BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00004001
         (905 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002319761.1| flavonoid 3'-hydroxylase [Populus trichocarp...   316   4e-84
ref|NP_001064338.1| Os10g0320100 [Oryza sativa Japonica Group] g...   300   4e-79
gb|AFI71898.1| flavonoid 3'-hydroxylase [Paeonia lactiflora]          296   3e-78
dbj|BAH22519.1| flavonoid 3' hydroxylase [Tricyrtis hirta]            296   4e-78
gb|AGC29948.1| CYP75B65 [Sinopodophyllum hexandrum]                   294   2e-77

>ref|XP_002319761.1| flavonoid 3'-hydroxylase [Populus trichocarpa]
            gi|222858137|gb|EEE95684.1| flavonoid 3'-hydroxylase
            [Populus trichocarpa]
          Length = 521

 Score =  316 bits (810), Expect = 4e-84
 Identities = 158/263 (60%), Positives = 184/263 (69%)
 Frame = -3

Query: 903  IEEHKTRPKIESEAEAEGKDFLSVLLGLKEADIKDEETDNKLTDIDIKALXXXXXXXXXX 724
            +EEHKT     S    +  D LS L+ LKE    D E + KLTD +IKAL          
Sbjct: 256  VEEHKTSSSTASVRSEKHTDLLSTLIALKEQQDVDGE-EGKLTDTEIKALLLNMFTAGTD 314

Query: 723  XXXXTVEWALVELIRHPDILAAAQKELDSIVGRSRLVSELDLNNVPVLQAIIKETFRLHP 544
                TVEWA+ ELIRHPDILA  ++ELDS+VGR RLV+ELDL  +  LQA++KETFRLHP
Sbjct: 315  TSSSTVEWAIAELIRHPDILAQVKQELDSVVGRDRLVTELDLAQLTYLQAVVKETFRLHP 374

Query: 543  PVPLLIPHTAAEACEVAGYHIPKAATLLVNTWTICRDPDVWSRPLEFDPSRFLPGAKHAD 364
              PL +P  AAE+CE+ GYHIPK +T+LVN W I RDPDVW++PLEF P RFLPG   AD
Sbjct: 375  STPLSLPRIAAESCEIGGYHIPKGSTVLVNVWAIARDPDVWTKPLEFRPERFLPGGDKAD 434

Query: 363  VDVKGSHFELIPFGAGRRICLGMRLGLRMTTFLLASLVHGFDWALPDGLTPETLNMDVEF 184
            VDVKG+ FELIPFGAGRRIC GM LGLRM   L A+L+H FDW L DGL PE LNMD  +
Sbjct: 435  VDVKGNDFELIPFGAGRRICAGMSLGLRMVQLLTATLIHAFDWDLADGLVPEKLNMDEAY 494

Query: 183  GLTLERAVPLVARPIPRLDPKAY 115
            GLTL+RA PL+  P PRL PK Y
Sbjct: 495  GLTLQRADPLMVHPRPRLSPKVY 517


>ref|NP_001064338.1| Os10g0320100 [Oryza sativa Japonica Group]
            gi|19881547|gb|AAM00948.1|AC021892_12 Putative flavonoid
            3'-hydroxylase [Oryza sativa Japonica Group]
            gi|22725927|gb|AAN04937.1| Putative chalcone flavonoid 3'
            - hydroxylase [Oryza sativa Japonica Group]
            gi|31431083|gb|AAP52914.1| Flavonoid 3'-monooxygenase,
            putative, expressed [Oryza sativa Japonica Group]
            gi|113638947|dbj|BAF26252.1| Os10g0320100 [Oryza sativa
            Japonica Group] gi|125531442|gb|EAY78007.1| hypothetical
            protein OsI_33047 [Oryza sativa Indica Group]
            gi|125574353|gb|EAZ15637.1| hypothetical protein
            OsJ_31048 [Oryza sativa Japonica Group]
            gi|215693977|dbj|BAG89180.1| unnamed protein product
            [Oryza sativa Japonica Group] gi|340343700|gb|AEK31169.1|
            flavonoid 3'-hydroxylase [Oryza sativa Japonica Group]
          Length = 526

 Score =  300 bits (767), Expect = 4e-79
 Identities = 154/266 (57%), Positives = 181/266 (68%), Gaps = 1/266 (0%)
 Frame = -3

Query: 903  IEEHKTRPKIESEAEAE-GKDFLSVLLGLKEADIKDEETDNKLTDIDIKALXXXXXXXXX 727
            I E K   + +  A  E G D LSVLL   + + K +    K+T+ DIKAL         
Sbjct: 261  INERKAGAQPDGVAAGEHGNDLLSVLLARMQEEQKLDGDGEKITETDIKALLLNLFTAGT 320

Query: 726  XXXXXTVEWALVELIRHPDILAAAQKELDSIVGRSRLVSELDLNNVPVLQAIIKETFRLH 547
                 TVEWAL ELIRHPD+L  AQ ELD++VGR RLVSE DL  +P L A+IKETFRLH
Sbjct: 321  DTTSSTVEWALAELIRHPDVLKEAQHELDTVVGRGRLVSESDLPRLPYLTAVIKETFRLH 380

Query: 546  PPVPLLIPHTAAEACEVAGYHIPKAATLLVNTWTICRDPDVWSRPLEFDPSRFLPGAKHA 367
            P  PL +P  AAE CEV GY IPK ATLLVN W I RDP  W  PL++ PSRFLPG  HA
Sbjct: 381  PSTPLSLPREAAEECEVDGYRIPKGATLLVNVWAIARDPTQWPDPLQYQPSRFLPGRMHA 440

Query: 366  DVDVKGSHFELIPFGAGRRICLGMRLGLRMTTFLLASLVHGFDWALPDGLTPETLNMDVE 187
            DVDVKG+ F LIPFGAGRRIC G+  GLRM T + A+LVHGFDW L +G TP+ LNM+  
Sbjct: 441  DVDVKGADFGLIPFGAGRRICAGLSWGLRMVTLMTATLVHGFDWTLANGATPDKLNMEEA 500

Query: 186  FGLTLERAVPLVARPIPRLDPKAYVV 109
            +GLTL+RAVPL+ +P+PRL P AY V
Sbjct: 501  YGLTLQRAVPLMVQPVPRLLPSAYGV 526


>gb|AFI71898.1| flavonoid 3'-hydroxylase [Paeonia lactiflora]
          Length = 507

 Score =  296 bits (759), Expect = 3e-78
 Identities = 149/263 (56%), Positives = 185/263 (70%)
 Frame = -3

Query: 903  IEEHKTRPKIESEAEAEGKDFLSVLLGLKEADIKDEETDNKLTDIDIKALXXXXXXXXXX 724
            +EEHK    + S+   +  DFLS L+ LK+ D+  E    KLTDI+IKAL          
Sbjct: 252  LEEHK----MNSDGVKQQNDFLSKLISLKD-DVDGE--GGKLTDIEIKALLLNMFTAGTD 304

Query: 723  XXXXTVEWALVELIRHPDILAAAQKELDSIVGRSRLVSELDLNNVPVLQAIIKETFRLHP 544
                TVEW + ELIRHP ILA AQ+E+DS+VGR RLV+ELDL N+P LQA++KETFRLHP
Sbjct: 305  TSSSTVEWGIAELIRHPKILAQAQQEIDSVVGRDRLVTELDLPNLPFLQAVVKETFRLHP 364

Query: 543  PVPLLIPHTAAEACEVAGYHIPKAATLLVNTWTICRDPDVWSRPLEFDPSRFLPGAKHAD 364
              PL +P  A+++CE+ GY+IPK +TLLVN W I RDP+VW+ PLEF P RFLPG +  +
Sbjct: 365  STPLSLPRMASQSCEINGYYIPKGSTLLVNVWAIARDPNVWAEPLEFRPDRFLPGGEKPN 424

Query: 363  VDVKGSHFELIPFGAGRRICLGMRLGLRMTTFLLASLVHGFDWALPDGLTPETLNMDVEF 184
            +D+KG+ FE+IPFGAGRRIC GM LGLRM   L A+LVH FDW LP+G  PE L M+  +
Sbjct: 425  IDIKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTATLVHAFDWGLPEGQIPEKLQMEEAY 484

Query: 183  GLTLERAVPLVARPIPRLDPKAY 115
            GLTL+RAVPLV  P PRL    Y
Sbjct: 485  GLTLQRAVPLVLYPQPRLSSHVY 507


>dbj|BAH22519.1| flavonoid 3' hydroxylase [Tricyrtis hirta]
          Length = 505

 Score =  296 bits (758), Expect = 4e-78
 Identities = 150/255 (58%), Positives = 184/255 (72%)
 Frame = -3

Query: 873  ESEAEAEGKDFLSVLLGLKEADIKDEETDNKLTDIDIKALXXXXXXXXXXXXXXTVEWAL 694
            E +  + G+D LSVL+ LK+ + +DE   NKL+  +IKAL              TVEWAL
Sbjct: 250  EHKRGSGGRDLLSVLVSLKDDEGEDEA--NKLSHTEIKALLLNLFTAGTDTTSSTVEWAL 307

Query: 693  VELIRHPDILAAAQKELDSIVGRSRLVSELDLNNVPVLQAIIKETFRLHPPVPLLIPHTA 514
             ELIRHP +L  AQ+ELD++VGR RLVSE DL N+P LQAIIKETFRLHP  PL +P  +
Sbjct: 308  AELIRHPTLLHKAQQELDNVVGRQRLVSETDLPNLPFLQAIIKETFRLHPSTPLSLPRIS 367

Query: 513  AEACEVAGYHIPKAATLLVNTWTICRDPDVWSRPLEFDPSRFLPGAKHADVDVKGSHFEL 334
            +E CE+ GY+IPK ATLLVN W I RDP VWS PLEF P RF+PG + A+VDVKG+ FE+
Sbjct: 368  SEPCEINGYYIPKNATLLVNVWAIARDPAVWSDPLEFKPERFMPGGEKANVDVKGNDFEV 427

Query: 333  IPFGAGRRICLGMRLGLRMTTFLLASLVHGFDWALPDGLTPETLNMDVEFGLTLERAVPL 154
            IPFGAGRRIC GM LGLRM  F+ A+LVHGF+W LP+G+  E L+M+  +GLTL+R VPL
Sbjct: 428  IPFGAGRRICAGMSLGLRMVQFMTATLVHGFEWGLPEGVNAEKLDMEESYGLTLQRKVPL 487

Query: 153  VARPIPRLDPKAYVV 109
              +PIPRL   AY V
Sbjct: 488  TVQPIPRLVRGAYEV 502


>gb|AGC29948.1| CYP75B65 [Sinopodophyllum hexandrum]
          Length = 510

 Score =  294 bits (753), Expect = 2e-77
 Identities = 151/263 (57%), Positives = 185/263 (70%)
 Frame = -3

Query: 903  IEEHKTRPKIESEAEAEGKDFLSVLLGLKEADIKDEETDNKLTDIDIKALXXXXXXXXXX 724
            +EEH  R    S +  +  D LSVLLGLK+ D+  E    KLTD +IKAL          
Sbjct: 253  LEEH--RESENSNSGVKHTDLLSVLLGLKD-DVDGE--GGKLTDTNIKALLLDLFTAGTD 307

Query: 723  XXXXTVEWALVELIRHPDILAAAQKELDSIVGRSRLVSELDLNNVPVLQAIIKETFRLHP 544
                TVEWA+ EL+R+P +LA AQ+ELD +VGR RLVSE DL+ +   QAIIKETFRLHP
Sbjct: 308  TSSSTVEWAISELVRNPKLLAQAQEELDRVVGRDRLVSESDLSQLTFFQAIIKETFRLHP 367

Query: 543  PVPLLIPHTAAEACEVAGYHIPKAATLLVNTWTICRDPDVWSRPLEFDPSRFLPGAKHAD 364
              PL +P  A E+CE+ G++IPK +TLLVN W I RDP VW  PLEF P RF+PG ++A 
Sbjct: 368  STPLSLPRMATESCEINGFYIPKDSTLLVNVWAIARDPSVWPEPLEFKPERFVPGGRNAH 427

Query: 363  VDVKGSHFELIPFGAGRRICLGMRLGLRMTTFLLASLVHGFDWALPDGLTPETLNMDVEF 184
            +DVKG+ FE+IPFGAGRRIC GM +G+RM TF+ A+LVHGF+W LP+G  PE LNMD  +
Sbjct: 428  MDVKGNDFEVIPFGAGRRICAGMSMGIRMVTFVAATLVHGFNWELPEGQMPEKLNMDEAY 487

Query: 183  GLTLERAVPLVARPIPRLDPKAY 115
            GLTL+RAVPLV  P PRL P AY
Sbjct: 488  GLTLQRAVPLVVHPQPRLAPHAY 510