BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00006052
         (1599 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002277399.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   352   e-111
ref|XP_003543397.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   350   e-110
ref|XP_003540274.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   344   e-108
gb|ACU19018.1| unknown [Glycine max]                                  343   e-108
ref|XP_003596935.1| RING finger protein [Medicago truncatula] gi...   338   e-105

>ref|XP_002277399.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A [Vitis vinifera]
            gi|296084011|emb|CBI24399.3| unnamed protein product
            [Vitis vinifera]
          Length = 393

 Score =  352 bits (903), Expect(2) = e-111
 Identities = 190/320 (59%), Positives = 226/320 (70%), Gaps = 5/320 (1%)
 Frame = +2

Query: 272  HLQCILEWCQRSSQCPMCWQSISLKDPSSQELLEAVESERNIRSNQTRTATIFHHPAFGE 451
            HLQCILEWCQRSSQCPMCWQ ISLKDP+SQELLEAVE ER  R N +R ATIFHHP  G+
Sbjct: 58   HLQCILEWCQRSSQCPMCWQPISLKDPTSQELLEAVERERTFRFNPSRNATIFHHPTLGD 117

Query: 452  FDLQHLPVGVNDSELEERIIQHLXXXXXMGRAHHIGRRVVQRGRSGAQGHPQFLLFXXXX 631
            F+LQHLPVG ND+ELEERIIQHL     MGRA HI RR  QR RS AQG PQFL+F    
Sbjct: 118  FELQHLPVGANDAELEERIIQHLAAAAAMGRARHIARREGQRTRSSAQGRPQFLVFSAHP 177

Query: 632  XXXXXXXXXXXXXQRGEDEPTPAIFFASPSA-HTSGGEPPLR-ATNVTPAQSDQVNILPS 805
                         QRGEDEP PAI  A PS+  T+ GE   R  T+   +Q++++    S
Sbjct: 178  STPQAGPASSSPAQRGEDEPAPAITAAMPSSLPTAVGEESSRLTTSQLSSQAEEITASAS 237

Query: 806  GTTVGSFTRSGLSS-NMNSPGQSSPVNHDRAGPSEVQSVLESLKSRLNTVSMRYKESVTK 982
            G+ + +  + G SS N  SPGQS P   D+AGPSE QS  E+LKSR N VSMRYKES++K
Sbjct: 238  GSIIHTTNQHGTSSNNRRSPGQSLPSGQDKAGPSEFQSFSETLKSRFNAVSMRYKESISK 297

Query: 983  STRGWKERLFSRNSSVADLGSEVRREVNAGIATVSRLIERLDTRDGRRATAVPASPDDAG 1162
            STRGWKERLFSRN+S+AD+GS+VRREVNAGIATVSR++E L+TRD  R T  P+S  D+ 
Sbjct: 298  STRGWKERLFSRNTSMADIGSDVRREVNAGIATVSRMMEHLETRDNSRTT--PSSVSDSS 355

Query: 1163 RLSSSEPSI--ERVAENETN 1216
               +S PS+  +R+AE   +
Sbjct: 356  E-DTSSPSLGDQRMAETSAH 374



 Score = 78.2 bits (191), Expect(2) = e-111
 Identities = 35/45 (77%), Positives = 40/45 (88%)
 Frame = +3

Query: 45  MDEPMKLENHASSAAAFVEGGVQDACDDSCSICLEAFCDSDPSTV 179
           M+E  K E H +SAAAFVEGG+Q+ACDD+CSICLEAFCDSDPSTV
Sbjct: 6   MEESNKSECHLTSAAAFVEGGIQEACDDACSICLEAFCDSDPSTV 50


>ref|XP_003543397.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A-like [Glycine max]
          Length = 398

 Score =  350 bits (897), Expect(2) = e-110
 Identities = 184/320 (57%), Positives = 226/320 (70%), Gaps = 3/320 (0%)
 Frame = +2

Query: 272  HLQCILEWCQRSSQCPMCWQSISLKDPSSQELLEAVESERNIRSNQTRTATIFHHPAFGE 451
            HLQCILEWCQRSSQCPMCWQ ISLKDP+SQELLEAVE ERN R N +R ATIFHHP  G+
Sbjct: 64   HLQCILEWCQRSSQCPMCWQPISLKDPTSQELLEAVERERNFRFNPSRNATIFHHPTLGD 123

Query: 452  FDLQHLPVGVNDSELEERIIQHLXXXXXMGRAHHIGRRVVQRGRSGAQGHPQFLLFXXXX 631
            F+LQHLPVG +D++LEERIIQHL     MGRA H+ RR  QR RS AQG   FL+F    
Sbjct: 124  FELQHLPVGASDADLEERIIQHLAAAAAMGRARHVARREGQRNRSSAQGRQHFLVFSTHP 183

Query: 632  XXXXXXXXXXXXXQRGEDEPTPAIFFASPSAHTSGGEPPLRATNVTPAQSDQVNILPSGT 811
                         QRG+DEPTP I  A+ S   + GE   + T V+P Q++QV+   SGT
Sbjct: 184  NSPPMAPASSSPSQRGDDEPTPTIDVANLSPTPATGEESSQPTLVSPVQAEQVSASGSGT 243

Query: 812  TVGSFTRSGLS-SNMNSPGQSSPVNHDRAGPSEVQSVLESLKSRLNTVSMRYKESVTKST 988
            T  +    G S +N  SP QSSP + DRAGPSE+QS  ESLKS+LN VS RYKES++KST
Sbjct: 244  TALATDHQGSSYNNRRSPNQSSPSSQDRAGPSELQSFSESLKSKLNAVSTRYKESISKST 303

Query: 989  RGWKERLFSRNSSVADLGSEVRREVNAGIATVSRLIERLDTRDGRR--ATAVPASPDDAG 1162
            RGWKER FSRN++++DLGSEVRREVNAGIATVSR++ERL+TRD  R  + + P++ +D  
Sbjct: 304  RGWKERWFSRNNTMSDLGSEVRREVNAGIATVSRMMERLETRDNNRSNSNSAPSNIEDGS 363

Query: 1163 RLSSSEPSIERVAENETNTI 1222
               S++ ++   A NE  ++
Sbjct: 364  VQGSNDQTL--TAGNEGGSL 381



 Score = 77.0 bits (188), Expect(2) = e-110
 Identities = 34/45 (75%), Positives = 40/45 (88%)
 Frame = +3

Query: 45  MDEPMKLENHASSAAAFVEGGVQDACDDSCSICLEAFCDSDPSTV 179
           +++  K E H +SAAAFVEGG+QDACDD+CSICLEAFCDSDPSTV
Sbjct: 12  INDDNKSEAHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTV 56


>ref|XP_003540274.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A-like [Glycine max]
          Length = 401

 Score =  344 bits (883), Expect(2) = e-108
 Identities = 179/298 (60%), Positives = 214/298 (71%), Gaps = 3/298 (1%)
 Frame = +2

Query: 272  HLQCILEWCQRSSQCPMCWQSISLKDPSSQELLEAVESERNIRSNQTRTATIFHHPAFGE 451
            HLQCILEWCQRSSQCPMCWQ ISLKDP+SQELLEAVE ERN R N +R ATIFHHP  G+
Sbjct: 67   HLQCILEWCQRSSQCPMCWQPISLKDPTSQELLEAVERERNFRFNPSRNATIFHHPTLGD 126

Query: 452  FDLQHLPVGVNDSELEERIIQHLXXXXXMGRAHHIGRRVVQRGRSGAQGHPQFLLFXXXX 631
            F+LQHLPVG +D++LEERIIQHL     MGRA HI RR  QR RS AQG P FL+F    
Sbjct: 127  FELQHLPVGASDADLEERIIQHLAAAAAMGRARHIARREGQRNRSSAQGRPHFLVFSTNP 186

Query: 632  XXXXXXXXXXXXXQRGEDEPTPAIFFASPSAHTSGGEPPLRATNVTPAQSDQVNILPSGT 811
                         QRG+DEP P I  A+ S   + GE   + T V P Q++QV+   SG+
Sbjct: 187  NSPPMAPASSSPSQRGDDEPIPTIAVANLSPTPATGEESSQLTLVPPIQAEQVSASGSGS 246

Query: 812  TVGSFTRSGLS-SNMNSPGQSSPVNHDRAGPSEVQSVLESLKSRLNTVSMRYKESVTKST 988
            T  +    G S +N  SP QSSP + DRAGPSE+QS  ESLKS+LN VS RYKES++KST
Sbjct: 247  TALATDHQGSSYNNRRSPNQSSPSSQDRAGPSELQSFSESLKSKLNAVSTRYKESISKST 306

Query: 989  RGWKERLFSRNSSVADLGSEVRREVNAGIATVSRLIERLDTRDGRR--ATAVPASPDD 1156
            RGWKER FSRN++++DLGSEVRREVNAGIATVSR++ERL+T D  R  + + P++ +D
Sbjct: 307  RGWKERWFSRNNTMSDLGSEVRREVNAGIATVSRMMERLETSDNNRSNSNSAPSNIED 364



 Score = 77.4 bits (189), Expect(2) = e-108
 Identities = 35/44 (79%), Positives = 38/44 (86%)
 Frame = +3

Query: 48  DEPMKLENHASSAAAFVEGGVQDACDDSCSICLEAFCDSDPSTV 179
           D   K E H +SAAAFVEGG+QDACDD+CSICLEAFCDSDPSTV
Sbjct: 16  DNNNKSEAHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTV 59


>gb|ACU19018.1| unknown [Glycine max]
          Length = 401

 Score =  343 bits (881), Expect(2) = e-108
 Identities = 179/298 (60%), Positives = 213/298 (71%), Gaps = 3/298 (1%)
 Frame = +2

Query: 272  HLQCILEWCQRSSQCPMCWQSISLKDPSSQELLEAVESERNIRSNQTRTATIFHHPAFGE 451
            HLQCILEWCQRSSQCPMCWQ ISLKDP+SQELLEAVE ERN R N +R ATIFHHP  G+
Sbjct: 67   HLQCILEWCQRSSQCPMCWQPISLKDPTSQELLEAVERERNFRFNPSRNATIFHHPTLGD 126

Query: 452  FDLQHLPVGVNDSELEERIIQHLXXXXXMGRAHHIGRRVVQRGRSGAQGHPQFLLFXXXX 631
            F+LQHLPVG +D++LEERIIQHL     MGRA HI RR  QR RS AQG P FL F    
Sbjct: 127  FELQHLPVGASDADLEERIIQHLAAAAAMGRARHIARREGQRNRSSAQGRPHFLAFSTNP 186

Query: 632  XXXXXXXXXXXXXQRGEDEPTPAIFFASPSAHTSGGEPPLRATNVTPAQSDQVNILPSGT 811
                         QRG+DEP P I  A+ S   + GE   + T V P Q++QV+   SG+
Sbjct: 187  NSPPMAPASSSPSQRGDDEPIPTIAVANLSPTPATGEESSQLTLVPPIQAEQVSASGSGS 246

Query: 812  TVGSFTRSGLS-SNMNSPGQSSPVNHDRAGPSEVQSVLESLKSRLNTVSMRYKESVTKST 988
            T  +    G S +N  SP QSSP + DRAGPSE+QS  ESLKS+LN VS RYKES++KST
Sbjct: 247  TALATDHQGSSYNNRRSPNQSSPSSQDRAGPSELQSFSESLKSKLNAVSTRYKESISKST 306

Query: 989  RGWKERLFSRNSSVADLGSEVRREVNAGIATVSRLIERLDTRDGRR--ATAVPASPDD 1156
            RGWKER FSRN++++DLGSEVRREVNAGIATVSR++ERL+T D  R  + + P++ +D
Sbjct: 307  RGWKERWFSRNNTMSDLGSEVRREVNAGIATVSRMMERLETSDNNRSNSNSAPSNIED 364



 Score = 77.4 bits (189), Expect(2) = e-108
 Identities = 35/44 (79%), Positives = 38/44 (86%)
 Frame = +3

Query: 48  DEPMKLENHASSAAAFVEGGVQDACDDSCSICLEAFCDSDPSTV 179
           D   K E H +SAAAFVEGG+QDACDD+CSICLEAFCDSDPSTV
Sbjct: 16  DNNNKSEAHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTV 59


>ref|XP_003596935.1| RING finger protein [Medicago truncatula] gi|355485983|gb|AES67186.1|
            RING finger protein [Medicago truncatula]
          Length = 383

 Score =  338 bits (867), Expect(2) = e-105
 Identities = 176/290 (60%), Positives = 206/290 (71%), Gaps = 2/290 (0%)
 Frame = +2

Query: 272  HLQCILEWCQRSSQCPMCWQSISLKDPSSQELLEAVESERNIRSNQTRTATIFHHPAFGE 451
            HLQCILEWCQRSSQCPMCWQSISLKDP+ QELLEAVE ERNIR N TR ATIFHHP  G+
Sbjct: 60   HLQCILEWCQRSSQCPMCWQSISLKDPTGQELLEAVERERNIRFNPTRNATIFHHPTLGD 119

Query: 452  FDLQHLPVGVNDSELEERIIQHLXXXXXMGRAHHIGRRVVQRGRSGAQGHPQFLLFXXXX 631
            F+LQHLPVG ND++LEERI+QH      MGRA HI RR  QR RS AQG PQ+++F    
Sbjct: 120  FELQHLPVGANDADLEERILQHFAAAAAMGRARHIARREGQRNRSSAQGRPQYMVFSAHP 179

Query: 632  XXXXXXXXXXXXXQRGEDEPTPAIFFASPSAHTSGGEPPLRATNVTPAQSDQVNILPSGT 811
                         QRG+ E TPA            GE P + T V P Q+DQV+   SG+
Sbjct: 180  NSPPMAPASSSPPQRGDGEQTPAT-----------GEEPPQLTLVPPVQTDQVSASGSGS 228

Query: 812  TVGSFTRSGLS--SNMNSPGQSSPVNHDRAGPSEVQSVLESLKSRLNTVSMRYKESVTKS 985
               +    GLS  S+  SP QSSP + DRAGPSE+QS  ESLKS+LN VS RYKES++KS
Sbjct: 229  AALATDNLGLSYNSSRRSPNQSSPSSQDRAGPSELQSFSESLKSKLNAVSTRYKESISKS 288

Query: 986  TRGWKERLFSRNSSVADLGSEVRREVNAGIATVSRLIERLDTRDGRRATA 1135
            TRGWKER FSR S ++DLGSEV+REVNAGIATVSR++ERL+TRD  R+++
Sbjct: 289  TRGWKERWFSRTSPMSDLGSEVKREVNAGIATVSRMMERLETRDNNRSSS 338



 Score = 72.8 bits (177), Expect(2) = e-105
 Identities = 37/51 (72%), Positives = 41/51 (80%)
 Frame = +3

Query: 27  EV*VLRMDEPMKLENHASSAAAFVEGGVQDACDDSCSICLEAFCDSDPSTV 179
           EV V+  +E  K E H +SAAAFVEGG+QDACDDSCSICLE F DSDPSTV
Sbjct: 3   EVPVIEENET-KSEAHLTSAAAFVEGGIQDACDDSCSICLEEFIDSDPSTV 52