGRE 889
              ALFNLS++ ENK RIV+A A++ L+ LMDPA GMVDKAVA+LANLAT+ E + AIG+ 
Sbjct: 699  ATALFNLSILHENKARIVQADAVKYLVELMDPAAGMVDKAVAVLANLATIPEGRTAIGQA 758

Query: 890  GGIPVLVEVVELGSARGKENAAAVLLRLCTSSKRFCRQVLKEGAVPPLAALSQSGTPRAK 1069
             GIP LVEVVELGSARGKENAAA LL+LCT+S RFC  VL+EGAVPPL ALSQSGTPRA+
Sbjct: 759  RGIPALVEVVELGSARGKENAAAALLQLCTNSSRFCSIVLQEGAVPPLVALSQSGTPRAR 818

Query: 1070 EKARSLLCFFRN 1105
            EKA++LL +FR+
Sbjct: 819  EKAQALLSYFRS 830



 Score =  100 bits (250), Expect(2) = 4e-95
 Identities = 60/116 (51%), Positives = 78/116 (67%), Gaps = 1/116 (0%)
 Frame = +3

Query: 78  ASSVSQARPDFLRRLAEAQSGSQKHRRRFPEKNGQNIITLPSMDLKPENSGIETQVHKLI 257
           ASS  Q       RLA+ +S  Q  RR   E+    II+  SMD + + S IE QV KL+
Sbjct: 502 ASSAPQREHLIPSRLADVRSRGQFVRRP-SERGFPRIISSSSMDTRSDLSAIENQVRKLV 560

Query: 258 EDLKCESVDVQRVATSELRMLTKH-MENRIVIANCGAISLLISLLYSTDLGIQENA 422
           +DL+ +SVDVQR ATS++R+L KH MENRI+IANCGAI+LL+ LL+S D   QE+A
Sbjct: 561 DDLRSDSVDVQRSATSDIRLLAKHNMENRIIIANCGAINLLVGLLHSPDSKTQEHA 616



 Score = 67.4 bits (163), Expect = 8e-09
 Identities = 41/114 (35%), Positives = 64/114 (56%), Gaps = 1/114 (0%)
 Frame = +2

Query: 635 IGRSGAIKPLVDLLENGSPQGKKDAIRALFNLSVITENKPRIVEAGAIRPLIALMDPALG 814
           I   GAI  LV LL +   + ++ A+ AL NLS+   NK  I  A A+ PLI +++    
Sbjct: 592 IANCGAINLLVGLLHSPDSKTQEHAVTALLNLSINDNNKIAIANADAVDPLIHVLETGNP 651

Query: 815 MV-DKAVAILANLATVSEAQIAIGREGGIPVLVEVVELGSARGKENAAAVLLRL 973
              + + A L +L+ + E ++ IGR G I  LV+++  G+ RGK++AA  L  L
Sbjct: 652 EAKENSAATLFSLSVIEENKVRIGRSGAIKPLVDLLGNGTPRGKKDAATALFNL 705