BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00021715
         (1234 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P81898.2|PNAA_PRUDU RecName: Full=Peptide-N4-(N-acetyl-beta-g...   626   e-177
ref|XP_002285454.1| PREDICTED: peptide-N4-(N-acetyl-beta-glucosa...   621   e-175
ref|XP_002316856.1| predicted protein [Populus trichocarpa] gi|2...   616   e-174
ref|XP_002524519.1| Peptide-N4-(N-acetyl-beta-glucosaminyl)aspar...   594   e-167
ref|XP_004146092.1| PREDICTED: peptide-N4-(N-acetyl-beta-glucosa...   587   e-165

>sp|P81898.2|PNAA_PRUDU RecName: Full=Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine
            amidase A; Short=PNGase A; AltName: Full=Glycopeptide
            N-glycosidase; AltName: Full=N-glycanase; Contains:
            RecName:
            Full=Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine
            amidase A light chain; AltName: Full=PNGase A small
            chain; AltName: Full=PNGase A subunit B; Contains:
            RecName:
            Full=Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine
            amidase A heavy chain; AltName: Full=PNGase A large
            chain; Short=PNGase A subunit A
          Length = 571

 Score =  626 bits (1614), Expect = e-177
 Identities = 290/411 (70%), Positives = 346/411 (84%)
 Frame = +2

Query: 2    DIPITKYFQVTKPIKLPKTKPCSTLVLQHDFGYTYGKPPVLANYNPPKNCPSKNFSKIVL 181
            D P T +F+VTKPI++PKTKPCS L+LQHDF YTYG+ PV ANY PP +CPS+ FS IVL
Sbjct: 7    DTPPTVFFEVTKPIEVPKTKPCSQLILQHDFAYTYGQAPVFANYTPPSDCPSQTFSTIVL 66

Query: 182  EWKATCKGRQFDRIFGVWLGGVEIFRSCTAEPRATGIVWSVEKDITRYSSLLKNPQTLAV 361
            EWKATC+ RQFDRIFGVWLGGVEI RSCTAEPR  GIVW+VEKDITRY SLLK+ QTLAV
Sbjct: 67   EWKATCRRRQFDRIFGVWLGGVEILRSCTAEPRPNGIVWTVEKDITRYYSLLKSNQTLAV 126

Query: 362  YLGNLVDSTYTGIYHVNVTFHFYPAELEQHLHGFNRHFGNLEFGYDSSADLVLPISRNLP 541
            YLGNL+D TYTGIYHVN++ HFYPA+  + L+ F +   NL  GY S ADL+LPISRNLP
Sbjct: 127  YLGNLIDKTYTGIYHVNISLHFYPAK--EKLNSFQQKLDNLASGYHSWADLILPISRNLP 184

Query: 542  LNDGQWFLVQNSTDVQLKEFTIPRNAYRAVLEVYVSFHQNDEFWYGNPPNDYIVANNLTG 721
            LNDG WF VQNS D +LKEF IP+NAYRAVLEVYVSFH+NDEFWY N PN+YI ANNL+G
Sbjct: 185  LNDGLWFEVQNSNDTELKEFKIPQNAYRAVLEVYVSFHENDEFWYSNLPNEYIAANNLSG 244

Query: 722  LAGNGPFREVLISLDGKAVGAVYPFTVIYTGGVNPLLWRPITGIGSFDLPSYDIEVTPFL 901
              GNGPFREV++SLDG+ VGAV+PFTVI+TGG+NPLLWRPIT IGSFDLP+YDIE+TPFL
Sbjct: 245  TPGNGPFREVVVSLDGEVVGAVWPFTVIFTGGINPLLWRPITAIGSFDLPTYDIEITPFL 304

Query: 902  GRILDGMEHKFGFGVTNALDVWYIDANLHIWLDSKSVETKGNLIEYKSPTPAVSLVSNFS 1081
            G+ILDG  HKFGF VTNAL+VWY+DANLH+WLD +S +T+G L ++ S    VSLVS+F 
Sbjct: 305  GKILDGKSHKFGFNVTNALNVWYVDANLHLWLDKQSTKTEGKLSKHSSLPLVVSLVSDFK 364

Query: 1082 GLDGTFLTKASRSIYSSGWVESSHGKVVTHSFQEFSFTNFMRMGKNGSLQI 1234
            GL+GTFLT+ SRS+ S+GWV+SS+G + T S Q+F ++N M +GK+G++QI
Sbjct: 365  GLNGTFLTRTSRSVSSTGWVKSSYGNITTRSIQDFYYSNSMVLGKDGNMQI 415


>ref|XP_002285454.1| PREDICTED: peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase
            A-like [Vitis vinifera]
          Length = 612

 Score =  621 bits (1601), Expect = e-175
 Identities = 293/410 (71%), Positives = 339/410 (82%)
 Frame = +2

Query: 2    DIPITKYFQVTKPIKLPKTKPCSTLVLQHDFGYTYGKPPVLANYNPPKNCPSKNFSKIVL 181
            D P  ++F+VTKPIK+PKTKPCS+LVLQHDFGYTYGKPPVLA Y+PP +CPS+NFSKIVL
Sbjct: 46   DTPPIRFFEVTKPIKVPKTKPCSSLVLQHDFGYTYGKPPVLAPYSPPAHCPSQNFSKIVL 105

Query: 182  EWKATCKGRQFDRIFGVWLGGVEIFRSCTAEPRATGIVWSVEKDITRYSSLLKNPQTLAV 361
            EW ATCKGRQFDRIFGVWLGGVE+ RSCTAEP ATGIVW+V+KD+TRY SLL   +TLAV
Sbjct: 106  EWSATCKGRQFDRIFGVWLGGVELLRSCTAEPTATGIVWTVKKDVTRYYSLLMKEETLAV 165

Query: 362  YLGNLVDSTYTGIYHVNVTFHFYPAELEQHLHGFNRHFGNLEFGYDSSADLVLPISRNLP 541
            YLGN+VD TYTG+YHVNVTFHFYPA          ++   L  GY S ADL+LPISRNLP
Sbjct: 166  YLGNVVDKTYTGVYHVNVTFHFYPAA---------KNSEKLASGYGSWADLILPISRNLP 216

Query: 542  LNDGQWFLVQNSTDVQLKEFTIPRNAYRAVLEVYVSFHQNDEFWYGNPPNDYIVANNLTG 721
            LNDG WF ++NSTD+++K+F IPRNAYRAVLEVY+SFH+NDEFWY NPPNDYI ANNLTG
Sbjct: 217  LNDGLWFEIENSTDLEVKKFKIPRNAYRAVLEVYLSFHENDEFWYSNPPNDYISANNLTG 276

Query: 722  LAGNGPFREVLISLDGKAVGAVYPFTVIYTGGVNPLLWRPITGIGSFDLPSYDIEVTPFL 901
              GNGPFREVL+ LDGK VGAV+PFTVIYTGGVNPLLWRPITGIGSFDLPSY+IE+TPFL
Sbjct: 277  TPGNGPFREVLVGLDGKLVGAVWPFTVIYTGGVNPLLWRPITGIGSFDLPSYNIEITPFL 336

Query: 902  GRILDGMEHKFGFGVTNALDVWYIDANLHIWLDSKSVETKGNLIEYKSPTPAVSLVSNFS 1081
            G+ILDG  H F F VTNAL+VWYIDANLHIWLD KS +T+G L+ + S +   +      
Sbjct: 337  GKILDGKTHTFEFSVTNALNVWYIDANLHIWLDHKSTQTEGRLLGHGSGSSLSTSTLYMK 396

Query: 1082 GLDGTFLTKASRSIYSSGWVESSHGKVVTHSFQEFSFTNFMRMGKNGSLQ 1231
            GL+ +FLT +SRSI S+GWV+SSHGK+ T S QEF + N M MG +G LQ
Sbjct: 397  GLNASFLTLSSRSISSTGWVKSSHGKMTTQSIQEFKYINLMVMGNDGDLQ 446


>ref|XP_002316856.1| predicted protein [Populus trichocarpa] gi|222859921|gb|EEE97468.1|
            predicted protein [Populus trichocarpa]
          Length = 631

 Score =  616 bits (1589), Expect = e-174
 Identities = 293/415 (70%), Positives = 338/415 (81%), Gaps = 4/415 (0%)
 Frame = +2

Query: 2    DIPITKYFQVTKPIKLPKTKPCSTLVLQHDFGYTYGKPPVLANYNPPKNCPSKNFSKIVL 181
            D P T +F+VTKPI +P TKPC   +LQHDF  TYGKPPVL NY PP +CPS++FSKIVL
Sbjct: 55   DTPPTVFFEVTKPIDVPNTKPCKHFILQHDFASTYGKPPVLVNYTPPSHCPSQDFSKIVL 114

Query: 182  EWKATCKGRQFDRIFGVWLGGVEIFRSCTAEPRATGIVWSVEKDITRYSSLLKNPQT--L 355
            EWKATCKGRQFDRIFGVWLGGVE+ RSCTAEPRATGIVW+V KDITRY SLL   +T   
Sbjct: 115  EWKATCKGRQFDRIFGVWLGGVELLRSCTAEPRATGIVWTVRKDITRYYSLLVKNETQEF 174

Query: 356  AVYLGNLVDSTYTGIYHVNVTFHFYPAE--LEQHLHGFNRHFGNLEFGYDSSADLVLPIS 529
            AVY+GN+VDSTYTGIYHVNV+ +FYPAE  L    HGFN    NL  G DS ADL+LPIS
Sbjct: 175  AVYMGNIVDSTYTGIYHVNVSIYFYPAEKKLSHSDHGFN----NLASGRDSKADLILPIS 230

Query: 530  RNLPLNDGQWFLVQNSTDVQLKEFTIPRNAYRAVLEVYVSFHQNDEFWYGNPPNDYIVAN 709
            RN PLNDG WF +QNSTD + KEF IP+N YRAVLEVYVSFH+NDEFWYGN PN+YI+AN
Sbjct: 231  RNFPLNDGFWFEIQNSTDSEAKEFKIPQNVYRAVLEVYVSFHENDEFWYGNYPNEYIIAN 290

Query: 710  NLTGLAGNGPFREVLISLDGKAVGAVYPFTVIYTGGVNPLLWRPITGIGSFDLPSYDIEV 889
            NLTG  GNGPFREV++SLDG+ VGAV+PFTV++TGG+NPLLWRPIT IGSFDLPSYDIE+
Sbjct: 291  NLTGFPGNGPFREVVVSLDGEIVGAVWPFTVVFTGGINPLLWRPITAIGSFDLPSYDIEI 350

Query: 890  TPFLGRILDGMEHKFGFGVTNALDVWYIDANLHIWLDSKSVETKGNLIEYKSPTPAVSLV 1069
            TPFLG ILDG  HK GF VTNAL+VWYIDANLH+WLD +S  T+G L++++S   A+SLV
Sbjct: 351  TPFLGNILDGKTHKLGFSVTNALNVWYIDANLHLWLDHRSTITEGKLLKHESKPLALSLV 410

Query: 1070 SNFSGLDGTFLTKASRSIYSSGWVESSHGKVVTHSFQEFSFTNFMRMGKNGSLQI 1234
            SNF+GL+G FLT A R I S+GWV+SSHG + T   Q F + N M MGK+G LQI
Sbjct: 411  SNFTGLNGKFLTSARRFISSNGWVKSSHGNITTRFNQHFGYRNLMEMGKDGDLQI 465


>ref|XP_002524519.1| Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A, putative
            [Ricinus communis] gi|223536193|gb|EEF37846.1|
            Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase
            A, putative [Ricinus communis]
          Length = 624

 Score =  594 bits (1532), Expect = e-167
 Identities = 284/409 (69%), Positives = 333/409 (81%), Gaps = 2/409 (0%)
 Frame = +2

Query: 14   TKYFQVTKPIKLPKTKPCSTLVLQHDFGYTYGKPPVLANYNPPKNCPSKNFSKIVLEWKA 193
            T YF+VTKPI +P TKPC   VLQHDFGYTYGK PVLANY PP +CPS++FSKI+LEW A
Sbjct: 53   TVYFEVTKPIHVPNTKPCIHTVLQHDFGYTYGKSPVLANYTPPSHCPSQHFSKIILEWNA 112

Query: 194  TCKGRQFDRIFGVWLGGVEIFRSCTAEPRATGIVWSVEKDITRY-SSLLKNP-QTLAVYL 367
            TCKGRQFDRIFGVWLGGVE+ RSCTAEPRATGI WSV+KDITRY SSLLK+  Q LAVYL
Sbjct: 113  TCKGRQFDRIFGVWLGGVELLRSCTAEPRATGIFWSVQKDITRYYSSLLKDETQELAVYL 172

Query: 368  GNLVDSTYTGIYHVNVTFHFYPAELEQHLHGFNRHFGNLEFGYDSSADLVLPISRNLPLN 547
            GNLVDSTYTG+Y VNVT +FYPAE ++  +  N    + +  +DS ADL+LPISR+LPLN
Sbjct: 173  GNLVDSTYTGVYRVNVTLYFYPAE-DKSSYNENSLLDHFKARHDSKADLILPISRDLPLN 231

Query: 548  DGQWFLVQNSTDVQLKEFTIPRNAYRAVLEVYVSFHQNDEFWYGNPPNDYIVANNLTGLA 727
            DG WF +QNSTD QLKEF IP N YRAVLEVYVSFH+NDEFWY N  N+YI ANNLTG  
Sbjct: 232  DGLWFQIQNSTDTQLKEFEIPPNVYRAVLEVYVSFHENDEFWYSNYYNEYISANNLTGSP 291

Query: 728  GNGPFREVLISLDGKAVGAVYPFTVIYTGGVNPLLWRPITGIGSFDLPSYDIEVTPFLGR 907
            GNGPFREV++SLDG+ +GA++PFTVIYTGG+NPLLWRPIT IGSFDLPSYD+E+TP LG 
Sbjct: 292  GNGPFREVIVSLDGEVLGAIWPFTVIYTGGINPLLWRPITAIGSFDLPSYDLEMTPLLGS 351

Query: 908  ILDGMEHKFGFGVTNALDVWYIDANLHIWLDSKSVETKGNLIEYKSPTPAVSLVSNFSGL 1087
            +LDG  HK GF VTNAL+VWYIDANLH+WLD KS + +G +++++    A SL+SNF  L
Sbjct: 352  VLDGKTHKLGFSVTNALNVWYIDANLHLWLDHKSKKIEGKVLKHEGKPLAFSLISNFKDL 411

Query: 1088 DGTFLTKASRSIYSSGWVESSHGKVVTHSFQEFSFTNFMRMGKNGSLQI 1234
            +GTFL  A RSI S+GWV+ S GK+ TH  Q FS+ N M MG +G+LQI
Sbjct: 412  NGTFLAAAQRSISSTGWVKCSFGKITTHFNQRFSYNNSMEMGDDGNLQI 460


>ref|XP_004146092.1| PREDICTED: peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase
            A-like, partial [Cucumis sativus]
          Length = 624

 Score =  587 bits (1512), Expect = e-165
 Identities = 280/408 (68%), Positives = 328/408 (80%)
 Frame = +2

Query: 8    PITKYFQVTKPIKLPKTKPCSTLVLQHDFGYTYGKPPVLANYNPPKNCPSKNFSKIVLEW 187
            P T +F+V+KPI  PK+KPCS L+L HDF +TYGKPPVLA+Y PP +C    FSKIVLEW
Sbjct: 54   PPTVFFEVSKPIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEW 113

Query: 188  KATCKGRQFDRIFGVWLGGVEIFRSCTAEPRATGIVWSVEKDITRYSSLLKNPQTLAVYL 367
            KATC+GRQFDRIFGVWLGGVEI RSCTAEPRATGIVW+V+KDITRYSSLL   QTLAVYL
Sbjct: 114  KATCRGRQFDRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYL 173

Query: 368  GNLVDSTYTGIYHVNVTFHFYPAELEQHLHGFNRHFGNLEFGYDSSADLVLPISRNLPLN 547
            GNLVD TYTGIY+V +  HFYP E     +G         +  DS ADL+LPISRNLPLN
Sbjct: 174  GNLVDKTYTGIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLN 233

Query: 548  DGQWFLVQNSTDVQLKEFTIPRNAYRAVLEVYVSFHQNDEFWYGNPPNDYIVANNLTGLA 727
            DG WF VQNSTDV+ KEF IP+N YRAVLEVYVSFH+NDEFWY N PNDYI+ANNLT   
Sbjct: 234  DGLWFEVQNSTDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTP 293

Query: 728  GNGPFREVLISLDGKAVGAVYPFTVIYTGGVNPLLWRPITGIGSFDLPSYDIEVTPFLGR 907
            GNGPFREVL+ LDG+ VGAV+PFTVIYTGGVNPLLWRPI+GIGSF+LPSYDIE+TPFLG 
Sbjct: 294  GNGPFREVLVDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGN 353

Query: 908  ILDGMEHKFGFGVTNALDVWYIDANLHIWLDSKSVETKGNLIEYKSPTPAVSLVSNFSGL 1087
            +LDG  H FGF VT+AL+VW+I+ANLH+WLD  SV+T+  L+ +   +P++S   NF+G 
Sbjct: 354  LLDGKVHNFGFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGP 413

Query: 1088 DGTFLTKASRSIYSSGWVESSHGKVVTHSFQEFSFTNFMRMGKNGSLQ 1231
            DGTFLTK +RS+ S+GWV+SS G + T S Q+ S++N M MG NGS Q
Sbjct: 414  DGTFLTKVTRSVSSTGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQ 461