BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00004851
         (1497 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002267490.2| PREDICTED: probable ubiquitin-conjugating en...   424   e-116
gb|ACB12194.1| PHO2 [Nicotiana benthamiana]                           406   e-111
ref|XP_002329823.1| predicted protein [Populus trichocarpa] gi|2...   405   e-110
ref|XP_003542749.1| PREDICTED: probable ubiquitin-conjugating en...   402   e-109
ref|XP_003528454.1| PREDICTED: probable ubiquitin-conjugating en...   402   e-109

>ref|XP_002267490.2| PREDICTED: probable ubiquitin-conjugating enzyme E2 24-like [Vitis
            vinifera] gi|297735992|emb|CBI23966.3| unnamed protein
            product [Vitis vinifera]
          Length = 944

 Score =  424 bits (1091), Expect = e-116
 Identities = 228/441 (51%), Positives = 284/441 (64%), Gaps = 29/441 (6%)
 Frame = +3

Query: 3    LDRLDDPPTAPTDNVEEISENISEQMVEQETLSWRKKDKVKEISLQDD-----AWDSGAL 167
            +D+ +   T P +N+EE++  + E   + ++   ++KD V ++++ D       W S + 
Sbjct: 499  IDKFEGSSTTPEENIEELNHELIED--DNQSSPGKEKD-VSDLNIVDKDCTKYPWQSTSS 555

Query: 168  LFPRAAICFLTNVAKSLFSSRGSTSLPGTWHELSTEEPQPDVGDLSTANLNP-------- 323
            L PRA I F  ++A SLF S  S  L G        E + + G      LN         
Sbjct: 556  LLPRAVIGFFLSIAASLFGSPDSALLSGQLSSSHCSEDENESGTHLKGVLNSCNPCTEEQ 615

Query: 324  -----PIQA------KIEQKE-----HPPVLPGNDKAGGFEKFVVVDDYSDHHFVMSNDK 455
                  +QA      K E KE       P   G+     F +F +V D SDHHFV S  K
Sbjct: 616  HIVVDDLQASGETSVKEEIKEIGGDKDLPFSSGSKNPEQFSQFDMVCDCSDHHFVDSAGK 675

Query: 456  RLAISQVKSGWLKKLHQEWSILESNLPDSIYVRVYEDRIDLLRAAIIGAPGTPYHDGLFF 635
             LA+SQVK GWLKK+ QEWS+LE NLP++IYVR+YE+R+DLLRAAI+G   TPYHDGLFF
Sbjct: 676  GLALSQVKIGWLKKVQQEWSMLEKNLPETIYVRIYEERMDLLRAAIVGPSATPYHDGLFF 735

Query: 636  FDICLPSDYPHEPPLVHYNSGGLRLNPNLYESGKVCLSLLNTWTGTRTEAWNPESSTIXX 815
            FDI LP +YPHEPPLVHYNSGGLR+NPNLYESGKVCLSLLNTWTGT TE WNP SSTI  
Sbjct: 736  FDIFLPPEYPHEPPLVHYNSGGLRVNPNLYESGKVCLSLLNTWTGTGTEVWNPGSSTILQ 795

Query: 816  XXXXXXXXXXNDKPYFNEAGYDKQVGKIEGEKNSITYNENAFLLSLKSMLYILNNPPKHF 995
                      N+KPYFNEAGYDKQ+G+ EGEKNSI+YNENAF+ + +S+LY+L NPPKHF
Sbjct: 796  VLLSLQALVLNEKPYFNEAGYDKQMGRAEGEKNSISYNENAFIGTCRSILYLLRNPPKHF 855

Query: 996  EPLVLEHFACRSHSILHACKAYMDGAQIGSDFNSEKPSGEILKGSSTGFKLMLAKLFPKL 1175
            E +V EHF   S   L ACKAYM+GA +G  F  +K + E  KGSSTGFK+MLAKLFPKL
Sbjct: 856  EAIVEEHFNQCSQHFLLACKAYMEGAPVGCAFEHKKTAEESQKGSSTGFKIMLAKLFPKL 915

Query: 1176 VSGFTDKGIDCGHFLNQVKGV 1238
            V  F+ +GIDC  F+   KG+
Sbjct: 916  VEAFSARGIDCSQFVEMEKGI 936


>gb|ACB12194.1| PHO2 [Nicotiana benthamiana]
          Length = 921

 Score =  406 bits (1043), Expect = e-111
 Identities = 212/376 (56%), Positives = 262/376 (69%), Gaps = 23/376 (6%)
 Frame = +3

Query: 150  WDSGALLFPRAAICFLTNVAKSLFSSRGSTSLPGTWHELSTEEPQP------DVGDLSTA 311
            W+S + L  +AAI   +++  SLF S  STSL GT+  +S E  +       +V +LS  
Sbjct: 543  WNSSSCLLSQAAIGSFSSITSSLFGSL-STSLFGTYQAISEEGQESRIPNEEEVIELSNL 601

Query: 312  NLNPP----------IQAKIEQKEHPP------VLPGNDKAG-GFEKFVVVDDYSDHHFV 440
            N   P          ++ ++EQ++          LP + K    F +F +V  +SDHHF 
Sbjct: 602  NAGIPTLEVGYVKASLEMELEQEQETTEDQKHYALPSSSKLPEDFRQFDMVTGFSDHHFA 661

Query: 441  MSNDKRLAISQVKSGWLKKLHQEWSILESNLPDSIYVRVYEDRIDLLRAAIIGAPGTPYH 620
                K   +SQV+ GWLKK+ QEWSILE +LP++IYVRVYE+R+DLLRAAIIGAPGTPYH
Sbjct: 662  DGAGKE-QLSQVRRGWLKKVQQEWSILEHDLPETIYVRVYEERMDLLRAAIIGAPGTPYH 720

Query: 621  DGLFFFDICLPSDYPHEPPLVHYNSGGLRLNPNLYESGKVCLSLLNTWTGTRTEAWNPES 800
            DGLFFFDI LPS+YPHEPP+V Y+SGGLR+NPNLYESGKVCLSLLNTWTG+  E WNP+S
Sbjct: 721  DGLFFFDIYLPSNYPHEPPMVSYHSGGLRVNPNLYESGKVCLSLLNTWTGSGNEVWNPKS 780

Query: 801  STIXXXXXXXXXXXXNDKPYFNEAGYDKQVGKIEGEKNSITYNENAFLLSLKSMLYILNN 980
            STI            N+KPYFNEAGYD Q+GK +GEKNS++YNENAFL++ KSMLY LN 
Sbjct: 781  STILQVLLSFQALVLNEKPYFNEAGYDAQIGKADGEKNSVSYNENAFLVTCKSMLYQLNK 840

Query: 981  PPKHFEPLVLEHFACRSHSILHACKAYMDGAQIGSDFNSEKPSGEILKGSSTGFKLMLAK 1160
            PPKHFE LV EHF  R   IL ACKAYMDGA +GS F  +    E+++GSSTGFK+ML K
Sbjct: 841  PPKHFEALVQEHFGKRWKHILLACKAYMDGAPVGSAFQPKSQEQELIEGSSTGFKIMLGK 900

Query: 1161 LFPKLVSGFTDKGIDC 1208
            LFPKL++ FT KGIDC
Sbjct: 901  LFPKLMAAFTGKGIDC 916


>ref|XP_002329823.1| predicted protein [Populus trichocarpa] gi|222870885|gb|EEF08016.1|
            predicted protein [Populus trichocarpa]
          Length = 924

 Score =  405 bits (1041), Expect = e-110
 Identities = 218/425 (51%), Positives = 272/425 (64%), Gaps = 38/425 (8%)
 Frame = +3

Query: 72   EQMVEQETLSWRK-------KDKVKEISLQDDA----WDSGAL-LFPRAAICFLTNVAKS 215
            E+ V QET+   K       KD +  IS+ D++    W+S +  L P++A+ F + +   
Sbjct: 501  EEEVNQETVDHDKQFSVLKGKDLLNSISIGDESTKCPWESSSFSLLPQSALGFFSRITGG 560

Query: 216  LFSSRGSTSLPGT---------WHELST--EEPQPDVGDLST---------------ANL 317
            +F   GSTS+ G           +E  T  E+  P+  DL                  NL
Sbjct: 561  IFGPFGSTSVSGPVASDLISEDGNEFKTPEEKENPEACDLCMEMQPLVAGDMLRFEGTNL 620

Query: 318  NPPIQAKIEQKEHPPVLPGNDKAGGFEKFVVVDDYSDHHFVMSNDKRLAISQVKSGWLKK 497
               I    E KEH      + +   F++F +V   SDHHF+       A+SQVK GWL+K
Sbjct: 621  KLEINDDQESKEHRSS-SASKRPEPFDQFDMVAVCSDHHFLDGAGNVPALSQVKRGWLRK 679

Query: 498  LHQEWSILESNLPDSIYVRVYEDRIDLLRAAIIGAPGTPYHDGLFFFDICLPSDYPHEPP 677
            + QEWSILE NLP+SIYVR+YEDR+DLLRAAI+G+ GTPYHDGLFFFDI LP  YPHEPP
Sbjct: 680  VQQEWSILEKNLPESIYVRIYEDRMDLLRAAIVGSNGTPYHDGLFFFDIFLPPGYPHEPP 739

Query: 678  LVHYNSGGLRLNPNLYESGKVCLSLLNTWTGTRTEAWNPESSTIXXXXXXXXXXXXNDKP 857
            LVHY+SGGLR+NPNLYESGK+CLSLLNTWTGT +E WNPESS+I            N+KP
Sbjct: 740  LVHYHSGGLRVNPNLYESGKICLSLLNTWTGTGSEVWNPESSSILQVLLSLQALVLNEKP 799

Query: 858  YFNEAGYDKQVGKIEGEKNSITYNENAFLLSLKSMLYILNNPPKHFEPLVLEHFACRSHS 1037
            YFNEAGYDKQ+G+ EGEKNSI+YNENAFL++ KSMLY+L  PPKHFEPL+ EH   RS +
Sbjct: 800  YFNEAGYDKQIGRAEGEKNSISYNENAFLMTWKSMLYLLRQPPKHFEPLIEEHLKLRSQN 859

Query: 1038 ILHACKAYMDGAQIGSDFNSEKPSGEILKGSSTGFKLMLAKLFPKLVSGFTDKGIDCGHF 1217
            IL ACK+Y++GA +    +S     E  KG STGFK+ML KLFPKLV  F+ KGIDC  F
Sbjct: 860  ILLACKSYLEGAPVAYALDSGSTEHENQKGGSTGFKIMLGKLFPKLVETFSGKGIDCSRF 919

Query: 1218 LNQVK 1232
              + K
Sbjct: 920  TEEEK 924


>ref|XP_003542749.1| PREDICTED: probable ubiquitin-conjugating enzyme E2 24-like [Glycine
            max]
          Length = 933

 Score =  402 bits (1032), Expect = e-109
 Identities = 212/428 (49%), Positives = 275/428 (64%), Gaps = 25/428 (5%)
 Frame = +3

Query: 24   PTAPTDNVEEISENISEQMV---EQETLSWRKKDKVKEISLQDDAWDSGALLFPRAAICF 194
            PT    NVEE +  I E      +Q+       D  +E + + +  +  +   PRAA   
Sbjct: 501  PTPFETNVEEFTHEIIEHRSLPSDQKGKDLLNGDGTRE-NCEKNLGECSSFSLPRAAFEL 559

Query: 195  LTNVAKSLFSSRGSTSLPGTWHELSTEEPQP-----DVGDLSTANL-------------- 317
             +++  S+F +   T + G    + T E +      D  DL T++L              
Sbjct: 560  FSSIKASIFQTFRGTLISGAVSSVPTFEKKNGYDCLDKKDLETSDLFTEQHPMAELQYTE 619

Query: 318  ---NPPIQAKIEQKEHPPVLPGNDKAGGFEKFVVVDDYSDHHFVMSNDKRLAISQVKSGW 488
               + P   +I +K   P    ++ +  F++F V+++  DHHF +   K L+ SQVK  W
Sbjct: 620  DKTSYPENIEIHEKNDFPFSLDSNSSNQFKQFDVIENCPDHHFFVEG-KGLSTSQVKRSW 678

Query: 489  LKKLHQEWSILESNLPDSIYVRVYEDRIDLLRAAIIGAPGTPYHDGLFFFDICLPSDYPH 668
            +KK+ QEWSILE NLP++IYVRV+E+R+DL+RAAI+GA GTPYHDGLFFFDIC P +YP+
Sbjct: 679  VKKVQQEWSILEKNLPETIYVRVFEERMDLMRAAIVGASGTPYHDGLFFFDICFPPEYPN 738

Query: 669  EPPLVHYNSGGLRLNPNLYESGKVCLSLLNTWTGTRTEAWNPESSTIXXXXXXXXXXXXN 848
            EPP+VHYNS GLRLNPNLYESGK+CLSLLNTWTGT TE WNP +STI            N
Sbjct: 739  EPPMVHYNSAGLRLNPNLYESGKICLSLLNTWTGTDTEVWNPGASTILQVLLSLQALVLN 798

Query: 849  DKPYFNEAGYDKQVGKIEGEKNSITYNENAFLLSLKSMLYILNNPPKHFEPLVLEHFACR 1028
            +KPYFNEAGYD+Q+G+ EGEKNS++YNENAFL++ KSM+Y+L  PPKHFE LV EHF   
Sbjct: 799  EKPYFNEAGYDQQIGRAEGEKNSVSYNENAFLVTTKSMMYLLRKPPKHFEALVEEHFRKC 858

Query: 1029 SHSILHACKAYMDGAQIGSDFNSEKPSGEILKGSSTGFKLMLAKLFPKLVSGFTDKGIDC 1208
            S  IL ACKAY++GA IG  F S K   E  KG+STGFK+MLAKLFPKLV  F+DKGIDC
Sbjct: 859  SQHILLACKAYLEGASIGCAFGSGKTGHENQKGTSTGFKIMLAKLFPKLVEAFSDKGIDC 918

Query: 1209 GHFLNQVK 1232
              F++  K
Sbjct: 919  SQFVDLQK 926


>ref|XP_003528454.1| PREDICTED: probable ubiquitin-conjugating enzyme E2 24-like [Glycine
            max]
          Length = 927

 Score =  402 bits (1032), Expect = e-109
 Identities = 213/433 (49%), Positives = 278/433 (64%), Gaps = 30/433 (6%)
 Frame = +3

Query: 24   PTAPTDNVEEISENISEQMVEQETLSWRKKDKVKEI--------SLQDDAWDSGALLFPR 179
            PT    NVEE++     +M+E  +L    K K K++        + + +  +  +   PR
Sbjct: 501  PTPYETNVEELTH----EMIEHRSLPSSDK-KGKDLLNGDGTRKNCEKNLGECSSFSLPR 555

Query: 180  AAICFLTNVAKSLFSSRGSTSLPGTWHELSTEEPQ-----PDVGDLSTANL--------- 317
            AA    +++  S+F +   T L G    + T E +     PD  D  T +L         
Sbjct: 556  AAFELFSSIKASIFQTFSGTLLSGAVSSVPTFEKENGYAYPDKKDSETCDLFTEQHPMTE 615

Query: 318  --------NPPIQAKIEQKEHPPVLPGNDKAGGFEKFVVVDDYSDHHFVMSNDKRLAISQ 473
                    + P   KI +K   P    ++ +  F++F V ++  DHHF +   K L+ISQ
Sbjct: 616  LQYTEDKTSYPENIKIHEKNDFPFSLDSNSSNQFKQFDVKENCPDHHFFVQG-KGLSISQ 674

Query: 474  VKSGWLKKLHQEWSILESNLPDSIYVRVYEDRIDLLRAAIIGAPGTPYHDGLFFFDICLP 653
            VK  W+KK+ QEWSILE NLP++IYV  +E+R+DL+RAAI+GA GTPYH+GLFFFDIC P
Sbjct: 675  VKRSWVKKVQQEWSILEKNLPETIYVCAFEERMDLMRAAIVGASGTPYHEGLFFFDICFP 734

Query: 654  SDYPHEPPLVHYNSGGLRLNPNLYESGKVCLSLLNTWTGTRTEAWNPESSTIXXXXXXXX 833
             +YP++PP+VHYNSGGLRLNPNLYESGK+CLSLLNTWTGT TE WNP +STI        
Sbjct: 735  PEYPNKPPMVHYNSGGLRLNPNLYESGKICLSLLNTWTGTGTEVWNPGASTILQVLLSLQ 794

Query: 834  XXXXNDKPYFNEAGYDKQVGKIEGEKNSITYNENAFLLSLKSMLYILNNPPKHFEPLVLE 1013
                N+KPYFNEAGYD+Q+G+ EGEKNS++YNENA L++ KSM+Y+L  PPKHFE LV E
Sbjct: 795  ALVLNEKPYFNEAGYDQQIGRAEGEKNSVSYNENALLVTTKSMMYLLRKPPKHFEELVEE 854

Query: 1014 HFACRSHSILHACKAYMDGAQIGSDFNSEKPSGEILKGSSTGFKLMLAKLFPKLVSGFTD 1193
            HF  R   IL ACKAY++GA IG  F+SEK   E  KG+STGFK+MLAKLFPKLV  F+D
Sbjct: 855  HFRKRYQHILLACKAYLEGASIGCAFDSEKNEHENQKGTSTGFKIMLAKLFPKLVEAFSD 914

Query: 1194 KGIDCGHFLNQVK 1232
            KGIDC  F++  K
Sbjct: 915  KGIDCSQFVDLQK 927