BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dioscorea21_contig00000246
         (1478 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAM28972.1| flavonoid 3'-hydroxylase [Lilium hybrid division I]   490   e-136
dbj|BAM28971.1| flavonoid 3'-hydroxylase [Lilium hybrid division I]   484   e-134
dbj|BAH22519.1| flavonoid 3' hydroxylase [Tricyrtis hirta]            481   e-133
ref|NP_001064338.1| Os10g0320100 [Oryza sativa Japonica Group] g...   473   e-131
ref|XP_002284151.1| PREDICTED: flavonoid 3'-monooxygenase [Vitis...   473   e-131

>dbj|BAM28972.1| flavonoid 3'-hydroxylase [Lilium hybrid division I]
          Length = 514

 Score =  490 bits (1262), Expect = e-136
 Identities = 253/386 (65%), Positives = 290/386 (75%), Gaps = 6/386 (1%)
 Frame = -2

Query: 1141 LPPGPKGWPILGNLPQLGQKPHHTLQALSKTYGPLFRLRLGSIDXXXXXXXXXXXAFLRT 962
            LPPGPKGWPILGNLP LG KPH T+ ALSK +GPLFRLRLGS +            FLRT
Sbjct: 32   LPPGPKGWPILGNLPHLGPKPHQTMHALSKLHGPLFRLRLGSAEVIVAASASIASEFLRT 91

Query: 961  HDSNFSNRPPNSGAEHIAYNYQDLVFAPYGPRWRSLRRLCSLHLFSVKALEDXXXXXXXX 782
            HD+NFSNRPPNSGAEHIAYNY DLVFAPYGPRWR+LR+LC+LHLFS KALED        
Sbjct: 92   HDTNFSNRPPNSGAEHIAYNYNDLVFAPYGPRWRALRKLCALHLFSQKALEDLCYVREQE 151

Query: 781  XXXXXXXXVKMKQKTVNVGQAVNACATNALARATVGRRVFTXXXXXXXXE-FKAMVVELM 605
                         + VN+G+ +N CATNAL+RAT+G RVF         E FK MV+E+M
Sbjct: 152  VAILARDLAG-SSRPVNLGETLNVCATNALSRATIGWRVFVGKGEDVTAEEFKDMVMEVM 210

Query: 604  RLAGVFNVGDFVPWLRPLDLQGVVKKMKKLHRRYDEFFDKIIEEHRSDV----VVGGDLL 437
            RLAGVFN+GDFVP L  LDLQGVV KMKKLH+R D F DK+I EHR+ V    + G DLL
Sbjct: 211  RLAGVFNIGDFVPGLGWLDLQGVVGKMKKLHKRMDSFLDKLIAEHRAAVAGGELAGKDLL 270

Query: 436  SVMLALEELEDG-GGKLNDTEIKALLLNLFTAGTDTTASTVEWALAELLRHPDILAMAQS 260
            SVM+ L+E  +G GGKL DTEIKALLLNLFTAGTDTT+STVEWALAEL+RHP +L  AQ 
Sbjct: 271  SVMVRLQEEGEGEGGKLTDTEIKALLLNLFTAGTDTTSSTVEWALAELIRHPQLLHQAQK 330

Query: 259  ELDAVIGHGRLVADSDLHKLPLMQAIIKETLRLHPPTPLSLPRIASETCQVAGYQIPKSA 80
            ELD V+GH RLV++SDL  LP + AIIKET RLHP TPLSLPR+ASE+C + GY IPK+A
Sbjct: 331  ELDTVVGHDRLVSESDLPHLPFLSAIIKETFRLHPSTPLSLPRMASESCTINGYHIPKNA 390

Query: 79   TLFVNVWAIARDPEVWSDPLKFDPHR 2
            TL VNVWAIARDP VW+ P++F P R
Sbjct: 391  TLLVNVWAIARDPAVWAAPVEFKPDR 416


>dbj|BAM28971.1| flavonoid 3'-hydroxylase [Lilium hybrid division I]
          Length = 514

 Score =  484 bits (1247), Expect = e-134
 Identities = 250/386 (64%), Positives = 289/386 (74%), Gaps = 6/386 (1%)
 Frame = -2

Query: 1141 LPPGPKGWPILGNLPQLGQKPHHTLQALSKTYGPLFRLRLGSIDXXXXXXXXXXXAFLRT 962
            LPPGPKGWPILGNLP LG KPH T+ ALSK +GPLFRLRLGS +            FLRT
Sbjct: 32   LPPGPKGWPILGNLPHLGPKPHQTMHALSKLHGPLFRLRLGSAEVIVAASAPIASEFLRT 91

Query: 961  HDSNFSNRPPNSGAEHIAYNYQDLVFAPYGPRWRSLRRLCSLHLFSVKALEDXXXXXXXX 782
            HD+NFSNRPPNSGAEHIAYNY DLVFAPYGPRWR+LR+LC+LHLFS KALED        
Sbjct: 92   HDTNFSNRPPNSGAEHIAYNYNDLVFAPYGPRWRTLRKLCALHLFSQKALEDLCYVREQE 151

Query: 781  XXXXXXXXVKMKQKTVNVGQAVNACATNALARATVGRRVFTXXXXXXXXE-FKAMVVELM 605
                         + VN+G+ +N CATNAL+RAT+G RVF         E FK MV+E+M
Sbjct: 152  VAILARDLAG-SCRPVNLGETLNVCATNALSRATIGWRVFVGKGEDVTAEEFKDMVMEVM 210

Query: 604  RLAGVFNVGDFVPWLRPLDLQGVVKKMKKLHRRYDEFFDKIIEEHRSDV----VVGGDLL 437
            RLAGVFN+GDFVP L  LDLQGVV KMKKLH+R D F DK+I EHR+ V    + G  LL
Sbjct: 211  RLAGVFNIGDFVPGLGWLDLQGVVGKMKKLHKRMDAFLDKLIAEHRAAVAGGELAGKHLL 270

Query: 436  SVMLALEELEDG-GGKLNDTEIKALLLNLFTAGTDTTASTVEWALAELLRHPDILAMAQS 260
            SVM+ L+E  +G GGKL DTEIKALLLNLFTAGTDTT+STVEWALAEL+RHP +L  AQ 
Sbjct: 271  SVMVRLQEEGEGEGGKLTDTEIKALLLNLFTAGTDTTSSTVEWALAELIRHPQLLHQAQK 330

Query: 259  ELDAVIGHGRLVADSDLHKLPLMQAIIKETLRLHPPTPLSLPRIASETCQVAGYQIPKSA 80
            ELD V+GH RLV++SDL  LP + A+IKET RLHP TPL+LPR+ASE+C + GY IPK+A
Sbjct: 331  ELDTVVGHDRLVSESDLPHLPFLSAVIKETFRLHPSTPLALPRMASESCTINGYHIPKNA 390

Query: 79   TLFVNVWAIARDPEVWSDPLKFDPHR 2
            TL VNVWAIARDP VW+ P++F P R
Sbjct: 391  TLLVNVWAIARDPAVWAAPVEFKPDR 416


>dbj|BAH22519.1| flavonoid 3' hydroxylase [Tricyrtis hirta]
          Length = 505

 Score =  481 bits (1238), Expect = e-133
 Identities = 252/383 (65%), Positives = 289/383 (75%), Gaps = 3/383 (0%)
 Frame = -2

Query: 1141 LPPGPKGWPILGNLPQLGQKPHHTLQALSKTYGPLFRLRLGSIDXXXXXXXXXXXAFLRT 962
            LPPGP+GWPILGNLPQLG KPH TL ALSK +GPLF LRLGS+D           AFLR 
Sbjct: 29   LPPGPRGWPILGNLPQLGPKPHQTLHALSKAHGPLFLLRLGSVDVVVAASAAVAAAFLRQ 88

Query: 961  HDSNFSNRPPNSGAEHIAYNYQDLVFAPYGPRWRSLRRLCSLHLFSVKALEDXXXXXXXX 782
            HD+ FSNRPPNSGAEHIAYNYQDLVFAPYGPRWR LR+LCSLHLFS KAL+D        
Sbjct: 89   HDAIFSNRPPNSGAEHIAYNYQDLVFAPYGPRWRHLRKLCSLHLFSSKALDDLRPIREQE 148

Query: 781  XXXXXXXXVKMKQKTVNVGQAVNACATNALARATVGRRVFTXXXXXXXXE-FKAMVVELM 605
                      +  + VN+G+AVN CATNALARATVG RVF         E FK MV+E+M
Sbjct: 149  IQRLTRSL--LVGRPVNLGEAVNVCATNALARATVGWRVFVGEGEDTAAEEFKEMVLEIM 206

Query: 604  RLAGVFNVGDFVPWLRPLDLQGVVKKMKKLHRRYDEFFDKIIEEHRSDVVVGGDLLSVML 425
            +LAGVFN+GDFVP +  +DLQGVV KMKKLH+R D F DK+I EH+     G DLLSV++
Sbjct: 207  KLAGVFNIGDFVPGIGWMDLQGVVGKMKKLHKRMDAFLDKLINEHKRGSG-GRDLLSVLV 265

Query: 424  AL--EELEDGGGKLNDTEIKALLLNLFTAGTDTTASTVEWALAELLRHPDILAMAQSELD 251
            +L  +E ED   KL+ TEIKALLLNLFTAGTDTT+STVEWALAEL+RHP +L  AQ ELD
Sbjct: 266  SLKDDEGEDEANKLSHTEIKALLLNLFTAGTDTTSSTVEWALAELIRHPTLLHKAQQELD 325

Query: 250  AVIGHGRLVADSDLHKLPLMQAIIKETLRLHPPTPLSLPRIASETCQVAGYQIPKSATLF 71
             V+G  RLV+++DL  LP +QAIIKET RLHP TPLSLPRI+SE C++ GY IPK+ATL 
Sbjct: 326  NVVGRQRLVSETDLPNLPFLQAIIKETFRLHPSTPLSLPRISSEPCEINGYYIPKNATLL 385

Query: 70   VNVWAIARDPEVWSDPLKFDPHR 2
            VNVWAIARDP VWSDPL+F P R
Sbjct: 386  VNVWAIARDPAVWSDPLEFKPER 408


>ref|NP_001064338.1| Os10g0320100 [Oryza sativa Japonica Group]
            gi|19881547|gb|AAM00948.1|AC021892_12 Putative flavonoid
            3'-hydroxylase [Oryza sativa Japonica Group]
            gi|22725927|gb|AAN04937.1| Putative chalcone flavonoid 3'
            - hydroxylase [Oryza sativa Japonica Group]
            gi|31431083|gb|AAP52914.1| Flavonoid 3'-monooxygenase,
            putative, expressed [Oryza sativa Japonica Group]
            gi|113638947|dbj|BAF26252.1| Os10g0320100 [Oryza sativa
            Japonica Group] gi|125531442|gb|EAY78007.1| hypothetical
            protein OsI_33047 [Oryza sativa Indica Group]
            gi|125574353|gb|EAZ15637.1| hypothetical protein
            OsJ_31048 [Oryza sativa Japonica Group]
            gi|215693977|dbj|BAG89180.1| unnamed protein product
            [Oryza sativa Japonica Group] gi|340343700|gb|AEK31169.1|
            flavonoid 3'-hydroxylase [Oryza sativa Japonica Group]
          Length = 526

 Score =  473 bits (1218), Expect = e-131
 Identities = 241/392 (61%), Positives = 284/392 (72%), Gaps = 12/392 (3%)
 Frame = -2

Query: 1141 LPPGPKGWPILGNLPQLGQKPHHTLQALSKTYGPLFRLRLGSIDXXXXXXXXXXXAFLRT 962
            LPPGP+GWP+LGNLPQLG KPHHT+ AL++ YGPLFRLR G  +            FLR 
Sbjct: 41   LPPGPRGWPVLGNLPQLGDKPHHTMCALARQYGPLFRLRFGCAEVVVAASAPVAAQFLRG 100

Query: 961  HDSNFSNRPPNSGAEHIAYNYQDLVFAPYGPRWRSLRRLCSLHLFSVKALEDXXXXXXXX 782
            HD+NFSNRPPNSGAEH+AYNYQDLVFAPYG RWR+LR+LC+LHLFS KAL+D        
Sbjct: 101  HDANFSNRPPNSGAEHVAYNYQDLVFAPYGARWRALRKLCALHLFSAKALDDLRAVREGE 160

Query: 781  XXXXXXXXVKMKQKTVNVGQAVNACATNALARATVGRRVFTXXXXXXXXEFKAMVVELMR 602
                     + +  +V +GQ  N CATN LARAT+G RVF         EFK MVVELM+
Sbjct: 161  VALMVRNLARQQAASVALGQEANVCATNTLARATIGHRVFAVDGGEGAREFKEMVVELMQ 220

Query: 601  LAGVFNVGDFVPWLRPLDLQGVVKKMKKLHRRYDEFFDKIIEEHRS----DVVVGG---- 446
            LAGVFNVGDFVP LR LD QGVV KMK+LHRRYD   +  I E ++    D V  G    
Sbjct: 221  LAGVFNVGDFVPALRWLDPQGVVAKMKRLHRRYDNMMNGFINERKAGAQPDGVAAGEHGN 280

Query: 445  DLLSVMLAL----EELEDGGGKLNDTEIKALLLNLFTAGTDTTASTVEWALAELLRHPDI 278
            DLLSV+LA     ++L+  G K+ +T+IKALLLNLFTAGTDTT+STVEWALAEL+RHPD+
Sbjct: 281  DLLSVLLARMQEEQKLDGDGEKITETDIKALLLNLFTAGTDTTSSTVEWALAELIRHPDV 340

Query: 277  LAMAQSELDAVIGHGRLVADSDLHKLPLMQAIIKETLRLHPPTPLSLPRIASETCQVAGY 98
            L  AQ ELD V+G GRLV++SDL +LP + A+IKET RLHP TPLSLPR A+E C+V GY
Sbjct: 341  LKEAQHELDTVVGRGRLVSESDLPRLPYLTAVIKETFRLHPSTPLSLPREAAEECEVDGY 400

Query: 97   QIPKSATLFVNVWAIARDPEVWSDPLKFDPHR 2
            +IPK ATL VNVWAIARDP  W DPL++ P R
Sbjct: 401  RIPKGATLLVNVWAIARDPTQWPDPLQYQPSR 432


>ref|XP_002284151.1| PREDICTED: flavonoid 3'-monooxygenase [Vitis vinifera]
            gi|147801850|emb|CAN75347.1| hypothetical protein
            VITISV_002956 [Vitis vinifera]
          Length = 509

 Score =  473 bits (1216), Expect = e-131
 Identities = 237/388 (61%), Positives = 284/388 (73%), Gaps = 8/388 (2%)
 Frame = -2

Query: 1141 LPPGPKGWPILGNLPQLGQKPHHTLQALSKTYGPLFRLRLGSIDXXXXXXXXXXXAFLRT 962
            LPPGPK WPI+GNLP LG  PHH++ AL+KTYGPL  LR+G +D            FL+T
Sbjct: 28   LPPGPKPWPIVGNLPHLGPVPHHSIAALAKTYGPLMHLRMGFVDVVVAASASVAAQFLKT 87

Query: 961  HDSNFSNRPPNSGAEHIAYNYQDLVFAPYGPRWRSLRRLCSLHLFSVKALEDXXXXXXXX 782
            HD+NFSNRPPNSGA+HIAYNYQDLVFAPYGPRWR LR++CS+HLFS +AL+D        
Sbjct: 88   HDANFSNRPPNSGAKHIAYNYQDLVFAPYGPRWRMLRKICSVHLFSGQALDDFRHIRQEE 147

Query: 781  XXXXXXXXVKMKQKTVNVGQAVNACATNALARATVGRRVF---TXXXXXXXXEFKAMVVE 611
                     +  Q  V +GQ +N C TNAL R  +GRRVF   +        EFK MVVE
Sbjct: 148  VLALMRALARAGQTPVKLGQLLNVCTTNALGRVMLGRRVFGDGSGGEDPKADEFKEMVVE 207

Query: 610  LMRLAGVFNVGDFVPWLRPLDLQGVVKKMKKLHRRYDEFFDKIIEEHRSDVVVGG----D 443
            LM LAGVFN+GDFVP L  LDLQGV  KMKKLH R+D F   I+EEH+     G     D
Sbjct: 208  LMVLAGVFNIGDFVPALEWLDLQGVASKMKKLHARFDAFLGAIVEEHKISGSAGSERHVD 267

Query: 442  LLSVMLALEELEDG-GGKLNDTEIKALLLNLFTAGTDTTASTVEWALAELLRHPDILAMA 266
            LLS +++L++  DG GGKL D EIKALLLNLFTAGTDT++STVEWA+AEL+RHP+++A A
Sbjct: 268  LLSTLISLKDNADGEGGKLTDVEIKALLLNLFTAGTDTSSSTVEWAIAELIRHPEMMAQA 327

Query: 265  QSELDAVIGHGRLVADSDLHKLPLMQAIIKETLRLHPPTPLSLPRIASETCQVAGYQIPK 86
            Q ELDAV+G GRLV D DL KL  +QAI+KET RLHP TPLSLPR+A+E+C++ GY IPK
Sbjct: 328  QQELDAVVGRGRLVTDLDLPKLTYLQAIVKETFRLHPSTPLSLPRMAAESCEINGYHIPK 387

Query: 85   SATLFVNVWAIARDPEVWSDPLKFDPHR 2
            +ATL VNVWAIARDPEVW +PL+F P+R
Sbjct: 388  NATLLVNVWAIARDPEVWEEPLEFRPNR 415