BLASTX nr result
ID: Dioscorea21_contig00001352
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dioscorea21_contig00001352
(2344 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|P49299.1|CYSZ_CUCMA RecName: Full=Citrate synthase, glyoxysom... 472 e-130
ref|XP_004167690.1| PREDICTED: citrate synthase, glyoxysomal-lik... 470 e-130
ref|XP_004143228.1| PREDICTED: citrate synthase, glyoxysomal-lik... 470 e-130
ref|XP_002322875.1| predicted protein [Populus trichocarpa] gi|2... 468 e-129
dbj|BAJ34181.1| unnamed protein product [Thellungiella halophila] 466 e-128
>sp|P49299.1|CYSZ_CUCMA RecName: Full=Citrate synthase, glyoxysomal; AltName: Full=GCS;
Flags: Precursor gi|1084323|pir||S53007 citrate synthase
- cucurbit gi|975633|dbj|BAA07328.1| glyoxysomal citrate
synthase [Cucurbita cv. Kurokawa Amakuri]
Length = 516
Score = 472 bits (1215), Expect = e-130
Identities = 235/247 (95%), Positives = 243/247 (98%)
Frame = -3
Query: 1739 LRYRGYPIEELAESSTFTEVAYLLMYGNLPSQNQLADWEFAISQHSAVPQGLLDIIQAMP 1560
LRYRGYPIEELAESST+ EVAYLLMYGNLPSQ+QLADWEFAISQHSAVPQGL+DIIQAMP
Sbjct: 124 LRYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFAISQHSAVPQGLVDIIQAMP 183
Query: 1559 HDAHPMGVLVSAMSALSVFHPDANPALRGQDLYKLKQVRDKQIARILGKAPTIAAAAYLR 1380
HDAHPMGVLVSAMSALSVFHPDANPALRGQDLYK KQVRDKQIARI+GKAPTIAAAAYLR
Sbjct: 184 HDAHPMGVLVSAMSALSVFHPDANPALRGQDLYKSKQVRDKQIARIIGKAPTIAAAAYLR 243
Query: 1379 LAGRPPVLPSNNLSYSENFLYMLDSLGNHAYKPNPRLARVLDILFILHAEHEMNCSTAAA 1200
LAGRPPVLPS+NLSYSENFLYMLDSLGN +YKPNPRLARVLDILFILHAEHEMNCST+AA
Sbjct: 244 LAGRPPVLPSSNLSYSENFLYMLDSLGNRSYKPNPRLARVLDILFILHAEHEMNCSTSAA 303
Query: 1199 RHLASSGVDVFTALSGAVGALYGPLHGGANEAVLKMLNEIGTVENIPEFIEGVKNRKRKM 1020
RHLASSGVDVFTALSGAVGALYGPLHGGANEAVLKML+EIGTV NIPEFIEGVKNRKRKM
Sbjct: 304 RHLASSGVDVFTALSGAVGALYGPLHGGANEAVLKMLSEIGTVNNIPEFIEGVKNRKRKM 363
Query: 1019 SGFGHRV 999
SGFGHRV
Sbjct: 364 SGFGHRV 370
Score = 217 bits (552), Expect = 1e-53
Identities = 104/120 (86%), Positives = 112/120 (93%)
Frame = -2
Query: 864 DPLIEVAVALEKAALTDEYFIKRKLYPNVDFYSGLIYRAMGFPTEFFPVLFAIPRMAGYL 685
DPLIEVAVALEKAAL+DEYF+KRKLYPNVDFYSGLIYRAMGFP EFF VLFAIPRMAGYL
Sbjct: 395 DPLIEVAVALEKAALSDEYFVKRKLYPNVDFYSGLIYRAMGFPPEFFTVLFAIPRMAGYL 454
Query: 684 AHWRESLDDPDTKIMRPQQVYTGVWLRHYMPFRERTMPTEADSLGQVSVSNASRRRLAGS 505
AHWRESLDDPDTKI+RPQQVYTG WLRHY+P ER +P +AD LGQVSVSNAS+RRL+GS
Sbjct: 455 AHWRESLDDPDTKIIRPQQVYTGEWLRHYIPPNERLVPAKADRLGQVSVSNASKRRLSGS 514
Score = 99.8 bits (247), Expect = 3e-18
Identities = 49/96 (51%), Positives = 68/96 (70%), Gaps = 4/96 (4%)
Frame = -2
Query: 2139 EARFDPADLARGRLAVIAAHLATTSISPAL----LETSPVSAQVAARAPSALGGVLTVID 1972
+ P+++AR RLAV+AAHL+ S+ P + LE VSAQ P + G LT++D
Sbjct: 4 DMELSPSNVARHRLAVLAAHLSAASLEPPVMASSLEAHCVSAQTMVAPPELVKGTLTIVD 63
Query: 1971 ERTGKKYEIKVSDEGTVKASDFKKISTGNQDKGLKL 1864
ERTGK+Y+++VS+EGT+KA+D KKI+TG DKGLKL
Sbjct: 64 ERTGKRYQVQVSEEGTIKATDLKKITTGPNDKGLKL 99
>ref|XP_004167690.1| PREDICTED: citrate synthase, glyoxysomal-like [Cucumis sativus]
Length = 512
Score = 470 bits (1209), Expect = e-130
Identities = 233/247 (94%), Positives = 243/247 (98%)
Frame = -3
Query: 1739 LRYRGYPIEELAESSTFTEVAYLLMYGNLPSQNQLADWEFAISQHSAVPQGLLDIIQAMP 1560
LRYRGYPIEELAESST+ EVAYLLMYGNLPSQ+QLADWEFA+SQHSAVPQGLLDIIQAMP
Sbjct: 120 LRYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMP 179
Query: 1559 HDAHPMGVLVSAMSALSVFHPDANPALRGQDLYKLKQVRDKQIARILGKAPTIAAAAYLR 1380
HDAHPMGVLVSAMSALSVFHPDANPALRGQDLYK KQVRDKQIARI+GKAPTIAAAAYLR
Sbjct: 180 HDAHPMGVLVSAMSALSVFHPDANPALRGQDLYKSKQVRDKQIARIIGKAPTIAAAAYLR 239
Query: 1379 LAGRPPVLPSNNLSYSENFLYMLDSLGNHAYKPNPRLARVLDILFILHAEHEMNCSTAAA 1200
LAGRPPVLPS+NLSYSENFLYMLDSLGN +YKPNPRLARV+DILFILHAEHEMNCST+AA
Sbjct: 240 LAGRPPVLPSSNLSYSENFLYMLDSLGNRSYKPNPRLARVIDILFILHAEHEMNCSTSAA 299
Query: 1199 RHLASSGVDVFTALSGAVGALYGPLHGGANEAVLKMLNEIGTVENIPEFIEGVKNRKRKM 1020
RHLASSGVDVFTALSGAVGALYGPLHGGANEAVLKML+EIGTV+NIP FIEGVKNRKRKM
Sbjct: 300 RHLASSGVDVFTALSGAVGALYGPLHGGANEAVLKMLSEIGTVDNIPGFIEGVKNRKRKM 359
Query: 1019 SGFGHRV 999
SGFGHRV
Sbjct: 360 SGFGHRV 366
Score = 218 bits (554), Expect = 8e-54
Identities = 104/120 (86%), Positives = 113/120 (94%)
Frame = -2
Query: 864 DPLIEVAVALEKAALTDEYFIKRKLYPNVDFYSGLIYRAMGFPTEFFPVLFAIPRMAGYL 685
DPLIEVAVALEKAAL+DEYF+KRKLYPNVDFYSGLIYRAMGFP EFF VLFAIPRMAGYL
Sbjct: 391 DPLIEVAVALEKAALSDEYFVKRKLYPNVDFYSGLIYRAMGFPPEFFTVLFAIPRMAGYL 450
Query: 684 AHWRESLDDPDTKIMRPQQVYTGVWLRHYMPFRERTMPTEADSLGQVSVSNASRRRLAGS 505
AHWRESLDDPDTKI+RPQQVYTG WLRHY+P +ER +P +AD LGQVSVSNAS+RRL+GS
Sbjct: 451 AHWRESLDDPDTKIIRPQQVYTGEWLRHYIPPKERLVPAKADRLGQVSVSNASKRRLSGS 510
Score = 101 bits (252), Expect = 8e-19
Identities = 51/91 (56%), Positives = 72/91 (79%), Gaps = 4/91 (4%)
Frame = -2
Query: 2124 PADLARGRLAVIAAHLATTSI-SP---ALLETSPVSAQVAARAPSALGGVLTVIDERTGK 1957
P+++ARGRLAV+AAHL+ ++ SP +LLE + VSA+ P AL G LT++DERTGK
Sbjct: 5 PSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVDERTGK 64
Query: 1956 KYEIKVSDEGTVKASDFKKISTGNQDKGLKL 1864
+Y++++S+EGT+KA+D KKI+TG DKGLKL
Sbjct: 65 RYQVQISEEGTIKATDLKKITTGPNDKGLKL 95
>ref|XP_004143228.1| PREDICTED: citrate synthase, glyoxysomal-like [Cucumis sativus]
Length = 612
Score = 470 bits (1209), Expect = e-130
Identities = 233/247 (94%), Positives = 243/247 (98%)
Frame = -3
Query: 1739 LRYRGYPIEELAESSTFTEVAYLLMYGNLPSQNQLADWEFAISQHSAVPQGLLDIIQAMP 1560
LRYRGYPIEELAESST+ EVAYLLMYGNLPSQ+QLADWEFA+SQHSAVPQGLLDIIQAMP
Sbjct: 220 LRYRGYPIEELAESSTYVEVAYLLMYGNLPSQSQLADWEFALSQHSAVPQGLLDIIQAMP 279
Query: 1559 HDAHPMGVLVSAMSALSVFHPDANPALRGQDLYKLKQVRDKQIARILGKAPTIAAAAYLR 1380
HDAHPMGVLVSAMSALSVFHPDANPALRGQDLYK KQVRDKQIARI+GKAPTIAAAAYLR
Sbjct: 280 HDAHPMGVLVSAMSALSVFHPDANPALRGQDLYKSKQVRDKQIARIIGKAPTIAAAAYLR 339
Query: 1379 LAGRPPVLPSNNLSYSENFLYMLDSLGNHAYKPNPRLARVLDILFILHAEHEMNCSTAAA 1200
LAGRPPVLPS+NLSYSENFLYMLDSLGN +YKPNPRLARV+DILFILHAEHEMNCST+AA
Sbjct: 340 LAGRPPVLPSSNLSYSENFLYMLDSLGNRSYKPNPRLARVIDILFILHAEHEMNCSTSAA 399
Query: 1199 RHLASSGVDVFTALSGAVGALYGPLHGGANEAVLKMLNEIGTVENIPEFIEGVKNRKRKM 1020
RHLASSGVDVFTALSGAVGALYGPLHGGANEAVLKML+EIGTV+NIP FIEGVKNRKRKM
Sbjct: 400 RHLASSGVDVFTALSGAVGALYGPLHGGANEAVLKMLSEIGTVDNIPGFIEGVKNRKRKM 459
Query: 1019 SGFGHRV 999
SGFGHRV
Sbjct: 460 SGFGHRV 466
Score = 218 bits (554), Expect = 8e-54
Identities = 104/120 (86%), Positives = 113/120 (94%)
Frame = -2
Query: 864 DPLIEVAVALEKAALTDEYFIKRKLYPNVDFYSGLIYRAMGFPTEFFPVLFAIPRMAGYL 685
DPLIEVAVALEKAAL+DEYF+KRKLYPNVDFYSGLIYRAMGFP EFF VLFAIPRMAGYL
Sbjct: 491 DPLIEVAVALEKAALSDEYFVKRKLYPNVDFYSGLIYRAMGFPPEFFTVLFAIPRMAGYL 550
Query: 684 AHWRESLDDPDTKIMRPQQVYTGVWLRHYMPFRERTMPTEADSLGQVSVSNASRRRLAGS 505
AHWRESLDDPDTKI+RPQQVYTG WLRHY+P +ER +P +AD LGQVSVSNAS+RRL+GS
Sbjct: 551 AHWRESLDDPDTKIIRPQQVYTGEWLRHYIPPKERLVPAKADRLGQVSVSNASKRRLSGS 610
Score = 102 bits (253), Expect = 6e-19
Identities = 51/96 (53%), Positives = 73/96 (76%), Gaps = 4/96 (4%)
Frame = -2
Query: 2139 EARFDPADLARGRLAVIAAHLATTSI-SP---ALLETSPVSAQVAARAPSALGGVLTVID 1972
+ P+++ARGRLAV+AAHL+ ++ SP +LLE + VSA+ P AL G LT++D
Sbjct: 100 DMELSPSNVARGRLAVLAAHLSAATLESPVMASLLEANCVSARTMLPPPEALKGTLTIVD 159
Query: 1971 ERTGKKYEIKVSDEGTVKASDFKKISTGNQDKGLKL 1864
ERTGK+Y++++S+EGT+KA+D KKI+TG DKGLKL
Sbjct: 160 ERTGKRYQVQISEEGTIKATDLKKITTGPNDKGLKL 195
>ref|XP_002322875.1| predicted protein [Populus trichocarpa] gi|222867505|gb|EEF04636.1|
predicted protein [Populus trichocarpa]
Length = 508
Score = 468 bits (1205), Expect = e-129
Identities = 230/247 (93%), Positives = 244/247 (98%)
Frame = -3
Query: 1739 LRYRGYPIEELAESSTFTEVAYLLMYGNLPSQNQLADWEFAISQHSAVPQGLLDIIQAMP 1560
LRYRGYPIEELAESSTF EVAYL++YGNLPSQ+QLADWEFAISQHSAVPQG+LDIIQAMP
Sbjct: 116 LRYRGYPIEELAESSTFLEVAYLILYGNLPSQSQLADWEFAISQHSAVPQGVLDIIQAMP 175
Query: 1559 HDAHPMGVLVSAMSALSVFHPDANPALRGQDLYKLKQVRDKQIARILGKAPTIAAAAYLR 1380
HDAHPMGVLVSAMS+LS+FHPDANPALRGQDLYK KQVRDKQIARILGKAPTIAAAAYLR
Sbjct: 176 HDAHPMGVLVSAMSSLSIFHPDANPALRGQDLYKSKQVRDKQIARILGKAPTIAAAAYLR 235
Query: 1379 LAGRPPVLPSNNLSYSENFLYMLDSLGNHAYKPNPRLARVLDILFILHAEHEMNCSTAAA 1200
LAGRPPVLPS+NLSYSENFLYMLDSLGN +YKPNPRLARVLDILFILHAEHEMNCST+AA
Sbjct: 236 LAGRPPVLPSSNLSYSENFLYMLDSLGNRSYKPNPRLARVLDILFILHAEHEMNCSTSAA 295
Query: 1199 RHLASSGVDVFTALSGAVGALYGPLHGGANEAVLKMLNEIGTVENIPEFIEGVKNRKRKM 1020
RHLASSGVDV+TAL+GAVGALYGPLHGGANEAVLKML+EIGT+ENIP+FIEGVKNRKRKM
Sbjct: 296 RHLASSGVDVYTALAGAVGALYGPLHGGANEAVLKMLSEIGTIENIPDFIEGVKNRKRKM 355
Query: 1019 SGFGHRV 999
SGFGHRV
Sbjct: 356 SGFGHRV 362
Score = 228 bits (582), Expect = 4e-57
Identities = 109/120 (90%), Positives = 114/120 (95%)
Frame = -2
Query: 864 DPLIEVAVALEKAALTDEYFIKRKLYPNVDFYSGLIYRAMGFPTEFFPVLFAIPRMAGYL 685
DPLIEVAVALEKAAL DEYF+KRKLYPNVDFYSGLIYRAMGFPTEFFPVLFAIPRMAGYL
Sbjct: 387 DPLIEVAVALEKAALADEYFVKRKLYPNVDFYSGLIYRAMGFPTEFFPVLFAIPRMAGYL 446
Query: 684 AHWRESLDDPDTKIMRPQQVYTGVWLRHYMPFRERTMPTEADSLGQVSVSNASRRRLAGS 505
AHWRESLDDPDTKIMRPQQVYTG WLRHYMP +ER + T+AD LGQVS+SNASRRRLAGS
Sbjct: 447 AHWRESLDDPDTKIMRPQQVYTGEWLRHYMPLKERMIQTDADRLGQVSISNASRRRLAGS 506
Score = 93.6 bits (231), Expect = 2e-16
Identities = 54/87 (62%), Positives = 63/87 (72%), Gaps = 3/87 (3%)
Frame = -2
Query: 2115 LARGRLAVIAAHLATTSISPAL---LETSPVSAQVAARAPSALGGVLTVIDERTGKKYEI 1945
+ARGRLAV+ AHL + + ++ VSAQV P L GVLTVIDERTGKKY+I
Sbjct: 7 IARGRLAVLTAHLLGAPLESSEDSDIQRWCVSAQVPT--PGDLKGVLTVIDERTGKKYQI 64
Query: 1944 KVSDEGTVKASDFKKISTGNQDKGLKL 1864
+VS +GTVKASDFKKISTG DKGLKL
Sbjct: 65 QVSQDGTVKASDFKKISTGKSDKGLKL 91
>dbj|BAJ34181.1| unnamed protein product [Thellungiella halophila]
Length = 508
Score = 466 bits (1199), Expect = e-128
Identities = 228/247 (92%), Positives = 242/247 (97%)
Frame = -3
Query: 1739 LRYRGYPIEELAESSTFTEVAYLLMYGNLPSQNQLADWEFAISQHSAVPQGLLDIIQAMP 1560
LRYRGYPIEELAESSTF EVAYLLMYGNLPSQ+QLADWEFA+SQHSAVPQG+LDIIQ+MP
Sbjct: 116 LRYRGYPIEELAESSTFIEVAYLLMYGNLPSQSQLADWEFAVSQHSAVPQGVLDIIQSMP 175
Query: 1559 HDAHPMGVLVSAMSALSVFHPDANPALRGQDLYKLKQVRDKQIARILGKAPTIAAAAYLR 1380
HDAHPMGVLVSAMSALS+FHPDANPALRGQD+YK KQVRDKQI RILGKAPTIAAAAYLR
Sbjct: 176 HDAHPMGVLVSAMSALSIFHPDANPALRGQDIYKSKQVRDKQIVRILGKAPTIAAAAYLR 235
Query: 1379 LAGRPPVLPSNNLSYSENFLYMLDSLGNHAYKPNPRLARVLDILFILHAEHEMNCSTAAA 1200
+AGRPPVLPS NLSY+ENFLYMLDS+GN +YKPNPRLARVLDILFILHAEHEMNCSTAAA
Sbjct: 236 MAGRPPVLPSGNLSYAENFLYMLDSMGNRSYKPNPRLARVLDILFILHAEHEMNCSTAAA 295
Query: 1199 RHLASSGVDVFTALSGAVGALYGPLHGGANEAVLKMLNEIGTVENIPEFIEGVKNRKRKM 1020
RHLASSGVDV+TA++GAVGALYGPLHGGANEAVLKML+EIGTVENIPEFIEGVKNRKRKM
Sbjct: 296 RHLASSGVDVYTAVAGAVGALYGPLHGGANEAVLKMLSEIGTVENIPEFIEGVKNRKRKM 355
Query: 1019 SGFGHRV 999
SGFGHRV
Sbjct: 356 SGFGHRV 362
Score = 221 bits (562), Expect = 9e-55
Identities = 104/121 (85%), Positives = 114/121 (94%)
Frame = -2
Query: 864 DPLIEVAVALEKAALTDEYFIKRKLYPNVDFYSGLIYRAMGFPTEFFPVLFAIPRMAGYL 685
DPLIEVAVALEKAAL+DEYF+KRKLYPNVDFYSGLIYRAMGFP EFF VLFAIPRMAGYL
Sbjct: 387 DPLIEVAVALEKAALSDEYFVKRKLYPNVDFYSGLIYRAMGFPPEFFTVLFAIPRMAGYL 446
Query: 684 AHWRESLDDPDTKIMRPQQVYTGVWLRHYMPFRERTMPTEADSLGQVSVSNASRRRLAGS 505
+HWRESLDDPDTKIMRPQQ YTGVWLRHY P RERT+ +++D LGQVS+SNASRRRL+GS
Sbjct: 447 SHWRESLDDPDTKIMRPQQAYTGVWLRHYEPVRERTLSSDSDKLGQVSISNASRRRLSGS 506
Query: 504 S 502
+
Sbjct: 507 A 507
Score = 77.4 bits (189), Expect = 2e-11
Identities = 44/89 (49%), Positives = 57/89 (64%), Gaps = 3/89 (3%)
Frame = -2
Query: 2121 ADLARGRLAVIAAHLATTS---ISPALLETSPVSAQVAARAPSALGGVLTVIDERTGKKY 1951
++ + RLAV+ AHLA + + L P AA S L G LT++DERTGKKY
Sbjct: 4 SERVKARLAVLTAHLAVSDPVGLEQVLPAIEPWCTSSAAPHGS-LKGSLTIVDERTGKKY 62
Query: 1950 EIKVSDEGTVKASDFKKISTGNQDKGLKL 1864
++ VS++GTVKA D KKI+TG DKGLKL
Sbjct: 63 QVPVSEDGTVKAVDLKKITTGKDDKGLKL 91