BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00004438
         (468 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004138192.1| PREDICTED: flavonoid 3'-monooxygenase-like [...   183   1e-44
dbj|BAH22519.1| flavonoid 3' hydroxylase [Tricyrtis hirta]            175   4e-42
gb|AAV74194.1| flavonoid 3'-hydroxylase [Sorghum bicolor] gi|561...   173   2e-41
ref|XP_002452380.1| hypothetical protein SORBIDRAFT_04g024710 [S...   173   2e-41
gb|AFP95893.1| F3'H [Narcissus tazetta]                               172   2e-41

>ref|XP_004138192.1| PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis sativus]
           gi|449477168|ref|XP_004154949.1| PREDICTED: flavonoid
           3'-monooxygenase-like [Cucumis sativus]
          Length = 522

 Score =  183 bits (465), Expect = 1e-44
 Identities = 92/159 (57%), Positives = 114/159 (71%), Gaps = 6/159 (3%)
 Frame = -3

Query: 466 FNISDFIPWLKPLDILGLVGRMKRVHNWFDDLINKIIEE------RKMEGGRADFLSVLL 305
           FN  DF PWL+ LD++G+   MK+VH WFDD + KI+EE      R + GG  D LS LL
Sbjct: 220 FNPGDFFPWLEWLDLMGVGREMKKVHKWFDDFLTKIVEEHRNLLARGVGGGHQDLLSTLL 279

Query: 304 QQKENPSGDQETKLTDANIKALLLDMFLGGTDTTSSTVEWALVELIRHPDILAEVQKELD 125
             K++   D+  KL D  IKALLL+MF  GTDT++STVEWAL ELIRHP+++A+ Q+ELD
Sbjct: 280 SMKDDGE-DENEKLNDTEIKALLLNMFTAGTDTSASTVEWALTELIRHPEMMAQAQQELD 338

Query: 124 SIVGRSRLVKDVDLVNVPVLQAVIKETFRLHPPVPLLIP 8
           SIVGR R V DVDL  +  LQAV+KETFRLHPP PL +P
Sbjct: 339 SIVGRDRAVSDVDLHQLVYLQAVVKETFRLHPPTPLSLP 377


>dbj|BAH22519.1| flavonoid 3' hydroxylase [Tricyrtis hirta]
          Length = 505

 Score =  175 bits (443), Expect = 4e-42
 Identities = 84/153 (54%), Positives = 111/153 (72%)
 Frame = -3

Query: 466 FNISDFIPWLKPLDILGLVGRMKRVHNWFDDLINKIIEERKMEGGRADFLSVLLQQKENP 287
           FNI DF+P +  +D+ G+VG+MK++H   D  ++K+I E K   G  D LSVL+  K++ 
Sbjct: 212 FNIGDFVPGIGWMDLQGVVGKMKKLHKRMDAFLDKLINEHKRGSGGRDLLSVLVSLKDDE 271

Query: 286 SGDQETKLTDANIKALLLDMFLGGTDTTSSTVEWALVELIRHPDILAEVQKELDSIVGRS 107
             D+  KL+   IKALLL++F  GTDTTSSTVEWAL ELIRHP +L + Q+ELD++VGR 
Sbjct: 272 GEDEANKLSHTEIKALLLNLFTAGTDTTSSTVEWALAELIRHPTLLHKAQQELDNVVGRQ 331

Query: 106 RLVKDVDLVNVPVLQAVIKETFRLHPPVPLLIP 8
           RLV + DL N+P LQA+IKETFRLHP  PL +P
Sbjct: 332 RLVSETDLPNLPFLQAIIKETFRLHPSTPLSLP 364


>gb|AAV74194.1| flavonoid 3'-hydroxylase [Sorghum bicolor]
           gi|56121717|gb|AAV74195.1| flavonoid 3'-hydroxylase
           [Sorghum bicolor]
          Length = 517

 Score =  173 bits (438), Expect = 2e-41
 Identities = 84/156 (53%), Positives = 118/156 (75%), Gaps = 3/156 (1%)
 Frame = -3

Query: 466 FNISDFIPWLKPLDILGLVGRMKRVHNWFDDLINKIIEERKMEGGRADFLSVLL---QQK 296
           FN+ DF+P L  LD+ G+VG+MKR+H  +DD++N II ERK      D LSVLL   +++
Sbjct: 223 FNVGDFVPALARLDLQGVVGKMKRLHRRYDDMMNGIIRERKAAEEGKDLLSVLLARTREQ 282

Query: 295 ENPSGDQETKLTDANIKALLLDMFLGGTDTTSSTVEWALVELIRHPDILAEVQKELDSIV 116
           ++ +  +++++T+  IKALLL++F  GTDTTSSTVEWAL ELIRHPD+L + Q+ELD++V
Sbjct: 283 QSIADGEDSRITETEIKALLLNLFTAGTDTTSSTVEWALAELIRHPDVLKKAQEELDAVV 342

Query: 115 GRSRLVKDVDLVNVPVLQAVIKETFRLHPPVPLLIP 8
           GR+RLV ++DL  +  L AVIKETFR+HP  PL +P
Sbjct: 343 GRNRLVSELDLPRLTYLTAVIKETFRMHPSTPLSLP 378


>ref|XP_002452380.1| hypothetical protein SORBIDRAFT_04g024710 [Sorghum bicolor]
           gi|241932211|gb|EES05356.1| hypothetical protein
           SORBIDRAFT_04g024710 [Sorghum bicolor]
          Length = 517

 Score =  173 bits (438), Expect = 2e-41
 Identities = 84/156 (53%), Positives = 118/156 (75%), Gaps = 3/156 (1%)
 Frame = -3

Query: 466 FNISDFIPWLKPLDILGLVGRMKRVHNWFDDLINKIIEERKMEGGRADFLSVLL---QQK 296
           FN+ DF+P L  LD+ G+VG+MKR+H  +DD++N II ERK      D LSVLL   +++
Sbjct: 223 FNVGDFVPALARLDLQGVVGKMKRLHRRYDDMMNGIIRERKAAEEGKDLLSVLLARTREQ 282

Query: 295 ENPSGDQETKLTDANIKALLLDMFLGGTDTTSSTVEWALVELIRHPDILAEVQKELDSIV 116
           ++ +  +++++T+  IKALLL++F  GTDTTSSTVEWAL ELIRHPD+L + Q+ELD++V
Sbjct: 283 QSIADGEDSRITETEIKALLLNLFTAGTDTTSSTVEWALAELIRHPDVLKKAQEELDAVV 342

Query: 115 GRSRLVKDVDLVNVPVLQAVIKETFRLHPPVPLLIP 8
           GR+RLV ++DL  +  L AVIKETFR+HP  PL +P
Sbjct: 343 GRNRLVSELDLPRLTYLTAVIKETFRMHPSTPLSLP 378


>gb|AFP95893.1| F3'H [Narcissus tazetta]
          Length = 528

 Score =  172 bits (437), Expect = 2e-41
 Identities = 85/155 (54%), Positives = 110/155 (70%), Gaps = 1/155 (0%)
 Frame = -3

Query: 466 FNISDFIPWLKPLDILGLVGRMKRVHNWFDDLINKIIEERKMEGGRAD-FLSVLLQQKEN 290
           FNI DF+PWL+P D+ G+VG+MK VH  FD  + K IEE +    + D  LSVL++  E+
Sbjct: 237 FNICDFLPWLRPFDLQGVVGKMKNVHRRFDTFLGKAIEEHRRADAKGDNLLSVLIRLMED 296

Query: 289 PSGDQETKLTDANIKALLLDMFLGGTDTTSSTVEWALVELIRHPDILAEVQKELDSIVGR 110
                  +LT+ +IKALLLD+F+ GTDT SSTVEWAL ELI HP+IL + Q ELDS+ G 
Sbjct: 297 TES-HGGELTNTSIKALLLDLFIAGTDTASSTVEWALAELIGHPEILKKAQTELDSVAGS 355

Query: 109 SRLVKDVDLVNVPVLQAVIKETFRLHPPVPLLIPH 5
           +RLV + DL N+P L A++KETFRLHP  PL +PH
Sbjct: 356 NRLVSEEDLPNLPFLHAIVKETFRLHPSTPLSLPH 390