BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00002966
         (1707 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAT12274.1| obtusifoliol 14alpha-demethylase [Solanum chacoense]   808   0.0  
ref|NP_001234537.1| obtusifoliol 14alpha-demethylase [Solanum ly...   803   0.0  
gb|AAL54888.1|AF116915_1 obtusifoliol-14-demethylase [Nicotiana ...   801   0.0  
gb|ADN39418.1| obtusifoliol-14-demethylase [Withania somnifera]       801   0.0  
ref|XP_002271942.1| PREDICTED: sterol 14-demethylase [Vitis vini...   799   0.0  

>gb|AAT12274.1| obtusifoliol 14alpha-demethylase [Solanum chacoense]
          Length = 487

 Score =  808 bits (2086), Expect = 0.0
 Identities = 383/487 (78%), Positives = 442/487 (90%)
 Frame = +3

Query: 219  MDLKENKFFSIGLVFLATIIFIKLLAALFRSGSKHRQPPVVKSLPVIGGLLRFMKGPILL 398
            M+L +NK  ++GL+ +AT++  KL++AL    SK R PPV+K+LP++GGL+RF+KGPI++
Sbjct: 1    MELGDNKILNVGLLLVATLLVAKLISALIMPRSKKRLPPVIKALPIVGGLIRFLKGPIVM 60

Query: 399  IRQEYQKLGSVFTLNVVNRKITFFVGPEVSSHFFKAPEVELSQQEVYQFNVPTFGPGVVF 578
            +RQEY KLGSVFTLN++N+ ITFF+GPEVS+HFFKAPE +LSQQEVYQFNVPTFGPGVVF
Sbjct: 61   LRQEYPKLGSVFTLNLLNKNITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVF 120

Query: 579  DVDYSVRQEQFRFFTESLRVSKLKSYVDQMVAEAEDYFSKWGENGEVDLKYELEHLIILT 758
            DVDY++RQEQFRFFTE+LRV+KLK YVDQMV EAE+YFSKWGE+GEVDLKYELEHLIILT
Sbjct: 121  DVDYTIRQEQFRFFTEALRVTKLKGYVDQMVTEAEEYFSKWGESGEVDLKYELEHLIILT 180

Query: 759  ASRCLLGREVRDKLFDDVSALFHDLDNGMRPISVIFPYLPIPXXXXXXXXXXXXXEIFST 938
            ASRCLLG EVR+KLFDDVSALFHDLDNGM PISVIFPYLPIP             EIF+ 
Sbjct: 181  ASRCLLGEEVRNKLFDDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAEIFAN 240

Query: 939  IINSRKVSGKSEDDMLQCFIESKYKDGRPTTDGEITGLLIAALFAGQHTSSITSTWTGAY 1118
            IINSRK +GK+E+DMLQCFI+SKYKDGRPTT+GEITGLLIAALFAGQHTSSITSTWTG+Y
Sbjct: 241  IINSRKRTGKAENDMLQCFIDSKYKDGRPTTEGEITGLLIAALFAGQHTSSITSTWTGSY 300

Query: 1119 LLRFKQYLAAALDEQKQLMKKHGDKVNHDILAEMDVLYRCIKEALRLHPPLIMLLRQSHA 1298
            LL   +Y++A +DEQK LMKKHG+KV+HDIL+EMDVLYRCIKEALRLHPPLIMLLR SH+
Sbjct: 301  LLTNNKYMSAVVDEQKNLMKKHGNKVDHDILSEMDVLYRCIKEALRLHPPLIMLLRSSHS 360

Query: 1299 DFTVKTKEGMEYDIPKGHIVATSPAFANRLPYIYKDPDTYDPDRFAPGREEDKVAGAFSY 1478
            DF+V T+EG EYDIPKGHIVATSPAFANRLP+I+K+P++YDPDRF PGREEDK AGAFSY
Sbjct: 361  DFSVTTREGKEYDIPKGHIVATSPAFANRLPHIFKNPESYDPDRFGPGREEDKAAGAFSY 420

Query: 1479 ISFGGGRHGCLGEPFAYLQIKAIWTHLLRNFEFELVSPFPEIDWNAMVVGVKGQVMVRYK 1658
            ISFGGGRHGCLGEPFAYLQIKAIW+HLLRNFEFEL+SPFPEIDWNAMVVGVKG+VMV+YK
Sbjct: 421  ISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGEVMVKYK 480

Query: 1659 RRKLSVD 1679
            RRKLS +
Sbjct: 481  RRKLSAE 487


>ref|NP_001234537.1| obtusifoliol 14alpha-demethylase [Solanum lycopersicum]
            gi|299735181|gb|ADJ37071.1| obtusifoliol
            14alpha-demethylase [Solanum lycopersicum]
          Length = 487

 Score =  803 bits (2075), Expect = 0.0
 Identities = 382/487 (78%), Positives = 441/487 (90%)
 Frame = +3

Query: 219  MDLKENKFFSIGLVFLATIIFIKLLAALFRSGSKHRQPPVVKSLPVIGGLLRFMKGPILL 398
            M+L +NK  ++GL+ +AT++  KL++AL    SK R PPVVK+ P++GGL+RF+KGP+++
Sbjct: 1    MELGDNKILNVGLLLVATLLVAKLISALIMPRSKKRLPPVVKAWPIVGGLIRFLKGPVVM 60

Query: 399  IRQEYQKLGSVFTLNVVNRKITFFVGPEVSSHFFKAPEVELSQQEVYQFNVPTFGPGVVF 578
            +RQEY KLGSVFTLN++N+ ITFF+GPEVS+HFFKAPE +LSQQEVYQFNVPTFGPGVVF
Sbjct: 61   LRQEYPKLGSVFTLNLLNKNITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVF 120

Query: 579  DVDYSVRQEQFRFFTESLRVSKLKSYVDQMVAEAEDYFSKWGENGEVDLKYELEHLIILT 758
            DVDY++RQEQFRFFTE+LRV+KLK YVDQMV EAE+YFSKWGE+GEVDLKYELEHLIILT
Sbjct: 121  DVDYTIRQEQFRFFTEALRVTKLKGYVDQMVTEAEEYFSKWGESGEVDLKYELEHLIILT 180

Query: 759  ASRCLLGREVRDKLFDDVSALFHDLDNGMRPISVIFPYLPIPXXXXXXXXXXXXXEIFST 938
            ASRCLLG EVR+KLFDDVSALFHDLDNGM PISVIFPYLPIP             EIF+ 
Sbjct: 181  ASRCLLGEEVRNKLFDDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAEIFAN 240

Query: 939  IINSRKVSGKSEDDMLQCFIESKYKDGRPTTDGEITGLLIAALFAGQHTSSITSTWTGAY 1118
            IINSRK +GK+E+DMLQCFI+SKYKDGRPTT+GEITGLLIAALFAGQHTSSITSTWTG+Y
Sbjct: 241  IINSRKRTGKAENDMLQCFIDSKYKDGRPTTEGEITGLLIAALFAGQHTSSITSTWTGSY 300

Query: 1119 LLRFKQYLAAALDEQKQLMKKHGDKVNHDILAEMDVLYRCIKEALRLHPPLIMLLRQSHA 1298
            LL   +Y++A +DEQK LMKKHG+KV+HDIL+EM+VLYRCIKEALRLHPPLIMLLR SH+
Sbjct: 301  LLTNDKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHS 360

Query: 1299 DFTVKTKEGMEYDIPKGHIVATSPAFANRLPYIYKDPDTYDPDRFAPGREEDKVAGAFSY 1478
            +F+V T+EG EYDI KGHIVATSPAFANRLP+I+K+PDTYDPDRF PGREEDK AGAFSY
Sbjct: 361  EFSVTTREGKEYDISKGHIVATSPAFANRLPHIFKNPDTYDPDRFGPGREEDKAAGAFSY 420

Query: 1479 ISFGGGRHGCLGEPFAYLQIKAIWTHLLRNFEFELVSPFPEIDWNAMVVGVKGQVMVRYK 1658
            ISFGGGRHGCLGEPFAYLQIKAIW+HLLRNFEFEL+SPFPEIDWNAMVVGVKG+VMV+YK
Sbjct: 421  ISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGEVMVKYK 480

Query: 1659 RRKLSVD 1679
            RRKLSV+
Sbjct: 481  RRKLSVE 487


>gb|AAL54888.1|AF116915_1 obtusifoliol-14-demethylase [Nicotiana tabacum]
          Length = 487

 Score =  801 bits (2070), Expect = 0.0
 Identities = 383/487 (78%), Positives = 439/487 (90%)
 Frame = +3

Query: 219  MDLKENKFFSIGLVFLATIIFIKLLAALFRSGSKHRQPPVVKSLPVIGGLLRFMKGPILL 398
            M+L +NK  + GL+ +AT++  KL++AL    SK R PPV+KS P++GGLLRF+KGP+++
Sbjct: 1    MELGDNKILNAGLLLVATLVVAKLISALIMPRSKKRLPPVIKSWPILGGLLRFLKGPVVM 60

Query: 399  IRQEYQKLGSVFTLNVVNRKITFFVGPEVSSHFFKAPEVELSQQEVYQFNVPTFGPGVVF 578
            +R+EY KLGSVFTLN++N+ ITFFVGPEVS+HFFKAPE +LSQQEVYQFNVPTFGPGVVF
Sbjct: 61   LREEYPKLGSVFTLNLLNKNITFFVGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVF 120

Query: 579  DVDYSVRQEQFRFFTESLRVSKLKSYVDQMVAEAEDYFSKWGENGEVDLKYELEHLIILT 758
            DVDY++RQEQFRFFTESLRV+KLK YVDQMV EAE+YFSKWG++GEVDLKYELEHLIILT
Sbjct: 121  DVDYTIRQEQFRFFTESLRVNKLKGYVDQMVMEAEEYFSKWGDSGEVDLKYELEHLIILT 180

Query: 759  ASRCLLGREVRDKLFDDVSALFHDLDNGMRPISVIFPYLPIPXXXXXXXXXXXXXEIFST 938
            ASRCLLG EVR+KLF+DVSALFHDLDNGM PISVIFPYLPIP             EIF+ 
Sbjct: 181  ASRCLLGEEVRNKLFEDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAEIFAN 240

Query: 939  IINSRKVSGKSEDDMLQCFIESKYKDGRPTTDGEITGLLIAALFAGQHTSSITSTWTGAY 1118
            II+SRK +GK+E DMLQCFI+SKYKDGR TT+ EITGLLIAALFAGQHTSSITSTW GAY
Sbjct: 241  IIDSRKRTGKAESDMLQCFIDSKYKDGRATTESEITGLLIAALFAGQHTSSITSTWAGAY 300

Query: 1119 LLRFKQYLAAALDEQKQLMKKHGDKVNHDILAEMDVLYRCIKEALRLHPPLIMLLRQSHA 1298
            LL   +Y++A +DEQK LMKKHG+KV+HDIL+EM+VLYRCIKEALRLHPPLIMLLR SH+
Sbjct: 301  LLCNNKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHS 360

Query: 1299 DFTVKTKEGMEYDIPKGHIVATSPAFANRLPYIYKDPDTYDPDRFAPGREEDKVAGAFSY 1478
            DFTVKT+EG EYDIPKGHIVATSPAFANRLP++YK+PDTYDPDRFAPGR+EDKVAGAFSY
Sbjct: 361  DFTVKTREGKEYDIPKGHIVATSPAFANRLPHVYKNPDTYDPDRFAPGRDEDKVAGAFSY 420

Query: 1479 ISFGGGRHGCLGEPFAYLQIKAIWTHLLRNFEFELVSPFPEIDWNAMVVGVKGQVMVRYK 1658
            ISFGGGRHGCLGEPFAYLQIKAIW+HLLRNFEFEL+SPFPEIDWNAMVVGVKG+VMV+YK
Sbjct: 421  ISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGKVMVKYK 480

Query: 1659 RRKLSVD 1679
            RRKLS +
Sbjct: 481  RRKLSTE 487


>gb|ADN39418.1| obtusifoliol-14-demethylase [Withania somnifera]
          Length = 487

 Score =  801 bits (2068), Expect = 0.0
 Identities = 381/487 (78%), Positives = 439/487 (90%)
 Frame = +3

Query: 219  MDLKENKFFSIGLVFLATIIFIKLLAALFRSGSKHRQPPVVKSLPVIGGLLRFMKGPILL 398
            M+L +NKF ++GL+ +AT++  KL++AL    SK R PPV+ S P++GGL+RF+KGP+++
Sbjct: 1    MELSDNKFLNVGLLLVATLVVAKLMSALIMPRSKKRLPPVINSWPILGGLIRFLKGPVVM 60

Query: 399  IRQEYQKLGSVFTLNVVNRKITFFVGPEVSSHFFKAPEVELSQQEVYQFNVPTFGPGVVF 578
            +R+EY KLGSVFTLN++N+ ITFF+GPEVS+HFFKAPE +LSQQEVYQFNVPTFGPGVVF
Sbjct: 61   LREEYPKLGSVFTLNILNKNITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVF 120

Query: 579  DVDYSVRQEQFRFFTESLRVSKLKSYVDQMVAEAEDYFSKWGENGEVDLKYELEHLIILT 758
            DVDY++RQEQF FFTE+LRV+KLK YVDQMV EAE+YFSKWG++GEVDLKYELEHLIILT
Sbjct: 121  DVDYTIRQEQFTFFTEALRVTKLKGYVDQMVMEAEEYFSKWGDSGEVDLKYELEHLIILT 180

Query: 759  ASRCLLGREVRDKLFDDVSALFHDLDNGMRPISVIFPYLPIPXXXXXXXXXXXXXEIFST 938
            ASRCLLG+EV +KLF DVSALFHDLDNGM PISVIFPYLPIP             EIF+ 
Sbjct: 181  ASRCLLGQEVPNKLFADVSALFHDLDNGMLPISVIFPYLPIPAHRRRDKARKKLAEIFAN 240

Query: 939  IINSRKVSGKSEDDMLQCFIESKYKDGRPTTDGEITGLLIAALFAGQHTSSITSTWTGAY 1118
            IINSRK +GK+E+DMLQCFI+SKYKDGR TT+GEITGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241  IINSRKRTGKAENDMLQCFIDSKYKDGRATTEGEITGLLIAALFAGQHTSSITSTWTGAY 300

Query: 1119 LLRFKQYLAAALDEQKQLMKKHGDKVNHDILAEMDVLYRCIKEALRLHPPLIMLLRQSHA 1298
            LL  K+Y++A +DEQK LMKKHG+KV+HDIL+EM+VLYRCIKEALRLHPPLIMLLR SH+
Sbjct: 301  LLCNKKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRHSHS 360

Query: 1299 DFTVKTKEGMEYDIPKGHIVATSPAFANRLPYIYKDPDTYDPDRFAPGREEDKVAGAFSY 1478
            DF V TKEG EYDIPKGHIVATSPAFANRLP+IYK+PDTYDPDRF+PGREEDK AGAFSY
Sbjct: 361  DFCVTTKEGKEYDIPKGHIVATSPAFANRLPHIYKNPDTYDPDRFSPGREEDKAAGAFSY 420

Query: 1479 ISFGGGRHGCLGEPFAYLQIKAIWTHLLRNFEFELVSPFPEIDWNAMVVGVKGQVMVRYK 1658
            ISFGGGRHGCLGEPFAYLQIKAIW+HLLRNFEFEL+SPFPEIDWNAMVVGVKG+VMV+YK
Sbjct: 421  ISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGEVMVKYK 480

Query: 1659 RRKLSVD 1679
            RRKLS +
Sbjct: 481  RRKLSTE 487


>ref|XP_002271942.1| PREDICTED: sterol 14-demethylase [Vitis vinifera]
          Length = 486

 Score =  799 bits (2063), Expect = 0.0
 Identities = 384/483 (79%), Positives = 433/483 (89%)
 Frame = +3

Query: 231  ENKFFSIGLVFLATIIFIKLLAALFRSGSKHRQPPVVKSLPVIGGLLRFMKGPILLIRQE 410
            +NKFF++ L+ +AT++  KL++AL    S+ R PP VK+ PVIGGLLRF+KGP++++R+E
Sbjct: 4    DNKFFNVALLIVATVVVAKLISALLIPKSRKRLPPTVKAFPVIGGLLRFLKGPVVMLREE 63

Query: 411  YQKLGSVFTLNVVNRKITFFVGPEVSSHFFKAPEVELSQQEVYQFNVPTFGPGVVFDVDY 590
            Y KLGSVFTLN++N+ ITFF+GPEVS+HFFKAPE +LSQQEVYQFNVPTFGPGVVFDVDY
Sbjct: 64   YPKLGSVFTLNLLNKNITFFIGPEVSAHFFKAPEADLSQQEVYQFNVPTFGPGVVFDVDY 123

Query: 591  SVRQEQFRFFTESLRVSKLKSYVDQMVAEAEDYFSKWGENGEVDLKYELEHLIILTASRC 770
            SVRQEQFRFFTESLRV+KLK YVDQMV E EDYFSKWG++GEVDLKYELEHLIILTASRC
Sbjct: 124  SVRQEQFRFFTESLRVTKLKGYVDQMVTETEDYFSKWGDSGEVDLKYELEHLIILTASRC 183

Query: 771  LLGREVRDKLFDDVSALFHDLDNGMRPISVIFPYLPIPXXXXXXXXXXXXXEIFSTIINS 950
            LLG+EVRDKLF DVSALFHDLDNGM PISVIFPYLPIP              IF+ II S
Sbjct: 184  LLGQEVRDKLFADVSALFHDLDNGMLPISVIFPYLPIPAHRRRDQARTKLAHIFANIIAS 243

Query: 951  RKVSGKSEDDMLQCFIESKYKDGRPTTDGEITGLLIAALFAGQHTSSITSTWTGAYLLRF 1130
            R+ +GKSE+DMLQCF++SKYKDGR TT+ E+TGLLIAALFAGQHTSSITSTWTGAYL R 
Sbjct: 244  RRETGKSENDMLQCFMDSKYKDGRQTTEAEVTGLLIAALFAGQHTSSITSTWTGAYLFRH 303

Query: 1131 KQYLAAALDEQKQLMKKHGDKVNHDILAEMDVLYRCIKEALRLHPPLIMLLRQSHADFTV 1310
            K++L+A LDEQK LMKKHG+KV+HDIL+EMDVLYRCIKEALRLHPPLIMLLR SH+DF+V
Sbjct: 304  KEFLSAVLDEQKNLMKKHGNKVDHDILSEMDVLYRCIKEALRLHPPLIMLLRSSHSDFSV 363

Query: 1311 KTKEGMEYDIPKGHIVATSPAFANRLPYIYKDPDTYDPDRFAPGREEDKVAGAFSYISFG 1490
             TK+G EYDIPKGHIVATSPAFANRLP+IYKDP+ YDPDRFA GREEDKVAGAFSYISFG
Sbjct: 364  TTKDGKEYDIPKGHIVATSPAFANRLPHIYKDPERYDPDRFAVGREEDKVAGAFSYISFG 423

Query: 1491 GGRHGCLGEPFAYLQIKAIWTHLLRNFEFELVSPFPEIDWNAMVVGVKGQVMVRYKRRKL 1670
            GGRHGCLGEPFAYLQIKAIW+HLLRNFEFEL+SPFPEIDWNAMVVGVKG+VMVRYKRR L
Sbjct: 424  GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGKVMVRYKRRVL 483

Query: 1671 SVD 1679
             VD
Sbjct: 484  PVD 486