BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00002792
         (1786 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast prec...   849   0.0  
sp|Q1W2L8.2|GSH1_TOBAC RecName: Full=Glutamate--cysteine ligase,...   844   0.0  
gb|AFF18844.2| gamma-glutamylcysteine synthetase [Dimocarpus lon...   842   0.0  
gb|AAL61610.1|AF401621_1 gamma-glutamylcysteine synthetase [Alli...   837   0.0  
gb|ACQ91100.1| GSH1 [Solanum lycopersicum]                            835   0.0  

>ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast precursor, putative [Ricinus
            communis] gi|223549699|gb|EEF51187.1| Glutamate--cysteine
            ligase, chloroplast precursor, putative [Ricinus
            communis]
          Length = 526

 Score =  849 bits (2193), Expect = 0.0
 Identities = 403/449 (89%), Positives = 427/449 (95%)
 Frame = -1

Query: 1561 AASPPTEDAAVMTEPLTKEDLVGYLASGCKAKEKWRIGTEHEKFGFEVGTLRPIKYEQIA 1382
            AASPP EDA + TEPLTKEDLVGYLASGCK KEKWRIGTEHEKFGFE+GTLRP+KYEQIA
Sbjct: 78   AASPPIEDAVIATEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFELGTLRPMKYEQIA 137

Query: 1381 ELLNGLAERFDWDKIIENDYVIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHL 1202
            ELLNG+AERFDW+K++E DY+IGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHL
Sbjct: 138  ELLNGIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHL 197

Query: 1201 YQVKAVAEDMGIGFLGIGFQPKWALKDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTV 1022
            YQVKAV E+MGIGFLGIGFQPKW +KDIPIMPKGRYEIMRNYMPKVG+LGLDMMFRTCTV
Sbjct: 198  YQVKAVTEEMGIGFLGIGFQPKWGVKDIPIMPKGRYEIMRNYMPKVGTLGLDMMFRTCTV 257

Query: 1021 QVNLDFSSEADMIRKFRAGLALQPIATAIFANSPFTEGKPNGYLSMRSNIWTDTDNNRAG 842
            QVNLDFSSEADMIRKFRAGLALQPIATA+FANSPFTEGKPNGYLSMRS IWTDTD +R G
Sbjct: 258  QVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSQIWTDTDKDRTG 317

Query: 841  MLPFVFDDSFGFERYVDYALDVPMYFVYRNKKYIDCTGMSFRDFMAGKLAPVPGELPTLN 662
            MLPFVFDDSFGFE+YVDYALDVPMYFVYR KKYIDCTGM+FRDF+AGKL  +PGELPTLN
Sbjct: 318  MLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLPCIPGELPTLN 377

Query: 661  DWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEVSLQNILDLTADWT 482
            DWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDE+SLQN+LD+TADWT
Sbjct: 378  DWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEISLQNVLDMTADWT 437

Query: 481  NEEREMLRRKVPVTGLKTPFRDGLLRHVAEDVLQFAKDGLERRGYKEAGFLKEVTSVVST 302
             EEREMLR KVP TGLKTPFRDGLL+HVAEDVL+ AKDGLERRGYKE GFL EVT VV T
Sbjct: 438  PEEREMLRNKVPKTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEIGFLNEVTEVVRT 497

Query: 301  GVTPAENLLELYHGKWGQNVDPVFEELLY 215
            GVTPAE LLELY+GKWGQ+VDPVFEELLY
Sbjct: 498  GVTPAERLLELYNGKWGQSVDPVFEELLY 526


>sp|Q1W2L8.2|GSH1_TOBAC RecName: Full=Glutamate--cysteine ligase, chloroplastic; AltName:
            Full=Gamma-ECS; Short=GCS; AltName:
            Full=Gamma-glutamylcysteine synthetase; Flags: Precursor
            gi|111380512|gb|ABD98695.2| chloroplast
            gamma-glutamylcysteine synthetase [Nicotiana tabacum]
          Length = 522

 Score =  844 bits (2181), Expect = 0.0
 Identities = 399/449 (88%), Positives = 426/449 (94%)
 Frame = -1

Query: 1561 AASPPTEDAAVMTEPLTKEDLVGYLASGCKAKEKWRIGTEHEKFGFEVGTLRPIKYEQIA 1382
            AASPPTEDA V  EPLTKEDLV YLASGCK+KEKWRIGTEHEKFGFE GTLRP+KYEQIA
Sbjct: 74   AASPPTEDAVVAAEPLTKEDLVAYLASGCKSKEKWRIGTEHEKFGFEFGTLRPMKYEQIA 133

Query: 1381 ELLNGLAERFDWDKIIENDYVIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHL 1202
            ELLNG+AERFDW+K++E D +IGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHL
Sbjct: 134  ELLNGIAERFDWEKVMEGDNIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHL 193

Query: 1201 YQVKAVAEDMGIGFLGIGFQPKWALKDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTV 1022
            YQVKAVAE+MGIGFLG GFQPKW LKDIP+MPKGRYEIMRNYMPKVGSLGLDMMFRTCTV
Sbjct: 194  YQVKAVAEEMGIGFLGTGFQPKWGLKDIPVMPKGRYEIMRNYMPKVGSLGLDMMFRTCTV 253

Query: 1021 QVNLDFSSEADMIRKFRAGLALQPIATAIFANSPFTEGKPNGYLSMRSNIWTDTDNNRAG 842
            QVNLDFSSEADMIRKFRAGLALQPIATA+FANSPFTEGKPNGYLSMRS+IWTDTDNNRAG
Sbjct: 254  QVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSHIWTDTDNNRAG 313

Query: 841  MLPFVFDDSFGFERYVDYALDVPMYFVYRNKKYIDCTGMSFRDFMAGKLAPVPGELPTLN 662
            MLPFVFDDSFGFE+YVDYALDVPMYFVYR KKYIDC GMSFRDFM GKL+P+PG+ PTLN
Sbjct: 314  MLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDFMNGKLSPIPGDYPTLN 373

Query: 661  DWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEVSLQNILDLTADWT 482
            DWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG+LYDEVSLQ +LD+T+DWT
Sbjct: 374  DWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQTVLDMTSDWT 433

Query: 481  NEEREMLRRKVPVTGLKTPFRDGLLRHVAEDVLQFAKDGLERRGYKEAGFLKEVTSVVST 302
             EEREMLR KVP +GLKTPFRDGLL+HVA+DV++ AK+GLERRGYKE GFL EVT VV T
Sbjct: 434  AEEREMLRNKVPTSGLKTPFRDGLLKHVAQDVVKLAKEGLERRGYKETGFLNEVTEVVRT 493

Query: 301  GVTPAENLLELYHGKWGQNVDPVFEELLY 215
            GVTPAE LLELYHGKWG++VDPVFEELLY
Sbjct: 494  GVTPAEKLLELYHGKWGRSVDPVFEELLY 522


>gb|AFF18844.2| gamma-glutamylcysteine synthetase [Dimocarpus longan]
          Length = 511

 Score =  842 bits (2174), Expect = 0.0
 Identities = 398/449 (88%), Positives = 426/449 (94%)
 Frame = -1

Query: 1561 AASPPTEDAAVMTEPLTKEDLVGYLASGCKAKEKWRIGTEHEKFGFEVGTLRPIKYEQIA 1382
            AASPPT+DA +  EPLTKEDLVGYLASGCK +EKWRIGTEHEKFGFE+GTLRP+KYEQIA
Sbjct: 63   AASPPTDDAVIAAEPLTKEDLVGYLASGCKPREKWRIGTEHEKFGFEIGTLRPMKYEQIA 122

Query: 1381 ELLNGLAERFDWDKIIENDYVIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHL 1202
            ELLN +AERFDW+KI+E++ +IGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHL
Sbjct: 123  ELLNSIAERFDWEKIMEDNNIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHL 182

Query: 1201 YQVKAVAEDMGIGFLGIGFQPKWALKDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTV 1022
            YQVKAVAE+MGIGFLGIGFQPKW +KDIP+MPKGRYEIM+NYMPKVGSLGLDMMFRTCTV
Sbjct: 183  YQVKAVAEEMGIGFLGIGFQPKWGIKDIPVMPKGRYEIMKNYMPKVGSLGLDMMFRTCTV 242

Query: 1021 QVNLDFSSEADMIRKFRAGLALQPIATAIFANSPFTEGKPNGYLSMRSNIWTDTDNNRAG 842
            QVNLDFSSEADMIRKFRAGLALQPIATA+FANSPFTEGKPNGYLSMRS IWTDTDNNR G
Sbjct: 243  QVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSQIWTDTDNNRTG 302

Query: 841  MLPFVFDDSFGFERYVDYALDVPMYFVYRNKKYIDCTGMSFRDFMAGKLAPVPGELPTLN 662
            MLPFVFDDSFGFE+YVDYALDVPMYFVYR KKYIDCTGMSFRDF+ GKL  +PGELPTLN
Sbjct: 303  MLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMSFRDFLVGKLPCLPGELPTLN 362

Query: 661  DWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEVSLQNILDLTADWT 482
            DWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEVSLQN LDLTADWT
Sbjct: 363  DWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEVSLQNALDLTADWT 422

Query: 481  NEEREMLRRKVPVTGLKTPFRDGLLRHVAEDVLQFAKDGLERRGYKEAGFLKEVTSVVST 302
            +EER+MLR KVP TGLKTPFRDGLLRHVA+D+L+ AKDGLERRG+KE+GFL  V  VV T
Sbjct: 423  SEERQMLRNKVPKTGLKTPFRDGLLRHVAQDILKLAKDGLERRGFKESGFLNAVAEVVRT 482

Query: 301  GVTPAENLLELYHGKWGQNVDPVFEELLY 215
            GVTPAE LLE+YHGKWGQ+VDPVFEELLY
Sbjct: 483  GVTPAEKLLEMYHGKWGQSVDPVFEELLY 511


>gb|AAL61610.1|AF401621_1 gamma-glutamylcysteine synthetase [Allium cepa]
          Length = 496

 Score =  837 bits (2162), Expect = 0.0
 Identities = 391/449 (87%), Positives = 429/449 (95%)
 Frame = -1

Query: 1561 AASPPTEDAAVMTEPLTKEDLVGYLASGCKAKEKWRIGTEHEKFGFEVGTLRPIKYEQIA 1382
            +ASPPTEDA VMTEPLTK+DLV YLASGCK K+KWRIGTEHEKFGF++ TLRP+ YEQIA
Sbjct: 48   SASPPTEDAVVMTEPLTKDDLVAYLASGCKTKDKWRIGTEHEKFGFQINTLRPMTYEQIA 107

Query: 1381 ELLNGLAERFDWDKIIENDYVIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHL 1202
             LLNG+AERFDW+K++E DYVIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHL
Sbjct: 108  YLLNGIAERFDWEKVMEGDYVIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHL 167

Query: 1201 YQVKAVAEDMGIGFLGIGFQPKWALKDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTV 1022
            YQVKAVAE+MGIGFLG GFQPKW LKDIPIMPKGRYEIMRNYMPKVGS+GLDMMFRTCTV
Sbjct: 168  YQVKAVAEEMGIGFLGAGFQPKWGLKDIPIMPKGRYEIMRNYMPKVGSMGLDMMFRTCTV 227

Query: 1021 QVNLDFSSEADMIRKFRAGLALQPIATAIFANSPFTEGKPNGYLSMRSNIWTDTDNNRAG 842
            QVNLDFSSE+DM+RKFRAGL+LQPIATAIFANSPFTEGKPNG+LS RS+IWTDTDNNR+G
Sbjct: 228  QVNLDFSSESDMVRKFRAGLSLQPIATAIFANSPFTEGKPNGFLSKRSHIWTDTDNNRSG 287

Query: 841  MLPFVFDDSFGFERYVDYALDVPMYFVYRNKKYIDCTGMSFRDFMAGKLAPVPGELPTLN 662
            MLPFVF DSFGFE+YVDYALDVPMYFVYRNKKYIDC+G+SFRDFM GKL+PVPGELPTLN
Sbjct: 288  MLPFVFKDSFGFEQYVDYALDVPMYFVYRNKKYIDCSGLSFRDFMKGKLSPVPGELPTLN 347

Query: 661  DWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEVSLQNILDLTADWT 482
            DWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYD+++LQ+++++TADWT
Sbjct: 348  DWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDDIALQSVIEITADWT 407

Query: 481  NEEREMLRRKVPVTGLKTPFRDGLLRHVAEDVLQFAKDGLERRGYKEAGFLKEVTSVVST 302
             EER+MLRRKVPVTGLKTPFRDGLL+HVAEDVL+ A+ GLERRGYKE GFL+EV  VV+T
Sbjct: 408  REERDMLRRKVPVTGLKTPFRDGLLKHVAEDVLKLAQGGLERRGYKEVGFLREVAEVVNT 467

Query: 301  GVTPAENLLELYHGKWGQNVDPVFEELLY 215
            GVTPAE LLELYHGKWG NVDP+F+ELLY
Sbjct: 468  GVTPAEKLLELYHGKWGCNVDPIFQELLY 496


>gb|ACQ91100.1| GSH1 [Solanum lycopersicum]
          Length = 523

 Score =  835 bits (2156), Expect = 0.0
 Identities = 394/449 (87%), Positives = 423/449 (94%)
 Frame = -1

Query: 1561 AASPPTEDAAVMTEPLTKEDLVGYLASGCKAKEKWRIGTEHEKFGFEVGTLRPIKYEQIA 1382
            AASPPTEDA V  EPLTKEDLVGYLASGCK+KEKWRIGTEHEKFGFE GTLRP+KY+QIA
Sbjct: 75   AASPPTEDAVVAAEPLTKEDLVGYLASGCKSKEKWRIGTEHEKFGFEFGTLRPMKYDQIA 134

Query: 1381 ELLNGLAERFDWDKIIENDYVIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHL 1202
            +LLNG+AERFDW+K++E D +IGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHL
Sbjct: 135  DLLNGIAERFDWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGAPLETLHQTCAEVNSHL 194

Query: 1201 YQVKAVAEDMGIGFLGIGFQPKWALKDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTV 1022
            YQVKAVAE+MGIGFLG GFQPKW LKDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTV
Sbjct: 195  YQVKAVAEEMGIGFLGTGFQPKWGLKDIPIMPKGRYEIMRNYMPKVGSLGLDMMFRTCTV 254

Query: 1021 QVNLDFSSEADMIRKFRAGLALQPIATAIFANSPFTEGKPNGYLSMRSNIWTDTDNNRAG 842
            QVNLDFSSEADMIRKFRAGLALQPIATA+FANSPFTEGKPNGYLS RS+IWTDTDNNRAG
Sbjct: 255  QVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSKRSHIWTDTDNNRAG 314

Query: 841  MLPFVFDDSFGFERYVDYALDVPMYFVYRNKKYIDCTGMSFRDFMAGKLAPVPGELPTLN 662
            MLPFVFDDSFGFE+YVDYALDVPMYFVYR KKY+DCTG+SFRDFM GKL P+PGE PTLN
Sbjct: 315  MLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYVDCTGLSFRDFMNGKLPPIPGEYPTLN 374

Query: 661  DWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEVSLQNILDLTADWT 482
            DWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG+LYDE SLQ++LD+T DWT
Sbjct: 375  DWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEGSLQSVLDMTFDWT 434

Query: 481  NEEREMLRRKVPVTGLKTPFRDGLLRHVAEDVLQFAKDGLERRGYKEAGFLKEVTSVVST 302
             EER+MLR KVP +GLKTPFRDGLL HVA+DV++ AK+GLERRG+KE GFL EV  VV T
Sbjct: 435  AEERDMLRNKVPKSGLKTPFRDGLLMHVAQDVVKLAKEGLERRGFKETGFLNEVAEVVKT 494

Query: 301  GVTPAENLLELYHGKWGQNVDPVFEELLY 215
            GVTPAE LLELYHGKWGQ+VDP+FEELLY
Sbjct: 495  GVTPAEKLLELYHGKWGQSVDPIFEELLY 523