BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00006413
         (1279 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI19072.3| unnamed protein product [Vitis vinifera]              428   e-117
ref|XP_002285834.1| PREDICTED: biotin--protein ligase [Vitis vin...   428   e-117
ref|XP_002307419.1| predicted protein [Populus trichocarpa] gi|2...   427   e-117
ref|XP_003601098.1| Holocarboxylase synthetase hcs2 [Medicago tr...   423   e-116
ref|XP_002893926.1| holocarboxylase synthetase 2 [Arabidopsis ly...   423   e-116

>emb|CBI19072.3| unnamed protein product [Vitis vinifera]
          Length = 416

 Score =  428 bits (1101), Expect = e-117
 Identities = 202/316 (63%), Positives = 248/316 (78%)
 Frame = -3

Query: 1142 CSKSPREFDLALTSKNAGFKLEGVEEEIRVFIQSDLDPGIQQTFQTDVYMGALSTNLFGR 963
            C KS  E + A + K           E+ + +  +++   ++ FQ D++M +LSTN FGR
Sbjct: 101  CGKSSAEIEFAKSLKAKNTLKLPDNGEVSILLHPEIEKPEEEPFQMDLFMSSLSTNTFGR 160

Query: 962  LLIWSPRLPSTHDVVSQNFCEIPLGTVCVTDLQFKGRGRSKNVWESPPGCVMFSFTLEME 783
             LIWSP+LPSTHDVVSQNFCE+P+GTVCV D+Q KGRGRSKN WESP GC++FSFTL+ME
Sbjct: 161  FLIWSPQLPSTHDVVSQNFCELPIGTVCVADVQLKGRGRSKNKWESPRGCLLFSFTLQME 220

Query: 782  DGRKLPLLQYVVSLAITEAIKDVCRMQGLPELDVRIKWPNDLYLNGLKVGGILCTSTYRS 603
            DGR +PLLQYVVSLAITEAIK +C  +GLP LDV+IKWPNDLYLNGLKVGGILCTSTY+S
Sbjct: 221  DGRTVPLLQYVVSLAITEAIKSICDSKGLPHLDVKIKWPNDLYLNGLKVGGILCTSTYKS 280

Query: 602  KKFHVCAGVGLNLDNEKPTTCLNAALKEMIPDSGQIKREDLVAAFFNKFENLFEVFLGQG 423
            +KF+V AG+GLN+DNEKPTTCLNA L+E+   + Q +RED+VAAFFNKFE  F +F+ QG
Sbjct: 281  RKFNVSAGIGLNVDNEKPTTCLNAVLRELSSVAYQFRREDIVAAFFNKFETFFSLFINQG 340

Query: 422  FQALEELYYQTWLHSGQKVVIEEKNNGHVENIAVIIQGLTPSGYLLATDEENKEFELHPD 243
            FQ LEELY +TWLHSGQ+V+++E   G +    V IQGL  SGYLLA  ++ +  ELHPD
Sbjct: 341  FQTLEELYCKTWLHSGQRVIVQENIEGQMVENVVTIQGLASSGYLLAIGDDGQMCELHPD 400

Query: 242  GNSFDFFKGLVRRKLE 195
            GNSFDFFKGL+RRKLE
Sbjct: 401  GNSFDFFKGLIRRKLE 416


>ref|XP_002285834.1| PREDICTED: biotin--protein ligase [Vitis vinifera]
          Length = 327

 Score =  428 bits (1101), Expect = e-117
 Identities = 202/316 (63%), Positives = 248/316 (78%)
 Frame = -3

Query: 1142 CSKSPREFDLALTSKNAGFKLEGVEEEIRVFIQSDLDPGIQQTFQTDVYMGALSTNLFGR 963
            C KS  E + A + K           E+ + +  +++   ++ FQ D++M +LSTN FGR
Sbjct: 12   CGKSSAEIEFAKSLKAKNTLKLPDNGEVSILLHPEIEKPEEEPFQMDLFMSSLSTNTFGR 71

Query: 962  LLIWSPRLPSTHDVVSQNFCEIPLGTVCVTDLQFKGRGRSKNVWESPPGCVMFSFTLEME 783
             LIWSP+LPSTHDVVSQNFCE+P+GTVCV D+Q KGRGRSKN WESP GC++FSFTL+ME
Sbjct: 72   FLIWSPQLPSTHDVVSQNFCELPIGTVCVADVQLKGRGRSKNKWESPRGCLLFSFTLQME 131

Query: 782  DGRKLPLLQYVVSLAITEAIKDVCRMQGLPELDVRIKWPNDLYLNGLKVGGILCTSTYRS 603
            DGR +PLLQYVVSLAITEAIK +C  +GLP LDV+IKWPNDLYLNGLKVGGILCTSTY+S
Sbjct: 132  DGRTVPLLQYVVSLAITEAIKSICDSKGLPHLDVKIKWPNDLYLNGLKVGGILCTSTYKS 191

Query: 602  KKFHVCAGVGLNLDNEKPTTCLNAALKEMIPDSGQIKREDLVAAFFNKFENLFEVFLGQG 423
            +KF+V AG+GLN+DNEKPTTCLNA L+E+   + Q +RED+VAAFFNKFE  F +F+ QG
Sbjct: 192  RKFNVSAGIGLNVDNEKPTTCLNAVLRELSSVAYQFRREDIVAAFFNKFETFFSLFINQG 251

Query: 422  FQALEELYYQTWLHSGQKVVIEEKNNGHVENIAVIIQGLTPSGYLLATDEENKEFELHPD 243
            FQ LEELY +TWLHSGQ+V+++E   G +    V IQGL  SGYLLA  ++ +  ELHPD
Sbjct: 252  FQTLEELYCKTWLHSGQRVIVQENIEGQMVENVVTIQGLASSGYLLAIGDDGQMCELHPD 311

Query: 242  GNSFDFFKGLVRRKLE 195
            GNSFDFFKGL+RRKLE
Sbjct: 312  GNSFDFFKGLIRRKLE 327


>ref|XP_002307419.1| predicted protein [Populus trichocarpa] gi|222856868|gb|EEE94415.1|
            predicted protein [Populus trichocarpa]
          Length = 331

 Score =  427 bits (1098), Expect = e-117
 Identities = 207/319 (64%), Positives = 251/319 (78%), Gaps = 3/319 (0%)
 Frame = -3

Query: 1142 CSKSPREFDLALTSKNAGFKLEGVEEEIRVFIQSDLDPGIQQ---TFQTDVYMGALSTNL 972
            C KS  E ++A +  N          EI     S++    QQ   +F    +M +LSTNL
Sbjct: 13   CGKSSAENEIAKSLMNNNTLKLPDNVEISTLSHSEILDEPQQNEESFSLSRFMNSLSTNL 72

Query: 971  FGRLLIWSPRLPSTHDVVSQNFCEIPLGTVCVTDLQFKGRGRSKNVWESPPGCVMFSFTL 792
            FGRLLIWSP LPSTHD+VS NF E+P+GTVC+ D+Q+KGRGRSKNVWESP GC+MFSFT+
Sbjct: 73   FGRLLIWSPLLPSTHDLVSNNFGELPIGTVCIADVQYKGRGRSKNVWESPAGCLMFSFTI 132

Query: 791  EMEDGRKLPLLQYVVSLAITEAIKDVCRMQGLPELDVRIKWPNDLYLNGLKVGGILCTST 612
            +MEDG+ +PLLQYVVSLA+TEAIKD+C   GLP +DVRIKWPNDLYLNG+KVGGILCTST
Sbjct: 133  QMEDGQVVPLLQYVVSLAVTEAIKDLCDKNGLPHIDVRIKWPNDLYLNGVKVGGILCTST 192

Query: 611  YRSKKFHVCAGVGLNLDNEKPTTCLNAALKEMIPDSGQIKREDLVAAFFNKFENLFEVFL 432
            YRSKKF++ AG+GLN+DNEKPTTCLNA L+E+   +  ++RED+VAAF NKFE L+++F+
Sbjct: 193  YRSKKFNISAGIGLNVDNEKPTTCLNAVLRELAGAACALRREDIVAAFLNKFEKLYDLFI 252

Query: 431  GQGFQALEELYYQTWLHSGQKVVIEEKNNGHVENIAVIIQGLTPSGYLLATDEENKEFEL 252
              GFQ LEELYY+TWLHSGQ+V+I+EKN   V    V IQGLTPSGYLLA  E+N+  EL
Sbjct: 253  NGGFQTLEELYYKTWLHSGQRVIIQEKNENQVVENVVTIQGLTPSGYLLAIGEDNQMCEL 312

Query: 251  HPDGNSFDFFKGLVRRKLE 195
            HPDGNSFDFFKGLVRRK+E
Sbjct: 313  HPDGNSFDFFKGLVRRKIE 331


>ref|XP_003601098.1| Holocarboxylase synthetase hcs2 [Medicago truncatula]
            gi|355490146|gb|AES71349.1| Holocarboxylase synthetase
            hcs2 [Medicago truncatula]
          Length = 361

 Score =  423 bits (1088), Expect = e-116
 Identities = 208/319 (65%), Positives = 254/319 (79%), Gaps = 3/319 (0%)
 Frame = -3

Query: 1142 CSKSPREFDLALTSK-NAGFKLEGVEEEIRVFIQSDLDPGI--QQTFQTDVYMGALSTNL 972
            C KS  E + A+  K N   KL   ++E+ + + S+ +  +  +Q+FQ   +M +LSTN 
Sbjct: 44   CGKSLAENETAIAIKTNNTLKLPD-KKELSIILHSEFNKSVIQRQSFQFSSFMNSLSTNQ 102

Query: 971  FGRLLIWSPRLPSTHDVVSQNFCEIPLGTVCVTDLQFKGRGRSKNVWESPPGCVMFSFTL 792
            FGRLLIWSP L STHDVVS NFCE+P+GTVCV D+Q KGRGRSKN WESP GC+MFSFTL
Sbjct: 103  FGRLLIWSPELTSTHDVVSHNFCELPVGTVCVGDIQNKGRGRSKNAWESPLGCLMFSFTL 162

Query: 791  EMEDGRKLPLLQYVVSLAITEAIKDVCRMQGLPELDVRIKWPNDLYLNGLKVGGILCTST 612
            +MEDGR +PL+QYVVSLAITEAIKD+C   GLP +DV+IKWPNDLYLNG KVGGILCTST
Sbjct: 163  QMEDGRIVPLVQYVVSLAITEAIKDICDKNGLPCVDVKIKWPNDLYLNGFKVGGILCTST 222

Query: 611  YRSKKFHVCAGVGLNLDNEKPTTCLNAALKEMIPDSGQIKREDLVAAFFNKFENLFEVFL 432
            YRSKKF++ AG+GLN++NEKPTT LN  L+E+   + Q +RED++AAFFNKFE  F++FL
Sbjct: 223  YRSKKFNISAGIGLNVNNEKPTTSLNTVLRELSVGAYQFQREDVLAAFFNKFEKFFDLFL 282

Query: 431  GQGFQALEELYYQTWLHSGQKVVIEEKNNGHVENIAVIIQGLTPSGYLLATDEENKEFEL 252
             QGFQ LEELYY+TWLHSGQ+V+++EKN   V   AV IQGLT SGYLLA  ++N+  EL
Sbjct: 283  NQGFQTLEELYYKTWLHSGQRVIVQEKNEEKVVEHAVTIQGLTSSGYLLAVGDDNQMCEL 342

Query: 251  HPDGNSFDFFKGLVRRKLE 195
            HPDGNSFDFFKGLVRRKL+
Sbjct: 343  HPDGNSFDFFKGLVRRKLD 361


>ref|XP_002893926.1| holocarboxylase synthetase 2 [Arabidopsis lyrata subsp. lyrata]
            gi|297339768|gb|EFH70185.1| holocarboxylase synthetase 2
            [Arabidopsis lyrata subsp. lyrata]
          Length = 328

 Score =  423 bits (1088), Expect = e-116
 Identities = 203/317 (64%), Positives = 250/317 (78%), Gaps = 2/317 (0%)
 Frame = -3

Query: 1142 CSKSPREFDLALTSKNAGFKLEGVEEEIRVFIQSDLDPGIQQ--TFQTDVYMGALSTNLF 969
            C KS  E D A   KN          ++ +F+QS++   ++   +F   ++M ++ST+ F
Sbjct: 12   CGKSSVETDAATRLKNNNVLKLPDNTKVSIFLQSEIKNLVKDDDSFNLSLFMNSISTHRF 71

Query: 968  GRLLIWSPRLPSTHDVVSQNFCEIPLGTVCVTDLQFKGRGRSKNVWESPPGCVMFSFTLE 789
            GR LIWSP L STHDVVS NF EIP+G+VCVTD+QFKGRGR+KNVWESP GC+M+SFT+E
Sbjct: 72   GRFLIWSPFLSSTHDVVSHNFSEIPVGSVCVTDIQFKGRGRTKNVWESPKGCLMYSFTVE 131

Query: 788  MEDGRKLPLLQYVVSLAITEAIKDVCRMQGLPELDVRIKWPNDLYLNGLKVGGILCTSTY 609
            MEDGR +PL+QYVVSLA+TEA+KDVC  +GL  +DV+IKWPNDLYLNGLKVGGILCTSTY
Sbjct: 132  MEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYIDVKIKWPNDLYLNGLKVGGILCTSTY 191

Query: 608  RSKKFHVCAGVGLNLDNEKPTTCLNAALKEMIPDSGQIKREDLVAAFFNKFENLFEVFLG 429
             S+KFHV  GVGLN+DNEKPTTCLNA LKE  P S  +KRE+++ AFF+KFE  F++F+ 
Sbjct: 192  GSRKFHVSVGVGLNVDNEKPTTCLNAVLKENCPASNLLKREEILGAFFHKFETFFDLFME 251

Query: 428  QGFQALEELYYQTWLHSGQKVVIEEKNNGHVENIAVIIQGLTPSGYLLATDEENKEFELH 249
            QGF++LEELYY+TWLHSGQ+V++EEKN   V    V IQGLT SGYLLA  ++N  +ELH
Sbjct: 252  QGFKSLEELYYRTWLHSGQRVIVEEKNEDQVVQNVVTIQGLTSSGYLLAIGDDNVMYELH 311

Query: 248  PDGNSFDFFKGLVRRKL 198
            PDGNSFDFFKGLVRRKL
Sbjct: 312  PDGNSFDFFKGLVRRKL 328